A tea leaf tea amino acid composition for relieving anxiety, a preparation method and applications thereof

Abstract

The present application relates to a kind of tea tea amino acid compositions for relieving anxiety, preparation method and its application, belong to ordinary food and health food technical field, including the following preparation steps: tea tea amino acid is combined with composite carrier, obtains carrier composite material;By carrier composite material to plant extract is included, obtains inclusion material;After inclusion material and ternary synergistic compound, compound vitamin are mixed, granulation, obtains tea tea amino acid composition for relieving anxiety.In the present application technical scheme, by tea tea amino acid and composite carrier are combined, the stability and bioavailability of tea tea amino acid composition are enhanced, by carrier composite material to plant extract is included, the sustained-release performance and the performance of relieving anxiety of tea tea amino acid composition are enhanced, ternary synergistic compound further improves the relieving anxiety effect of tea tea amino acid composition, overall improves the comprehensive performance of tea tea amino acid composition.

Description

Technical Field

[0001] This invention belongs to the technical field of general food and health food, specifically relating to a tea theanine composition for relieving anxiety, its preparation method, and its application. Background Technology

[0002] With the fast pace of modern life and increasing work pressure, anxiety disorders have become a common mental health problem. Long-term anxiety not only affects mood and sleep quality but can also lead to memory loss, weakened immunity, and cardiovascular disease. Therefore, developing safe, effective, and non-addictive anti-anxiety interventions is of significant social and clinical importance. Theanine, a unique natural amino acid found in tea, can cross the blood-brain barrier, promote the generation of alpha waves in the brain, and induce relaxation without causing drowsiness. It can also regulate neurotransmitters such as GABA, serotonin, and dopamine, exerting anti-anxiety and anti-stress effects. However, theanine alone has a relatively weak effect and limited bioavailability, making it difficult to meet the needs of people with moderate to severe anxiety.

[0003] In existing technologies, theanine is often combined with ingredients such as γ-aminobutyric acid (GABA), B vitamins, and plant extracts to enhance its anti-anxiety effect. However, the following problems still exist: 1) Poor stability: Theanine and some plant active ingredients (such as flavonoids) are easily degraded by light, heat, and oxidation, affecting the stability of the product; 2) Low bioavailability: The active ingredients (such as hesperidin) in some plant extracts (such as Citrus aurantium extract) are highly lipid-soluble and poorly water-soluble, resulting in low oral absorption; 3) Lack of sustained-release design: Due to the lack of sustained-release design, theanine and other active ingredients are released too quickly in the gastrointestinal tract, resulting in a short duration of action and making it difficult to maintain an anti-anxiety effect. Therefore, there is an urgent need to develop a tea-based theanine composition with good stability, high bioavailability, and sustained-release properties to relieve anxiety and meet practical application needs. Summary of the Invention

[0004] The purpose of this invention is to provide a tea-based theanine composition for relieving anxiety, its preparation method, and its application. The method involves combining tea theanine with a composite carrier to obtain a carrier composite material; encapsulating plant extracts with the carrier composite material in step S1 to obtain an inclusion material; mixing the inclusion material from step S2 with a ternary synergistic complex and compound vitamins, followed by granulation, to obtain the tea-based theanine composition for relieving anxiety. The prepared tea-based theanine composition for relieving anxiety not only possesses good stability, sustained-release properties, and anxiety-relieving effects, but also exhibits good bioavailability and safety, thus comprehensively improving the overall performance of the tea-based theanine composition.

[0005] The technical problem this invention aims to solve is as follows: In the prior art, theanine is usually compounded with ingredients such as γ-aminobutyric acid, B vitamins, and plant extracts to enhance the anti-anxiety effect, but the following problems still exist: 1) Poor stability: Theanine and some plant active ingredients (such as flavonoids) are easily degraded by light, heat, and oxidation, affecting the stability of the product; 2) Low bioavailability: The active ingredients (such as hesperidin) in some plant extracts (such as Citrus aurantium extract) are highly lipid-soluble and poorly water-soluble, resulting in low oral absorption rate; 3) Lack of sustained-release design: Due to the lack of sustained-release design, the active ingredients such as theanine are released too quickly in the gastrointestinal tract, resulting in a short duration of action and making it difficult to maintain the anti-anxiety effect.

[0006] The objective of this invention can be achieved through the following technical solutions:

[0007] A method for preparing a tea-based theanine composition for relieving anxiety includes the following steps:

[0008] S1: Theanine from tea leaves is combined with a composite carrier to obtain a carrier composite material;

[0009] S2: The plant extract is encapsulated using the carrier composite material in step S1 to obtain the encapsulated material;

[0010] S3: After mixing the inclusion material from step S2 with the ternary synergistic complex and compound vitamins, granulation is performed to obtain a tea theanine composition for relieving anxiety.

[0011] Furthermore, step S1 specifically includes:

[0012] Theanine from tea leaves was added to deionized water and stirred until completely dissolved to obtain a theanine solution. The composite carrier was added to deionized water and subjected to ultrasonic treatment to obtain a composite carrier dispersion. The composite carrier dispersion was added to the theanine solution and stirred to react. The pH of the system was then adjusted with sodium hydroxide solution and stirring continued. After the reaction was completed, the mixture was cooled to room temperature and transferred to a dialysis bag for dialyzing with deionized water. Finally, it was freeze-dried to obtain the carrier composite material.

[0013] In the above reaction process, the composite carrier has a hydrophobic cavity, and theanine from tea leaves can partially enter the hydrophobic cavity of the composite carrier. In addition, theanine from tea leaves has carboxyl groups, and the composite carrier has hydroxyl groups. They can be better combined with theanine from tea leaves through hydrogen bonding, and finally, a carrier composite material is obtained.

[0014] Furthermore, the mass ratio of theanine in the tea leaves to deionized water is 1:10-20.

[0015] Furthermore, the mass ratio of the composite carrier to deionized water is 1:20-30.

[0016] Furthermore, the ultrasonic treatment time is 15-25 minutes.

[0017] Furthermore, the mass ratio of the composite carrier dispersion to the theanine solution is 1:1.5.

[0018] Furthermore, the temperature of the stirring reaction is 45-55℃, and the time is 2.5-3.5h.

[0019] Furthermore, the pH of the system is adjusted to 6.3-6.7 using 0.1M sodium hydroxide solution.

[0020] Furthermore, the stirring time is 10-20 minutes.

[0021] Furthermore, the freeze-drying temperature is -50 to -40°C, and the time is 24 hours.

[0022] Furthermore, the preparation method of the composite carrier includes the following steps:

[0023] β-Cyclodextrin was added to deionized water and stirred in a water bath until completely dissolved to obtain a cyclodextrin solution. Arabinocalactan was added to deionized water and stirred to obtain an arabinocalactan solution. The arabinocalactan solution was added to the β-cyclodextrin solution, and then stirred in a water bath for reaction. After the reaction was completed, the mixture was cooled to room temperature and finally spray-dried to obtain the composite carrier.

[0024] Furthermore, the mass ratio of β-cyclodextrin to deionized water is 1.8-2.2:45-55.

[0025] Furthermore, the stirring is carried out in a water bath at a temperature of 60-70°C for a duration of 0.5-1 hour.

[0026] Furthermore, the mass ratio of the arabinogalactan to deionized water is 1.8-2.2:45-55.

[0027] Furthermore, the stirring time is 30-40 minutes, and the temperature is room temperature.

[0028] Furthermore, the mass ratio of the arabinogalactan solution to the β-cyclodextrin solution is 1:1.

[0029] Furthermore, the temperature of the stirring reaction is 60-70℃, and the time is 2-3 hours.

[0030] Furthermore, in the spray drying process, the inlet air temperature is 160-180℃, the outlet air temperature is 80-90℃, and the feed rate is 10mL / min.

[0031] Furthermore, step S2 specifically includes:

[0032] The carrier composite material from step S1 is added to deionized water and stirred to obtain component A. The plant extract is added to anhydrous ethanol and stirred until completely dissolved to obtain component B. Component B is added to component A and stirred to react. After the reaction is complete, the mixture is cooled to room temperature, allowed to stand, filtered, washed with deionized water, and finally vacuum dried to obtain the inclusion material.

[0033] In the above reaction process, the carrier composite material has hydrophobic cavities, which can further encapsulate plant extracts, finally yielding an encapsulated material.

[0034] Furthermore, the mass ratio of the carrier composite material to deionized water is 1:10-15.

[0035] Furthermore, the stirring time is 15-25 minutes, and the temperature is 55-65℃.

[0036] Furthermore, the mass ratio of the plant extract to anhydrous ethanol is 1:5-10.

[0037] Furthermore, the plant extract is composed of a mixture of Citrus aurantium extract and Lilium spp. extract in a mass ratio of 1:2-3.

[0038] Furthermore, the mass ratio of component B to component A is 1:1.

[0039] Furthermore, the stirring reaction time is 2.5-3.5 hours, and the temperature is room temperature.

[0040] Furthermore, step S3 specifically includes:

[0041] The inclusion material, ternary synergistic complex, and compound vitamins from step S2 are mixed, then a binder solution is added, and the mixture is stirred evenly. After granulation, drying, and granulation, an anxiety-relieving tea theanine composition is obtained.

[0042] Furthermore, the mass ratio of the inclusion material, the ternary synergistic complex, and the compound vitamins is 1:0.4-0.5:0.1-0.2.

[0043] Furthermore, the ternary synergistic complex is composed of γ-aminobutyric acid, casein hydrolysate, and rice hydrolysate in a mass ratio of 3:1.8-2.2:0.8-1.2.

[0044] Furthermore, the compound vitamin is composed of vitamin B6 and vitamin B12 mixed in a mass ratio of 2-3:1.

[0045] Furthermore, the adhesive solution is a hydroxypropyl methylcellulose solution.

[0046] An anxiety-relieving tea theanine composition prepared by the method described above.

[0047] The beneficial effects of this invention are:

[0048] (1) In the technical solution of the present invention, a carrier composite material is obtained by combining tea theanine with a composite carrier; the composite carrier and tea theanine are combined through inclusion and hydrogen bond interactions, which enhances the binding force between the two and improves the stability of tea theanine, enhances its water solubility and dispersibility, and allows tea theanine to be slowly hydrolyzed in the gastrointestinal tract to achieve sustained and controlled release, prolonging the duration of drug efficacy. In addition, it can also improve the ability of tea theanine to cross the blood-brain barrier; the composite carrier is prepared by β-cyclodextrin and arabinogalactan, which can not only improve the stability and bioavailability of the tea theanine composition, but also improve its sustained release effect and anti-anxiety effect, and improve its sensory characteristics. In addition, the prepared carrier composite material can also provide hydrophobic cavities for subsequent reactions. Further used for encapsulating plant extracts; the plant extracts are encapsulated using the carrier composite material in step S1 to obtain an encapsulation material; wherein, the plant extracts are composed of a mixture of Citrus aurantium extract and Lilium brevis extract, and the combination of the two has a synergistic effect, which can not only improve the antioxidant properties of the tea theanine composition, but also further improve its anti-anxiety and sedative effects; the encapsulation of plant extracts through the hydrophobic cavities in the carrier composite material can effectively improve the water solubility of plant extracts and enhance their chemical stability, and can further improve the release rate of the tea theanine composition in the gastrointestinal tract, prolong the duration of drug action, and also improve the bioavailability and transmembrane absorption capacity of the tea theanine composition, reduce its irritation to the gastrointestinal tract, and improve the safety and tolerability of administration.

[0049] (2) In the technical solution of the present invention, the tea theanine composition for relieving anxiety is obtained by mixing the inclusion material in step S2 with the ternary synergistic complex and the compound vitamins and then granulating it. The ternary synergistic complex is composed of γ-aminobutyric acid, casein hydrolysate and rice hydrolysate. The three components have a good synergistic effect and can effectively improve the anxiety-relieving performance, stability and bioavailability of the tea theanine composition. γ-aminobutyric acid can produce an immediate sedative effect. Casein hydrolysate can enhance the receptor sensitivity to γ-aminobutyric acid, making its effect stronger and longer-lasting. Rice hydrolysate can produce a mild sedative and comfortable feeling. The combination of the three components can improve the overall anxiety-relieving performance and bioavailability of the tea theanine composition, and enhance the duration and safety of the efficacy. In addition, the compound vitamins are composed of vitamin B6 and vitamin B12, which can also improve the anxiety-relieving performance and stability of the tea theanine composition. The mixing of the inclusion material with the ternary synergistic complex and the compound vitamins improves the overall performance of the tea theanine composition.

[0050] (3) In the technical solution of the present invention, the theanine in tea leaves is combined with a composite carrier, and then the plant extract is encapsulated to obtain an encapsulated material. The encapsulated material is mixed with a ternary synergistic complex and compound vitamins, granulated, and finally an anxiety-relieving tea theanine composition is obtained. The prepared anxiety-relieving tea theanine composition not only has good stability, sustained-release performance and anxiety-relieving performance, but also has good bioavailability and safety, thus improving the overall performance of the tea theanine composition. Detailed Implementation

[0051] The technical solutions of the present invention will be clearly and completely described below with reference to the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the scope of protection of the present invention.

[0052] The specific parameters of the raw materials used in this invention are as follows:

[0053] Tea leaf theanine, batch number: 19111503, provided by Hunan Jinong Bio-resources Co., Ltd.; β-cyclodextrin, CAS number: 7585-39-9, product number: C804562, provided by Shanghai Maclean Biochemical Technology Co., Ltd.; arabinogalactan (food grade arabinogalactan), CAS number: 9036-66-2, product number: A304929, provided by Shanghai Aladdin Biochemical Technology Co., Ltd.; Citrus aurantium extract, specification: 10:1, provided by Shaanxi Bohong Health Industry Co., Ltd.; Lily extract, specification: 10:1, provided by Xi'an Haoze Biotechnology Co., Ltd.; Casein hydrolysate, CAS number: 9000-71-9, provided by Jiangsu Caiwei Biotechnology Co., Ltd.; Rice hydrolyzed protein, provided by Zhengzhou Yuhe Food Additives Co., Ltd.

[0054] Example 1

[0055] The preparation method of the tea theanine composition for relieving anxiety includes the following steps:

[0056] S1: The theanine in tea leaves was added to deionized water at a mass ratio of 1:10 and stirred at room temperature until completely dissolved to obtain a theanine solution. The composite carrier was added to deionized water at a mass ratio of 1:20 and ultrasonically treated for 15 min (ultrasonic power of 100W and ultrasonic frequency of 40kHz) to obtain a composite carrier dispersion. The composite carrier dispersion was added to the theanine solution at a mass ratio of 1:1.5 and stirred in a water bath at 45℃ for 3.5 h. The pH of the system was then adjusted to 6.3 with 0.1M sodium hydroxide solution and stirred for another 10 min. After the reaction was completed, the mixture was cooled to room temperature and transferred to a dialysis bag (molecular weight cutoff of 3500 Da). Dialysis was performed with deionized water for 3 days. Finally, the mixture was freeze-dried at -50℃ for 24 h to obtain the carrier composite material.

[0057] The preparation method of the composite carrier includes the following steps:

[0058] β-Cyclodextrin was added to deionized water at a mass ratio of 1.8:45, and stirred at 600 rpm for 1 hour in a 60°C water bath until completely dissolved to obtain a cyclodextrin solution. Arabinocalactan was added to deionized water at a mass ratio of 1.8:45, and stirred at room temperature for 30 minutes to obtain an arabinocalactan solution. The arabinocalactan solution was added to the β-cyclodextrin solution at a mass ratio of 1:1, and the mixture was stirred at 600 rpm for 3 hours in a 60°C water bath. After the reaction, the mixture was cooled to room temperature and then spray-dried at an inlet air temperature of 160°C, an outlet air temperature of 80°C, and a feed rate of 10 mL / min to obtain the composite carrier.

[0059] S2: According to the mass ratio of carrier composite material to deionized water of 1:10, the carrier composite material from step S1 is added to deionized water and stirred at 55°C for 25 min to obtain component A. According to the mass ratio of plant extract to anhydrous ethanol of 1:5, the plant extract is added to anhydrous ethanol and stirred until completely dissolved to obtain component B. According to the mass ratio of component B to component A of 1:1, component B is added to component A and stirred at room temperature for 2.5 h. After the reaction is completed, it is cooled to room temperature and allowed to stand at 4°C for 12 h. After filtration, it is washed 3 times with deionized water (each time the mass of deionized water is the same as the above deionized water). Finally, it is vacuum dried at 40°C for 12 h to obtain the inclusion material. The plant extract is composed of Citrus aurantium extract and Lilium brevis extract mixed at a mass ratio of 1:2.

[0060] S3: According to the mass ratio of inclusion material, ternary synergistic complex, and compound vitamins in step S2, mix the inclusion material, ternary synergistic complex, and compound vitamins for 15 min, add 5 wt% hydroxypropyl methylcellulose solution (the mass of hydroxypropyl methylcellulose solution is 30% of the mass of inclusion material), stir evenly, granulate through a 16-mesh sieve, dry at 40℃ for 3 h, and then granulate again through a 16-mesh sieve to finally obtain the tea theanine composition for relieving anxiety. Among them, the ternary synergistic complex is composed of γ-aminobutyric acid, casein hydrolysate, and rice hydrolysate in a mass ratio of 3:1.8:0.8; the compound vitamins are composed of vitamin B6 and vitamin B12 in a mass ratio of 2:1.

[0061] Example 2

[0062] The preparation method of the tea theanine composition for relieving anxiety includes the following steps:

[0063] S1: The theanine in tea leaves was added to deionized water at a mass ratio of 1:15 and stirred at room temperature until completely dissolved to obtain a theanine solution. The composite carrier was added to deionized water at a mass ratio of 1:25 and ultrasonically treated for 20 min (ultrasonic power of 100 W and ultrasonic frequency of 40 kHz) to obtain a composite carrier dispersion. The composite carrier dispersion was added to the theanine solution at a mass ratio of 1:1.5 and stirred in a 50°C water bath for 3 h. The pH of the system was then adjusted to 6.5 with 0.1 M sodium hydroxide solution and stirred for another 15 min. After the reaction was completed, the mixture was cooled to room temperature and transferred to a dialysis bag (molecular weight cutoff of 3500 Da). Dialysis was performed with deionized water for 3 days. Finally, the mixture was freeze-dried at -45°C for 24 h to obtain the carrier composite material.

[0064] The preparation method of the composite carrier includes the following steps:

[0065] β-Cyclodextrin was added to deionized water at a mass ratio of 2:50, and stirred at 600 rpm for 0.8 h in a 65°C water bath until completely dissolved to obtain a cyclodextrin solution. Arabinocalactan was added to deionized water at a mass ratio of 2:50, and stirred at room temperature for 35 min to obtain an arabinocalactan solution. Arabinocalactan solution was added to β-cyclodextrin solution at a mass ratio of 1:1, and the mixture was stirred at 600 rpm in a 65°C water bath for 2.5 h. After the reaction, the mixture was cooled to room temperature and then spray-dried at an inlet air temperature of 170°C, an outlet air temperature of 85°C, and a feed rate of 10 mL / min to obtain the composite carrier.

[0066] S2: According to the mass ratio of carrier composite material to deionized water of 1:12, the carrier composite material from step S1 is added to deionized water and stirred at 60°C for 20 min to obtain component A. According to the mass ratio of plant extract to anhydrous ethanol of 1:8, the plant extract is added to anhydrous ethanol and stirred until completely dissolved to obtain component B. According to the mass ratio of component B to component A of 1:1, component B is added to component A and stirred at room temperature for 3 h. After the reaction is completed, it is cooled to room temperature and allowed to stand at 4°C for 12 h. After filtration, it is washed 3 times with deionized water (each time the mass of deionized water is the same as the above deionized water). Finally, it is vacuum dried at 45°C for 12 h to obtain the inclusion material. The plant extract is composed of Citrus aurantium extract and Lilium brevis extract mixed at a mass ratio of 1:2.5.

[0067] S3: According to the mass ratio of inclusion material, ternary synergistic complex, and compound vitamins in step S2, mix the inclusion material, ternary synergistic complex, and compound vitamins for 18 min, add 8 wt% hydroxypropyl methylcellulose solution (the mass of hydroxypropyl methylcellulose solution is 30% of the mass of inclusion material), stir evenly, granulate through an 18-mesh sieve, dry at 45℃ for 2.5 h, and then granulate again through an 18-mesh sieve to finally obtain the tea theanine composition for relieving anxiety. The ternary synergistic complex is composed of γ-aminobutyric acid, casein hydrolysate, and rice hydrolysate in a mass ratio of 3:2:1; the compound vitamins are composed of vitamin B6 and vitamin B12 in a mass ratio of 2.5:1.

[0068] Example 3

[0069] The preparation method of the tea theanine composition for relieving anxiety includes the following steps:

[0070] S1: The theanine in tea leaves was added to deionized water at a mass ratio of 1:20 and stirred at room temperature until completely dissolved to obtain a theanine solution. The composite carrier was added to deionized water at a mass ratio of 1:30 and ultrasonically treated for 25 min (ultrasonic power of 100W and ultrasonic frequency of 40kHz) to obtain a composite carrier dispersion. The composite carrier dispersion was added to the theanine solution at a mass ratio of 1:1.5 and stirred in a water bath at 55℃ for 2.5 h. The pH of the system was then adjusted to 6.7 with 0.1M sodium hydroxide solution and stirred for another 20 min. After the reaction was completed, the mixture was cooled to room temperature and transferred to a dialysis bag (molecular weight cutoff of 3500 Da). Dialysis was performed with deionized water for 3 days. Finally, the mixture was freeze-dried at -40℃ for 24 h to obtain the carrier composite material.

[0071] The preparation method of the composite carrier includes the following steps:

[0072] β-Cyclodextrin was added to deionized water at a mass ratio of 2.2:55, and stirred at 600 rpm for 0.5 h in a 70°C water bath until completely dissolved to obtain a cyclodextrin solution. Arabinocalactan was added to deionized water at a mass ratio of 2.2:55, and stirred at room temperature for 40 min to obtain an arabinocalactan solution. The arabinocalactan solution was added to the β-cyclodextrin solution at a mass ratio of 1:1, and the mixture was stirred at 600 rpm for 2 h in a 70°C water bath. After the reaction, the mixture was cooled to room temperature and then spray-dried. The inlet air temperature was 180°C, the outlet air temperature was 90°C, and the feed rate was 10 mL / min to obtain the composite carrier.

[0073] S2: According to the mass ratio of carrier composite material to deionized water of 1:15, the carrier composite material from step S1 is added to deionized water and stirred at 65°C for 15 min to obtain component A. According to the mass ratio of plant extract to anhydrous ethanol of 1:10, the plant extract is added to anhydrous ethanol and stirred until completely dissolved to obtain component B. According to the mass ratio of component B to component A of 1:1, component B is added to component A and stirred at room temperature for 3.5 h. After the reaction is completed, it is cooled to room temperature and allowed to stand at 4°C for 12 h. After filtration, it is washed 3 times with deionized water (each time the mass of deionized water is the same as the above deionized water). Finally, it is vacuum dried at 50°C for 12 h to obtain the inclusion material. The plant extract is composed of Citrus aurantium extract and Lilium brevis extract mixed at a mass ratio of 1:3.

[0074] S3: According to the mass ratio of inclusion material, ternary synergistic complex, and compound vitamins in step S2, mix the inclusion material, ternary synergistic complex, and compound vitamins for 20 min, add 10 wt% hydroxypropyl methylcellulose solution (the mass of hydroxypropyl methylcellulose solution is 30% of the mass of inclusion material), stir evenly, granulate through a 20-mesh sieve, dry at 50℃ for 2 h, and then granulate again through a 20-mesh sieve to finally obtain the tea theanine composition for relieving anxiety. Among them, the ternary synergistic complex is composed of γ-aminobutyric acid, casein hydrolysate, and rice hydrolysate in a mass ratio of 3:2.2:1.2; the compound vitamins are composed of vitamin B6 and vitamin B12 in a mass ratio of 3:1.

[0075] Comparative Example 1

[0076] The difference between this comparative example and Example 3 is that, in the preparation of the tea theanine composition for relieving anxiety, in step S1, the composite carrier is replaced with β-cyclodextrin by an equal mass, while the remaining steps and raw materials are the same as in Example 3.

[0077] S1: Theanine from tea leaves was added to deionized water at a mass ratio of 1:20 and stirred at room temperature until completely dissolved to obtain a theanine solution. β-cyclodextrin was added to deionized water at a mass ratio of 1:30 and ultrasonically treated for 25 min (ultrasonic power 100 W, ultrasonic frequency 40 kHz) to obtain a cyclodextrin solution. The cyclodextrin solution was added to the theanine solution at a mass ratio of 1:1.5 and stirred in a 55℃ water bath for 2.5 h. The pH of the system was then adjusted to 6.7 with 0.1 M sodium hydroxide solution and stirred for another 20 min. After the reaction was completed, the mixture was cooled to room temperature and transferred to a dialysis bag (molecular weight cutoff 3500 Da). Dialysis with deionized water was performed for 3 days, and finally, the mixture was freeze-dried at -40℃ for 24 h to obtain the carrier composite material.

[0078] Comparative Example 2

[0079] The difference between this comparative example and Example 3 is that, in the preparation of the tea theanine composition for relieving anxiety, in step S1, the composite carrier is replaced by an equal mass of arabinogalactan, while the remaining steps and raw materials are the same as in Example 3.

[0080] S1: The theanine in tea leaves was added to deionized water at a mass ratio of 1:20 and stirred at room temperature until completely dissolved to obtain a theanine solution. The arabinogalactan was added to deionized water at a mass ratio of 1:30 and ultrasonically treated for 25 minutes (ultrasonic power of 100W and ultrasonic frequency of 40kHz) to obtain an arabinogalactan solution. The arabinogalactan solution was added to the theanine solution at a mass ratio of 1:1.5 and stirred at room temperature for 2.5 hours. After the reaction was completed, the mixture was transferred to a dialysis bag (molecular weight cutoff of 3500 Da) and dialyzed with deionized water for 3 days. Finally, it was freeze-dried at -40°C for 24 hours to obtain the carrier composite material.

[0081] Comparative Example 3

[0082] The difference between this comparative example and Example 3 is that, in the preparation of the tea theanine composition for relieving anxiety, in step S2, the plant extract is replaced by the same amount of Citrus aurantium extract, while the remaining steps and raw materials are the same as in Example 3.

[0083] S2: According to the mass ratio of carrier composite material to deionized water of 1:15, add the carrier composite material from step S1 to deionized water and stir at 65°C for 15 min to obtain component A. According to the mass ratio of Citrus aurantium extract to anhydrous ethanol of 1:10, add Citrus aurantium extract to anhydrous ethanol and stir until completely dissolved to obtain component B. According to the mass ratio of component B to component A of 1:1, add component B to component A and stir and react at room temperature for 3.5 h. After the reaction is completed, cool to room temperature and let stand at 4°C for 12 h. After filtration, wash three times with deionized water (each time the mass of deionized water is the same as the above deionized water). Finally, vacuum dry at 50°C for 12 h to obtain the inclusion material.

[0084] Comparative Example 4

[0085] The difference between this comparative example and Example 3 is that, in the preparation of the tea theanine composition for relieving anxiety, in step S2, the plant extract is replaced by lily extract, while the remaining steps and raw materials are the same as in Example 3.

[0086] S2: According to the mass ratio of carrier composite material to deionized water of 1:15, add the carrier composite material from step S1 to deionized water and stir at 65°C for 15 min to obtain component A. According to the mass ratio of lily extract to anhydrous ethanol of 1:10, add lily extract to anhydrous ethanol and stir until completely dissolved to obtain component B. According to the mass ratio of component B to component A of 1:1, add component B to component A and stir at room temperature for 3.5 h. After the reaction is completed, cool to room temperature and stand at 4°C for 12 h. After filtration, wash 3 times with deionized water (each time the mass of deionized water is the same as the above deionized water). Finally, vacuum dry at 50°C for 12 h to obtain the inclusion material.

[0087] Comparative Example 5

[0088] The difference between this comparative example and Example 3 is that, in the preparation of the tea theanine composition for relieving anxiety, in step S3, the ternary synergistic complex is replaced by a binary synergistic complex of equal mass. The ternary synergistic complex is composed of a mixture of γ-aminobutyric acid and casein hydrolysate. The remaining steps and raw materials are the same as in Example 3.

[0089] S3: According to the mass ratio of inclusion material, binary synergistic complex, and compound vitamins of 1:0.5:0.2, mix the inclusion material, ternary synergistic complex, and compound vitamins from step S2 for 20 min, add 10wt% hydroxypropyl methylcellulose solution (the mass of hydroxypropyl methylcellulose solution is 30% of the mass of inclusion material), stir evenly, granulate through a 20-mesh sieve, dry at 50℃ for 2 h, and then granulate again through a 20-mesh sieve to finally obtain the tea theanine composition for relieving anxiety. Among them, the binary synergistic complex is composed of γ-aminobutyric acid and casein hydrolysate mixed in a mass ratio of 3:3.4; the compound vitamins are composed of vitamin B6 and vitamin B12 mixed in a mass ratio of 3:1.

[0090] Comparative Example 6

[0091] The difference between this comparative example and Example 3 is that, in the preparation of the tea theanine composition for relieving anxiety, in step S3, the ternary synergistic complex is replaced by a binary synergistic complex of equal mass. The ternary synergistic complex is composed of a mixture of γ-aminobutyric acid and rice hydrolyzed protein. The remaining steps and raw materials are the same as in Example 3.

[0092] S3: According to the mass ratio of inclusion material, binary synergistic complex, and compound vitamins of 1:0.5:0.2, mix the inclusion material, ternary synergistic complex, and compound vitamins from step S2 for 20 min, add 10wt% hydroxypropyl methylcellulose solution (the mass of hydroxypropyl methylcellulose solution is 30% of the mass of inclusion material), stir evenly, granulate through a 20-mesh sieve, dry at 50℃ for 2 h, and then granulate again through a 20-mesh sieve to finally obtain the tea theanine composition for relieving anxiety. Among them, the binary synergistic complex is composed of γ-aminobutyric acid and rice hydrolyzed protein mixed in a mass ratio of 3:3.4; the compound vitamins are composed of vitamin B6 and vitamin B12 mixed in a mass ratio of 3:1.

[0093] Comparative Example 7

[0094] The difference between this comparative example and Example 3 is that, in the preparation of the tea theanine composition for relieving anxiety, in step S3, the ternary synergistic complex is replaced by a binary synergistic complex of equal mass. The ternary synergistic complex is composed of a mixture of casein hydrolysate and rice hydrolysate. The remaining steps and raw materials are the same as in Example 3.

[0095] S3: According to the mass ratio of inclusion material, binary synergistic complex, and compound vitamins of 1:0.5:0.2, mix the inclusion material, ternary synergistic complex, and compound vitamins from step S2 for 20 min, add 10wt% hydroxypropyl methylcellulose solution (the mass of hydroxypropyl methylcellulose solution is 30% of the mass of inclusion material), stir evenly, granulate through a 20-mesh sieve, dry at 50℃ for 2 h, and then granulate again through a 20-mesh sieve to finally obtain the tea theanine composition for relieving anxiety. Among them, the binary synergistic complex is composed of casein hydrolysate and rice hydrolysate protein mixed in a mass ratio of 3:3.4; the compound vitamins are composed of vitamin B6 and vitamin B12 mixed in a mass ratio of 3:1.

[0096] The anxiety-relieving effects of the tea theanine compositions prepared in Examples 1-3 and Comparative Examples 1-7 were evaluated. The specific experimental methods are as follows:

[0097] 1. Experimental Materials

[0098] (1) Test samples: Theanine-containing tea compositions for relieving anxiety prepared in Examples 1-3 and Comparative Examples 1-7;

[0099] (2) Experimental animals, rearing methods and environment: Wild-type AB strain zebrafish were bred by natural pair mating and reproduction, with an age of 4 days after fertilization (4dpf).

[0100] Zebrafish were raised in aquarium water at 28℃, with 200mg of readily soluble sea salt added per 1L of reverse osmosis water. The conductivity was 480-510μS / cm; pH was 6.9-7.2; hardness was 53.7-71.6mg / L CaCO3; the laboratory animal use license number was SYXK (Zhejiang) 2012-0171; and the husbandry and management met the requirements of the international AAALAC certification.

[0101] (3) Modeling reagent: mCPP (1-(3-chlorophenyl)piperazine hydrochloride), which was prepared into a stock solution with a concentration of 23.6 mg / mL using ultrapure water before use, and the working solution concentration was 23.6 μg / mL;

[0102] (4) Positive control reagent: Selegiline, prepared as a 2.5 mg / mL stock solution with DMSO before use, and the working solution concentration is 25 μg / mL.

[0103] 2. Experimental Methods

[0104] (1) Establishing a zebrafish anxiety model:

[0105] Modeling group: 4-day-fever zebrafish were randomly selected and treated with 23.6 μg / mL mCPP aqueous solution for 24 hours via water-soluble exposure to establish a zebrafish anxiety model. This group was then further divided into a model control group, a positive control group, and each test sample group.

[0106] Normal control group: treated with the same volume of fish farming water during the same period;

[0107] After treatment, behavioral or biochemical indicators confirmed that the zebrafish in the model group showed an increase in total movement distance, indicating that the zebrafish anxiety model was successfully established.

[0108] (2) Grouping and drug administration are as follows:

[0109] Model control group: 30 zebrafish were given 3 mL of fish tank water in the model control group;

[0110] Positive control group: 30 zebrafish in the positive control group were given 3 mL of 25 μg / mL selegiline solution;

[0111] Test sample group: divided into example group and comparative example group;

[0112] Example group: 90 zebrafish were randomly divided into 3 groups of 30 each, and exposed to 3 mL of 1000 μg / mL aqueous solution of the tea theanine composition prepared in Examples 1-3.

[0113] Comparative control group: 210 zebrafish were randomly divided into 7 groups of 30 each, and exposed to 3 mL of 1000 μg / mL aqueous solution of tea theanine composition prepared in comparative controls 1-7.

[0114] Normal control group: consisting of 30 zebrafish that have not undergone modeling, and given 3 mL of fish tank water at the same time.

[0115] After treatment at 28℃ for 24 hours, the total movement distance (S1) of zebrafish within 1 hour was measured using a behavior analyzer. The statistical analysis results of the total movement distance were used to evaluate the anxiety-relieving effect of the tea theanine composition on the anxiety model zebrafish.

[0116] (3) Calculation formula:

[0117] Anxiety-relieving effect (%) = [S1 (model control group) - S1 (test sample group)] / S1 (model control group) × 100%; where S1 is the total distance moved by zebrafish within 1 hour. Experimental data were analyzed using Graph software. Statistical analysis was performed using ANOVA and Dunnett's t-test; p < 0.05 indicated a significant difference.

[0118] (4) Experimental results:

[0119] The test results are shown in Table 1.

[0120] Table 1 Evaluation of the anxiety-relieving effect of tea-theanine composition on zebrafish

[0121]

[0122] Note: Compared with the model control group, *p<0.05, **p<0.01, ***p<0.001.

[0123] As shown in Table 1, comparing Comparative Examples 1-4 and Example 3, the test results of the tea theanine composition for relieving anxiety were worse than those of Example 3 when the composite carrier was replaced by β-cyclodextrin or arabinogalactan in step S1, or when the plant extract was replaced by Citrus aurantium extract or lily extract in step S2. This indicates that the composite carrier prepared by β-cyclodextrin and arabinogalactan, when combined with tea theanine, not only improved the stability, water solubility, and dispersibility of tea theanine, but also improved the bioavailability and sustained-release performance of the tea theanine composition. The plant extract composed of Citrus aurantium extract and lily extract had a synergistic effect, which could better improve the stability and bioavailability of the tea theanine composition, and further enhance the anxiety-relieving performance of the tea theanine composition.

[0124] Comparing Comparative Examples 5-7 with Example 3, it can be seen that in step S3, the binary synergistic complex is composed of a mixture of γ-aminobutyric acid (GABA) and casein hydrolysate, or a mixture of γ-aminobutyric acid and rice hydrolysate, or a mixture of casein hydrolysate and rice hydrolysate. The test results of the final tea theanine composition for relieving anxiety are worse than those of Example 3. This indicates that the ternary synergistic complex composed of γ-aminobutyric acid, casein hydrolysate, and rice hydrolysate can play a better synergistic role, effectively improving the stability, sustained-release performance, and bioavailability of the tea theanine composition, and further enhancing the anxiety-relieving performance of the tea theanine composition.

[0125] As shown in Table 1, the anxiety-relieving tea theanine compositions prepared in Examples 1-3, compared to those prepared in Comparative Examples 1-7, achieved the desired results by combining tea theanine with a composite carrier, then encapsulating plant extracts to obtain an encapsulated material. This encapsulated material was then mixed with a ternary synergistic complex and compound vitamins, granulated, and finally yielded the anxiety-relieving tea theanine compositions. The tea theanine compositions prepared in Comparative Examples 1-7, however, did not meet the performance requirements. This indicates that the anxiety-relieving tea theanine compositions prepared in this invention not only possess good anxiety-relieving properties, stability, and sustained-release properties, but also good bioavailability and safety, demonstrating excellent overall performance.

[0126] In the description of this specification, the references to terms such as "an embodiment," "example," "specific example," etc., indicate that a specific feature, structure, material, or characteristic described in connection with that embodiment or example is included in at least one embodiment or example of the present invention. In this specification, the illustrative expressions of the above terms do not necessarily refer to the same embodiment or example. Furthermore, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in one or more embodiments or examples.

[0127] The above description is merely an example and illustration of the present invention. Those skilled in the art can make various modifications or additions to the specific embodiments described or use similar methods to replace them, as long as they do not deviate from the invention or exceed the scope defined by the present invention, they should all fall within the protection scope of the present invention.

Claims

1. A method for preparing a tea-based theanine composition for relieving anxiety, characterized in that, Includes the following steps: S1: Theanine from tea leaves is combined with a composite carrier to obtain a carrier composite material; S2: The plant extract is encapsulated using the carrier composite material in step S1 to obtain the encapsulated material; S3: After mixing the inclusion material from step S2 with the ternary synergistic complex and compound vitamins, granulation is performed to obtain a tea theanine composition for relieving anxiety. Step S1 is as follows: Theanine from tea leaves was added to deionized water and stirred until completely dissolved to obtain a theanine solution. The composite carrier was added to deionized water and subjected to ultrasonic treatment to obtain a composite carrier dispersion. The composite carrier dispersion was added to the theanine solution and stirred to react. The pH of the system was then adjusted with sodium hydroxide solution and stirring continued. After the reaction was completed, the mixture was cooled to room temperature and transferred to a dialysis bag for dialyzing with deionized water. Finally, it was freeze-dried to obtain the carrier composite material. The preparation method of the composite carrier includes the following steps: β-Cyclodextrin was added to deionized water and stirred in a water bath until completely dissolved to obtain a cyclodextrin solution. Arabinocalactan was added to deionized water and stirred to obtain an arabinocalactan solution. The arabinocalactan solution was added to the β-cyclodextrin solution and then stirred in a water bath. After the reaction was completed, the mixture was cooled to room temperature and finally spray-dried to obtain the composite carrier. The plant extract is composed of a mixture of immature bitter orange extract and lily extract in a mass ratio of 1:2-3; The ternary synergistic complex is composed of γ-aminobutyric acid, casein hydrolysate, and rice hydrolysate in a mass ratio of 3:1.8-2.2:0.8-1.

2.

2. The method for preparing a tea-based theanine composition for relieving anxiety according to claim 1, characterized in that, Step S2 is as follows: The carrier composite material from step S1 was added to deionized water and stirred to obtain component A. The plant extract was added to anhydrous ethanol and stirred until completely dissolved to obtain component B. Component B was added to component A and stirred to react. After the reaction was completed, the mixture was cooled to room temperature, allowed to stand, filtered, washed with deionized water, and finally vacuum dried to obtain the inclusion material.

3. The method for preparing a tea-based theanine composition for relieving anxiety according to claim 1, characterized in that, Step S3 is as follows: The inclusion material, ternary synergistic complex, and compound vitamins from step S2 are mixed, then a binder solution is added, and the mixture is stirred evenly. After granulation, drying, and granulation, an anxiety-relieving tea theanine composition is obtained.

4. The method for preparing a tea-based theanine composition for relieving anxiety according to claim 3, characterized in that, The compound vitamin is composed of vitamin B6 and vitamin B12 mixed in a mass ratio of 2-3:

1.

5. An anxiety-relieving tea theanine composition prepared by the method according to any one of claims 1-4.

6. The use of the tea theanine composition for relieving anxiety as described in claim 5 in the preparation of functional foods or health foods for relieving anxiety.

Images