Insecticide composition for effectively preventing and treating cotton aphid and application

Abstract

The invention relates to the technical field of pesticides, in particular to an insecticide composition for effectively preventing and treating cotton aphid and application. The pesticide composition comprises an active component A and an active component B, the active ingredient A is flufenacil; the active ingredient B is carbosulfan or sulfoxaflor; the mass ratio of the active component A to the active component B is 1: (2-8) or (2-8): 1. The pesticide composition with a synergistic effect in the field of cotton aphid prevention and control is obtained through research, has a remarkable higher prevention and control effect on cotton aphids when being compounded according to a specific proportion, is suitable for cotton aphid prevention and control of various cotton aphid harmful plants, has the characteristics of high efficiency, low toxicity and sustainability, and has an important application value.

Description

Technical Field

[0001] This invention relates to the field of pesticide technology, and in particular to an effective insecticide composition and application for controlling cotton aphids. Background Technology

[0002] Cotton is an important economic crop, but the occurrence of pests and diseases during cotton production is becoming increasingly serious, severely threatening cotton yield and quality and affecting farmers' economic income. Among the many cotton pests, the cotton aphid (… Aphid gossip The cotton aphid (Glover), a common and highly prolific piercing-sucking pest, causes particularly severe damage. It can occur throughout the entire cotton growing season, using its stylet to pierce plant tissues and suck sap, resulting in slow growth, leaf curling, and even leaf drop. Simultaneously, its honeydew secretions can lead to sooty mold, affecting photosynthesis and consequently hindering normal cotton development. Furthermore, the cotton aphid has a very high reproductive capacity, and its population can rapidly increase in a short period. Its strong migratory ability allows it to spread quickly in the field, significantly increasing the difficulty of control.

[0003] Chemical control has always been the primary method for managing cotton aphids. However, the problem lies in the fact that cotton aphids have developed varying degrees of resistance to many insecticides, including but not limited to organophosphates, carbamates, and pyrethroids. This has significantly reduced the effectiveness of single chemical pesticides, resulting in unstable control and failing to meet the demands of efficient and sustainable agriculture. Moreover, long-term use of these pesticides alone can easily induce pesticide resistance in pests and may also cause ecological and environmental problems, such as the simultaneous killing of natural enemy insects, thereby damaging biodiversity and leading to a resurgence of pests. Therefore, developing insecticide combinations with novel mechanisms of action, synergistic effects, and low risk of resistance has become an important direction in modern plant protection research.

[0004] Dimpropyridaz is a type of monoamide insecticide. It acts on the TRP channel of the insect stringing organ, acting as a TRPV channel inhibitor. As a prodrug, dimpropyridaz inhibits stringing organ neuronal function and reduces intracellular calcium levels in target pests through hydrolysis or enzymatic metabolism and activation. 2+Concentration. Feeding by target pests is completely inhibited within tens of minutes to several hours, gradually leading to paralysis, dehydration, and wilting, eventually resulting in starvation and death after a few days. Acetaminophen has strong systemic properties, allowing for rapid absorption by crops after application and rapid translocation within the plant. It provides good control of pests affecting roots, stems, and leaves. It can be used on fruit trees, vegetables, legumes, cotton, grains, potatoes, as well as flowers and ornamental plants to control pests of Lepidoptera, Coleoptera, Diptera, Hemiptera, Thysanoptera, and Isoptera, especially effective against piercing-sucking pests such as aphids, whiteflies, and psyllids. It is also friendly to soil and aquatic organisms and harmless to beneficial insects such as pollinators and birds. Acetaminophen can quickly stop pest feeding, reduce plant nutrient loss, prevent disease spread, promote healthy plants, and improve crop yield and quality.

[0005] Sulfpoxaflor is a novel neonicotinoid insecticide with a unique sulfoxide imide group, hence it is also known as a sulfonamide insecticide. Its mechanism of action involves activating a unique binding site within the neonicotinic acetylcholine receptor (nAChR) in the insect's nervous system, thereby affecting the normal transmission of nerve signals. Although flupyradifurone has only been used for a short time, cotton aphids have already developed varying degrees of resistance to it, and this resistance is gradually increasing.

[0006] Carbosulfan is a carbamate insecticide that acts on acetylcholinesterase (AChE) in insects. It is an acetylcholinesterase inhibitor, and its mechanism of action primarily involves interfering with the insect's nervous system and inhibiting cholinesterase activity, causing sustained excitation of the insect's muscles and glands, ultimately leading to death. Carbosulfan has played an important role in the early control of cotton aphids in many regions; however, resistance to carbosulfan in cotton aphids is also gradually increasing, thus limiting its application. Summary of the Invention

[0007] To address the problems existing in the prior art, the present invention provides an effective insecticide composition and application for controlling cotton aphids.

[0008] In a first aspect, the present invention provides an insecticide composition comprising: active ingredient A and active ingredient B; The active ingredient A is acetamiprid; the active ingredient B is thiamethoxam or flonicamid. The mass ratio of active ingredient A to active ingredient B is 1:(2~8) or (2~8):1.

[0009] To address the problem of pesticide resistance in cotton aphid control, this invention proposes a novel binary insecticide combination based on acetamiprid, compounded with traditional insecticide components such as carbofuran or flonicamid. This combination, through the structural differences and synergistic effects of its different molecules, effectively enhances the control of cotton aphids, with low cross-resistance. Furthermore, the insecticide composition provided by this invention effectively reduces the risk of resistance while minimizing negative impacts on non-target organisms and the environment. Optimized pesticide use enables precise and efficient cotton aphid control, ensuring high-yield, high-efficiency, and sustainable cotton production.

[0010] Furthermore, the mass ratio of active ingredient A to active ingredient B is 1:(3~7) or (3~7):1.

[0011] Furthermore, when the active ingredient B is thiamethoxam, the mass ratio of the active ingredient A to the active ingredient B is 1:(4.5~5.5) or (4.5~5.5):1; when the active ingredient B is flonicamid, the mass ratio of the active ingredient A to the active ingredient B is 1:(4.5~5.5) or (4.5~5.5):1.

[0012] Furthermore, the insecticide composition further includes: pesticide-acceptable adjuvants, and is formulated as an emulsifiable concentrate, suspension concentrate, water-dispersible granule, wettable powder, or microcapsule suspension; Preferably, the pesticide is an acceptable adjuvant that is acetone, and the insecticide composition is a solution of acetone.

[0013] Further, the solution comprises: 0.001-1000 g / L chlorfenapyr and 0.005-5000 g / L carbofuran; or, 0.005-5000 g / L chlorfenapyr and 0.001-1000 g / L carbofuran; or, 0.001-1000 g / L pyraclostrobin and 0.005-5000 g / L flonicamid; or, 0.005-5000 g / L pyraclostrobin and 0.001-1000 g / L flonicamid.

[0014] Furthermore, the insecticide composition is an insecticide composition for controlling cotton aphids.

[0015] Secondly, the present invention provides the application of the aforementioned insecticide composition in the control of piercing-sucking pests.

[0016] Furthermore, the piercing-sucking pest is an aphid; Preferably, the piercing-sucking pest is the cotton aphid.

[0017] Thirdly, the present invention provides a method for controlling piercing-sucking pests, comprising: applying the aforementioned insecticide composition at an effective dose to the piercing-sucking pest, its growth environment, or plants requiring protection.

[0018] Further, the plant includes one or more of the following: plants of the genera *Cotton*, *Cucumber*, *Capsicum*, *Solanum*, *Watermelon*, *Cucumber*, *Citrus*, or *Chrysanthemum*; preferably, the plant is cotton.

[0019] The insecticide composition provided by this invention is particularly suitable for the control of cotton aphids on cotton, possibly because the insecticide composition is more suitable for the ecological environment of cotton and has more precise targeting for cotton aphid biotypes that use cotton as a host.

[0020] The present invention has the following beneficial effects: This invention addresses the practical problems currently faced in agricultural pest control by developing an insecticide composition specifically targeting the cotton aphid, a piercing-sucking pest in cotton crops. Through a combination of acetamiprid with either carbofuran or flonicamid in specific ratios, it achieves a significantly high killing effect against cotton aphids. The composition is highly targeted, effective, and exhibits stable synergistic effects, demonstrating promising application prospects and significant industrialization value. It fills a technological gap in existing cotton aphid control combinations and provides a more efficient, safer, and more environmentally friendly solution for agricultural pest and disease control. Detailed Implementation

[0021] To make the objectives, technical solutions, and advantages of this invention clearer, the technical solutions of this invention will be clearly and completely described below. Obviously, the described embodiments are only some embodiments of this invention, not all embodiments. Based on the embodiments of this invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of this invention.

[0022] Unless otherwise specified, the experimental methods involved in the following embodiments are conventional methods in the art. For example, you can refer to the experimental manual in the art or follow the conditions recommended in the manufacturer's instructions.

[0023] Unless otherwise specified, all experimental materials and reagents used in the following examples are commercially available.

[0024] The cotton aphid samples in the following examples were collected from a cotton aphid population in Jinghe cotton field, Xinjiang, China. This population was reared on non-GMO upland cotton seedlings in our laboratory under the following conditions: 20-23 ℃, 60% relative humidity, and a photoperiod of 16:8 h (light:dark).

[0025] Example 1 In order to study the toxicity of chlorfenapyr, carbofuran and their combination to cotton aphid (Aphis gossypii Glover) population, the present invention uses the leaf immersion method to conduct experiments and determine the toxicity and co-toxicity coefficient of the above agents when used alone and in combination.

[0026] 1. Preparation of pharmaceutical formulation First, thiocarbamate, chlorfenapyr, and their compound combinations were prepared at different mass ratios, with a stock solution concentration of 10,000 μg / mL. The specific preparation methods are as follows: thiocarbamate and chlorfenapyr were prepared separately. For the mixture of thiocarbamate and chlorfenapyr, the technical grade drugs were mixed at mass ratios of 1:1, 5:1, and 1:5, and then dissolved in acetone to prepare a stock solution of 10,000 μg / mL. All stock solutions should be stored in a refrigerator at 4°C to prevent the active ingredients from becoming ineffective.

[0027] 2. Bioassay Using a 0.05% Triton X-100 aqueous solution as the solvent, the different stock solutions were diluted to create 5 to 7 test concentration gradients. Leaf discs were prepared from cotton true leaves using a perforator (20-21 mm in diameter). The prepared leaf discs were immersed in the solutions of different concentration gradients for 15 seconds, and then air-dried with the back facing up. Each concentration group was tested in triplicate. The control group was treated with a 0.05% Triton X-100 aqueous solution. 2.5 mL of 1.5% agar was added to each well of a 12-well cell culture plate. After the agar had slightly solidified, the leaf discs soaked in the solution were placed on the agar with the back facing up, ensuring full contact between the leaf discs and the agar. Wingless adult aphids of similar size were selected and reared in 12-well cell culture plates, approximately 30 aphids per well. Each concentration group was tested in triplicate. Adequate air circulation was ensured in the incubator; therefore, the culture plates were covered with Xuan paper to prevent cotton aphids from escaping. Each well was sealed with solid glue to ensure the experiment was airtight. The experimental temperature was set at (23±1)℃, the relative humidity was maintained at 60%, and the light:dark cycle was set at 16 hours:8 hours to ensure the environmental conditions required for cotton aphid growth. The rearing time for thiamethoxam, chlorfenapyr, and various compound insecticide combinations was 72 hours.

[0028] 3. Mortality assessment and statistical analysis After the experiment, the cotton aphids were gently touched with a paintbrush. If the aphids did not react, or only a few appendages such as antennae or legs showed weak movement, they were considered dead. Based on this standard, the number of dead and surviving aphids in each culture well was recorded. PoloPlus 2.0 software was used to analyze the experimental data and calculate the toxicity regression parameters for each group of pesticides. The main calculated parameters included: slope (and its standard error), LC...50 (median lethal concentration, with 95% confidence interval), chi-square value (χ²), and degrees of freedom (df).

[0029] 4. Determination of the efficacy of compound insecticide formulations In the experiment, one of the single-dose reagents was used as the standard reagent, and the LC ratios of the single-dose and mixture were calculated. 50 The toxicity index and co-toxicity coefficient are calculated using the following formulas: Toxicity Index (TI) = LC of the standard reagent 50 LC-10 of the test reagent 50 ×100.

[0030] Similarly, the theoretical toxicity index (TTI) and actual toxicity index (ATI) of the mixture are calculated using the following formulas: Actual toxicity index (ATI) of the mixture = LC of the standard formulation 50 LC of Mixture M 50 ×100.

[0031] The theoretical toxicity index (TTI) of the mixture is calculated as follows: (TI of agent A × A%) + (TI of agent B × B%).

[0032] Finally, the co-toxicity coefficient (CTC) of the mixture is calculated using the formula: CTC = ATI / TTI × 100.

[0033] Co-toxicity coefficient (CTC) is used to assess the synergistic effect of compound drugs. Based on the magnitude of the CTC, the type of action of the compound drug can be determined.

[0034] Evaluation criteria: The following criteria are generally used in this field to evaluate the type of action of drug combination: CTC>120 indicates synergistic effect; CTC = 80~120 indicates additive effect; CTC<80 indicates antagonistic effect.

[0035] 5. Experimental Data and Results The experimental results below demonstrate that the binary compound combination implemented in this study exhibited a significant synergistic effect in controlling cotton aphids, as specifically shown below: Table 1. Cotoxicity coefficients of each combination

[0036] The experimental results show that when chlorfenapyr and carbofuran are combined in ratios of 1:5 and 5:1, the co-toxicity coefficients (CTCs) of the combined mixtures are 780.16 and 1382.38, respectively, both significantly greater than 120, indicating a clear synergistic effect. However, when chlorfenapyr and carbofuran are combined in a 1:1 ratio, the CTC of the combined mixture is less than 80, indicating an antagonistic effect.

[0037] Example 2 In order to study the toxicity of acetamiprid, flonicamid and their combination to cotton aphid (Aphis gossypii Glover) population, this invention uses the leaf dipping method to conduct experiments and determine the toxicity and co-toxicity coefficient of the above agents when used alone and in combination.

[0038] 1. Preparation of pharmaceutical formulation First, flupyradifurone, chlorpyrifos, and their compound combinations were prepared at different mass ratios to obtain a stock solution concentration of 10,000 μg / mL. The specific preparation methods are as follows: flupyradifurone and chlorpyrifos were prepared separately. The technical grade flupyradifurone and chlorpyrifos were mixed separately at mass ratios of 1:1, 5:1, and 1:5, and then dissolved in acetone to prepare a stock solution of 10,000 μg / mL. All stock solutions should be stored in a refrigerator at 4°C to prevent the active ingredients from becoming ineffective.

[0039] 2. Bioassay Using a 0.05% Triton X-100 aqueous solution as the solvent, the different stock solutions were diluted to create 5 to 7 test concentration gradients. Leaf discs were prepared from cotton true leaves using a perforator (20-21 mm in diameter). The prepared leaf discs were immersed in the solutions of different concentration gradients for 15 seconds, and then air-dried with the back facing up. Each concentration group was tested in triplicate. The control group was treated with a 0.05% Triton X-100 aqueous solution. 2.5 mL of 1.5% agar was added to each well of a 12-well cell culture plate. After the agar had slightly solidified, the leaf discs soaked in the solution were placed on the agar with the back facing up, ensuring full contact between the leaf discs and the agar. Wingless adult aphids of similar size were selected and reared in 12-well cell culture plates, approximately 30 aphids per well. Each concentration group was tested in triplicate. Adequate air circulation was ensured in the incubator; therefore, the culture plates were covered with Xuan paper to prevent cotton aphids from escaping. Each well was sealed with solid glue to ensure the experiment was airtight. The experimental temperature was set at (23±1)℃, the relative humidity was maintained at 60%, and the light:dark cycle was set at 16 hours:8 hours to ensure the environmental conditions required for cotton aphid growth. The rearing time for flonicamid, cypermethrin, and each compound insecticide combination was 72 hours.

[0040] 3. Mortality assessment and statistical analysis After the experiment, the cotton aphids were gently touched with a paintbrush. If the aphids did not react, or only a few appendages such as antennae or legs showed weak movement, they were considered dead. Based on this standard, the number of dead and surviving aphids in each culture well was recorded. PoloPlus 2.0 software was used to analyze the experimental data and calculate the toxicity regression parameters for each group of pesticides. The main calculated parameters included: slope (and its standard error), LC... 50(median lethal concentration, with 95% confidence interval), chi-square value (χ²), and degrees of freedom (df).

[0041] 4. Determination of the efficacy of compound insecticide formulations In the experiment, one of the single-dose reagents was used as the standard reagent, and the LC ratios of the single-dose and mixture were calculated. 50 The toxicity index and co-toxicity coefficient are calculated using the following formulas: Toxicity Index (TI) = LC of the standard reagent 50 LC-10 of the test reagent 50 ×100.

[0042] Similarly, the theoretical toxicity index (TTI) and actual toxicity index (ATI) of the mixture are calculated using the following formulas: Actual toxicity index (ATI) of the mixture = LC of the standard formulation 50 LC of Mixture M 50 ×100.

[0043] The theoretical toxicity index (TTI) of the mixture is calculated as follows: (TI of agent A × A%) + (TI of agent B × B%).

[0044] Finally, the co-toxicity coefficient (CTC) of the mixture is calculated using the formula: CTC = ATI / TTI × 100.

[0045] Co-toxicity coefficient (CTC) is used to assess the synergistic effect of compound drugs. Based on the magnitude of the CTC, the type of action of the compound drug can be determined.

[0046] Evaluation criteria: Domestic scholars generally use the following criteria to evaluate the type of action of drug combination: CTC>120 indicates synergistic effect; CTC = 80~120 indicates additive effect; CTC<80 indicates antagonistic effect.

[0047] 5. Experimental Data and Results Based on the experimental results, the following are the experimental data for each drug and its combination: Table 2. Cotoxicity coefficients of each combination

[0048] According to the experimental results, the co-toxicity coefficients (CTCs) of the two compound combinations of chlorfenapyr and flonicamid at a ratio of 1:5 and 5:1 were 1809.17 and 134.21, respectively, both greater than 120, indicating a significant synergistic effect. In particular, the 1:5 ratio of chlorfenapyr to flonicamid showed the best synergistic effect in the experiment, with a CTC of 1809.17.

[0049] The conclusions are as follows: Through the two experimental trials above, the combination of acetamiprid with carbofuran or flonicamid of the present invention, at specific ratios, exhibits a significant synergistic effect in controlling cotton aphids, which are the host plant. This combination not only improves insecticidal efficiency and reduces pesticide dosage, but also effectively delays the development of resistance, demonstrating broad application prospects and practical significance.

[0050] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, and not to limit them; although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some of the technical features; and these modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. An insecticide composition, characterized by, comprising: active ingredient A and active ingredient B; said active ingredient A is xyliamide; said active ingredient B is carbosulfan or sulfoxaflor; the mass ratio of said active ingredient A and active ingredient B is 1: (2-8) or (2-8):

1.

2. The insecticide composition according to claim 1, characterized in that, the mass ratio of said active ingredient A and active ingredient B is 1: (3-7) or (3-7):

1.

3. The insecticide composition according to any one of claims 1-2, characterized in that, when said active ingredient B is carbosulfan, the mass ratio of said active ingredient A and active ingredient B is 1: (4.5-5.5) or (4.5-5.5): 1; when said active ingredient B is sulfoxaflor, the mass ratio of said active ingredient A and active ingredient B is 1: (4.5-5.5) or (4.5-5.5):

1.

4. The insecticide composition according to any one of claims 1 to 3, characterized in that, said insecticide composition further comprises: a pesticide-acceptable adjuvant, and is configured as an emulsifiable concentrate, a suspension concentrate, a water dispersible granule, a wettable powder, or a microcapsule suspension concentrate.

5. The insecticide composition according to claim 4, wherein in said solution, comprising: 0.001-1000 g / L of xyliamide and 0.005-5000 g / L of carbosulfan; or, 0.005-5000 g / L of xyliamide and 0.001-1000 g / L of carbosulfan; or, 0.001-1000 g / L of xyliamide and 0.005-5000 g / L of sulfoxaflor; or, 0.005-5000 g / L of xyliamide and 0.001-1000 g / L of sulfoxaflor.

6. Use of the insecticide composition of any one of claims 1-5 in the control of piercing-sucking pests.

7. Use according to claim 6, characterized in that, said piercing-sucking pests are aphid pests; preferably, said piercing-sucking pests are cotton aphids.

8. A method for controlling a piercing-sucking pest, characterized by, comprising: applying the insecticide composition of any one of claims 1-5 to said piercing-sucking pests, their growing environment, or a plant in need of protection, in an effective amount.

9. The method of claim 8, wherein, said plant is one or more of: a Gossypium plant, a Cucumis plant, a Capsicum plant, a Solanum plant, a Citrullus plant, a Cucurbita plant, a Citrus plant, or a Dendranthema plant; preferably, said plant is cotton.

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