Application of chaetomium to preparation of medicines, health care products and cosmetics for resisting skin injury

Abstract

The invention discloses application of chaetomium to preparation of medicines, health care products and cosmetics for resisting skin injury. Through skin injury resisting activity analysis of the chaetomium cuspidatum, it is verified that the chaetomium cuspidatum can have a good skin injury resisting effect, and when the chaetomium cuspidatum is applied to medicine, health care products and cosmetics, the skin injury can be reduced, and the repairing effect is achieved.

Description

Technical Field

[0001] This invention relates to a novel application of chamomile amine, particularly its application in the preparation of drugs, health products, and cosmetics for treating skin damage. Background Technology

[0002] Fungal natural products, as a treasure trove of new cosmetic raw materials, have significant scientific and commercial value in their development. They help address existing raw material bottlenecks, promote the sustainable development of the cosmetic industry, and overcome the limitations of terrestrial resources: Terrestrial biological resources are increasingly depleted, while the diverse species and abundant metabolites of fungi provide ample space for new raw material development. Simultaneously, they can meet consumers' demands for natural, safe, and effective products: Fungal natural products are characterized by novel structures, diverse activities, and high safety, meeting modern consumers' expectations for cosmetic raw materials. They also promote the development of green cosmetics: Marine microbial cultivation conditions are mild and controllable, and the production process is environmentally friendly, aligning with the green trend in the cosmetic industry. With the arrival of the "efficacy skincare" 2.0 era, consumer demand for natural skincare products continues to rise. Led by bio-fermented skincare products, the skincare industry has formed a raw material trend guided by "natural, green, safe, and effective." For example, ergothioneine was initially discovered in the fungus *Claviceps purpurea*. It is a natural crystalline sulfur-containing amino acid, water-soluble, and renowned in the cosmetic raw material circle for its excellent antioxidant and anti-UV properties.

[0003] Chaetominine was first isolated in 2006 from a marine mangrove endophytic fungus, *Chaetomium* sp. (SZ-2006). Subsequent studies have also found this compound in other species of *Chaetomium* fungi. Chaetominine possesses a unique alkaloid structure. Its structural feature is a rare 6 / 5 / 6 / 5 / 6 pentacyclic system, incorporating multiple heterocyclic units such as indole, quinazolinone, and piperazineone. Chemical structure: Molecular formula: C 22 H 18 N4O4. Structural characteristics: The core of chaetominine is a highly complex polycyclic alkaloid skeleton, containing: an indole fragment (derived from tryptophan); a quinazolinone fragment (derived from anthranilic acid); a piperazine ring (connecting the quinazolinone and another aromatic unit); and an (R)-phenyllactic acid unit (connected via an ester bond). A thorough literature review found no literature on the application of chaetominine in pharmaceuticals and cosmetics for treating skin damage and skin care. Summary of the Invention

[0004] The purpose of this invention is to provide an application of chamomile amine in the preparation of drugs, health products, and cosmetics for treating skin damage. Through research, this invention has discovered that chamomile amine possesses good anti-UV damage repair activity, and its application in drugs, health products, and cosmetics can reduce skin damage and play a repairing role.

[0005] The technical solution of the present invention: the application of chamomile amine in the preparation of anti-skin damage cosmetics.

[0006] In the aforementioned application of chamomile amine in the preparation of anti-skin damage cosmetics, the skin damage includes skin damage caused by ultraviolet radiation and related complications.

[0007] In the aforementioned application of chamomile amine in the preparation of anti-skin damage cosmetics, the cosmetics are skin repair skin care products or cosmetics.

[0008] Application of a type of chamomile amine in the preparation of anti-skin lesion drugs.

[0009] In the aforementioned application of chamomile amine in the preparation of anti-skin damage drugs, the skin damage includes skin damage caused by ultraviolet radiation and related complications.

[0010] In the aforementioned application of chamomile amine in the preparation of anti-skin lesion drugs, the drug types include injections, infusions, powder injections, granules, tablets, granules, powders, oral liquids, sugar-coated tablets, film-coated tablets, enteric-coated tablets, lozenges, granules, pills, ointments, pills, sprays, drop pills, disintegrants, orally disintegrating tablets, and microcapsules, and the drug includes chamomile amine and a drug-acceptable carrier.

[0011] Application of a type of chamomile amine in the preparation of health products for skin damage.

[0012] In the aforementioned application of chamomile amine in the preparation of anti-skin damage health products, the skin damage includes skin damage caused by ultraviolet radiation and related complications.

[0013] In the aforementioned application of chamomile amine in the preparation of anti-skin damage health products, the health products mentioned are those for the prevention of skin damage.

[0014] The aforementioned application of chamomile amine in the preparation of anti-skin damage health products includes chamomile amine and an acceptable carrier for the health product.

[0015] Compared with the prior art, the beneficial effects of the present invention are as follows: This invention provides the application of chamomile amine in the preparation of drugs, health products and cosmetics for the treatment of skin damage. Through the analysis of the anti-skin damage activity of chamomile amine, this invention verifies that chamomile amine can play a good role in the treatment of skin damage. When applied to drugs, health products and cosmetics, it can reduce skin damage and play a repair role. Attached Figure Description

[0016] Figure 1 This is a representative zebrafish photograph from the experimental analysis of the anti-skin damage activity of the present invention, *Trichoderma harzianum*. Detailed Implementation

[0017] The present invention will be further described below with reference to the accompanying drawings and embodiments, but this should not be construed as limiting the invention. The applicant has conducted a series of experiments, which demonstrate that chaetominine can effectively combat skin damage when used in the preparation of drugs, health products, and cosmetics for treating skin damage.

[0018] Unless otherwise specified, the experimental methods used in the following examples are conventional methods; the materials and reagents used are commercially available unless otherwise specified.

[0019] Example 1. Validation of the anti-skin damage activity of chaetominine.

[0020] The structural formula of chamomile amine is: .

[0021] Wild-type AB zebrafish were used in a UVB experiment. The experimental groups were as follows: a control group (no radiation, no medication), a UV-damaged group (radiation, no medication), a VC group (radiation, medication), and a chaetominine group (radiation, medication). Each group contained at least 30 fish, and both the VC and chaetominine concentrations were 10 mM. After hatching (approximately 3 days post-hatching), the fish were exposed to 100 mJ / cm³ at 30-minute intervals. 2 UVB, 3 times.

[0022] Finally, the 4dpf zebrafish were anesthetized with 0.03% tricaine and transferred to a glass slide. After adjusting the zebrafish's body position, the damage to the caudal fins of the zebrafish was observed under a fluorescence microscope in bright field (the fins were compared with the fins of healthy non-experimental fish and subjectively classified as normal fins (at least 90% of the fins are intact), reduced fins (20%-90% intact), or missing fins (<20% intact)). The degree of caudal fin damage was quantitatively analyzed using ImageJ software.

[0023] ROS Levels: 4-day-first-floor (dpf) zebrafish were stained in 10 μg / mL DCFH-DA fluorescent probe solution at 28°C for 1 h in the dark. After washing with fresh culture medium and anesthetizing with tricaine, the zebrafish were photographed under a fluorescence microscope. The fluorescence intensity was then quantitatively analyzed using ImageJ software. Note: Higher fluorescence intensity indicates higher ROS levels and more oxygen free radicals in the zebrafish.

[0024] The degree of repair is then calculated using a formula, as follows: .

[0025] The results showed that the repair rate in the positive control group treated with vitamin C was 19.45% compared to the naturally repaired damage group. The repair rate in the experimental drug group treated with the compound chaetominine was 51.41% compared to the naturally repaired damage group, significantly higher than the positive control group. These results indicate that chaetominine has an anti-UV-induced skin damage effect.

Claims

1. Use of a Chaetocidin in the preparation of a cosmetic product for resisting skin damage.

2. Use of a Chaetamulin according to claim 1 in the preparation of a cosmetic product against skin damage, characterized in that, The skin damage includes ultraviolet-induced skin damage and related complications.

3. Use of a Chaetamulin according to claim 1 in the preparation of a cosmetic product against skin damage, characterized in that, The cosmetic product is a skin repair type skin care product or cosmetic product.

4. Use of a Chaetocidin in the preparation of a pharmaceutical product for resisting skin damage.

5. Use of a Chaetominine according to claim 4 for the preparation of a medicament for the treatment of skin lesions, characterized in that, The skin damage includes ultraviolet-induced skin damage and related complications.

6. Use of a Chaetamulin according to claim 4 for the preparation of a medicament for the treatment of skin lesions, characterized in that, The pharmaceutical product type includes injection, drip infusion, powder injection, granules, tablets, instant powder, powder, oral liquid, sugar-coated tablets, film-coated tablets, enteric-coated tablets, buccal tablets, granules, pills, ointments, Dan agent, spray, dripping pills, disintegrating agent, oral disintegrating tablets and pellets.

7. Use of a Chaetocidin in the preparation of a health care product for resisting skin damage.

8. The use of a Chaetominum amine according to claim 7 in the preparation of a health product against skin damage, characterized by the fact that, The skin damage includes ultraviolet-induced skin damage and related complications.

9. The use of a Chaetominum amine according to claim 8 in the preparation of a health product against skin damage, characterized by the fact that, The health care product is a health care product for preventing skin damage.

10. The use of a Chaetominum amine according to claim 7 in the manufacture of a health care product for the prevention of skin damage, characterized in that, It comprises Chaetocidin and a carrier acceptable in the health care product.

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