Compound traditional Chinese medicine oral liquid for loosening bowel to relieve constipation and preparation process of compound traditional Chinese medicine oral liquid

Abstract

The invention relates to the technical field of traditional Chinese medicine oral liquid preparation, in particular to compound traditional Chinese medicine oral liquid for relaxing bowel and a preparation process thereof. The preparation process of the compound traditional Chinese medicine oral liquid for relaxing bowel comprises the following steps: preparing the achyranthes aspera extract and the radix glehniae stem and leaf extract; preparing glutinous rice banana fermentation liquor; preparing fermented litchi juice; preparing the compound traditional Chinese medicine oral liquid. The preparation method comprises the following steps: pulping glutinous rice banana pulp, carrying out enzymolysis by using enzyme, adding a complex microbial inoculant, fermenting to prepare a glutinous rice banana fermentation broth, mixing the glutinous rice banana fermentation broth with a tuber fleeceflower stem extract and the like to prepare the composite traditional Chinese medicine oral liquid, and the anthraquinone component of tuber fleeceflower stem has a strong effect, is biased to'treatment of symptoms', and rapidly solves symptoms. And the glutinous rice banana fermentation liquor focuses on treating the root and recovering the health function of the intestinal tract for a long time by regulating flora and nourishing the intestinal tract, and the combination of the glutinous rice banana fermentation liquor and the glutinous rice banana fermentation liquor can realize the unification of quick effect taking and long-term regulation to achieve the synergistic bowel relaxing effect.

Description

Technical Field

[0001] This invention relates to the field of traditional Chinese medicine oral liquid preparation technology, specifically to a compound traditional Chinese medicine oral liquid for relieving constipation and its preparation process. Background Technology

[0002] Constipation is a common digestive system disorder in clinical practice, especially functional constipation. Its incidence rate remains high among middle-aged and elderly people, sedentary people, and people with unbalanced diets. Long-term constipation can easily lead to abdominal distension, intestinal flora imbalance, and even increase the risk of intestinal diseases, significantly impacting the quality of life of patients.

[0003] Currently, laxative products are mainly divided into two categories: traditional Chinese medicine preparations and chemical drugs. Among them, chemical laxatives (such as polyethylene glycol and bisacodyl) are fast-acting, but they mostly achieve bowel movements by stimulating the intestinal mucosa or increasing intestinal osmotic pressure, lacking the effect of repairing intestinal function. Moreover, long-term use can easily disrupt the intestinal microecological balance, causing side effects such as abdominal pain and diarrhea, thus limiting the applicable population. On the other hand, traditional Chinese medicine preparations are mostly based on single laxative herbs (such as hemp seed and apricot kernel) or simple combinations (such as hemp seed pills and laxative pills). Although they are relatively mild, they generally have the problems of slow onset of action and single target of action. In addition, long-term use may lead to intestinal function dependence and electrolyte imbalance, and cannot achieve the fundamental pathogenesis of constipation such as "spleen deficiency and qi stagnation".

[0004] Therefore, there is a need to propose a compound traditional Chinese medicine oral liquid for relieving constipation that is fast-acting and has few side effects, as well as its preparation process. Summary of the Invention

[0005] In view of the shortcomings of the existing technology, the purpose of this invention is to provide a compound traditional Chinese medicine oral liquid for relieving constipation and its preparation process.

[0006] This invention provides a preparation process for a compound traditional Chinese medicine oral liquid for relieving constipation, comprising the following steps:

[0007] S1: Preparation of Achyranthes bidentata extract and Adenophora stricta stem and leaf extract

[0008] The stems and leaves of Achyranthes bidentata and Adenophora stricta were extracted with ethanol solution to obtain Achyranthes bidentata extract and Adenophora stricta stem and leaf extract, respectively.

[0009] S2: Preparation of glutinous rice banana fermentation liquid

[0010] S2.1: After peeling the fresh glutinous banana fruit, use a pulping machine to crush and pulp the pulp, then add saccharifying enzyme, cellulase, pectinase and lipase, heat at 38-40℃ for 1-2 hours for enzymatic hydrolysis, then filter and pasteurize to obtain the enzymatic hydrolysate.

[0011] S2.2: Add white sugar to the above enzymatic hydrolysate, stir and mix evenly, then add compound bacterial agent, the above Achyranthes bidentata extract and North American ginseng stem and leaf extract, stir and mix evenly, and ferment at 20-25℃ for 18-20 days, stirring once every 3 days during the period. After fermentation is completed, filter and take the supernatant to obtain glutinous rice banana fermentation liquid.

[0012] S3: Preparation of fermented lychee juice

[0013] After the lychee pulp is made into lychee juice, it is inoculated with Lactobacillus casei for fermentation to obtain fermented lychee juice.

[0014] S4: Preparation of compound traditional Chinese medicine oral liquid

[0015] First, extract Polygonum multiflorum vine with ethanol solution to prepare Polygonum multiflorum vine extract. Then, add Polygonum multiflorum vine extract and the above fermented lychee juice to the above fermented banana syrup. Then, add purified water, ginseng peptide, honey, inulin, citric acid and potassium sorbate, stir and mix evenly to obtain compound Chinese medicine oral liquid.

[0016] Furthermore, S1 specifically includes the following steps:

[0017] S1.1: Add 8-10 times the amount of water to Achyranthes bidentata and decoct for 80-90 minutes, then filter. Repeat 3 times, combine the filtrates to obtain Achyranthes bidentata water extract and water extract residue.

[0018] S1.2: Add 13-15 times the amount of 60% ethanol solution to the water residue, heat and reflux at 75-85℃ for 30-40 min, filter, repeat 3 times, combine the filtrates to obtain the ethanol extract of Achyranthes bidentata.

[0019] S1.3: The above-mentioned Achyranthes bidentata aqueous extract and Achyranthes bidentata alcoholic extract were stirred and mixed, concentrated under reduced pressure and dried to obtain Achyranthes bidentata extract;

[0020] S1.4: After washing, drying and crushing the stems and leaves of Glehnia littoralis, add them to a 70% ethanol solution at a material-to-liquid ratio of 1g:(25-35)mL. Extract by ultrasonic reflux at 65-75℃ for 40-50min. After filtration, concentrate and dry under reduced pressure to obtain the extract of Glehnia littoralis stems and leaves.

[0021] Furthermore, S3 specifically includes the following steps:

[0022] S3.1: Fresh lychee pulp is pulped and filtered, and sodium bicarbonate is added to the filtrate to adjust the pH to 6-6.5. After pasteurization, lychee juice is obtained.

[0023] S3.2: Press 10 6 Lactobacillus casei was inoculated into the above lychee juice at CFU / mL and fermented at 30-32℃ for 22-24 hours to obtain fermented lychee juice.

[0024] Furthermore, S4 specifically includes the following steps:

[0025] S4.1: After washing, drying and crushing Polygonum multiflorum, add it to 75% ethanol solution at a material-to-liquid ratio of 1g:(15-20)mL, heat and reflux at 70-80℃ for 1-2h, repeat 2-3 times, combine the filtrates, recover the ethanol by vacuum distillation, and dry to obtain Polygonum multiflorum extract.

[0026] S4.2: Add the above-mentioned extract of Polygonum multiflorum and the fermented lychee juice obtained in step S3.2 to the fermented glutinous rice banana liquid, then add purified water, ginseng peptide, honey, inulin, citric acid and potassium sorbate, stir and mix evenly to obtain a compound Chinese medicine oral liquid.

[0027] Furthermore, the amount of saccharifying enzyme added is 0.1% of the weight of the banana pulp.

[0028] Furthermore, the amount of cellulase and pectinase added is equal to the amount of saccharifying enzyme added, and the amount of lipase added is 0.01-0.02% of the weight of the banana pulp.

[0029] Furthermore, the mass ratio of the enzymatic hydrolysate to white sugar is (2.2-2.4):1.

[0030] Furthermore, the compound microbial agent is composed of yeast, acetic acid bacteria and lactic acid bacteria in a mass ratio of (2-3):(1-3):1.

[0031] Furthermore, by mass percentage, the raw material composition of the compound traditional Chinese medicine oral liquid is as follows: 10-20% fermented glutinous rice banana liquid, 8-10% fermented lychee juice, 3-5% extract of Polygonum multiflorum, 2-3% ginseng peptide, 1-3% honey, 1-3% inulin, 0.2-0.4% citric acid, 0.1-0.2% potassium sorbate, and the balance being purified water.

[0032] A compound traditional Chinese medicine oral liquid for relieving constipation, which is prepared by the preparation process of a compound traditional Chinese medicine oral liquid for relieving constipation described in any one of the above-mentioned methods.

[0033] The present invention has the following advantages:

[0034] 1. In this invention, the pulp of the banana fruit is first pulped, then enzymatically hydrolyzed, and then fermented with a compound bacterial agent to prepare a banana fermentation liquid. This liquid is then mixed with extracts of Polygonum multiflorum (Hemiberleaf jasminoides) to prepare a compound traditional Chinese medicine oral liquid. Because the banana fermentation liquid is rich in oligosaccharides, dietary fiber, and various organic acids produced during fermentation, it can selectively promote the growth and proliferation of beneficial bacteria such as lactobacilli and bifidobacteria in the intestines, lower the intestinal pH, inhibit putrefactive bacteria, and promote the production of short-chain fatty acids, helping to repair the intestinal mucosa and maintain a healthy intestinal barrier. Meanwhile, the anthraquinone components in the Polygonum multiflorum extract are metabolized by the intestinal flora. After the process, its aglycones can directly stimulate the enteric nervous system of the colon wall, enhance intestinal peristalsis, and inhibit the absorption of sodium ions and water in the intestine, causing more water to remain in the intestinal lumen, increasing the water content of the feces, softening it, and further reducing the resistance to excretion. When the two are used together, the anthraquinone components of Polygonum multiflorum have a stronger effect and tend to "treat the symptoms" quickly, while the fermented liquid of glutinous rice banana focuses more on "treating the root cause" by regulating the flora and nourishing the intestines, restoring the healthy function of the intestines in the long term. Thus, it can achieve a unity of rapid onset and long-term conditioning, achieving a synergistic effect of lubricating the intestines and relieving constipation. Moreover, it does not contain chemical drugs and is less likely to produce side effects.

[0035] 2. In this invention, when Achyranthes bidentata extract and Glehnia littoralis stem and leaf extract are added to the enzymatic hydrolysate of banana glutinous rice, and then a compound microbial agent is added for fermentation, the saponins in the Achyranthes bidentata extract can increase the permeability of the cell membrane, helping nutrients to enter the bacterial cells more efficiently. Flavonoids can enhance the enzyme activity of fermenting bacteria and accelerate the conversion of carbohydrates in the banana glutinous rice hydrolysate. Meanwhile, the flavonoids and phenolic acids in the Glehnia littoralis stem and leaf extract can remove reactive oxygen species in the fermentation liquid, protect the enzymes produced by the fermenting bacteria from oxidation, maintain the continuous metabolic capacity of the bacterial community, and reduce the toxic damage of alcohol to the yeast itself, thus prolonging its logarithmic growth phase. When the two are combined, the Achyranthes bidentata extract "feeds the bacterial community and accelerates metabolism," while the Glehnia littoralis extract "protects the bacterial community and stabilizes the environment," so as to synergistically promote the fermentation activity of yeast, acetic acid bacteria, and lactic acid bacteria, which helps to improve the fermentation efficiency of banana glutinous rice hydrolysate, thereby increasing the yield of organic acids in the banana glutinous rice fermentation liquid.

[0036] 3. In this invention, fermented lychee juice is added to the fermented glutinous rice banana liquid and then mixed with other raw materials to make a compound traditional Chinese medicine oral liquid. On the one hand, the Lactobacillus casei in the fermented lychee juice can form a synergistic effect with the prebiotics in the fermented glutinous rice banana liquid, which helps exogenous probiotics to colonize and reproduce in the intestine. At the same time, the fermented lychee juice contains a large amount of organic acids such as lactic acid and acetic acid, which can lower the pH of the intestine, create an acidic environment that is not conducive to the growth of putrefactive bacteria and is conducive to the proliferation of beneficial bacteria, and gently stimulate the intestinal wall to promote peristalsis. On the other hand, after fermentation, the fructose, vitamins, minerals and other substances in lychee can be converted into smaller molecules that are more easily absorbed, providing energy and nutrition to the intestinal epithelial cells, helping to repair the damaged intestinal barrier, and improving constipation caused by poor intestinal function. This complements the function of the glutinous rice banana enzyme liquid in nourishing the intestine, and together they strengthen the foundation of intestinal health, thereby further improving the laxative effect of the compound traditional Chinese medicine oral liquid. Attached Figure Description

[0037] Figure 1 This is a flowchart illustrating the preparation process of the compound traditional Chinese medicine oral liquid for relieving constipation used in an embodiment of the present invention. Detailed Implementation

[0038] To enable those skilled in the art to better understand the technical solutions of this invention, the technical solutions of this invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of this invention.

[0039] Example 1: A preparation process for a compound traditional Chinese medicine oral liquid for relieving constipation, such as... Figure 1 As shown, it includes the following steps:

[0040] S1: Preparation of Achyranthes bidentata extract and Adenophora stricta stem and leaf extract

[0041] S1.1: Boil Achyranthes bidentata in 8 times the amount of water for 80 minutes, then filter, repeat 3 times, combine the filtrates to obtain Achyranthes bidentata water extract and water extract residue;

[0042] S1.2: Add 13 times the amount of 60% ethanol solution to the water residue, heat and reflux at 75℃ for 30 min, filter, repeat 3 times, combine the filtrates to obtain the ethanol extract of Achyranthes bidentata.

[0043] S1.3: The above-mentioned Achyranthes bidentata aqueous extract and Achyranthes bidentata alcoholic extract were stirred and mixed, concentrated under reduced pressure and dried to obtain Achyranthes bidentata extract;

[0044] S1.4: After washing, drying and crushing the stems and leaves of North American ginseng, add them to 70% ethanol solution at a material-to-liquid ratio of 1g:30mL. Extract by ultrasonic reflux at 70℃ for 45min. After filtration, concentrate and dry under reduced pressure to obtain North American ginseng stem and leaf extract.

[0045] S2: Preparation of glutinous rice banana fermentation liquid

[0046] S2.1: After peeling the fresh banana fruit, the pulp is crushed and pulped using a pulping machine. Then, saccharifying enzyme, cellulase, pectinase and lipase are added. The mixture is heated at 38°C for 1 hour for enzymatic hydrolysis. After filtration and pasteurization, the enzymatic hydrolysate is obtained. The amount of saccharifying enzyme added is 0.1% of the weight of the banana pulp, the amount of cellulase and pectinase added is equal to the amount of saccharifying enzyme added, and the amount of lipase added is 0.01% of the weight of the banana pulp.

[0047] S2.2: Add white sugar to the above enzymatic hydrolysate, stir and mix evenly, then add compound microbial agent, the above Achyranthes bidentata extract and North American ginseng stem and leaf extract, stir and mix evenly, and ferment at 20℃ for 18 days, stirring once every 3 days during the period. After fermentation is completed, filter and take the supernatant to obtain glutinous rice banana fermentation liquid, wherein the mass ratio of enzymatic hydrolysate to white sugar is 2.2:1, and the compound microbial agent is composed of yeast, acetic acid bacteria and lactic acid bacteria in a mass ratio of 2:1:1;

[0048] S3: Preparation of fermented lychee juice

[0049] S3.1: Fresh lychee pulp is pulped and filtered, and sodium bicarbonate is added to the filtrate to adjust the pH to 6. After pasteurization, lychee juice is obtained.

[0050] S3.2: Press 10 6 CFU / mL of Lactobacillus casei was added to the above lychee juice and fermented at 30°C for 22 hours to obtain fermented lychee juice.

[0051] S4: Preparation of compound traditional Chinese medicine oral liquid

[0052] S4.1: After washing, drying and crushing Polygonum multiflorum, add it to 75% ethanol solution at a material-to-liquid ratio of 1g:15mL, heat and reflux at 70℃ for 1h, repeat twice, combine the filtrates, recover the ethanol by vacuum distillation, and dry to obtain Polygonum multiflorum extract.

[0053] S4.2: Add the above-mentioned Polygonum multiflorum extract and the fermented lychee juice obtained in step S3.2 to the fermented glutinous rice banana liquid, then add purified water, ginseng peptide, honey, inulin, citric acid and potassium sorbate, stir and mix evenly to obtain a compound traditional Chinese medicine oral liquid. The raw material composition of the compound traditional Chinese medicine oral liquid by mass percentage is: 10% glutinous rice banana fermented liquid, 8% fermented lychee juice, 3% Polygonum multiflorum extract, 2% ginseng peptide, 1% honey, 1% inulin, 0.2% citric acid, 0.1% potassium sorbate, and the remainder is purified water.

[0054] Example 2: A preparation process for a compound traditional Chinese medicine oral liquid for relieving constipation, such as... Figure 1 As shown, it includes the following steps:

[0055] S1: Preparation of Achyranthes bidentata extract and Adenophora stricta stem and leaf extract

[0056] S1.1: Boil Achyranthes bidentata in 9 times the amount of water for 85 minutes, then filter, repeat 3 times, combine the filtrates to obtain Achyranthes bidentata water extract and water extract residue;

[0057] S1.2: Add 14 times the amount of 60% ethanol solution to the water residue, heat and reflux at 80℃ for 35 min, filter, repeat 3 times, combine the filtrates to obtain the ethanol extract of Achyranthes bidentata.

[0058] S1.3: The above-mentioned Achyranthes bidentata aqueous extract and Achyranthes bidentata alcoholic extract were stirred and mixed, concentrated under reduced pressure and dried to obtain Achyranthes bidentata extract;

[0059] S1.4: After washing, drying and crushing the stems and leaves of North American ginseng, add them to 70% ethanol solution at a material-to-liquid ratio of 1g:30mL. Extract by ultrasonic reflux at 70℃ for 45min. After filtration, concentrate and dry under reduced pressure to obtain North American ginseng stem and leaf extract.

[0060] S2: Preparation of glutinous rice banana fermentation liquid

[0061] S2.1: After peeling the fresh banana fruit, the pulp is crushed and pulped using a pulping machine. Then, saccharifying enzyme, cellulase, pectinase and lipase are added, and the mixture is heated at 39°C for 1.5 hours for enzymatic hydrolysis. After filtration and pasteurization, the enzymatic hydrolysate is obtained. The amount of saccharifying enzyme added is 0.1% of the weight of the banana pulp, the amount of cellulase and pectinase added is equal to the amount of saccharifying enzyme added, and the amount of lipase added is 0.015% of the weight of the banana pulp.

[0062] S2.2: Add white sugar to the above enzymatic hydrolysate, stir and mix evenly, then add the compound microbial agent, the above Achyranthes bidentata extract and North American ginseng stem and leaf extract, stir and mix evenly, and ferment at 23℃ for 19 days, stirring once every 3 days during the period. After fermentation is completed, filter and take the supernatant to obtain the glutinous rice banana fermentation liquid, wherein the mass ratio of enzymatic hydrolysate to white sugar is 2.3:1, and the compound microbial agent is composed of yeast, acetic acid bacteria and lactic acid bacteria in a mass ratio of 2.5:2:1;

[0063] S3: Preparation of fermented lychee juice

[0064] S3.1: Fresh lychee pulp is pulped and filtered, and sodium bicarbonate is added to the filtrate to adjust the pH to 6.3. After pasteurization, lychee juice is obtained.

[0065] S3.2: Press 10 6 CFU / mL of Lactobacillus casei was added to the above lychee juice and fermented at 31°C for 23 hours to obtain fermented lychee juice.

[0066] S4: Preparation of compound traditional Chinese medicine oral liquid

[0067] S4.1: After washing, drying and crushing Polygonum multiflorum, add it to 75% ethanol solution at a material-to-liquid ratio of 1g:17.5mL, heat and reflux at 75℃ for 1.5h, repeat twice, combine the filtrates, recover the ethanol by vacuum distillation, and dry to obtain Polygonum multiflorum extract.

[0068] S4.2: Add the above-mentioned Polygonum multiflorum extract and the fermented lychee juice obtained in step S3.2 to the fermented glutinous rice banana liquid, then add purified water, ginseng peptide, honey, inulin, citric acid and potassium sorbate, stir and mix evenly to obtain a compound traditional Chinese medicine oral liquid. The raw material composition of the compound traditional Chinese medicine oral liquid by mass percentage is: 15% glutinous rice banana fermented liquid, 9% fermented lychee juice, 4% Polygonum multiflorum extract, 2.5% ginseng peptide, 2% honey, 2% inulin, 0.3% citric acid, 0.15% potassium sorbate, and the remainder is purified water.

[0069] Example 3: A preparation process for a compound traditional Chinese medicine oral liquid for relieving constipation, such as... Figure 1 As shown, it includes the following steps:

[0070] S1: Preparation of Achyranthes bidentata extract and Adenophora stricta stem and leaf extract

[0071] S1.1: Boil Achyranthes bidentata in 10 times the amount of water for 90 minutes, then filter, repeat 3 times, combine the filtrates to obtain Achyranthes bidentata water extract and water extract residue;

[0072] S1.2: Add 15 times the amount of 60% ethanol solution to the water residue, heat and reflux at 85℃ for 40 min, filter, repeat 3 times, combine the filtrates to obtain the ethanol extract of Achyranthes bidentata.

[0073] S1.3: The above-mentioned Achyranthes bidentata aqueous extract and Achyranthes bidentata alcoholic extract were stirred and mixed, concentrated under reduced pressure and dried to obtain Achyranthes bidentata extract;

[0074] S1.4: After washing, drying and crushing the stems and leaves of North American ginseng, add them to 70% ethanol solution at a material-to-liquid ratio of 1g:35mL. Extract by ultrasonic reflux at 75℃ for 50min. After filtration, concentrate and dry under reduced pressure to obtain North American ginseng stem and leaf extract.

[0075] S2: Preparation of glutinous rice banana fermentation liquid

[0076] S2.1: After peeling the fresh banana fruit, the pulp is crushed and pulped using a pulping machine. Then, saccharifying enzyme, cellulase, pectinase and lipase are added, and the mixture is heated at 40℃ for 2 hours for enzymatic hydrolysis. After filtration and pasteurization, the enzymatic hydrolysate is obtained. The amount of saccharifying enzyme added is 0.1% of the weight of the banana pulp, the amount of cellulase and pectinase added is equal to the amount of saccharifying enzyme added, and the amount of lipase added is 0.02% of the weight of the banana pulp.

[0077] S2.2: Add white sugar to the above enzymatic hydrolysate, stir and mix evenly, then add compound microbial agent, the above Achyranthes bidentata extract and North American ginseng stem and leaf extract, stir and mix evenly, and ferment at 25℃ for 20 days, stirring once every 3 days during the period. After fermentation is completed, filter and take the supernatant to obtain glutinous rice banana fermentation liquid, wherein the mass ratio of enzymatic hydrolysate to white sugar is 2.4:1, and the compound microbial agent is composed of yeast, acetic acid bacteria and lactic acid bacteria in a mass ratio of 3:3:1;

[0078] S3: Preparation of fermented lychee juice

[0079] S3.1: Fresh lychee pulp is pulped and filtered, and sodium bicarbonate is added to the filtrate to adjust the pH to 6.5. After pasteurization, lychee juice is obtained.

[0080] S3.2: Press 10 6 CFU / mL of Lactobacillus casei was added to the above lychee juice and fermented at 32°C for 24 hours to obtain fermented lychee juice.

[0081] S4: Preparation of compound traditional Chinese medicine oral liquid

[0082] S4.1: After washing, drying and crushing Polygonum multiflorum, add it to 75% ethanol solution at a material-to-liquid ratio of 1g:20mL, heat and reflux at 80℃ for 2h, repeat 3 times, combine the filtrates, recover the ethanol by vacuum distillation, and dry to obtain Polygonum multiflorum extract;

[0083] S4.2: Add the above-mentioned Polygonum multiflorum extract and the fermented lychee juice obtained in step S3.2 to the fermented glutinous rice banana liquid, then add purified water, ginseng peptide, honey, inulin, citric acid and potassium sorbate, stir and mix evenly to obtain a compound traditional Chinese medicine oral liquid. The raw material composition of the compound traditional Chinese medicine oral liquid by mass percentage is: 20% glutinous rice banana fermented liquid, 10% fermented lychee juice, 5% Polygonum multiflorum extract, 3% ginseng peptide, 3% honey, 3% inulin, 0.4% citric acid, 0.2% potassium sorbate, and the remainder is purified water.

[0084] Comparative Example 1 differs from Example 1 in that the fermented glutinous rice banana liquid in step S4.2 is replaced with an equal amount of Polygonum multiflorum extract.

[0085] Comparative Example 2 differs from Example 1 in that the extract of Polygonum multiflorum in step S4.2 is replaced with an equal amount of Banana glutinosa fermentation liquid.

[0086] Comparative Example 3 differs from Example 1 in that the extract of North American ginseng stems and leaves in step S2.2 is replaced with an equal amount of Achyranthes bidentata extract.

[0087] Comparative Example 4 differs from Example 1 in that the Achyranthes bidentata extract in step S2.2 is replaced with an equal amount of Glehnia littoralis stem and leaf extract.

[0088] Comparative Example 5 differs from Example 1 in that the fermented lychee juice in step S4.2 is removed.

[0089] Comparative Example 6 differs from Example 1 in that the fermented lychee juice in step S4.2 is replaced with an equal amount of lychee juice.

[0090] Test example:

[0091] Test 1: Animal defecation experiment

[0092] SPF-grade male ICR mice were housed in an environment with a temperature of 22°C and a humidity of 60%, with alternating light and dark conditions every 12 hours, and were given an acclimatization diet for one week, during which they had free access to water and food. After the adaptive feeding period, the mice were randomly divided into 9 groups of 10 mice each: blank control group, constipation model group, Example 1 group, Example 2 group, Example 3 group, Comparative Example 1 group, Comparative Example 2 group, Comparative Example 5 group, and Comparative Example 6 group. The blank control group and constipation model group were administered distilled water by gavage at 10 mL / kg bw. The Example 1-3 group, Comparative Example 1-2 group, and Comparative Example 5-6 group were administered the compound Chinese medicine oral liquid prepared in Example 1-3, Comparative Example 1-2, and Comparative Example 5-6 by gavage at 10 mL / kg bw. After 7 days of gavage, on the 7th day, the mice in each group were fasted for 16 hours and given free access to water. Then, except for the blank control group which was administered distilled water by gavage at 10 mg / kg bw, the other groups were administered loperamide hydrochloride by gavage at 10 mg / kg bw to establish a mouse constipation model. After 30 minutes, except for the blank group and the constipation model group, which were administered 5% ink prepared with distilled water at 10 mL / kg·bw, the other groups were administered 5% ink prepared with compound traditional Chinese medicine oral liquid at 10 mL / kg·bw (i.e., 5% ink prepared with compound traditional Chinese medicine oral liquid prepared in Example 1, 5% ink prepared with compound traditional Chinese medicine oral liquid prepared in Example 2, 5% ink prepared with compound traditional Chinese medicine oral liquid prepared in Example 3, 5% ink prepared with compound traditional Chinese medicine oral liquid prepared in Example 3, 5% ink prepared with compound traditional Chinese medicine oral liquid prepared in Comparative Example 1, 5% ink prepared with compound traditional Chinese medicine oral liquid prepared in Comparative Example 2, 5% ink prepared with compound traditional Chinese medicine oral liquid prepared in Comparative Example 5, and 5% ink prepared with compound traditional Chinese medicine oral liquid prepared in Comparative Example 6). After administration, mice were kept on a fasting diet and allowed free access to water. Over the next 6 hours, the time of the first excretion of black stool, the total weight of wet black stool, and the total number of black stool particles were observed and recorded for each group. The average values ​​were taken. After the total wet black stool of each group was dried to 60%, the weight of the dried black stool was measured, and the water content of the black stool was calculated using the following formula:

[0093] Moisture content of black stool (%) = (Wet black stool mass - Dry black stool mass) / Wet black stool mass × 100%.

[0094] Table 1: Results of the defecation experiment in mice

[0095] Time to first black stool discharge (min) Moisture content of black stool (%) Stool particle count (particles) Example 1 122.6 25.7 20.2 Example 2 119.3 26.3 20.7 Example 3 117.5 26.8 21.4 Comparative Example 1 213.4 16.3 14.2 Comparative Example 2 188.7 23.2 15.8 Comparative Example 5 186.9 24.3 16.7 Comparative Example 6 185.3 24.1 17.1 Blank group 105.2 25.8 19.2 Constipation model group 263.5 14.6 8.7

[0096] As shown in Table 1, when only one of Polygonum multiflorum extract or Banana glutinosa fermentation liquid was added in Comparative Examples 1 and 2, the time for mice to first excrete black stool was longer than that in Example 1, and the water content and number of black stool particles were lower than those in Example 1. This shows that when Banana glutinosa fermentation liquid and Polygonum multiflorum extract are used together, they can achieve a synergistic effect of lubricating the intestines and relieving constipation.

[0097] In Comparative Example 5, without the addition of fermented lychee juice, the time it took for mice to first excrete black stool was longer than in Example 1, while the water content and number of black stool particles were lower than in Example 1. In Comparative Example 6, after replacing fermented lychee juice with lychee juice, the time it took for mice to first excrete black stool, the water content of black stool, and the number of black stool particles were comparable to those in Comparative Example 5. This shows that only by adding fermented lychee juice to the fermented glutinous rice banana liquid and then mixing it with other raw materials to make a compound traditional Chinese medicine oral liquid can the laxative effect of the compound traditional Chinese medicine oral liquid be further improved. Unfermented lychee juice does not have this effect.

[0098] Test 2: The total organic acid content (including oxalic acid, tartaric acid, formic acid, lactic acid, acetic acid, maleic acid and succinic acid) in the fermentation liquid of banana prepared in Examples 1-3 and Comparative Examples 3-4 was determined. The test was repeated three times and the average value was taken. The results are shown in Table 2.

[0099] Table 2: Results of Total Organic Acid Content in Banana Fermentation Broth

[0100] Total organic acid content (g / L) Example 1 146.4 Example 2 147.7 Example 3 148.3 Comparative Example 3 121.6 Comparative Example 4 118.9

[0101] As shown in Table 2 above, when only one of the extracts of Achyranthes bidentata or the stem and leaf extract of Adenophora stricta was added to the enzymatic hydrolysate of banana glutinous rice in Comparative Examples 3 and 4 for fermentation, the total organic acid content in the fermented glutinous rice banana broth was significantly lower than that in Example 1. This indicates that when Achyranthes bidentata extract and Adenophora stricta stem and leaf extract are added to the enzymatic hydrolysate of banana glutinous rice, and then a compound microbial agent is added for fermentation, the Achyranthes bidentata extract and the Adenophora stricta extract can synergistically promote the fermentation activity of yeast, acetic acid bacteria and lactic acid bacteria, which helps to improve the fermentation efficiency of banana glutinous rice hydrolysate, thereby increasing the yield of organic acids in the fermented glutinous rice banana broth.

[0102] It should be understood that those skilled in the art can make improvements or modifications based on the above description, and all such improvements and modifications should fall within the protection scope of the appended claims. Parts not described in detail in this specification are prior art known to those skilled in the art.

Claims

1. A preparation process of a compound traditional Chinese medicine oral liquid for lubricating intestines and defecating, characterized in that, Comprising the following steps: S1: preparing radix adiantifolii and radix ophiopogonis stem and leaf extract Radix adiantifolii and radix ophiopogonis stem and leaf are extracted with ethanol solution respectively to obtain radix adiantifolii extract and radix ophiopogonis stem and leaf extract; S2: preparing fermenting liquid of canarius incanus S2.1: after peeling the fresh fruit of canarius incanus fruit, the pulp is broken and pulped using a pulper, then saccharifying enzyme, cellulase, pectinase and lipase are added, heated for enzymolysis at 38-40℃ for 1-2h, then filtered and pasteurized to obtain the enzymolysis liquid; S2.2: add white granulated sugar to the above-mentioned enzymolysis liquid, stir and mix evenly, then add composite microbial inoculum, the above-mentioned radix adiantifolii extract and radix ophiopogonis stem and leaf extract, stir and mix evenly, and ferment at 20-25℃ for 18-20 days, stir every 3 days during the fermentation, after the fermentation is completed, filter and take the supernatant to obtain the fermenting liquid of canarius incanus; S3: preparing fermented litchi juice After the litchi pulp is made into litchi juice, it is inoculated with lactobacillus casei to ferment and obtain fermented litchi juice; S4: preparing composite traditional Chinese medicine oral liquid First, extract morindae officinalis with ethanol solution to prepare morindae officinalis extract, then add it and the above-mentioned fermented litchi juice into the above-mentioned fermenting liquid of canarius incanus, then add purified water, ginseng peptide, honey, chrysanthemum powder, citric acid and potassium sorbate, stir and mix evenly to obtain the composite traditional Chinese medicine oral liquid.

2. The preparation process of the compound traditional Chinese medicine oral liquid for lubricating intestines and defecation according to claim 1, characterized in that, S1 specifically comprises the following steps: S1.1: decoct radix adiantifolii with 8-10 times the amount of water for 80-90 min, then filter, repeat 3 times, combine the filtrates to obtain radix adiantifolii water extract and water residue; S1.2: add 13-15 times the amount of 60% ethanol solution to the water residue, heat and reflux extract at 75-85℃ for 30-40 min, and filter, repeat 3 times, combine the filtrates to obtain radix adiantifolii alcohol extract; S1.3: stir and mix the above-mentioned radix adiantifolii water extract and radix adiantifolii alcohol extract, concentrate under reduced pressure and dry to obtain radix adiantifolii extract; S1.4: after washing, drying and crushing radix ophiopogonis stem and leaf, add 70% ethanol solution according to the solid-liquid ratio of 1g:(25-35)mL, heat and ultrasonic-assisted reflux extract at 65-75℃ for 40-50 min, filter, concentrate under reduced pressure and dry to obtain radix ophiopogonis stem and leaf extract.

3. The preparation process of the compound traditional Chinese medicine oral liquid for lubricating intestines and defecation according to claim 2, characterized in that, S3 specifically comprises the following steps: S3.1: break and filter fresh litchi pulp, then add sodium bicarbonate to the filtrate to adjust the pH to 6-6.5, pasteurize to obtain litchi juice; S3.2: 10 6 CFU / mL into the above litchi juice, and fermented at 30-32°C for 22-24h to obtain the fermented litchi juice.

4. The preparation process of the compound traditional Chinese medicine oral liquid for lubricating intestines and defecation according to claim 1, characterized in that, S4 specifically comprises the following steps: S4.1: after washing, drying and crushing morindae officinalis, add 75% ethanol solution according to the solid-liquid ratio of 1g:(15-20)mL, heat and reflux extract at 70-80℃ for 1-2h, repeat 2-3 times, combine the filtrates, recover ethanol by distillation under reduced pressure, and dry to obtain morindae officinalis extract; S4.2: add the above-mentioned morindae officinalis extract and fermented litchi juice prepared in step S3.2 into the fermenting liquid of canarius incanus, then add purified water, ginseng peptide, honey, chrysanthemum powder, citric acid and potassium sorbate, stir and mix evenly to obtain the composite traditional Chinese medicine oral liquid.

5. The preparation process of the compound traditional Chinese medicine oral liquid for lubricating intestines and defecation according to claim 1, characterized in that, The amount of saccharifying enzyme added is 0.1% of the mass of canarius incanus pulp.

6. The preparation process of the compound traditional Chinese medicine oral liquid for lubricating intestines and defecation according to claim 1, characterized in that, The cellulase and pectinase are added in an amount equal to the amount of saccharifying enzyme, and the lipase is added in an amount of 0.01-0.02% of the mass of the banana pulp.

7. The preparation process of the compound traditional Chinese medicine oral liquid for lubricating intestines and defecation according to claim 1, characterized in that, The mass ratio of the enzymatic hydrolysate to white granulated sugar is (2.2-2.4):

1.

8. The preparation process of the compound traditional Chinese medicine oral liquid for lubricating intestines and defecation according to claim 1, characterized in that, The complex microbial agent is composed of yeast, acetic acid bacillus and lactic acid bacteria in a mass ratio of (2-3):(1-3):

1.

9. The preparation process of the compound traditional Chinese medicine oral liquid for lubricating intestines and defecation according to claim 4, characterized in that, The raw materials of the complex traditional Chinese medicine oral liquid are composed of, in mass percentage, 10-20% of the banana fermenting liquid, 8-10% of the fermented litchi juice, 3-5% of the extract of Marsdenia tenacissima, 2-3% of ginseng peptide, 1-3% of honey, 1-3% of dandelion powder, 0.2-0.4% of citric acid, 0.1-0.2% of potassium sorbate, and the balance of purified water.

10. A compound traditional Chinese medicine oral liquid for lubricating intestines and defecating, characterized in that, The complex traditional Chinese medicine oral liquid for lubricating intestines and defecating is prepared by the preparation process of any one of claims 1-9.

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