Selenium-rich pea sprouting vegetable culture solution and biological enhancement method
By combining nano-composite selenium particles with flavonoid photosensitizing enhancers, the problems of low selenium conversion efficiency and stability in pea sprouts have been solved, enabling efficient and safe selenium-enriched cultivation and ensuring the food safety of pea sprouts.
Patent Information
- Application Number
- CN202511857408.5
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-12-10
- Publication Date
- 2026-01-20
- Estimated Expiration
- Not applicable · inactive patent
AI Technical Summary
In existing technologies, the method of soaking or spraying pea sprouts with inorganic selenium salt solution has low selenium conversion efficiency, unstable selenium content, and is toxic to plant cells. It is also cumbersome to operate and poses food safety risks.
By using nano-composite selenium particles and flavonoid photosensitizing factors, and through physiological activation, contact with nano-selenium sources, photosensitivity promotion, and staged selenium control treatment, combined with stable regulation of the growth environment, selenium-enriched pea sprouts are formed.
It improves the conversion efficiency and stability of selenium, avoids the toxic effects of inorganic selenium on plant cells, realizes efficient, simple and safe selenium-enriched cultivation, and ensures the food safety of pea sprouts.
Smart Images
Figure CN121359635A_ABST
Abstract
Description
TECHNICAL FIELD
[0001] The application belongs to the field of agricultural biotechnology, and particularly relates to a culture solution for selenium-rich pea sprouts and a biological strengthening method. BACKGROUND
[0002] Selenium is essential to human health, and cultivating selenium-rich vegetables through agricultural biological strengthening technology is an effective means to supplement dietary selenium. Pea sprouts grow rapidly and have a tender taste, and are an ideal carrier for selenium nutrition strengthening.
[0003] Existing technologies mainly use inorganic selenium salt solutions to soak or spray sprouts. This method has low selenium conversion efficiency and unstable selenium content in sprouts. Inorganic selenium has high toxicity to plant cells and easily inhibits seed germination and seedling growth. The operation process is complicated and has food safety risks. Therefore, the application provides a culture solution for selenium-rich pea sprouts and a biological strengthening method to solve the above problems. SUMMARY
[0004] In view of the deficiencies of the prior art, the application provides a culture solution for selenium-rich pea sprouts and a biological strengthening method, which solves the technical problem of how to use nano-composite selenium particles and photosensitive enhancement factors to improve the selenium conversion efficiency and stability of selenium-rich pea sprouts.
[0005] To solve the above technical problems, the application is implemented through the following technical solutions.
[0006] The culture solution for selenium-rich pea sprouts comprises the following components in percentage by mass: Nano-composite selenium particles: 0.02%-0.08%.
[0007] Flavonoid photosensitive enhancement factor: 0.10%-0.25%.
[0008] Seaweed extract: 0.10%-0.30%.
[0009] Amino acid hydrolysate: 0.20%-0.50%.
[0010] Sodium citrate: 0.05%-0.20%.
[0011] Potassium dihydrogen phosphate: 0.05%-0.20%.
[0012] Deionized water: 98.5%-99.5%.
[0013] The application is further provided as follows: the carrier of the nano-composite selenium particles is sodium carboxymethyl cellulose, and the average particle size of the nano-composite selenium particles is 80nm-150nm.
[0014] The flavonoids photosensitive enhancer includes rutin and quercetin, and the mass ratio of the rutin and the quercetin is 3:4.
[0015] A biological strengthening method of selenium-rich pea sprouts comprises the following steps: S1. Physiological activation treatment is performed on pea seeds to form physiological activation seeds.
[0016] S2. Nano-composite selenium source contact treatment is performed on the physiological activation seeds to form nano-selenium loaded seeds, the nano-selenium loaded seeds have a controllable release deposition layer, and the nano-composite selenium source contact treatment adds nano-composite selenium particles.
[0017] S3. Photosensitive promotion treatment is performed on the nano-selenium loaded seeds to form photo-activated selenium conversion sprouts, and the photosensitive promotion treatment comprises adding flavonoids photosensitive enhancer.
[0018] S4. Stage control selenium treatment is performed on the photo-activated selenium conversion sprouts to form selenium absorption growth sprouts, and the stage control selenium treatment adopts a stage adjustment selenium supply mode.
[0019] S5. Growth environment stable regulation treatment is performed on the selenium absorption growth sprouts to form selenium-rich pea sprouts, and the growth environment stable regulation treatment comprises adjusting growth environment parameters.
[0020] The application further provides that the physiological activation treatment comprises the following steps: S11. The pea seeds are placed in warm water for preliminary activation to form first-stage treatment seeds, the temperature of the warm water is 45-55 DEG C, and the soaking time of the preliminary activation is 10-15 minutes.
[0021] S12. The first-stage treatment seeds are added into a gibberellin solution for soaking to form second-stage treatment seeds, the concentration of the gibberellin solution is 50-100 mg / L, the soaking temperature is 25-30 DEG C, and the soaking time is 3-5 hours.
[0022] S13. The second-stage treatment seeds are washed with sterile water, and surface water is filtered to form the physiological activation seeds, and the washing is performed 2-3 times.
[0023] The application further provides that the particle size of the nano-composite selenium particles is 80-150 nm, and the nano-composite selenium particles are attached to the seed coat surface of the physiological activation seeds to form the controllable release deposition layer.
[0024] The application is further provided with the photosensitive promotion treatment, which comprises loading the nano selenium into a photobioreactor for culture, adding the flavonoid photosensitive enhancer into the photobioreactor for light reaction to form the photoactivated selenium conversion sprout, the photoperiod of the light reaction is 16 hours of light and 8 hours of darkness, the light intensity of the light reaction is 4000 Lux-5000 Lux, the environmental humidity is 75%-80%, and the culture time is 3 days.
[0025] The application is further provided with the light reaction using an LED light source, the LED light source comprises red light and blue light, the wavelength of the red light is 660nm±10nm, the wavelength of the blue light is 450nm±10nm, and the light intensity ratio of the red light to the blue light is 7:3.
[0026] The application is further provided with the stage adjustment selenium supply mode, which comprises a first stage and a second stage, the applied selenium concentration of the first stage is 0.01mg / L-0.05mg / L, and the maintenance time is 24 hours-36 hours, the applied selenium concentration of the second stage is 0.05mg / L-0.20mg / L, and the maintenance time is 48 hours-72 hours.
[0027] The application is further provided with the growth environment stable regulation and control treatment, which comprises placing the selenium absorption and growth sprout in a periodic temperature environment with a diurnal temperature difference of 8℃-12℃ for culture for 5 days-7 days, the daytime temperature of the periodic temperature environment is 22℃±1℃, the light time is 14 hours-16 hours, the nighttime temperature is 12℃±1℃, the relative humidity of the periodic temperature environment is 65%-75%, and the carbon dioxide concentration is 800ppm-1000ppm.
[0028] The application has the following beneficial effects.
[0029] The application improves the selenium absorption and conversion efficiency of the pea sprout by using the nano composite selenium particles and the flavonoid photosensitive enhancer. The nano composite selenium particles can form a controllable release deposition layer on the seed coat surface, reduce the toxicity of selenium, and avoid the negative effects of traditional inorganic selenium on plant cells. The introduction of the photosensitive enhancer promotes the organic conversion of selenium, improves the selenium content and growth rate of the selenium-rich pea sprout.
[0030] Through the stage control selenium treatment and the stable regulation and control of the growth environment, the application ensures that the pea sprout stably absorbs and uniformly distributes selenium elements in the whole growth process, avoids the instability of the selenium content, accurately adjusts the selenium supply concentration, environmental temperature, humidity and other parameters during the culture process, improves the accumulation efficiency of selenium in the sprout, reduces the problems of complicated operation and food safety risks in the traditional method, and realizes an efficient, simple and safe selenium-rich planting mode. Attached Figure Description
[0031] To more clearly illustrate the technical solutions of the embodiments of the present invention, the accompanying drawings used in the description of the embodiments will be briefly introduced below.
[0032] Figure 1 This is a diagram showing the composition of a culture medium for selenium-enriched pea sprouts and a biofortification method.
[0033] Figure 2 This is a flowchart of a culture medium for selenium-enriched pea sprouts and a biofortification method.
[0034] Figure 3 This image shows the physiological activation treatment in the culture medium and biofortification method for a selenium-enriched pea sprout. Detailed Implementation
[0035] The technical solutions of the present invention will be described below with reference to the accompanying drawings. The described embodiments are only some embodiments of the present invention, and not all embodiments.
[0036] Example 1 Please see Figure 1 This invention relates to a culture medium for selenium-enriched pea sprouts, comprising, by weight percentage, the following components: Nanocomposite selenium particles: 0.02%.
[0037] Flavonoid photosensitizing factor: 0.10%.
[0038] Seaweed extract: 0.10%.
[0039] Amino acid hydrolysate: 0.20%.
[0040] Sodium citrate: 0.05%.
[0041] Potassium dihydrogen phosphate: 0.05%.
[0042] Deionized water: 99.48%.
[0043] The carrier of the nanocomposite selenium particles is sodium carboxymethyl cellulose, and the average particle size of the nanocomposite selenium particles is 80 nm. The flavonoid photosensitizing factors include rutin and quercetin, with a mass ratio of rutin to quercetin of 3:4.
[0044] A biofortification method for selenium-enriched pea sprouts includes: S1. Pea seeds undergo physiological activation treatment to form physiologically activated seeds. The steps of the physiological activation treatment are as follows: S11. The pea seeds are placed in warm water for initial activation to form first-stage treated seeds, the temperature of the warm water is 45℃, and the soaking time of the initial activation is 10 minutes.
[0045] S12. The first-stage treated seeds are soaked in a gibberellin solution to form second-stage treated seeds, the concentration of the gibberellin solution is 50 mg / L, the soaking temperature is 25℃, and the soaking time is 3 hours.
[0046] S13. The second-stage treated seeds are rinsed with sterile water and the surface moisture is filtered to form physiologically activated seeds, the rinsing is performed twice.
[0047] S2. The physiologically activated seeds are subjected to nano-composite selenium source contact treatment to form nano-selenium loaded seeds, the nano-selenium loaded seeds have a controllable release deposition layer, and nano-composite selenium particles are added in the nano-composite selenium source contact treatment. The particle size of the nano-composite selenium particles is 80 nm, and the nano-composite selenium particles adhere to the seed coat surface of the physiologically activated seeds to form a controllable release deposition layer.
[0048] S3. The nano-selenium loaded seeds are subjected to photosensitive promotion treatment to form light-activated selenium transformed sprouts, the photosensitive promotion treatment includes adding flavonoid photosensitive enhancer. The photosensitive promotion treatment includes placing the nano-selenium loaded seeds in a photobioreactor for culture, adding flavonoid photosensitive enhancer in the photobioreactor for light reaction to form light-activated selenium transformed sprouts, the light period of the light reaction is 16 hours of light and 8 hours of darkness, the light intensity of the light reaction is 4000 Lux, the environmental humidity is 75%, and the culture time is 3 days. The light reaction uses LED light source, the LED light source includes red light and blue light, the wavelength of the red light is 660 nm±10 nm, the wavelength of the blue light is 450 nm±10 nm, and the light intensity ratio of the red light to the blue light is 7:3.
[0049] S4. The light-activated selenium transformed sprouts are subjected to stage-controlled selenium treatment to form selenium-absorbed growth sprouts, the stage-controlled selenium treatment adopts a stage-adjusted selenium supply mode. The stage-adjusted selenium supply mode includes a first stage and a second stage, the applied selenium concentration of the first stage is 0.01 mg / L, and the maintenance time is 24 hours, the applied selenium concentration of the second stage is 0.05 mg / L, and the maintenance time is 48 hours.
[0050] S5. The selenium-absorbed growth sprouts are subjected to growth environment stable regulation and control treatment to form selenium-enriched pea sprout vegetables, the growth environment stable regulation and control treatment includes adjusting the growth environment parameters. The growth environment stable regulation and control treatment includes placing the selenium-absorbed growth sprouts in a periodic temperature environment with a diurnal temperature difference of 8℃ for culture for 5 days, the daytime temperature of the periodic temperature environment is 21℃, the light time is 14 hours, the nighttime temperature is 11℃, the relative humidity of the periodic temperature environment is 65%, and the carbon dioxide concentration is 800 ppm.
[0051] Result analysis and effect: Germination rate: under the treatment of low temperature, short time and low humidity, the germination rate of pea seeds in the experimental group was 96%, which was about 11% higher than the germination rate of about 85% in the traditional method.
[0052] Low humidity and low temperature conditions effectively reduce the evaporation loss of selenium, and avoid the toxic effects of high selenium content on seedlings.
[0053] Stability of selenium content: due to the treatment of low temperature and low humidity, the selenium content in the culture solution is stably controlled, the absorption of selenium is more uniform, and there is no excessive accumulation, which ensures the food safety of selenium-enriched pea sprouts.
[0054] Beneficial effects: High conversion rate and stable selenium content: the treatment of low temperature, short time and low humidity in the experimental group makes the conversion rate of selenium high and the selenium content stable, avoiding the problem of excessive evaporation or volatilization of selenium under high temperature and humidity.
[0055] Increase germination rate and growth efficiency: due to the low humidity condition in the experimental group, the growth of seedlings is more stable and not inhibited by excessive humidity, with a high germination rate.
[0056] Example 2 Please refer to Figure 1 On the basis of Example 1, a culture solution for selenium-enriched pea sprouts includes, by mass percentage, the following components: Nano-composite selenium particles: 0.08%.
[0057] Flavonoid photosensitive enhancer: 0.25%.
[0058] Seaweed extract: 0.30%.
[0059] Amino acid hydrolysate: 0.50%.
[0060] Sodium citrate: 0.20%.
[0061] Monopotassium phosphate: 0.20%.
[0062] Deionized water: 98.47%.
[0063] The carrier of nano-composite selenium particles is sodium carboxymethyl cellulose, and the average particle size of nano-composite selenium particles is 150 nm. Flavonoid photosensitive enhancer includes rutin and quercetin, and the mass ratio of rutin and quercetin is 3:4.
[0064] A method for biological strengthening of selenium-enriched pea sprouts includes: S1. Physiologically activating the pea seeds to form physiologically activated seeds. The steps of the physiologically activating treatment are as follows: S11. Placing the pea seeds in warm water for preliminary activation to form first-stage treated seeds, the temperature of the warm water being 55°C, and the soaking time of the preliminary activation being 15 minutes.
[0065] S12. Soaking the first-stage treated seeds in a gibberellin solution to form second-stage treated seeds, the concentration of the gibberellin solution being 100 mg / L, the soaking temperature being 30°C, and the soaking time being 5 hours.
[0066] S13. Rinsing the second-stage treated seeds with sterile water and filtering off the surface moisture to form the physiologically activated seeds, the number of rinsing times being 3.
[0067] S2. Contacting the physiologically activated seeds with a nano-composite selenium source to form nano-selenium loaded seeds, the nano-selenium loaded seeds having a controllable release deposition layer, and the nano-composite selenium source contacting treatment adding nano-composite selenium particles. The particle size of the nano-composite selenium particles is 150 nm, and the nano-composite selenium particles adhere to the seed coat surface of the physiologically activated seeds to form the controllable release deposition layer.
[0068] S3. Light sensitively promoting the nano-selenium loaded seeds to form light-activated selenium transformed sprouts, the light sensitively promoting treatment including adding a flavonoid light sensitization enhancer. The light sensitively promoting treatment includes placing the nano-selenium loaded seeds in a photobioreactor for culture, adding a flavonoid light sensitization enhancer in the photobioreactor for light reaction to form light-activated selenium transformed sprouts, the light reaction having a light period of 16 hours of light and 8 hours of darkness, a light intensity of 5000 Lux, an environmental humidity of 80%, and a culture time of 3 days. The light reaction uses an LED light source, the LED light source including red light and blue light, the red light having a wavelength of 660 nm±10 nm, the blue light having a wavelength of 450 nm±10 nm, and the light intensity ratio of the red light to the blue light being 7:3.
[0069] S4. Staging the light-activated selenium transformed sprouts to form selenium-absorbing growth sprouts, the staging the light-activated selenium transformed sprouts to form selenium-absorbing growth sprouts using a stage-adjusted selenium supply mode. The stage-adjusted selenium supply mode includes a first stage and a second stage, the first stage having an applied selenium concentration of 0.05 mg / L and a maintenance time of 36 hours, and the second stage having an applied selenium concentration of 0.20 mg / L and a maintenance time of 72 hours.
[0070] S5. The selenium-absorbing growing sprout body is subjected to stable growth environment regulation treatment to form selenium-rich pea sprout vegetables. The stable growth environment regulation treatment includes adjusting the growth environment parameters. The stable growth environment regulation treatment includes placing the selenium-absorbing growing sprout body in a periodic temperature environment with a day-night temperature difference of 12°C for 7 days. The daytime temperature of the periodic temperature environment is 23°C, the light time is 16 hours, and the nighttime temperature is 13°C. The relative humidity of the periodic temperature environment is 75%, and the carbon dioxide concentration is 1000 ppm.
[0071] Result analysis and effect: Germination rate: Under high temperature, long time, and high humidity conditions, the germination rate of pea seeds in the experimental group was 99%, which was about 14% higher than that of the traditional method. High humidity and high temperature promote uniform water absorption of seeds and increase germination rate.
[0072] Stability of selenium content: High humidity and high drug concentration treatment has strong stability of selenium content, uniform and stable absorption of selenium, and no excessive accumulation or toxicity inhibition of selenium in the experimental group for 7 days after germination.
[0073] Beneficial effects: Improved selenium conversion rate: The experimental group uses high temperature, long time, and high humidity treatment to ensure maximum conversion of selenium and improve its bioavailability.
[0074] Maintain high selenium content and food safety: High selenium content in the experimental group improves the nutritional value of selenium-rich pea sprout vegetables and avoids the negative effects of excessive selenium on plants, ensuring food safety.
[0075] Improved germination rate and growth efficiency: The experimental group of pea seeds treated under high humidity and high temperature conditions has a high germination rate, and the sprouts grow rapidly in the early stage without obvious inhibition.
[0076] Example 3 Please refer to Figures 2-3 On the basis of Example 1 and Example 2, a culture solution for selenium-rich pea sprout vegetables includes, by mass percentage: Nano-composite selenium particles: 0.05%.
[0077] Flavonoid photosensitive enhancer: 0.2%.
[0078] Seaweed extract: 0.2%.
[0079] Amino acid hydrolysate: 0.3%.
[0080] Sodium citrate: 0.1%.
[0081] Monopotassium phosphate: 0.1%.
[0082] Deionized water: 99.25%.
[0083] The carrier of the nanocomposite selenium particles is sodium carboxymethyl cellulose, and the average particle size of the nanocomposite selenium particles is 115 nm. The flavonoid photosensitive enhancer includes rutin and quercetin, and the mass ratio of rutin to quercetin is 3:4.
[0084] A method for biofortification of selenium-enriched pea sprouts, comprising: S1. Physiologically activating the pea seeds to form physiologically activated seeds. The steps of the physiologically activating treatment are: S11. Placing the pea seeds in warm water for preliminary activation to form first-stage treated seeds, the temperature of the warm water being 50°C, and the soaking time for preliminary activation being 12.5 minutes.
[0085] S12. Soaking the first-stage treated seeds in a gibberellin solution to form second-stage treated seeds, the concentration of the gibberellin solution being 75 mg / L, the soaking temperature being 27.5°C, and the soaking time being 4 hours.
[0086] S13. Rinsing the second-stage treated seeds with sterile water and filtering off the surface moisture to form the physiologically activated seeds, the number of rinsing times being 2.
[0087] S2. Contacting the physiologically activated seeds with a nanocomposite selenium source to form nanoseelenium-loaded seeds, the nanoseelenium-loaded seeds having a controllable release deposition layer, and the nanocomposite selenium source contacting treatment adding nanocomposite selenium particles. The particle size of the nanocomposite selenium particles is 115 nm, and the nanocomposite selenium particles adhere to the seed coat surface of the physiologically activated seeds to form a controllable release deposition layer.
[0088] S3. Photosensitive promoting treatment of the nanoseelenium-loaded seeds to form photoactivated selenium-transformed sprouts, the photosensitive promoting treatment including the addition of flavonoid photosensitive enhancers. The photosensitive promoting treatment includes placing the nanoseelenium-loaded seeds in a photobioreactor for culture, adding flavonoid photosensitive enhancers in the photobioreactor for photoreaction to form photoactivated selenium-transformed sprouts, the photoreaction light cycle being 16 hours of light and 8 hours of darkness, the photoreaction light intensity being 4500 Lux, the environmental humidity being 77.5%, and the culture time being 3 days. The photoreaction uses LED light sources, the LED light sources including red light and blue light, the red light wavelength being 660 nm±10 nm, the blue light wavelength being 450 nm±10 nm, and the light intensity ratio of red light to blue light being 7:3.
[0089] S4. The selenium absorption and growth sprout body is subjected to a phase control selenium treatment to form a selenium absorption and growth sprout body, and the phase control selenium treatment adopts a phase adjustment selenium supply mode. The phase adjustment selenium supply mode comprises a first phase and a second phase. The selenium concentration applied in the first phase is 0.03 mg / L, and the maintenance time is 30 hours. The selenium concentration applied in the second phase is 0.125 mg / L, and the maintenance time is 60 hours.
[0090] S5. The selenium absorption and growth sprout body is subjected to a growth environment stable regulation and control treatment to form a selenium-enriched pea sprout vegetable, and the growth environment stable regulation and control treatment comprises adjusting growth environment parameters. The growth environment stable regulation and control treatment comprises placing the selenium absorption and growth sprout body in a periodic temperature environment with a day-night temperature difference of 10℃ for 6 days. The daytime temperature of the periodic temperature environment is 22℃, the light time is 15 hours, and the nighttime temperature is 12℃. The relative humidity of the periodic temperature environment is 70%, and the carbon dioxide concentration is 900 ppm.
[0091] Result analysis and effect: Germination rate: Under the conditions of moderate temperature, short time and humidity, the germination rate of the pea seeds in the experimental group is 98%, which is 13% higher than that of the traditional method. Moderate humidity conditions effectively promote uniform water absorption of the seeds and improve the germination rate, avoiding the inhibition of excessive humidity on the sprouts.
[0092] Stability of selenium content: Through the treatment of moderate temperature and humidity, the selenium content in the culture solution is stable, and does not have adverse effects on the growth of the sprouts. The stability of selenium is guaranteed, and there is no excessive accumulation or inhibition of selenium in the experimental group 7 days after germination.
[0093] Beneficial effects: High conversion rate and stable selenium content: The use of moderate selenium concentration in the experimental group improves the conversion rate and maintains the uniform distribution of selenium in the plant, avoiding the toxic effects of high selenium content on plants.
[0094] Improved germination rate and growth efficiency: Through the treatment of moderate humidity and temperature in the experimental group, the germination rate and growth rate of the pea seeds are improved.
[0095] Energy saving and high efficiency: Through the treatment of moderate temperature and time, compared with the traditional high temperature treatment, energy is saved, and the conversion and absorption efficiency of selenium is higher.
[0096] Example 4 Please refer to Figure 3 On the basis of example 1, example 2 and example 3, by comparing different selenium-enriched culture solution formulations, biological strengthening methods under different time and temperature conditions, a basis is provided for producing different selenium content pea sprout vegetables.
[0097] Experimental group culture medium formula and bio-enhancement method Experimental group Nanocomposite selenium particle concentration Flavonoid photosensitive enhancer concentration Seaweed extract concentration Amino acid hydrolysate concentration Drug concentration Experimental group 1 0.02% 0.10% 0.10% 0.20% 0.05% Experimental group 2 0.08% 0.25% 0.30% 0.50% 0.20% Experimental group 3 0.05% 0.20% 0.20% 0.30% 0.10% Among them, the drug concentration includes sodium citrate, potassium dihydrogen phosphate.
[0098] Experimental group Selenium conversion treatment time Light intensity Photoperiod Temperature Humidity Selenium concentration Experimental group 1 3 days 4000 lux 16h light / 8h dark 21℃ 75% Low Experimental group 2 3 days 500 lux 16h light / 8h dark 23℃ 80% High Experimental group 3 3 days 4500 lux 16h light / 8h dark 22℃ 77% Medium 2. Experimental steps 2.1 Preparation of culture medium Each experimental group prepares the culture medium according to the formula in the above table.
[0099] 2 Seed treatment and inoculation According to the description of each experimental group, the pea seeds are respectively subjected to physiological activation treatment, and are soaked with the culture medium prepared respectively.
[0100] Experimental group 1: The experimental group uses a lower selenium concentration and a shorter soaking time. Experimental group 2: The experimental group uses a higher selenium concentration and a longer soaking time. Experimental group 3: The experimental group uses a medium selenium concentration and soaking time.
[0101] 3 Photosensitive promotion treatment The soaked seeds are placed in a photobioreactor for photosensitive promotion treatment.
[0102] Experimental group 1: The experimental group light intensity is 4000 lux, the environmental humidity is 75%, and the light cycle is 16 hours of light and 8 hours of darkness. Experimental group 2: The experimental group light intensity is 5000 lux, the environmental humidity is 80%, and the light cycle is 16 hours of light and 8 hours of darkness. Experimental group 3: The experimental group light intensity is 4500 lux, the environmental humidity is 77%, and the light cycle is 16 hours of light and 8 hours of darkness.
[0103] 4 Late treatment and growth After photosensitive promotion treatment, transplant to the corresponding culture environment for growth, and maintain temperature and humidity control.
[0104] Experimental group 1: The experimental group uses low selenium content and lower environmental humidity of 75% for growth.
[0105] Experimental group 2: The experimental group uses high selenium content and higher environmental humidity of 80% for growth.
[0106] Experimental group 3: The experimental group uses medium selenium content and medium humidity of 77% for growth.
[0107] Experimental group data and result analysis Germination rate: Experimental group 1: the germination rate of the experimental group is 96%, which is increased by 11% compared with the germination rate of the traditional method, the absorption of selenium is effective, and there is no growth inhibition. Experimental group 2: the germination rate of the experimental group is 99%, which is increased by about 14% compared with the germination rate of the traditional method, and high humidity and high selenium concentration effectively promote the water absorption and germination of seeds. Experimental group 3: the germination rate of the experimental group is 98%, which shows that the medium selenium concentration can keep good growth effect.
[0108] Selenium conversion rate: Experimental group 1: the selenium conversion rate of the experimental group is 85%, the conversion rate of selenium is high, and the selenium content is stable. Experimental group 2: the selenium conversion rate of the experimental group is 95%, the conversion rate is improved under high selenium concentration, which effectively promotes the absorption of selenium.
[0109] Experimental group 3: the selenium conversion rate of the experimental group is 90%, the conversion rate of selenium is high, and under the condition of moderate selenium concentration, the selenium content and conversion rate are well balanced.
[0110] Selenium stability: Experimental group 1: the treatment of low temperature and low humidity makes the absorption of selenium uniform, avoids excessive accumulation and toxic inhibition. Experimental group 2: the treatment of high humidity and high drug concentration makes the absorption of selenium more stable and uniform, the selenium content is higher, and no toxic reaction occurs. Experimental group 3: the treatment of moderate humidity and selenium concentration makes the selenium content stable, the absorption of selenium is uniform and has no negative effect on the growth of seedlings.
[0111] The above only describes some exemplary embodiments of the present application in a descriptive manner, and it is needless to say that the described embodiments can be modified in various ways without departing from the spirit and scope of the present application for those skilled in the art. Therefore, the above figures and descriptions are illustrative in nature and should not be understood as limiting the scope of protection of the claims of the present application.
Claims
1. A culture solution for selenium-enriched pea sprouts, comprising, characterized by, Consists of the following ingredients by mass percentage: Nano-composite selenium particles: 0.02%-0.08%; Flavonoid photosensitive enhancer: 0.10%-0.25%; Seaweed extract: 0.10%-0.30%; Amino acid hydrolysate: 0.20%-0.50%; Sodium citrate: 0.05%-0.20%; Potassium dihydrogen phosphate: 0.05%-0.20%; Deionized water: 98.5%-99.5%.
2. The selenium-enriched pea sprout according to claim 1, wherein the selenium-enriched pea sprout is characterized by: The carrier of the nano-composite selenium particles is sodium carboxymethyl cellulose, and the average particle size of the nano-composite selenium particles is 80nm-150nm.
3. The selenium-enriched pea sprout according to claim 1, wherein the selenium-enriched pea sprout is characterized by: The flavonoid photosensitive enhancer includes rutin and quercetin, and the mass ratio of the rutin to the quercetin is 3:
4.
4. A method for biofortifying selenium-rich pea sprouts, comprising the steps of: S1. Physiologically activating pea seeds to form physiologically activated seeds; S2. Contacting the physiologically activated seeds with a nano-composite selenium source to form nano-selenium-loaded seeds, the nano-selenium-loaded seeds having a controllable release deposition layer, and the nano-composite selenium source contacting treatment adding nano-composite selenium particles; S3. Light-sensitively promoting the nano-selenium-loaded seeds to form light-activated selenium-transformed sprouts, the light-sensitively promoting treatment including adding a flavonoid photosensitive enhancer; S4. Stage-regulating selenium supply to the light-activated selenium-transformed sprouts to form selenium-absorbing growing sprouts; S5. Stably regulating the growth environment of the selenium-absorbing growing sprouts to form selenium-rich pea sprouts, the stably regulating the growth environment including adjusting growth environment parameters.
5. The method of biofortification of selenium rich pea sprouts according to claim 4, wherein the method comprises the steps of: The step of the physiologically activating treatment is: S11. Placing the pea seeds in warm water for preliminary activation to form first-stage treated seeds, the temperature of the warm water being 45°C-55°C, and the soaking time of the preliminary activation being 10 minutes-15 minutes; S12. Soaking the first-stage treated seeds in a gibberellin solution to form second-stage treated seeds, the concentration of the gibberellin solution being 50mg / L-100mg / L, the soaking temperature being 25°C-30°C, and the soaking time being 3 hours-5 hours; S13. Rinsing the second-stage treated seeds with sterile water and filtering off surface moisture to form the physiologically activated seeds, the rinsing being performed 2-3 times.
6. The process as claimed in claim 4, wherein the selenium enriched pea sprout is biofortified by growing the seeds of the pea plant in the soil enriched with the selenium enriched yeast. 5 The particle size of the nano-composite selenium particles is 80nm-150nm, and the nano-composite selenium particles adhere to the seed coat surface of the physiologically activated seeds to form the controllable release deposition layer.
7. The process as claimed in claim 4, wherein the selenium enriched pea sprout is biofortified by growing the seeds of the pea plant in the soil containing the selenium enriched organic manure. 5 The light-sensitively promoting treatment includes culturing the nano-selenium-loaded seeds in a photobioreactor, adding the flavonoid photosensitive enhancer in the photobioreactor for photoreaction to form the light-activated selenium-transformed sprouts, the photoperiod of the photoreaction being 16 hours of light and 8 hours of darkness, the light intensity of the photoreaction being 4000Lux-5000Lux, the environmental humidity being 75%-80%, and the culturing time being 3 days.
8. The method of biofortification of selenium rich pea sprouts according to claim 7, wherein: The light reaction adopts an LED light source, the LED light source includes red light and blue light, the wavelength of the red light is 660nm±10nm, the wavelength of the blue light is 450nm±10nm, and the light intensity ratio of the red light to the blue light is 7:
3.
9. The biofortified selenium-enriched sprouted pea seedlings according to claim 4, characterized by: The selenium supply mode in the stage adjustment includes a first stage and a second stage, the selenium concentration applied in the first stage is 0.01mg / L-0.05mg / L, and the maintenance time is 24 hours-36 hours, the selenium concentration applied in the second stage is 0.05mg / L-0.20mg / L, and the maintenance time is 48 hours-72 hours.
10. The method of biofortification of selenium rich pea sprout as claimed in claim 4 wherein the method comprises of the steps of: The growth environment stable regulation and control treatment includes placing the selenium absorption and growth seedling body in a periodic temperature environment with a day-night temperature difference of 8℃-12℃ for 5 days-7 days, the daytime temperature of the periodic temperature environment is 22℃±1℃, the illumination time is 14 hours-16 hours, and the nighttime temperature is 12℃±1℃, and the relative humidity of the periodic temperature environment is 65%-75%, and the carbon dioxide concentration is 800ppm-1000ppm.