High-standard microbial flora for treating ammonia nitrogen and total phosphorus in large rivers and lakes and culture method

Abstract

The invention discloses a high-standard microbial flora for treating ammonia nitrogen and total phosphorus in large rivers and lakes and a culture method, the high-standard microbial flora comprises the following biological bacteria: photosynthetic bacteria, pseudogreen photosynthetic bacteria, bacillus amyloliquefaciens and proteolytic enzyme, and the photosynthetic bacteria and the pseudogreen photosynthetic bacteria have a synergistic effect to enhance the capability of decomposing organic matters in a water body; the bacillus amyloliquefaciens is responsible for decomposing starch substances in the water body, and the proteolytic enzyme is used for hydrolyzing proteins in the water body. According to the invention, multiple strains are reasonably combined and the proportion of the strains is optimized, so that pollutants are efficiently and synergistically removed; components of the culture medium are accurately matched, and proper nutrition is provided for microorganisms; culture conditions are accurately controlled in stages, and growth requirements of different microorganisms are met; culture parameters are monitored in real time and dynamically adjusted to ensure stable and efficient treatment of microorganisms; the collected concentrated microorganisms are convenient to store and transport and keep activity; the practical treatment effect is enhanced through adaptive domestication before application.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of microbial flora cultivation, in particular to a high-standard microbial flora for treating ammonia nitrogen and total phosphorus in large rivers, lakes and estuaries and a cultivation method thereof. BACKGROUND

[0002] In the ecological environment of large rivers, lakes and estuaries, excessive ammonia nitrogen and total phosphorus content is a serious problem that needs to be solved urgently. Excessive ammonia nitrogen can lead to water eutrophication, causing a large number of algae to reproduce and form water blooms or red tide phenomena. This not only consumes a large amount of dissolved oxygen in the water, causing fish and other aquatic organisms to die due to lack of oxygen, but also disrupts the ecological balance of the water body and affects the sustainable use of water resources. Excessive total phosphorus content is also an important inducement for water eutrophication, which can further exacerbate the growth of algae, leading to water quality deterioration and the emission of foul odors, causing great inconvenience to the surrounding environment and residents' lives.

[0003] Currently, there are various methods for treating ammonia nitrogen and total phosphorus in water bodies, among which microbial treatment technology has attracted widespread attention due to its green environmental protection, low cost and avoidance of secondary pollution. For example, the present technology disclosed in publication number CN120041303A, a microbial flora cultivation method for efficiently degrading organic pollutants, uses three consecutive cultivation stages of enrichment, domestication and optimization, precisely controlling parameters such as temperature, pH value, rotation speed and aeration rate in each stage to promote the growth of microorganisms with degradation ability. After multi-stage cultivation and domestication, the obtained microbial flora has high degradation ability for organic pollutants such as petroleum hydrocarbons, pesticides and polycyclic aromatic hydrocarbons, and strong adaptability to different concentrations of pollutants. However, this existing technology mainly focuses on the degradation of organic pollutants, and has certain limitations in terms of relevance and treatment effect for the widespread ammonia nitrogen and total phosphorus problems in large rivers, lakes and estuaries.

[0004] Some existing microbial treatment technologies often have problems such as unreasonable composition of microbial flora and insufficient synergistic effect between different strains when treating ammonia nitrogen and total phosphorus in large rivers, lakes and estuaries. Different microorganisms have different degradation abilities and adaptability to the environment, and if they are not reasonably matched, it is difficult to achieve efficient removal of ammonia nitrogen and total phosphorus. At the same time, during the cultivation of microorganisms, the composition and ratio of the culture medium have a huge impact on the growth and metabolism of microorganisms. The existing culture medium may not provide comprehensive and suitable nutrition for microorganisms, resulting in slow growth or low activity of microorganisms, which in turn affects the treatment effect of pollutants. In addition, the control of parameters such as temperature and dissolved oxygen concentration in the microbial cultivation conditions is not precise enough, and cannot meet the special needs of different microorganisms at different growth stages, limiting the overall treatment capacity of the microbial flora. SUMMARY

[0005] The present application aims to provide a high-standard microbial flora for treating ammonia nitrogen and total phosphorus in large rivers, lakes and ponds, and a culture method thereof, so as to solve the problems in the above background.

[0006] To achieve the above-mentioned purpose, the present application provides the following technical solution: a high-standard microbial flora for treating ammonia nitrogen and total phosphorus in large rivers, lakes and ponds, and a culture method thereof, comprising the following biological bacteria: photosynthetic-like bacteria, pseudomonas chlororaphis, bacillus amyloliquefaciens and protease; The photosynthetic-like bacteria and the pseudomonas chlororaphis work together to enhance the decomposition ability of organic matter in the water body, the bacillus amyloliquefaciens is responsible for decomposing starch substances in the water body, and the protease is used for hydrolyzing proteins in the water body, so as to jointly remove ammonia nitrogen and total phosphorus in large rivers, lakes and ponds.

[0007] Optionally, the proportion of each biological bacteria component in the microbial flora is optimized, wherein the proportion of photosynthetic-like bacteria is 20%-30%, the proportion of pseudomonas chlororaphis is 15%-25%, the proportion of bacillus amyloliquefaciens is 30%-40%, and the amount of protease is dynamically adjusted according to the protein content in the water body, so as to ensure comprehensive and efficient treatment of various pollutants.

[0008] The culture dish of the high-standard microbial flora for treating ammonia nitrogen and total phosphorus in large rivers, lakes and ponds comprises the high-standard microbial flora for treating ammonia nitrogen and total phosphorus in large rivers, lakes and ponds according to any one of the above, and the main components of the culture dish are fructose, glucose and bone meal; The fructose and glucose serve as carbon sources to provide energy for the microorganisms, and the bone meal serves as a source of nitrogen, phosphorus and trace elements to support the growth and metabolic activities of the microorganisms, and the components are mixed in a specific proportion to create a nutrient environment suitable for the growth of the microbial flora.

[0009] Optionally, the content of fructose in the culture dish is 10%-20%, the content of glucose is 15%-25%, and the content of bone meal is 30%-40%, and the rest is pure water or soft water. The above ratio has been verified by experiments to maximize the promotion of the reproduction of the microbial flora and the degradation ability of the pollutants.

[0010] The culture method of the high-standard microbial flora for treating ammonia nitrogen and total phosphorus in large rivers, lakes and ponds comprises the culture dish of the high-standard microbial flora for treating ammonia nitrogen and total phosphorus in large rivers, lakes and ponds according to any one of the above, and comprises the following steps: S1, inoculating microbial strains containing photosynthetic-like bacteria, pseudomonas chlororaphis, bacillus amyloliquefaciens and protease into a culture medium containing the components of the culture dish according to claim 3 or 4; S2, culturing under the condition of indoor temperature of 20-30 degrees; S3, the culture process is divided into the first three days of oxygen consumption stage and the subsequent anaerobic stage to adapt to the oxygen demand of different microorganisms in different growth periods.

[0011] Optionally, the first three days of oxygen consumption stage in S3 maintains the dissolved oxygen concentration in the culture environment at 2-4 mg / L by continuously supplying sterile air or oxygen to promote the rapid growth and reproduction of aerobic microorganisms such as photosynthetic-like bacteria and Bacillus amyloliquefaciens and enhance their decomposition ability of organic matter and starch in the water body.

[0012] Optionally, the subsequent anaerobic stage in S3 stops supplying oxygen and reduces the dissolved oxygen concentration in the culture environment to below 0.5 mg / L by sealing the culture container or using other anaerobic treatment means to facilitate the growth and metabolism of anaerobic microorganisms such as Pseudomonas chloroformis and promote the hydrolysis of protein in the water body by protein hydrolytic enzymes to achieve deep removal of ammonia nitrogen and total phosphorus.

[0013] Optionally, during the culture process of the microbial flora, the pH value, dissolved oxygen concentration, microbial quantity, and ammonia nitrogen and total phosphorus content of the culture solution are regularly detected, and the culture conditions are adjusted according to the detection results, such as adding nutrients, adjusting pH value, or changing aeration amount, to ensure that the microbial flora is always in the best growth state and efficient treatment state of pollutants.

[0014] Optionally, the culture method further comprises the steps of collecting and concentrating the mature microbial flora, separating the microbial flora from the culture solution by centrifugation, filtration or membrane separation, and concentrating to a certain concentration for storage, transportation and subsequent application in the treatment of ammonia nitrogen and total phosphorus in large rivers, lakes and rivers.

[0015] Optionally, the collected and concentrated microbial flora needs to be detected for activity and acclimated before being applied to the treatment of large rivers, lakes and rivers, and the microbial flora is cultured for a short period of time by simulating the environmental conditions of the target water body, such as temperature, pH value, dissolved oxygen concentration, etc., to improve its adaptability and treatment effect in the actual water body and ensure efficient and stable reduction of ammonia nitrogen and total phosphorus content in large rivers, lakes and rivers.

[0016] Compared with the prior art, the beneficial effects of the present application are as follows: 1. The microorganism group for treating high ammonia nitrogen and total phosphorus in large rivers, lakes and reservoirs and the culture method provided by the present application, by reasonably combining multiple strains and optimizing their proportions, efficient and synergistic removal of pollutants is achieved; the medium components are precisely matched to provide suitable nutrients for microorganisms; the culture conditions are precisely controlled in stages to meet the growth needs of different microorganisms; real-time monitoring and dynamic adjustment of culture parameters ensure stable and efficient treatment of microorganisms; concentrated microorganisms are collected for storage and transportation and maintain activity; adaptive acclimation before application enhances the actual treatment effect; the entire process is green, environmentally friendly and low-cost, avoiding secondary pollution, in line with the concept of sustainable development, and has high economic and environmental value.

[0017] 2. In the present application, by reasonably combining photosynthetic bacteria, pseudomonas chlororaphis, bacillus amyloliquefaciens and protease, the biological components play a synergistic role. Photosynthetic bacteria and pseudomonas chlororaphis synergistically enhance the decomposition ability of organic matter in water, bacillus amyloliquefaciens is responsible for decomposing starch in water, and protease hydrolyzes proteins in water. This multi-strain synergistic mode can comprehensively and efficiently treat various pollutants in large rivers, lakes and reservoirs, significantly reduce the content of ammonia nitrogen and total phosphorus in water, effectively improve water quality, and solve the problem of water eutrophication. Compared with the prior art, the microorganism group of the present application has more outstanding removal effect on ammonia nitrogen and total phosphorus, and can purify water more quickly and completely.

[0018] 3. In the present application, the proportions of each biological component in the microorganism group are optimized, wherein the proportion of photosynthetic bacteria is 20%-30%, the proportion of pseudomonas chlororaphis is 15%-25%, the proportion of bacillus amyloliquefaciens is 30%-40%, and the amount of protease added is dynamically adjusted according to the protein content in water. This precise proportioning is verified by a large number of experiments, which can ensure that each strain plays the best role in the microorganism group and realizes comprehensive and efficient treatment of various pollutants. Avoiding the problem of limited function or resource waste of some strains due to unreasonable strain ratio, the overall treatment efficiency and stability of the microorganism group are improved.

[0019] 4. In the present application, the main components are fructose, glucose and cow bone powder. Fructose and glucose as carbon source provide energy for microorganisms, and cow bone powder as nitrogen source and phosphorus source and trace element source supports the growth and metabolic activity of microorganisms. Each component is mixed according to a specific ratio, which has been verified by experiments to maximize the promotion of the reproduction of the microorganism group and the degradation ability of pollutants. Compared with the culture medium in the prior art, the culture medium of the present application is more in line with the nutritional needs of the microorganism group, providing a more comprehensive and suitable growth environment for microorganisms, which helps to improve the activity and treatment effect of microorganisms.

[0020] 5、The culture process is divided into an oxygen-consuming stage in the first three days and a subsequent anaerobic stage. In the oxygen-consuming stage in the first three days, by continuously supplying sterile air or oxygen, the dissolved oxygen concentration in the culture environment is maintained at 2-4 mg / L, promoting the rapid growth and reproduction of aerobic microorganisms such as photosynthetic-like bacteria and Bacillus amyloliquefaciens, and enhancing their decomposition capacity of organic matter and starch substances in the water body. In the subsequent anaerobic stage, oxygen supply is stopped, and the dissolved oxygen concentration in the culture environment is reduced to below 0.5 mg / L by sealing the culture container or using other anaerobic treatment means, which is beneficial to the growth and metabolism of anaerobic microorganisms such as Pseudomonas chloroacetica, and at the same time promotes the hydrolysis of proteins in the water body by protein hydrolytic enzymes, achieving deep removal of ammonia nitrogen and total phosphorus. This method of precise control of culture conditions in stages fully considers the oxygen demand of different microorganisms in different growth periods, provides the most suitable growth environment for microorganisms, and improves the treatment efficiency and stability of the microbial flora.

[0021] 6、In the present application, the pH value, dissolved oxygen concentration, microbial quantity, and the content of ammonia nitrogen and total phosphorus of the culture solution are detected regularly, and the culture conditions are adjusted according to the detection results, such as adding nutrients, adjusting pH value, or changing the aeration amount. This real-time monitoring and dynamic adjustment mechanism can ensure that the microbial flora is always in the best growth state and efficient treatment state of pollutants. Compared with the prior art, the culture method of the present application is more flexible and adaptable, and can timely respond to various changes in the culture process, ensuring the stable and reliable treatment effect of the microbial flora.

[0022] 7、The culture method of the present application also includes the steps of collecting and concentrating the mature microbial flora. The microbial flora is separated from the culture solution by centrifugation, filtration or membrane separation, and concentrated to a certain concentration. The volume of the concentrated microbial flora is reduced, which is convenient for storage and transportation, and reduces the storage and transportation cost. At the same time, the concentration process helps to maintain the activity of the microbial flora, reduces the activity loss in the storage and transportation process, and ensures that the microbial flora can exert the best effect when applied to the governance of large rivers, lakes and rivers. BRIEF DESCRIPTION OF DRAWINGS

[0023] Figure 1 The flow chart of the culture method of the high-standard microbial flora for treating ammonia nitrogen and total phosphorus in large rivers, lakes and rivers of the present application. DETAILED DESCRIPTION

[0024] The technical solutions in the embodiments of the present application will be described clearly and completely below with reference to the drawings in the embodiments of the present application. Obviously, the described embodiments are only part of the embodiments of the present application, not all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative labor are within the scope of protection of the present application.

[0025] Embodiment 1 Please refer to Figure 1 , the high-standard microbial flora for treating ammonia nitrogen and total phosphorus in large rivers, lakes and ponds, and a culture method thereof, comprising the following biological bacteria: photosynthetic-like bacteria, pseudomonas chlororaphis, bacillus amyloliquefaciens and protein hydrolytic enzyme; In order to achieve efficient removal of ammonia nitrogen and total phosphorus in large rivers, lakes and ponds, in the embodiment: photosynthetic-like bacteria and pseudomonas chlororaphis work together, and the two complement each other, significantly enhancing the decomposition ability of organic matter in water; bacillus amyloliquefaciens is responsible for decomposing starch substances in water, effectively reducing the content of starch pollutants in water; protein hydrolytic enzyme hydrolyzes protein in water, decomposing it into small molecules. Through the joint action of these biological bacteria, ammonia nitrogen and total phosphorus in large rivers, lakes and ponds can be comprehensively and efficiently removed, and water quality can be improved.

[0026] In order to ensure comprehensive and efficient treatment of various pollutants, in the embodiment: the proportions of each biological bacteria component in the microbial flora are optimized. Among them, the proportion of photosynthetic-like bacteria is 20%-30%, the proportion of pseudomonas chlororaphis is 15%-25%, the proportion of bacillus amyloliquefaciens is 30%-40%, and the amount of protein hydrolytic enzyme is dynamically adjusted according to the protein content in water. This precise proportioning makes each biological bacteria fully play its role, avoids the problem of poor treatment effect caused by improper proportioning, and thus realizes targeted treatment of different types of pollutants and improves the overall treatment efficiency.

[0027] Embodiment 2 Please refer to Figure 1 , the culture dish of the high-standard microbial flora for treating ammonia nitrogen and total phosphorus in large rivers, lakes and ponds, comprising the high-standard microbial flora for treating ammonia nitrogen and total phosphorus in large rivers, lakes and ponds described in Embodiment 1, and the main components of the culture dish are fructose, glucose and bone meal; In order to provide a suitable growth and metabolism environment for microorganisms, in the embodiment: fructose and glucose are used as carbon sources to provide necessary energy for microorganisms and support their life activities; bone meal is used as a source of nitrogen, phosphorus and trace elements to meet the needs of microbial growth for various nutrients. Each component is mixed according to a specific proportion to create a nutrient environment suitable for the growth of microbial flora, promote the reproduction and metabolism of microorganisms, and thus enhance their degradation ability of pollutants.

[0028] In order to maximize the reproduction of microbial flora and the degradation capacity of pollutants, in this embodiment: the content of each component in the culture dish is precisely controlled. Among them, the content of fructose is 10%-20%, the content of glucose is 15%-25%, the content of bovine bone powder is 30%-40%, and the rest is pure water or soft water. This ratio has been verified through a large number of experiments, which can provide the most suitable nutrient conditions for microorganisms, so that the microorganisms can maintain the best growth state during the cultivation process, thereby improving their degradation efficiency of ammonia nitrogen and total phosphorus.

[0029] Embodiment 3 Please refer to Figure 1 , the culture method of high-standard microbial flora for treating ammonia nitrogen and total phosphorus in large rivers, lakes and rivers, including the culture dish for treating ammonia nitrogen and total phosphorus in large rivers, lakes and rivers described in embodiment 2, characterized in that it comprises the following steps: S1, the microbial strain containing photosynthetic-like bacteria, pseudomonas chloroformis, bacillus amyloliquefaciens and proteinase is inoculated into the culture medium containing the components of the culture dish of claim 3 or 4; S2, cultivate under the condition of indoor temperature 20-30 degrees; S3, the cultivation process is divided into the first three days of oxygen consumption stage and the subsequent anaerobic stage to adapt to the oxygen demand of different microorganisms in different growth periods.

[0030] In order to promote the rapid growth and reproduction of aerobic microorganisms such as photosynthetic-like bacteria and bacillus amyloliquefaciens, and enhance their decomposition capacity of organic matter and starch substances in water body, in this embodiment: in the first three days of oxygen consumption stage in S3, sterile air or oxygen is continuously introduced to maintain the dissolved oxygen concentration in the culture environment at 2-4 mg / L. Under this condition, aerobic microorganisms can fully utilize oxygen for aerobic respiration, rapid growth and metabolism, thereby more effectively decomposing organic matter and starch substances in water body, laying a good foundation for subsequent pollution treatment.

[0031] In order to facilitate the growth and metabolism of anaerobic microorganisms such as pseudomonas chloroformis, and at the same time promote the hydrolysis of proteinase to protein in water body, and achieve deep removal of ammonia nitrogen and total phosphorus, in this embodiment: in the subsequent anaerobic stage of S3, oxygen is stopped and the dissolved oxygen concentration in the culture environment is reduced to below 0.5 mg / L by sealing the culture container or using other anaerobic treatment means. In this anaerobic environment, anaerobic microorganisms can better exert their metabolic function, and proteinase can more effectively hydrolyze protein, further reducing the content of ammonia nitrogen and total phosphorus in water body, achieving the purpose of deep treatment.

[0032] In order to ensure that the microbial flora is always in the best growth state and efficient treatment state of pollutants, in this embodiment: during the cultivation of the biological flora, the pH value, dissolved oxygen concentration, microbial quantity, and the content of ammonia nitrogen and total phosphorus of the culture solution are detected regularly, and the culture conditions are adjusted according to the detection results, such as adding nutrients, adjusting pH value or changing aeration amount. Through this dynamic adjustment, problems that occur in the cultivation process can be solved in time, the most suitable growth environment for the microorganisms is provided, and the efficient treatment capacity of the microorganisms on pollutants is ensured.

[0033] In order to facilitate storage, transportation and subsequent application to the treatment of ammonia nitrogen and total phosphorus in large rivers, lakes and seas, in this embodiment: the culture method also includes the steps of collecting and concentrating the mature microbial flora. The microbial flora is separated from the culture solution by centrifugation, filtration or membrane separation, and concentrated to a certain concentration. In this way, not only the preservation and transportation of the microbial flora are facilitated, but also the use efficiency is improved and the treatment effect on pollutants is enhanced in subsequent application.

[0034] In order to ensure that the microbial flora collected and concentrated can efficiently and stably reduce the content of ammonia nitrogen and total phosphorus in large rivers, lakes and seas in actual water bodies, in this embodiment: before being applied to the treatment of large rivers, lakes and seas, the microbial flora collected and concentrated needs to be detected for activity and adapted. By simulating the environmental conditions of the target water body, such as temperature, pH value, dissolved oxygen concentration, etc., the microbial flora is cultured for a short period of time, so that it gradually adapts to the environment of the target water body, and the adaptability and treatment effect in actual water bodies are improved.

[0035] In use: first, by reasonably configuring biological bacteria such as photosynthetic bacteria, pseudomonas chlororaphis, bacillus amyloliquefaciens and protease, a microbial flora with high degradation capacity is formed. Then, using a culture dish with specific ingredients and proportions, a suitable nutrient environment is provided for the microorganisms, and they are cultured at a suitable temperature. During the cultivation process, the dissolved oxygen concentration of the culture environment is controlled in stages according to the oxygen demand of different microorganisms, to promote the growth and metabolism of aerobic and anaerobic microorganisms. At the same time, by regularly detecting and adjusting the culture conditions, the microbial flora is ensured to be always in the best state. Finally, the mature microbial flora collected and concentrated is detected for activity and adapted, so that it can better adapt to the actual water body environment, efficiently and stably reduce the content of ammonia nitrogen and total phosphorus in large rivers, lakes and seas, and realize effective treatment of the water body.

[0036] Although embodiments of the present application have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made therein without departing from the principles and spirit of the application, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A microbial flora for treating ammonia nitrogen and total phosphorus in large rivers, lakes and seas, characterized in that: The biological bacteria include the following: ​ Photosynthetic-like bacteria, pseudomonas chlororaphis, bacillus amyloliquefaciens, and proteolytic enzymes; The photosynthetic-like bacteria and pseudomonas chlororaphis work together to enhance the decomposition ability of organic matter in the water body, the bacillus amyloliquefaciens is responsible for decomposing starch substances in the water body, and the proteolytic enzyme hydrolyzes proteins in the water body, together realizing the removal of ammonia nitrogen and total phosphorus in large rivers, lakes, and other water bodies.

2. The microorganism colony according to claim 1, wherein the microorganism colony is characterized in that: The proportion of each biological bacteria component in the microbial flora is optimized, wherein the proportion of photosynthetic-like bacteria is 20%-30%, the proportion of pseudomonas chlororaphis is 15%-25%, the proportion of bacillus amyloliquefaciens is 30%-40%, and the amount of proteolytic enzyme is dynamically adjusted according to the protein content in the water body to ensure comprehensive and efficient treatment of various pollutants.

3. The Petri dish of the high-standard microbial flora for treating ammonia nitrogen and total phosphorus in large rivers, lakes and seas, comprising the high-standard microbial flora for treating ammonia nitrogen and total phosphorus in large rivers, lakes and seas according to any one of claims 1-2, characterized in that: The main components of the culture dish are fructose, glucose, and cow bone powder; Fructose and glucose serve as carbon sources to provide energy for microorganisms, and the cow bone powder serves as a nitrogen source, a phosphorus source, and a source of trace elements to support the growth and metabolic activities of microorganisms. Each component is mixed in a specific proportion to create a nutrient environment suitable for the growth of microbial flora.

4. The petri dish for culturing the high-standard microbial flora for treating high ammonia nitrogen and total phosphorus in large rivers, lakes and seas according to claim 3, characterized in that: The content of fructose in the culture dish is 10%-20%, the content of glucose is 15%-25%, and the content of cow bone powder is 30%-40%. The rest is pure water or soft water. This ratio has been experimentally verified to maximize the reproduction of microbial flora and the degradation ability of pollutants.

5. The culture method of the high-standard microbial flora for treating ammonia nitrogen and total phosphorus in large rivers, lakes and seas, comprising the culture dish of the high-standard microbial flora for treating ammonia nitrogen and total phosphorus in large rivers, lakes and seas according to any one of claims 3-4, characterized in that: The method comprises the following steps: S1. The microbial species containing photosynthetic-like bacteria, pseudomonas chlororaphis, bacillus amyloliquefaciens, and proteolytic enzymes are inoculated into a culture medium containing the components of the culture dish of claim 3 or 4; S2. Culturing is carried out at a room temperature of 20-30 degrees; S3. The culturing process is divided into an oxygen-consuming stage for the first three days and a subsequent anaerobic stage to adapt to the oxygen demand of different microorganisms at different growth stages.

6. The culture method of the high-standard microbial flora for treating ammonia nitrogen and total phosphorus in large rivers, lakes and seas according to claim 5, characterized in that: In the first three days of the oxygen-consuming stage in S3, sterile air or oxygen is continuously introduced to maintain the dissolved oxygen concentration in the culture environment at 2-4 mg / L to promote the rapid growth and reproduction of aerobic microorganisms such as photosynthetic-like bacteria and bacillus amyloliquefaciens, and to enhance their decomposition ability of organic matter and starch substances in the water body.

7. The method according to claim 6, wherein the method is characterized by the following steps: 1) inoculating the culture medium with the high-standard microbial flora; 2) culturing the high-standard microbial flora in the culture medium; 3) collecting the high-standard microbial flora; and 4) storing the high-standard microbial flora. In the subsequent anaerobic stage in S3, oxygen supply is stopped, and the culture container is sealed or other anaerobic treatment means are used to reduce the dissolved oxygen concentration in the culture environment to below 0.5 mg / L to facilitate the growth and metabolism of anaerobic microorganisms such as pseudomonas chlororaphis, and to promote the hydrolysis of proteins in the water body by proteolytic enzymes, achieving deep removal of ammonia nitrogen and total phosphorus.

8. The culture method of the high-standard microbial flora for treating ammonia nitrogen and total phosphorus in large rivers, lakes and seas according to any one of claims 5-7, characterized in that: During the cultivation of the biological bacteria flora, the pH value, dissolved oxygen concentration, microbial quantity, and the contents of ammonia nitrogen and total phosphorus in the culture solution are regularly detected, and the culture conditions are adjusted according to the detection results, such as adding nutrients, adjusting the pH value, or changing the aeration amount, to ensure that the microbial flora is always in the best growth state and efficient treatment state of pollutants.

9. The microorganism group and culture method for treating high ammonia nitrogen and total phosphorus in large rivers, lakes and seas according to any one of claims 5-8, characterized in that: The culture method further comprises the steps of collecting and concentrating the cultured mature microbial flora, separating the microbial flora from the culture solution by technical means such as centrifugation, filtration or membrane separation, and concentrating to a certain concentration, so as to facilitate storage, transportation and subsequent application in the treatment of ammonia nitrogen and total phosphorus in large rivers, lakes and seas.

10. The microbial flora and culture method for treating high ammonia nitrogen and total phosphorus in large rivers, lakes and seas according to claim 9, characterized in that: The collected and concentrated microbial flora still needs to be detected for activity and adapted before being applied to the treatment of large rivers, lakes and seas, and the microbial flora is cultured for a short period of time by simulating the environmental conditions of the target water body, such as temperature, pH value, dissolved oxygen concentration, etc., so as to improve its adaptability and treatment effect in the actual water body, and ensure that the content of ammonia nitrogen and total phosphorus in large rivers, lakes and seas can be efficiently and stably reduced.

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