Chemiluminescence detection kit based on nucleic acid aptamer and preparation method thereof

By using nucleic acid aptamers and an improved buffer formulation, the prepared chemiluminescence detection kit solves the problems of high antibody cost and poor stability, achieving chemiluminescence detection with higher sensitivity and a wider detection range.

CN121364306APending Publication Date: 2026-01-20HANGZHOU AIPJIAZHEN BIOTECHNOLOGY CO LTD +1
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Patent Information

Application Number
CN202511060989.X
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2024-07-18
Publication Date
2026-01-20

AI Technical Summary

Technical Problem

Existing magnetic microparticle chemiluminescence methods suffer from high antibody production costs, poor stability, and significant batch-to-batch variations, limiting the application of immunoassay reagents. There is a lack of magnetic microparticle chemiluminescence detection methods based on nucleic acid aptamers.

Method used

Nucleic acid aptamers were used as recognition molecules, combined with chemiluminescent labels, to prepare reagents M and R. The stability and sensitivity of the reagents were ensured by using an improved buffer formulation, including FA-CS-SOD and ethyl undecanoate raffinose.

Benefits of technology

This improved the stability and sensitivity of the chemiluminescence detection kit, expanded the range of detectable substances, reduced production costs, and improved detection accuracy and stability.

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Abstract

The invention belongs to the field of medical detection, and mainly relates to a chemiluminiscence detection kit based on a nucleic acid aptamer and a preparation method of the chemiluminiscence detection kit. The chemiluminiscence detection kit prepared by the invention comprises reagents M and R. The reagent M comprises a magnetic particle coupling recognition molecule which can be called as a capture probe; the reagent R comprises a chemiluminescent marker coupled recognition molecule which can be called as a chemiluminescent probe; the recognition molecules are aptamers or antibodies, and the recognition molecules in the reagent M and the reagent R are not antibodies at the same time. The chemiluminescence detection kit combines convenient detection and analysis of a magnetic particle chemiluminescence method and high specificity and high affinity of the aptamer, and can timely and accurately detect various proteins. Different from a capture probe and a chemiluminescent probe which are constructed by using a protein antibody in the market, the kit overcomes the problems of poor stability, difficulty in modification, high production cost and the like of the protein antibody, and is of great significance in the field of immunodetection.
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