Method for regulating soilless low-temperature vernalization and arrow drawing of cymbidium goeringii

Abstract

The invention provides a method for regulating and controlling soilless low-temperature vernalization and arrow drawing of cymbidium goeringii, and belongs to the technical field of cymbidium goeringii flowering phase regulation and control. The method comprises the following steps: (1) washing the root system of cymbidium goeringii, and airing to separate out plants with flower buds; (2) loading the plant root with the flower bud into a container containing a culture medium; (3) wrapping with a buffer product; (4) placing the plants in a refrigeration house to complete vernalization; and (5) spraying a plant growth regulator, wrapping the buffer product, and then transferring into an environment with the day temperature of 23-27 DEG C and the night temperature of 13-17 DEG C for flower forcing culture, so that the plant is subjected to arrow drawing, namely the vernalization and arrow drawing process of cymbidium goeringii is completed. According to the method for soilless low-temperature vernalization of the cymbidium goeringii and growth promotion of the arrows, the flowering period of the cymbidium goeringii is manually regulated and controlled, out-of-season or timed flowering of the cymbidium goeringii is achieved, the production cost is reduced, the efficiency is improved, and meanwhile the commodity value and market adaptability of the cymbidium goeringii are improved.

Description

TECHNICAL FIELD

[0001] The present application relates to the flowering regulation technology field of Cymbidium goeringii, in particular to a method for regulating the soilless low-temperature vernalization and arrow extraction of Cymbidium goeringii. BACKGROUND

[0002] Cymbidium goeringii is a perennial herb of Orchidaceae and Cymbidium, also known as one-stem-one-flower, grass orchid, mountain orchid and Doudaoxiang. Cymbidium goeringii is the main representative of Chinese orchids, and is deeply loved by the Chinese people because of its pure and elegant, fragrant and remote. Huang Tingjian of the Song Dynasty once said: "one stem and one flower are called orchid, and one stem and many flowers are called Hui." Since then, orchid and Hui have been named. The leaf color of Cymbidium goeringii is yellow-green and dark green, and the flower color is light yellow-green, with pure fragrance. Cymbidium goeringii is one of the most widely distributed species in Cymbidium plants in China, and the varieties and yield of Cymbidium goeringii in Jiangsu and Zhejiang 300-500 meters and Yunnan 800-1600 meters are the most abundant.

[0003] The flower bud of Cymbidium goeringii emerges in late July to mid-August, and stops growing after growing about 2-3 cm. It needs a period of low temperature in winter for vernalization, so as to bloom with good fragrance. The natural flowering period of Cymbidium goeringii is from February to March, and the flowering period is about one month. The market competition is not strong compared with the orchid which provides flowering products throughout the year. The conventional method for vernalization treatment of Cymbidium goeringii is to control the temperature in the greenhouse where the orchid grows to about 4℃ for 30-45 days, and to support the management of greenhouse facilities, humidity and light. However, due to the large space of the greenhouse, the artificial regulation of vernalization is often limited by high energy consumption and other problems. Cymbidium goeringii can also be potted in a cold storage to regulate the flowering period, but the potting substrate is prone to water loss in the cold storage, and the potted Cymbidium goeringii occupies a large area and is difficult to stack, making it difficult to carry out large-scale regulation treatment. Therefore, it is necessary to develop a high-efficiency and feasible technology for regulating the vernalization and flowering time of Cymbidium goeringii. SUMMARY

[0004] Therefore, the present application provides a method for regulating the flowering time of Cymbidium goeringii, which has low technical difficulty and simple operation, can effectively improve the flexibility of the flowering time of Cymbidium goeringii, and has important significance for improving the commodity nature of Cymbidium goeringii and promoting the industrial development.

[0005] In order to achieve the above purpose, the present application adopts the following technical scheme:

[0006] The present application provides a method for regulating the soilless low-temperature vernalization and arrow extraction of Cymbidium goeringii, comprising the following steps:

[0007] (1) The roots of Cymbidium goeringii are washed clean, dried and divided into plants with flower buds, which are prepared for use;

[0008] (2) The roots of the plants with flower buds are placed in a container containing agar medium;

[0009] (3) The plant obtained in step (2) is wrapped with buffer product;

[0010] (4) The wrapped plant is stored in an environment with a temperature of 3-5°C for 30-45 days, and the plant is vernalized;

[0011] (5) The plant is vernalized, and the plant growth regulator is sprayed on the surface of the leaf of the plant, and then the plant is wrapped with buffer product and transferred to an environment with a day temperature of 23-27°C and a night temperature of 13-17°C for 12-17 days of flower induction culture, and the plant is completed arrow extraction, that is, the vernalization and arrow extraction process of the Cymbidium is completed, and the plant is transplanted or sold according to needs.

[0012] Further, the Cymbidium in step (1) is a potted Cymbidium with flower buds.

[0013] Further, the agar culture medium in step (2) is not sterilized at high temperature, and a certain concentration of antibiotic and bactericide are added to the agar culture medium. The concentration of the antibiotic is 0.2-0.5 g / L, and the antibiotic is selected from any one of penicillin, penicillin potassium, and penicillin sodium, preferably the antibiotic is penicillin. The concentration of the bactericide is 0.2-0.5 g / L, and the bactericide is selected from any one of hymexazol powder, hymexazol water, and hymexazol emulsion, preferably the bactericide is hymexazol powder.

[0014] Further, the agar culture medium in step (2) comprises the following components:

[0015] Culture medium+4 million units of penicillin, 98% hymexazol, sodium benzoate, sucrose, agar powder, and activated carbon, wherein the concentration of penicillin is 0.2-0.5 g / L, the concentration of 98% hymexazol is 0.2-0.5 g / L, the concentration of sodium benzoate is 0.05-0.1 g / L, the concentration of sucrose is 15-25 g / L, the concentration of agar powder is 3.0-4.0 g / L, and the concentration of activated carbon is 1 g / L.

[0016] Further, the preparation method of the agar culture medium in step (2) comprises the following steps: weighing the culture medium according to the formula and dissolving it in tap water; adding sucrose and activated carbon in sequence, stirring uniformly, adjusting the pH value to 5.1-5.4, adding agar powder, and stopping heating after stirring and heating to 100°C; when the temperature of the culture medium falls to 50-45°C, penicillin, hymexazol, and sodium benzoate are added in sequence according to the formula, and the agar culture medium is obtained after stirring uniformly.

[0017] Further, the pH of the culture medium is 5.1-5.4.

[0018] Further, after the plant with flower buds is placed in the container containing the agar culture medium in step (2), the plant is bundled at the pseudobulb.

[0019] Further, the buffer product in step (3) is an air-filled bag, preferably, the buffer product is an air-filled column bag.

[0020] Further, the spraying concentration of the plant growth regulator in step (5) is 60-90 mg / L.

[0021] Further, the plant growth regulator in step (5) includes gibberellin, preferably, the plant growth regulator is gibberellin GA3.

[0022] Further, the spraying part of the plant growth regulator is the front and back of the plant, and the spraying is preferably to the extent of uniform moisture without dripping water.

[0023] Compared with the prior art, the present application has the following beneficial effects:

[0024] The method provided by the present application carries out soilless low-temperature vernalization and promotes the growth of Cymbidium flowers, controls the flowering period of Cymbidium by artificial regulation, realizes off-season or timed flowering of Cymbidium, reduces production cost, improves efficiency, and also improves the commodity value and market adaptability of Cymbidium.

[0025] The present application uses a bagged agar medium to replace conventional substrate soil, which can provide growth nutrients for plants.

[0026] The present application wraps the plant washed by soil removal with a buffer material, which can provide stable vernalization for the plant, and the buffer material, such as an air-filled column bag, has a light weight, a good protection effect on the plant and flower bud, and can be stacked, greatly improving the space utilization rate of the cold storage, and solving the problems of inaccurate regulation and control, low space utilization rate, high energy consumption, and unstable product quality of the plant after regulation and control in conventional vernalization treatment.

[0027] The culture medium used in the present application does not need high-temperature sterilization, and the addition of antibiotics and bactericides and other components in the culture medium plays a role in preventing and controlling the growth of miscellaneous bacteria. BRIEF DESCRIPTION OF DRAWINGS

[0028] Figure 1 is a potting picture of Cymbidium with unbroken flower buds in Example 1 of the present application.

[0029] Figure 2 is a plant picture of Cymbidium after soil removal and root cleaning in Example 1 of the present application.

[0030] Figure 3 is a plant picture of Cymbidium with flower buds in Example 1 of the present application.

[0031] Figure 4 is a picture of an agar culture bag configured in Example 1 of the present application.

[0032] Figure 5 is the picture of the Cymbidium plant roots packed in the agar culture bag, and the culture medium sticks to the surface of the plant roots in Example 1 of the present application.

[0033] Figure 6 is the picture of the Cymbidium plant wrapped in the inflation plant bag for cold storage vernalization in Example 1 of the present application.

[0034] Figure 7 is the picture of the Cymbidium plant flower forcing regulation in Example 1 of the present application.

[0035] Figure 8 is the picture of the Cymbidium plant arrow extraction and flowering after flower forcing in Example 1 of the present application. DETAILED DESCRIPTION

[0036] The present application provides a method for regulating Cymbidium soilless low-temperature vernalization and arrow extraction, comprising the following steps:

[0037] (1) washing the Cymbidium roots clean, drying and separating the plants with flower buds, for standby;

[0038] (2) packing the roots of the plants with flower buds into a container containing agar culture medium;

[0039] (3) wrapping the plants obtained in step (2) with buffer products;

[0040] (4) placing the wrapped plants in an environment of 3-5℃ for 30-45 days, and the plants complete vernalization;

[0041] (5) spraying plant growth regulators on the surface of the leaves of the plants that have completed vernalization, wrapping with buffer products, and then transferring to an environment of day temperature 23-27℃ and night temperature 13-17℃ for 12-17 days of flower forcing culture, and the plants complete arrow extraction, i.e. the vernalization and arrow extraction process of Cymbidium is completed, and the plants are transplanted or sold as needed.

[0042] In some embodiments of the present application, the Cymbidium in step (1) is a potted Cymbidium with flower buds out of the soil, preferably the Cymbidium in step (1) is a potted Cymbidium with flower buds out of the soil after September, ensuring that the flower buds have completed flower bud differentiation.

[0043] The present application can solve the problems of large land occupation and high energy consumption of traditional potted Cymbidium by the steps of washing the Cymbidium roots to remove soil and separating the plants with flower buds, which helps to realize large-scale Cymbidium vernalization treatment.

[0044] In some embodiments of the present application, the agar culture medium in step (2) is not sterilized by high temperature, and a certain concentration of antibiotic and bactericide is added in the agar culture medium, the concentration of the antibiotic is 0.2-0.5 g / L, the antibiotic is selected from any one of penicillin, penicillin potassium, and penicillin sodium, and preferably, the antibiotic is penicillin. The concentration of the bactericide is 0.2-0.5 g / L, the bactericide is selected from any one of hymexazol powder, hymexazol water, and hymexazol emulsion, and preferably, the bactericide is hymexazol powder.

[0045] In some embodiments of the present application, the agar culture medium in step (2) comprises the following components:

[0046] The culture medium + 4 million units of penicillin, 98% hymexazol, sodium benzoate, sucrose, agar powder, and activated carbon, wherein the concentration of penicillin is 0.2-0.5 g / L, the concentration of 98% hymexazol is 0.2-0.5 g / L, the concentration of sodium benzoate is 0.05-0.1 g / L, the concentration of sucrose is 15-25 g / L, the concentration of agar powder is 3.0-4.0 g / L, and the concentration of activated carbon is 1 g / L. The appropriate concentration of agar powder can ensure that the culture medium is in a viscous state, can be uniformly adhered to the plant roots by extrusion, and can provide sufficient nutrients for the plant.

[0047] In some embodiments of the present application, the preparation method of the agar culture medium in step (2) comprises the following steps: weighing the culture medium according to the formula and dissolving it in tap water; adding sucrose and activated carbon in sequence, stirring uniformly, adjusting the pH value to 5.1-5.4, adding agar powder, and stopping heating after stirring and heating to 100°C; when the temperature of the culture medium falls to 50-45°C, adding penicillin, hymexazol, and sodium benzoate according to the formula in sequence, and stirring uniformly to obtain the agar culture medium.

[0048] In some embodiments of the present application, the culture medium is selected from any one of 1 / 2MS medium, KC medium, and Huobao No. 1 medium.

[0049] In some embodiments of the present application, the agar culture medium is poured into a plastic bag after cooling to 45°C, and the agar culture medium is in a dilute gel state after normal temperature.

[0050] In some embodiments of the present application, the pH of the culture medium is 5.1-5.4.

[0051] In some embodiments of the present application, after the plant with flower buds is placed in the container containing the agar culture medium in step (2), the plant is tied at the pseudobulb. Preferably, the plant is tied at the pseudobulb with an elastic band or a rope, which can prevent the root system from sliding in the bag, avoid the diffusion and waste of the culture medium, and protect the growth and elongation of the flower buds.

[0052] The container in step (2) is not particularly limited in type or material, and any conventional container capable of containing nutrient agar medium can be used in the present application. Preferably, the container in step (2) is a plastic bag, which can contain the root system of Cymbidium.

[0053] In some embodiments of the present application, the plant with flower buds is placed in a container containing agar medium in step (2), and the agar is squeezed to evenly coat the plant roots, simulating plant tissue culture. The medium can ensure that the plant maintains normal physiological growth needs during the process of vernalization and promoting flower arrow growth in the cold storage, prevent the growth of miscellaneous bacteria, and reduce the damage to the plant caused by the control process.

[0054] In some embodiments of the present application, the buffer product in step (3) is an air-filled bag, preferably an air-filled column bag. The length of the air-filled column bag can wrap the whole Cymbidium plant, avoiding the exposure of plant leaves. Further preferably, the air-filled column bag is used to wrap the plants in sequence, and the plants wrapped in the air-filled column bag are placed in the cold storage.

[0055] In the present application, the air-filled bag can effectively buffer the invasion of cold air and hot air on the plant during the process of vernalization and flower arrow promotion, making the environmental temperature change of the plant more stable, and further protecting the flower buds so that they can grow normally without being squeezed. The plant can also be better protected when delivered as a commodity.

[0056] In some embodiments of the present application, compared with the flowering period of Cymbidium in late February and early March under natural conditions, the plant with flower buds emerging in September is placed in a cold storage at 3-5°C for 30-45 days, and then the flower can be promoted at any time, providing flowering Cymbidium products for the market and improving the flexibility of Cymbidium flowering time and the commodity nature of Cymbidium.

[0057] In some embodiments of the present application, the spraying concentration of the plant growth regulator in step (5) is 60-90 mg / L, preferably 60 mg / L.

[0058] In some embodiments of the present application, the plant growth regulator in step (5) includes gibberellin, preferably GA3.

[0059] In some embodiments of the present application, the spraying site of the plant growth regulator is the front and back of the plant, and the spraying should be uniform, moist, and not dripping.

[0060] Artificial vernalization and flower promotion can improve the quality of Cymbidium, avoiding short and deformed flower branches.

[0061] In order to make the objects, technical solutions and advantages of the present application clearer, the technical solutions of the present application will be clearly and completely described below. Obviously, the described embodiments are only some of the embodiments of the present application, but not all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative work fall within the scope of protection of the present application.

[0062] In the following examples, the test methods or test methods are conventional methods unless otherwise specified; the raw materials and adjuvants are obtained from conventional commercial channels or prepared by conventional methods unless otherwise specified.

[0063] The composition of the agar medium in step (2) in the following examples is as follows:

[0064] 1 / 2 MS medium + 4 million units of penicillin, 98% hymexazol, sodium benzoate, sucrose, agar powder and activated carbon, wherein the concentration of penicillin is 0.2-0.5 g / L, the concentration of 98% hymexazol is 0.2-0.5 g / L, the concentration of sodium benzoate is 0.05-0.1 g / L, the concentration of sucrose is 15-25 g / L, the concentration of agar powder is 3.0-4.0 g / L, the concentration of activated carbon is 1 g / L, and the pH value is 5.1-5.4.

[0065] The preparation method of the agar medium in step (2) is as follows:

[0066] According to the formula, 1 / 2 MS medium dry powder is weighed and put into tap water to dissolve the medium dry powder; sucrose and activated carbon are added in turn, and the pH value is adjusted to 5.1-5.4 after stirring uniformly; agar powder is added, and stirring and heating are stopped when the temperature reaches 100℃; when the medium temperature falls to 45℃, penicillin, hymexazol and sodium benzoate are added in turn according to the formula, and stirred uniformly to obtain the agar medium.

[0067] Example 1

[0068] A method for regulating soilless low-temperature vernalization and arrow extraction of Cymbidium, the specific steps are as follows:

[0069] (1) On September 10, choose Cymbidium variety 'Huakai Fugui' (as shown in Figure 1 ) with ungerminated flower buds, remove the pot and soil, prune the rotten roots, rinse the root system with tap water, and dry the root system surface moisture in the seedbed (as shown in Figure 2 ), divide the Cymbidium plants with 5-6 seedlings and flower buds (as shown in Figure 3 ), rinse the root system of potted Cymbidium with flower buds after September, dry it, and divide the plants with flower buds, ready for use;

[0070] (2) the plant with buds is put into a plastic bag containing agar medium (as shown in Figure 4 ), the agar is squeezed to make the medium evenly stick to the roots of the plant, and the plastic bag is tied at the pseudobulb of the plant with an elastic band (as shown in Figure 5 );

[0071] (3) the plant obtained in step (2) is wrapped with an aeration column packaging bag;

[0072] (4) the wrapped plant is stored in a refrigerator at 3-5°C for 45 days (as shown in Figure 6 ), and the plant is completed springing;

[0073] (5) the leaves of the plant completed springing are sprayed with gibberellin GA3 at a concentration of 60 mg / L, and after the spraying is completed, the plant is wrapped with an aeration column packaging bag and then transferred to an environment with a day temperature of 23-27°C and a night temperature of 13-17°C for 17 days (as shown in Figure 7 ), and the plant is completed arrow-pulling, i.e. the springing and arrow-pulling processes of the Cymbidium are completed, and the plant is transplanted or sold according to needs.

[0074] The above only describes the preferred embodiments of the present application, and it should be noted that those skilled in the art can make several improvements and refinements without departing from the principles of the present application, and these improvements and refinements should also be considered as the protection scope of the present application.

Claims

1. A method for regulating soilless low temperature vernalization and arrow extraction of Cymbidium, characterized in that, The method comprises the following steps: (1) washing and drying the roots of Cymbidium, and separating the plants with flower buds for standby; (2) placing the roots of the plants with flower buds into a container containing agar medium; (3) wrapping the plants obtained in step (2) with a buffer product; (4) placing the wrapped plants in an environment with a temperature of 3-5°C for 30-45 days to complete vernalization of the plants; (5) spraying a plant growth regulator on the leaves of the plants after vernalization, wrapping the plants with a buffer product, and then placing the plants in an environment with a day temperature of 23-27°C and a night temperature of 13-17°C for 12-17 days to complete arrow extraction, thereby completing the vernalization and arrow extraction of the Cymbidium.

2. The method for regulating soilless low temperature vernalization and arrow extraction of Cymbidium according to claim 1, wherein, The Cymbidium in step (1) is a potted Cymbidium with flower buds.

3. The method for regulating soilless low temperature vernalization and arrow extraction of Cymbidium according to claim 1, wherein, The agar medium in step (2) is not sterilized at high temperature, and a certain concentration of antibiotics and bactericides are added to the agar medium.

4. The method for regulating soilless low temperature vernalization and arrow extraction of Cymbidium according to claim 1, wherein, The agar medium in step (2) comprises the following components: Medium + 4 million units of penicillin, 98% hymexazol, sodium benzoate, sucrose, agar powder, and activated carbon, wherein the concentration of penicillin is 0.2-0.5 g / L, the concentration of 98% hymexazol is 0.2-0.5 g / L, the concentration of sodium benzoate is 0.05-0.1 g / L, the concentration of sucrose is 15-25 g / L, the concentration of agar powder is 3.0-4.0 g / L, the concentration of activated carbon is 1 g / L, and the pH value is 5.1-5.

4.

5. The method for regulating soilless low temperature vernalization and arrow extraction of Cymbidium according to claim 4, wherein, The preparation method of the agar medium in step (2) comprises the following steps: weighing the medium according to the formula and dissolving it in tap water; adding sucrose and activated carbon in sequence, stirring uniformly, adjusting the pH value to 5.1-5.4, adding agar powder, and stopping heating after stirring and heating to 100°C; when the temperature of the medium falls to 50-45°C, adding penicillin, hymexazol, and sodium benzoate in sequence according to the formula, and stirring uniformly to obtain the agar medium.

6. The method for regulating soilless low temperature vernalization and arrow extraction of Cymbidium according to claim 1, wherein, After placing the roots of the plants with flower buds into the container containing the agar medium in step (2), the plants are tied at the pseudobulb.

7. The method for regulating soilless low temperature vernalization and arrow extraction of Cymbidium according to claim 1, wherein, The buffer product in step (3) is an air-filled packaging bag.

8. The method for regulating soilless low temperature vernalization and arrow extraction of Cymbidium according to claim 1, wherein, The spraying concentration of the plant growth regulator in step (5) is 60-90 mg / L.

9. The method for regulating soilless low temperature vernalization and arrow extraction of Cymbidium according to claim 1, wherein, The plant growth regulator in step (5) comprises gibberellin.

10. The method for regulating soilless low temperature vernalization and arrow extraction of Cymbidium according to claim 1, wherein, The spraying site of the plant growth regulator is the front and back of the plants.

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