Anti-oxidation composition containing celery seed essential oil as well as preparation method and application of anti-oxidation composition

Abstract

The invention relates to an antioxidant composition containing celery seed essential oil as well as a preparation method and application of the antioxidant composition. The antioxidant composition contains celery seed essential oil, vitamin C and vitamin E. The preparation method comprises the following steps: extracting celery seed essential oil by adopting a deep-eutectic solvent, slowly dropwise adding the celery seed essential oil into a main system consisting of vitamin C and vitamin E, stirring to form a transparent and uniform solution, and adjusting the pH value to 3.0. The antioxidant composition can effectively enhance the activity of SOD, has good oxidation resistance, and can be widely applied to the field of cosmetics.

Description

TECHNICAL FIELD

[0001] The present application belongs to the technical field of cosmetics, and relates to an antioxidant composition, in particular to an antioxidant composition containing celery seed essential oil and a preparation method and application thereof. BACKGROUND

[0002] Skin is the first barrier of the human body, and directly reflects the aging condition of the body. When the skin is exposed to ultraviolet rays, environmental pollution, blue light, stress and other external factors, a large number of highly active and unstable molecules, mainly active oxygen, are induced in the body, which damage cells and lead to skin aging, pigmentation and inflammation. Therefore, adding antioxidants to cosmetics can provide a layer of "exogenous protection" for the skin, which can prevent photoaging and environmental aging.

[0003] Common antioxidants include vitamin C and vitamin E. Vitamin C, also known as ascorbic acid, is a benchmark of water-soluble antioxidants, which can directly neutralize free radicals and stimulate collagen synthesis. However, due to its instability and easy oxidation, it is often encapsulated, powder separated or used as a derivative to improve stability and transdermal penetration. Vitamin E is a fat-soluble antioxidant and is the main protector of cell membranes. It is often used in combination with vitamin C to achieve "water-oil dual-phase" antioxidant effect.

[0004] Patent application with publication number CN111657490A provides a dandelion root saccharification liquid, dandelion root fermentation product and its preparation method and product comprising the same, which relates to the technical field of health food. The above-mentioned saccharification liquid comprises dandelion root, barley malt, vitamin C, vitamin E and water. The dandelion root saccharification liquid composed of the above-mentioned raw materials in a specific proportion, and the dandelion root fermentation product further prepared from the dandelion root saccharification liquid, have good antioxidant properties.

[0005] There are also vitamin C and vitamin E used as components of skin care products. Patent application with publication number CN112294720A discloses a composition for preventing skin pigmentation and its application, which comprises 1%-4% glutathione, 1%-3% arbutin, 0.2%-0.6% vitamin C, 0.1%-2% vitamin E, 0.05%-0.1% indomethacin, 0.01%-0.05% 9-carotene, 0.1%-0.6% green tea, 0.2%-0.7% Fructus Mume extract, 0.2%-0.9% Cinnamomum cassia extract, 2%-12% liquorice oil, 1%-6% extract, and the balance is water. The composition still has the effect of inhibiting the formation of melanin under sunlight, and can fully prevent skin pigmentation.

[0006] With the gradual improvement of people's awareness and requirements for skin care and anti-aging, more and more efficient SOD activity enhancing products need to be developed. SUMMARY

[0007] The purpose of this invention is to provide an antioxidant composition that effectively enhances SOD activity, possesses good antioxidant properties, and can be widely used in the cosmetics field.

[0008] Another object of the present invention is to provide a method for preparing the above-mentioned antioxidant composition.

[0009] Another object of the present invention is to provide an application of the above-mentioned product.

[0010] The objective of this invention is achieved through the following solution.

[0011] In a first aspect, the present invention provides an antioxidant composition containing celery seed oil, wherein the antioxidant composition contains celery seed oil, vitamin C, and vitamin E; the pH value of the antioxidant composition is 2.8-3.5; wherein, Celery seed oil accounts for 0.3%-10.0% of the antioxidant composition by mass volume. Vitamin C accounts for 2.0%-15.0% of the antioxidant composition by mass volume. Vitamin E accounts for 0.5%-5.0% of the antioxidant composition by mass volume.

[0012] This invention screened dozens of raw materials and found that celery seed oil, when combined with vitamin C and vitamin E, can effectively enhance SOD activity. Celery seed oil is a volatile aromatic compound extracted by distillation. It has a warm, spicy, slightly musky, and sweet aroma, and its color ranges from colorless to pale yellow. In traditional medicine, celery seeds have been used to treat arthritis, high blood pressure, and indigestion. Modern technology has widely applied it in aromatherapy, high-end perfumes, food flavorings, and functional cosmetics and health products.

[0013] In a preferred embodiment of the present invention, the vitamin C is prepared by weighing 5g of L-ascorbic acid powder, adding it to 100mL of dipropylene glycol, and stirring until a transparent solution is obtained.

[0014] Preferably, the extraction method of celery seed essential oil is as follows: after mixing crushed celery seed powder and eutectic solvent in a certain mass ratio to ensure thorough mixing, reacting at a certain temperature for at least half an hour, more preferably 60 minutes, the volatile oil obtained by steam distillation is collected and dried to obtain celery seed essential oil.

[0015] Thorough mixing means that the celery seed powder and the eutectic solvent have been mixed and reacted as much as possible, for example, reacting in a water bath at 50°C-70°C for at least half an hour, preferably in a water bath at 60°C-65°C for at least one hour, so as to ensure thorough mixing.

[0016] Deep eutectic solvents (DESs) are multicomponent liquids formed by mixing a hydrogen bond donor (e.g. urea, carboxylic acid) with a hydrogen bond acceptor (e.g. quaternary ammonium salt) in a specific molar ratio. The melting point of DESs is significantly lower than that of the individual components due to the hydrogen bonding between the components. DESs have similar physicochemical properties to ionic liquids, including low vapor pressure, high viscosity, and tunable solubility. DESs can be classified into four categories based on the components: quaternary ammonium salt + metal chloride, quaternary ammonium salt + hydrogen bond donor, etc. In addition, natural deep eutectic solvents (NADES) and therapeutic deep eutectic solvents (THEDES) are also included.

[0017] More preferably, the extraction method of the celery seed essential oil is as follows: The dried celery seeds are crushed and passed through an 80-mesh sieve to obtain celery seed powder; Choline chloride and 1,3-propanediol are added to a round-bottom flask in a 1:2 ratio, and heated and stirred in a water bath at 60°C for at least half an hour to form a uniform and clear deep eutectic solvent; The celery seed powder is mixed with the deep eutectic solvent in a mass ratio of 1:4, and stirred in a water bath at 60°C for at least 8 minutes to obtain a mixture; 200 ml of deionized water is added to the mixture, and water vapor distillation is performed for 1 hour. The volatile oil obtained by distillation is collected and dried.

[0018] In a preferred embodiment of the present application, choline chloride and 1,3-propanediol are heated and stirred in a water bath at 60°C for at least half an hour to form a uniform and clear deep eutectic solvent.

[0019] Preferably, the preparation method of the antioxidant composition is as follows: celery seed essential oil and PEG-40 hydrogenated castor oil are mixed in a certain ratio to obtain a uniform solution. The mixing ratio is 1: (2-5) by mass of celery seed essential oil and deep eutectic solvent. Then the solution is slowly added dropwise to the main system, and continuous rapid stirring is performed to form a transparent and uniform solution. The pH value is adjusted to 3.0.

[0020] The main system is a mixture containing vitamin C and vitamin E.

[0021] Preferably, the pH value of the antioxidant composition is 3.0-3.2.

[0022] Preferably, the mass / volume ratio of celery seed essential oil in the antioxidant composition is 0.5%-1.5%.

[0023] Preferably, the mass / volume ratio of vitamin C in the antioxidant composition is 5.0%-10.0%.

[0024] Preferably, the mass / volume ratio of vitamin E in the antioxidant composition is 1.0%-2.0%.

[0025] In a second aspect, the present application provides a method for preparing an antioxidant composition containing celery seed essential oil, which comprises the following steps: (1) Pre-solubilizing Vitamin C: stirring L-ascorbic acid powder until completely dissolved to obtain a transparent solution of L-ascorbic acid with a mass / volume ratio of 2.0%-15.0%; (2) adding Vitamin E to the transparent solution obtained in step (1), wherein the mass / volume ratio of Vitamin E is 0.5%-5.0%, and stirring uniformly; (3) Pre-solubilizing celery seed essential oil: mixing celery seed essential oil and a deep eutectic solvent, wherein the mass ratio of celery seed essential oil to deep eutectic solvent is 1:(2-5), and stirring until completely clear and transparent to form a celery seed essential oil solubilizing solution; (4) adding the celery seed essential oil solubilizing solution prepared in step (3) dropwise to the product obtained in step (2), and continuously stirring to form a transparent and uniform solution; (5) detecting the pH value and adjusting it to 2.8-3.5.

[0026] In the present application, the method for preparing an antioxidant composition containing celery seed essential oil does not necessarily follow the order of steps (1)-(5), but can follow the logic of conventional techniques in the art. For example, the step of pre-solubilizing celery seed essential oil can be completed before step (4), or before or after steps (1) or (2).

[0027] Preferably, in step (1), 2.0g-15.0g of L-ascorbic acid powder is added per 100mL of dipropylene glycol, and the mixture is stirred uniformly to obtain a transparent solution. More preferably, 5.0g-10.0g of L-ascorbic acid powder is added per 100mL of dipropylene glycol, and the mixture is stirred uniformly to obtain a transparent solution.

[0028] Preferably, in step (2), 0.5g-5.0g of Vitamin E is added to the transparent solution containing Vitamin C obtained in step (1), and the mixture is stirred uniformly. More preferably, 1.0g-2.0g of Vitamin E is added to the transparent solution containing Vitamin C obtained in step (1), and the mixture is stirred uniformly.

[0029] Preferably, in step (3), 0.3g-10.0g of celery seed essential oil and 0.9g-30.0g of a deep eutectic solvent are weighed and mixed, and the mixture is stirred until completely clear and transparent to form a celery seed essential oil solubilizing solution. More preferably, 0.5g-1.5g of celery seed essential oil and 1.5g-4.5g of a deep eutectic solvent are weighed and mixed, and the mixture is stirred until completely clear and transparent to form a celery seed essential oil solubilizing solution.

[0030] In a preferred embodiment of the present invention, the preparation method includes the following steps: (1) Pre-dissolved vitamin C: Weigh 5g of L-ascorbic acid powder, add it to 100mL of dipropylene glycol, and stir at medium speed with a magnetic stirrer until completely dissolved to obtain a transparent solution.

[0031] (2) Add 1.0g of vitamin E to the vitamin C solution and stir well.

[0032] (3) Pre-dissolved celery seed oil: Weigh a certain amount of celery seed oil and PEG-40 hydrogenated castor oil, mix them in a small beaker, and gently stir with a glass rod until completely clear and transparent to form an "essence oil enrichment solution" for later use.

[0033] (4) Slowly and dropwise add the premixed “celery seed oil solution” from step (3) into the main system and stir continuously and rapidly to form a transparent and uniform solution.

[0034] (5) Use a pH meter to measure the pH value, and use a very small amount of acid or alkali to finally and accurately adjust it to 3.0.

[0035] Thirdly, the present invention provides the use of an antioxidant composition containing celery seed oil in the cosmetic field.

[0036] The antioxidant composition containing celery seed essential oil of the present invention can be added to skin care products and cosmetics as an antioxidant ingredient, and can be made into antioxidant products, free radical scavenging products, anti-aging products, etc.

[0037] Tests show that the oxidative composition of the present invention effectively enhances SOD activity, achieving a high SOD activity value of over 12. The oxidative composition of the present invention possesses excellent antioxidant properties and can be widely used in the cosmetics field, including enhancing the fight against free radicals and delaying skin aging. Detailed Implementation

[0038] The technical solution will be clearly and completely described below through embodiments of this application. Obviously, the described embodiments are only some preferred embodiments of this application, and not all embodiments. Based on the embodiments of this application, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of this application.

[0039] Example 1 Celery seed extraction process (1) The dried celery seeds are crushed by a pulverizer and passed through an 80-mesh sieve to obtain celery seed powder, which is then transferred to a desiccator for storage.

[0040] (2) Choline chloride, 1,3-propanediol were added into a round bottom flask in a ratio of 1:2, and mixed and heated in a constant temperature water bath at 60°C for 60 min to form a homogeneous and clear eutectic solvent.

[0041] (3) Celery seed powder was mixed with the eutectic solvent in a mass ratio of 1:4 and added into a round bottom flask, which was stirred in a constant temperature water bath at 60°C for 10 min.

[0042] (4) The mixture of step (3) was added into 200 ml of deionized water, and water vapor distillation was performed in a distillation apparatus for 1 h. The volatile oil obtained by distillation was collected and dried with anhydrous sodium sulfate before being stored for use.

[0043] Example 2 Preparation of an antioxidant composition (1) Pre-solubilize vitamin C: 5 g of L-ascorbic acid powder was weighed and added into 100 mL of dipropylene glycol, which was stirred at a medium speed by a magnetic stirrer until completely dissolved to obtain a transparent solution; (2) 1.0 g of vitamin E was added into the vitamin C solution and stirred uniformly to form a main system; (3) Pre-solubilize celery seed essential oil: 1.5 g of celery seed essential oil and 4.5 g of PEG-40 hydrogenated castor oil were weighed and mixed in a small beaker, which was gently stirred with a glass rod until completely clear and transparent to form an "essential oil solubilizing solution" for standby; (4) The "celery seed essential oil solubilizing solution" prepared in step 3 was slowly and dropwise added into the main system, and a transparent and uniform solution was formed by continuous and rapid stirring; (5) The pH value was detected by a pH meter, and a trace amount of acid or base was used to finally adjust it to 3.0.

[0044] Example 3 Preparation of an antioxidant composition (1) Pre-solubilize vitamin C: 5 g of L-ascorbic acid powder was weighed and added into 100 mL of dipropylene glycol, which was stirred at a medium speed by a magnetic stirrer until completely dissolved to obtain a transparent solution; (2) 1.0 g of vitamin E was added into the vitamin C solution and stirred uniformly to form a main system; (3) Pre-solubilize celery seed essential oil: 1.5 g of celery seed essential oil and 4.5 g of PEG-40 hydrogenated castor oil were weighed and mixed in a small beaker, which was gently stirred with a glass rod until completely clear and transparent to form an "essential oil solubilizing solution" for standby; (4) The "celery seed essential oil solubilizing solution" prepared in step 3 was slowly and dropwise added into the main system, and a transparent and uniform solution was formed by continuous and rapid stirring;

[0045] (5) The pH value is detected by a pH meter, and a trace amount of acid or base is used to finally adjust it to 3.0.

[0046] Example 4 Preparation of the antioxidant composition (1) Pre-solubilize vitamin C: weigh 5 g of L-ascorbic acid powder, add it to 100 mL of dipropylene glycol, and stir it at medium speed with a magnetic stirrer until it is completely dissolved to obtain a transparent solution; (2) Add 1.0 g of vitamin E to the vitamin C solution and stir it evenly to form the main system; (3) Pre-solubilize celery seed essential oil: weigh 0.5 g of celery seed essential oil and 1.5 g of PEG-40 hydrogenated castor oil, mix them in a small beaker, and stir them gently with a glass rod until they are completely clear and transparent to form an "essential oil solubilizing solution" for standby; (4) Slowly and dropwise add the pre-mixed "celery seed essential oil solubilizing solution" of step 3 to the main system, continue to stir quickly, and form a transparent and uniform solution.

[0047] (5) The pH value is detected by a pH meter, and a trace amount of acid or base is used to finally adjust it to 3.0.

[0048] Performance test method After the sample of one bottle of serum albumin freeze-dried powder is completely mixed with one bottle of solution, then each component is added according to the following table, wherein A test group: sample group, positive control group, B test blank group, C negative control group, D negative control blank group. At the end of the test, take each reaction solution into the corresponding 96-well plate, 3 parallel for each group, and read at 450 nm with an enzyme marker after the reaction is completed .

[0049] In the formula: A—A (enzyme working solution + sample / positive control + substrate application liquid) OD value of the hole test sample solution; B—B (enzyme dilution solution + sample / positive control + substrate application liquid) OD value of the hole test sample solution; C—C (enzyme working solution + pure water + substrate application liquid) OD value of the hole test sample solution; D—D (enzyme dilution solution + pure water + substrate application liquid) OD value of the hole test sample solution.

[0050] Definition: In this reaction system, the amount of enzyme corresponding to 50% SOD inhibition is one SOD activity unit (U).

[0051] Formula for calculating the activity of SOD: SOD activity (U / mL) = SOD inhibition rate ÷ 50% × dilution multiple of reaction system ÷ dilution multiple of sample before test.

[0052] In the formula, the dilution multiple of the reaction system is 0.24 mL / 0.02 mL.

[0053] Table 2: SOD activity test results of the antioxidant compositions obtained in Examples 2, 3 and 4 As can be seen from the experimental results in Table 2, the SOD activity values of the antioxidant compositions obtained in Examples 2, 3 and 4 are all high, which indicates that the antioxidant compositions of the present application can effectively enhance the SOD activity and have good antioxidant property. In particular, the SOD activity of Example 2 is the highest.

[0054] In the description of the present application, the term "comprising", "containing" or any other variant thereof is intended to cover non-exclusive inclusion, so that the process, method, article or equipment including a series of elements not only includes those elements, but also includes other elements not explicitly listed or inherent to such process, method, article or equipment. Without more limitations, the element defined by the statement "comprising a reference structure" does not exclude the presence of other identical elements in the process, method, article or equipment including the element. It should be noted that in this paper, the relationship terms such as "first", "second" and the like are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between the entities or operations.

[0055] The above-described embodiments are only specific embodiments of the present application, but the protection scope of the present application is not limited thereto. Any skilled person in the art can conceive of changes or replacements within the technical scope disclosed in the present application without creative labor, which should be covered within the protection scope of the present application. Therefore, the protection scope of the present application should be subject to the protection scope of the claims in the present application.

Claims

1. An antioxidant composition comprising a celery seed essential oil, characterized in that, The antioxidant composition contains celery seed essential oil, vitamin C and vitamin E; the pH value of the antioxidant composition is 2.8-3.5; wherein, The mass / volume ratio of celery seed essential oil in the antioxidant composition is 0.3%-10.0%; The mass / volume ratio of vitamin C in the antioxidant composition is 2.0%-15.0%; The mass / volume ratio of vitamin E in the antioxidant composition is 0.5%-5.0%.

2. The antioxidant composition containing the oil of the seeds of the Apiaceae family according to claim 1, characterized in that, The extraction method of celery seed essential oil is as follows: the crushed celery seed powder and the eutectic solvent are mixed, then fully reacted, the volatile oil obtained by water vapor distillation is collected, and then dried to obtain the celery seed essential oil.

3. The antioxidant composition containing the oil of the seeds of the Apiaceae family according to claim 1, characterized in that, The preparation method of the antioxidant composition is as follows: the celery seed essential oil and PEG-40 hydrogenated castor oil are mixed to obtain a uniform solution, then the solution is slowly added dropwise into the main system, continuous rapid stirring is performed to form a transparent and uniform solution, and the pH value is adjusted to 2.8-3.5; The main system contains a mixture of vitamin C and vitamin E.

4. The antioxidant composition comprising the oil of the seeds of Apium graveolens according to claim 1, characterized in that, The pH value of the antioxidant composition is 3.0-3.2; The mass / volume ratio of celery seed essential oil in the antioxidant composition is 0.5%-1.5%; The mass / volume ratio of vitamin C in the antioxidant composition is 5.0%-10.0%; The mass / volume ratio of vitamin E in the antioxidant composition is 1.0%-2.0%.

5. A process for the preparation of the antioxidant composition containing parsley seed essential oil according to any one of claims 1 to 4, characterized in that, The method comprises the following steps: (1) Pre-solubilize vitamin C: take L-ascorbic acid powder and stir until completely dissolved to obtain a transparent solution of L-ascorbic acid with a mass / volume ratio of 2.0%-15.0%; (2) Add vitamin E to the transparent solution obtained in step (1), wherein the mass / volume ratio of vitamin E is 0.5%-5.0%, and stir uniformly; (3) Pre-solubilize celery seed essential oil: mix celery seed essential oil and PEG-40 hydrogenated castor oil, the mass ratio of celery seed essential oil to PEG-40 hydrogenated castor oil is 1: (2-5), stir until completely clear and transparent to form a celery seed essential oil solubilized solution, and reserve; (4) Add the celery seed essential oil solubilized solution prepared in step (3) dropwise into the product obtained in step (2), continuously stir to form a transparent and uniform solution; (5) Detect the pH value and adjust it to 2.8-3.

5.

6. The preparation method according to claim 5, wherein, In step (1), 2.0g-15.0g of L-ascorbic acid powder is added into 100mL of dipropylene glycol, and stirred uniformly to obtain a transparent solution; In step (2), 0.5g-5.0g of vitamin E is added into the transparent solution containing vitamin C obtained in step (1), and stirred uniformly; or, In step (3), 0.3g-10.0g of celery seed essential oil and 0.9g-30.0g of eutectic solvent are weighed and mixed, and stirred until completely clear and transparent to form a celery seed essential oil solubilized solution.

7. The preparation method according to claim 5, wherein, In step (1), 5.0g-10.0g of L-ascorbic acid powder is added into 100mL of dipropylene glycol, and stirred uniformly to obtain a transparent solution; In step (2), 1.0-2.0 g of vitamin E is added to the transparent solution containing vitamin C obtained in step (1) and stirred until uniform; In step (3), 0.5-1.5 g of celery seed essential oil and 1.5-4.5 g of pre-solubilized celery seed essential oil are weighed and mixed and stirred until completely clear and transparent to form a celery seed essential oil solubilized solution, which is ready for use; or, In step (5), the pH value is adjusted to 3.0-3.

2.

8. The preparation method according to claim 5, characterized in that, The eutectic solvent is obtained by mixing choline chloride and 1,3-propanediol and heating and stirring for at least half an hour to form a uniform clear liquid.

9. The preparation method according to claim 5, characterized in that, The extraction method of celery seed essential oil is as follows: The dried celery seeds are crushed and passed through an 80-mesh sieve to obtain celery seed powder; Choline chloride and 1,3-propanediol are added to a round-bottom flask in a ratio of 1:2 and mixed and heated and stirred in a water bath at 60°C for at least half an hour to form a uniform clear eutectic solvent; The celery seed powder is mixed with the eutectic solvent in a ratio of 1:4 and stirred in a water bath at 60°C for at least 8 min to obtain a mixture; 200 ml of deionized water is added to the mixture and water vapor is distilled for 1 h, and the volatile oil obtained by distillation is collected and dried.

10. Use of the antioxidant composition containing celery seed essential oil according to any one of claims 1-4 in the field of cosmetics.

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