Processing method based on drug effect substance of codonopsis pilosula and activity of drug effect substance

Abstract

The invention discloses a processing method based on codonopsis pilosula pharmacodynamic substances and activity thereof, and relates to the field of traditional Chinese medicine processing, and the processing method comprises the following steps: S1, taking a codonopsis pilosula medicinal material, and pretreating to obtain codonopsis pilosula decoction pieces; s2, the codonopsis pilosula decoction pieces are processed, the processing is rice frying, and the processing parameters are as follows: rice is selected as an auxiliary material according to the mass ratio of codonopsis pilosula to the auxiliary material being 100: 20, the rice is put into a frying pan, the rice is heated to smoke with medium fire, the codonopsis pilosula decoction pieces are put into the frying pan, and stir-frying is performed for 8-12 min under the conditions of 150-170 DEG C and 2.2-2.6 kW; according to the processing method, the content of lobetyolin, the alcohol-soluble extract, the appearance property and the moisture in the codonopsis pilosula are used as detection indexes, key parameters of the codonopsis pilosula processing technology are accurately quantified and optimized, quality fluctuation caused by manual operation differences is effectively eliminated, and the quality of the codonopsis pilosula is improved. And the advantages in the aspects of pharmacodynamic substances and biological activity of the codonopsis pilosula are reserved and stimulated to the maximum extent.

Description

TECHNICAL FIELD

[0001] The present application relates to the processing technology of traditional Chinese medicine, in particular to a processing method based on the medicinal material and activity of radix codonopsis. BACKGROUND

[0002] Radix codonopsis is the dried root of the plant Codonopsis pilosula, which is a commonly used tonic traditional Chinese medicinal material. It has the functions of invigorating the spleen and lung and nourishing blood and generating fluid. Processing is an important processing link before the clinical application of traditional Chinese medicine, aiming to enhance the efficacy, change the drug nature or reduce the side effects. As one of the mainstream processing methods of radix codonopsis, rice frying has been recorded in ancient books. For example, rice-fried radix codonopsis can be used to treat spleen deficiency and cold diarrhea, as mentioned in the book Shilun of Qing Dynasty. Modern research has also shown that rice-fried radix codonopsis has a clear and sweet smell, and its and stomach, spleen and diarrhea-stopping effects are enhanced.

[0003] However, the current processing technology of rice-fried radix codonopsis in the Chinese Pharmacopoeia (2020 edition) is only described qualitatively (such as "until the radix codonopsis turns yellow"), and lacks precise and controllable process parameters such as frying temperature, time, fire power and the amount of auxiliary materials. This traditional processing method relying on the experience of operators directly leads to significant fluctuations in the key quality indicators of rice-fried radix codonopsis decoction pieces prepared by different batches and different production units, such as radix codonopsis glycosides, alcohol-soluble extract, appearance and moisture content. The quality uniformity and stability are difficult to guarantee, which seriously restricts the modernization and standardization of traditional Chinese medicine decoction pieces. SUMMARY

[0004] The purpose of the present application is to provide a processing method based on the medicinal material and activity of radix codonopsis, in order to solve the problem of unclear processing parameters in the prior art, which leads to unstable and poor uniformity of the final decoction pieces.

[0005] In order to achieve the above-mentioned purpose, the present application provides the following technical scheme: a processing method based on the medicinal material and activity of radix codonopsis, comprising:

[0006] S1, taking radix codonopsis medicinal materials, and obtaining radix codonopsis decoction pieces after pretreatment;

[0007] S2, processing the radix codonopsis decoction pieces, the processing parameter is rice frying, and the processing parameter is: selecting rice as auxiliary material according to the quality ratio of radix codonopsis to auxiliary material 100:20, putting rice into a frying pan, heating to smoke under medium fire, and then putting radix codonopsis decoction pieces, frying for 8-12 min under the condition of 150-170 DEG C and 2.2-2.6 kW, and taking out when the radix codonopsis turns yellow and has a clear and sweet smell;

[0008] S3, screening the auxiliary materials of the fried radix codonopsis, and obtaining radix codonopsis processing products after cooling to room temperature.

[0009] Further, the processing treatment is also soil frying, and the processing parameters are as follows: 25-35% of hearth soil by weight of the decoction piece of radix codonopsis is placed in a pan and fried until flexible, the decoction piece is put in, and the decoction piece is taken out when the surface of the decoction piece is fried to deep yellow and the smell of burnt smell is emitted.

[0010] Further, the processing parameters of the rice frying are as follows: the frying temperature is 160℃, the frying time is 10 min, the amount of rice is 20% of the weight of the decoction piece of radix codonopsis, and the frying power is 2.4kW.

[0011] Further, the radix codonopsis medicinal material is dry root of radix codonopsis or radix codonopsis in Lu.

[0012] Further, the radix codonopsis medicinal material is radix codonopsis in white strip.

[0013] Further, the preprocessing includes washing and cutting the radix codonopsis medicinal material, and drying until the hand is not sticky to obtain the decoction piece of radix codonopsis.

[0014] Further, the content of codonopsis glycoside in the processed product of radix codonopsis is ≥0.013%, the ABTS free radical scavenging rate is ≥78% under the concentration of 100ug / mL, and the DPPH free radical scavenging rate is ≥76%.

[0015] Compared with the prior art, the processing method based on the medicinal material of radix codonopsis and the activity thereof provided by the present application accurately quantifies and optimizes the key parameters of the processing technology of radix codonopsis by taking the content of codonopsis glycoside, alcohol-soluble extract, appearance and moisture in radix codonopsis as detection indexes, effectively eliminates the quality fluctuation caused by manual operation difference, makes the rice-fried radix codonopsis decoction piece produced in different batches have high consistency and repeatability in appearance, moisture content, internal components and the like, and maximizes the advantages of retaining and stimulating the medicinal material and biological activity of radix codonopsis. BRIEF DESCRIPTION OF DRAWINGS

[0016] In order to more clearly illustrate the technical solutions in the embodiments of the present application or the prior art, the drawings needed in the embodiments will be briefly introduced below. Obviously, the drawings in the following description are only some embodiments described in the present application, and other drawings can also be obtained by those skilled in the art according to these drawings.

[0017] Figure 1 The influence curve diagram of different frying temperatures on radix codonopsis provided by the first embodiment of the present application;

[0018] Figure 2 The influence curve diagram of different frying times on radix codonopsis provided by the first embodiment of the present application;

[0019] Figure 3 The influence curve diagram of different frying powers on radix codonopsis provided by the first embodiment of the present application;

[0020] Figure 4 The ABTS free radical scavenging activity determination curve diagram of different party ginseng saponins provided for the second embodiment of the application is shown in the following figure:

[0021] Figure 5 The DPPH free radical scavenging activity determination curve diagram of different party ginseng saponins provided for the second embodiment of the application is shown in the following figure:

[0022] Figure 6 The determination curve diagram of the iron reduction antioxidant capacity of different party ginseng saponins provided for the second embodiment of the application is shown in the following figure. DETAILED DESCRIPTION

[0023] In order for those skilled in the art to better understand the technical solutions of the present application, the present application will be further described in detail below in conjunction with the accompanying drawings.

[0024] As shown in the accompanying Figure 1 to the accompanying Figure 6 , as shown in the following figure:

[0025] Embodiment I:

[0026] The present application provides a processing method based on the medicinal effect substance of party ginseng and its activity, comprising:

[0027] S1, taking party ginseng medicinal materials, and obtaining party ginseng decoction pieces after pretreatment;

[0028] S2, processing 500g of party ginseng decoction pieces, the processing is rice frying, and the processing parameters are: selecting rice as auxiliary material according to the mass ratio of party ginseng to auxiliary material 100:20, putting the rice into a frying pan, heating to smoke under medium fire, and then putting in party ginseng decoction pieces, and frying for 10min under the condition of 160℃, 2.4kW, and taking out when the party ginseng is slightly yellow and has a pleasant aroma;

[0029] S3, screening the auxiliary material of the fried party ginseng, and obtaining party ginseng processed products after cooling to room temperature.

[0030] Specifically, the party ginseng raw material is white strip party ginseng.

[0031] Specifically, the pretreatment includes washing the party ginseng medicinal materials, cutting them, and drying them until they are not sticky to the hand to obtain party ginseng decoction pieces.

[0032] Trace elements Mn are added in the processing process.

[0033] Specifically, the content of party ginseng yin is ≥0.013% in the party ginseng processed products, the ABTS free radical scavenging rate is ≥78% under the concentration of 100ug / mL, and the DPPH free radical scavenging rate is ≥76%.

[0034] Performance test: In this embodiment, the experimental results are standardized by using a multi-index comprehensive scoring method. The processing method is Yi / Ymax. According to the importance of each index to the fried Codonopsis, the comprehensive score Y = content of codonopsis glycoside × 30% + content of alcohol-soluble extract × 30% + appearance score × 20% + moisture score × 20%;

[0035] The above obtained Codonopsis processed products were sequentially detected for content of codonopsis glycoside component, alcohol-soluble extract, appearance, and moisture content.

[0036] Among them:

[0037] Content of codonopsis glycoside component:

[0038] Column: Caprisil C18 5µm 100Å 250mm×4.6mm

[0039] Mobile phase: 0.1% formic acid aqueous solution (A) - acetonitrile (B);

[0040] Flow rate: 0.4mL / min;

[0041] Column temperature: 30℃;

[0042] Detection wavelength: 257nm;

[0043] The peak area of codonopsis glycoside was recorded by computer,

[0044] The peak area of codonopsis glycoside in the test solution was compared with that of the control solution. The accurate content of codonopsis glycoside in the codonopsis sample was calculated by standard curve or external standard method.

[0045] Appearance: Appearance evaluation is one of the important quality evaluation standards for traditional Chinese medicine decoction pieces. According to the description of the appearance of fried Codonopsis in the 2020 edition of Chinese Pharmacopoeia, the scoring standard prepared in this embodiment is as follows: 4 points for brownish yellow, 7 points for yellow, and 10 points for light yellow; 4 points for burnt smell, no smell, 7 points for smell, and 10 points for slight smell.

[0046] Determination of alcohol-soluble extract: determined according to the hot extraction method under alcohol-soluble extract determination method (general rule 2201 of Chinese Pharmacopoeia), 2 parallel samples.

[0047] Moisture determination: After the sample is crushed through No. 3 sieve, its moisture is determined by drying method according to the 2020 edition of Chinese Pharmacopoeia. The 2020 edition of Chinese Pharmacopoeia stipulates that the moisture of fried Dangshen is not more than 10.0%. Since the moisture content of the decoction pieces is too high or too low, it is not conducive to the storage of Dangshen decoction pieces. Therefore, the moisture is scored. In order to facilitate comprehensive scoring, the scoring standard is as follows: the moisture is in the range of 4.0% to 5.0%, and the score is 100 points; the moisture is in the range of 3.0% to 4.0% and 5.0% to 6.0%, and the score is 80 points; the moisture is in the range of 2.0% to 3.0% and 6.0% to 7.0%, and the score is 60 points; the moisture is less than 2.0% or more than 7.0%, and the score is 50 points, and each group has three parallel samples.

[0048] The Dangshen prepared under the above processing parameters and another 5 Dangshen prepared by frying at different temperatures (100, 120, 140, 160, 180, 200°C) were determined and then scored comprehensively.

[0049] Results: When the frying temperature is 160°C, the comprehensive score is the highest.

[0050] The Dangshen prepared under the above processing parameters and another 5 Dangshen prepared by frying at different times (5, 8, 10, 12, 15, 20 min) were determined and then scored comprehensively.

[0051] Results: When the frying time is 10 min, the comprehensive score is the highest.

[0052] The Dangshen prepared under the above processing parameters and another 4 Dangshen prepared by frying at different powers (0.6 kW, 1.2 kW, 1.8 kW, 3 kW) were determined and then scored comprehensively.

[0053] Results: When the power is 2.4 kW, the comprehensive score is the highest.

[0054] In summary: the frying temperature is 160°C, the frying time is 10 min, and the power is 2.4 kW, which is the best processing technology. Under this processing condition, the indicators of Dangshen are all the best.

[0055] Example Two

[0056] This example is basically the same as the previous example, except that wild Dangshen or Lu Dangshen is used. Experimental determination shows that the ABTS free radical scavenging ability, DPPH free radical scavenging ability, and iron reduction antioxidant capacity of Dangshen prepared in this example are weaker than those of Dangshen prepared in Example One.

[0057] Experimental determination is as follows:

[0058] Reagents: 1,1-diphenyl-2-picrylhydrazyl (DPPH, batch number 2404003), 2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS, batch number 061623231020), ferric reducing / antioxidant power assay (FRAP, batch number 070423240222), absolute ethanol, 95% ethanol, pure water.

[0059] Extraction and isolation of ginsenoside components

[0060] The three kinds of powdered Codonopsis pilosula from different origins (wild Codonopsis pilosula, white strip Codonopsis pilosula and Lu Codonopsis pilosula) that had been crushed and passed through a No. 3 sieve were precisely weighed at a material-to-liquid ratio (1:31.35) at 7.98 g, and 250 ml of 69.5% ethanol was measured. The extraction was performed at 75 degrees Celsius for 150 minutes. After the reflux was completed, hot filtration was performed, followed by vacuum distillation. Finally, water bath heating was performed at a temperature of 70 degrees Celsius for 4.5 hours to obtain the ginsenoside components from the three kinds of Codonopsis pilosula from different origins. Finally, the extract was washed out with absolute ethanol, and the volume was adjusted to 20 ml in a brown volumetric flask to obtain the ginsenoside sample solutions from the three kinds of Codonopsis pilosula from different origins.

[0061] Determination of antioxidant activity

[0062] ABTS radical scavenging experiment

[0063] When the ABTS radical scavenging capacity detection was performed, the ABTS radical scavenging capacity of the ginsenosides of Codonopsis pilosula from different origins was determined according to the specific operation of the 2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt kit instructions. Equal volumes (each 10 microliters) of ABTS solution and oxidant solution were uniformly mixed. After being placed in the dark for 16 hours, a dark solution was obtained, which was the ABTS stock solution. At this time, the ginsenoside components of the Codonopsis pilosula to be tested were non-water-soluble components, and the dilution multiple of the ABTS working stock solution was 40 times, that is, 8 ml of 80% ethanol was added to the ABTS stock solution to obtain the ABTS working solution. Then, the three kinds of Codonopsis pilosula ginsenoside sample solutions were diluted to a certain concentration gradient. 10 microliters of the extract of different concentrations was taken with a pipette and added to 200 microliters of the ABTS working solution to measure the radical scavenging activity in the well plate. Ascorbic acid (Vc) was used as the positive control. 10 microliters of distilled water was added to each well of the well plate as the blank control. Then, the computer program was operated, and the enzyme marker was set to measure the wavelength of 734 nm in the enzyme marker that had been turned on and preheated for more than 30 minutes. The absorbance of each was measured at this wavelength. Finally, the ABTS radical scavenging rate of the ginsenoside components of Codonopsis pilosula from different origins was calculated.

[0064] DPPH radical scavenging experiment

[0065] The scavenging ability of DPPH free radicals of saponin components from Codonopsis pilosula from different producing areas was determined according to the specific method of 1,1-diphenyl-2-trinitrobenzene hydrazine kit instructions. An appropriate amount of DPPH reagent was added to 6.08 mL of anhydrous ethanol, and was fully shaken and dissolved to prepare a DPPH primary reagent. A DPPH working solution was prepared in a ratio of DPPH primary reagent: anhydrous ethanol (V:V) = 4:21. 10 mg of vitamin C was added to 1 mL of DPPH extraction solution, fully shaken and dissolved to prepare a 10 mg / ml vitamin C solution for positive control. The sample concentration was diluted into three different gradients according to the operation shown in the kit, and the 10 mg / ml vitamin C solution was prepared into 0.3, 0.25, 0.125, 0.0625, 0.03125, 0.015625 mg / ml vitamin C solution for use. 25 microliters of extraction solution was added to 975 microliters of DPPH working solution and placed in an EP tube as blank sample A (blank); 25 microliters of supernatant was added to 975 microliters of DPPH working solution and placed in an EP tube as determination sample (A determination); 25 microliters of supernatant was added to 975 microliters of anhydrous ethanol and placed in an EP tube as control sample (A control); 25 microliters of vitamin C solution was added to 975 microliters of DPPH working solution and placed in an EP tube as positive control sample A (positive control). After the different samples were prepared, they were placed in a dark place for 30 minutes, and the absorbance was measured at 515 nm. The following calculation formula was used:

[0066] The free radical scavenging rate of positive control was calculated by the following formula:

[0067] DPPH free radical scavenging rate Dvc% = [ (A blank - A positive control) ÷ A blank] × 100%.

[0068] The free radical scavenging rate of sample was calculated by the following formula:

[0069] DPPH free radical scavenging rate D% = {[A blank - (A determination - A control)] ÷ A blank} × 100%.

[0070] Iron reduction / antioxidant capacity (FRAP) determination experiment

[0071] The reducing / antioxidant capacity (FRAP) of saponin components of Codonopsis pilosula from different origins was determined according to the instructions of the ferric ion reducing antioxidant activity assay. 750 microliters of TPTZ diluent and 75 microliters of TPTZ solution were measured with a pipette into a brown volumetric flask, and after being thoroughly shaken and mixed, 75 microliters of detection buffer was obtained, and a total of 900 microliters of FRAP working solution was obtained. The obtained FRAP working solution was placed in a water bath at 37 degrees Celsius for 5 minutes. At the same time, 27.8 mg of FeSO4.7H2O was accurately weighed with a one-hundredth electronic balance, dissolved with distilled water, and made up to 1 ml, at this time the concentration was 100 mM. Take an appropriate amount of 100 mM FeSO4 solution to dilute to 0.15, 0.3, 0.6, 0.9, 1.2 and 1.5 mM. In the 96-well plate, 5 milliliters of distilled water and 180 microliters of FRAP working solution were added to the blank control; 5 microliters of sample solution and 180 microliters of FRAP working solution were added to the sample detection hole; 5 microliters of various concentrations of FeSO4 standard solution and 180 microliters of FRAP working solution were added to the standard control hole.

[0072] At this time, the enzyme marker instrument which has been preheated for more than 30 minutes is opened according to the corresponding operation and the wavelength is set to 593 nm, and the absorbance of different concentrations of Codonopsis pilosula saponin is determined at this wavelength.

[0073] Results:

[0074] ABTS free radical scavenging capacity of saponins from Codonopsis pilosula from different origins:

[0075] ABTS is oxidized to green ABTS+ under the action of a suitable oxidant, and the production of ABTS+ is inhibited in the presence of antioxidants. The absorbance of ABTS+ at 734 nm was measured to determine and calculate the total antioxidant capacity of saponins from three different origins of Codonopsis pilosula. From Figure 4 It can be seen from Table 1 that in the ABTS free radical scavenging experiment, the ABTS free radical scavenging capacity of Bai-Dao Codonopsis pilosula saponin at a concentration of 100 ug / ml was the strongest, and the ABTS free radical scavenging rate could reach 79.5%±0.7071, and the ABTS free radical scavenging rate of wild Codonopsis pilosula saponin at the same concentration was 71%±4.2426 and the ABTS free radical scavenging rate of Lu-Dao saponin at the same concentration was 72%±2.8284, which had significant difference and *P<0.05.

[0076] DPPH free radical scavenging capacity of saponins from Codonopsis pilosula from different origins:

[0077] DPPH free radical is a very stable nitrogen-centered free radical, which is one of the important indicators of the antioxidant capacity of the sample. DPPH free radical has a single electron, and its alcohol solution is purple, with strong absorption at 515 nm. When antioxidants are present, DPPH free radicals are scavenged, and the solution color becomes lighter, and the absorbance at 515 nm decreases. Within a certain range, the change in absorbance is proportional to the degree of free radical scavenging. In this experiment, the degree of absorbance decrease reflects the ability of the sample to scavenge DPPH free radicals. It can be seen from Figure 5 that in the DPPH free radical scavenging experiment, ginsenosides from different producing areas of Codonopsis also exhibit different degrees of antioxidant capacity. The saponin components of Bai Tiao Codonopsis have a strong scavenging effect on DPPH free radicals at a lower concentration, while the saponins of wild Codonopsis and Lu Codonopsis are relatively weak. Bai Tiao Codonopsis has the strongest DPPH free radical scavenging capacity at a concentration of 100 ug / ml, with a scavenging rate of 77% ± 1.4142. At the same concentration, the Lu Codonopsis saponin has a DPPH free radical scavenging rate of 75% ± 1.4142, and the wild Codonopsis saponin has a DPPH free radical scavenging rate of 69% ± 1.4142. The DPPH free radical scavenging capacity of Bai Tiao Codonopsis saponin is significantly different from that of wild Codonopsis saponin, and *p < 0.05.

[0078] Iron-reducing antioxidant capacity (FRAP) of saponins from Codonopsis from different producing areas:

[0079] The principle of FRAP method for determining antioxidant capacity is that under acidic conditions, antioxidants can reduce iron tripyridine triazine to produce blue-colored ferrous tripyridine triazine complex, and then the blue ferrous tripyridine triazine is measured at 593 nm to obtain the total antioxidant capacity of the sample. From Figure 6 it can be seen that in the FRAP determination experiment, the saponins of Codonopsis from different producing areas also have obvious differences in iron-reducing antioxidant capacity. Among them, Bai Tiao Codonopsis saponin has the strongest FRAP iron-reducing antioxidant capacity at a concentration of 300 ug / ml, with an iron-reducing antioxidant capacity of 79% ± 1.4142. The Lu Codonopsis saponin has an iron-reducing antioxidant capacity value of 71% ± 1.4142 at this concentration, and the wild Codonopsis saponin has an iron-reducing antioxidant capacity value of 68.8% ± 2.1213 at a concentration of 300 ug / ml. The iron-reducing antioxidant capacity of Bai Tiao Codonopsis saponin is significantly different from that of wild Codonopsis saponin, and *p < 0.05.

[0080] Codonopsis saponin can effectively scavenge free radicals in the body, reduce oxidative stress, and thus protect cells from damage. Codonopsis with low saponin content has relatively weak antioxidant activity.

[0081] Example Three:

[0082] The embodiment is basically the same as the previous embodiment, and the difference is that the processing treatment is also soil frying, and the processing parameters are as follows: 25-35% of the hearth soil by weight of the radix codonopsis decoction pieces is placed in a pot and fried until flexible, the decoction pieces are put in, and the decoction pieces are taken out when the surface of the decoction pieces is dark yellow and the pot smells of burnt aroma, and no additional trace element Mn is added.

[0083] Among them: 30 kg of soil per 100 kg of radix codonopsis decoction pieces.

[0084] The radix codonopsis is processed by the traditional rice frying method: the rice is placed in a frying pan and heated with a medium fire until the rice smokes, then the radix codonopsis pieces are put in and fried, and the radix codonopsis is taken out when it turns yellow, the rice is sieved out, and it is cooled. Among them, 20 kg of rice is used per 100 kg of radix codonopsis.

[0085] The radix codonopsis obtained by the above processing treatment and the radix codonopsis obtained by the traditional rice frying method are respectively subjected to antioxidant activity determination, and the results show that the radix codonopsis processed by the soil frying method has stronger antioxidant activity; further determination shows that the content of trace element Mn with antioxidant activity in the soil-fried radix codonopsis is the highest.

[0086] The above only describes some exemplary embodiments of the present application in a descriptive manner, and it is needless to say that those skilled in the art can modify the described embodiments in various ways without departing from the spirit and scope of the present application. Therefore, the above drawings and descriptions are illustrative in nature and should not be understood as limiting the scope of protection of the claims of the present application.

Claims

1. A processing method based on the medicinal substances and activities of Codonopsis pilosula, characterized in that, include: S1. Take Codonopsis pilosula medicinal material and pre-process it to obtain Codonopsis pilosula slices; S2. The Codonopsis pilosula slices are processed by rice frying. The processing parameters are as follows: rice is selected as the auxiliary material according to the ratio of Codonopsis pilosula mass to auxiliary material mass of 100:

20. The rice is put into a frying pan and heated over medium heat until it smokes. Then the Codonopsis pilosula slices are added and stir-fried for 8-12 minutes at 150-170℃ and 2.2-2.6kW until the Codonopsis pilosula turns slightly yellow and has a fragrant aroma. S3. After stir-frying, remove the auxiliary materials from the Codonopsis pilosula and let it cool to room temperature to obtain the processed Codonopsis pilosula product.

2. The processing method based on the medicinal substances and activities of Codonopsis pilosula according to claim 1, characterized in that, The processing method is also soil frying. The processing parameters are as follows: place the hearth soil, which is 25-35% of the weight of the Codonopsis pilosula slices, in a pot and fry until it becomes flexible. Then add the slices and fry until the surface of the slices is dark yellow and has a burnt aroma.

3. The processing method based on the medicinal substances and activities of Codonopsis pilosula according to claim 1, characterized in that, The processing parameters for the rice stir-fry are: stir-frying temperature 160℃, stir-frying time 10min, and stir-frying power 2.4kW.

4. The processing method based on the medicinal substances and activities of Codonopsis pilosula according to claim 1, characterized in that, The Codonopsis pilosula medicinal material mentioned is the dried root of Codonopsis pilosula or Codonopsis pilosula var. chinensis.

5. The processing method based on the medicinal substances and activities of Codonopsis pilosula according to claim 4, characterized in that, The Codonopsis pilosula mentioned is the white-striped Codonopsis pilosula.

6. The processing method based on the medicinal substances and activities of Codonopsis pilosula according to claim 1, characterized in that, The pretreatment includes washing and cutting the Codonopsis pilosula medicinal material, and drying it until it is no longer sticky to the touch to obtain Codonopsis pilosula slices.

7. The processing method based on the medicinal substances and activities of Codonopsis pilosula according to claim 1, characterized in that, The processed Codonopsis pilosula product contains ≥0.013% codonoside, has an ABTS free radical scavenging rate ≥78% at a concentration of 100μg / mL, and a DPPH free radical scavenging rate ≥76%.

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