Traditional Chinese medicine anti-tumor preparation and preparation method thereof

Abstract

The invention belongs to the technical field of anti-tumor drugs, and particularly relates to a traditional Chinese medicine anti-tumor preparation as well as a preparation method and application thereof. The medicine is prepared from the following raw materials in parts by weight: 90 to 110 parts of rhizoma sparganii processed with vinegar, 90 to 110 parts of rhizoma curcumae processed with vinegar, 60 to 90 parts of trogopterus dung processed with vinegar, 55 to 65 parts of rheum officinale, 40 to 60 parts of rhizoma cyperi processed with vinegar, 60 to 90 parts of hawthorn, 30 to 35 parts of pericarpium citri reticulatae viride processed with vinegar, 30 to 35 parts of pericarpium citri reticulatae, 50 to 60 parts of fried medicated leaven, 50 to 60 parts of fried malt and 40 to 45 parts of fried semen pharbitidis. The self-composition and the corresponding preparation method disclosed by the invention are used for breaking blood stasis and eliminating diseases, removing food retention and detoxifying, activating blood, promoting qi circulation and removing stasis, promoting digestion and removing food stagnation, and regulating qi and regulating middle warmer. The compound has a good inhibition effect on gastric cancer and colon cancer cells.

Description

Technical Field

[0001] This invention belongs to the field of antitumor drug technology, specifically relating to a traditional Chinese medicine antitumor preparation, its preparation method, and its application. Background Technology

[0002] Tumors are new growths formed by the abnormal proliferation of cells in the body. Their pathogenesis is complex, involving multiple factors such as genetics, environment, and lifestyle. Genetic factors such as gene mutations and family history can increase the risk; environmental factors include smoking, alcohol consumption, radiation, and chemical carcinogens; biological factors such as viral infections like HPV and EBV are also associated with various tumors; in addition, lifestyle factors such as chronic inflammation, immunosuppression, obesity, long-term poor diet, and lack of exercise are also important contributing factors.

[0003] Tumors destroy normal tissue structures, compress or invade surrounding organs, leading to functional impairments. For example, lung cancer causes breathing difficulties, brain tumors cause neurological deficits, and stomach and colon cancer restrict digestive function. Malignant tumors are metastatic, spreading throughout the body via the blood and lymphatic system, forming multiple metastatic lesions, greatly increasing the difficulty of treatment. Tumors also cause systemic wasting, leading to weight loss, anemia, fatigue, and other cachexia symptoms, severely impacting quality of life. Some tumors secrete abnormal hormones or cytokines, triggering paraneoplastic syndromes, causing endocrine disorders or neurological symptoms. Therefore, intervention measures for tumors are crucial.

[0004] Current anti-tumor treatments are mainly divided into Western targeted chemotherapy and traditional Chinese medicine (TCM) based on syndrome differentiation. Western medicine primarily works by damaging DNA and inhibiting metabolism, offering rapid onset of action but also significant side effects. To reduce side effects, a series of TCM anti-tumor drugs have been developed, including compound Kushen injection for clearing heat and detoxifying, Pingxiao capsules for chemically removing blood stasis, and Shenqi Fuzheng injection for strengthening the body's resistance. While TCM has fewer side effects, its effects are slower.

[0005] In summary, the technical problem that the existing technology needs to solve is to provide a fast-acting traditional Chinese medicine anti-tumor preparation. Summary of the Invention

[0006] To address the aforementioned technical problems, this invention provides a traditional Chinese medicine anti-tumor preparation, its preparation method, and its application.

[0007] The purpose of this invention is to provide a traditional Chinese medicine anti-tumor preparation, made from the following raw materials in parts by weight: 90-110 parts of vinegar-processed Sparganium rhizome, 90-110 parts of vinegar-processed Curcuma zedoaria rhizome, 60-90 parts of vinegar-processed Trogopterus xanthipes, 55-65 parts of rhubarb, 40-60 parts of vinegar-processed Cyperus rotundus rhizome, 60-90 parts of hawthorn fruit, 30-35 parts of vinegar-processed Citrus reticulata peel, 30-35 parts of dried tangerine peel, 50-60 parts of stir-fried Shenqu (medicated leaven), 50-60 parts of stir-fried malt, and 40-45 parts of stir-fried Pharbitis nil seeds.

[0008] It should be noted that the above traditional Chinese medicinal material is also commonly used in the art with the following corresponding names: Vinegar rhizoma polygonati corresponds to vinegar fried rhizoma polygonati, vinegar zedoary corresponds to vinegar fried zedoary, vinegar five musk corresponds to vinegar fried five musk, vinegar cyperus rotundus corresponds to vinegar fried cyperus rotundus, and vinegar cyperus rotundus corresponds to vinegar fried cyperus rotundus.

[0009] The innovation principle and solution of the above formula are as follows: 1. Monarch drug: vinegar rhizoma polygonati, vinegar zedoary, vinegar five musk.

[0010] Vinegar rhizoma polygonati and vinegar zedoary are countermeasures that break blood and promote qi, dissipate accumulation and relieve pain. They enter the liver meridian and blood system, break blood and dissipate accumulation, and can directly target the "accumulation of stagnation" (fixed mass) formed by tumors, and are the core drugs in the anti-tumor prescription of traditional Chinese medicine in the present application. The dosage of the two is set to 90-110 parts, which is used to break the blood stasis and dissipate the mass in the body.

[0011] Vinegar five musk: bitter and warm, enters the liver meridian. It is good at promoting blood circulation to remove blood stasis and relieving pain, and can also detoxify. Combined with vinegar rhizoma polygonati and vinegar zedoary, it can enhance the effect of breaking blood stasis and attacking stagnation, and relieve the pain caused by tumors.

[0012] 2. Minister drug: rhubarb, vinegar cyperus rotundus, hawthorn.

[0013] Rhubarb: bitter and cold, known as "general". In the composition of the present application, 55-65 parts are used, which can "dissipate blood stasis, break accumulation of stagnation", dredge the intestines and stomach, and promote the excretion of blood stasis and accumulation from the stool. Its purgation and blood circulation can assist the monarch drug in attacking evil.

[0014] Vinegar cyperus rotundus: soothing liver and regulating qi, regulating menstruation and relieving pain. After vinegar frying, its ability to soothe liver and relieve depression, regulate qi and relieve pain is more specialized. Qi is the commander of blood, and qi movement promotes blood movement. Vinegar cyperus rotundus is used to regulate qi to assist blood circulation, so that the monarch drug can break blood stasis smoothly, which is an important link to address "qi stagnation", a tumor pathogenesis.

[0015] Hawthorn: not only digest food, but also promote blood circulation and remove blood stasis, especially good at dissipating meat accumulation and gynecological blood stasis. In this prescription, its "dissolving blood stasis" effect is used to assist the monarch drug in dissipating blood stasis, while taking into account the transportation and transformation of the spleen and stomach.

[0016] In the preparation process of the present application, vinegar rhizoma polygonati, vinegar zedoary, vinegar five musk, rhubarb, vinegar cyperus rotundus and hawthorn are mixed and crushed into powder, and all the components are taken into medicine to fully exert the powerful anti-tumor effect of monarch and minister.

[0017] 3. Assistant drug: vinegar cyperus rotundus, dried tangerine or orange peel, fried six gods, fried wheat.

[0018] Vinegar green skin, dried tangerine peel: vinegar green skin broken gas and accumulation, dried tangerine peel regulating qi and invigorating the spleen. Both of them are used together, and the liver and spleen qi machine is loose and smooth, and the distension caused by qi stagnation is solved. At the same time, the qi regulating medicine can prevent the stagnation caused by a large amount of blood breaking medicine.

[0019] Fried six gods, fried wheat: after frying, the function of strengthening the spleen and stomach is enhanced. In the composition of the present invention, a large amount of broken gas attacking products can easily damage the spleen and stomach, so two drugs are used to protect the middle jiao, so that the evil can be attacked without damaging the normal, and the function of the acquired constitution is guaranteed. At the same time, they can digest the food accumulation that may be produced due to the weakening of the function of the spleen and stomach.

[0020] 4, make medicine: fried morning glory seeds.

[0021] Fried morning glory seeds: bitter and cold, toxic. It has the functions of purgation, water, accumulation and insect killing. In the composition of the present invention, the dosage is as small as 40 parts to 45 parts, because of its strong and powerful medicinal effect. Its role is:

[0022] (1) lead the way: lead the power of various drugs down, and make the blood stasis, toxins and accumulation have a way out.

[0023] (2) synergistic accumulation: synergistic with rhubarb, enhancing the effect of purgation, purgation and accumulation.

[0024] (3) control the dosage: because it is toxic and has strong purgation, the dosage needs to be strictly controlled to avoid excessive consumption of normal qi.

[0025] In the preparation process of the present invention, vinegar green skin, dried tangerine peel, fried six gods, fried wheat and fried morning glory seeds are mixed, 8 times to 10 times the mass of water is added, boiled for 25 min to 30 min, filtered, dried to obtain water extract, which is water decoction extraction process. The present invention takes the water extract of these medicines, which is used with the monarch and minister medicines to form a three-dimensional evil attacking system.

[0026] In summary, the self-formulation of the present invention has the following overall compatibility characteristics and effects: 1. Compatibility characteristics: the composition takes "breaking blood and removing tumor" as the core, and uses rhizoma zedoariae and curcuma as the monarch to concentrate firepower on attacking tumor lesions (tumor). At the same time, blood circulation, qi movement and other drugs are used as a supporting role, and spleen and stomach protecting drugs are used to protect the spleen and stomach, and morning glory seeds are used to lead the evil out. Form a three-dimensional evil attacking system of "breaking blood, moving qi, removing stagnation and supporting the middle".

[0027] 2. Effect: breaking blood and removing tumor, moving qi and relieving pain, attacking accumulation and detoxification. It is suitable for tumors of qi stagnation and blood stasis, phlegm and blood stasis type, with hard mass, stabbing pain, dark tongue, pulse and other symptoms. It can enhance the body's immunity, resist the invasion of external evil, and achieve the effect of resisting tumor. It has different degrees of inhibitory effect on gastric cancer, colon cancer and melanoma cells.

[0028] Preferably, the above-mentioned traditional Chinese medicine anti-tumor preparation is made of the following raw materials by weight: Eclipta prostrata L. 90 parts, Rhizoma Curcumae 90 parts, Myristicae Fructus 60 parts, Radix et Rhizoma Rhei 65 parts, Rhizoma Atractylodis 60 parts, Fructus Crataegi 90 parts, Pericarpium Citri Reticulatae Viride 30 parts, Pericarpium Citri Reticulatae 30 parts, Stir-baked Massa Medicata Fermentata 60 parts, Stir-baked Hordei Fructus 60 parts and Stir-baked Pharbitidis Semen 45 parts.

[0029] Preferably, the above-mentioned traditional Chinese medicine anti-tumor preparation is made of the following raw materials by weight: Eclipta prostrata L. 90 parts, Rhizoma Curcumae 90 parts, Myristicae Fructus 60 parts, Radix et Rhizoma Rhei 65 parts, Rhizoma Atractylodis 60 parts, Fructus Crataegi 90 parts, Pericarpium Citri Reticulatae Viride 30 parts, Pericarpium Citri Reticulatae 30 parts, Stir-baked Massa Medicata Fermentata 60 parts, Stir-baked Hordei Fructus 60 parts and Stir-baked Pharbitidis Semen 45 parts.

[0030] Preferably, the above-mentioned traditional Chinese medicine anti-tumor preparation is made of the following raw materials by weight: Eclipta prostrata L. 90 parts, Rhizoma Curcumae 90 parts, Myristicae Fructus 60 parts, Radix et Rhizoma Rhei 65 parts, Rhizoma Atractylodis 60 parts, Fructus Crataegi 90 parts, Pericarpium Citri Reticulatae Viride 30 parts, Pericarpium Citri Reticulatae 30 parts, Stir-baked Massa Medicata Fermentata 60 parts, Stir-baked Hordei Fructus 60 parts and Stir-baked Pharbitidis Semen 45 parts.

[0031] Preferably, the above-mentioned traditional Chinese medicine anti-tumor preparation is used for inhibiting the proliferation of gastric cancer cells MKN-45 and NCI-N87.

[0032] Preferably, the above-mentioned traditional Chinese medicine anti-tumor preparation is used for inhibiting the proliferation of colon cancer cells HT-29 and LS174T.

[0033] The application further provides a preparation method of the traditional Chinese medicine anti-tumor preparation. Eclipta prostrata L., Rhizoma Curcumae, Myristicae Fructus, Radix et Rhizoma Rhei, Rhizoma Atractylodis and Fructus Crataegi are mixed and crushed into powder; Pericarpium Citri Reticulatae Viride, Pericarpium Citri Reticulatae, Stir-baked Massa Medicata Fermentata, Stir-baked Hordei Fructus and Stir-baked Pharbitidis Semen are mixed, 8-10 times of water by mass is added, boiled for 25-30 minutes, filtered, dried to obtain water extract; The powder and the water extract are mixed to obtain the mixed medicine powder, which is the traditional Chinese medicine anti-tumor preparation.

[0034] Preferably, 0.03%-0.06% of sodium chloride by mass fraction of the mixed medicine powder is added to the mixed medicine powder, mixed to obtain a mixture, ethanol solution is added as film, water is added to granulate, dried to obtain the pill.

[0035] Preferably, the preparation method of the above-mentioned traditional Chinese medicine anti-tumor preparation, the volume fraction of the ethanol solution is 50%, and the amount of the ethanol solution added is 1%-2% of the mass of the mixture.

[0036] Preferably, the preparation method of the above-mentioned traditional Chinese medicine anti-tumor preparation, after water granulation, sieving is performed, the diameter of the sieve hole is 3.5 mm, the wet pills with a weight of 0.9-1.0 g per 20 pills are obtained, and then drying is performed.

[0037] The recommended use method of the traditional Chinese medicine antitumor preparation or pill of the present application is to take 2g-10g of the medicine each time, once in the morning and once in the evening, every day, and to observe the curative effect after taking 4 treatment courses of 7 days each.

[0038] Compared with the prior art, the present application has the following beneficial effects: The self-assembly formula of the present application is made from the following raw materials by weight: vinegar rhizoma polygonati 90-110 parts, vinegar rhizoma zedoariae 90-110 parts, vinegar wulingzhi 60-90 parts, rhubarb 55-65 parts, vinegar rhizoma zedoariae 40-60 parts, hawthorn 60-90 parts, vinegar pericarpium citri reticulatae 30-35 parts, dried tangerine or orange peel 30-35 parts, fried six gods 50-60 parts, fried malt 50-60 parts, and fried ipomoea 40-45 parts. The vinegar rhizoma polygonati, vinegar rhizoma zedoariae, vinegar wulingzhi, rhubarb, vinegar rhizoma zedoariae, and hawthorn are mixed and crushed into powder. The vinegar pericarpium citri reticulatae, dried tangerine or orange peel, fried six gods, fried malt, and fried ipomoea are mixed, water is added and boiled to extract, and the water extract is obtained. The powder and the water extract are mixed to obtain the mixed medicine powder, which is the traditional Chinese medicine antitumor preparation. It breaks blood and removes stasis, promotes qi and relieves pain, and attacks accumulation and detoxifies. It is suitable for tumors of the type of qi stagnation and blood stasis and phlegm and blood stasis, with symptoms such as hard lumps, stabbing pain, dark purple tongue, and tight pulse. It has good inhibitory effect on gastric cancer and colon cancer cells.

[0039] In addition, the preparation method of the traditional Chinese medicine antitumor preparation of the present application includes the following steps: the vinegar rhizoma polygonati, vinegar rhizoma zedoariae, vinegar wulingzhi, rhubarb, vinegar rhizoma zedoariae, and hawthorn are mixed and crushed into powder; the vinegar pericarpium citri reticulatae, dried tangerine or orange peel, fried six gods, fried malt, and fried ipomoea are mixed, 8-10 times the mass of water is added, boiled for 25-30 minutes, filtered, dried, and the water extract is obtained; the powder and the water extract are mixed, and the mixed medicine powder is obtained. Sodium chloride equivalent to 0.03%-0.06% of the mass fraction of the mixed medicine powder is added, mixed to obtain a mixture, and ethanol solution is added as a film. The water is filled into the pills, dried, and the traditional Chinese medicine antitumor preparation is obtained. Through crushing, all active substances in the vinegar rhizoma polygonati, vinegar rhizoma zedoariae, vinegar wulingzhi, rhubarb, vinegar rhizoma zedoariae, and hawthorn are retained. Then, the effective components in the vinegar pericarpium citri reticulatae, dried tangerine or orange peel, fried six gods, fried malt, and fried ipomoea are extracted by the traditional boiling water decoction process. After mixing with sodium chloride, the active components are maintained in an effective form, ensuring the efficacy of the medicine. Finally, the pills are made into water-filled pills, which are convenient for packaging and taking. BRIEF DESCRIPTION OF DRAWINGS

[0040] Figure 1 The cell death rates of Example 1 group.

[0041] Figure 2 The cell death rates of Example 2 group.

[0042] Figure 3Cell death rate of each group for Example 1.

[0043] Figure 4 Cell death rate of each group for Example 4.

[0044] Figure 5 Cell death rate of each group for Example 5.

[0045] Figure 6 Cell death rate of each group for Example 6.

[0046] Figure 7 Cell death rate of each group for Example 7.

[0047] Figure 8 Cell death rate of each group for Example 8.

[0048] Figure 9 Cell death rate of each group for Control Group 1.

[0049] Figure 10 Cell death rate of each group for Control Group 2.

[0050] Figure 11 Cell death rate of each group for Control Group 3.

[0051] Figure 12 Cell death rate of each group for Control Group 4.

[0052] Figure 13 Cell death rate of each group for Control Group 5.

[0053] Figure 14 Cell death rate of each group for Control Group 6.

[0054] Figure 15 Cell death rate of each group for Control Group 7.

[0055] Figure 16 Cell death rate of each group for Control Group 8. DETAILED DESCRIPTION

[0056] In order to make the skilled in the art better understand the technical solutions of the present application can be implemented, the following specific examples of the present application is further described.

[0057] In the description of the present application, if not special, the use of reagents are commercially available, the use of methods are conventional techniques in the art.

[0058] In the following examples and control groups, the temperature of water soaking and standing extraction is room temperature 25℃.

[0059] I. Different formula settings.

[0060] Example 1 A traditional Chinese medicine antitumor preparation is made from the following raw materials by weight: vinegar rhizoma polygonati 90 parts, vinegar zedoary turmeric 90 parts, vinegar five spirit fat 60 parts, rhubarb 65 parts, vinegar cyperus 60 parts, hawthorn 90 parts, vinegar green skin 30 parts, dried tangerine or orange peel 30 parts, fried six god's recipe 60 parts, fried wheat 60 parts and fried morning glory seeds 45 parts.

[0061] The preparation method comprises the following steps: The vinegar rhizoma polygonati, vinegar zedoary turmeric, vinegar five spirit fat, rhubarb, vinegar cyperus and hawthorn are mixed and crushed into fine powder of 120 mesh; The vinegar green skin, dried tangerine or orange peel, fried six god's recipe, fried wheat and fried morning glory seeds are mixed, 8 times the mass of water is added, boiled for 25 minutes, filtered through a 100 mesh filter, and dried at 45°C to obtain a water extract; The fine powder and the water extract are mixed to obtain a mixed medicinal powder, and the traditional Chinese medicine antitumor preparation is obtained.

[0062] Example 2 A traditional Chinese medicine antitumor preparation is made from the following raw materials by weight: vinegar rhizoma polygonati 110 parts, vinegar zedoary turmeric 110 parts, vinegar five spirit fat 90 parts, rhubarb 60 parts, vinegar cyperus 60 parts, hawthorn 90 parts, vinegar green skin 35 parts, dried tangerine or orange peel 35 parts, fried six god's recipe 50 parts, fried wheat 50 parts and fried morning glory seeds 40 parts.

[0063] The preparation method comprises the following steps: The vinegar rhizoma polygonati, vinegar zedoary turmeric, vinegar five spirit fat, rhubarb, vinegar cyperus and hawthorn are mixed and crushed into fine powder of 120 mesh; The vinegar green skin, dried tangerine or orange peel, fried six god's recipe, fried wheat and fried morning glory seeds are mixed, 8 times the mass of water is added, boiled for 25 minutes, filtered through a 100 mesh filter, and dried at 45°C to obtain a water extract; The fine powder and the water extract are mixed to obtain a mixed medicinal powder, and the traditional Chinese medicine antitumor preparation is obtained.

[0064] Example 3 A traditional Chinese medicine antitumor preparation is made from the following raw materials by weight: vinegar rhizoma polygonati 100 parts, vinegar zedoary turmeric 100 parts, vinegar five spirit fat 70 parts, rhubarb 55 parts, vinegar cyperus 40 parts, hawthorn 60 parts, vinegar green skin 30 parts, dried tangerine or orange peel 30 parts, fried six god's recipe 60 parts, fried wheat 60 parts and fried morning glory seeds 45 parts.

[0065] The preparation method comprises the following steps: The vinegar rhizoma polygonati, vinegar zedoary turmeric, vinegar five spirit fat, rhubarb, vinegar cyperus and hawthorn are mixed and crushed into fine powder of 120 mesh; The vinegar green skin, dried tangerine or orange peel, fried six god's recipe, fried wheat and fried morning glory seeds are mixed, 8 times the mass of water is added, boiled for 25 minutes, filtered through a 100 mesh filter, and dried at 45°C to obtain a water extract; The fine powder and the water extract are mixed to obtain a mixed medicinal powder, and the traditional Chinese medicine antitumor preparation is obtained.

[0066] Example 4 A traditional Chinese medicine anti-tumor preparation is made from the following raw materials by weight: vinegar three collinear 90 parts, vinegar atractylodes 90 parts, vinegar five Lingzhi 60 parts, rhubarb 65 parts, vinegar Cyperus 60 parts, hawthorn 90 parts, vinegar green skin 30 parts, dried tangerine or orange peel 30 parts, fried six Shenqu 60 parts, fried wheat 60 parts and fried morning glory seeds 45 parts.

[0067] The preparation method comprises the following steps: After mixing vinegar three collinear, vinegar atractylodes, vinegar five Lingzhi, rhubarb, vinegar Cyperus and hawthorn, they are crushed into fine powder of 120 mesh; After mixing vinegar green skin, dried tangerine or orange peel, fried six Shenqu, fried wheat and fried morning glory seeds, 8 times the mass of water is added, boiled for 25 minutes, filtered through a 100 mesh filter, and dried at 45°C to obtain a water extract; Mix the fine powder with the water extract, add sodium chloride equivalent to 0.03% of the mass fraction of the mixed medicine powder, and mix to obtain a mixture, which is the traditional Chinese medicine anti-tumor preparation.

[0068] Example 5 A traditional Chinese medicine anti-tumor preparation is made from the following raw materials by weight: vinegar three collinear 90 parts, vinegar atractylodes 90 parts, vinegar five Lingzhi 60 parts, rhubarb 65 parts, vinegar Cyperus 60 parts, hawthorn 90 parts, vinegar green skin 30 parts, dried tangerine or orange peel 30 parts, fried six Shenqu 60 parts, fried wheat 60 parts and fried morning glory seeds 45 parts.

[0069] The preparation method comprises the following steps: After mixing vinegar three collinear, vinegar atractylodes, vinegar five Lingzhi, rhubarb, vinegar Cyperus and hawthorn, they are crushed into fine powder of 120 mesh; After mixing vinegar green skin, dried tangerine or orange peel, fried six Shenqu, fried wheat and fried morning glory seeds, 8 times the mass of water is added, boiled for 25 minutes, filtered through a 100 mesh filter, and dried at 45°C to obtain a water extract; Mix the fine powder with the water extract, add sodium chloride equivalent to 0.03% of the mass fraction of the mixed medicine powder, and mix to obtain a mixture, which is the traditional Chinese medicine anti-tumor preparation.

[0070] The volume fraction of the ethanol solution is 50%, and the amount of ethanol solution added is 1% of the mass of the mixture.

[0071] Adjust the monarch, minister, assistant and envoy relationship of Example 1 to explore the effect of different prescriptions on the reduction of belly fat. The control group is set as follows: Control group 1 A traditional Chinese medicine antitumor preparation, only containing the monarch drug and the ministerial drug of example 1, is prepared from the following raw materials by weight: vinegar rhizoma sparganii 90 parts, vinegar zedoary turmeric 90 parts, vinegar five Lingzhi 60 parts, rhubarb 65 parts, vinegar Cyperus 60 parts, hawthorn 90 parts.

[0072] The preparation method comprises the following steps: After the vinegar rhizoma sparganii, vinegar zedoary turmeric, vinegar five Lingzhi, rhubarb, vinegar Cyperus, and hawthorn are mixed, they are crushed into fine powder of 120 mesh.

[0073] Control group 2 A traditional Chinese medicine antitumor preparation, only containing the monarch drug, the ministerial drug and the auxiliary drug of example 1, has the following formula: vinegar rhizoma sparganii 90 parts, vinegar zedoary turmeric 90 parts, vinegar five Lingzhi 60 parts, rhubarb 65 parts, vinegar Cyperus 60 parts, hawthorn 90 parts, vinegar green skin 30 parts, dried tangerine or orange peel 30 parts, fried six God's recipe 60 parts, fried wheat 60 parts.

[0074] The preparation method comprises the following steps: After the vinegar rhizoma sparganii, vinegar zedoary turmeric, vinegar five Lingzhi, rhubarb, vinegar Cyperus, and hawthorn are mixed, they are crushed into fine powder of 120 mesh. After the vinegar rhizoma sparganii, vinegar zedoary turmeric, vinegar five Lingzhi, rhubarb, vinegar Cyperus, and hawthorn are mixed, they are crushed into fine powder of 120 mesh. The fine powder and the water extract are mixed to obtain a mixed medicine powder, and the traditional Chinese medicine antitumor preparation is obtained.

[0075] Control group 3 A traditional Chinese medicine antitumor preparation, only containing the monarch drug, the ministerial drug and the auxiliary drug of example 1, has the following formula: vinegar rhizoma sparganii 90 parts, vinegar zedoary turmeric 90 parts, vinegar five Lingzhi 60 parts, rhubarb 65 parts, vinegar Cyperus 60 parts, hawthorn 90 parts and fried morning glory seeds 45 parts.

[0076] The preparation method comprises the following steps: After the vinegar rhizoma sparganii, vinegar zedoary turmeric, vinegar five Lingzhi, rhubarb, vinegar Cyperus, and hawthorn are mixed, they are crushed into fine powder of 120 mesh. After the vinegar rhizoma sparganii, vinegar zedoary turmeric, vinegar five Lingzhi, rhubarb, vinegar Cyperus, and hawthorn are mixed, they are crushed into fine powder of 120 mesh. The fine powder and the water extract are mixed to obtain a mixed medicine powder, and the traditional Chinese medicine antitumor preparation is obtained.

[0077] II. Setting of different boiling conditions in the preparation method

[0078] Example 6 A traditional Chinese medicine antitumor preparation is made from the following raw materials by weight: vinegar rhizoma polygonati 90 parts, vinegar zedoary turmeric 90 parts, vinegar five Lingzhi 60 parts, rhubarb 65 parts, vinegar Cyperus 60 parts, hawthorn 90 parts, vinegar green skin 30 parts, dried tangerine or orange peel 30 parts, fried six God's recipe 60 parts, fried wheat 60 parts and fried morning glory seeds 45 parts.

[0079] The preparation method comprises the following steps: The vinegar rhizoma polygonati, vinegar zedoary turmeric, vinegar five Lingzhi, rhubarb, vinegar Cyperus and hawthorn are mixed and crushed into fine powder of 120 mesh; The vinegar green skin, dried tangerine or orange peel, fried six God's recipe, fried wheat and fried morning glory seeds are mixed, 10 times the mass of water is added, boiled for 25 minutes, filtered through a 100 mesh filter, and dried at 45°C to obtain a water extract; The fine powder and the water extract are mixed to obtain a mixed medicinal powder, which is the traditional Chinese medicine antitumor preparation.

[0080] Example 7 A traditional Chinese medicine antitumor preparation is made from the following raw materials by weight: vinegar rhizoma polygonati 90 parts, vinegar zedoary turmeric 90 parts, vinegar five Lingzhi 60 parts, rhubarb 65 parts, vinegar Cyperus 60 parts, hawthorn 90 parts, vinegar green skin 30 parts, dried tangerine or orange peel 30 parts, fried six God's recipe 60 parts, fried wheat 60 parts and fried morning glory seeds 45 parts.

[0081] The preparation method comprises the following steps: The vinegar rhizoma polygonati, vinegar zedoary turmeric, vinegar five Lingzhi, rhubarb, vinegar Cyperus and hawthorn are mixed and crushed into fine powder of 120 mesh; The vinegar green skin, dried tangerine or orange peel, fried six God's recipe, fried wheat and fried morning glory seeds are mixed, 8 times the mass of water is added, boiled for 30 minutes, filtered through a 100 mesh filter, and dried at 45°C to obtain a water extract; The fine powder and the water extract are mixed to obtain a mixed medicinal powder, which is the traditional Chinese medicine antitumor preparation.

[0082] Control group 4 A traditional Chinese medicine antitumor preparation is made from the following raw materials by weight: vinegar rhizoma polygonati 90 parts, vinegar zedoary turmeric 90 parts, vinegar five Lingzhi 60 parts, rhubarb 65 parts, vinegar Cyperus 60 parts, hawthorn 90 parts, vinegar green skin 30 parts, dried tangerine or orange peel 30 parts, fried six God's recipe 60 parts, fried wheat 60 parts and fried morning glory seeds 45 parts.

[0083] The preparation method comprises the following steps: The vinegar rhizoma polygonati, vinegar zedoary turmeric, vinegar five Lingzhi, rhubarb, vinegar Cyperus and hawthorn are mixed and crushed into fine powder of 120 mesh; The vinegar green skin, dried tangerine or orange peel, fried six God's recipe, fried wheat and fried morning glory seeds are mixed, 8 times the mass of water is added, boiled for 20 minutes, filtered through a 100 mesh filter, and dried at 45°C to obtain a water extract; The fine powder and the water extract are mixed to obtain a mixed medicinal powder, which is the traditional Chinese medicine antitumor preparation.

[0084] Control group 5 A traditional Chinese medicine antitumor preparation is made from the following raw materials by weight: vinegar three collinear 90 parts, vinegar atractylodes 90 parts, vinegar five Lingzhi 60 parts, rhubarb 65 parts, vinegar Cyperus 60 parts, hawthorn 90 parts, vinegar green skin 30 parts, dried tangerine or orange peel 30 parts, fried six God's recipe 60 parts, fried wheat 60 parts and fried morning glory seeds 45 parts.

[0085] The preparation method comprises the following steps: After mixing vinegar three collinear, vinegar atractylodes, vinegar five Lingzhi, rhubarb, vinegar Cyperus and hawthorn, they are crushed into fine powder of 120 mesh; After mixing vinegar green skin, dried tangerine or orange peel, fried six God's recipe, fried wheat and fried morning glory seeds, 8 times the mass of water is added, boiled for 40 minutes, filtered through a 100 mesh filter, and dried at 45°C to obtain a water extract; Mix the fine powder with the water extract, and the obtained mixed medicine powder is the traditional Chinese medicine antitumor preparation.

[0086] III. Settings of different reagents in the mixed medicine powder combination in the preparation method.

[0087] Example 8 A traditional Chinese medicine antitumor preparation is made from the following raw materials by weight: vinegar three collinear 90 parts, vinegar atractylodes 90 parts, vinegar five Lingzhi 60 parts, rhubarb 65 parts, vinegar Cyperus 60 parts, hawthorn 90 parts, vinegar green skin 30 parts, dried tangerine or orange peel 30 parts, fried six God's recipe 60 parts, fried wheat 60 parts and fried morning glory seeds 45 parts.

[0088] The preparation method comprises the following steps: After mixing vinegar three collinear, vinegar atractylodes, vinegar five Lingzhi, rhubarb, vinegar Cyperus and hawthorn, they are crushed into fine powder of 120 mesh; After mixing vinegar green skin, dried tangerine or orange peel, fried six God's recipe, fried wheat and fried morning glory seeds, 8 times the mass of water is added, boiled for 40 minutes, filtered through a 100 mesh filter, and dried at 45°C to obtain a water extract; Mix the fine powder with the water extract, and the obtained mixed medicine powder is the traditional Chinese medicine antitumor preparation.

[0089] Control group 6 A traditional Chinese medicine antitumor preparation is made from the following raw materials by weight: vinegar three collinear 90 parts, vinegar atractylodes 90 parts, vinegar five Lingzhi 60 parts, rhubarb 65 parts, vinegar Cyperus 60 parts, hawthorn 90 parts, vinegar green skin 30 parts, dried tangerine or orange peel 30 parts, fried six God's recipe 60 parts, fried wheat 60 parts and fried morning glory seeds 45 parts.

[0090] The preparation method comprises the following steps: After mixing vinegar three collinear, vinegar atractylodes, vinegar five Lingzhi, rhubarb, vinegar Cyperus and hawthorn, they are crushed into fine powder of 120 mesh; After mixing vinegar-processed green tangerine peel, dried tangerine peel, stir-fried Shenqu (a type of fermented medicinal herb), stir-fried malt, and stir-fried morning glory seeds, add 8 times the weight of water, boil for 25 minutes, filter through a 100-mesh sieve, and dry at 45°C to obtain an aqueous extract. The fine powder is mixed with the water extract to obtain a mixed powder. Then, sodium chloride at a mass fraction of 0.09% is added to the mixed powder and mixed to obtain a mixture, which is a traditional Chinese medicine anti-tumor preparation.

[0091] IV. Traditional Water Extraction Process Control group 7 A traditional Chinese medicine antitumor preparation is made from the following raw materials in parts by weight: 90 parts of vinegar-processed Sparganium rhizome, 90 parts of vinegar-processed Curcuma zedoaria rhizome, 60 parts of vinegar-processed Trogopterus xanthipes, 65 parts of rhubarb, 60 parts of vinegar-processed Cyperus rhizome, 90 parts of hawthorn fruit, 30 parts of vinegar-processed Citrus reticulata peel, 30 parts of dried tangerine peel, 60 parts of stir-fried Shenqu (medicated leaven), 60 parts of stir-fried malt, and 45 parts of stir-fried Pharbitis nil.

[0092] The preparation method includes the following steps: After mixing all the ingredients, add water equivalent to 8 times the total mass of the mixture and boil for 25 minutes. Filter through a 100-mesh sieve and dry at 45°C to obtain the decoction.

[0093] V. Ethanol Solution Extraction Process control group 8 A traditional Chinese medicine antitumor preparation is made from the following raw materials in parts by weight: 90 parts of vinegar-processed Sparganium rhizome, 90 parts of vinegar-processed Curcuma zedoaria rhizome, 60 parts of vinegar-processed Trogopterus xanthipes, 65 parts of rhubarb, 60 parts of vinegar-processed Cyperus rhizome, 90 parts of hawthorn fruit, 30 parts of vinegar-processed Citrus reticulata peel, 30 parts of dried tangerine peel, 60 parts of stir-fried Shenqu (medicated leaven), 60 parts of stir-fried malt, and 45 parts of stir-fried Pharbitis nil.

[0094] The preparation method includes the following steps: After mixing all the raw materials, add 35% ethanol solution with a volume fraction equivalent to 8 times the total mass of the mixture, let stand for 2 hours for extraction, filter through a 100-mesh filter, and dry the collected filtrate at 45°C to obtain the drug for abdominal fat.

[0095] The effect verification process is as follows.

[0096] (1) Cells: Gastric cancer cells: Human gastric cancer cells MKN-45 (Shanghai Kanglang Biotechnology Co., Ltd.), NCI-N87 human gastric cancer cells (Wuhan Punosei Life Technology Co., Ltd.).

[0097] Colon cancer cells: Human colon cancer cells HT-29 (Shanghai Kanglang Biotechnology Co., Ltd.), Human colon cancer cells LS174T (Shanghai Kanglang Biotechnology Co., Ltd.).

[0098] (2) Preparation of drug powder: The drugs in each example and the control group were ground into 120-mesh powder to obtain test drug powder.

[0099] (3) Cell death rate experiment All cells were cultured for 72 h in DMEM / F12 medium containing 10% fetal bovine serum (FBS). The culture conditions were: 37°C and 5% CO2. After culture, the concentration was adjusted to 10%. 5 Cell sap per mL.

[0100] Add 5 micrograms of the test drug powder to each milliliter of cell saturation and incubate at 37°C with 5% CO2 for 24 hours. Test every 6 hours. Count the number of viable cells and calculate the cell death rate using the following formula.

[0101] Cell death rate = 100% × (1 - number of surviving cells / original number of cells).

[0102] Use sterile water of equal mass to replace the test drug powder as a blank control.

[0103] The cell death rate results after 24 hours of incubation are shown in Table 1. Cell death rate curves for different incubation times are shown in [reference needed]. Figures 1-16 The results showed that Examples 1 to 8 exhibited high inhibitory activity against various cancer cells. Among them, Examples 4, 5, and 8, which contained 0.03% and 0.06% sodium chloride, showed the best inhibitory effect on cancer cells. This indicates that the formulations provided by this invention and their complexes with sodium chloride have good anti-tumor effects. Example 5 of this invention is an example of a water-based pill, and the results showed that this dosage form also has good anti-tumor effects. The preparation process of the water-based pill is simple, and the resulting drug is easy to package and store, making it of great application value.

[0104] Among the control groups, control group 1, lacking only the principal and assistant drugs, had insufficient active ingredients; control group 2 lacked the guiding drug; and control group 3 lacked the adjuvant drug, resulting in weak inhibitory activity against tumor cells. Control group 6 involved adjustments to the amount of sodium chloride, showing that different drug component dosages also affected the research results. Control groups 4 and 5 involved adjustments to the boiling conditions, while control groups 7 and 8 involved adjustments to the overall preparation process, showing that different preparation processes significantly affected the inhibitory effect on cancer cells.

[0105] Table 1 Cell mortality rate after 24 hours of incubation It should be noted that when the present application refers to a numerical range, it is understood that each numerical range has two endpoints and any number between the two endpoints can be selected. Since the same method of steps and examples are used, the preferred embodiments are described in the present application to prevent redundancy. Although the preferred embodiments of the present application have been described, those skilled in the art who have the inventive concept of the present application can make additional changes and modifications to the embodiments, and these changes and modifications fall within the scope of the present application.

[0106] Obviously, various modifications and changes can be made to the present application by those skilled in the art without departing from the spirit and scope of the present application. If these modifications and changes of the present application fall within the scope of the equivalent technology of the present application, the present application also intends to include these modifications and changes.

Claims

1. A traditional Chinese medicine antitumor preparation, characterized in that, It is made from the following raw materials in parts by weight: 90-110 parts of vinegar-processed Sparganium rhizome, 90-110 parts of vinegar-processed Curcuma rhizome, 60-90 parts of vinegar-processed Trogopterus xanthipes, 55-65 parts of rhubarb, 40-60 parts of vinegar-processed Cyperus rhizome, 60-90 parts of hawthorn, 30-35 parts of vinegar-processed Citrus reticulata peel, 30-35 parts of dried tangerine peel, 50-60 parts of stir-fried Shenqu (medicated leaven), 50-60 parts of stir-fried malt, and 40-45 parts of stir-fried Pharbitis nil. The anti-tumor treatment refers to treatment against gastric and colon cancer. Vinegar-processed Sparganium rhizome, vinegar-processed Curcuma rhizome, vinegar-processed Trogopterus dung, rhubarb, vinegar-processed Cyperus rhizome, and hawthorn are mixed and pulverized into powder. Vinegar-processed Citrus reticulata peel, dried tangerine peel, stir-fried Shenqu (medicated leaven), stir-fried malt, and stir-fried morning glory seeds are mixed and boiled with water to extract the aqueous extract. The powder and aqueous extract are then mixed to obtain a mixed powder, which is a traditional Chinese medicine anti-tumor preparation.

2. The antitumor preparation of traditional Chinese medicine according to claim 1, characterized in that, It is made from the following ingredients in parts by weight: 90 parts vinegar-processed Sparganium rhizome, 90 parts vinegar-processed Curcuma rhizome, 60 parts vinegar-processed Trogopterus xanthipes, 65 parts rhubarb, 60 parts vinegar-processed Cyperus rhizome, 90 parts hawthorn, 30 parts vinegar-processed Citrus reticulata peel, 30 parts dried tangerine peel, 60 parts stir-fried Shenqu (medicated leaven), 60 parts stir-fried malt, and 45 parts stir-fried Morning Glory Seed.

3. The antitumor preparation of traditional Chinese medicine according to claim 1, characterized in that, 110 parts of vinegar-processed Sparganium rhizome, 110 parts of vinegar-processed Curcuma rhizome, 90 parts of vinegar-processed Trogopterus xanthipes, 60 parts of rhubarb, 60 parts of vinegar-processed Cyperus rhizome, 90 parts of hawthorn, 35 parts of vinegar-processed Citrus reticulata peel, 35 parts of dried tangerine peel, 50 parts of stir-fried Shenqu (medicated leaven), 50 parts of stir-fried malt, and 40 parts of stir-fried Morning Glory Seed.

4. The antitumor preparation of traditional Chinese medicine according to claim 1, characterized in that, 100 parts of vinegar-processed Sparganium rhizome, 100 parts of vinegar-processed Curcuma rhizome, 70 parts of vinegar-processed Trogopterus xanthipes, 55 parts of rhubarb, 40 parts of vinegar-processed Cyperus rhizome, 60 parts of hawthorn, 30 parts of vinegar-processed Citrus reticulata peel, 30 parts of dried tangerine peel, 60 parts of stir-fried Shenqu (medicated leaven), 60 parts of stir-fried malt, and 45 parts of stir-fried Morning Glory Seed.

5. The antitumor preparation of traditional Chinese medicine according to claim 1, characterized in that, The term "anti-gastric cancer" refers to inhibiting the proliferation of gastric cancer cells MKN-45 and NCI-N87.

6. The antitumor preparation of traditional Chinese medicine according to claim 1, characterized in that, The term "anti-colon cancer" refers to inhibiting the proliferation of colon cancer cells HT-29 and LS174T.

7. The preparation method of the traditional Chinese medicine antitumor preparation according to any one of claims 1-6, characterized in that, Includes the following steps: Vinegar-processed Sparganium rhizome, vinegar-processed Curcuma rhizome, vinegar-processed Trogopterus dung, rhubarb, vinegar-processed Cyperus rhizome, and hawthorn fruit are mixed and then pulverized into powder. After mixing vinegar-processed green tangerine peel, dried tangerine peel, stir-fried Shenqu (a type of fermented medicinal yeast), stir-fried malt, and stir-fried morning glory seeds, add 8 to 10 times the weight of water, boil for 25 to 30 minutes, filter, and dry to obtain an aqueous extract. The powder is mixed with the water extract to obtain a mixed powder.

8. The method for preparing the traditional Chinese medicine antitumor preparation according to claim 7, characterized in that, Add sodium chloride at a mass fraction of 0.03% to 0.06% to the mixed powder, mix to obtain a mixture, add ethanol solution to form a film, make into pills with water, dry to obtain pills.

9. The preparation method according to claim 8, characterized in that, The volume fraction of the ethanol solution is 50%, and the amount of ethanol solution added is 1% to 2% of the mass of the mixture.

10. The method of claim 9, wherein, After being soaked in water, the pills are sieved to obtain pills weighing 0.9g to 1.0g for every 20 wet pills, which are then dried.

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