Method for preparing handmade soap through lipase catalysis

Abstract

The invention relates to the technical field of handmade soap preparation, in particular to a method for preparing handmade soap through lipase catalysis, which comprises the following steps: uniformly stirring molten grease and weak base powder to form a substrate mixture; adding an aqueous solution containing alkaline lipase into the substrate mixture, and continuously stirring uniformly to form a reaction mixture; the reaction mixture is firstly subjected to a reaction of catalyzing grease hydrolysis by alkaline lipase, and the hydrolysate fatty acid is then reacted with weak base to form fatty acid salt, so that saponification is realized; continuously stirring for 30 minutes until the reaction mixture is pasty, stopping stirring, and pouring into a mold; the soap body is packaged after being subjected to after-ripening, strong base is replaced with weak base in the production process, the saponification reaction is completed at normal temperature, and no safety risk exists in the manufacturing process. The saponification reaction catalyzed by lipase is thorough, the after-ripening time is remarkably shortened, no strong base is left in a soap body, and the soap is mild to a user and has no use risk. And enzymes such as lipase reserved in the soap body enhance the washing effect.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of handmade soap preparation, in particular to a method for preparing handmade soap by using lipase catalysis. BACKGROUND

[0002] Handmade soap is a special washing product with health care function in recent years. Because its raw material comes from natural animal and plant oil, the components are mild, the foam is rich and delicate, the skin is cleaned thoroughly and permeably, and long-term use will not cause burden, harm and allergy to the skin. The production of handmade soap is mainly completed by small and medium-sized enterprises or small family workshops, and cold process is mostly used. Cold process refers to slightly heating the oil to melt, mixing it with alkali, slowly reacting, and adding additives such as essential oil and pigment to adjust the quality of the soap during the reaction. Finally, the soap block is dried, cut and dried for 4-6 weeks at room temperature. Because the glycerol is completely preserved in the soap body during the cold process, its content accounts for one fourth of the entire soap. Glycerol is a natural moisturizing agent, so handmade soap is more moisturizing and hydrating than commercially available soap.

[0003] With the deepening of the concept of natural health and environmental protection, pure natural handmade soap is increasingly favored by young white-collar women, and more and more consumers will abandon artificial chemical cosmetics and choose pure natural care products. As a new bathing and skin care trend, the huge market potential of pure natural handmade soap makes it one of the golden industries. There are many brands of cleaning and skin care products on the market, which contain a large amount of chemical ingredients, not only harmful to the user's skin, but also not environmentally friendly. Consumers who advocate natural and healthy concepts begin to choose products with pure natural ingredients. Pure natural handmade soap is made of 100% natural materials and contains rich moisturizing ingredients such as natural glycerol and rich natural antioxidants, becoming a popular alternative to chemically made bath and skin care products around the world.

[0004] The addition of alkali in the cold process is the guarantee of saponification reaction and the key technology in the field of soap production. In the traditional cold process, strong alkali sodium hydroxide (NaOH) is added to realize the saponification reaction of oil to prepare soap base.

[0005] However, the prior art uses oil and sodium hydroxide as the main raw material to complete the saponification reaction, and a large amount of heat is released during the reaction process, which has safety risks in the production process. The residual sodium hydroxide in the soap body can burn the user's skin, which has safety risks in the use process. SUMMARY

[0006] The purpose of the present application is to provide a method for preparing handmade soap by using lipase catalysis to solve the problems in the background art.

[0007] To achieve the above purpose, the present application provides the following technical scheme: As an alternative to the lipase catalyzed method of making handmade soap, the method comprises the following steps: (1) mixing the molten oil and weak base powder to form a substrate mixture; (2) adding an aqueous solution containing alkaline lipase to the substrate mixture and continuing to stir to form a reaction mixture; the alkaline lipase catalyzes the hydrolysis of the oil in the reaction mixture, and the hydrolysis product, fatty acid, subsequently reacts with the weak base to form a fatty acid salt, thereby achieving saponification; (3) continuing to stir for 30 minutes until the reaction mixture becomes a paste, stopping the stirring, and pouring into a mold; (4) packaging after the soap body is finished and aged.

[0008] As an alternative to the lipase catalyzed method of making handmade soap, the method comprises the following steps:

[0009] As an alternative to the lipase catalyzed method of making handmade soap, the method comprises the following steps:

[0010] As an alternative to the lipase catalyzed method of making handmade soap, the method comprises the following steps:

[0011] As an alternative to the lipase catalyzed method of making handmade soap, the method comprises the following steps:

[0012] As an alternative to the lipase catalyzed method of making handmade soap, the method comprises the following steps:

[0013] As an alternative to the lipase catalyzed method of making handmade soap, the method comprises the following steps:

[0014] As an alternative to the lipase catalyzed method of making handmade soap, the method comprises the following steps:

[0015] Compared with the prior art, the present application has the following advantages: 1. The method according to the present application, the production process uses weak alkali instead of strong alkali, and the saponification reaction is completed at room temperature, so there is no safety risk in the production process.

[0016] 2. The lipase-catalyzed saponification reaction is complete, significantly shortening the ripening time, and there is no strong alkali residue in the soap body, which is mild to the user and has no use risk.

[0017] 3. The lipase and other enzymes retained in the soap body enhance the washing effect. DETAILED DESCRIPTION

[0018] The present application will be further described in conjunction with specific examples to better understand the content of the present application, but the present application is not limited to the following examples.

[0019] Example 1: Coconut oil soap (vegetable oil + sodium carbonate) 1. Raw material preparation: Take 1000g of coconut oil (saponification value: 185mg NaOH / g), heat to 30℃ to completely melt. Calculate the required sodium carbonate (weak base) according to the saponification value: The number of moles of fatty acids to be neutralized=(1000g×185mg NaOH / g) / (40000mg NaOH / mol)≈4.625mol The required mass of sodium carbonate (Na2CO3, MW 106)=(4.625mol / 2)×106g / mol≈245g; 2. Substrate mixing: Add 245g of sodium carbonate powder to the melted coconut oil, stir well to form a substrate mixture.

[0020] 3. Enzyme solution preparation and addition: Take 10g of single alkaline lipase preparation produced by microbial fermentation (enzyme activity≥10,000U / g), dissolve in 190g of deionized water to prepare a 5% (w / w) enzyme-containing aqueous solution. Add this enzyme solution (total 200g) to the substrate mixture of step 2 (enzyme solution addition amount is about 16.7% of the mass of the substrate mixture).

[0021] 4. Enzyme-catalyzed reaction: Continue to stir the mixture, and the following reactions occur: (1) Alkaline lipase-catalyzed oil hydrolysis reaction: (RCOO)3C3H5+3H2O 3RCOOH+C3H5(OH)3 (2) Neutralization reaction of fatty acids and sodium carbonate: 6RCOOH+3Na2CO3→6RCOONa+3H2O+3CO2 1. Reaction process observation: The mixture gradually thickens Creation of small amount of bubbles (CO2 release) Temperature maintained at 30-35 °C (exothermic reaction) Reaction end point determination: After about 60 minutes of continuous stirring, the mixture thickened significantly to a paste, with a clear trace that was not easily erased by a spatula, and stirring was stopped.

[0022] Post-maturation: The paste was transferred to a mold and allowed to post-mature at room temperature (25 °C) for 3 days, completing the saponification process.

[0023] The principles and implementation modes of the present application are described herein using specific examples, and the above examples are only used to help understand the method of the present application and its core idea. The above description is only a preferred embodiment of the present application. It should be noted that due to the limited nature of the written expression, there are objectively infinite specific structures, and for ordinary skilled persons in the technical field, without departing from the principles of the present application, a number of improvements, refinements or changes can be made, or the above technical features can be combined in an appropriate manner; these improvements, refinements, changes or combinations, or the direct application of the inventive concept and technical solution to other occasions without improvement, shall be regarded as the protection scope of the present application.

Claims

1. A method for producing handmade soap using lipase catalysis, characterized in that, Includes the following steps: (1) Mix the molten oil and weak base powder evenly to form a substrate mixture; (2) Add an aqueous solution containing alkaline lipase to the above substrate mixture and continue stirring until homogeneous to form a reaction mixture; the alkaline lipase-catalyzed hydrolysis of oils first occurs in the above reaction mixture, and the hydrolysis product fatty acids then react with a weak base to form fatty acid salts, thereby achieving saponification; (3) Continue stirring for 30 minutes until the reaction mixture becomes a paste, then stop stirring and pour it into a mold; (4) After the soap body is fully cured, it is packaged.

2. The method for producing handmade soap using lipase catalysis according to claim 1, characterized in that: The temperature at which the grease is kept in a molten state is controlled between 10°C and 45°C.

3. The method for producing handmade soap using lipase catalysis according to claim 2, characterized in that: The oil can be animal fat, vegetable oil, or a mixture thereof.

4. The method for producing handmade soap using lipase catalysis according to claim 1, characterized in that: The weak base may be sodium carbonate, sodium bicarbonate, or a mixture thereof; the mass of the weak base added is calculated based on the saponification value of the oil used.

5. The method for producing handmade soap using lipase catalysis according to claim 1, characterized in that: The alkaline lipase is an enzyme preparation produced by microbial fermentation and having single alkaline lipase activity, or an enzyme preparation extracted from animal pancreas and having multiple enzyme activities, including alkaline lipase; or a mixture of the above two enzyme preparations.

6. The method for producing handmade soap using lipase catalysis according to claim 5, characterized in that: The concentration of the enzyme preparation in the aqueous solution is 1%–10%.

7. The method for producing handmade soap using lipase catalysis according to claim 6, characterized in that: The amount of the lipase-containing aqueous solution added is 4%–40% of the substrate mixture.

8. The method for producing handmade soap using lipase catalysis according to claim 1, characterized in that: The post-ripening time is 1-7 days.