Ficus pendula growth inhibitor and use method thereof

Abstract

The invention discloses a ficus sarmentosa growth inhibitor and a use method thereof, the effective components of the ficus sarmentosa growth inhibitor comprise 400-600mg / L paclobutrazol, 200mg / L uniconazole, 800mg / L mepiquat chloride, or 400mg / L paclobutrazol, 200mg / L uniconazole, and 3000mg / L. When the ficus sarmentosa growth inhibitor is used, the inhibitor is sprayed on all leaf surfaces of a plant until the plant is wet, and no watering and no rain exist within 24 hours after the inhibitor is applied. The growth inhibitor has the advantages that the growth inhibitor has a good inhibition effect on ficus sarmentosa, the inhibition rate of newly grown branches is 63.7%, the maintenance cost is reduced, the labor intensity of workers is relieved, plant leaf deformity can be reduced, and the ornamental effect of plants is guaranteed.

Description

Technical Field

[0001] This invention relates to the field of plant growth regulators, and more particularly to growth inhibitors. Background Technology

[0002] Weeping fig (Ficus benjamina L.) is a tree belonging to the genus Ficus in the family Moraceae. It occupies an irreplaceable and important position in the greening field in areas with abundant rainfall (such as South China and Southern Fujian), and its application scenarios are wide-ranging and its functions are significant.

[0003] In highway landscaping, weeping fig (Ficus benjamina) is an ideal supplementary greening species for the median strip in warm southern regions. By pruning and controlling its height and crown width to maintain a regular shape, it can be combined with other shrubs to form a continuous green belt. This effectively blocks glare from oncoming headlights, ensuring driving safety, and clearly separates two-way traffic. At the same time, its evergreen nature ensures the consistency of the green landscape throughout the year, avoiding the problem of reduced protective effect and landscape integrity caused by leaf fall.

[0004] In terms of hedge creation, the weeping fig (Ficus benjamina) stands out. Its branches are flexible and tolerant of pruning, and its shape is highly malleable. It can be pruned into various shapes such as neat rectangles, cylinders, or waves to meet different needs. When the height is controlled between 0.8 and 2 meters, it is suitable for various scenarios such as courtyards, parks, and road medians. At the same time, its leaves are small and dense (5-10 cm long) and evergreen all year round. After pruning, it can quickly sprout new branches to form a well-sealed green wall. It can not only effectively block the view and divide the space, but also has practical functions such as sound insulation and dust prevention, making it an excellent choice for improving the quality of the environment.

[0005] However, the maintenance of weeping figs also faces significant challenges. On the one hand, their rapid growth means that failure to prune them promptly in highway landscaping can impede traffic, and they require multiple prunings annually to maintain their shape when used for hedges. Relying solely on pruning to control their form requires a substantial investment of manpower and time, making practical operation extremely inconvenient. On the other hand, the application of existing growth inhibitors is not ideal. Because the leaves of weeping figs are covered with a waxy layer, ordinary liquid agents have difficulty penetrating them. Adding penetration enhancers such as organosilicon not only increases costs but may also damage the leaves. The effectiveness of chemical inhibitors is greatly affected by environmental factors such as temperature, humidity, and light, and since weeping figs can grow over 1 meter annually, frequent application is necessary, undoubtedly increasing labor costs. While bio-based inhibitors (such as plant extracts) have the advantage of being environmentally friendly, their active ingredients are easily affected by temperature and pH, resulting in large fluctuations in efficacy. They also need to be sprayed in specific seasons, and their effective period is only 2-3 months, which is far shorter than the effective period of chemical agents. In addition, extracting active ingredients (such as flavonoids) from weeping figs or other plants requires complex processes and consumes a large amount of raw materials, so the actual application cost may be far higher than that of chemical agents.

[0006] More importantly, long-term use of the same inhibitor may lead to resistance in weeping figs, requiring increased dosage to maintain the effect, posing a certain risk of resistance. At the same time, improper use of inhibitors can also negatively affect the appearance of weeping figs, potentially causing leaves to become smaller, discolored, or deformed, or even causing excessive shortening of internodes, thus destroying their original naturally drooping and beautiful ornamental form and reducing their landscape value in greening projects. Summary of the Invention

[0007] The purpose of this invention is to provide a growth inhibitor for weeping fig and its method of use, so as to solve the problem that there is no effective inhibitor in the prior art that can inhibit the growth of weeping fig without affecting its ornamental form.

[0008] To achieve the above objectives, the present invention adopts the following technical solution:

[0009] A growth inhibitor for weeping fig, the active ingredients of which include: 400-600 mg / L paclobutrazol, 200 mg / L uniconazole, 800 mg / L mepiquat chloride, or 400 mg / L paclobutrazol, 200 mg / L uniconazole, and 3000 mg / L butyrylhydrazine.

[0010] Furthermore, the ingredients also include adjuvants acceptable for pesticide preparation.

[0011] Furthermore, the additives include one or more of the following: wetting agents, dispersants, thickeners, defoamers, antifreeze agents, stabilizers, and water.

[0012] Furthermore, growth inhibitors can be formulated into emulsions, suspensions, suspensions, wettable powders, or water-dispersible granules.

[0013] The present invention also provides an application of the aforementioned weeping fig growth inhibitor as an inhibitor of weeping fig growth.

[0014] The present invention also provides a method for using the aforementioned weeping fig growth inhibitor: spray the entire leaf surface of the plant with the weeping fig growth inhibitor until it is moist, and do not water or allow rain within 24 hours after application.

[0015] The advantages of this invention are: the growth inhibitor has a good inhibitory effect on weeping fig, with a new shoot growth inhibition rate of 63.7%, reducing maintenance costs, reducing the labor intensity of workers, and also reducing plant leaf deformities, thus ensuring the ornamental effect of the plant. Attached Figure Description

[0016] The accompanying drawings, which are included to provide a further understanding of the invention and form part of this application, do not constitute an undue limitation of the invention. In the drawings:

[0017] Figure 1This is a statistical chart showing the effects of different reagents, concentrations, and application methods on the length of new shoots of Ficus weepingii.

[0018] Figure 2 This is a statistical chart showing the effects of different reagents, concentrations, and application methods on the number of leaves on new shoots of Ficus weepingii.

[0019] Figure 3 This is a statistical chart showing the effects of different reagents, concentrations, and application methods on the number of nodes in the new shoots of Ficus weepingii.

[0020] Figure 4 This is a statistical chart showing the effects of different reagents, concentrations, and application methods on the internode length of new shoots of Ficus weepingii.

[0021] Figure 5 This is a statistical chart showing the effects of different reagents, concentrations, and application methods on deformed leaves of the weeping fig.

[0022] Figure 6 This is a statistical chart showing the effects of different reagents, concentrations, and application methods on the number of branches on new shoots of Ficus weepingii.

[0023] Figure 7 This is a statistical chart showing the effects of different reagents, concentrations, and application methods on the SPAD value of Ficus weepingii leaves.

[0024] Figure 8 This is a statistical chart showing the effects of different reagents, concentrations, and application methods on the fresh weight of new shoots of Ficus weepingii.

[0025] Figure 9 This is a statistical graph showing the effects of different reagent combinations on the length of new shoots of Ficus weepingii.

[0026] Figure 10 This is a statistical chart showing the effects of different reagent combinations, concentrations, and application methods on the number of internodes in new shoots of Ficus weepingii.

[0027] Figure 11 This is a statistical chart showing the effects of different reagent combinations, concentrations, and application methods on deformed leaves of the weeping fig.

[0028] Figure 12 This is a statistical chart showing the effects of different reagent combinations, concentrations, and application methods on the number of branches on new shoots of Ficus weepingii. Detailed Implementation

[0029] The present invention will now be described in detail with reference to the accompanying drawings and specific embodiments. The illustrative embodiments and descriptions of the present invention are used to explain the present invention, but are not intended to limit the present invention.

[0030] 1. Effects of different reagent types, concentrations, and application methods on *Ficus benjamina*

[0031] Weeping fig trees with a crown width of 0.5-0.8m were selected as experimental subjects. The experimental agents used were paclobutrazol wettable powder (15% active ingredient) and chlormequat chloride (C5H). 13 C l2 N) and butyrylhydrazide (C6H11N2O3) were used as test reagents, with water, commercially available plant growth inhibitor product 1, and commercially available plant growth inhibitor product 2 as controls. Different concentrations of each reagent were set, as detailed in Table 1.

[0032] The experiment employed either foliar spraying or root application. For foliar spraying, the dosage was sufficient to moisten the entire leaf surface, with five pots per treatment. Seedling water and fertilizer management followed normal production procedures. Pruning was carried out one day prior to the experiment. After pruning, three branches from each plant were selected and marked for subsequent data measurement. The first application of the pesticide was given after pruning, followed by a second application 15 days later. No watering or rain was permitted within 24 hours of application. One month after stopping pesticide application, morphological and physiological / biochemical indicators of the plants were monitored, and the observation period lasted for three months.

[0033] Table 1. Different application methods and concentration settings for plant growth inhibitors.

[0034]

[0035] Commercially available plant growth inhibitor product 1 is a package product that includes a wettable powder containing 5% clopidogrel, an agent containing 30% polyazole·methiocarbamate, and a water-soluble fertilizer containing high phosphorus and potassium macroelements.

[0036] Commercially available plant growth inhibitor product 2 is a suspension containing 24% chlormequat chloride and 6% paclobutrazol.

[0037] index:

[0038] (1) Length of new shoots.

[0039] (2) Number of leaves on new shoots.

[0040] (3) Number of nodes in new shoots.

[0041] (4) Internode length of new shoots: Internode length = Branch length / Number of nodes.

[0042] (5) Number of deformed leaves: Record the number of deformed leaves on each plant that are twisted, yellowed, or dried.

[0043] (6) Number of branches of new shoots.

[0044] (7) Chlorophyll content: The SPAD value of mature leaves on new shoots is measured. This value represents the relative value of chlorophyll content in leaves and also represents the greenness of the plant. The SPAD value of mature leaves on marked branches is measured three months after the application of the pesticide using a portable SPAD-502 chlorophyll meter.

[0045] (8) Fresh weight of branches: Cut branches that have grown after the application of the medicine from the marked point and weigh them.

[0046] Data processing:

[0047] (1) Basic data processing and correlation analysis. The measured data were entered and preliminarily processed using WPS XLSL spreadsheets, and correlation analysis was performed using IBM SPSS Statistics 21 software.

[0048] (2) Principal component analysis. Correlation and principal component analysis were performed on the measurement data three months after drug application, and a comprehensive evaluation was conducted using principal component factors based on the principal component analysis.

[0049] Results and Analysis:

[0050] (1) Effects of plant growth inhibitors on the length of new shoots of Ficus weepingii

[0051] Depend on Figure 1 It was found that the length of new shoots of Ficus benjamina differed significantly three months after treatment (p<0.05). Among them, the new shoots with the root application of 1500 mg / L chlormequat chloride (BY3) were the longest, at 228.26 mm; while the shoots with the spray application of 1800 mg / L paclobutrazol (AX3) were the shortest, at 12.86 mm.

[0052] As shown in Table 1, compared with the water control, the dwarfing effects of different reagents on the branch length of *Ficus benjamina* varied significantly. Three months after application, the dwarfing rate of new branches treated with paclobutrazol ranged from 89.29% to 93.82%; that of chlormequat chloride ranged from 0.42% to 56.11%, with only the application of 1000 mg / L chlormequat chloride (BX1) resulting in a branch length dwarfing rate exceeding 50%; and that of butyrylhydrazine treated with butyrylhydrazine ranged from 4.67% to 60.55%, with only the application of 3000 and 4000 mg / L butyrylhydrazine (CX3) resulting in a branch length dwarfing rate exceeding 50%. The experimental results indicate that paclobutrazol's dwarfing effect on branch length is far superior to that of chlormequat chloride and butyrylhydrazine. Furthermore, the dwarfing rates of commercially available plant growth inhibitors 1 and 2 on new branches were 92.27% and 91.80%, respectively, which were lower than those achieved by spraying 1800 mg / L paclobutrazol (AX3).

[0053] Table 1. Inhibition rates of different treatments on various indicators of Ficus benjamina compared to the water control. Length of shoot Number of leaves Number of nodes Length of internode Branching SPAD value Fresh weight of shoot Number of abnormal leaves AX1 -89.59% -85.25% -48.55% -76.48% -80.28% 2.72% -91.37% 30.80 AX2 -91.74% -83.48% -59.42% -74.38% -80.28% -7.08% -91.49% 16.60 AX3 -93.82% -89.09% -69.57% -73.20% -80.28% 52.97% -95.86% 7.60 BX1 -56.11% -68.44% -38.41% -36.17% -63.18% 2.86% -56.81% 1.00 BX2 -18.37% -46.31% -14.49% -4.05% -35.57% -1.88% -40.21% 0.00 BX3 -29.28% -36.58% -21.74% -16.22% -23.73% 0.17% -46.42% 0.00 CX1 -49.35% -41.59% -26.81% -30.10% -42.14% -6.23% -50.96% 0.00 CX2 -60.55% -59.88% -35.51% -38.87% -53.98% -4.32% -70.72% 0.00 CX3 -52.04% -57.52% -30.43% -37.31% -59.24% -10.28% -61.43% 0.00 AY1 -91.83% -88.50% -62.32% -73.74% -80.28% -9.45% -94.70% 15.20 AY2 -90.86% -87.91% -62.32% -69.59% -80.28% -3.56% -92.97% 13.00 AY3 -89.29% -88.50% -62.32% -68.16% -80.28% 1.88% -92.95% 10.00 BY1 -25.07% -45.13% -16.67% -11.53% -23.73% -0.30% -50.23% 0.00 BY2 0.42% -14.16% -7.97% 3.82% -15.84% 2.78% 2.54% 0.00 BY3 9.77% -24.19% -3.62% 12.72% -1.38% -6.14% -16.12% 0.00 CY1 -40.49% -61.36% -23.19% -17.57% -52.66% -3.75% -58.01% 0.00 CY2 -4.67% -31.86% -3.62% -0.06% -22.42% -0.89% -34.43% 0.00 CY3 -8.56% -26.84% -2.90% 4.29% -1.38% -0.14% -19.92% 0.00 CK2 -92.27% -86.14% -69.57% -70.98% -77.65% -7.37% -93.11% 0.00 CK3 -91.80% -82.30% -54.35% -79.80% -80.28% -6.00% -91.76% 18.00

[0054] (2) Effects of plant growth inhibitors on the number of leaves on new shoots of Ficus weepingii

[0055] Depend on Figure 2It was found that three months after application, there were significant differences in the number of leaves on new shoots of *Ficus microcarpa* under different treatments (p<0.05). As shown in Table 1, compared with the water treatment, paclobutrazol inhibited the number of leaves on new shoots by 83.48%–89.09%, chlormequat chloride by 14.16%–68.44%, and butyrylhydrazine by 26.84%–61.36%. The inhibition rates of commercially available plant growth inhibitor product 1 and product 2 were 86.14% and 82.30%, respectively. Paclobutrazol, commercially available plant growth inhibitor product 1, and commercially available plant growth inhibitor product 2 showed significantly stronger inhibitory effects on the number of leaves on new shoots than chlormequat chloride and butyrylhydrazine, with the strongest effect observed when 1800 mg / L paclobutrazol (AX3, 89.09%) was sprayed.

[0056] (3) Effects of plant growth inhibitors on the number of nodes in new shoots of Ficus weepingii

[0057] Depend on Figure 3 It was found that three months after application, there were significant differences in the number of nodes of new shoots in different treatments of *Ficus benjamina* (p<0.05). As shown in Table 1, compared with the water treatment, paclobutrazol inhibited the number of nodes of new shoots in *Ficus benjamina* by 48.55%–69.57%, chlormequat chloride by 3.62%–38.41%, and butyryl hydrazide by 2.90%–35.51%. The inhibition rates of commercially available plant growth inhibitor product 1 and commercially available plant growth inhibitor product 2 were 69.57% and 54.35%, respectively. Among them, spraying with 1800 mg / L paclobutrazol (AX3) and commercially available plant growth inhibitor product 1 had the strongest inhibitory effect, with an inhibition rate of 69.57% and a number of nodes of 2.80. The worst effect was root application of 900 mg / L butyryl hydrazide (CY3), with an inhibition rate of 2.90% and a number of nodes of 9.2.

[0058] (4) Effects of plant growth inhibitors on the internode length of new shoots of Ficus weepingii

[0059] Depend on Figure 4 It was found that there were significant differences in the internode length of new shoots of *Ficus benjamina* under different treatments (p<0.05). As shown in Table 1, compared with the water control, three months after application, paclobutrazol inhibited the internode length of new shoots of *Ficus benjamina* by 68.16%–76.48%, chlormequat chloride by 12.72% (promoting)–36.17%, and butyrylhydrazine by 4.29% (promoting)–38.87%. The inhibition rates of commercially available plant growth inhibitor product 1 and commercially available plant growth inhibitor product 2 were 70.98% and 79.80%, respectively.

[0060] The root application of 1500 mg / L chlormequat chloride (BY3) and 900 mg / L butyrylhydrazine (CY3) had a poor inhibitory effect on the internode length of new shoots of Ficus weepingii, with the internode length even exceeding that of the water treatment by 12.72% and 4.29%, respectively, reaching 23.77 mm and 22.00 mm. The most effective inhibitory effect was achieved by commercially available plant growth inhibitor product 2 (CK3), with the smallest internode length at 4.26 mm.

[0061] In terms of reagent type, paclobutrazol showed significantly stronger inhibitory effects than chlormequat chloride and butyryl hydrazine. Furthermore, the internode length of *Ficus microcarpa* treated with paclobutrazol remained at a low level for three months, indicating a long-lasting inhibitory effect on branch internode length. Regarding application method, three months after application, foliar application of paclobutrazol showed slightly stronger inhibitory effects than root application, while foliar application of chlormequat chloride and butyryl hydrazine showed significantly stronger effects than root application.

[0062] (5) Effects of plant growth inhibitors on deformed leaves of Ficus benjamina

[0063] Depend on Figure 5 It was found that there were significant differences in the number of deformed leaves among different treatments of *Ficus droopingensis* (p<0.05). Three months after treatment, almost no deformed leaves were produced after spraying or root application of chlormequat chloride and butyrylhydrazine. However, the application of paclobutrazol, commercially available plant growth inhibitor product 1 (CK2), and commercially available plant growth inhibitor product 2 (CK3) resulted in a significant number of deformed leaves. Furthermore, no deformed leaves were produced in the first month, but the number increased sharply in the third month. Among these treatments, the application of 1000 mg / L paclobutrazol (AX1) resulted in the highest number of deformed leaves (30.8 leaves), followed by the application of commercially available plant growth inhibitor product 2 (18 leaves).

[0064] (6) Effects of plant growth inhibitors on the number of branches of new shoots of Ficus weepingii

[0065] Depend on Figure 6 It was found that there were significant differences in the number of new branches of Ficus microcarpa under different treatments (p<0.05). Spraying or applying paclobutrazol or a commercially available plant growth inhibitor product 2 had the strongest inhibitory effect on branching, with a new branching number of 1. The weakest inhibitory effects were observed with root application of 1500 mg / L chlormequat chloride (BY3) and root application of 900 mg / L butyrylhydrazine (CY3), which had the highest average branching number of 5.0.

[0066] (7) Effects of plant growth inhibitors on SPAD value of Ficus weepingii leaves

[0067] Depend on Figure 7 It was found that, among all treatments, only the application of 1800 mg / L paclobutrazol (AX3) for three months resulted in a SPAD value of 70.66 for the leaves of new shoots of Ficus benjamina, which was different from other treatments. The SPAD values ​​of the other treatments did not differ significantly (p<0.05).

[0068] (8) Effects of plant growth inhibitors on the fresh weight of new shoots of Ficus weepingii

[0069] Depend on Figure 8 It was found that there were significant differences in the fresh weight of new shoots of *Ficus microcarpa* under different treatments (p<0.05). As shown in Table 1, compared with the water treatment, after three months, the dwarfing rate of shoots treated with paclobutrazol ranged from 91.37% to 95.86%, while that with chlormequat chloride ranged from 2.54% (promoting) to 56.81%, and that with butyrylhydrazine ranged from 19.92% to 70.72%. The dwarfing rates of shoots treated with commercially available plant growth inhibitors 1 and 2 were 93.11% and 91.76%, respectively. The strongest inhibitory effect was achieved by spraying with 1800 mg / L paclobutrazol (AX3), with a shoot fresh weight of 0.31 g. The weakest inhibitory effect was achieved by root application of 1200 mg / L chlormequat chloride (BY2), with a shoot fresh weight of 7.64 g. Paclobutrazol and other existing commercially available products were significantly more effective than chlormequat chloride and butyrylhydrazine.

[0070] Correlation analysis:

[0071] Table 2 shows that three months after application, there were no significant relationships between SPAD values ​​and any of the other indicators, or between the number of deformed leaves and the number of branches. However, highly significant relationships (p<0.01) were observed among the remaining indicators. Except for SPAD values, the other indicators played different roles in evaluating the effects of different treatments on the growth inhibition of *Ficus benjamina*. Therefore, further analysis combining all indicators is necessary.

[0072] Table 2. Correlation coefficients of various indicators of Ficus microcarpa 3 months after application of the drug. Index Length of shoot Number of leaves Number of nodes Length of internode Branching SPAD value Fresh weight of shoot Number of abnormal leaves Length of shoot 1 Number of leaves .957** 1 Number of nodes .977** .948** 1 Length of internode .990** .932** .968** 1 Branching .971** .969** .950** .956** 1 SPAD value -0.149 -0.153 -0.217 -0.147 -0.126 1 Fresh weight of shoot .968** .978** .944** .948** .953** -0.131 1 Number of abnormal leaves -.737** -.701** -.680** -.798** -.705** 0.028 -.700** 1

[0073] Comprehensive evaluation of the medicinal efficacy of Ficus benjamina based on principal component analysis:

[0074] As shown in Table 3, the values ​​measured three months after the application of the drug were processed through data standardization. Principal component analysis was performed on the various indicators under different treatments, and one principal component was extracted with a contribution rate of 80.078%.

[0075] Table 3. Principal component analysis of various indicators of Ficus microcarpa 3 months after application of the drug.

[0076] Based on the standard values ​​of the seven indicators and the eigenvectors of the factor score coefficients of the component matrix, the regression equation for the first principal component can be derived as follows:

[0077] C = 0.991*X1 + 0.975*X2 + 0.974*X3 + 0.988*X4+ 0.977*X5 - 0.171*X6 +0.975*X7 - 0.784*X8,

[0078] The comprehensive scores and rankings of each plant were obtained through calculation, as shown in Table 4. The order of plant growth inhibition efficacy of different reagents, concentrations, and application methods is as follows: 1000 mg / L paclobutrazol spray > 1800 mg / L paclobutrazol spray > spraying commercially available plant growth inhibitor product 2 > 400 mg / L paclobutrazol root application > 1400 mg / L paclobutrazol spray > 600 mg / L paclobutrazol root application > spraying commercially available plant growth inhibitor product 1 > root application of 800 mg / L paclobutrazol > spraying of 3000 mg / L butyrylhydrazine > spraying of 1000 mg / L chlormequat chloride > spraying of 4000 mg / L butyrylhydrazine > spraying of 2000 mg / L butyrylhydrazine > root application of 500 mg / L butyrylhydrazine > spraying of 1800 mg / L chlormequat chloride > root application of 900 mg / L chlormequat chloride > spraying of 1400 mg / L chlormequat chloride > root application of 900 mg / L butyrylhydrazine > Root application of 700 mg / L butyrylhydrazine > Root application of 1200 mg / L chlormequat chloride > Spraying with water > Root application of 1500 mg / L chlormequat chloride.

[0079] Table 4. Overall evaluation of the effects of different treatments on inhibiting the growth of Ficus benjamina. Treatment number Treatment Score (C) Rank AX1 Spraying 1000 mg / L of paclobutrazol 3.56 1 AX2 Spraying 1400 mg / L of paclobutrazol 10.88 5 AX3 Spraying 1800 mg / L of paclobutrazol 6.70 2 BX1 Spraying 1000 mg / L of mepiquat chloride 112.27 10 BX2 Spraying 1400 mg / L of mepiquat chloride 207.48 16 BX3 Spraying 1800 mg / L of mepiquat chloride 183.94 14 CX1 Spraying 2000 mg / L of butyraldehyde hydrazide 137.39 12 CX2 Spraying 3000 mg / L of butyraldehyde hydrazide 105.50 9 CX3 Spraying 4000 mg / L of butyraldehyde hydrazide 125.22 11 AY1 Rooting 400 mg / L of paclobutrazol 10.51 4 AY2 Rooting 600 mg / L of paclobutrazol 14.90 6 AY3 Rooting 800 mg / L of paclobutrazol 20.23 8 BY1 Rooting 900 mg / L of mepiquat chloride 191.91 15 BY2 Rooting 1200 mg / L of mepiquat chloride 259.24 19 BY3 Rooting 1500 mg / L of mepiquat chloride 278.60 21 CY1 Rooting 500 mg / L of butyraldehyde hydrazide 153.00 13 CY2 Rooting 700 mg / L of butyraldehyde hydrazide 241.70 18 CY3 Rooting 900 mg / L of butyraldehyde hydrazide 237.79 17 CK1 Spraying water 262.22 20 CK2 Spraying commercially available plant growth inhibitor product 1 16.40 7 CK3 Spraying commercially available plant growth inhibitor product 2 9.14 3

[0080] Effects of different treatments on growth inhibition of Ficus benjamina:

[0081] The test results are shown in Table 4. Compared with similar products, the scores of spraying 1000 mg / L paclobutrazol (AX1) and spraying 1800 mg / L paclobutrazol (AX3) were lower than those of commercially available plant growth inhibitor product 1 (CK2) and commercially available plant growth inhibitor product 2 (CK3), indicating that the inhibitory effect was stronger than that of similar products.

[0082] As shown in Table 5, among the three reagents paclobutrazol, chlormequat chloride, and butyrylhydrazine, paclobutrazol showed the best inhibitory effect on various indicators of weeping fig. The comprehensive evaluation results by principal component analysis showed that spraying 1000 mg / L paclobutrazol (AX1) had the best inhibitory effect.

[0083] Table 5. Comparison of the effects of different treatments on the growth inhibition of Ficus benjamina. Comparison among three reagents Comparison among application methods of reagent with strongest inhibitory effect Concentration with strongest inhibitory effect Comparison with commercially available plant growth inhibitor product 1 CK2 and commercially available plant growth inhibitor product 2 CK3 Length of shoot Paclobutrazol has the strongest inhibitory effect No significant difference Spraying 1800 mg / L of paclobutrazol (AX3) The difference is not significant, branch length: AX3 < CK2 < CK3 Number of leaves Paclobutrazol has the strongest inhibitory effect No significant difference Spraying 1800 mg / L of paclobutrazol (AX3) The difference is not significant, the number of leaves: AX3 < CK2 < CK3 Number of nodes Paclobutrazol has the strongest inhibitory effect Rooting has slightly stronger effect than spraying Spraying 1800 mg / L of paclobutrazol (AX3) The difference is not significant, the number of nodes: AX3 = CK2 < CK3 Length of internode Paclobutrazol has the strongest inhibitory effect No significant difference Spraying 1000 mg / L of paclobutrazol (AX1) The difference is not significant, the length of the internode: CK3 < AX1 < CK2 Number of abnormal leaves Paclobutrazol has the most abnormal leaves Spraying is more than rooting Spraying 1000 mg / L of paclobutrazol (AX1) Significant difference, number of abnormal leaves: AX1>CK3>CK2 Number of branches Paclobutrazol has the strongest inhibitory effect No difference No new branches of new shoots are generated by paclobutrazol Significant difference, branch number: paclobutrazol = CK3 < CK2 Leaf SPAD value No significant difference No significant difference Spraying 1800 mg / L of paclobutrazol (AX3) is the largest No significant difference, SPAD value: AX3>CK3>CK2 Fresh weight of shoot Paclobutrazol has the strongest inhibitory effect No significant difference Spraying 1800 mg / L of paclobutrazol (AX3) No significant difference, fresh weight of shoot: CK3>CK2>AX3

[0084] In subsequent compound agent trials, it may be considered to add reagents that can reduce leaf deformities and adjust the concentration of paclobutrazol to ensure the landscape effect of the green belt while inhibiting plant growth.

[0085] 2. Effects of different compound formulations on Ficus benjamina

[0086] Weeping fig trees with a crown width of 0.5–0.8 m were selected as the experimental subjects. Seven plant growth inhibitors from four categories—carbon-containing heterocyclic compounds, ononium compounds, acylcyclohexane derivatives, and growth inhibitors—were chosen as test reagents, with water serving as a control. Different concentrations of each reagent were set, as detailed in Table 6.

[0087] The experiment adopted a spraying method, with the amount sprayed enough to wet all leaf surfaces. Five pots were used for each treatment. Water and fertilizer management of the seedlings were carried out according to normal production procedures. Pruning was carried out one day in advance. After pruning, three branches from each plant were selected and marked for subsequent data measurement. The pesticide was applied once after pruning. No watering or rain was allowed within 24 hours after application. Morphological and physiological-biochemical indicators of the plants were monitored and measured every two months, with a follow-up observation period of three months.

[0088] Table 6. Setting of Plant Growth Inhibitors for Spraying Weeping Fig

[0089] index:

[0090] (1) Length of new shoots.

[0091] (2) Number of internodes: The number of internodes of newly sprouted branches.

[0092] (3) Internode length: Based on the number of nodes on the branch, the internode length = branch length / number of nodes.

[0093] (4) Number of deformed leaves: Record the number of deformed leaves on each plant that are twisted, yellowed, or dried.

[0094] (5) Number of branches: The number of branches of newly sprouted branches.

[0095] Data processing:

[0096] (1) Basic data processing and correlation analysis. The measured data were entered and preliminarily processed using WPS XLSL spreadsheets, and correlation analysis was performed using IBM SPSS Statistics 21 software.

[0097] (2) Membership function analysis. All experimental data were initially processed using Excel 2003 software. Statistical analysis was performed using SAS 8.0 software. The membership function method was used to comprehensively analyze 14 leaf anatomical structure indicators; the calculation formula is: U i =(Ui -U min ) / (U max -U min ), where U i U is the measured value of the index. min U max These represent the minimum and maximum values ​​of a certain index for all experimental materials. If a certain index is negatively correlated with the heat resistance of a variety, then the inverse membership function is used for conversion, and the calculation formula is: U i =1-(U i -U min ) / (U max -U min ).

[0098] Experimental Results and Analysis:

[0099] (1) Effects of plant growth inhibitors on the length of new shoots of Ficus weepingii

[0100] Depend on Figure 9 It was found that the length of new shoots of *Ficus benjamina* after three months varied significantly under different treatments (p<0.05). As shown in Table 7, the shortest new shoot length (31.61 mm) was achieved with spraying 400 mg / L paclobutrazol + 25 mg / L abscisic acid + 100 mg / L triiodobenzoic acid, with an inhibition rate of 64.17%. The second shortest was achieved with 400 mg / L paclobutrazol + 200 mg / L uniconazole + 800 mg / L mepiquat chloride, with a length of 32.02 mm and an inhibition rate of 63.7%.

[0101] Table 7. Inhibition rate of different treatments on various indicators of Ficus benjamina six months after spraying inhibitor. Serial number Average length of shoot Average number of internodes of shoot Average length of internode of shoot Inhibitory rate of length of shoot Inhibitory rate of length of internode Number of branches Number of abnormal leaves 1 57.23 7.07 8.10 35.12% 15.54% -14.29% 15.09% 2 32.02 6.27 5.11 63.70% 46.72% -80.95% -2.83% 3 59.35 5.87 10.12 32.72% -5.50% 0.00% -6.60% 4 40.08 5.93 6.75 54.57% 29.56% 14.29% 5.66% 5 47.12 5.93 7.94 46.59% 17.19% 0.00% -13.21% 6 33.00 6.13 5.38 62.59% 43.89% 19.05% 10.38% 7 65.16 6.27 10.40 26.14% -8.43% -76.19% -12.26% 8 83.77 6.93 12.08 5.05% -26.00% 19.05% -1.89% 9 53.16 6.67 7.97 39.74% 16.84% 23.81% -7.55% 10 31.61 5.20 6.08 64.17% 36.61% 28.57% 0.94% 11 41.16 5.73 7.18 53.34% 25.12% 23.81% -14.15% 12 44.87 6.07 7.40 49.13% 22.86% 9.52% -1.89% 13 64.46 7.00 9.21 26.93% 3.97% 14.29% 9.43% 14 71.30 6.73 10.59 19.18% -10.43% 23.81% -15.09% CK 88.22 9.20 9.59 0.00%

[0102] (2) Effects of plant growth inhibitors on the number and length of internodes of new shoots of Ficus weepingii

[0103] The more nodes a newly sprouted branch of a weeping fig has, the more new leaves it produces, resulting in a better visual effect. Shorter internodes, on the other hand, have a stronger suppressive effect. Figure 10 It can be seen that the treatment with the highest average number of internodes in newly sprouted branches was the control treatment, i.e., spraying with water. All combinations of inhibitors had a certain inhibitory effect on the number of internodes in *Ficus benjamina*. As shown in Table 7, the treatment with the shortest internode length was 400 mg / L paclobutrazol + 200 mg / L uniconazole + 800 mg / L mepiquat chloride, with an inhibition rate of 46.72%.

[0104] (3) Effects of plant growth inhibitors on deformed leaves of Ficus benjamina

[0105] Depend on Figure 11It can be seen that after spraying the inhibitor for 2 months, the difference in deformed leaves of Ficus benjamina was significant. The longer the spraying time, the more the number of deformed leaves decreased. After spraying the inhibitor for 6 months, the treatment with the fewest deformed leaves was 600 mg / L paclobutrazol + 200 mg / L uniconazole + 800 mg / L mepiquat chloride.

[0106] (4) Effects of plant growth inhibitors on the number of branches of new shoots of Ficus weepingii

[0107] Depend on Figure 12 It can be seen that spraying with 400 mg / L paclobutrazol + 200 mg / L uniconazole + 800 mg / L chlormequat chloride or 600 mg / L paclobutrazol + 1000 mg / L chlormequat chloride + 3000 mg / L butyrylhydrazine for 6 months resulted in more branches in the weeping fig, while the other treatments showed no significant difference.

[0108] Comprehensive evaluation of the medicinal efficacy of Ficus benjamina based on membership function analysis:

[0109] The membership function method was used to comprehensively analyze the growth indicators, as shown in Table 8. According to the average membership values ​​of each indicator, the inhibitory effects of each treatment from strongest to weakest are as follows: 2 > 6 > 1 > 4 > 10 > 12 > 13 > CK > 7 > 9 > 5 > 11 > 3 > 8 > 14. That is, the treatment with the strongest comprehensive inhibitory effect is 400 mg / L paclobutrazol + 200 mg / L uniconazole + 800 mg / L mepiquat chloride.

[0110] Table 8. Membership function values ​​of various growth indicators of *Ficus microcarpa* 6 months after spraying inhibitor. Serial Number Average branch length Average number of internodes per branch Average internode length of branches Number of branches Number of abnormal leaves Average membership value Sort 1 0.55 0.47 0.57 0.39 1.00 0.60 3 2 0.99 0.27 1.00 1.00 0.41 0.73 1 3 0.51 0.17 0.28 0.26 0.28 0.30 13 4 0.85 0.18 0.76 0.13 0.69 0.52 4 5 0.73 0.18 0.59 0.26 0.06 0.37 11 6 0.98 0.23 0.96 0.09 0.84 0.62 2 7 0.41 0.27 0.24 0.96 0.09 0.39 9 8 0.08 0.43 0.00 0.09 0.44 0.21 14 9 0.62 0.37 0.59 0.04 0.25 0.37 10 10 1.00 0.00 0.86 0.00 0.53 0.48 5 11 0.83 0.13 0.70 0.04 0.03 0.35 12 12 0.77 0.22 0.67 0.17 0.44 0.45 6 13 0.42 0.45 0.41 0.13 0.81 0.44 7 14 0.30 0.38 0.21 0.04 0.00 0.19 15 CK 0.00 1.00 0.36 0.26 0.50 0.42 8

[0111] The technical solutions provided by the embodiments of the present invention have been described in detail above. Specific examples have been used to illustrate the principles and implementation methods of the embodiments of the present invention. The descriptions of the embodiments above are only for helping to understand the principles of the embodiments of the present invention. At the same time, for those skilled in the art, there will be changes in the specific implementation methods and application scope based on the embodiments of the present invention.

Claims

1. A growth inhibitor for weeping fig, characterized in that, Its active ingredients include: 400~600mg / L paclobutrazol, 200mg / L uniconazole, 800mg / L mepiquat chloride, or 400mg / L paclobutrazol, 200mg / L uniconazole, and 3000mg / L butyrylhydrazine.

2. The growth inhibitor for weeping fig according to claim 1, characterized in that, The ingredients also include adjuvants acceptable for pesticide preparation.

3. The weeping fig growth inhibitor according to claim 2, characterized in that, Additives include one or more of the following: wetting agents, dispersants, thickeners, defoamers, antifreeze agents, stabilizers, and water.

4. The growth inhibitor for weeping fig according to claim 2, characterized in that, Growth inhibitors can be formulated into emulsions, suspensions, suspensions, wettable powders, or water-dispersible granules.

5. The use of a *Ficus benjamina* growth inhibitor as described in any one of claims 1-4 as an inhibitor of *Ficus benjamina* growth.

6. A method of using the *Ficus benjamina* growth inhibitor as described in any one of claims 1-4, characterized in that: Spray all leaf surfaces of the plant with a growth inhibitor for weeping fig until moist. Do not water or allow rain within 24 hours after application.

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