Rubber tree bark beetle attractant and lure preparation and application thereof

Abstract

The invention relates to the technical field of rubber tree bark beetle prevention and control and green ecological prevention and control, in particular to a rubber tree bark beetle attractant and lure preparation and application thereof. Comprising the following components in parts by weight: 1 part of (S)-cis-verbenol, 1 part of isoamyl alcohol, 1 part of longifolene and 6500 parts of absolute ethyl alcohol. The attractant can attract bark beetles on rubber trees, has the advantages of being good in attraction effect, long in lasting period, environmentally friendly and the like and is suitable for long-term monitoring and efficient prevention and control of bark beetle populations on the rubber trees, and the attraction core is not affected by terrains and terrains when applied.

Description

Technical Field

[0001] This invention relates to the field of rubber tree bark beetle control and its green ecological control technology, and in particular to a rubber tree bark beetle attractant, lure preparation and application. Background Technology

[0002] Bark beetles are a collective term for bark beetles of the order Coleoptera that bore into the trunks of rubber trees. It has been reported that there are over 40 species of bark beetles that damage rubber trees, primarily belonging to the families Platypadidae and Scolytidae. These beetles mainly infest wind-fallen, wind-broken, weakened (due to natural disasters or mechanical damage), and dying trees in rubber plantations. In high-density populations, they can also directly infest healthy, standing trees, causing severe borer damage to the tapped surface and the area within 50cm above and below it, leading to latex exudation.

[0003] Currently, in rubber plantations, controlling bark beetles through physical and chemical methods is a safe and feasible approach. This involves using highly effective attractants in conjunction with bark beetle traps. Attracted by the attractant, the bark beetles fly to the trap, crash into it, and fall into the collection bottle, achieving a killing effect. However, unlike bark beetles which bore into the xylem, bark beetles that damage rubber tree trunks have different characteristics. For these bark beetles, no specific aggregation pheromone has yet been identified, and ethanol is commonly used as an attractant. Existing studies have evaluated the attraction effects of other bark beetle aggregation pheromones on rubber tree bark beetles, confirming that (S)-cis-verbenol has a good attraction effect. However, high-gradient concentration screening tests have not been conducted, so the appropriate dosage for field application remains unknown.

[0004] In fact, during the invasion and damage of rubber tree bark beetles, their invasion behavior is influenced not only by the aggregation pheromones released by the beetles themselves, but also by the crucial host-locating chemical signals from rubber tree volatiles. The bark beetles select and attack their host plants by recognizing these signals, ultimately establishing colonies. Existing reports indicate that phytochemicals can modulate the attraction effect of bark beetle aggregation pheromones, exhibiting either enhancement or weakening of the attraction. Furthermore, compared to bark beetle aggregation pheromones, plant volatiles offer advantages such as lower cost, greater variety, and environmental friendliness. Therefore, by combining plant volatiles with (S)-cis-verbenol, a known effective attractant for rubber tree bark beetles, and screening for synergistic chemicals, the combined use of plant volatiles and (S)-cis-verbenol can enhance the attraction effect on rubber tree bark beetles, thereby achieving highly efficient ecological control of this pest. Summary of the Invention

[0005] To address the aforementioned problems, this invention provides an attractant for rubber tree bark beetles, preparation of the lure core, and its application. The attractant provided by this invention can achieve highly efficient attraction of rubber tree bark beetles.

[0006] To achieve the above objectives, the present invention provides the following technical solution:

[0007] This invention provides an attractant for rubber tree bark beetles, comprising the following components in parts by weight:

[0008] 1 part (S)-cis-verbenol, 1 part isoamyl alcohol, 1 part longleafene and 6500 parts anhydrous ethanol.

[0009] The present invention also provides the application of the attractant described in the above technical solution in attracting bark beetles of rubber trees.

[0010] The present invention also provides a lure for attracting bark beetles on rubber trees, comprising a slow-release carrier, absorbent cotton placed in the slow-release carrier, and the attractant described in the above-mentioned technical solution soaked in the absorbent cotton.

[0011] Preferably, in a single lure core: the content of (S)-cis-verbenol is 3 mg; the content of isoamyl alcohol is 3 mg; the content of longleafene is 3 mg; and the content of anhydrous ethanol is 19.5 g.

[0012] Preferably, the slow-release carrier comprises a polyethylene plastic bag.

[0013] Preferably, the polyethylene plastic bag has the following specifications: 10 mils, black, 120 mm in length, and 80 mm in width.

[0014] Preferably, the content of degreased cotton in a single core is 5g.

[0015] The present invention also provides a method for preparing the lure core described in the above technical solution, comprising the following steps:

[0016] The absorbent cotton is placed in a slow-release carrier, and the attractant is then dripped onto the absorbent cotton. After sealing, the attractant core is obtained.

[0017] The present invention also provides the application of the lure described in the above technical solution in attracting bark beetles of rubber trees.

[0018] Preferably, the application includes: using the lure core in conjunction with a cross-shaped bark beetle trap, setting it at the edge of the rubber forest or in an open area within the forest, with a spacing of 50m and a height of 1.5 to 2m above the ground.

[0019] The beneficial effects of this invention are:

[0020] The attractant provided by this invention can attract rubber tree bark beetles and has the advantages of good attraction effect, long-lasting effect and environmental friendliness. It is suitable for long-term monitoring and efficient control of rubber tree bark beetle populations, and the application of the lure is not affected by terrain or topography. Attached Figure Description

[0021] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the accompanying drawings used in the embodiments will be briefly described below.

[0022] Figure 1 The results of the experiment evaluating the bioactivity of (S)-cis-verbenol dosage in the attractant on the bark beetle of rubber tree;

[0023] Figure 2 The results of the bioactivity evaluation test of different components of the attractant on the rubber tree bark beetle;

[0024] Figure 3 The results of the experiment evaluating the bioactivity of rubber tree bark beetles on the effect of varying isoamyl alcohol dosage in the attractant;

[0025] Figure 4 The results of the experiment evaluating the bioactivity of rubber tree bark beetles on the effect of changes in the dosage of longifole in the attractant;

[0026] Figure 5 The results of the experiment evaluating the bioactivity of isoamyl alcohol in combination with longleafene and (S)-cis-verbenol in attractants against bark beetles on rubber trees.

[0027] Figure 6 The results of the experiment evaluating the duration of bioactivity of the attractant on the bark beetle of rubber trees. Detailed Implementation

[0028] This invention provides an attractant for the rubber tree bark beetle, comprising the following components in parts by weight: 1 part (S)-cis-verbenol, 1 part isoamyl alcohol, 1 part longifolene, and 6500 parts anhydrous ethanol. This invention does not have specific limitations on the source of the above reagents; commercially available products are acceptable. In this invention, the (S)-cis-verbenol (Sigma-Aldrich, purity >95%), isoamyl alcohol (Luon, purity >99%), longifolene (Xienen, purity >95%), and anhydrous ethanol (Xilong Scientific, purity >99.7%) are used.

[0029] The present invention also provides the application of the attractant described in the above technical solution in attracting bark beetles of rubber trees.

[0030] This invention also provides a lure for attracting bark beetles on rubber trees, comprising a slow-release carrier, absorbent cotton placed within the slow-release carrier, and the attractant described in the above-mentioned technical solution impregnated on the absorbent cotton. In this invention, the content of (S)-cis-verbenol in a single lure is preferably 3 mg; the content of isoamyl alcohol is preferably 3 mg; the content of longifolene is preferably 3 mg; and the content of anhydrous ethanol is preferably 19.5 g. In this invention, the slow-release carrier preferably comprises a polyethylene plastic bag. In this invention, the specifications of the polyethylene plastic bag are preferably: 10 mils, black, 120 mm in length, and 80 mm in width. In this invention, the content of absorbent cotton in a single lure is preferably 5 g. This invention does not have special requirements regarding the source of the absorbent cotton and the polyethylene plastic bag; commercially available products are acceptable.

[0031] The present invention also provides a method for preparing the lure core described in the above technical solution, comprising the following steps:

[0032] The absorbent cotton is placed in a slow-release carrier, and the attractant is then dripped onto the absorbent cotton. After sealing, the attractant core is obtained.

[0033] The present invention also provides the application of the lure described in the above technical solution in attracting bark beetles on rubber trees. In the present invention, the application preferably includes: using the lure in conjunction with a cross-shaped bark beetle trap, setting it at the edge of the rubber forest or in an open area within the forest, with a spacing of 50m and a height of 1.5 to 2m above the ground.

[0034] The rubber tree bark beetle lure provided by this invention can be used for large-scale trapping and killing of rubber tree bark beetles, or for long-term monitoring of rubber tree bark beetle populations. The lure is easy to process and produce, inexpensive, and its effectiveness lasts for about one month, meeting the needs of large-scale trapping of rubber tree bark beetles in production. This invention provides a highly efficient, long-lasting, and environmentally friendly lure for rubber tree bark beetles, demonstrating significant effectiveness in large-scale trapping and control. The lure has broad prospects for industrialization. Furthermore, its preparation method is simple, making it particularly suitable for large-scale promotion in production.

[0035] This invention also provides the application of the lure described in the above-mentioned technical solution in attracting bark beetles on rubber trees. In this invention, the lure is used in conjunction with a cross-shaped bark beetle trap, designed by Zhou Xiang et al., with design patent application number CN201430278516.3. Two plates are crossed to form a cross shape (380mm high, 230mm wide), secured to the top cover by a top clip, and connected to the funnel by a bottom clip. The insect collection bottle is directly connected to the funnel's flared opening below the funnel. The lure is suspended below the top of the trap at the intersection of the cross plates. The trap is suspended in the forest at a height of 1.5–2.0m above the ground, with a spacing of approximately 50m.

[0036] To further illustrate the present invention, the following detailed description is provided in conjunction with embodiments, but these should not be construed as limiting the scope of protection of the present invention.

[0037] Example 1

[0038] Evaluation results of the experiment on the bioactivity of (S)-cis-verbenol dosage in attractants on rubber tree bark beetles.

[0039] The rubber tree bark beetle attractant prepared in this embodiment uses commercially available (S)-cis-verbenol and anhydrous ethanol. The chemical purity of (S)-cis-verbenol is greater than 95%; the chemical purity of anhydrous ethanol is greater than 99.79%.

[0040] The following four treatments are prepared according to the testing requirements.

[0041] Treatment 1: 19.5 g anhydrous ethanol, 0.3 mg (S)-cis-verbenol;

[0042] Treatment 2: 19.5 g of anhydrous ethanol, 3 mg of (S)-cis-verbenol;

[0043] Treatment 3: 19.5 g of anhydrous ethanol, 30 mg of (S)-cis-verbenol;

[0044] Treatment 4: 19.5g of anhydrous ethanol and 300g of (S)-cis-verbenol.

[0045] The application example shows the preparation of a lure for attracting rubber tree bark beetles. The specific steps are as follows:

[0046] (1) Add anhydrous ethanol and (S)-cis-verbenol to the degreased cotton inside the polyethylene plastic belt according to the above usage amounts.

[0047] (2) Preparation and preservation of lure core: Add the lure to 5g of degreased cotton inside a polyethylene plastic bottle carrier, then place it in a black polyethylene plastic bag and seal it, and store it at 4℃.

[0048] (3) Field application of attractants:

[0049] a. Experimental location: Team 7, Natural Rubber Experimental Farm, Danzhou City, Hainan Province.

[0050] b. Construction of the trap: The lure is used in conjunction with a cross-shaped bark beetle trap designed by Zhou Xiang et al., with design patent application number CN201430278516.3. Two plates are crossed to form a cross shape (380mm high, 230mm wide), secured to the top cover via a hook at the top, and connected to the funnel via a hook at the bottom. The insect collection bottle is directly connected to the funnel's flared opening below the funnel.

[0051] c. Suspension of the lure: One lure is suspended at the junction of the cross plates below the top cover of the cross-shaped bark beetle trap.

[0052] d. Field setup of traps: Three replicates were set up for each experimental treatment. The traps were placed in an open area within the rubber plantation, with a spacing of approximately 50 meters between traps. The traps were fixed to the trunks of the rubber trees with wire, suspended at a height of 1.5–2.0 meters above the ground.

[0053] e. Data collection and trap emptying: Each treatment in this experiment was repeated three times. The number of bark beetles captured in each treatment was the average of the three traps. Depending on the weather conditions, the number of bark beetles in the collection bottle was observed every 5 to 7 days. After counting, the collected bark beetles were placed in disposable plastic resealable bags and brought back to the laboratory.

[0054] The above-described application steps are for attracting bark beetles on rubber trees. A field trapping experiment was conducted from January to February 2024; the specific results are detailed below. Figure 1 In this experiment, each treatment was replicated three times. The number of rubber tree bark beetles captured in each treatment was the average of the three traps. Depending on the weather conditions, the number of rubber tree bark beetles in the collection bottle was observed every 5 to 7 days.

[0055] from Figure 1 It can be seen that treatments 1 through 4 all attracted bark beetles of rubber trees, with treatment 2 capturing the largest number of beetles, significantly higher than treatment 1. While treatments 3 and 4 showed no significant difference in trapping effect compared to treatment 2, their trapping effects were significantly lower. These results indicate that the lure prepared using anhydrous ethanol in combination with 3 mg of (S)-cis-verbenol has a good attraction effect on bark beetles of rubber trees.

[0056] Example 2

[0057] Results of experiments evaluating the bioactivity of attractants with different components on bark beetles of rubber trees.

[0058] In Example 1, anhydrous ethanol was used as the solvent, and the optimal dosage of (S)-cis-verbenol for attracting animals in the field was 3 mg. Using anhydrous ethanol as the solvent, 3 mg of (S)-cis-verbenol was mixed with plant volatile components isoamyl alcohol, n-hexanol, longleafene, and 6-methyl-5-heptyl-2-ol to screen for chemicals that could enhance the attractant effect of (S)-cis-verbenol.

[0059] The rubber tree bark beetle attractant prepared in this embodiment uses commercially available (S)-cis-verbenol, isoamyl alcohol, n-hexanol, longifolene, 6-methyl-5-heptyl-2-ol, and anhydrous ethanol. The chemical purity of (S)-cis-verbenol is greater than 95%; isoamyl alcohol is greater than 99%; n-hexanol is greater than 99%; 6-methyl-5-heptyl-2-ol is greater than 95%; and anhydrous ethanol is greater than 99.79%.

[0060] The following six processes are configured according to the testing requirements.

[0061] Treatment 1: 19.5 g anhydrous ethanol, 3 mg (S)-cis-verbenol, 30 mg geraniol;

[0062] Treatment 2: 19.5g anhydrous ethanol, 3mg (S)-cis-verbenol, 30mg isoamyl alcohol;

[0063] Treatment 3: 19.5g anhydrous ethanol, 3mg (S)-cis-verbenol, 30mg n-hexanol;

[0064] Treatment 4: 19.5 g anhydrous ethanol, 3 mg (S)-cis-verbenol, 30 mg longleaf ethanoylene;

[0065] Treatment 5: 19.5g anhydrous ethanol, 3mg (S)-cis-verbenol, 30mg 6-methyl-5-hepta-2-ol;

[0066] Treatment 6: 19.5 g of anhydrous ethanol and 3 mg of (S)-cis-verbenol.

[0067] The lure cores for the rubber tree bark beetle prepared in this application example are prepared using the same method as in Example 1, including the same preparation method, preservation method, and implementation steps.

[0068] Following the specific implementation steps in Example 1, a field trapping experiment was conducted from March to April 2024. Specific results can be found in [link to example]. Figure 2 In this experiment, each treatment was replicated three times. The number of rubber tree bark beetles captured in each treatment was the average of the three traps. Depending on the weather conditions, the number of rubber tree bark beetles in the collection bottle was observed every 5 to 7 days.

[0069] from Figure 2 It can be seen that treatments 1 through 6 all attracted rubber tree bark beetles. Only treatment 1, with the combined use of geraniol and (S)-cis-verbenol, showed a significantly lower attraction effect than treatment 6, which used (S)-cis-verbenol alone. The other treatments attracted significantly more rubber tree bark beetles than treatment 6. Among these, treatment 2, with the combined use of isoamyl alcohol and (S)-cis-verbenol, showed the best attraction effect on rubber tree bark beetles, followed by the combination of longifolene and (S)-cis-verbenol. When (S)-cis-verbenol and anhydrous ethanol were used in combination with isoamyl alcohol or longifolene, the number of rubber tree bark beetles attracted increased significantly. This result indicates that isoamyl alcohol and longifolene can enhance the attraction effect of (S)-cis-verbenol and anhydrous ethanol on rubber tree bark beetles.

[0070] Example 3

[0071] Results of an experiment evaluating the bioactivity of rubber tree bark beetles on changes in the dosage of isoamyl alcohol in attractants.

[0072] In Example 2, isoamyl alcohol was found to have a significant synergistic effect on the attraction of (S)-cis-verbenol. To investigate the optimal field dosage of isoamyl alcohol, anhydrous ethanol was used as a solvent to combine 3 mg of (S)-cis-verbenol with different amounts of isoamyl alcohol, and the bioactivity of the rubber tree bark beetle was evaluated by varying the dosage of isoamyl alcohol in the attractant.

[0073] The rubber tree bark beetle attractant prepared in this embodiment uses commercially available (S)-cis-verbenol, isoamyl alcohol, and anhydrous ethanol. The chemical purity of (S)-cis-verbenol is greater than 95%; the chemical purity of isoamyl alcohol is greater than 99%; and the chemical purity of anhydrous ethanol is greater than 99.79%.

[0074] The following four treatments are prepared according to the testing requirements.

[0075] Treatment 1: 19.5g anhydrous ethanol, 3mg (S)-cis-verbenol, 0.3mg isoamyl alcohol;

[0076] Treatment 2: 19.5g anhydrous ethanol, 3mg (S)-cis-verbenol, 3mg isoamyl alcohol;

[0077] Treatment 3: 19.5g anhydrous ethanol, 3mg (S)-cis-verbenol, 30mg isoamyl alcohol;

[0078] Treatment 4: 19.5g anhydrous ethanol, 3mg (S)-cis-verbenol, 300g isoamyl alcohol.

[0079] The lure cores for the rubber tree bark beetle prepared in this application example are prepared using the same method as in Example 1, including the same preparation method, preservation method, and implementation steps.

[0080] Following the specific implementation steps in Example 1, a field trapping experiment was conducted in May 2024. Specific results can be found in [link to example]. Figure 3 In this experiment, each treatment was replicated three times. The number of rubber tree bark beetles captured in each treatment was the average of the three traps. Depending on the weather conditions, the number of rubber tree bark beetles in the collection bottle was observed every 5 to 7 days.

[0081] from Figure 3 It can be seen that treatments 1 through 6 all attracted rubber tree bark beetles. When different amounts of isoamyl alcohol were used in combination with (S)-cis-verbenol and anhydrous ethanol, it was found that the number of rubber tree bark beetles attracted first increased and then decreased with the increase of isoamyl alcohol. Treatments 2 and 3 significantly increased the number of rubber tree bark beetles attracted, but the difference between the two was not statistically significant. From the perspective of trapping effect and economic benefits, the combination of 3 mg of isoamyl alcohol with (S)-cis-verbenol and anhydrous ethanol has a better attraction effect on rubber tree bark beetles.

[0082] Example 4

[0083] Evaluation results of the bioactivity of different proportions of isoamyl alcohol and longifole in attractants on bark beetles of rubber trees.

[0084] In Example 2, it was found that longifolene had a significant synergistic effect on the attraction of (S)-cis-verbenol. To investigate the optimal field dosage of longifolene, anhydrous ethanol was used as a solvent to combine 3 mg of (S)-cis-verbenol with different dosages of longifolene, and the bioactivity of longifolene dosage in the attractant on the rubber tree bark beetle was evaluated.

[0085] The rubber tree bark beetle attractant prepared in this embodiment uses commercially available (S)-cis-verbenol, longifolene, and anhydrous ethanol. The chemical purity of (S)-cis-verbenol is greater than 95%; the chemical purity of longifolene is greater than 95%; and the chemical purity of anhydrous ethanol is greater than 99.79%.

[0086] The following four treatments are prepared according to the testing requirements.

[0087] Treatment 1: 19.5 g anhydrous ethanol, 3 mg (S)-cis-verbenol, 0.3 mg longleaf ethanoylene;

[0088] Treatment 2: 19.5 g anhydrous ethanol, 3 mg (S)-cis-verbenol, 3 mg longleaf ethanoylene;

[0089] Treatment 3: 19.5 g anhydrous ethanol, 3 mg (S)-cis-verbenol, 30 mg longleaf ethanoylene;

[0090] Treatment 4: 19.5 g anhydrous ethanol, 3 mg (S)-cis-verbenol, 300 mg longleaf ethoxylate;

[0091] The lure cores for the rubber tree bark beetle prepared in this application example are prepared using the same method as in Example 1, including the same preparation method, preservation method, and implementation steps.

[0092] Following the specific implementation steps in Example 1, a field trapping experiment was conducted in November 2024. Specific results can be found in [link to example]. Figure 4 In this experiment, each treatment was replicated three times. The number of rubber tree bark beetles captured in each treatment was the average of the three traps. Depending on the weather conditions, the number of rubber tree bark beetles in the collection bottle was observed every 5 to 7 days.

[0093] from Figure 4 It can be seen that treatments 1 through 6 all attracted rubber tree bark beetles. When different amounts of longifolene were used in combination with (S)-cis-verbenol and anhydrous ethanol, it was found that the number of rubber tree bark beetles attracted first increased and then decreased with the increase of longifolene dosage. Treatment 2 significantly increased the number of rubber tree bark beetles attracted, while the other three treatments were significantly less effective than treatment 2. This indicates that the combined use of 3 mg longifolene with (S)-cis-verbenol and anhydrous ethanol has a good attraction effect on rubber tree bark beetles.

[0094] Example 5

[0095] Evaluation results of the bioactivity of the attractant isoamyl alcohol in combination with longleaf olein and (S)-cis-verbenol on bark beetles of rubber trees.

[0096] Based on the results of Examples 2 and 3, the trapping effect of isoamyl alcohol combined with longifolene and (S)-cis-verbenol on rubber tree bark beetles was further investigated. Using anhydrous ethanol as a solvent, 3 mg of (S)-cis-verbenol, 30 mg of isoamyl alcohol (which has a good synergistic effect on attraction), and 3 mg of longifolene (which also has a good synergistic effect on attraction) were used in combination to evaluate the bioactivity of the combined use of isoamyl alcohol, longifolene, and (S)-cis-verbenol as attractants on rubber tree bark beetles.

[0097] The rubber tree bark beetle attractant prepared in this embodiment uses commercially available (S)-cis-verbenol, isoamyl alcohol, longifolene, and anhydrous ethanol. The chemical purity of (S)-cis-verbenol is greater than 95%; the chemical purity of isoamyl alcohol is greater than 99%; the chemical purity of longifolene is greater than 95%; and the chemical purity of anhydrous ethanol is greater than 99.79%.

[0098] The following five treatments are prepared according to the testing requirements.

[0099] Treatment 1: 19.5g anhydrous ethanol, 3mg (S)-cis-verbenol, 3mg isoamyl alcohol, 3mg longleaf ethoxylate;

[0100] Treatment 2: 19.5g anhydrous ethanol, 3mg (S)-cis-verbenol, 30mg isoamyl alcohol, and 3mg longleaf ethanoylene;

[0101] Treatment 3: 19.5g anhydrous ethanol, 3mg (S)-cis-verbenol, 3mg isoamyl alcohol;

[0102] Treatment 4: 19.5g anhydrous ethanol, 3mg (S)-cis-verbenol, 30mg isoamyl alcohol;

[0103] Treatment 5: 19.5g anhydrous ethanol, 3mg (S)-cis-verbenol, 3mg longleaf ethanoylene.

[0104] The lure cores for the rubber tree bark beetle prepared in this application example are prepared using the same method as in Example 1, including the same preparation method, preservation method, and implementation steps.

[0105] Following the specific implementation steps in Example 1, a field trapping experiment was conducted in December 2024. Specific results can be found in [link to example]. Figure 5 In this experiment, each treatment was replicated three times. The number of rubber tree bark beetles captured in each treatment was the average of the three traps. Depending on the weather conditions, the number of rubber tree bark beetles in the collection bottle was observed every 5 to 7 days.

[0106] from Figure 5 It can be seen that treatments 1 through 6 all attracted rubber tree bark beetles. When isoamyl alcohol and longifolene were used in combination with (S)-cis-verbenol and anhydrous ethanol, the number of rubber tree bark beetles captured was significantly higher than when isoamyl alcohol or longifolene alone was used in combination with (S)-cis-verbenol and anhydrous ethanol, with treatment 1 capturing the highest number of bark beetles. These results indicate that the combined use of 3 mg isoamyl alcohol and 3 mg longifolene with (S)-cis-verbenol and anhydrous ethanol has a good attractant effect on rubber tree bark beetles.

[0107] Example 6

[0108] Evaluation results of the bioactivity duration of the attractant on the rubber tree bark beetle.

[0109] The rubber tree bark beetle attractant prepared in this embodiment uses commercially available (S)-cis-verbenol, isoamyl alcohol, longifolene, and anhydrous ethanol. The chemical purity of (S)-cis-verbenol is greater than 95%; the chemical purity of isoamyl alcohol is greater than 99%; the chemical purity of longifolene is greater than 95%; and the chemical purity of anhydrous ethanol is greater than 99.79%.

[0110] Prepare one treatment as required for testing.

[0111] Treatment 1: 19.5g anhydrous ethanol, 3mg (S)-cis-verbenol, 3mg isoamyl alcohol, 3mg longleaf ethoxylate;

[0112] The lure cores for the rubber tree bark beetle prepared in this application example are prepared using the same method as in Example 1, including the same preparation method, preservation method, and implementation steps.

[0113] Following the specific implementation steps in Example 1, a field trapping experiment was conducted from January to February 2025. Specific results can be found in [link to example]. Figure 6 In this experiment, each treatment was replicated three times. The number of rubber tree bark beetles captured in each treatment was the average of the three traps. Depending on the weather conditions, the number of rubber tree bark beetles in the collection bottle was observed every three days.

[0114] from Figure 6 It can be seen that the bark beetle lure prepared by this invention can attract bark beetles for 30 days, and their occurrence dynamics can be accurately monitored. The combined use of (S)-cis-verbenol 3mg, isoamyl alcohol 3mg, longleafene 3mg, and anhydrous ethanol 19.5g in the field can have a good attraction effect on bark beetles, and the duration of attraction in the field can meet the requirements for monitoring the occurrence dynamics of bark beetles.

[0115] Although the above embodiments have provided a detailed description of the present invention, they are only some embodiments of the present invention, and not all embodiments. People can obtain other embodiments based on these embodiments without creative effort, and these embodiments all fall within the protection scope of the present invention.

Claims

1. An attractant for rubber tree bark beetles, characterized in that, The components include the following parts by weight: (S)-cis-verbenol 1 part, isoamyl alcohol 1 part, longifolene 1 part, and anhydrous ethanol 6500 parts.

2. Use of the attractant of claim 1 in attracting rubberwood ambrosia beetles.

3. An attractant core for attracting a rubberwood ambrosia beetle, characterized in that, The slow-release carrier, the absorbent cotton placed in the slow-release carrier, and the attractant of claim 1 infiltrated on the absorbent cotton.

4. The lure of claim 3, wherein, In a single lure core, the content of (S)-cis-verbenol is 3 mg; the content of isoamyl alcohol is 3 mg; the content of longifolene is 3 mg; and the content of anhydrous ethanol is 19.5 g.

5. The lure of claim 3, wherein, The slow-release carrier includes a polyethylene plastic bag.

6. The lure of claim 5, wherein, The polyethylene plastic bag has a specification of 10 silk, black, 120 mm in length, and 80 mm in width.

7. The lure of claim 3 wherein, The content of absorbent cotton in a single lure core is 5 g.

8. A method of producing the lure according to any one of claims 3 to 7, characterized in that, The method includes the following steps: The absorbent cotton is placed in the slow-release carrier, and the attractant is added dropwise on the absorbent cotton to obtain the lure core after sealing.

9. Use of the lure core of any one of claims 3-6 in attracting rubberwood ambrosia beetles.

10. Use according to claim 9, characterized in that, The use includes the following steps: the lure core is used in combination with a cross-intersection plate type ambrosia beetle trap, and is arranged at the edge of a rubber forest or in an open area in the forest with a spacing of 50 m and a height of 1.5-2 m from the ground.

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