Dietary nutrition supplement capable of relieving radon internal irradiation damage and having protection effect and preparation method of dietary nutrition supplement

Abstract

The invention relates to the technical field of radiation protection, in particular to a dietary nutrition supplement capable of relieving radon internal irradiation damage and having a protective effect and a preparation method thereof, and the dietary nutrition supplement comprises the following raw materials: compound vitamins, nicotinamide, selenium-enriched yeast and propolis. Animal experiments prove that after the dietary nutrition supplement is taken, the dietary nutrition supplement not only is beneficial to relieving oxidative damage and apoptosis resistance caused by radon irradiation, inhibiting autophagy and relieving damage of radon daughters to lung cells, but also does not generate toxic and side effects, and achieves the purposes of safety and effectiveness.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of radiation protection, in particular to a dietary nutrition supplement with protective effect for reducing radon internal radiation damage and a preparation method thereof. BACKGROUND

[0002] Radon is a natural radioactive gas in the environment, which is colorless and odorless. Radon daughter in air can form radioactive aerosols and contaminate the air, which is easily trapped by the respiratory system and forms long-term internal radiation in the body. With the continuous accumulation of inhaled dose, long-term inhalation of high concentration radon can eventually induce lung cancer. The International Agency for Research on Cancer (IARC) classifies radon and its daughter as a class I human carcinogen. WHO lists radon as one of the 19 known important human carcinogens. Radon internal radiation has long-term, complex and uncertain characteristics. On the one hand, radon is colorless and odorless, and radon daughter in air can form radioactive aerosols, which are not easily detected by detection instruments and are easily ignored. On the other hand, survey data shows that the risk of lung cancer in retired miners is high. Epidemiological surveys have confirmed that radon exposure in the living environment can also increase the risk of lung cancer, and there are many cases of lung cancer caused by radon every year.

[0003] The current control measures for radon concentration are to wear masks and ventilate the environment. However, these measures are not completely effective for workers working in high-concentration radon environments, especially in underground mining sites. In daily life, colorless and odorless radon in aerosol state is not easy to attract attention due to its long accumulation time, such as long-term exposure to radon environments such as subways, caves or nuclear submarine operating rooms. At present, the medical protection and management of radon internal radiation mainly follows the principles of external absorption and accelerated excretion. For example, after suspected or confirmed radon pollution, the most direct method in clinical practice is to encourage a large amount of water intake to accelerate its excretion by using the biological half-life of water metabolism. In some cases, diuretics may be used to further improve the excretion efficiency. However, these methods have obvious limitations: first, they belong to post-intervention and cannot provide protection before or during exposure; second, a large amount of water intake or the use of diuretics may cause electrolyte imbalance, which is contraindicated for those with heart and kidney dysfunction, and the overall effect of accelerated excretion has a ceiling effect, and has little effect on organic combined radon integrated into organic molecules.

[0004] In recent years, some natural products with strong antioxidant activity have attracted attention, such as tea polyphenols, grape seed proanthocyanidins, Ganoderma lucidum polysaccharides, and spirulina. These extracts usually contain polyphenols and polysaccharides, and their antioxidant capacity is better than that of single vitamins. Some patents or literature have reported their use in the preparation of anti-radiation foods or drugs. However, these technical solutions also face challenges: (1) Complex and unclear composition: Natural extracts are mixtures of various compounds, and their key components and mechanisms of action are often not clear enough, resulting in poor product quality control and effect stability. (2) Low bioavailability: Many polyphenols have low bioavailability after oral administration and are metabolized quickly in the body, making it difficult to reach and maintain effective concentrations in target tissues and cells. (3) Lack of specific research on radon internal irradiation: Most studies focus on acute, high-dose external irradiation, whose damage patterns are significantly different from those caused by chronic, low-dose-rate internal irradiation caused by radon. Directly applying external irradiation protection strategies to internal irradiation lacks direct evidence of their effectiveness.

[0005] While existing anti-radiation drugs are effective, their effective dosage range is small, they have serious toxic side effects, and they must be used under medical supervision. Therefore, there is an urgent need for a safe and effective dietary supplement that can protect against radon internal radiation damage. Summary of the Invention

[0006] This invention is intended for the health protection of individuals exposed to radon internal radiation, such as nuclear industry workers, medical professionals, and residents of buildings with high radon concentrations. By taking the dietary supplements of this invention, these individuals can reduce oxidative damage, mitigate radon radiation damage, and avoid the serious consequences of cumulative radon exposure.

[0007] The present invention adopts the following technical solution: The first aspect of this invention provides a dietary supplement that has a protective effect against radon internal irradiation damage, the raw materials of which include: multivitamins, nicotinamide, selenium and propolis.

[0008] As a preferred embodiment, the daily dosage is calculated based on the user's body weight in kg, and the ingredients include: 90-120 mg / kg of compound vitamins, 10-20 mg / kg of nicotinamide, 90-110 μg / kg of selenium, and 0.5-3 g / kg of propolis.

[0009] In a preferred embodiment, the multivitamin includes B vitamins and vitamin C.

[0010] In a preferred embodiment, the mass ratio of the B vitamins to vitamin C is (5-15):100.

[0011] In a preferred embodiment, the B vitamins include vitamin B1, vitamin B2, vitamin B6, and vitamin B12.

[0012] In a preferred embodiment, the mass ratio of vitamin B1, vitamin B2, vitamin B6, and vitamin B12 is (1-3):(1-3):(1-5):(1-5).

[0013] In a preferred embodiment, the propolis is black propolis.

[0014] In a preferred embodiment, the selenium is selenium-enriched yeast.

[0015] A second aspect of the present invention provides a method for preparing a dietary supplement that has a protective effect against radon internal radiation damage, comprising the following steps: S1. After grinding propolis into powder, add sodium carboxymethyl cellulose solution to obtain a suspension; S2. Add the remaining ingredients to the suspension, stir well, and then take it.

[0016] In a preferred embodiment, the sodium carboxymethyl cellulose solution has a mass fraction of 5-20%.

[0017] Propolis is a treasure trove of natural antioxidants, rich in flavonoids (such as quercetin and galangin), phenolic acids, and more. These substances possess antioxidant capabilities far exceeding those of vitamins C and E, providing a powerful and broad-spectrum free radical scavenging ability and reducing oxidative stress.

[0018] The core function of selenium in selenium-enriched yeast is not direct antioxidation, but rather as an essential trace element, constituting a key component of glutathione peroxidase. This enzyme can specifically catalyze the decomposition of hydrogen peroxide and lipid peroxides, which are among the most harmful to the human body, converting them into harmless water and alcohol.

[0019] Multivitamins, in conjunction with propolis and selenium enzymes, reduce damage. Nicotinamide, a precursor to NAD+, supports the efficient functioning of PARP enzymes, thereby promoting timely repair of DNA damage and preventing the accumulation of gene mutations. The combined action of propolis, vitamins, and selenium effectively mitigates the cumulative damage caused by radon irradiation at the cellular and molecular levels, providing protection.

[0020] The technical solution adopted in this invention can achieve the following beneficial effects: (1) This invention uses food-grade formulations and combines clinical experience to screen vitamins, minerals, macro / micro elements and food-medicine homologous substances with radiation protection effects. It also combines the "Chinese Dietary Reference Intakes" to determine each component and its concentration. The compound components are mixed according to the set ratio. The prepared dietary supplement has a safe and effective effect on patients who have been damaged by long-term low-dose radon irradiation. It can be consumed for a long time and has no toxic side effects.

[0021] (2) Animal experiments have verified that the dietary supplement of the present invention not only helps to reduce the antioxidant damage caused by radon irradiation, resist apoptosis, inhibit the occurrence of autophagy, and reduce the damage of radon progeny to lung cells, but also does not produce toxic side effects, thus achieving the purpose of safety and effectiveness. Attached Figure Description

[0022] To more clearly illustrate the technical solutions of the embodiments of the present invention, the accompanying drawings used in the description of the embodiments will be briefly introduced below, forming part of the present invention. The illustrative embodiments of the present invention and their descriptions explain the present invention and do not constitute an improper limitation of the present invention. In the accompanying drawings: Figure 1 The figure shows the effect of dietary supplements on superoxide dismutase in the lungs of mice irradiated with radon.

[0023] Figures 2-4 The figures show the effects of dietary supplements on the expression of Bax, Bcl-2, and LC3 proteins in the lungs of radon-irradiated mice.

[0024] Figure 5A -D is a diagram showing the pathological examination results of mice exposed to radon using dietary supplements. Detailed Implementation

[0025] To make the objectives, technical solutions, and advantages of this invention clearer, the technical solutions of this invention will be clearly and completely described below in conjunction with specific embodiments and corresponding drawings. In the description of this invention, it should be noted that the term "or" is generally used to include the meaning of "and / or," unless otherwise expressly indicated.

[0026] Obviously, the described embodiments are only some, not all, of the embodiments of the present invention. All other embodiments obtained by those skilled in the art based on the embodiments of the present invention without inventive effort are within the scope of protection of the present invention.

[0027] Example 1 Example 1 of this application provides a dietary supplement with a protective effect against radon internal radiation damage. The daily dosage is calculated based on the user's body weight in kg, and includes: Vitamin B1 2mg / kg, Vitamin B2 2mg / kg, Vitamin B6 2.5mg / kg, Vitamin B12 3mg / kg, Vitamin C 100mg / kg, Nicotinamide 15mg / kg, Selenium-enriched yeast 100μg / kg, Propolis 1.5g / kg.

[0028] The selenium is selected from selenium-enriched yeast produced by Angel Yeast Co., Ltd., and the propolis is selected from Northeast black propolis with a purity of 98%. Vitamins B1, B2, B6, B12, nicotinamide, and vitamin C are selected from national drug approval vitamin tablets purchased from pharmacies.

[0029] The human-to-mouse conversion factor is 12.3, and the human is calculated as 50 kg. Taking the preparation of a 20 mL dietary supplement for oral gavage in mice as an example, the preparation method is as follows: (1) Grind the propolis into powder in a mortar and pestle, and weigh out 0.16g. Add small amounts of 10% sodium carboxymethyl cellulose solution (20ml) in several batches, stirring constantly to form a suspension.

[0030] (2) Weigh out 0.026g, 0.060g, 0.033g, 0.24g, 0.045g, 0.031g, and 0.005g of vitamin B1, vitamin B2, vitamin B6, vitamin B12, nicotinamide, vitamin C, and selenium-enriched yeast, respectively.

[0031] (3) Pour the mixture from (2) into the suspension from (1), stir thoroughly, and then administer orally or by gavage.

[0032] Comparative Example 1 The specific implementation method of this comparative example is the same as that of Example 1, except that vitamin B1 is 3 mg / kg, vitamin B2 is 2.5 mg / kg, vitamin B6 is 1.5 mg / kg, and vitamin B12 is 1 mg / kg.

[0033] Comparative Example 2 The specific implementation method of this comparative example is the same as that of Example 1, except that the selenium-enriched yeast is 80 μg / kg.

[0034] Verification experiment: 1. Animal experiments 1.1 Establishing an animal model of radon irradiation: Seventy male BALB / c mice, SPF grade, aged 4-6 weeks, were divided into seven groups: a normal control group, an irradiation control group (3 groups: 120 WLM, 60 WLM, and 30 WLM), and an irradiation-administered drug group (3 groups: 120 WLM, 60 WLM, and 30 WLM), with ten mice in each group. See Table 1 for details. All animals in each dosage group underwent consistent pre-irradiation treatment to ensure uniformity in age, weight, etc.

[0035] Table 1. Animal Grouping, Number of Irradiation Days for Each Group, and Irradiation Start Schedule

[0036] Both Comparative Examples 1 and 2 were treated in the same way as the 60WLM irradiation drug group.

[0037] 1.2. Irradiation method: Radon dosing device, 1.5 × 10 6 Bq / m 3 Radon concentration, 10 hours / day, for 51, 26, and 13 consecutive days (equivalent to 120 WLM, 60 WLM, and 30 WLM doses, respectively).

[0038] 1.3. Dosage volume: 1 ml / 20 g body weight for mice.

[0039] 1.4. Daily irradiation duration: 10 hours.

[0040] 1.5. Dosage frequency: once a day.

[0041] 1.6. Dissection and tissue collection: After irradiation, the patient was euthanized by dislocation of the neck, the lungs were removed, weighed, and a portion was preserved in liquid nitrogen and another portion in formalin.

[0042] 1.7. Detection: Apoptosis-related proteins Bcl-2 and Bax, autophagy-related protein LC3, antioxidant marker superoxide dismutase (SOD), and pathology.

[0043] 1.8. Test Results: ① Effects of dietary supplements on lung weight and organ coefficients in radon-irradiated mice The method for calculating the organ coefficient is as follows: Table 2. Effects of dietary supplements on lung weight and organ coefficients in radon-irradiated mice.

[0044] As shown in Table 1, the lung organ coefficients of the 30WLM, 60WLM, and 120WLM irradiation groups were all higher than those of the corresponding irradiation control groups. This experiment demonstrates that dietary supplements have a protective effect on the lungs of animals damaged by radon irradiation.

[0045] ② Effects of dietary supplements on superoxide dismutase (SOD) levels in the lungs of radon-irradiated mice Following radon irradiation, the SOD values ​​in the 30WLM, 60WLM, and 120WLM irradiation groups all decreased, exhibiting a dose-response relationship. (See details...) Figure 1This indicates that the balance between the oxidation and antioxidant systems was disrupted, leading to oxidative stress. The SOD values ​​of the 30WLM, 60WLM, and 120WLM dietary supplement groups were all higher than those of the corresponding irradiated control groups, and there was a significant dose-response relationship. This suggests that the dietary supplement can scavenge free radicals, reduce oxidative damage to lung tissue, and play a protective role against radon-induced lung tissue damage.

[0046] Table 3. Effects of dietary supplements on SOD in radon-irradiated mice.

[0047] ③ Results of experiments on apoptosis in radon-irradiated mice using dietary supplements Bax is a pro-apoptotic protein that promotes mitochondrial outer membrane permeability, releases cytochrome C, and activates the caspase cascade. Bcl-2 is an anti-apoptotic protein that inhibits Bax activity and maintains mitochondrial membrane stability. (See Table 4 and...) Figures 2-4 As can be seen, compared with the corresponding drug-treated group, the irradiated control group showed decreased Bax expression and increased Bcl-2 expression, which may have a dose-response relationship. This indicates that dietary supplements have an anti-apoptotic effect on radon-injured mice.

[0048] Table 4. Effects of dietary supplements on the expression of Bax, Bcl-2, and LC3 proteins in the lungs of radon-irradiated mice.

[0049] ④ Results of autophagy experiment on radon-irradiated mice using dietary supplements Radiation-induced oxidative stress activates autophagy, through which cells clear damaged organelles or protein aggregates; or, persistent autophagy may lead to cell death. LC3, a key protein in autophagy, may reflect changes in autophagic activity under radiation stress. After radon irradiation, LC3 protein expression was upregulated in both the 120 WLM and 60 WLM irradiation control groups, and was higher than in the normal control group. Conversely, LC3 protein expression was downregulated in the 120 WLM and 60 WLM irradiation groups, and was lower than in the corresponding irradiation control groups. See [link to radion irradiation report]. Figure 4 .

[0050] Radon induces autophagy activation by upregulating the expression levels of autophagy-related factors such as LC3 protein. After intervention with dietary supplements to reduce radon levels, it was found that the supplements inhibited the radon-induced decrease in the expression level of the autophagy-related factor LC3 protein, with a significant trend, suggesting that radon-induced autophagy activation may be alleviated by inhibiting oxidative stress, thus reducing damage in animals.

[0051] ⑤ Pathological examination results of radon-irradiated mice after dietary supplementation Effects of drug administration on the lungs of radon-exposed mice: Compared with the blank control group, the lungs of mice irradiated with 30WLM, 60WLM, and 120WLM, as well as the drug administration groups, showed pathological changes such as thinning of alveolar septa, reduction of alveolar epithelial cells, alveolar septal rupture, and bullous formation. In the 30WLM drug administration group, there were no differences in these pathological changes compared with the 30WLM irradiated control group. In the 60WLM and 120WLM drug administration groups, these pathological changes were alleviated compared with the corresponding irradiated control groups. See details... Figures 5A-5D .

[0052] The embodiments of the present invention have been described above with reference to the accompanying drawings. However, the present invention is not limited to the specific embodiments described above. The specific embodiments described above are merely illustrative and not restrictive. Those skilled in the art can make many other forms under the guidance of the present invention without departing from the spirit and scope of the claims, and all of these forms are within the protection scope of the present invention.

Claims

1. A dietary supplement with protective effects against radon internal radiation damage, characterized in that, Its ingredients include: multivitamins, niacinamide, selenium, and propolis.

2. The dietary supplement with protective effects against radon internal irradiation damage according to claim 1, characterized in that, The daily dosage is calculated based on the user's body weight in kg. Its ingredients include: 90-120 mg / kg of multivitamins, 10-20 mg / kg of nicotinamide, 90-110 μg / kg of selenium, and 0.5-3 g / kg of propolis.

3. A dietary supplement with protective effects against radon internal radiation damage according to claim 2, characterized in that, The multivitamin includes B vitamins and vitamin C.

4. A dietary supplement with protective effects against radon internal radiation damage according to claim 3, characterized in that, The mass ratio of the B vitamins to vitamin C is (5-15):

100.

5. A dietary supplement with protective effects against radon internal radiation damage according to claim 4, characterized in that, The B vitamins include vitamin B1, vitamin B2, vitamin B6, and vitamin B12.

6. A dietary supplement with protective effects against radon internal radiation damage according to claim 5, characterized in that, The mass ratio of vitamin B1, vitamin B2, vitamin B6, and vitamin B12 is (1-3):(1-3):(1-5):(1-5).

7. A dietary supplement with protective effects against radon internal radiation damage according to claim 6, characterized in that, The propolis mentioned is black propolis.

8. A dietary supplement with protective effects against radon internal radiation damage according to claim 7, characterized in that, The selenium mentioned is selenium-enriched yeast.

9. A method for preparing a dietary supplement with protective effects against radon internal radiation damage as described in any one of claims 1-8, characterized in that, Includes the following steps: S1. After grinding propolis into powder, add sodium carboxymethyl cellulose solution to obtain a suspension; S2. Add the remaining raw materials to the suspension and stir until well mixed.

10. The preparation method according to claim 9, characterized in that, The sodium carboxymethyl cellulose solution has a mass fraction of 5-20%.

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