Application of ginseng oil body in preparation of medicine for preventing and / or treating colitis

Abstract

The invention provides an application of ginseng oil body in preparation of a medicine for preventing and / or treating colitis, and belongs to the technical field of ginseng seed oil. The detection shows that the oil core structure of the ginseng oil body mainly consists of oleic acid and linoleic acid, and is different from the triglyceride core of the oil body obtained from the existing oil crops. Through lipidomics detection and analysis, the phospholipid monomolecular layer has 335 phospholipids (positive ion mode) and 269 phospholipids (negative ion mode). Through proteomics detection and analysis, 24 kinds of Panax proteins are identified. The condition that the oil core of the ginseng oil body contains protopanaxatriol (PPT) is identified through high performance liquid chromatography-high resolution mass spectrometry. In-vitro detection shows that the ginseng oil body has anti-inflammatory and anti-oxidation effects.

Description

Technical Field

[0001] This invention relates to the field of ginseng seed oil technology, and more particularly to the application of a ginseng oil body in the preparation of a medicine for the prevention and / or treatment of colitis. Background Technology

[0002] Oil bodies (OBs) are naturally occurring oil droplet structures in plant seed cells, commonly found in oilseed crops such as soybeans, peanuts, and rapeseed. Plant oil bodies have a diameter of 0.5-2.5 μm. When subjected to external pressure, they exhibit an irregular disk shape; however, within a given space, they are typically spherical. The core of plant oil bodies (OBs) is primarily composed of triglycerides (TAG, accounting for 94.0-98.0% of the core lipids), with small amounts of diglycerides, free fatty acids, and vitamin E. In addition, OBs contain various bioactive components, such as phytosterols, tocopherols, essential amino acids, isoflavones, and minor proteins including dioxygenases and lipoxygenases. The presence of these substances can lower cholesterol levels, neutralize free radicals, regulate hormone levels, and help reduce the risk of cardiovascular disease, aging, and certain chronic diseases.

[0003] Plant oils, with their natural phospholipid-oil protein complex membrane structure, are widely used in cosmetics and emulsification. In the food industry, they are natural complex emulsions with excellent emulsifying properties, serving as a natural alternative to artificial oil-in-water emulsions. They are widely used in emulsified products such as beverages, dairy products, chocolate, and ice cream. Therefore, plant oils have broad application prospects because they can meet consumers' demand for purely natural foods.

[0004] Currently, ginseng seed oil is known to be rich in ginsenosides, fatty acids, and antioxidants, possessing properties such as nourishing the skin, regulating metabolism, and anti-inflammatory and antioxidant effects. It is generally used for skincare, relieving fatigue, and promoting bodily recovery. Therefore, the development of applications for ginseng seed oil is still quite limited.

[0005] Based on this, the present invention is proposed. Summary of the Invention

[0006] To develop new applications for ginseng oil, the present invention provides the following technical solution: This invention provides the use of ginseng oil in the preparation of medicaments for the prevention and / or treatment of colitis.

[0007] Preferably, the ginseng oil is extracted from ginseng seeds, and its main components include 9 kinds of unsaturated fatty acids, phospholipid monolayer, protopanaxadiol, and 24 kinds of ginseng proteins.

[0008] Preferably, the ginseng seeds are fresh or dried seeds.

[0009] Preferably, the ginseng oil is prepared by the following method: ginseng seeds are soaked in PBS solution for 7-10 days, then ground and extracted using PBS solution as the extractant, and the upper semi-solid layer is taken after centrifugation to obtain the ginseng oil.

[0010] Preferably, the PBS solution needs to be pre-cooled at a temperature of 4-10°C; the amount of PBS solution added is 8-12 times the volume of the ginseng seeds.

[0011] Preferably, the grinding method is grinding with a grinding machine for 1 to 3 minutes.

[0012] Preferably, the centrifugation speed is 400-1500 rpm, the temperature is 4-10℃, and the time is 15-30 min.

[0013] This invention also provides the application of ginseng oil in the preparation of moisturizing, repairing, and anti-aging cosmetic products.

[0014] This invention extracts ginseng oil from ginseng seeds and identifies its components using various methods. The core structure of the ginseng oil is found to be mainly composed of oleic acid and linoleic acid, unlike the triglyceride core of oil obtained from oilseed crops. Lipidomics analysis revealed 335 phospholipids (positive ion mode) and 269 phospholipids (negative ion mode) in the phospholipid monolayer. Proteomics analysis identified 24 ginseng proteins. High-performance liquid chromatography-high-resolution mass spectrometry (HPLC-MS / MS) confirmed the presence of protopanaxadiol (PPT) within the ginseng oil core.

[0015] In vitro testing revealed that ginseng oil possesses strong antioxidant capabilities, with its antioxidant effect directly proportional to dosage. Further testing showed that ginseng oil can scavenge hydroxyl and DPPH free radicals. Oral treatment and preventative treatment of mice with colitis demonstrated that ginseng oil can prevent and treat colitis, proving its effectiveness in preventing and treating colitis. Skin application to healthy volunteers revealed that ginseng oil also has moisturizing, repairing, and anti-aging effects. Therefore, the ginseng oil provided by this invention can be used in oral formulations for the prevention and treatment of colitis, and in topical formulations for use in cosmetic products with moisturizing, repairing, and anti-aging effects. Attached Figure Description

[0016] Figure 1 Transmission electron microscopy imaging results of ginseng seed slices; Figure 2 A flowchart illustrating the preparation process of ginseng oil. Figure 3 The results of fatty acid content analysis in ginseng oil body; Figure 4 The results of lipidomics analysis of ginseng oil bodies (positive ion model); Figure 5 The results of lipidomics analysis of ginseng oil bodies (negative ion model); Figure 6 For the detection of ginsenosides in ginseng oil using liquid chromatography-mass spectrometry (LC-MS); Figure 7 The electrophoresis results for ginseng oil bodies are shown in the image. Figures 1 and 2 are two replicates of ginseng oil body samples. Figure 8 The results of proteomics analysis of ginseng oil bodies; Figure 9 The results show the particle size distribution of ginseng oil particles in PBS solution. Figure 10 The results show the surface potential of ginseng oil in PBS solution. Figure 11 Results of in vitro detection of hydroxyl radical scavenging in ginseng oil; Figure 12 The results of a study on the prevention of colitis in mice by oral administration of ginseng oil bodies; Figure 13 The results of a study on the treatment of colitis in mice with oral ginseng oil bodies; Figure 14 Results of the test on the moisture content of the stratum corneum of the volunteer's skin; Figure 15 The results of transepidermal water loss detection on volunteers' skin; Figure 16 Evaluation of the effectiveness of the product for volunteers. Detailed Implementation

[0017] The technical solutions provided by the present invention will be described in detail below with reference to the embodiments, but they should not be construed as limiting the scope of protection of the present invention.

[0018] Example 1

[0019] Identification of ginseng oil in ginseng seed slices

[0020] 1.1 Experimental Methods: (1) Sample preparation: Select suitable seeds and ensure they are fresh and uncontaminated. Wash the seeds in clean water to remove surface impurities.

[0021] (2) Fixation: Place the cleaned seeds in a fixative (2.5% glutaraldehyde) and fix for 2 hours at room temperature. After fixation, rinse three times with PBS buffer for 10 minutes each time to remove excess fixative.

[0022] (3) Detection: Ginseng seed samples were sliced ​​using a slicer with a slice thickness of 10µm. Microscopic imaging was performed using a transmission electron microscope (Hitachi HT7800).

[0023] 1.2 Experimental Results: like Figure 1 As shown, after scanning the ginseng seed slices with a transmission electron microscope, it can be found that the cells in the ginseng seed structure contain many irregular round vesicle structures, which are ginseng oil bodies.

[0024] Example 2

[0025] Preparation of ginseng oil

[0026] 2.1 Experimental Methods: (1) Soak ginseng seeds in 10 times the volume of pre-cooled (4°C) PBS solution for 10 days, then use PBS solution as the extraction agent and grind the seeds for 2 minutes.

[0027] (2) After centrifuging at 1000 rpm and 4℃ for 30 minutes, the upper semi-solid layer is taken to obtain ginseng oil.

[0028] 2.2 Experimental Results: After extracting ginseng seeds according to the above method, as follows Figure 2 As shown, a white semi-solid is finally obtained, which is the ginseng oil body described in this invention, wherein the ginseng oil body extraction rate (w / 100g seed%) is 2-5%.

[0029] Example 3

[0030] Detection of unsaturated fatty acids in ginseng oil

[0031] 3.1 Experimental Methods: (1) Experimental apparatus Table 1 Experimental Apparatus

[0032] (2) Experimental reagents

[0033] Table 2 Experimental Reagents

[0034] (3) Sample pretreatment methods

[0035] Sample hydrolysis: Weigh 5 mg of the homogeneous sample (prepared in Example 2), add 100 mg of pyrogallic acid, a few boiling chips, and 2 mL of 95% ethanol, and mix well. Add 10 mL of 6% hydrochloric acid solution and mix well. Place the flask in a 75°C water bath for hydrolysis for 40 min. Shake the flask every 10 min to incorporate particles adhering to the flask wall into the solution. After hydrolysis, remove the flask and cool it to room temperature.

[0036] Extraction of fat: After hydrolysis, add 10 mL of 95% ethanol and mix well. Transfer the hydrolysate from the flask to a separatory funnel, rinse the flask and stopper with 50 mL of a 1:1 mixture of diethyl ether and petroleum ether, and add the rinse solution to the separatory funnel. Cover the funnel. Shake for 5 min and let stand for 10 min. Collect the ether extract in a 250 mL flask. Repeat the above steps to extract the hydrolysate 3 times. Finally, rinse the separatory funnel with the 1:1 mixture of diethyl ether and petroleum ether and collect the extract in a pre-weighed flask. Evaporate the flask to dryness in a water bath and dry it in an oven at 100℃±5℃ for 2 h to obtain the fat extract.

[0037] Saponification and fatty acid methyl esterification of fats: Add 2 mL of 2% sodium hydroxide methanol solution to the fat extract, and incubate at 85°C for 30 min. Then add 3 mL of 14% boron trifluoride methanol solution and incubate at 85°C for another 30 min. After the water bath, allow the temperature to cool to room temperature, add 3 mL of n-hexane to a centrifuge tube, shake to extract for 2 min, and let stand for one hour to allow for layering. Filter the supernatant through a 0.45 μm filter membrane for analysis.

[0038] (4) Instrumentation Method

[0039] Chromatographic column: HP-88 (100m × 0.25mm × 0.20μm); Temperature program: Hold at 100℃ for 15 min, increase to 190℃ at a rate of 20℃ / min, hold for 6 min, then increase to 220℃ at a rate of 1℃ / min, hold for 7 min; Inlet temperature: 260℃; Carrier gas: nitrogen, flow rate: 10mL / min; Split injection, split ratio: 20:1; Detector: FID; Detector temperature: 250℃.

[0040] (5) Data calculation and processing

[0041] The content of each fatty acid in the sample is calculated according to the formula:

[0042] In the formula:

[0043] W—The content of each fatty acid in the sample, in g / 100g; C—Concentration of fatty acid methyl esters in the sample solution, in mg / L; V – constant volume, in mL; k—conversion factor for the conversion of each fatty acid methyl ester to fatty acid, see Table 3 below; N – Dilution factor; 10 -4 —Unit conversion factor; m — the mass of the sample, in grams.

[0044] Table 3. Summary of fatty acid types, detection limits, and conversion factors for converting fatty acid methyl esters or fatty acid triglycerides to fatty acids.

[0045] 3.2 Experimental Results: The 37 fatty acids in ginseng oil were qualitatively and quantitatively detected by high performance liquid chromatography (HPLC), and the results are shown in Table 4. A total of 9 fatty acid components were detected. Among them, oleic acid accounted for 79.62%, and linoleic acid accounted for 16.99% (e.g., ...). Figure 3 This indicates that the oil core structure of ginseng oil bodies is mainly composed of oleic acid and linoleic acid, rather than triglycerides. This result was further validated by lipidomics analysis.

[0046] Table 4. Quantitative results of 37 fatty acids in ginseng oil.

[0047] Example 4

[0048] 4.1 Experimental Methods

[0049] (1) Experimental reagents and instruments

[0050] Table 5. Reagents for Lipomics Experiments

[0051] Table 6. Lipomics Experimental Instruments

[0052] (2) Experimental methods

[0053] The main sample extraction process is as follows: 1) Take 100 µL of ginseng oil sample, add 400 µL of methanol:dichloromethane = 2:1 (v / v), and vortex for 20s; 2) Add 150 µL H2O and 150 µL dichloromethane, vortex for 20 s, equilibrate at 22 ℃ for 10 min, and then centrifuge at 12000 rpm at 22 ℃ for 10 min. 3) Collect the lower dichloromethane layer, dry it with nitrogen, redissolve it with 500 µL of mobile phase (acetonitrile:isopropanol:water = 65:30:5), filter it through a 0.22 µm microporous membrane, and add it to a brown vial; 4) Sampling and on-machine testing ① Instrument analysis platform: LC-MS (Thermo, Vanquish, Q Exactive HF) ②Chromatographic column: C18 column (Zorbax Eclipse C18 (1.8μm)) 2.1mm 100mm); ③Chromatographic separation conditions: Column temperature: 35 °C; Flow rate: 0.25 mL / min; Injection volume: 2 μL; Mobile phase: (A) 60 / 40 acetonitrile / water, containing 0.1% formic acid and 10 mM ammonium formate; (B) 90 / 10 isopropanol / pure acetonitrile, containing 0.1% formic acid and 10 mM ammonium formate; Autosampler temperature: 4 °C. The gradient elution program for the mobile phase is shown in Table 7.

[0054] Table 7 Mobile phase conditions for liquid chromatography

[0055] Positive mode: heater temperature 325 °C; sheath flow rate: 35 arb; auxiliary flow rate: 12 arb; purge flow rate: 0 arb; electrospray voltage: 4.35 kV; capillary temperature: 330 °C; S-Lens RF Level: 55%.

[0056] Negative mode: heater temperature 325 °C; sheath flow rate: 35 arb; auxiliary flow rate: 12 arb; purge flow rate: 0 arb; electrospray voltage: 4.0 kV; capillary temperature: 330 °C; S-Lens RF Level: 55%.

[0057] Scanning modes: Full Scan (m / z 200~1450) and data-dependent secondary mass spectrometry (dd-MS2, TopN = 5); resolutions 240,000 (primary mass spectrometry) & 60,000 (secondary mass spectrometry).

[0058] Collision mode: High-energy collision dissociation (HCD).

[0059] 4.2 Experimental Results

[0060] 335 lipids were detected in positive ion mode and 269 lipids were detected in negative ion mode (e.g. Figure 4 , Figure 5 According to the Chinese classification of lipids, fatty acids, pregnenolone lipids, polyethylene lipids, sphingolipids, sterol lipids, glycerides, glycerophospholipids, and glycolipids were detected.

[0061] Meanwhile, liquid chromatography-mass spectrometry (LC-MS) detected that ginseng oil contains one type of ginsenoside (Ginsenoside 1). Figure 6 The molecule, with a molecular weight of 477 Da, was identified as protopanaxadiol (PPT) by mass spectrometry. Its structural formula is as follows:

[0062] Example 5

[0063] Results of ginseng oil proteomics analysis

[0064] 5.1 Experimental Methods

[0065] (1) Experimental reagents and instruments

[0066] Table 8. Proteomics Reagents

[0067] Table 9 Proteomics Instruments

[0068] (2) Experimental methods

[0069] 1) SDS-PAGE

[0070] 1. Use the Omni-Easy™ one-step PAGE gel rapid preparation kit. Take equal volumes of the lower layer gel solution and lower layer gel buffer, 2.7 mL each, and mix well; 2. Take equal volumes of the upper layer gel solution and the colored upper layer gel buffer, 0.75 mL each, and mix well; 3. Add 60 μL of the modified coagulant to the mixed solution in step 1, mix gently, and pour the mixed solution into the gel-forming glass plate, so that the distance between the liquid surface and the upper edge of the short glass plate is 0.5 cm longer than the comb teeth. 4. Add 15 μL of the modified coagulant to the mixed solution in step 2, mix gently, and without waiting for the lower layer of gel to solidify, slowly pour the mixed solution into the gel-forming glass plate and insert the comb teeth. 5. After the gel has solidified (about 15 minutes), remove the comb teeth and it is ready for electrophoresis.

[0071] 6. Ginseng oil was diluted with PBS at a volume ratio of 1:10. 40 µL of the sample was mixed with 10 µL of 5X electrophoresis loading buffer at a ratio of 4:1. The sample was boiled in a metal bath at 100 °C for 10 min. 10 µL of the prepared sample was then loaded onto the metal bath. Electrophoresis was performed at 120 V for 50 min, followed by Coomassie brilliant blue staining.

[0072] 2) Preprocessing

[0073] After rinsing the electrophoresis gel thoroughly with water, cut the target bands (ensuring each band is as uniform in size and shape as possible) into 1 mm square pieces and place them in EP tubes. Add an appropriate volume of acetonitrile dry gel, followed by approximately 50 μL of DTT solution (10 mM, dissolved in 100 mM ammonium bicarbonate) to completely cover the dry gel particles. Incubate at 56°C for 1 hour. After cooling to room temperature, add pure acetonitrile dry gel and discard all solvents. Then add approximately 50 μL of IAA solution (55 mM, dissolved in 100 mM ammonium bicarbonate) to cover the dry gel particles and incubate in the dark for 50 minutes. After cooling to room temperature, add pure acetonitrile dry gel and discard all solvents. Finally, add a sufficient volume of trypsin solution (10 ng / μL) for overnight enzymatic digestion. After enzymatic hydrolysis, a 50% acetonitrile solution containing 0.1% formic acid, an 80% acetonitrile solution containing 0.1% formic acid, and pure acetonitrile were added sequentially to extract the peptides from the colloidal particles. The extracted peptides were then combined and freeze-dried.

[0074] 2) Chromatography-mass spectrometry analysis

[0075] Solution A used in the liquid chromatography was a 0.1% formic acid aqueous solution, and solution B was a 0.1% formic acid-acetonitrile aqueous solution (acetonitrile content 80%). The liquid chromatography column was 75 μm. 2cm, Acclaim PepMap™ 100 (Thermo Scientific Technology Inc.) and liquid chromatography column (75μm) The 25cm AcclaimPepMap™ RSLC (Thermo Scientific Technology Inc.) was equilibrated with 97% solution A at a volume of 1 μL. After pre-column loading, separation was achieved by elution from the analytical column. The relevant liquid phase gradient settings were as follows: 0 min–3 min, linear gradient of solution B from 3% to 8%; 3 min–45 min, linear gradient of solution B from 8% to 28%; 45 min–54 min, linear gradient of solution B from 28% to 38%; 54 min–56 min, linear gradient of solution B from 38% to 100%; 56 min–60 min, solution B maintained at 100%.

[0076] 3) Mass spectrometry identification

[0077] The enzymatic hydrolysis products were separated by capillary high-performance liquid chromatography (HPLC) and then analyzed by mass spectrometry using a Thermo QE HF mass spectrometer (Thermo Fisher). Analysis time: 60 min. Detection mode: positive ion. The mass-charge ratio of the peptide and peptide fragments was acquired using the following method: 20 fragment spectra were acquired after each full scan (MS² scan). Scan range: 400-1800, primary resolution: 60000, secondary resolution: 15000, collision CE: 28 eV.

[0078] 4) Data Analysis

[0079] The raw mass spectrometry test file was searched in the relevant database using the software Proteome Discoverer 2.5 to obtain the protein identification results. The relevant parameters and explanations used for the database search are shown in Table 10 below.

[0080] Table 10 Parameters used for database lookup in Proteome Discoverer

[0081] 5.2 Experimental Results

[0082] The results of the analysis of ginseng oil bodies by 10% SDS-PAGE electrophoresis are as follows: Figure 7 Peptide segment comparison using a protein library search identified 24 ginseng proteins. The molecular weights of these proteins ranged from 17.8 kDa to 221.8 kDa (e.g., ...). Figure 8 ).

[0083] Example 6

[0084] Ginseng oil particle size analysis results

[0085] 6.1 Experimental Methods

[0086] (1) Sample preparation: Add the extracted ginseng oil sample to 10 times the volume of PBS solution and mix by blowing.

[0087] (2) Testing: The Marlven 3000 laser particle size analyzer was used to test the samples in HydroVL mode, and the test was repeated 3 times.

[0088] 6.2 Experimental Results

[0089] like Figure 9 , Figure 10 As shown, ginseng oil particles exhibit a uniform distribution with a particle size of 342.2 ± 15.5 nm in PBS. The surface displays a negative charge with a potential of -29.13 ± 0.21 mV.

[0090] Example 7

[0091] Detection results of antioxidant activity of ginseng oil

[0092] 7.1 Experimental Methods

[0093] 1. Experimental materials: (1) Terephthalic acid (TA): 10 mmol of stock solution, dissolved in 10 ml of water, final concentration 0.5 mmol (diluted with PBS).

[0094] (2) 30% H2O2: 100 mmol of mother liquor, diluted with Milli-Q to a final concentration of 10 ml.

[0095] (3) FeSO4: 50 mmol of mother liquor, dissolved in ddH2O to 5 ml.

[0096] (4) Ginseng oil sample: stock solution 5 mg / ml, concentration gradient: 5 × 10⁻⁶ -1 mg / ml ~5×10 -6 mg / ml; (5) Positive control: VC stock solution 1 mg / ml, concentration gradient 1×10 -1 mg / ml ~ 1×10 -6 mg / ml.

[0097] 2. Experimental Procedure: (1) Take 1 mL of TA stock solution, 200 μL of H2O2 stock solution, 400 μL of FeSO4 stock solution, and 8.4 mL of PBS to prepare a 10 mL mixture. Select wells A to F, 1 to 6, and add 100 μL of sample volume and 100 μL of mixture to each well according to the concentration gradient; select wells A to F, 7 to 12, and add 100 μL of vitamin C volume to each well according to the concentration gradient.

[0098] (2) After mixing 100 μL of the mixture, incubating at 37°C for 2 hours can capture OH-. - Fluorescence is produced by hydroxyterephthalic acid: excitation wavelength: 320nm, emission wavelength: 425nm.

[0099] 7.2 Experimental Results

[0100] like Figure 11 As shown, ginseng oil has the ability to scavenge hydroxyl free radicals, and its scavenging effect is directly proportional to the concentration. A concentration of 10 mg / ml can scavenge about 60% of strong free radicals in the reaction system.

[0101] Example 8

[0102] Results of ginseng oil in the prevention and treatment of colitis

[0103] 8.1 Experimental Methods

[0104] 1) Autoclave all drinking water bottles and bottle caps with 1.5L of ddH2O. After cooling to room temperature, prepare 3% DSS solution. Fill each bottle with approximately 300ml. Change the solution every two days.

[0105] 2) Ear tags were used to mark C57 mice, and their weight was measured and recorded.

[0106] 3) The mice were divided into cages, with 3 mice per cage.

[0107] 4) Modeling method: Mice were deprived of water for 6 hours before administration. Administration: Mice were allowed free access to water (containing 3% DSS), and the daily water intake was observed. Administration was repeated for 7 consecutive days, followed by another 5 days. DAI scores were recorded daily.

[0108] 5) Mouse grouping: PBS group: 6 animals. After 7 days of modeling, the modeling was stopped, and PBS solution was administered by gavage for another 5 days. Ginseng OB prevention group (10mg / kg): The drug was administered while the model was being established. Ginseng oil was administered by gavage for 7 days while the model was being established by drinking 3% DSS solution. Positive drug 5-AGA (100mg / kg) treatment group: The drug was administered after modeling. Seven days after modeling, modeling was stopped, and the positive drug solution was administered by gavage for another 5 days. Ginseng OB treatment group (10mg / kg): The drug was administered after modeling. After 7 days of modeling, the modeling was stopped, and ginseng oil was administered by gavage for another 5 days.

[0109] 8.2 Experimental Results

[0110] Evaluation of multiple indicators in mice, including body weight, fecal matter, and colon length, revealed that oral administration of ginseng oil can prevent the occurrence and progression of colitis. Figure 12 As shown, compared with the model group, the prevention group experienced slower weight loss but rapid weight recovery after modeling was stopped. Simultaneously, the DAI score was lower in the prevention group, and anatomical sampling revealed that the colon length was 1.5 cm longer in the prevention group.

[0111] like Figure 13 As shown, after successful establishment of the colitis model, mice were treated orally with the positive control drug 5-hydroxysalicylic acid (5-ASA) and ginseng oil. Ginseng oil showed comparable efficacy to the positive control drug in terms of weight recovery, DAI score, and colon length recovery. This further demonstrates that oral administration of ginseng oil has a therapeutic effect on colitis.

[0112] Example 9

[0113] Application of ginseng oil in topical skin treatment

[0114] 1) Detection of moisture content in the stratum corneum of volunteer skin

[0115] The water content of the stratum corneum on the inner forearm of 10 volunteers was measured using a Coroneometer (CM825) probe. The test sample was measured at (2.0±0.1) mg / cm³. 2 The dosage was applied in a single application, with each application being approximately 10 mg. The stratum corneum moisture content in the control area and test area at 0 h, 1 h, 2 h, 4 h, and 6 h was measured using a rating scale (0-100 AU).

[0116] like Figure 14 As shown, the sample group with the applied sample significantly increased the moisture content of the stratum corneum starting 1 hour later, and the effect lasted for at least 4 hours. In contrast, the blank group without the applied sample did not show a significant change in the moisture content of the stratum corneum. This indicates that the sample can significantly increase the moisture content of the stratum corneum and has a very good skin moisturizing effect.

[0117] Transepidermal water loss (TEWL) in volunteers' skin

[0118] The test area on the inner forearm of the volunteer was measured using a Tewameter (TM300) probe, with the test sample administered at a concentration of (2.0 ± 0.1) mg / cm². 2 The drug was applied in a single application, with each application being approximately 10 mg. TEWL (transient endothelial vascularization) was measured in the control and test areas at 0 h, 1 h, 2 h, and 4 h, and the average value was recorded. The probe was cleaned between measurements. The barrier protection efficacy was evaluated by comparing the changes in the average skin moisture loss in the control and test areas, using a rating scale (0 to 90 g / m²). 2 / h) represents the measured value.

[0119] like Figure 15 As shown, the skin TEWL value of the sample group was significantly reduced starting 1 hour after application, and the effect lasted for at least 2 hours. In contrast, the skin TEWL value of the blank group without sample application did not change significantly. This indicates that the sample can significantly reduce transepidermal water loss and strengthen the skin barrier function.

[0120] 3) Evaluation of the effects of using the face mask by volunteers

[0121] Thirty volunteers aged 30-50 with no obvious facial skin problems were selected. The test product was a facial mask containing 10% of the sample (formula: 90% deionized water and 10% ginseng oil soaked in the mask sheet). One mask was used every three days for two months. A questionnaire was used to assess the volunteers' evaluation of the effects after use. The results are as follows: Figure 16 As shown.

[0122] like Figure 16As shown, after volunteers used the sample-containing mask for 2 months, they found that it significantly increased skin elasticity, brightened skin color, and reduced wrinkles around the eyes and nasolabial folds, indicating that the sample has a very significant anti-skin aging effect.

[0123] As shown in the above examples, the core structure of ginseng oil is mainly composed of oleic acid and linoleic acid, which differs from the triglyceride core of oil obtained from oil crops in existing methods. Lipidomics detection and analysis revealed 335 phospholipids (positive ion mode) and 269 phospholipids (negative ion mode) in the phospholipid monolayer. Proteomics detection and analysis identified 24 ginseng proteins. High-performance liquid chromatography-high-resolution mass spectrometry identified protopanaxadiol (PPT) within the core of the ginseng oil. In vitro testing revealed that ginseng oil possesses strong antioxidant capabilities, scavenging hydroxyl and DPPH free radicals. Oral administration and preventative treatment of colitis in mice showed that ginseng oil can prevent and treat colitis. Skin application to healthy volunteers revealed that ginseng oil has moisturizing, repairing, and anti-aging effects.

[0124] The above description is only a preferred embodiment of the present invention. It should be noted that for those skilled in the art, several improvements and modifications can be made without departing from the principle of the present invention, and these improvements and modifications should also be considered within the scope of protection of the present invention.

Claims

1. The use of a ginseng oil body in the preparation of a medicament for the prevention and / or treatment of colitis.

2. The application as described in claim 1, characterized in that, The ginseng oil is extracted from ginseng seeds and its main components include 9 kinds of unsaturated fatty acids, phospholipid monolayer, protopanaxadiol and 24 kinds of ginseng proteins.

3. The application as described in claim 2, characterized in that, The ginseng seeds mentioned are either fresh or dried.

4. The application as described in any one of claims 1 to 3, characterized in that, The ginseng oil was prepared by the following method: ginseng seeds were soaked in PBS solution for 7-10 days, then ground and extracted using PBS solution as the extraction solvent. After centrifugation, the upper semi-solid layer was taken to obtain the ginseng oil.

5. The application as described in claim 4, characterized in that, The PBS solution needs to be pre-cooled at a temperature of 4-10°C; the amount of PBS solution used is 8-12 times the volume of the ginseng seeds.

6. The application as described in claim 4, characterized in that, The grinding is performed by a grinding machine at high speed, and the time is 1 to 3 minutes.

7. The application as described in claim 4, characterized in that, The centrifugation speed is 400-1500 rpm, the temperature is 4-10℃, and the time is 15-30 min.

8. The use of ginseng oil as described in any one of claims 1 to 7 in the preparation of moisturizing, repairing, and anti-aging cosmetic products.

9. The use of ginseng oil in any one of claims 1 to 7 in the preparation of antioxidant and anti-inflammatory oral products.

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