Transcription factor cuLQW1 of fargesia spiciformis and coding gene and application thereof

Abstract

The application discloses a transcription factor CuLQW1 of Chimonocalamus jinfushanensis and an encoding gene and application thereof, belongs to the technical field of plant genetic engineering, and particularly relates to an encoding gene of the transcription factor CuLQW1 of Chimonocalamus jinfushanensis, wherein the nucleotide sequence of the encoding gene is shown in SEQ ID NO. 2. The application discloses an application of the encoding gene of the transcription factor CuLQW1 of Chimonocalamus jinfushanensis in regulating synthesis of lignin and cellulose of Gramineae plants. The application discloses an application of the encoding gene of the transcription factor CuLQW1 of Chimonocalamus jinfushanensis in regulating drought stress tolerance of rice. The application successfully separates and identifies the lignin and cellulose synthesis regulation transcription factor CuLQW1 and the encoding gene thereof from Chimonocalamus jinfushanensis, finds that the lignin and cellulose synthesis regulation transcription factor CuLQW1 is involved in regulation of synthesis of lignin and cellulose, regulates drought stress tolerance of rice, and provides a new gene resource for plant material improvement genetic engineering.

Description

Technical Field

[0001] This invention belongs to the field of plant genetic engineering technology, and in particular relates to the transcription factor CuLQW1 of Phyllostachys edulis var. jinfoshanensis, its encoding gene, and its applications. Background Technology

[0002] Jinfo Mountain square bamboo ( Chimonobambusa utilis (Keng) PC Keng is a native bamboo species in China with both economic and ecological value. Its bamboo shoots are crisp, delicious, and nutritious, earning it the title of "King of Bamboo Shoots." A distinctive industry has developed around fresh bamboo shoots, dried bamboo shoots, and their processed products. Meanwhile, the Jinfo Mountain square bamboo has a unique culm shape and tough texture, making it widely used in construction, furniture, and bamboo handicrafts. In plant cell walls, cellulose, hemicellulose, and lignin constitute the cell wall skeleton. The content and spatial distribution of lignin and cellulose are key factors affecting the strength, toughness, and processing performance of bamboo. Studies have shown that regulating the synthesis and deposition of lignin and cellulose in cells can not only alter cell wall thickness and density, thereby improving the mechanical properties of bamboo such as bending and compressive strength, but also potentially enhance the plant's tolerance to abiotic stresses such as drought and high salinity. This is of great significance for the high-performance utilization and high-resistance breeding of bamboo.

[0003] Existing research on genes regulating cell wall metabolism in gramineous crops and some bamboo species has largely focused on the functional analysis of structural enzyme genes or a few transcription factors. Results indicate that transcription factors play a regulatory role in cell wall formation and wood property development by controlling the expression of key enzyme genes in downstream lignin and cellulose synthesis pathways. Overall, research on the fine-grained regulation of lignin and cellulose synthesis by bamboo transcription factors is still in its early stages, especially in *Phyllostachys japonicus*, where transcriptional regulation studies are lacking, and a systematic analysis of the cell wall regulatory network is even more scarce. The cloning, expression characteristics, and biological functions of transcription factors regulating lignin synthesis in *Phyllostachys japonicus* have not yet been publicly reported, and their mechanisms of action in cell wall component accumulation, wood property development, and stress resistance regulation remain a blank. Therefore, it is necessary to discover and identify key candidate genes for lignin and cellulose synthesis, including MYB-type transcription factors, from *Phyllostachys japonicus*. By using modern biotechnology methods such as gene overexpression, expression suppression, or gene editing, the properties of *Phyllostachys japonicus* and other bamboo species can be improved in a targeted manner. This will cultivate new germplasm with both excellent bamboo properties and strong stress resistance, providing a reliable molecular breeding foundation for the sustainable development of the *Phyllostachys japonicus* industry. Summary of the Invention

[0004] To solve the above technical problems, the application provides a bamboo transcription factor CuLQW1 in Jinfo Mountain and an encoding gene and application thereof, and overexpression of the bamboo transcription factor CuLQW1 in Jinfo Mountain or the encoding gene thereof in rice can effectively increase the lignin and cellulose content in rice, thereby providing a new genetic resource for plant material improvement genetic engineering.

[0005] To achieve the above object, the application provides an encoding gene of the bamboo transcription factor CuLQW1 in Jinfo Mountain, wherein the nucleotide sequence of the encoding gene is shown in SEQ ID NO. 2.

[0006] The application also provides the bamboo transcription factor CuLQW1 in Jinfo Mountain encoded by the encoding gene, wherein the amino acid sequence of the transcription factor CuLQW1 is shown in SEQ ID NO. 1.

[0007] The application also provides a biological material comprising the encoding gene.

[0008] Preferably, the biological material is one or more of an expression cassette, an expression vector or an engineering bacterium.

[0009] The application also provides application of the encoding gene, the bamboo transcription factor CuLQW1 in Jinfo Mountain or the biological material in regulation of lignin and cellulose synthesis of plants in Gramineae.

[0010] Preferably, the plant in Gramineae is rice.

[0011] Preferably, the expression amount of the bamboo transcription factor CuLQW1 in Jinfo Mountain is increased by overexpression of the encoding gene, so as to increase the lignin and cellulose content in the plant.

[0012] The application also provides application of the encoding gene, the bamboo transcription factor CuLQW1 in Jinfo Mountain or the biological material in regulation of drought stress tolerance of rice, and the drought stress tolerance of the rice plant is increased by increasing the expression amount of the bamboo transcription factor CuLQW1 in Jinfo Mountain through overexpression of the encoding gene.

[0013] The application also provides a method for increasing the lignin and cellulose content in rice, comprising: overexpression of the encoding gene or the bamboo transcription factor CuLQW1 in Jinfo Mountain in rice, so as to increase the lignin and cellulose content in rice.

[0014] The application also provides a method for increasing the drought stress tolerance of rice, comprising: overexpression of the encoding gene or the bamboo transcription factor CuLQW1 in Jinfo Mountain in rice, so as to increase the drought stress tolerance of the rice plant.

[0015] Compared with the prior art, the application has the following advantages and technical effects:

[0016] 1、The application successfully separates and identifies a lignin and cellulose synthesis regulatory transcription factor CuLQW1 and its coding gene from the bamboo of Jinfo Mountain, and transgenically transfers the coding gene into wild type rice to verify the function, and the lignin and cellulose content of the obtained transgenic rice is increased by about 13.0% and 14.0% respectively compared with that of the wild type rice, indicating that the transcription factor CuLQW1 is involved in the regulation of lignin and cellulose synthesis. The drought stress tolerance of the obtained transgenic rice is significantly improved compared with that of the wild type rice, indicating that the coding gene of the transcription factor CuLQW1 regulates the drought stress tolerance of rice.

[0017] 2、The application overexpresses the transcription factor CuLQW1 or its coding gene in monocotyledon model plant rice, which can effectively increase the lignin and cellulose content in rice and improve the drought stress tolerance of rice, providing a theoretical basis and technical support for subsequent improvement of the quality of Jinfo Mountain bamboo by genetic engineering technology. BRIEF DESCRIPTION OF DRAWINGS

[0018] In order to more clearly illustrate the technical solutions in the embodiments of the present application or the prior art, the drawings needed in the embodiments will be briefly introduced below. Obviously, the drawings in the following description are only some embodiments of the present application, and other drawings can also be obtained by those skilled in the art without creative labor on the basis of these drawings.

[0019] Figure 1 It is a pC1300-CuLQW1 recombinant plasmid double enzyme cutting agarose gel electrophoresis chart, in which M1 represents DNA molecular weight marker DL15000, M2 represents DNA molecular weight marker DL5000, and 1-3 represent pC1300-CuLQW1 recombinant expression plasmid;

[0020] Figure 2 It is a pC1300-CuLQW1 overexpression vector structure chart;

[0021] Figure 3 It is a monoclonal colony PCR electrophoresis result chart, in which M represents DNA molecular weight marker DL5000, 1 represents empty load negative control, and 2-4 represent monoclonal colony PCR results;

[0022] Figure 4 It is a PCR detection result chart of transgenic rice plants, in which M represents DNA molecular weight marker DL5000, 1 represents wild type rice negative control, and 2-4 represent transgenic rice plants;

[0023] Figure 5Figure 1 is a phenotype diagram of wild type rice and CuLQW1 overexpression rice strain of the present application, wherein WT represents wild type rice, OE1 and OE2 represent CuLQW1 transgenic rice strain, and the scale is 5 cm;

[0024] Figure 6 Figure 2 is a phenotype and statistical diagram of parenchyma cell thickness of wild type rice and CuLQW1 overexpression rice strain of the present application, wherein A is a phenotype diagram of parenchyma cell thickness of wild type rice, the scale is 40 μm, B is a phenotype diagram of parenchyma cell thickness of CuLQW1 overexpression rice strain OE1, the scale is 40 μm, C is a phenotype diagram of parenchyma cell thickness of CuLQW1 overexpression rice strain OE2, the scale is 40 μm, and D is a statistical diagram of parenchyma cell thickness of wild type rice and CuLQW1 overexpression rice strain, wherein WT represents wild type rice, OE1 and OE2 represent CuLQW1 transgenic rice strain, and " indicates a significant difference level P≤0.01;

[0025] Figure 7 Figure 3 is a diagram of lignin and cellulose content of wild type rice and CuLQW1 transgenic rice strain of the present application, wherein A is lignin content, B is cellulose content, WT represents wild type rice, OE1 and OE2 represent CuLQW1 transgenic rice strain, and " indicates a significant difference level 0.01

[0026] Figure 8 Figure 4 is a growth phenotype diagram of wild type rice and CuLQW1 transgenic rice strain of the present application after normal and drought treatment for 2 weeks, wherein A is a growth phenotype diagram after normal treatment for 2 weeks, B is a growth phenotype diagram after drought treatment for 2 weeks, the scale is 5 cm, WT represents wild type rice, and OE1 and OE2 represent CuLQW1 transgenic rice strain;

[0027] Figure 9 Figure 5 is a biomass analysis diagram of wild type rice and CuLQW1 transgenic rice strain of the present application after normal and drought stress treatment for 2 weeks, wherein A is seedling height growth, B is root length growth, C is aboveground dry weight, D is underground dry weight, E is aboveground fresh weight, and F is underground fresh weight, WT represents wild type rice, OE1 and OE2 represent CuLQW1 transgenic rice strain, "n.s." indicates P>0.05, " indicates a significant difference level 0.01

[0028] Figure 10 ​​​​Figures for photosynthetic and chlorophyll fluorescence parameters of wild type rice and CuLQW1 transgenic lines of the present application after 2 weeks of normal and drought stress treatment, wherein A is net photosynthetic rate, B is stomatal conductance, C is apparent electron transport rate, and D is non-photochemical quenching, and WT represents wild type rice, OE1 and OE2 represent CuLQW1 transgenic lines of rice, "n.s." means P>0.05, " indicates significant level 0.01 " indicates significant level 0.01 " indicates significant level 0.01. DETAILED DESCRIPTION

[0029] Various exemplary embodiments of the present application will now be described in detail, which should be considered to be illustrative of certain aspects, features and embodiments of the present application, but not a limitation thereof.

[0030] It is to be understood that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the present application. Additionally, the use of "including", "comprising", "having", "containing", "involving", and variations thereof herein, is meant to encompass the items listed thereafter and equivalents thereof as well as additional items. Unless otherwise indicated, the use of the terms "a" and "an" are meant to encompass the plural as well as the singular. The use of the term "comprising" is meant to encompass the terms "including", "containing", "having", "involving", and variations thereof. The use of the term "comprising" also is meant to encompass the terms "consisting essentially of" and "consisting of". The use of the term "consisting essentially of" means that the composition can include additional elements not named, but that these additional elements do not materially alter the basic and novel characteristics of the claimed composition. The use of the term "consisting of" means that the composition includes only the elements named.

[0031] Unless otherwise indicated, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which the application pertains. Although methods and materials similar or equivalent to those described herein can be used in the practice or testing of the present application, preferred methods and materials are described. All publications mentioned herein are incorporated by reference to disclose and describe the methods and / or materials in connection with which the publications are cited. In case of conflict between the content of the specification and that of a document incorporated by reference, the content of the specification prevails.

[0032] Various modifications and changes can be made to the specific embodiments of the present application described herein without departing from the scope or spirit of the application. Other embodiments of the application will be apparent to those of ordinary skill in the art from the description and examples presented herein. The description and examples are illustrative of certain aspects, features and embodiments of the present application and are not meant to limit the scope of the application.

[0033] As used herein, the terms "comprise", "comprising", "include", "including", "have", "having" or variants thereof are open-ended, and include one or more steps, integers, compositions or elements of any integer number of steps, integers, compositions or elements. It should be understood that they are not limited to the preferred embodiments.

[0034] The source of the material used in the application: the young shoots of Chimonocalamus praecox collected from the Jinfoshan National Scenic Area in Nanchuan District, Chongqing, at an altitude of 1850 m (28°57'5"N, 107°11'28"E), the pGEM-T easy vector was purchased from Promega (A1360); the E. coli DH5a competent cells were purchased from Beijing Zhuangmeng International Biological Gene Technology Co., Ltd. (ZK206); the PrimerSTAR Mix high-fidelity enzyme amplification enzyme was purchased from Takara (R010Q); the pCAMBIA1300-Ubi-GFP-FLAG (pC1300-Ubi) vector was purchased from the Moliang Plasmid Platform (P29697); the kanamycin resistance (50 mg·L -1 ) was purchased from Beijing Coolab Technology Co., Ltd. (CK6731); the YEP medium was purchased from Beijing Coolab Technology Co., Ltd. (PM0891); the Kimura B rice nutrient solution was purchased from Beijing Coolab Technology Co., Ltd. (NS1050).

[0035] Example 1

[0036] I. Obtain the coding gene of the transcription factor CuLQW1 of Chimonocalamus praecox in Jinfoshan Mountain.

[0037] The young shoots of Chimonocalamus praecox in Jinfoshan Mountain were used as experimental materials, RNA was extracted, and cDNA was reversely transcribed as a template. The predicted gene CHU16g0313 in Chimonocalamus praecox in Jinfoshan Mountain was designed to have specific primers, and the coding region sequence was amplified by PCR.

[0038] The primer sequences are as follows:

[0039] The upstream primer is 5'-AAGCTCCACAAACCTGTCAGC-3' (SEQ ID NO. 3);

[0040] The downstream primer is 5'-GAACCAAAACCCAAGTATCCAA-3' (SEQ ID NO. 4).

[0041] The PCR amplification product was detected by agarose gel electrophoresis, the target band was cut and recovered, the recovered DNA fragment was connected to the pGEM-T easy vector, the E. coli DH5a competent cells were transformed, blue-white spot screening was performed, the positive clone plasmid was extracted and enzyme digestion map analysis was performed, then the single clone was sequenced, and the target fragment of 1284 bp was obtained, as shown in SEQ ID NO. 2.

[0042] Further online comparison and analysis by BlastP software found that the amino acid sequence (as shown in SEQ ID NO. 1) encoded by the coding gene had the highest consistency of 92.09% with the homologous protein (VAH02014.1) of Triticum durum ( Triticum turgidum subsp. durum ), Hordeum vulgareThe homologous protein (KAE8806364.1) of the LQW1 gene has a consistency of 92.07%; protein domain analysis shows that the protein has a MYB family related conserved domain, and has two structures of R2 and R3 at the N terminal, and the structure model is [-W-(X19)-W-(X19)-W-] and [-F-(X18)-W-(X18)-W-]. As can be seen, the cloned gene belongs to R2R3-MYB, and the gene is named as the coding gene of the CuLQW1 transcription factor of the Jinfushan bamboo, and the pGEM-T easy vector containing the gene is pT-CuLQW1.

[0043] The amino acid sequence of the CuLQW1 transcription factor of the Jinfushan bamboo is shown as SEQ ID NO. 1, SEQ ID NO. 1: MGRHSCCYKQKLRKGLWSPEEDEKLMNHITKHGHGCWSSVPKLAGLQRCGKSCRLRWINYLRPDLKRGAFSQEEEDLIIELHTVLGNRWSQIAAQLPGRTDNEIKNLWNSSLKKKLRQRGIDPNTHKPLAEVDRSGAAPTISTERTSGSSDVNPSSAGTLGNLSHLLSETAQSSMLLPVYDKNRPETPSLARPKGPPKELFLDQLPAGHESPSSCRSSGPTLYFPFQQPLGYSNECGSGNGANMNSLWFNQNDFNCSTISTVMPPVSPSALSTSMGLNLPPDNPRHGGTGIGSAPFYWGGANPSSSSSTGSNGSNGMGFEPQCTNSILENSVFPWTDIGQEKDTRVHLVEELKWPDLLHGTFAEATAAMQNQSQSLYDDVIKAESQFNMEGICASWFQNQQPQQQLQAPSDMYDKDLQRMPLSFEHI.

[0044]

[0045] II. Constructing a plant expression vector carrying the coding gene of the transcription factor CuLQW1 from Phyllostachys edulis.

[0046] Based on the coding gene of the transcription factor CuLQW1 from Phyllostachys edulis shown in SEQ ID NO:2, amplification primers with homologous arms and restriction enzyme sites BamHⅠ / PmlⅠ were designed. The obtained correctly sequenced plasmid was diluted 50 times and used as a template. Using PrimerSTAR Mix high-fidelity amplification enzyme, PCR amplification was performed on the fragment containing the restriction enzyme sites (BamHⅠ / PmlⅠ) required for expression vector construction and the coding gene of the transcription factor CuLQW1 from Phyllostachys edulis.

[0047] The primer sequences are as follows:

[0048] Upstream primer: 5′-GTTGTTTGGTGTTACTTCTGCAGggatccATGGGGAGGCATTCTTGTTGC-3′ (SEQ ID NO.5, lowercase letter of BamHI site);

[0049] Downstream primer: 5′-GCCCTTGCTCACCATAGGCCTcacgtgGATATGCTCAAAAGACAAGGGCATTCT-3′ (SEQ ID NO.6, lowercase letter of PmlⅠ site);

[0050] The PCR amplification products were detected by agarose gel electrophoresis, the target band was excised and purified, and the target gene and the enzyme digestion fragment of the pCAMBIA1300-Ubi-GFP-FLAG (pC1300-Ubi) vector were ligated using one-step cloning technology. The reaction system is shown in Table 1 below (10 μL).

[0051] Table 1 Reaction System

[0052]

[0053] Add all reagents from Table 1 to the PCR tube in the order listed in Table 1, mix well, centrifuge, place in a PCR instrument, and incubate at 50°C for 5 min to transform *E. coli* DH5α. Extract plasmids and identify them using restriction enzyme patterns (e.g., ...). Figure 1 After sequencing verification, the obtained recombinant expression vector was named pC1300-CuLQW1 (e.g., ...). Figure 2 ).

[0054] III. Identification of monoclonal colonies containing the expression vector pC1300-CuLQW1.

[0055] Due to rice ( Oryza sativa) and bamboo are monocotyledonous plants of the Gramineae family, and have very close genetic relationship. Therefore, the recombinant expression vector pC1300-CuLQW1 was transformed into Agrobacterium by freeze-thaw method Agrobacterium tumefacien. ) GV3101 competent cells were coated on kanamycin-resistant (50 mg·L -1 ) plates and cultured at 28°C. Single colonies were selected and cultured in YEP medium, and PCR identification was performed on the bacterial solution. The single colonies formed after transformation with the recombinant expression vector were used as templates, and PCR detection was performed using primers with nucleotide sequences as shown in SEQ ID NO. 5 and SEQ ID NO. 6, and the empty vector was used as a negative control.

[0056] As shown in Figure 3 , the PCR electrophoretogram of the Agrobacterium monoclonal colonies transformed with the expression vector pC1300-CuLQW1 plasmid showed that the monoclonal colonies contained the target gene fragment, and the Agrobacterium monoclonal bacterial solution obtained after shaking could be used for infection and transformation experiments.

[0057] Four, transformation of rice by the coding gene of the transcription factor CuLQW1 of the Jinfo Mountain bamboo

[0058] Using the obtained Agrobacterium monoclonal bacterial solution, rice callus was infected by Agrobacterium-mediated method, and through the steps of continuous resistance (50 mg·L -1 ) screening, differentiation and rooting, two rice CuLQW1 transgenic lines OE1 and OE2 were finally obtained, and gene expression detection was performed.

[0059] The total RNA of the rice CuLQW1 transgenic plants (OE1 and OE2) and wild type (WT) plants was extracted and reverse transcribed into cDNA, and the cDNA was used as a template for PCR detection using primers with nucleotide sequences as shown in SEQ ID NO. 5 and SEQ ID NO. 6.

[0060] As shown in Figure 4 , the results showed that the target gene was detected in the rice CuLQW1 transgenic plants (OE1 and OE2), proving that CuLQW1 had been transferred into the rice plants, while no expression was detected in the wild type rice plants (WT). The wild type rice (ET) and the verified transgenic plants (OE1 and OE2) were planted in the field, and T1 generation seeds were collected. After germination, the rice seedlings were watered in a light incubator for two weeks. The light incubator was set at a temperature of 28±2°C, with light for 12 hours and dark for 12 hours, and a relative humidity of 60%. The nutrient solution used for water culture was the Kimura B rice nutrient solution, and the rice seedlings obtained could be used for subsequent experiments (as shown in Figure 5 ).

[0061] Five, analysis of the thickness of the cell wall of the parenchymal cells of the transgenic rice.

[0062] The obtained rice CuLQW1 transgenic seedlings (OE1 and OE2) were cultured to the mature stage, and the stem base was taken for scanning electron microscope observation and thin-walled cell thickness statistics. Analysis found that, as shown in Figure 6 A, Figure 6 B, Figure 6 C and Figure 6 D, the thin-walled cell thickness of the rice CuLQW1 transgenic lines OE1 and OE2 was significantly higher than that of WT, indicating that the transgenic CuLQW1 could thicken the cell wall thickness of the rice stem thin-walled cells.

[0063] Six, transgenic rice lignin and cellulose content analysis.

[0064] From the obtained wild type (WT) and transgenic rice lines (OE1 and OE2) stems at the mature stage, the lignin and cellulose contents were determined by chemical method.

[0065] As shown in Figure 7 A and Figure 7 B, the results showed that compared with the wild type (WT), the lignin content in the rice CuLQW1 transgenic lines OE1 and OE2 increased by 13.85% and 15.08% respectively, and the cellulose increased by 12.96% and 13.90% respectively. In summary, overexpression of the coding gene of the Jinfo Mountain bamboo transcription factor CuLQW1 can increase the content of lignin and cellulose in rice.

[0066] Seven, comparison analysis of transgenic rice biomass under drought stress.

[0067] The obtained wild type rice seedlings (WT) and rice CuLQW1 transgenic seedlings (OE1 and OE2) were drought treated (10% PEG6000) for 2 weeks, and the biomass of the seedlings before and after treatment (such as Figure 8 A and Figure 8 B) was statistically analyzed, including seedling height and root length increment, plant aboveground and underground fresh weight and dry weight, etc.

[0068] Statistical analysis found that, as shown in Figure 9 A, Figure 9 B, Figure 9 C, Figure 9 D, Figure 9 E and Figure 9As shown in Figure F, before drought treatment, there was no significant difference in aboveground and belowground biomass between CuLQW1 transgenic rice seedlings OE1 and OE2 and wild-type rice seedlings (WT). After drought treatment, compared with wild-type rice seedlings (WT), the overexpressing CuLQW1 transgenic lines OE1 and OE2 showed significantly increased seedling height and root length, as well as significantly increased whole-plant dry and fresh biomass. The seedling height increase of CuLQW1 overexpressing transgenic lines OE1 and OE2 was 1.37 times and 1.37 times the root weight (WT) of wild-type rice seedlings, respectively; the root length increase was 1.29 times and 1.25 times the WT, respectively. The aboveground fresh weight and dry weight of CuLQW1 overexpressing transgenic lines OE1 and OE2 were 1.44 times and 1.39 times the WT, respectively, of wild-type rice seedlings, respectively. The underground fresh weight and dry weight of CuLQW1 overexpressing transgenic lines OE1 and OE2 were 1.69 times and 1.70 times the WT, respectively, of wild-type rice seedlings, respectively. These results indicate that CuLQW1 overexpression significantly improves the drought stress tolerance of transgenic rice plants.

[0069] VIII. Comparative analysis of photosynthetic and fluorescence parameters of transgenic rice under drought stress.

[0070] The obtained rice seedlings were subjected to drought treatment. The photosynthetic parameters of the rice seedlings before and after treatment were detected using a portable photosynthesis meter, and the chlorophyll fluorescence parameters were measured using a dual-channel chlorophyll fluorescence meter.

[0071] Statistical analysis found that, for example Figure 10 China A Figure 10 B, Figure 10 C and Figure 10 As shown in Figure D, before drought treatment, there were no significant differences in net photosynthetic rate, stomatal conductance, apparent electron transport rate (ETR), and non-photochemical quenching (NPQ) between the CuLQW1 overexpressing transgenic lines OE1 and OE2. Drought stress significantly reduced the photosynthetic capacity of wild-type rice plants (WT). After drought treatment, the net photosynthetic rate in wild-type rice plants (WT) was 6.6030 µmol / m². 2 / s, porosity is 0.2052 mol / m 2 / sl, ETR is 23.7583μmol electrons / m 2 / s; The net photosynthetic rates of the CuLQW1 transgenic lines OE1 and OE2 were 150.61% (9.9445 µmol / m²) of those of wild-type rice plants (WT). 2 / s) and 157.62% (10.4077µmol / m 2mol / m2s-1) and 191.71% (0.3935 mol / m2s-1), respectively, and ETR were 151.89% (36.0877 μmol electrons / m2s-1) and 152.73% (36.2850 μmol electrons / m2s-1) of wild type rice plants (WT), respectively. It was shown that overexpression of CuLQW1 improved the drought tolerance of transgenic rice. 2 2 2 2 / s)and 152.73%(36.2850μmol electrons / m

[0072] The above-described embodiments are merely preferred modes of the present application, and are not intended to limit the scope of the present application. Various modifications and improvements to the technical solutions of the present application made by those of ordinary skill in the art without departing from the design spirit of the present application shall fall within the scope of protection of the present application as defined by the claims.​​​

Claims

1. A gene encoding the transcription factor CuLQW1 from *Phyllostachys edulis*, characterized in that, The nucleotide sequence of the encoding gene is shown in SEQ ID NO.

2.

2. The transcription factor CuLQW1 encoded by the gene of *Phyllostachys edulis* as described in claim 1, characterized in that, The amino acid sequence of the transcription factor CuLQW1 is shown in SEQ ID NO.

1.

3. A biomaterial comprising the gene encoding as described in claim 1, characterized in that, The biological material is one or more of the following: expression cassette, expression vector, or engineered bacteria.

4. The application of the encoding gene as described in claim 1, the transcription factor CuLQW1 of *Phyllostachys edulis* as described in claim 2, or the biomaterial as described in claim 3 in regulating lignin and cellulose synthesis in gramineous plants, characterized in that... The grass species mentioned is rice.

5. The application according to claim 4, characterized in that, Overexpression of the encoded gene increased the expression level of the transcription factor CuLQW1 in Phyllostachys edulis, thereby increasing the content of lignin and cellulose in the plant.

6. The application of the encoding gene as described in claim 1, the *Phyllostachys edulis* transcription factor CuLQW1 as described in claim 2, or the biomaterial as described in claim 3 in regulating drought stress tolerance in rice, characterized in that... Overexpression of the encoded gene increased the expression level of the transcription factor CuLQW1 in Phyllostachys edulis, thereby improving the drought stress tolerance of rice plants.

7. A method for increasing the lignin and cellulose content in rice, characterized in that, include: Overexpression of the encoding gene described in claim 1 or the transcription factor CuLQW1 of Phyllostachys edulis described in claim 2 in rice increases the lignin and cellulose content in rice.

8. A method for improving the drought stress tolerance of rice, characterized in that, include: Overexpression of the encoding gene described in claim 1 or the transcription factor CuLQW1 of Phyllostachys edulis described in claim 2 in rice can improve the drought stress tolerance of rice plants.

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