Tobacco seedling transplanting efficient growth treatment agent

Abstract

The invention relates to the technical field of crop cultivation and plant protection, in particular to an efficient growth treatment agent for tobacco seedling transplantation. The efficient growth treating agent for tobacco seedling transplanting comprises a group A for treating tobacco seedling leaves and a group B for treating tobacco seedling root systems, and the group A comprises enylalan.oxyenylalani soluble powder and bacillus amyloliquefaciens KN-527; and the group B comprises white spirit of 45 degrees or above, a chlorantraniliprole and indoxacarb suspending agent, olefinic gland and oxyolefinic gland soluble powder, enzymolysis seaweed extract microbial bacteria and bacillus amyloliquefaciens. Through compounding of the composition, the transplanting survival rate and rapid growth of tobacco seedlings can be promoted, diseases and insect pests are killed, and the stress resistance of the tobacco seedlings is improved.

Description

Technical Field

[0001] This invention relates to the field of crop cultivation and plant protection technology, specifically to a high-efficiency growth treatment agent for tobacco seedling transplantation. Background Technology

[0002] Tobacco is an important economic crop in my country, with its planting area remaining stable at around 15 million mu (approximately 1 million hectares) annually. Production areas are widely distributed in provinces such as Yunnan, Guizhou, Sichuan, Hunan, and Hubei. From an agricultural production perspective, rational tobacco planting patterns, such as tobacco-rice rotation and tobacco-vegetable rotation, help improve land utilization. Through alternating crop rotations, they also play a positive role in improving soil structure and maintaining sustainable soil fertility.

[0003] The transplanting stage is the primary stage of tobacco production and the foundation for high-quality tobacco leaves. The management level at this stage directly determines the survival rate, uniformity, and subsequent growth potential of the tobacco plants. Among these factors, the fertilizer and treatment technologies used during transplanting are crucial.

[0004] In the early stages, growers added functional substances during transplanting, such as using a small amount of chemical fertilizer diluted with water as a "transplanting fertilizer," or using traditional soil-based preparations to promote root growth. With the continuous improvement of agricultural technology, the development of biotechnology has also injected new elements into transplanting treatments. Products containing beneficial microorganisms (such as arbuscular mycorrhizal fungi, phosphorus- and potassium-solubilizing bacteria, and biocontrol bacteria) have begun to emerge, aiming to promote healthy tobacco growth by regulating the rhizosphere microecological environment. Despite continuous technological evolution, overall, the current development of tobacco transplanting fertilizers and treatments is still in a transitional phase from traditional to modern methods, and a mature, efficient, and environmentally friendly standardized technical system has not yet been formed.

[0005] Currently, the fertilizer and treatment technologies and their supporting methods widely used in tobacco transplanting practice, while supporting production to some extent, have inherent limitations that have become key bottlenecks restricting further improvements in tobacco yield and quality, and affecting the sustainable development of the industry. These shortcomings of existing technologies manifest themselves at several levels: (i) Existing transplanting treatments often have too few ingredients and efficacy, making it difficult to comprehensively address the complex field environment after transplanting. Most existing transplanting treatments only target a specific problem, such as focusing on rooting or disease and pest resistance, and have limited effect on improving the stress resistance of tobacco seedlings. When tobacco seedlings encounter adverse environments such as drought and high humidity, their resistance is insufficient and the survival rate is reduced.

[0006] (ii) Many treatment agents contain a narrow spectrum of pesticides, often effective only against specific pathogens or pests, making it difficult to address the complex and ever-changing threat of combined pest and disease infestations in tobacco fields. Some treatment agents have a narrow range of pest and disease control, making it difficult to cope with complex and ever-changing pest and disease threats. For example, for tobacco black shank and root-knot nematode disease, single-component treatment agents cannot effectively prevent and control both pests and diseases simultaneously, leading to an increased risk of disease infection in tobacco seedlings and affecting yield and quality.

[0007] (III) Some transplanting treatments have poor environmental compatibility and pose potential risks to the soil ecosystem. Some chemically synthesized agents, especially certain fungicides containing heavy metals or insecticides with high residues, may show good disease inhibition effects in the short term, but they degrade slowly in the soil and leave large amounts of residues with long-term use. This not only directly poisons the beneficial microbial communities in the soil, destroys microbial diversity, and leads to soil microecological imbalance, but may also cause problems such as soil compaction and salinization.

[0008] (iv) Due to the aforementioned incomplete functions, poor effects, and even the risk of phytotoxicity, tobacco seedlings generally exhibit poor stress resistance after transplanting. Poor stress resistance after transplanting: Because the seedling transplanting treatments used are incomplete in function and ineffective, and may even damage young seedlings, they are less conducive to healthy and rapid growth. Weak root systems and poor stress resistance make the seedlings susceptible to pests and diseases later on, and may even lead to stunted growth. This severely impacts tobacco yield.

[0009] (v) Inadequate prevention and control measures during the transplanting stage can easily lead to a chain reaction of risks, such as excessive pesticide residues in tobacco leaves. Due to the poor resistance of tobacco plants, they are prone to tobacco diseases and pests. When diseases and pests occur severely and cause great damage, in order to quickly control diseases and pests and reduce losses, tobacco farmers often use highly toxic chemical pesticides with long-lasting residues for prevention and control, which often leads to the risk of excessive pesticide residues in tobacco leaves.

[0010] (vi) Due to the weak foundation of transplanting, the tobacco plants grow poorly in the later stages and suffer from serious diseases and pests, which will inevitably affect the normal opening of tobacco leaves, the yellowing characteristics in the field, and the final curing quality, resulting in a decline in the yield and grade structure of tobacco leaves, and directly lowering the purchase price of tobacco leaves.

[0011] Furthermore, on the one hand, there are differences in understanding among those skilled in the art; on the other hand, the inventors studied a large number of documents and patents when making this invention, but due to space limitations, not all details and contents were listed in detail. However, this does not mean that the present invention does not possess the features of these prior art. On the contrary, the present invention already possesses all the features of the prior art, and the applicant reserves the right to add relevant prior art to the background art. Summary of the Invention

[0012] This invention relates to the field of crop cultivation and plant protection technology, specifically to a high-efficiency growth treatment agent for tobacco seedling transplantation.

[0013] To address the aforementioned technical problems, one objective of this invention is to provide a highly efficient growth treatment agent for tobacco seedling transplantation, comprising group A for treating tobacco seedling leaves and group B for treating tobacco seedling roots, wherein... Group A contains hydroxyadenine soluble powder and Bacillus amyloliquefaciens KN-527; Group B contains baijiu (Chinese liquor) with an alcohol content of 45 degrees or higher, chlorantraniliprole·indoxacarb suspension, acetamiprid·hydroxyacetamiprid soluble powder, enzymatically hydrolyzed seaweed extract microorganisms, and Bacillus amyloliquefaciens.

[0014] According to a preferred embodiment, each liter of the high-efficiency growth treatment agent A for tobacco seedling transplantation contains, by weight, 0.67-1.33 parts of a 1500-750 times diluted solution of terpinen-hydroxyadenine and 1.67-6.67 parts of a 600-150 times diluted solution of Bacillus amyloliquefaciens KN-527. Preferably, each liter of the high-efficiency growth treatment agent A for tobacco seedling transplantation contains, by weight, 1.6 parts of a 1250 times diluted solution of terpinen-hydroxyadenine and 6.667 parts of a 300 times diluted solution of Bacillus amyloliquefaciens KN-527.

[0015] According to a preferred embodiment, each liter of tobacco seedling transplanting high-efficiency growth treatment agent group B contains, by weight, 0.5-0.8 parts of 2000-1250 times diluted liquor, 0.18-1 part of 5500-1000 times diluted chlorantraniliprole·indoxacarb, 1.33-2.67 parts of 750-375 times diluted ethanoate·hydroxyethanoate, 4-6.9 parts of 250-145 times diluted enzymatic hydrolysate seaweed extract microbial agent, and 3.33-13.33 parts of 300-75 times diluted Bacillus amyloliquefaciens KN-527.

[0016] Preferably, each liter of the high-efficiency growth treatment agent B for tobacco seedling transplantation contains, by weight, 0.57 parts of 1750 times diluted liquor, 0.25 parts of 4000 times diluted chlorantraniliprole·indoxacarb, 1.6 parts of 625 times diluted ethanoate·hydroxyethanoate, 4.65 parts of 215 times diluted enzymatically hydrolyzed seaweed extract microbial agent, and 4.44 parts of 225 times diluted Bacillus amyloliquefaciens KN-527.

[0017] According to a preferred embodiment, the solvent of the high-efficiency growth treatment agent for tobacco seedling transplantation is water.

[0018] According to a preferred embodiment, the high-efficiency growth treatment agent for tobacco seedling transplantation further comprises an adjuvant. Preferably, the adjuvant is a nano-network controlled release agent. More preferably, each liter of the high-efficiency growth treatment agent for tobacco seedling transplantation contains, by weight, 0.1-1 parts of the nano-network controlled release agent.

[0019] Nano-network controlled loss agents can promote the penetration and absorption of active ingredients and control pesticide loss. They can improve pesticide adhesion, prolong pesticide residual effect, control pesticide molecule migration, improve pesticide dispersibility, increase pesticide utilization rate, and enhance efficacy.

[0020] One of the objectives of this invention is to provide a method for transplanting tobacco seedlings, which includes the following steps: Before the tobacco seedlings are transplanted, the leaves are sprayed with Group A of high-efficiency growth treatment agent for tobacco seedling transplanting, which contains soluble powder of hydroxyadenine and Bacillus amyloliquefaciens KN-527. When the tobacco seedlings are transplanted, their roots are soaked in Group B of a high-efficiency growth treatment agent for tobacco seedling transplantation, which contains 45-degree or higher liquor, chlorantraniliprole·indoxacarb suspension, acetamiprid·hydroxyacetamiprid soluble powder, enzymatically hydrolyzed seaweed extract microorganisms and Bacillus amyloliquefaciens.

[0021] According to a preferred embodiment, the spraying time for the high-efficiency growth treatment agent A group for tobacco seedling transplanting is at least 7 days before the seedlings are removed from the greenhouse.

[0022] According to a preferred embodiment, the soaking time of the tobacco seedling transplanting high-efficiency growth treatment agent group B is no more than 1 day before or no more than 1 day after transplanting.

[0023] According to a preferred embodiment, the soaking time for group B of the high-efficiency growth treatment agent for tobacco seedling transplantation is 15-20 minutes. Preferably, the soaking time is 15 minutes. Alternatively, the soaking time is 20 minutes.

[0024] According to a preferred embodiment, the spraying volume of the high-efficiency growth treatment agent A for tobacco seedling transplanting is 50 mL / seedling tray. The seedling tray is a 168-cell seedling tray.

[0025] According to a preferred embodiment, tobacco seedlings soaked in water can be transplanted after being dried until they no longer drip water.

[0026] The components in this invention work synergistically to produce significant beneficial effects. While the liquor exerts its disinfecting and sterilizing effects and cleanses the rhizosphere environment, it also optimizes the absorption state of tobacco seedling roots, significantly improving the penetration and utilization efficiency of other functional components within the seedlings, creating favorable initial conditions for the overall formulation to take effect. Based on this, the functions of each component are interconnected and mutually reinforcing. Chlorantraniliprole·Indoxacarb has broad-spectrum, high-efficiency, fast-acting, long-lasting, contact toxicity, and stomach toxicity, and possesses strong systemic conductivity, allowing it to be transported up and down within the seedlings, providing excellent control over both above-ground and underground pests, thereby reducing the damage caused by pests to tobacco seedlings. Enoxazone·Hydroxyenoxazone stimulates the formation and differentiation of tobacco seedling roots, promotes root growth, encourages the formation of lateral roots, and helps establish and develop the root system. It also regulates the stress resistance of tobacco seedlings, improving their drought and cold resistance, and alleviating damage from diseases, pests, pesticides, fertilizers, and frost. Robust tobacco plants can more efficiently utilize the comprehensive nutrition and biostimulation provided by enzymatically hydrolyzed seaweed extract, further enhancing their vitality. At the same time, Bacillus amyloliquefaciens KN-527 colonizes in the rhizosphere, inhibiting pathogens and improving the soil microenvironment, which not only directly protects the root system but also creates favorable rhizosphere conditions for the continuous and efficient utilization of other components.

[0027] Influenced by the compatibility of physicochemical components, the complementarity rather than mutual exclusion of their mechanisms of action, and the precise matching of their spatiotemporal effects, and verified through the following embodiments, the combined use of these five components can promote the survival rate of transplanted tobacco seedlings, enhance stress resistance, reduce the risk of pesticide residue exceeding standards, and improve the quality of tobacco leaves. The composition of this application exhibits precise coordination in terms of its action level and spatiotemporal sequence, with functional coupling and effect amplification among the components. In terms of spatiotemporal sequence: Baijiu (Chinese liquor) first exerts its unique penetration and disinfection effects, opening absorption channels for subsequent components while also initially "disinfecting" the rhizosphere soil, creating a favorable initial environment for the colonization of beneficial microorganisms. Subsequently, chlorantraniliprole and indoxacarb provide immediate and continuous pest protection, ensuring that tobacco seedlings are protected from biological stress during the most vulnerable early transplanting stage. Under this premise, acetamiprid and hydroxyacetamiprid can efficiently activate the physiological regulatory program of the tobacco seedlings, promoting root development and the establishment of stress resistance mechanisms. Simultaneously, Bacillus amyloliquefaciens KN-527 begins to multiply in the optimized rhizosphere environment, gradually building a stable beneficial microbial community. During this physiological regulation and establishment process, enzymatically hydrolyzed seaweed extract provides continuous nutrition and stress resistance support throughout. Furthermore, the improved root absorption efficiency from the liquor amplifies the regulatory effects of acetylene and hydroxyadenine, as well as the nutritional effects of the enzymatically hydrolyzed seaweed extract. The "insect-free environment" created by chlorantraniliprole and indoxacarb allows tobacco seedlings to allocate more of their physiological resources—originally used for pest defense—to growth and development, thus amplifying the growth-promoting and seedling-strengthening effects of growth regulators and seaweed extract. Meanwhile, the rhizosphere microenvironment improved by Bacillus amyloliquefaciens KN-527 not only directly promotes root health but also enhances the stability and effectiveness of fertilizers and pesticides in the soil. This network of interactive components—creating conditions, enhancing effects, and even conversely reinforcing the effects of other components—is a concept lacking in current compounding technologies.

[0028] In summary, the combination of these ingredients can promote the survival rate and rapid growth of transplanted tobacco seedlings, eliminate pests and diseases, and improve the stress resistance of tobacco seedlings. Detailed Implementation

[0029] In the description of this invention, terminology is used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of indicated technical features. In the description of this invention, "a plurality of" means two or more, unless otherwise explicitly defined.

[0030] Unless otherwise specified, the experimental methods used in the following examples are all conventional methods; the materials, reagents or instruments used, unless otherwise specified by the manufacturer, are all commercially available reagents and materials; the conditions not specified in the examples are all carried out according to conventional conditions or conditions recommended by the manufacturer. At the same time, the present invention does not limit the source of the raw materials used. Unless otherwise specified, the raw materials used in the present invention are all commercially available products in this technical field.

[0031] In the following examples, the water-soluble fertilizer containing macro-elements is a balanced water-soluble fertilizer (N:P:K=17:17:17).

[0032] In the following examples, the nano-network controlled release agent is a nano-network pesticide controlled release agent that uses nanomaterials to construct an interfacial network structure, which captures pesticide molecules and water through microscopic forces, reduces pesticide volatilization and loss, and improves pesticide utilization.

[0033] In the following examples, "specific dilution ratio" means a spray diluted with one part of the agent and the corresponding number of parts of water. For example, "1500 dilution of alkenyl-hydroxyl-acid soluble powder" means a solution formed by diluting 1 part alkenyl-hydroxyl-acid soluble powder with 1500 parts of water by weight.

[0034] Example 1 1. Formula for tobacco seedling transplanting treatment agent 0.004% Aldenyl Aldenyl Soluble Powder 1500 times dilution, 10 billion CFU / mL Bacillus amyloliquefaciens KN-527 Suspension 600 times dilution, Nano-Network Controlled Loss Agent 2000 times dilution (Zhongke Hehui Nano-Network Pesticide Loss Agent SC108).

[0035] 2. Processing steps (1) Measure 2 L of clean water and pour it into a container. Following the pesticide preparation order, add 13.33 g of 0.004% adenine·hydroxyadenine soluble powder, 33.33 g of 10 billion CFU / mL Bacillus amyloliquefaciens KN-527 suspension concentrate, and 10 g of nano-network structure control agent to obtain the mother liquor. During the preparation process, the next pesticide can only be added after the previous pesticide has completely dissolved and been stirred evenly.

[0036] (2) Pour the prepared stock solution into the sprayer, add water to 20 L, stir well, and spray the tobacco seedlings evenly.

[0037] Example 2 1. Formula for tobacco seedling transplanting treatment agent 0.004% hydroxyl adenosine soluble powder at 1500 times dilution, 8000 IU / µL Bacillus thuringiensis suspension at 400 times dilution, and nano-network structure control agent at 2000 times dilution.

[0038] 2. Processing steps (1) Measure 2 L of clean water and pour it into a container. Following the pesticide preparation order, add 13.33 g of 0.004% hydroxyl adenine soluble powder, 50 g of 8000 IU / µL Bacillus thuringiensis suspension, and 10 g of nano-mesh release control agent to obtain the mother liquor. During the preparation process, the next pesticide can only be added after the previous pesticide has completely dissolved and been stirred evenly.

[0039] (2) Pour the prepared stock solution into the sprayer, add water to 20 L, stir well, and spray the tobacco seedlings evenly.

[0040] Example 3 1. Formula for tobacco seedling transplanting treatment agent 0.0016% 28-epibrassinolide aqueous solution 1000 times dilution, 10 billion CFU / mL Bacillus amyloliquefaciens KN-527 suspension 600 times dilution, nano-network structure control agent 2000 times dilution.

[0041] 2. Processing steps (1) Measure 2 L of clean water and pour it into a container. Following the pesticide preparation order, add 20 g of 0.0016% 28-epibrassinolide aqueous solution, 33.33 g of 10 billion CFU / mL Bacillus amyloliquefaciens KN-527 suspension concentrate, and 10 g of nano-network structure control agent to obtain the mother liquor. During the preparation process, the next pesticide can only be added after the previous pesticide has completely dissolved and been stirred evenly.

[0042] (2) Pour the prepared stock solution into the sprayer, add water to 20 L, stir well, and spray the tobacco seedlings evenly.

[0043] Example 4 1. Formula for tobacco seedling transplanting treatment agent 0.0016% 28-epiglosporin lactone aqueous solution 1000 times dilution, 8000 IU / µL Bacillus thuringiensis suspension 400 times dilution, nano-network structure control agent 2000 times dilution.

[0044] 2. Processing steps (1) Measure 2 L of clean water and pour it into a container. Following the pesticide preparation order, add 20 g of 0.0016% 28-epibrassinolide aqueous solution, 50 g of 8000 IU / µL Bacillus thuringiensis suspension, and 10 g of nano-network release control agent to obtain the mother liquor. During the preparation process, the next pesticide can only be added after the previous pesticide has completely dissolved and been stirred evenly.

[0045] (2) Pour the prepared stock solution into the sprayer, add water to 20 L, stir well, and spray the tobacco seedlings evenly.

[0046] Example 5 1. Formula for tobacco seedling transplanting treatment agent 0.004% adenine-hydroxyadenine soluble powder at 750 times dilution, 10 billion CFU / mL Bacillus amyloliquefaciens KN-527 suspension at 150 times dilution, and nano-network structure control agent at 2000 times dilution.

[0047] 2. Processing steps (1) Measure 2 L of clean water and pour it into a container. Following the pesticide preparation order, add 26.67 g of 0.004% adenine·hydroxyadenine soluble powder, 133.33 g of 10 billion CFU / mL Bacillus amyloliquefaciens KN-527 suspension concentrate, and 10 g of nano-network structure control agent to obtain the mother liquor. During the preparation process, the next pesticide can only be added after the previous pesticide has completely dissolved and been stirred evenly.

[0048] (2) Pour the prepared stock solution into the sprayer, add water to 20 L, stir well, and spray the tobacco seedlings evenly.

[0049] Example 6 1. Formula for tobacco seedling transplanting treatment agent 0.004% adenine-hydroxyadenine soluble powder at 750 times dilution, 10 billion CFU / mL Bacillus amyloliquefaciens KN-527 suspension at 300 times dilution, and nano-network structure control agent at 2000 times dilution.

[0050] 2. Processing steps (1) Measure 2 L of clean water and pour it into a container. Following the pesticide preparation order, add 26.67 g of 0.004% adenine·hydroxyadenine soluble powder, 66.67 g of 10 billion CFU / mL Bacillus amyloliquefaciens KN-527 suspension concentrate, and 10 g of nano-network structure control agent to obtain the mother liquor. During the preparation process, the next pesticide can only be added after the previous pesticide has completely dissolved and been stirred evenly.

[0051] (2) Pour the prepared stock solution into the sprayer, add water to 20 L, stir well, and spray the tobacco seedlings evenly.

[0052] Example 7 1. Formula for tobacco seedling transplanting treatment agent 0.004% adenine-hydroxyadenine soluble powder 750 times dilution, 10 billion CFU / mL Bacillus amyloliquefaciens KN-527 suspension 450 times dilution, nano-network structure control agent 2000 times dilution.

[0053] 2. Processing steps (1) Measure 2 L of clean water and pour it into a container. Following the pesticide preparation order, add 26.67 g of 0.004% adenine·hydroxyadenine soluble powder, 44.44 g of 10 billion CFU / mL Bacillus amyloliquefaciens KN-527 suspension concentrate, and 10 g of nano-network structure control agent to obtain the mother liquor. During the preparation process, the next pesticide can only be added after the previous pesticide has completely dissolved and been stirred evenly.

[0054] (2) Pour the prepared stock solution into the sprayer, add water to 20 L, stir well, and spray the tobacco seedlings evenly.

[0055] Example 8 1. Formula for tobacco seedling transplanting treatment agent 0.004% adenine-hydroxyadenine soluble powder at 750 times dilution, 10 billion CFU / mL Bacillus amyloliquefaciens KN-527 suspension at 600 times dilution, and nano-network structure control agent at 2000 times dilution.

[0056] 2. Processing steps (1) Measure 2 L of clean water and pour it into a container. Following the pesticide preparation order, add 26.67 g of 0.004% adenine·hydroxyadenine soluble powder, 33.33 g of 10 billion CFU / mL Bacillus amyloliquefaciens KN-527 suspension concentrate, and 10 g of nano-mesh release control agent to obtain the mother liquor. During the preparation process, the next pesticide can only be added after the previous pesticide has completely dissolved and been stirred evenly.

[0057] (2) Pour the prepared stock solution into the sprayer, add water to 20 L, stir well, and spray the tobacco seedlings evenly.

[0058] Example 9 1. Formula for tobacco seedling transplanting treatment agent 0.004% adenine·hydroxyadenine soluble powder at 1000 times dilution, 10 billion CFU / mL Bacillus amyloliquefaciens KN-527 suspension at 150 times dilution, and nano-network structure control agent at 2000 times dilution.

[0059] 2. Processing steps (1) Measure 2 L of clean water and pour it into a container. Following the pesticide preparation order, add 20 g of 0.004% adenine·hydroxyadenine soluble powder, 133.33 g of 10 billion CFU / mL Bacillus amyloliquefaciens KN-527 suspension concentrate, and 10 g of nano-mesh release control agent to obtain the mother liquor. During the preparation process, the next pesticide can only be added after the previous pesticide has completely dissolved and been stirred evenly.

[0060] (2) Pour the prepared stock solution into the sprayer, add water to 20 L, stir well, and spray the tobacco seedlings evenly.

[0061] Example 10 1. Formula for tobacco seedling transplanting treatment agent 0.004% adenine·hydroxyadenine soluble powder at 1000x dilution, 10 billion CFU / mL Bacillus amyloliquefaciens KN-527 suspension at 300x dilution, and nano-network structure control agent at 2000x dilution.

[0062] 2. Processing steps (1) Measure 2 L of clean water and pour it into a container. Following the pesticide preparation order, add 20 g of 0.004% adenine·hydroxyadenine soluble powder, 66.67 g of 10 billion CFU / mL Bacillus amyloliquefaciens KN-527 suspension concentrate, and 10 g of nano-network structure control agent to obtain the mother liquor. During the preparation process, the next pesticide can only be added after the previous pesticide has completely dissolved and been stirred evenly.

[0063] (2) Pour the prepared stock solution into the sprayer, add water to 20 L, stir well, and spray the tobacco seedlings evenly.

[0064] Example 11 1. Formula for tobacco seedling transplanting treatment agent 0.004% adenine-hydroxyadenine soluble powder at 1000x dilution, 10 billion CFU / mL Bacillus amyloliquefaciens KN-527 suspension at 450x dilution, and nano-network structure control agent at 2000x dilution.

[0065] 2. Processing steps (1) Measure 2 L of clean water and pour it into a container. Following the pesticide preparation order, add 20 g of 0.004% adenine·hydroxyadenine soluble powder, 44.44 g of 10 billion CFU / mL Bacillus amyloliquefaciens KN-527 suspension concentrate, and 10 g of nano-network structure control agent to obtain the mother liquor. During the preparation process, the next pesticide can only be added after the previous pesticide has completely dissolved and been stirred evenly.

[0066] (2) Pour the prepared stock solution into the sprayer, add water to 20 L, stir well, and spray the tobacco seedlings evenly.

[0067] Example 12 1. Formula for tobacco seedling transplanting treatment agent 0.004% adenine·hydroxyadenine soluble powder at 1000x dilution, 10 billion CFU / mL Bacillus amyloliquefaciens KN-527 suspension at 600x dilution, and nano-network structure control agent at 2000x dilution.

[0068] 2. Processing steps (1) Measure 2 L of clean water and pour it into a container. Following the pesticide preparation order, add 20 g of 0.004% adenine·hydroxyadenine soluble powder, 33.33 g of 10 billion CFU / mL Bacillus amyloliquefaciens KN-527 suspension concentrate, and 10 g of nano-network structure control agent to obtain the mother liquor. During the preparation process, the next pesticide can only be added after the previous pesticide has completely dissolved and been stirred evenly.

[0069] (2) Pour the prepared stock solution into the sprayer, add water to 20 L, stir well, and spray the tobacco seedlings evenly.

[0070] Example 13 1. Formula for tobacco seedling transplanting treatment agent 0.004% adenine·hydroxyadenine soluble powder at 1250 times dilution, 10 billion CFU / mL Bacillus amyloliquefaciens KN-527 suspension at 150 times dilution, and nano-network structure control agent at 2000 times dilution.

[0071] 2. Processing steps (1) Measure 2 L of clean water and pour it into a container. Following the pesticide preparation order, add 16 g of 0.004% adenine·hydroxyadenine soluble powder, 133.33 g of 10 billion CFU / mL Bacillus amyloliquefaciens KN-527 suspension concentrate, and 10 g of nano-mesh release control agent to obtain the mother liquor. During the preparation process, the next pesticide can only be added after the previous pesticide has completely dissolved and been stirred evenly.

[0072] (2) Pour the prepared stock solution into the sprayer, add water to 20 L, stir well, and spray the tobacco seedlings evenly.

[0073] Example 14 1. Formula for tobacco seedling transplanting treatment agent 0.004% adenine·hydroxyadenine soluble powder at 1250 times dilution, 10 billion CFU / mL Bacillus amyloliquefaciens KN-527 suspension at 300 times dilution, and nano-network structure control agent at 2000 times dilution.

[0074] 2. Processing steps (1) Measure 2 L of clean water and pour it into a container. Following the pesticide preparation order, add 16 g of 0.004% adenine·hydroxyadenine soluble powder, 66.67 g of 10 billion CFU / mL Bacillus amyloliquefaciens KN-527 suspension concentrate, and 10 g of nano-network structure control agent to obtain the mother liquor. During the preparation process, the next pesticide can only be added after the previous pesticide has completely dissolved and been stirred evenly.

[0075] (2) Pour the prepared stock solution into the sprayer, add water to 20 L, stir well, and spray the tobacco seedlings evenly.

[0076] Example 15 1. Formula for tobacco seedling transplanting treatment agent 0.004% adenine-hydroxyadenine soluble powder at 1250 times dilution, 10 billion CFU / mL Bacillus amyloliquefaciens KN-527 suspension at 450 times dilution, and nano-network structure control agent at 2000 times dilution.

[0077] 2. Processing steps (1) Measure 2 L of clean water and pour it into a container. Following the pesticide preparation order, add 16 g of 0.004% adenine·hydroxyadenine soluble powder, 44.44 g of 10 billion CFU / mL Bacillus amyloliquefaciens KN-527 suspension concentrate, and 10 g of nano-network structure control agent to obtain the mother liquor. During the preparation process, the next pesticide can only be added after the previous pesticide has completely dissolved and been stirred evenly.

[0078] (2) Pour the prepared stock solution into the sprayer, add water to 20 L, stir well, and spray the tobacco seedlings evenly.

[0079] Example 16 1. Formula for tobacco seedling transplanting treatment agent 0.004% adenine-hydroxyadenine soluble powder at 1250 times dilution, 10 billion CFU / mL Bacillus amyloliquefaciens KN-527 suspension at 600 times dilution, and nano-network structure control agent at 2000 times dilution.

[0080] 2. Processing steps (1) Measure 2 L of clean water and pour it into a container. Following the pesticide preparation order, add 16 g of 0.004% adenine·hydroxyadenine soluble powder, 33.33 g of 10 billion CFU / mL Bacillus amyloliquefaciens KN-527 suspension concentrate, and 10 g of nano-mesh release control agent to obtain the mother liquor. During the preparation process, the next pesticide can only be added after the previous pesticide has completely dissolved and been stirred evenly.

[0081] (2) Pour the prepared stock solution into the sprayer, add water to 20 L, stir well, and spray the tobacco seedlings evenly.

[0082] Example 17 1. Formula for tobacco seedling transplanting treatment agent 0.004% adenine·hydroxyadenine soluble powder at 1500 times dilution, 10 billion CFU / mL Bacillus amyloliquefaciens KN-527 suspension at 150 times dilution, and nano-network structure control agent at 2000 times dilution.

[0083] 2. Processing steps (1) Measure 2 L of clean water and pour it into a container. Following the pesticide preparation order, add 13.33 g of 0.004% adenine·hydroxyadenine soluble powder, 133.33 g of 10 billion CFU / mL Bacillus amyloliquefaciens KN-527 suspension concentrate, and 10 g of nano-network structure control agent to obtain the mother liquor. During the preparation process, the next pesticide can only be added after the previous pesticide has completely dissolved and been stirred evenly.

[0084] (2) Pour the prepared stock solution into the sprayer, add water to 20 L, stir well, and spray the tobacco seedlings evenly.

[0085] Example 18 1. Formula for tobacco seedling transplanting treatment agent 0.004% adenine·hydroxyadenine soluble powder at 1500 times dilution, 10 billion CFU / mL Bacillus amyloliquefaciens KN-527 suspension at 300 times dilution, and nano-network structure control agent at 2000 times dilution.

[0086] 2. Processing steps (1) Measure 2 L of clean water and pour it into a container. Following the pesticide preparation order, add 13.33 g of 0.004% adenine·hydroxyadenine soluble powder, 66.67 g of 10 billion CFU / mL Bacillus amyloliquefaciens KN-527 suspension concentrate, and 10 g of nano-network structure control agent to obtain the mother liquor. During the preparation process, the next pesticide can only be added after the previous pesticide has completely dissolved and been stirred evenly.

[0087] (2) Pour the prepared stock solution into the sprayer, add water to 20 L, stir well, and spray the tobacco seedlings evenly.

[0088] Example 19 1. Formula for tobacco seedling transplanting treatment agent 0.004% adenine·hydroxyadenine soluble powder at 1500 times dilution, 10 billion CFU / mL Bacillus amyloliquefaciens KN-527 suspension at 450 times dilution, and nano-network structure control agent at 2000 times dilution.

[0089] 2. Processing steps (1) Measure 2 L of clean water and pour it into a container. Following the pesticide preparation order, add 13.33 g of 0.004% adenine·hydroxyadenine soluble powder, 44.44 g of 10 billion CFU / mL Bacillus amyloliquefaciens KN-527 suspension concentrate, and 10 g of nano-network structure control agent to obtain the mother liquor. During the preparation process, the next pesticide can only be added after the previous pesticide has completely dissolved and been stirred evenly.

[0090] (2) Pour the prepared stock solution into the sprayer, add water to 20 L, stir well, and spray the tobacco seedlings evenly.

[0091] Example 20 1. Formula for tobacco seedling transplanting treatment agent 0.004% adenine·hydroxyadenine soluble powder at 1500 times dilution, 10 billion CFU / mL Bacillus amyloliquefaciens KN-527 suspension at 600 times dilution, and nano-network structure control agent at 2000 times dilution.

[0092] 2. Processing steps (1) Measure 2 L of clean water and pour it into a container. Following the pesticide preparation order, add 13.33 g of 0.004% adenine·hydroxyadenine soluble powder, 33.33 g of 10 billion CFU / mL Bacillus amyloliquefaciens KN-527 suspension concentrate, and 10 g of nano-network structure control agent to obtain the mother liquor. During the preparation process, the next pesticide can only be added after the previous pesticide has completely dissolved and been stirred evenly.

[0093] (2) Pour the prepared stock solution into the sprayer, add water to 20 L, stir well, and spray the tobacco seedlings evenly.

[0094] Example 21 1. Formula for tobacco seedling transplanting treatment agent Red wine 2000 times dilution, 25% chlorantraniliprole·indoxacarb suspension 5500 times dilution, 0.004% acetamiprid·hydroxyacetamiprid soluble powder 750 times dilution, enzymatic hydrolysis of seaweed extract microbial agent 250 times dilution, 10 billion CFU / mL Bacillus amyloliquefaciens KN-527 suspension 300 times dilution, nano-network structure control agent 2000 times dilution.

[0095] 2. Processing steps (1) Measure 2 L of clean water and pour it into a container. Following the order of pesticide preparation, add 80 g of enzymatically hydrolyzed seaweed extract microbial agent, 26.67 g of 0.004% acetamiprid·hydroxyacetamiprid soluble powder, 3.64 g of 25% chlorantraniliprole·indoxacarb suspension, 67.67 g of 10 billion CFU / mL Bacillus amyloliquefaciens KN-527 suspension, 10 g of red wine, and 10 g of nano-mesh release control agent to obtain the mother liquor. During the preparation process, the next agent can only be added after the previous agent has completely dissolved and been stirred evenly.

[0096] (2) Pour the prepared mother liquor into a container, add water to 20 L, stir well, and immerse the roots of the tobacco seedlings in the solution for 15-20 minutes.

[0097] Example 22 1. Formula for tobacco seedling transplanting treatment agent Red wine 2000x dilution, 25% chlorantraniliprole·indoxacarb suspension 5500x dilution, 0.0016% 28-epi-hypobrachiin lactone aqueous solution 500x dilution, enzymatic hydrolysis of seaweed extract microbial agent 250x dilution, 10 billion CFU / mL Bacillus amyloliquefaciens KN-527 suspension 300x dilution, nano-network structure control agent 2000x dilution.

[0098] 2. Processing steps (1) Measure 2 L of clean water and pour it into a container. Following the order of pesticide preparation, add 80 g of enzymatically hydrolyzed seaweed extract microbial agent, 40 g of 0.0016% 28-epibrassinolide aqueous solution, 3.64 g of 25% chlorantraniliprole·indoxacarb suspension, 67.67 g of 10 billion CFU / mL Bacillus amyloliquefaciens KN-527 suspension, 10 g of red wine, and 10 g of nano-mesh release control agent to obtain the mother liquor. During the preparation process, the next agent can only be added after the previous agent has completely dissolved and been stirred evenly.

[0099] (2) Pour the prepared mother liquor into a container, add water to 20 L, stir well, and immerse the roots of the tobacco seedlings in the solution for 15-20 minutes.

[0100] Example 23 1. Formula for tobacco seedling transplanting treatment agent Red wine 2000 times dilution, 25% chlorantraniliprole·indoxacarb suspension 5500 times dilution, 0.004% acetamiprid·hydroxyacetamiprid soluble powder 750 times dilution, water-soluble fertilizer containing macro-elements 500 times dilution, 10 billion CFU / mL Bacillus amyloliquefaciens KN-527 suspension 300 times dilution, nano-network structure control release agent 2000 times dilution.

[0101] 2. Processing steps (1) Measure 2 L of clean water and pour it into a container. Following the order of pesticide preparation, add 40 g of water-soluble fertilizer containing macro-elements, 26.67 g of 0.004% acetamiprid·hydroxyacetamiprid soluble powder, 3.64 g of 25% chlorantraniliprole·indoxacarb suspension, 67.67 g of 10 billion CFU / mL Bacillus amyloliquefaciens KN-527 suspension, 10 g of red wine, and 10 g of nano-mesh release control agent to obtain the mother liquor. During the preparation process, the next pesticide can only be added after the previous pesticide has completely dissolved and been stirred evenly.

[0102] (2) Pour the prepared mother liquor into a container, add water to 20 L, stir well, and immerse the roots of the tobacco seedlings in the solution for 15-20 minutes.

[0103] Example 24 1. Formula for tobacco seedling transplanting treatment agent Red wine 2000 times dilution, 25% chlorantraniliprole·indoxacarb suspension 5500 times dilution, 0.0016% 28-epiobrassinolide aqueous solution 500 times dilution, macro-element water-soluble fertilizer 500 times dilution, 10 billion CFU / mL Bacillus amyloliquefaciens KN-527 suspension 300 times dilution, nano-network structure control release agent 2000 times dilution.

[0104] 2. Processing steps (1) Measure 2 L of clean water and pour it into a container. Following the order of pesticide preparation, add 40 g of water-soluble fertilizer containing macro-elements, 40 g of 0.0016% 28-epibrinolytic lactone aqueous solution, 3.64 g of 25% chlorantraniliprole·indoxacarb suspension, 67.67 g of 10 billion CFU / mL Bacillus amyloliquefaciens KN-527 suspension, 10 g of red wine, and 10 g of nano-network structure control agent to obtain the mother liquor. During the preparation process, the next pesticide can only be added after the previous pesticide has completely dissolved and been stirred evenly.

[0105] (2) Pour the prepared mother liquor into a container, add water to 20 L, stir well, and immerse the roots of the tobacco seedlings in the solution for 15-20 minutes.

[0106] Example 25 1. Formula for tobacco seedling transplanting treatment agent Red wine 2000 times dilution, 25% chlorantraniliprole·indoxacarb suspension 5500 times dilution, 0.004% acetamiprid·hydroxyacetamiprid soluble powder 750 times dilution, enzymatically hydrolyzed seaweed extract microbial agent 250 times dilution, 8000 IU / µL Bacillus thuringiensis suspension 200 times dilution, nano-network structure control agent 2000 times dilution.

[0107] 2. Processing steps (1) Measure 2 L of clean water and pour it into a container. Following the pesticide preparation order, add 80 g of enzymatically hydrolyzed seaweed microbial agent, 26.67 g of 0.004% acetamiprid·hydroxyacetamiprid soluble powder, 3.64 g of 25% chlorantraniliprole·indoxacarb suspension, 100 g of 8000 IU / µL Bacillus thuringiensis suspension, 10 g of red wine, and 10 g of nano-mesh release control agent to obtain the mother liquor. During the preparation process, the next pesticide can only be added after the previous pesticide has completely dissolved and been stirred evenly.

[0108] (2) Pour the prepared mother liquor into a container, add water to 20 L, stir well, and immerse the roots of the tobacco seedlings in the solution for 15-20 minutes.

[0109] Example 26 1. Formula for tobacco seedling transplanting treatment agent Red wine 2000 times dilution, 25% chlorantraniliprole·indoxacarb suspension 5500 times dilution, 0.0016% 28-epi-hypobrachiin lactone aqueous solution 500 times dilution, enzymatic hydrolysis seaweed extract microbial agent 250 times dilution, 8000 IU / µL Bacillus thuringiensis suspension 200 times dilution, nano-network structure control agent 2000 times dilution.

[0110] 2. Processing steps (1) Measure 2 L of clean water and pour it into a container. Following the order of pesticide preparation, add 80 g of enzymatically hydrolyzed seaweed extract microbial agent, 40 g of 0.0016% 28-epibrassinolide aqueous solution, 3.64 g of 25% chlorantraniliprole·indoxacarb suspension, 100 g of 8000 IU / µL Bacillus thuringiensis suspension, 10 g of red wine, and 10 g of nano-mesh release control agent to obtain the mother liquor. During the preparation process, the next pesticide can only be added after the previous pesticide has completely dissolved and been stirred evenly.

[0111] (2) Pour the prepared mother liquor into a container, add water to 20 L, stir well, and immerse the roots of the tobacco seedlings in the solution for 15-20 minutes.

[0112] Example 27 1. Formula for tobacco seedling transplanting treatment agent Red wine 2000 times dilution, 25% chlorantraniliprole·indoxacarb suspension 5500 times dilution, 0.004% acetamiprid·hydroxyacetamiprid soluble powder 750 times dilution, water-soluble fertilizer containing macro-elements 500 times dilution, 8000 IU / µL Bacillus thuringiensis suspension 200 times dilution, nano-network structure control agent 2000 times dilution.

[0113] 2. Processing steps (1) Measure 2 L of clean water and pour it into a container. Following the order of pesticide preparation, add 40 g of water-soluble fertilizer containing macro-elements, 26.67 g of 0.004% acetamiprid·hydroxyacetamiprid soluble powder, 3.64 g of 25% chlorantraniliprole·indoxacarb suspension, 100 g of 8000 IU / µL Bacillus thuringiensis suspension, 10 g of red wine, and 10 g of nano-mesh release control agent to obtain the mother liquor. During the preparation process, the next pesticide can only be added after the previous pesticide has completely dissolved and been stirred evenly.

[0114] (2) Pour the prepared mother liquor into a container, add water to 20 L, stir well, and immerse the roots of the tobacco seedlings in the solution for 15-20 minutes.

[0115] Example 28 1. Formula for tobacco seedling transplanting treatment agent Red wine 2000 times dilution, 25% chlorantraniliprole·indoxacarb suspension 5500 times dilution, 0.0016% 28-epi-hypobrachiin lactone aqueous solution 500 times dilution, macro-element water-soluble fertilizer 500 times dilution, 8000 IU / µL Bacillus thuringiensis suspension 200 times dilution, nano-network structure control agent 2000 times dilution.

[0116] 2. Processing steps (1) Measure 2 L of clean water and pour it into a container. Following the order of pesticide preparation, add 40 g of water-soluble fertilizer containing macro-elements, 40 g of 0.0016% 28-epibrassinolide aqueous solution, 3.64 g of 25% chlorantraniliprole·indoxacarb suspension, 100 g of 8000 IU / µL Bacillus thuringiensis suspension, 10 g of red wine, and 10 g of nano-network release control agent to obtain the mother liquor. During the preparation process, the next pesticide can only be added after the previous pesticide has completely dissolved and been stirred evenly.

[0117] (2) Pour the prepared mother liquor into a container, add water to 20 L, stir well, and immerse the roots of the tobacco seedlings in the solution for 15-20 minutes.

[0118] Example 29 1. Formula for tobacco seedling transplanting treatment agent Red wine (2000x dilution), 25 g / L high-efficiency cyhalothrin emulsifiable concentrate (2000x dilution), 0.004% acetylene-hydroxyacetylene soluble powder (750x dilution), enzymatically hydrolyzed seaweed extract microbial inoculant (250x dilution), 10 billion CFU / mL Bacillus amyloliquefaciens KN-527 suspension concentrate (300x dilution), and nano-network structure control agent (2000x dilution).

[0119] 2. Processing steps (1) Measure 2 L of clean water and pour it into a container. Following the order of pesticide preparation, add 80 g of enzymatically hydrolyzed seaweed extract microbial agent, 26.67 g of 0.004% acetamiprid·hydroxyadenine soluble powder, 10 g of 25 g / L high-efficiency cyhalothrin EC, 67.67 g of 10 billion CFU / mL Bacillus amyloliquefaciens KN-527 suspension concentrate, 10 g of red wine, and 10 g of nano-network structure control agent to obtain the mother liquor. During the preparation process, the next agent can only be added after the previous agent has completely dissolved and been stirred evenly.

[0120] (2) Pour the prepared mother liquor into a container, add water to 20 L, stir well, and immerse the roots of the tobacco seedlings in the solution for 15-20 minutes.

[0121] Example 30 1. Formula for tobacco seedling transplanting treatment agent Red wine (2000x dilution), 25 g / L high-efficiency cyhalothrin emulsifiable concentrate (2000x dilution), 0.0016% 28-epi-hypobrachiin lactone aqueous solution (500x dilution), enzymatically hydrolyzed seaweed extract microbial inoculant (250x dilution), 10 billion CFU / mL Bacillus amyloliquefaciens KN-527 suspension concentrate (300x dilution), and nano-network structure control agent (2000x dilution).

[0122] 2. Processing steps (1) Measure 2 L of clean water and pour it into a container. Following the order of pesticide preparation, add 80 g of enzymatically hydrolyzed seaweed extract microbial agent, 40 g of 0.0016% 28-epibrassinolide aqueous solution, 10 g of 25 g / L high-efficiency cyhalothrin emulsifiable concentrate, 67.67 g of 10 billion CFU / mL Bacillus amyloliquefaciens KN-527 suspension concentrate, 10 g of red wine, and 10 g of nano-network structure control agent to obtain the mother liquor. During the preparation process, the next agent can only be added after the previous agent has completely dissolved and been stirred evenly.

[0123] (2) Pour the prepared mother liquor into a container, add water to 20 L, stir well, and immerse the roots of the tobacco seedlings in the solution for 15-20 minutes.

[0124] Example 31 1. Formula for tobacco seedling transplanting treatment agent Red wine (2000x dilution), 25 g / L high-efficiency cyhalothrin emulsifiable concentrate (2000x dilution), 0.004% acetylene·hydroxyacetylene soluble powder (750x dilution), water-soluble fertilizer containing macro-elements (500x dilution), Bacillus amyloliquefaciens KN-527 suspension concentrate (10 billion CFU / mL) (300x dilution), and nano-network structure control release agent (2000x dilution).

[0125] 2. Processing steps (1) Measure 2 L of clean water and pour it into a container. Following the order of pesticide preparation, add 40 g of water-soluble fertilizer containing macro-elements, 26.67 g of 0.004% acetamiprid·hydroxyadenine soluble powder, 10 g of 25 g / L high-efficiency cyhalothrin EC, 67.67 g of 10 billion CFU / mL Bacillus amyloliquefaciens KN-527 suspending agent, 10 g of red wine, and 10 g of nano-network structure control agent to obtain the mother liquor. During the preparation process, the next pesticide can only be added after the previous pesticide has completely dissolved and been stirred evenly.

[0126] (2) Pour the prepared mother liquor into a container, add water to 20 L, stir well, and immerse the roots of the tobacco seedlings in the solution for 15-20 minutes.

[0127] Example 32 1. Formula for tobacco seedling transplanting treatment agent Red wine (2000x dilution), 25 g / L high-efficiency cyhalothrin emulsifiable concentrate (2000x dilution), 0.0016% 28-epi-hypobrachiin lactone aqueous solution (500x dilution), water-soluble fertilizer containing macro-elements (500x dilution), Bacillus amyloliquefaciens KN-527 suspension concentrate (10 billion CFU / mL) (300x dilution), and nano-network structure control release agent (2000x dilution).

[0128] 2. Processing steps (1) Measure 2 L of clean water and pour it into a container. Following the order of pesticide preparation, add 40 g of water-soluble fertilizer containing macro-elements, 40 g of 0.0016% 28-epibrassinolide aqueous solution, 10 g of 25 g / L high-efficiency cyhalothrin emulsifiable concentrate, 67.67 g of 10 billion CFU / mL Bacillus amyloliquefaciens KN-527 suspension concentrate, 10 g of red wine, and 10 g of nano-network structure control agent to obtain the mother liquor. During the preparation process, the next pesticide can only be added after the previous pesticide has completely dissolved and been stirred evenly.

[0129] (2) Pour the prepared mother liquor into a container, add water to 20 L, stir well, and immerse the roots of the tobacco seedlings in the solution for 15-20 minutes.

[0130] Example 33 1. Formula for tobacco seedling transplanting treatment agent Red wine (2000x dilution), 25 g / L high-efficiency cyhalothrin emulsifiable concentrate (2000x dilution), 0.004% acetylene-hydroxyadenine soluble powder (750x dilution), enzymatically hydrolyzed seaweed extract microbial inoculant (250x dilution), 8000 IU / µL Bacillus thuringiensis suspension (200x dilution), and nano-network structure control agent (2000x dilution).

[0131] 2. Processing steps (1) Measure 2 L of clean water and pour it into a container. Following the order of pesticide preparation, add 80 g of enzymatically hydrolyzed seaweed microbial agent, 26.67 g of 0.004% acetamiprid·hydroxyadenine soluble powder, 10 g of 25 g / L high-efficiency cyhalothrin EC, 100 g of 8000 IU / µL Bacillus thuringiensis suspending agent, 10 g of red wine, and 10 g of nano-network structure control agent to obtain the mother liquor. During the preparation process, the next agent can only be added after the previous agent has completely dissolved and been stirred evenly.

[0132] (2) Pour the prepared mother liquor into a container, add water to 20 L, stir well, and immerse the roots of the tobacco seedlings in the solution for 15-20 minutes.

[0133] Example 34 1. Formula for tobacco seedling transplanting treatment agent Red wine (2000x dilution), 25 g / L high-efficiency cyhalothrin emulsifiable concentrate (2000x dilution), 0.0016% 28-epi-hypobrachiin lactone aqueous solution (500x dilution), enzymatically hydrolyzed seaweed extract microbial inoculant (250x dilution), 8000 IU / µL Bacillus thuringiensis suspension (200x dilution), and nano-network structure control agent (2000x dilution).

[0134] 2. Processing steps (1) Measure 2 L of clean water and pour it into a container. Following the order of pesticide preparation, add 80 g of enzymatically hydrolyzed seaweed extract microbial agent, 40 g of 0.0016% 28-epibrassinolide aqueous solution, 10 g of 25 g / L high-efficiency cyhalothrin emulsifiable concentrate, 100 g of 8000 IU / µL Bacillus thuringiensis suspension, 10 g of red wine, and 10 g of nano-network structure control agent to obtain the mother liquor. During the preparation process, the next agent can only be added after the previous agent has completely dissolved and been stirred evenly.

[0135] (2) Pour the prepared mother liquor into a container, add water to 20 L, stir well, and immerse the roots of the tobacco seedlings in the solution for 15-20 minutes.

[0136] Example 35 1. Formula for tobacco seedling transplanting treatment agent Red wine (2000x dilution), 25 g / L high-efficiency cyhalothrin emulsifiable concentrate (2000x dilution), 0.004% acetylene·hydroxyacetylene soluble powder (750x dilution), water-soluble fertilizer containing macro-elements (500x dilution), Bacillus thuringiensis suspension (8000 IU / µL) (200x dilution), and nano-network structure control agent (2000x dilution).

[0137] 2. Processing steps (1) Measure 2 L of clean water and pour it into a container. Following the order of pesticide preparation, add 40 g of water-soluble fertilizer containing macro-elements, 26.67 g of 0.004% acetamiprid·hydroxyadenine soluble powder, 10 g of 25 g / L high-efficiency cyhalothrin EC, 100 g of 8000 IU / µL Bacillus thuringiensis SC, 10 g of red wine, and 10 g of nano-network release control agent to obtain the mother liquor. During the preparation process, the next pesticide can only be added after the previous pesticide has completely dissolved and been stirred evenly.

[0138] (2) Pour the prepared mother liquor into a container, add water to 20 L, stir well, and immerse the roots of the tobacco seedlings in the solution for 15-20 minutes.

[0139] Example 36 1. Formula for tobacco seedling transplanting treatment agent Red wine (2000x dilution), 25 g / L high-efficiency cyhalothrin emulsifiable concentrate (2000x dilution), 0.0016% 28-epi-hypobrachiin lactone aqueous solution (500x dilution), macro-element water-soluble fertilizer (500x dilution), 8000 IU / µL Bacillus thuringiensis suspension (200x dilution), nano-network structure control agent (2000x dilution).

[0140] 2. Processing steps (1) Measure 2 L of clean water and pour it into a container. Following the order of pesticide preparation, add 40 g of water-soluble fertilizer containing macro-elements, 40 g of 0.0016% 28-epibrassinolide aqueous solution, 10 g of 25 g / L high-efficiency cyhalothrin EC, 100 g of 8000 IU / µL Bacillus thuringiensis SC, 10 g of red wine, and 10 g of nano-network structure control agent to obtain the mother liquor. During the preparation process, the next pesticide can only be added after the previous pesticide has completely dissolved and been stirred evenly.

[0141] (2) Pour the prepared mother liquor into a container, add water to 20 L, stir well, and immerse the roots of the tobacco seedlings in the solution for 15-20 minutes.

[0142] Example 37 1. Formula for tobacco seedling transplanting treatment agent 2000 times dilution of baijiu (Chinese liquor), 5500 times dilution of 25% chlorantraniliprole·indoxacarb suspension, 750 times dilution of 0.004% acetamiprid·hydroxyacetamiprid soluble powder, 250 times dilution of enzymatically hydrolyzed seaweed extract microbial agent, 300 times dilution of 10 billion CFU / mL Bacillus amyloliquefaciens KN-527 suspension, and 2000 times dilution of nano-network structure control agent.

[0143] 2. Processing steps (1) Measure 2 L of clean water and pour it into a container. Following the order of pesticide preparation, add 80 g of enzymatically hydrolyzed seaweed extract microbial agent, 26.67 g of 0.004% acetamiprid·hydroxyacetamiprid soluble powder, 3.64 g of 25% chlorantraniliprole·indoxacarb suspension, 67.67 g of 10 billion CFU / mL Bacillus amyloliquefaciens KN-527 suspension, 10 g of white wine, and 10 g of nano-mesh release control agent to obtain the mother liquor. During the preparation process, the next agent can only be added after the previous agent has completely dissolved and been stirred evenly.

[0144] (2) Pour the prepared mother liquor into a container, add water to 20 L, stir well, and immerse the roots of the tobacco seedlings in the solution for 15-20 minutes.

[0145] Example 38 1. Formula for tobacco seedling transplanting treatment agent 2000 times dilution of baijiu (Chinese liquor), 5500 times dilution of 25% chlorantraniliprole·indoxacarb suspension, 500 times dilution of 0.0016% 28-epibrassinolide aqueous solution, 250 times dilution of enzymatically hydrolyzed seaweed extract microbial inoculant, 300 times dilution of 10 billion CFU / mL Bacillus amyloliquefaciens KN-527 suspension, and 2000 times dilution of nano-network structure control agent.

[0146] 2. Processing steps (1) Measure 2 L of clean water and pour it into a container. Following the order of pesticide preparation, add 80 g of enzymatically hydrolyzed seaweed extract microbial agent, 40 g of 0.0016% 28-epibrassinolide aqueous solution, 3.64 g of 25% chlorantraniliprole·indoxacarb suspension, 67.67 g of 10 billion CFU / mL Bacillus amyloliquefaciens KN-527 suspension, 10 g of white wine, and 10 g of nano-mesh release control agent to obtain the mother liquor. During the preparation process, the next pesticide can only be added after the previous pesticide has completely dissolved and been stirred evenly.

[0147] (2) Pour the prepared mother liquor into a container, add water to 20 L, stir well, and immerse the roots of the tobacco seedlings in the solution for 15-20 minutes.

[0148] Example 39 1. Formula for tobacco seedling transplanting treatment agent 2000 times dilution of baijiu (Chinese liquor), 5500 times dilution of 25% chlorantraniliprole·indoxacarb suspension, 750 times dilution of 0.004% acetamiprid·hydroxyacetamiprid soluble powder, 500 times dilution of water-soluble fertilizer containing macro-elements, 300 times dilution of 10 billion CFU / mL Bacillus amyloliquefaciens KN-527 suspension, and 2000 times dilution of nano-network structure control agent.

[0149] 2. Processing steps (1) Measure 2 L of clean water and pour it into a container. Following the order of pesticide preparation, add 40 g of water-soluble fertilizer containing macro-elements, 26.67 g of 0.004% acetamiprid·hydroxyacetamiprid soluble powder, 3.64 g of 25% chlorantraniliprole·indoxacarb suspension, 67.67 g of 10 billion CFU / mL Bacillus amyloliquefaciens KN-527 suspension, 10 g of white wine, and 10 g of nano-mesh release control agent to obtain the mother liquor. During the preparation process, the next pesticide can only be added after the previous pesticide has completely dissolved and been stirred evenly.

[0150] (2) Pour the prepared mother liquor into a container, add water to 20 L, stir well, and immerse the roots of the tobacco seedlings in the solution for 15-20 minutes.

[0151] Example 40 1. Formula for tobacco seedling transplanting treatment agent 2000 times dilution of baijiu (Chinese liquor), 5500 times dilution of 25% chlorantraniliprole·indoxacarb suspension, 500 times dilution of 0.0016% 28-epiobrassinolide aqueous solution, 500 times dilution of water-soluble fertilizer containing macro-elements, 300 times dilution of 10 billion CFU / mL Bacillus amyloliquefaciens KN-527 suspension, and 2000 times dilution of nano-network structure control agent.

[0152] 2. Processing steps (1) Measure 2 L of clean water and pour it into a container. Following the order of pesticide preparation, add 40 g of water-soluble fertilizer containing macro-elements, 40 g of 0.0016% 28-epibrinolytic lactone aqueous solution, 3.64 g of 25% chlorantraniliprole·indoxacarb suspension, 67.67 g of 10 billion CFU / mL Bacillus amyloliquefaciens KN-527 suspension, 10 g of white wine, and 10 g of nano-mesh release control agent to obtain the mother liquor. During the preparation process, the next pesticide can only be added after the previous pesticide has completely dissolved and been stirred evenly.

[0153] (2) Pour the prepared mother liquor into a container, add water to 20 L, stir well, and immerse the roots of the tobacco seedlings in the solution for 15-20 minutes.

[0154] Example 41 1. Formula for tobacco seedling transplanting treatment agent 2000 times dilution of baijiu (Chinese liquor), 5500 times dilution of 25% chlorantraniliprole·indoxacarb suspension, 750 times dilution of 0.004% acetamiprid·hydroxyacetamiprid soluble powder, 250 times dilution of enzymatically hydrolyzed seaweed extract microbial agent, 200 times dilution of 8000 IU / µL Bacillus thuringiensis suspension, and 2000 times dilution of nano-network structure control agent.

[0155] 2. Processing steps (1) Measure 2 L of clean water and pour it into a container. Following the order of pesticide preparation, add 80 g of enzymatically hydrolyzed seaweed extract microbial agent, 26.67 g of 0.004% acetamiprid·hydroxyacetamiprid soluble powder, 3.64 g of 25% chlorantraniliprole·indoxacarb suspension, 100 g of 8000 IU / µL Bacillus thuringiensis suspension, 10 g of white wine, and 10 g of nano-mesh release control agent to obtain the mother liquor. During the preparation process, the next pesticide can only be added after the previous pesticide has completely dissolved and been stirred evenly.

[0156] (2) Pour the prepared mother liquor into a container, add water to 20 L, stir well, and immerse the roots of the tobacco seedlings in the solution for 15-20 minutes.

[0157] Example 42 1. Formula for tobacco seedling transplanting treatment agent 2000 times dilution of baijiu (Chinese liquor), 5500 times dilution of 25% chlorantraniliprole·indoxacarb suspension, 500 times dilution of 0.0016% 28-epi-hypobrachiin lactone aqueous solution, 250 times dilution of enzymatically hydrolyzed seaweed extract microbial agent, 200 times dilution of 8000 IU / µL Bacillus thuringiensis suspension, and 2000 times dilution of nano-network structure control agent.

[0158] 2. Processing steps (1) Measure 2 L of clean water and pour it into a container. Following the order of pesticide preparation, add 80 g of enzymatically hydrolyzed seaweed extract microbial agent, 40 g of 0.0016% 28-epibrassinolide aqueous solution, 3.64 g of 25% chlorantraniliprole·indoxacarb suspension, 100 g of 8000 IU / µL Bacillus thuringiensis suspension, 10 g of white wine, and 10 g of nano-mesh release control agent to obtain the mother liquor. During the preparation process, the next pesticide can only be added after the previous pesticide has completely dissolved and been stirred evenly.

[0159] (2) Pour the prepared mother liquor into a container, add water to 20 L, stir well, and immerse the roots of the tobacco seedlings in the solution for 15-20 minutes.

[0160] Example 43 1. Formula for tobacco seedling transplanting treatment agent 2000 times dilution of baijiu (Chinese liquor), 5500 times dilution of 25% chlorantraniliprole·indoxacarb suspension, 750 times dilution of 0.004% acetamiprid·hydroxyacetamiprid soluble powder, 500 times dilution of water-soluble fertilizer containing macro-elements, 2000 times dilution of 8000 IU / µL Bacillus thuringiensis suspension, and 2000 times dilution of nano-network structure control agent.

[0161] 2. Processing steps (1) Measure 2 L of clean water and pour it into a container. Following the order of pesticide preparation, add 40 g of water-soluble fertilizer containing macro-elements, 26.67 g of 0.004% acetamiprid·hydroxyacetamiprid soluble powder, 3.64 g of 25% chlorantraniliprole·indoxacarb suspension, 100 g of 8000 IU / µL Bacillus thuringiensis suspension, 10 g of white wine, and 10 g of nano-mesh release control agent to obtain the mother liquor. During the preparation process, the next pesticide can only be added after the previous pesticide has completely dissolved and been stirred evenly.

[0162] (2) Pour the prepared mother liquor into a container, add water to 20 L, stir well, and immerse the roots of the tobacco seedlings in the solution for 15-20 minutes.

[0163] Example 44 1. Formula for tobacco seedling transplanting treatment agent 2000 times dilution of baijiu (Chinese liquor), 5500 times dilution of 25% chlorantraniliprole·indoxacarb suspension, 500 times dilution of 0.0016% 28-epibrassinolide aqueous solution, 500 times dilution of water-soluble fertilizer containing macro-elements, 200 times dilution of 8000 IU / µL Bacillus thuringiensis suspension, and 2000 times dilution of nano-network structure control agent.

[0164] 2. Processing steps (1) Measure 2 L of clean water and pour it into a container. Following the order of pesticide preparation, add 40 g of water-soluble fertilizer containing macro-elements, 40 g of 0.0016% 28-epibrassinolide aqueous solution, 3.64 g of 25% chlorantraniliprole·indoxacarb suspension, 100 g of 8000 IU / µL Bacillus thuringiensis suspension, 10 g of white wine, and 10 g of nano-mesh release control agent to obtain the mother liquor. During the preparation process, the next pesticide can only be added after the previous pesticide has completely dissolved and been stirred evenly.

[0165] (2) Pour the prepared mother liquor into a container, add water to 20 L, stir well, and immerse the roots of the tobacco seedlings in the solution for 15-20 minutes.

[0166] Example 45 1. Formula for tobacco seedling transplanting treatment agent 2000 times dilution of baijiu (Chinese liquor), 2000 times dilution of 25 g / L high-efficiency cyhalothrin emulsifiable concentrate, 750 times dilution of 0.004% ethanoyl-hydroxyethanoate soluble powder, 250 times dilution of enzymatically hydrolyzed seaweed extract microbial inoculant, 300 times dilution of 10 billion CFU / mL Bacillus amyloliquefaciens KN-527 suspension concentrate, and 2000 times dilution of nano-network structure control agent.

[0167] 2. Processing steps (1) Measure 2 L of clean water and pour it into a container. Following the order of pesticide preparation, add 80 g of enzymatically hydrolyzed seaweed extract microbial agent, 26.67 g of 0.004% acetylene-hydroxyadenine soluble powder, 10 g of 25 g / L high-efficiency cyhalothrin emulsifiable concentrate, 67.67 g of 10 billion CFU / mL Bacillus amyloliquefaciens KN-527 suspension concentrate, 10 g of white wine, and 10 g of nano-network structure control agent to obtain the mother liquor. During the preparation process, the next agent can only be added after the previous agent has completely dissolved and been stirred evenly.

[0168] (2) Pour the prepared mother liquor into a container, add water to 20 L, stir well, and immerse the roots of the tobacco seedlings in the solution for 15-20 minutes.

[0169] Example 46 1. Formula for tobacco seedling transplanting treatment agent 2000 times dilution of baijiu (Chinese liquor), 2000 times dilution of 25 g / L high-efficiency cyhalothrin emulsifiable concentrate, 500 times dilution of 0.0016% 28-epi-hypobrachiin lactone aqueous solution, 250 times dilution of enzymatically hydrolyzed seaweed extract microbial inoculant, 300 times dilution of 10 billion CFU / mL Bacillus amyloliquefaciens KN-527 suspension concentrate, and 2000 times dilution of nano-network structure control agent.

[0170] 2. Processing steps (1) Measure 2 L of clean water and pour it into a container. Following the order of pesticide preparation, add 80 g of enzymatically hydrolyzed seaweed extract microbial agent, 40 g of 0.0016% 28-epibrassinolide aqueous solution, 10 g of 25 g / L high-efficiency cyhalothrin emulsifiable concentrate, 67.67 g of 10 billion CFU / mL Bacillus amyloliquefaciens KN-527 suspension concentrate, 10 g of white wine, and 10 g of nano-network structure control agent to obtain the mother liquor. During the preparation process, the next agent can only be added after the previous agent has completely dissolved and been stirred evenly.

[0171] (2) Pour the prepared mother liquor into a container, add water to 20 L, stir well, and immerse the roots of the tobacco seedlings in the solution for 15-20 minutes.

[0172] Example 47 1. Formula for tobacco seedling transplanting treatment agent 2000 times dilution of baijiu (Chinese liquor), 2000 times dilution of 25 g / L high-efficiency cyhalothrin emulsifiable concentrate, 750 times dilution of 0.004% ethoxylated adenosine monophosphate soluble powder, 500 times dilution of water-soluble fertilizer containing macro-elements, 300 times dilution of 10 billion CFU / mL Bacillus amyloliquefaciens KN-527 suspension concentrate, and 2000 times dilution of nano-network structure control agent.

[0173] 2. Processing steps (1) Measure 2 L of clean water and pour it into a container. Following the order of pesticide preparation, add 40 g of water-soluble fertilizer containing macro-elements, 26.67 g of 0.004% acetamiprid·hydroxyadenine soluble powder, 10 g of 25 g / L high-efficiency cyhalothrin EC, 67.67 g of 10 billion CFU / mL Bacillus amyloliquefaciens KN-527 suspending agent, 10 g of baijiu (Chinese liquor), and 10 g of nano-network release control agent to obtain the mother liquor. During the preparation process, the next pesticide can only be added after the previous pesticide has completely dissolved and been stirred evenly.

[0174] (2) Pour the prepared mother liquor into a container, add water to 20 L, stir well, and immerse the roots of the tobacco seedlings in the solution for 15-20 minutes.

[0175] Example 48 1. Formula for tobacco seedling transplanting treatment agent 2000 times dilution of baijiu (Chinese liquor), 2000 times dilution of 25 g / L high-efficiency cyhalothrin emulsifiable concentrate, 500 times dilution of 0.0016% 28-epi-hypobrachiin lactone aqueous solution, 500 times dilution of water-soluble fertilizer containing macro-elements, 300 times dilution of 10 billion CFU / mL Bacillus amyloliquefaciens KN-527 suspension concentrate, and 2000 times dilution of nano-network structure control agent.

[0176] 2. Processing steps (1) Measure 2 L of clean water and pour it into a container. Add the following pesticides in the order of preparation: 40 g of water-soluble fertilizer containing macro-elements, 40 g of 0.0016% 28-epibrassinolide aqueous solution, 10 g of 25 g / L high-efficiency cyhalothrin EC, 67.67 g of 10 billion CFU / mL Bacillus amyloliquefaciens KN-527 SC, 10 g of baijiu (Chinese liquor), and 10 g of nano-network release control agent to obtain the mother liquor. During the preparation process, the next pesticide can only be added after the previous pesticide has completely dissolved and been stirred evenly.

[0177] (2) Pour the prepared mother liquor into a container, add water to 20 L, stir well, and immerse the roots of the tobacco seedlings in the solution for 15-20 minutes.

[0178] Example 49 1. Formula for tobacco seedling transplanting treatment agent 2000 times dilution of baijiu (Chinese liquor), 2000 times dilution of 25 g / L high-efficiency cyhalothrin emulsifiable concentrate, 750 times dilution of 0.004% ethanoyl-hydroxyethanoate soluble powder, 250 times dilution of enzymatically hydrolyzed seaweed extract microbial inoculant, 2000 times dilution of 8000 IU / µL Bacillus thuringiensis suspension, and 2000 times dilution of nano-network structure control agent.

[0179] 2. Processing steps (1) Measure 2 L of clean water and pour it into a container. Following the order of pesticide preparation, add 80 g of enzymatically hydrolyzed seaweed microbial agent, 26.67 g of 0.004% acetamiprid·hydroxyadenine soluble powder, 10 g of 25 g / L high-efficiency cyhalothrin EC, 100 g of 8000 IU / µL Bacillus thuringiensis suspension, 10 g of baijiu (Chinese liquor), and 10 g of nano-mesh release control agent to obtain the mother liquor. During the preparation process, the next agent can only be added after the previous agent has completely dissolved and been stirred evenly.

[0180] (2) Pour the prepared mother liquor into a container, add water to 20 L, stir well, and immerse the roots of the tobacco seedlings in the solution for 15-20 minutes.

[0181] Example 50 1. Formula for tobacco seedling transplanting treatment agent 2000 times dilution of baijiu (Chinese liquor), 2000 times dilution of 25 g / L high-efficiency cyhalothrin emulsifiable concentrate, 500 times dilution of 0.0016% 28-epi-hypobrachiin lactone aqueous solution, 250 times dilution of enzymatically hydrolyzed seaweed extract microbial inoculant, 2000 times dilution of 8000 IU / µL Bacillus thuringiensis suspension, and 2000 times dilution of nano-network structure control agent.

[0182] 2. Processing steps (1) Measure 2 L of clean water and pour it into a container. Following the order of pesticide preparation, add 80 g of enzymatically hydrolyzed seaweed extract microbial agent, 40 g of 0.0016% 28-epibrassinolide aqueous solution, 10 g of 25 g / L high-efficiency cyhalothrin EC, 100 g of 8000 IU / µL Bacillus thuringiensis SC, 10 g of baijiu (Chinese liquor), and 10 g of nano-network structure control agent to obtain the mother liquor. During the preparation process, the next agent can only be added after the previous agent has completely dissolved and been stirred evenly.

[0183] (2) Pour the prepared mother liquor into a container, add water to 20 L, stir well, and immerse the roots of the tobacco seedlings in the solution for 15-20 minutes.

[0184] Example 51 1. Formula for tobacco seedling transplanting treatment agent 2000 times dilution of baijiu (Chinese liquor), 2000 times dilution of 25 g / L high-efficiency cyhalothrin emulsifiable concentrate, 750 times dilution of 0.004% ethoxylated adenosine monophosphate soluble powder, 500 times dilution of water-soluble fertilizer containing macro-elements, 2000 times dilution of 8000 IU / µL Bacillus thuringiensis suspension, and 2000 times dilution of nano-network structure control agent.

[0185] 2. Processing steps (1) Measure 2 L of clean water and pour it into a container. Following the order of pesticide preparation, add 40 g of water-soluble fertilizer containing macro-elements, 26.67 g of 0.004% acetamiprid·hydroxyadenine soluble powder, 10 g of 25 g / L high-efficiency cyhalothrin EC, 100 g of 8000 IU / µL Bacillus thuringiensis SC, 10 g of baijiu (Chinese liquor), and 10 g of nano-mesh release control agent to obtain the mother liquor. During the preparation process, the next pesticide can only be added after the previous pesticide has completely dissolved and been stirred evenly.

[0186] (2) Pour the prepared mother liquor into a container, add water to 20 L, stir well, and immerse the roots of the tobacco seedlings in the solution for 15-20 minutes.

[0187] Example 52 1. Formula for tobacco seedling transplanting treatment agent 2000 times dilution of baijiu (Chinese liquor), 2000 times dilution of 25 g / L high-efficiency cyhalothrin emulsifiable concentrate, 500 times dilution of 0.0016% 28-epi-hypobrachiin lactone aqueous solution, 500 times dilution of water-soluble fertilizer containing macro-elements, 200 times dilution of 8000 IU / µL Bacillus thuringiensis suspension, and 2000 times dilution of nano-network structure control agent.

[0188] 2. Processing steps (1) Measure 2 L of clean water and pour it into a container. Following the order of pesticide preparation, add 40 g of water-soluble fertilizer containing macro-elements, 40 g of 0.0016% 28-epibrassinolide aqueous solution, 10 g of 25 g / L high-efficiency cyhalothrin EC, 100 g of 8000 IU / µL Bacillus thuringiensis SC, 10 g of baijiu (Chinese liquor), and 10 g of nano-network structure control agent to obtain the mother liquor. During the preparation process, the next pesticide can only be added after the previous pesticide has completely dissolved and been stirred evenly.

[0189] (2) Pour the prepared mother liquor into a container, add water to 20 L, stir well, and immerse the roots of the tobacco seedlings in the solution for 15-20 minutes.

[0190] Example 53 1. Formula for tobacco seedling transplanting treatment agent 2000 times dilution of baijiu (Chinese liquor), 7000 times dilution of 25% chlorantraniliprole·indoxacarb suspension, 875 times dilution of 0.004% acetamiprid·hydroxyacetamiprid soluble powder, 285 times dilution of enzymatically hydrolyzed seaweed extract microbial agent, 375 times dilution of 10 billion CFU / mL Bacillus amyloliquefaciens KN-527 suspension, and 2000 times dilution of nano-network structure control agent.

[0191] 2. Processing steps (1) Measure 2 L of clean water and pour it into a container. Following the order of pesticide preparation, add 70.18 g of enzymatically hydrolyzed seaweed extract microbial agent, 22.86 g of 0.004% acetamiprid·hydroxyacetamiprid soluble powder, 2.86 g of 25% chlorantraniliprole·indoxacarb suspension, 53.33 g of 10 billion CFU / mL Bacillus amyloliquefaciens KN-527 suspension, 10 g of white wine, and 10 g of nano-mesh release control agent to obtain the mother liquor. During the preparation process, the next agent can only be added after the previous agent has completely dissolved and been stirred evenly.

[0192] (2) Pour the prepared mother liquor into a container, add water to 20 L, stir well, and immerse the roots of the tobacco seedlings in the solution for 15-20 minutes.

[0193] Example 54 1. Formula for tobacco seedling transplanting treatment agent 1750 times dilution of baijiu (Chinese liquor), 7000 times dilution of 25% chlorantraniliprole·indoxacarb suspension, 875 times dilution of 0.004% acetamiprid·hydroxyacetamiprid soluble powder, 285 times dilution of enzymatically hydrolyzed seaweed extract microbial inoculant, 375 times dilution of 10 billion CFU / mL Bacillus amyloliquefaciens KN-527 suspension, and 2000 times dilution of nano-network structure control agent.

[0194] 2. Processing steps (1) Measure 2 L of clean water and pour it into a container. Following the order of pesticide preparation, add 70.18 g of enzymatically hydrolyzed seaweed extract microbial agent, 22.86 g of 0.004% acetamiprid·hydroxyacetamiprid soluble powder, 2.86 g of 25% chlorantraniliprole·indoxacarb suspension, 53.33 g of 10 billion CFU / mL Bacillus amyloliquefaciens KN-527 suspension, 11.43 g of white wine, and 10 g of nano-mesh release control agent to obtain the mother liquor. During the preparation process, the next agent can only be added after the previous agent has completely dissolved and been stirred evenly.

[0195] (2) Pour the prepared mother liquor into a container, add water to 20 L, stir well, and immerse the roots of the tobacco seedlings in the solution for 15-20 minutes.

[0196] Example 55 1. Formula for tobacco seedling transplanting treatment agent 1500 times dilution of baijiu (Chinese liquor), 7000 times dilution of 25% chlorantraniliprole·indoxacarb suspension, 875 times dilution of 0.004% acetamiprid·hydroxyacetamiprid soluble powder, 285 times dilution of enzymatically hydrolyzed seaweed extract microbial agent, 375 times dilution of 10 billion CFU / mL Bacillus amyloliquefaciens KN-527 suspension, and 2000 times dilution of nano-network structure control agent.

[0197] 2. Processing steps (1) Measure 2 L of clean water and pour it into a container. Following the order of pesticide preparation, add 70.18 g of enzymatically hydrolyzed seaweed extract microbial agent, 22.86 g of 0.004% acetamiprid·hydroxyacetamiprid soluble powder, 2.86 g of 25% chlorantraniliprole·indoxacarb suspension, 53.33 g of 10 billion CFU / mL Bacillus amyloliquefaciens KN-527 suspension, 13.33 g of white wine, and 10 g of nano-mesh release control agent to obtain the mother liquor. During the preparation process, the next pesticide can only be added after the previous pesticide has completely dissolved and been stirred evenly.

[0198] (2) Pour the prepared mother liquor into a container, add water to 20 L, stir well, and immerse the roots of the tobacco seedlings in the solution for 15-20 minutes.

[0199] Example 56 1. Formula for tobacco seedling transplanting treatment agent 1250 times dilution of baijiu (Chinese liquor), 7000 times dilution of 25% chlorantraniliprole·indoxacarb suspension, 875 times dilution of 0.004% acetamiprid·hydroxyacetamiprid soluble powder, 285 times dilution of enzymatically hydrolyzed seaweed extract microbial agent, 375 times dilution of 10 billion CFU / mL Bacillus amyloliquefaciens KN-527 suspension, and 2000 times dilution of nano-network structure control agent.

[0200] 2. Processing steps (1) Measure 2 L of clean water and pour it into a container. Following the order of pesticide preparation, add 70.18 g of enzymatically hydrolyzed seaweed extract microbial agent, 22.86 g of 0.004% acetamiprid·hydroxyacetamiprid soluble powder, 2.86 g of 25% chlorantraniliprole·indoxacarb suspension, 53.33 g of 10 billion CFU / mL Bacillus amyloliquefaciens KN-527 suspension, 16 g of white wine, and 10 g of nano-mesh release control agent to obtain the mother liquor. During the preparation process, the next agent can only be added after the previous agent has completely dissolved and been stirred evenly.

[0201] (2) Pour the prepared mother liquor into a container, add water to 20 L, stir well, and immerse the roots of the tobacco seedlings in the solution for 15-20 minutes.

[0202] Example 57 1. Formula for tobacco seedling transplanting treatment agent 1750 times dilution of baijiu (Chinese liquor), 5500 times dilution of 25% chlorantraniliprole·indoxacarb suspension, 875 times dilution of 0.004% acetamiprid·hydroxyacetamiprid soluble powder, 285 times dilution of enzymatically hydrolyzed seaweed extract microbial inoculant, 375 times dilution of 10 billion CFU / mL Bacillus amyloliquefaciens KN-527 suspension, and 2000 times dilution of nano-network structure control agent.

[0203] 2. Processing steps (1) Measure 2 L of clean water and pour it into a container. Following the order of pesticide preparation, add 70.18 g of enzymatically hydrolyzed seaweed extract microbial agent, 22.86 g of 0.004% acetamiprid·hydroxyacetamiprid soluble powder, 3.64 g of 25% chlorantraniliprole·indoxacarb suspension, 53.33 g of 10 billion CFU / mL Bacillus amyloliquefaciens KN-527 suspension, 11.43 g of white wine, and 10 g of nano-mesh release control agent to obtain the mother liquor. During the preparation process, the next agent can only be added after the previous agent has completely dissolved and been stirred evenly.

[0204] (2) Pour the prepared mother liquor into a container, add water to 20 L, stir well, and immerse the roots of the tobacco seedlings in the solution for 15-20 minutes.

[0205] Example 58 1. Formula for tobacco seedling transplanting treatment agent 1750 times dilution of baijiu (Chinese liquor), 4000 times dilution of 25% chlorantraniliprole·indoxacarb suspension, 875 times dilution of 0.004% acetamiprid·hydroxyacetamiprid soluble powder, 285 times dilution of enzymatically hydrolyzed seaweed extract microbial agent, 375 times dilution of 10 billion CFU / mL Bacillus amyloliquefaciens KN-527 suspension, and 2000 times dilution of nano-network structure control agent.

[0206] 2. Processing steps (1) Measure 2 L of clean water and pour it into a container. Following the order of pesticide preparation, add 70.18 g of enzymatically hydrolyzed seaweed extract microbial agent, 22.86 g of 0.004% acetamiprid·hydroxyacetamiprid soluble powder, 5 g of 25% chlorantraniliprole·indoxacarb suspension, 53.33 g of 10 billion CFU / mL Bacillus amyloliquefaciens KN-527 suspension, 11.43 g of white wine, and 10 g of nano-mesh release control agent to obtain the mother liquor. During the preparation process, the next agent can only be added after the previous agent has completely dissolved and been stirred evenly.

[0207] (2) Pour the prepared mother liquor into a container, add water to 20 L, stir well, and immerse the roots of the tobacco seedlings in the solution for 15-20 minutes.

[0208] Example 59 1. Formula for tobacco seedling transplanting treatment agent 1750 times dilution of baijiu (Chinese liquor), 2500 times dilution of 25% chlorantraniliprole·indoxacarb suspension, 875 times dilution of 0.004% acetamiprid·hydroxyacetamiprid soluble powder, 285 times dilution of enzymatically hydrolyzed seaweed extract microbial inoculant, 375 times dilution of 10 billion CFU / mL Bacillus amyloliquefaciens KN-527 suspension, and 2000 times dilution of nano-network structure control agent.

[0209] 2. Processing steps (1) Measure 2 L of clean water and pour it into a container. Following the order of pesticide preparation, add 70.18 g of enzymatically hydrolyzed seaweed extract microbial agent, 22.86 g of 0.004% acetamiprid·hydroxyacetamiprid soluble powder, 8 g of 25% chlorantraniliprole·indoxacarb suspension, 53.33 g of 10 billion CFU / mL Bacillus amyloliquefaciens KN-527 suspension, 11.43 g of white wine, and 10 g of nano-mesh release control agent to obtain the mother liquor. During the preparation process, the next pesticide can only be added after the previous pesticide has completely dissolved and been stirred evenly.

[0210] (2) Pour the prepared mother liquor into a container, add water to 20 L, stir well, and immerse the roots of the tobacco seedlings in the solution for 15-20 minutes.

[0211] Example 60 1. Formula for tobacco seedling transplanting treatment agent 1750 times dilution of baijiu (Chinese liquor), 1000 times dilution of 25% chlorantraniliprole·indoxacarb suspension, 875 times dilution of 0.004% acetamiprid·hydroxyacetamiprid soluble powder, 285 times dilution of enzymatically hydrolyzed seaweed extract microbial inoculant, 375 times dilution of 10 billion CFU / mL Bacillus amyloliquefaciens KN-527 suspension, and 2000 times dilution of nano-network structure control agent.

[0212] 2. Processing steps (1) Measure 2 L of clean water and pour it into a container. Following the order of pesticide preparation, add 70.18 g of enzymatically hydrolyzed seaweed extract microbial agent, 22.86 g of 0.004% acetamiprid·hydroxyacetamiprid soluble powder, 20 g of 25% chlorantraniliprole·indoxacarb suspension, 53.33 g of 10 billion CFU / mL Bacillus amyloliquefaciens KN-527 suspension, 11.43 g of white wine, and 10 g of nano-mesh release control agent to obtain the mother liquor. During the preparation process, the next agent can only be added after the previous agent has completely dissolved and been stirred evenly.

[0213] (2) Pour the prepared mother liquor into a container, add water to 20 L, stir well, and immerse the roots of the tobacco seedlings in the solution for 15-20 minutes.

[0214] Example 61 1. Formula for tobacco seedling transplanting treatment agent 1750 times dilution of baijiu (Chinese liquor), 4000 times dilution of 25% chlorantraniliprole·indoxacarb suspension, 750 times dilution of 0.004% acetamiprid·hydroxyacetamiprid soluble powder, 285 times dilution of enzymatically hydrolyzed seaweed extract microbial inoculant, 375 times dilution of 10 billion CFU / mL Bacillus amyloliquefaciens KN-527 suspension, and 2000 times dilution of nano-network structure control agent.

[0215] 2. Processing steps (1) Measure 2 L of clean water and pour it into a container. Following the order of pesticide preparation, add 70.18 g of enzymatically hydrolyzed seaweed extract microbial agent, 26.67 g of 0.004% acetamiprid·hydroxyacetamiprid soluble powder, 5 g of 25% chlorantraniliprole·indoxacarb suspension, 53.33 g of 10 billion CFU / mL Bacillus amyloliquefaciens KN-527 suspension, 11.43 g of white wine, and 10 g of nano-mesh release control agent to obtain the mother liquor. During the preparation process, the next agent can only be added after the previous agent has completely dissolved and been stirred evenly.

[0216] (2) Pour the prepared mother liquor into a container, add water to 20 L, stir well, and immerse the roots of the tobacco seedlings in the solution for 15-20 minutes.

[0217] Example 62 1. Formula for tobacco seedling transplanting treatment agent 1750 times dilution of baijiu (Chinese liquor), 4000 times dilution of 25% chlorantraniliprole·indoxacarb suspension, 625 times dilution of 0.004% acetamiprid·hydroxyacetamiprid soluble powder, 285 times dilution of enzymatically hydrolyzed seaweed extract microbial inoculant, 375 times dilution of 10 billion CFU / mL Bacillus amyloliquefaciens KN-527 suspension, and 2000 times dilution of nano-network structure control agent.

[0218] 2. Processing steps (1) Measure 2 L of clean water and pour it into a container. Following the order of pesticide preparation, add 70.18 g of enzymatically hydrolyzed seaweed extract microbial agent, 32 g of 0.004% acetamiprid·hydroxyacetamiprid soluble powder, 5 g of 25% chlorantraniliprole·indoxacarb suspension, 53.33 g of 10 billion CFU / mL Bacillus amyloliquefaciens KN-527 suspension, 11.43 g of white wine, and 10 g of nano-mesh release control agent to obtain the mother liquor. During the preparation process, the next agent can only be added after the previous agent has completely dissolved and been stirred evenly.

[0219] (2) Pour the prepared mother liquor into a container, add water to 20 L, stir well, and immerse the roots of the tobacco seedlings in the solution for 15-20 minutes.

[0220] Example 63 1. Formula for tobacco seedling transplanting treatment agent 1750 times dilution of baijiu (Chinese liquor), 4000 times dilution of 25% chlorantraniliprole·indoxacarb suspension, 500 times dilution of 0.004% acetamiprid·hydroxyacetamiprid soluble powder, 285 times dilution of enzymatically hydrolyzed seaweed extract microbial agent, 375 times dilution of 10 billion CFU / mL Bacillus amyloliquefaciens KN-527 suspension, and 2000 times dilution of nano-network structure control agent.

[0221] 2. Processing steps (1) Measure 2 L of clean water and pour it into a container. Following the order of pesticide preparation, add 70.18 g of enzymatically hydrolyzed seaweed extract microbial agent, 40 g of 0.004% acetamiprid·hydroxyacetamiprid soluble powder, 5 g of 25% chlorantraniliprole·indoxacarb suspension, 53.33 g of 10 billion CFU / mL Bacillus amyloliquefaciens KN-527 suspension, 11.43 g of white wine, and 10 g of nano-mesh release control agent to obtain the mother liquor. During the preparation process, the next agent can only be added after the previous agent has completely dissolved and been stirred evenly.

[0222] (2) Pour the prepared mother liquor into a container, add water to 20 L, stir well, and immerse the roots of the tobacco seedlings in the solution for 15-20 minutes.

[0223] Example 64 1. Formula for tobacco seedling transplanting treatment agent 1750 times dilution of baijiu (Chinese liquor), 4000 times dilution of 25% chlorantraniliprole·indoxacarb suspension, 375 times dilution of 0.004% acetamiprid·hydroxyacetamiprid soluble powder, 285 times dilution of enzymatically hydrolyzed seaweed extract microbial inoculant, 375 times dilution of 10 billion CFU / mL Bacillus amyloliquefaciens KN-527 suspension, and 2000 times dilution of nano-network structure control agent.

[0224] 2. Processing steps (1) Measure 2 L of clean water and pour it into a container. Following the order of pesticide preparation, add 70.18 g of enzymatically hydrolyzed seaweed microbial agent, 53.33 g of 0.004% acetamiprid·hydroxyacetamiprid soluble powder, 5 g of 25% chlorantraniliprole·indoxacarb suspension, 53.33 g of 10 billion CFU / mL Bacillus amyloliquefaciens KN-527 suspension, 11.43 g of white wine, and 10 g of nano-mesh release control agent to obtain the mother liquor. During the preparation process, the next agent can only be added after the previous agent has completely dissolved and been stirred evenly.

[0225] (2) Pour the prepared mother liquor into a container, add water to 20 L, stir well, and immerse the roots of the tobacco seedlings in the solution for 15-20 minutes.

[0226] Example 65 1. Formula for tobacco seedling transplanting treatment agent 1750 times dilution of baijiu (Chinese liquor), 4000 times dilution of 25% chlorantraniliprole·indoxacarb suspension, 625 times dilution of 0.004% acetamiprid·hydroxyacetamiprid soluble powder, 250 times dilution of enzymatically hydrolyzed seaweed extract microbial inoculant, 375 times dilution of 10 billion CFU / mL Bacillus amyloliquefaciens KN-527 suspension, and 2000 times dilution of nano-network structure control agent.

[0227] 2. Processing steps (1) Measure 2 L of clean water and pour it into a container. Following the order of pesticide preparation, add 80 g of enzymatically hydrolyzed seaweed extract microbial agent, 32 g of 0.004% acetamiprid·hydroxyacetamiprid soluble powder, 5 g of 25% chlorantraniliprole·indoxacarb suspension, 53.33 g of 10 billion CFU / mL Bacillus amyloliquefaciens KN-527 suspension, 11.43 g of white wine, and 10 g of nano-mesh release control agent to obtain the mother liquor. During the preparation process, the next pesticide can only be added after the previous pesticide has completely dissolved and been stirred evenly.

[0228] (2) Pour the prepared mother liquor into a container, add water to 20 L, stir well, and immerse the roots of the tobacco seedlings in the solution for 15-20 minutes.

[0229] Example 66 1. Formula for tobacco seedling transplanting treatment agent 1750 times dilution of baijiu (Chinese liquor), 4000 times dilution of 25% chlorantraniliprole·indoxacarb suspension, 625 times dilution of 0.004% acetamiprid·hydroxyacetamiprid soluble powder, 215 times dilution of enzymatically hydrolyzed seaweed extract microbial inoculant, 375 times dilution of 10 billion CFU / mL Bacillus amyloliquefaciens KN-527 suspension, and 2000 times dilution of nano-network structure control agent.

[0230] 2. Processing steps (1) Measure 2 L of clean water and pour it into a container. Following the order of pesticide preparation, add 93.02 g of enzymatically hydrolyzed seaweed extract microbial agent, 32 g of 0.004% acetamiprid·hydroxyacetamiprid soluble powder, 5 g of 25% chlorantraniliprole·indoxacarb suspension, 53.33 g of 10 billion CFU / mL Bacillus amyloliquefaciens KN-527 suspension, 11.43 g of white wine, and 10 g of nano-mesh release control agent to obtain the mother liquor. During the preparation process, the next agent can only be added after the previous agent has completely dissolved and been stirred evenly.

[0231] (2) Pour the prepared mother liquor into a container, add water to 20 L, stir well, and immerse the roots of the tobacco seedlings in the solution for 15-20 minutes.

[0232] Example 67 1. Formula for tobacco seedling transplanting treatment agent 1750 times dilution of baijiu (Chinese liquor), 4000 times dilution of 25% chlorantraniliprole·indoxacarb suspension, 625 times dilution of 0.004% acetamiprid·hydroxyacetamiprid soluble powder, 180 times dilution of enzymatically hydrolyzed seaweed extract microbial inoculant, 375 times dilution of 10 billion CFU / mL Bacillus amyloliquefaciens KN-527 suspension, and 2000 times dilution of nano-network structure control agent.

[0233] 2. Processing steps (1) Measure 2 L of clean water and pour it into a container. Following the order of pesticide preparation, add 111.11 g of enzymatically hydrolyzed seaweed extract microbial agent, 32 g of 0.004% acetamiprid·hydroxyacetamiprid soluble powder, 5 g of 25% chlorantraniliprole·indoxacarb suspension, 53.33 g of 10 billion CFU / mL Bacillus amyloliquefaciens KN-527 suspension, 11.43 g of white wine, and 10 g of nano-mesh release control agent to obtain the mother liquor. During the preparation process, the next agent can only be added after the previous agent has completely dissolved and been stirred evenly.

[0234] (2) Pour the prepared mother liquor into a container, add water to 20 L, stir well, and immerse the roots of the tobacco seedlings in the solution for 15-20 minutes.

[0235] Example 68 1. Formula for tobacco seedling transplanting treatment agent 1750 times dilution of baijiu (Chinese liquor), 4000 times dilution of 25% chlorantraniliprole·indoxacarb suspension, 625 times dilution of 0.004% acetamiprid·hydroxyacetamiprid soluble powder, 145 times dilution of enzymatically hydrolyzed seaweed extract microbial inoculant, 375 times dilution of 10 billion CFU / mL Bacillus amyloliquefaciens KN-527 suspension, and 2000 times dilution of nano-network structure control agent.

[0236] 2. Processing steps (1) Measure 2 L of clean water and pour it into a container. Following the order of pesticide preparation, add 137.93 g of enzymatically hydrolyzed seaweed extract microbial agent, 32 g of 0.004% acetamiprid·hydroxyacetamiprid soluble powder, 5 g of 25% chlorantraniliprole·indoxacarb suspension, 53.33 g of 10 billion CFU / mL Bacillus amyloliquefaciens KN-527 suspension, 11.43 g of white wine, and 10 g of nano-mesh release control agent to obtain the mother liquor. During the preparation process, the next agent can only be added after the previous agent has completely dissolved and been stirred evenly.

[0237] (2) Pour the prepared mother liquor into a container, add water to 20 L, stir well, and immerse the roots of the tobacco seedlings in the solution for 15-20 minutes.

[0238] Example 69 1. Formula for tobacco seedling transplanting treatment agent 1750 times dilution of baijiu (Chinese liquor), 4000 times dilution of 25% chlorantraniliprole·indoxacarb suspension, 625 times dilution of 0.004% acetamiprid·hydroxyacetamiprid soluble powder, 215 times dilution of enzymatically hydrolyzed seaweed extract microbial inoculant, 300 times dilution of 10 billion CFU / mL Bacillus amyloliquefaciens KN-527 suspension, and 2000 times dilution of nano-network structure control agent.

[0239] 2. Processing steps (1) Measure 2 L of clean water and pour it into a container. Following the order of pesticide preparation, add 93.02 g of enzymatically hydrolyzed seaweed extract microbial agent, 32 g of 0.004% acetamiprid·hydroxyacetamiprid soluble powder, 5 g of 25% chlorantraniliprole·indoxacarb suspension, 67.67 g of 10 billion CFU / mL Bacillus amyloliquefaciens KN-527 suspension, 11.43 g of white wine, and 10 g of nano-mesh release control agent to obtain the mother liquor. During the preparation process, the next agent can only be added after the previous agent has completely dissolved and been stirred evenly.

[0240] (2) Pour the prepared mother liquor into a container, add water to 20 L, stir well, and immerse the roots of the tobacco seedlings in the solution for 15-20 minutes.

[0241] Example 70 1. Formula for tobacco seedling transplanting treatment agent 1750 times dilution of baijiu (Chinese liquor), 4000 times dilution of 25% chlorantraniliprole·indoxacarb suspension, 625 times dilution of 0.004% acetamiprid·hydroxyacetamiprid soluble powder, 215 times dilution of enzymatically hydrolyzed seaweed extract microbial inoculant, 225 times dilution of 10 billion CFU / mL Bacillus amyloliquefaciens KN-527 suspension, and 2000 times dilution of nano-network structure control agent.

[0242] 2. Processing steps (1) Measure 2 L of clean water and pour it into a container. Following the order of pesticide preparation, add 93.02 g of enzymatically hydrolyzed seaweed extract microbial agent, 32 g of 0.004% acetamiprid·hydroxyacetamiprid soluble powder, 5 g of 25% chlorantraniliprole·indoxacarb suspension, 88.89 g of 10 billion CFU / mL Bacillus amyloliquefaciens KN-527 suspension, 11.43 g of white wine, and 10 g of nano-mesh release control agent to obtain the mother liquor. During the preparation process, the next agent can only be added after the previous agent has completely dissolved and been stirred evenly.

[0243] (2) Pour the prepared mother liquor into a container, add water to 20 L, stir well, and immerse the roots of the tobacco seedlings in the solution for 15-20 minutes.

[0244] Example 71 1. Formula for tobacco seedling transplanting treatment agent 1750 times dilution of baijiu (Chinese liquor), 4000 times dilution of 25% chlorantraniliprole·indoxacarb suspension, 625 times dilution of 0.004% acetamiprid·hydroxyacetamiprid soluble powder, 215 times dilution of enzymatically hydrolyzed seaweed extract microbial inoculant, 150 times dilution of 10 billion CFU / mL Bacillus amyloliquefaciens KN-527 suspension, and 2000 times dilution of nano-network structure control agent.

[0245] 2. Processing steps (1) Measure 2 L of clean water and pour it into a container. Following the order of pesticide preparation, add 93.02 g of enzymatically hydrolyzed seaweed extract microbial agent, 32 g of 0.004% acetamiprid·hydroxyacetamiprid soluble powder, 5 g of 25% chlorantraniliprole·indoxacarb suspension, 133.33 g of 10 billion CFU / mL Bacillus amyloliquefaciens KN-527 suspension, 11.43 g of white wine, and 10 g of nano-mesh release control agent to obtain the mother liquor. During the preparation process, the next agent can only be added after the previous agent has completely dissolved and been stirred evenly.

[0246] (2) Pour the prepared mother liquor into a container, add water to 20 L, stir well, and immerse the roots of the tobacco seedlings in the solution for 15-20 minutes.

[0247] Example 72 1. Formula for tobacco seedling transplanting treatment agent 1750 times dilution of baijiu (Chinese liquor), 4000 times dilution of 25% chlorantraniliprole·indoxacarb suspension, 625 times dilution of 0.004% acetamiprid·hydroxyacetamiprid soluble powder, 215 times dilution of enzymatically hydrolyzed seaweed extract microbial inoculant, 75 times dilution of 10 billion CFU / mL Bacillus amyloliquefaciens KN-527 suspension, and 2000 times dilution of nano-network structure control agent.

[0248] 2. Processing steps (1) Measure 2 L of clean water and pour it into a container. Following the order of pesticide preparation, add 93.02 g of enzymatically hydrolyzed seaweed extract microbial agent, 32 g of 0.004% acetamiprid·hydroxyacetamiprid soluble powder, 5 g of 25% chlorantraniliprole·indoxacarb suspension, 267.67 g of 10 billion CFU / mL Bacillus amyloliquefaciens KN-527 suspension, 11.43 g of white wine, and 10 g of nano-mesh release control agent to obtain the mother liquor. During the preparation process, the next pesticide can only be added after the previous pesticide has completely dissolved and been stirred evenly.

[0249] (2) Pour the prepared mother liquor into a container, add water to 20 L, stir well, and immerse the roots of the tobacco seedlings in the solution for 15-20 minutes.

[0250] Experimental Example 1 Seven days before the tobacco seedlings emerged from the seedling shed, the leaves of the tobacco seedlings were sprayed with the solution described in Examples 1-21, at a dosage of 50 mL per seedling tray. The seedling trays were 168-cell trays.

[0251] On the day of transplanting, the roots of the tobacco seedlings were soaked using the methods described in Examples 22-71, with the soaking time controlled between 15-20 minutes. The specific time was adjusted flexibly according to the number of seedlings in the container: the soaking time was appropriately extended when there were more seedlings and shortened accordingly when there were fewer seedlings. Specifically, the soaking time was 15 minutes when there were 168 seedlings and 20 minutes when there were 252 seedlings.

[0252] During soaking, the liquid level should not exceed 1 cm above the junction of the tobacco seedling roots and stems. After each soaking, the treatment agent should be replaced promptly to ensure consistent treatment results.

[0253] When transplanting tobacco seedlings from the nursery, operators must not smoke and should avoid touching the seedlings with their hands. When transporting the seedlings to the transplanting site, place them in a cool, hygienic environment. Do not transplant the seedlings after the roots have been dripping wet.

[0254] The condition of the tobacco seedlings in Examples 1-20 was investigated, and the specific procedures were as follows: During the investigation, five trays of tobacco seedlings were randomly selected from each treatment to observe the overall condition of the seedlings, including leaf color, leaf deformity, leaf pesticide damage, uniformity of the plant population, root condition, and stem flexibility. Then, 20 seedlings were randomly selected from each tray, for a total of 100 seedlings. The number of seedlings with deformities and pesticide damage, viral diseases, anthracnose, damping-off, and seedling blight was investigated. The rates of deformity and pesticide damage, viral disease incidence, anthracnose incidence, damping-off incidence, and seedling blight incidence were calculated. The number of robust seedlings was counted, calculated by subtracting the number of seedlings with deformities and pesticide damage, viral diseases, anthracnose, damping-off, and seedling blight from the total number of seedlings investigated. The criteria for robust seedlings were: seedlings fully enclosed in the tray, green leaves, 5-6 true leaves; stem height 4-6 cm, good flexibility; well-developed root system; no pests or diseases; and uniform plant population. The robust seedling rate was then calculated.

[0255] The optimal treatment effect for tobacco seedlings is: a seedling survival rate of over 85% per tray, of which the rate of robust seedlings is over 95%.

[0256] Experimental Example 2 The condition of the tobacco seedlings in Examples 21-72 was investigated, and the specific procedures were as follows: The first survey was conducted 14 days after transplanting. A 5-point sampling method was used for each replicate, with 20 seedlings randomly selected from each point. Each treatment was replicated 5 times, for a total of 500 seedlings surveyed. The number of seedlings that died naturally and the number of seedlings damaged by underground pests were counted, and the survival rate and the rate of damage were calculated. The second survey was conducted 21 days after transplanting. A 5-point sampling method was used for each replicate, with 20 seedlings randomly selected from each point. Each treatment was replicated 5 times, for a total of 500 seedlings surveyed. The number of seedlings infected with root black rot, viral diseases, and black shank diseases was investigated, and the incidence rates were calculated.

[0257] The experimental results of Experiments 1 and 2 are shown in Tables 1 and 2.

[0258]

[0259] Table 1 Table 2

[0260] As shown in Table 1, spraying the tobacco seedlings with the treatment agent of Example 1 7 days before the seedlings emerge from the seed shed yielded the best results. The seedlings exhibited the best uniformity, root system, and flexibility, while the incidence of viral diseases, anthracnose, damping-off, and seedling blight was the lowest, and the seedling vigor rate was the highest.

[0261] As shown in Table 2, spraying the tobacco seedlings with the treatment agent of Example 15 7 days before the seedlings emerge from the seed shed was the best in terms of both dosage and final effect. The seedlings had green leaves, no leaf deformities, no pesticide damage, uniform and neat plant population, well-developed root system, flexible branches and trunks, and a strong seedling rate of 99%.

[0262] In Examples 1-20, the same treatment agent components were enadenosyl-hydroxyenadenosyl- ...

[0263] Enyl and hydroxyenyl adenosine stimulate the formation and differentiation of tobacco seedling roots, promote root growth, encourage the formation of lateral roots, and facilitate the establishment and development of the root system. It also regulates the stress resistance of tobacco seedlings, improving their drought and cold resistance. Components containing enyl and hydroxyenyl adenosine result in better seedling condition compared to those containing other components.

[0264] Bacillus amyloliquefaciens KN-527 can improve the soil microecological environment, further enhance the stress resistance of tobacco plants, and has a preventive effect on seedling diseases. Components containing Bacillus amyloliquefaciens KN-527 have lower incidence rates of viral diseases, anthracnose, damping-off, and seedling blight in tobacco seedlings compared to other components.

[0265] According to relevant data, the seedling vigor rate of tobacco seedlings under low-concentration solutions is lower than that of 0.004% adenine·hydroxyadenine soluble powder at a dilution of 625 times + 10 billion CFU / mL Bacillus amyloliquefaciens KN-527 suspension at a dilution of 225 times. Furthermore, 0.004% adenine·hydroxyadenine soluble powder at a dilution of 375 times and Bacillus amyloliquefaciens KN-527 suspension at a dilution of 75 times can cause phytotoxicity and seedling deformities. A dilution of 0.004% adenine·hydroxyadenine soluble powder at a dilution of 500 times and Bacillus amyloliquefaciens KN-527 suspension at a dilution of 15 times... The seedling vigor rate of the 0-fold dilution component is comparable to that of 0.004% acetylene-hydroxyenin soluble powder at 625 times dilution and 10 billion CFU / mL Bacillus amyloliquefaciens KN-527 suspension at 225 times dilution, but its dosage is higher than that of 0.004% acetylene-hydroxyenin soluble powder at 625 times dilution and 10 billion CFU / mL Bacillus amyloliquefaciens KN-527 suspension at 225 times dilution. Therefore, under the same effect, the optimal choice is 0.004% acetylene-hydroxyenin soluble powder at 625 times dilution + 10 billion CFU / mL Bacillus amyloliquefaciens KN-527 suspension at 225 times dilution.

[0266] Table 3

[0267] As shown in Table 3, the best treatment effect was achieved by soaking the roots of the tobacco seedlings in the treatment agent of Example 37 before transplanting. The survival rate of the tobacco seedlings was the highest 14 days after transplanting, and the incidence of root black rot, viral disease and black shank disease was the lowest 21 days after transplanting.

[0268] In Examples 21-52, through further exploration of the optimized combination of alcohol, insecticide, regulator and fungicide, it was found that the best transplanting combination was baijiu + chlorantraniliprole·indoxacarb + acetamiprid·hydroxyacetamiprid + enzymatic hydrolysis of seaweed extract microbial agent + Bacillus amyloliquefaciens KN-527 + nano-network structure.

[0269] As a distilled spirit, baijiu's core component is ethanol, and it has a high concentration. During transplanting, low-dose diluted baijiu primarily relies on the penetrating and disinfecting properties of ethanol to broadly and temporarily inhibit the activity of some harmful microorganisms in the soil surrounding the tobacco seedling roots. Red wine, being a fermented beverage, has a much more complex composition. Besides a small amount of alcohol, it is rich in organic acids (such as tartaric acid and malic acid), polyphenols (such as tannins), residual sugars, amino acids, and trace minerals. These components have more diverse functions when used as transplanting fertilizer additives. However, unexpectedly, this application, by setting different components (baijiu and red wine) in Examples 21 and 37 (with the same components being chlorantraniliprole·indoxacarb, acetamiprid·hydroxyacetamiprid, enzymatically hydrolyzed seaweed extract microbial agent, Bacillus amyloliquefaciens KN-527, and nano-network structure control agent), found that the component containing baijiu resulted in a higher survival rate for tobacco seedlings, and baijiu had a better insecticidal and disinfecting effect on the tobacco seedling roots and transplanting soil.

[0270] Furthermore, in Examples 37 and 45, 25% chlorantraniliprole·indoxacarb suspension and 25 g / L high-efficiency cyhalothrin emulsifiable concentrate were set as different components (the same components were liquor, acetamiprid·hydroxyacetamiprid, enzymatically hydrolyzed seaweed extract microbial agent, Bacillus amyloliquefaciens KN-527, and nano-network control agent). The experimental results showed that the presence of chlorantraniliprole·indoxacarb in this combination resulted in a lower damage rate of underground pests, and chlorantraniliprole·indoxacarb had a better control effect on underground pests.

[0271] In Examples 37 and 38, terpinene-hydroxyadenine and 0.0016% 28-epiglosporin lactone aqueous solution were set as different components (the same components were liquor, chlorantraniliprole-indoxacarb, enzymatic hydrolysis of seaweed extract microbial agent, Bacillus amyloliquefaciens KN-527, and nano-network structure control agent). The experimental results showed that the components containing terpinene-hydroxyadenine in this combination had a higher survival rate of tobacco seedlings 14 days after transplanting.

[0272] In Examples 37 and 39, the enzymatic hydrolysis seaweed extract microbial agent and the water-soluble fertilizer of macroelements were set as different components (the same components were liquor, chlorantraniliprole·indoxacarb, acetamiprid·hydroxyacetamiprid, Bacillus amyloliquefaciens KN-527, and nano-network control agent). The experimental results showed that the components of the enzymatic hydrolysis seaweed extract microbial agent in this combination had a higher survival rate of tobacco seedlings 14 days after transplanting, and a lower incidence of root black rot, viral disease, and black shank disease 21 days after transplanting.

[0273] In Examples 37 and 41, Bacillus amyloliquefaciens KN-527 and 0.0016% 28-epiglosporin lactone aqueous solution were set as different components (the same components were liquor, chlorantraniliprole·indoxacarb, acetamiprid·hydroxyacetamiprid, enzymatic hydrolysis of seaweed extract microbial agent, and nano-network structure control agent). The experimental results showed that the components containing Bacillus amyloliquefaciens KN-527 in this combination resulted in a lower incidence of root black rot, viral disease, and black shank disease 21 days after transplanting of tobacco seedlings.

[0274] Furthermore, in Examples 53-71, the optimal concentrations of baijiu (Chinese liquor), chlorantraniliprole·indoxacarb, acetamiprid·hydroxyadenine, enzymatically hydrolyzed seaweed extract microbial agent, and Bacillus amyloliquefaciens KN-527 were verified one by one. The experimental results showed that Example 70 (1750x baijiu + 4000x 25% chlorantraniliprole·indoxacarb suspension + 625x 0.004% acetamiprid·hydroxyadenine soluble powder + 215x enzymatically hydrolyzed seaweed extract microbial agent + 225x 10 billion CFU / mL Bacillus amyloliquefaciens KN-527 suspension) was the most effective. The experimental results indicate that the concentrations in Example 70 ensured the best results while requiring the least amount of product.

[0275] It should be noted that the specific embodiments described above are exemplary, and those skilled in the art can devise various solutions inspired by the disclosure of this invention, all of which fall within the scope of this invention and its protection. Those skilled in the art should understand that this specification is illustrative and not intended to limit the scope of the claims. The scope of protection of this invention is defined by the claims and their equivalents.

Claims

1. A highly efficient growth treatment agent for tobacco seedling transplantation, characterized in that, It includes Group A for treating tobacco seedling leaves and Group B for treating tobacco seedling roots, wherein... Group A contains hydroxyadenine soluble powder and Bacillus amyloliquefaciens KN-527; Group B contains baijiu (Chinese liquor) with an alcohol content of 45 degrees or higher, chlorantraniliprole·indoxacarb suspension, acetamiprid·hydroxyacetamiprid soluble powder, enzymatically hydrolyzed seaweed extract microorganisms, and Bacillus amyloliquefaciens.

2. The high-efficiency growth treatment agent for tobacco seedling transplanting according to claim 1, characterized in that, Each liter of the high-efficiency growth treatment agent for tobacco seedling transplantation, group A, contains, by weight, 0.67-1.33 parts of 1500-750 times diluted hydroxylamine and 1.67-6.67 parts of 600-150 times diluted Bacillus amyloliquefaciens KN-527.

3. The high-efficiency growth treatment agent for tobacco seedling transplanting according to claim 1, characterized in that, Each liter of the high-efficiency growth treatment agent for tobacco seedling transplantation, group B, contains, by weight, 0.5-0.8 parts of 2000-1250 times diluted liquor, 0.18-1 part of 5500-1000 times diluted chlorantraniliprole·indoxacarb, 1.33-2.67 parts of 750-375 times diluted ethanoate·hydroxyethanoate, 4-6.9 parts of 250-145 times diluted enzymatic hydrolysate seaweed extract microbial agent, and 3.33-13.33 parts of 300-75 times diluted Bacillus amyloliquefaciens KN-527.

4. A method for transplanting tobacco seedlings, characterized in that, It includes the following steps: Before the tobacco seedlings are transplanted, the leaves are sprayed with Group A of high-efficiency growth treatment agent for tobacco seedling transplanting, which contains soluble powder of hydroxyadenine and Bacillus amyloliquefaciens KN-527. When the tobacco seedlings are transplanted, their roots are soaked in Group B of a high-efficiency growth treatment agent for tobacco seedling transplantation, which contains 45-degree or higher liquor, chlorantraniliprole·indoxacarb suspension, acetamiprid·hydroxyacetamiprid soluble powder, enzymatically hydrolyzed seaweed extract microorganisms and Bacillus amyloliquefaciens.

5. The method for transplanting tobacco seedlings according to claim 4, characterized in that, Each liter of the high-efficiency growth treatment agent for tobacco seedling transplantation, group A, contains, by weight, 0.67-1.33 parts of 1500-750 times diluted hydroxylamine and 1.67-6.67 parts of 600-150 times diluted Bacillus amyloliquefaciens KN-527.

6. The method for transplanting tobacco seedlings according to claim 4, characterized in that, Each liter of the high-efficiency growth treatment agent for tobacco seedling transplantation, group B, contains, by weight, 0.5-0.8 parts of 2000-1250 times diluted liquor, 0.18-1 part of 5500-1000 times diluted chlorantraniliprole·indoxacarb, 1.33-2.67 parts of 750-375 times diluted ethanoate·hydroxyethanoate, 4-6.9 parts of 250-145 times diluted enzymatic hydrolysate seaweed extract microbial agent, and 3.33-13.33 parts of 300-75 times diluted Bacillus amyloliquefaciens KN-527.

7. The method for transplanting tobacco seedlings according to claim 4, characterized in that, The spraying time for Group A of the high-efficiency growth treatment agent for tobacco seedling transplanting should be at least 7 days before the seedlings are removed from the greenhouse.

8. The method for transplanting tobacco seedlings according to claim 4, characterized in that, The soaking time for Group B of the high-efficiency growth treatment agent for tobacco seedling transplanting should not exceed 1 day before or 1 day after transplanting.

9. The method for transplanting tobacco seedlings according to claim 4, characterized in that, The soaking time for Group B of the high-efficiency growth treatment agent for tobacco seedling transplanting is 15-20 minutes.

10. The method for transplanting tobacco seedlings according to claim 4, characterized in that, The spraying amount of Group A of the high-efficiency growth treatment agent for tobacco seedling transplanting is 50 mL / seedling tray.

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