Plasma matrix bone block as well as preparation method and application thereof

Abstract

The invention relates to the technical field of medical materials, in particular to a plasma matrix bone block and a preparation method and application thereof. According to the preparation method provided by the invention, the cell-deficient plasma matrix and the bone repair material are mixed to prepare the plasma matrix bone block, a three-dimensional fibrous protein network structure is densified by utilizing a surface heating mode, the degradation performance and the cell barrier function of the plasma matrix bone block are effectively improved, and meanwhile, by injecting a cell concentrated solution enriched with effective biological components, the effective biological components can be effectively degraded. The finally prepared plasma matrix bone block has excellent biological activity. When the prepared plasma matrix bone block is applied to repairing treatment of alveolar burst, soft tissue cells can be prevented from invading a bone regeneration area, the stability of a regeneration space is maintained, and absorption of a transplanted material is delayed. Compared with autogenous bone transplantation, the method has the advantages that a second operation area can be prevented from being developed, meanwhile, the problems of insufficient sources and the like do not need to be considered, and the defects of autogenous bone transplantation are avoided.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of medical materials, in particular to a plasma matrix bone block and a preparation method and application thereof. BACKGROUND

[0002] Alveolar cleft is a kind of congenital maxillofacial deformity, which is characterized by the continuity of the alveolar bone (the front part of the maxilla) being interrupted, often associated with cleft lip and cleft palate. Its occurrence is related to the failure of tissue fusion in the maxillofacial region during embryonic development, which may affect tooth eruption, occlusion function and facial appearance.

[0003] At present, the repair treatment of alveolar cleft mainly adopts surgical method, and autologous bone transplantation is mainly used in clinical treatment, but autologous bone transplantation has problems such as easy absorption, limited amount, long operation time, etc. Especially for children with cleft lip and palate, autologous bone transplantation brings great mental and physical pain to children and their families, and increases the burden of the whole family.

[0004] The plasma matrix technology can improve the properties of bone repair materials, so that they have certain mechanical strength. However, the existing plasma matrix bone block has the problems of fast degradation speed and insufficient self-barrier function. Therefore, it is hoped to provide a new plasma matrix bone block and a preparation method thereof to replace autologous bone transplantation surgery in the scene of alveolar cleft of cleft lip and palate. SUMMARY

[0005] In order to solve the above technical problems, the present application provides a plasma matrix bone block and a preparation method and application thereof. The preparation method provided by the present application mixes the cell-free plasma matrix and the bone repair material to prepare the plasma matrix bone block, and uses surface heating to densify the three-dimensional fibrin network structure, effectively improving the degradation performance and cell barrier function of the plasma matrix bone block. At the same time, by injecting the cell concentrate solution rich in effective biological components, the finally prepared plasma matrix bone block has excellent biological activity. When the plasma matrix bone block prepared by the present application is applied to the repair treatment of alveolar cleft, it can avoid the invasion of soft tissue cells into the bone regeneration area, maintain the stability of the regeneration space, and delay the absorption of the implanted material. Compared with autologous bone transplantation, it can avoid opening a second operation area, and at the same time, it does not need to consider the problem of insufficient source, avoiding the disadvantages of autologous bone transplantation.

[0006] In a first aspect, the present application provides a preparation method of a plasma matrix bone block, which comprises: (1) collecting whole blood to obtain a mixed solution of liquid plasma matrix and intermediate layer of white blood cell-rich layer by first centrifugation, and second centrifugation of the mixed solution to obtain upper cell-free plasma matrix and lower cell concentrate solution; (2) placing the hydrophilic treated bone repair material and the acellular plasma matrix into a hydrophilic treated solid plasma matrix preparation tube and mixing to obtain a plasma matrix bone block gel, shaping to obtain a first intermediate, and heating any surface of the first intermediate to obtain a second intermediate; (3) injecting the cell concentrate into the second intermediate to obtain the plasma matrix bone block.

[0007] The preparation method provided by the application mixes the acellular plasma matrix and the bone repair material to prepare the plasma matrix bone block, and densifies the three-dimensional fibrin network structure by surface heating, wherein the fibrin scaffold in the plasma matrix can stabilize the bone repair material, provide certain mechanical strength to the plasma matrix bone block, and thus maintain space. In addition, the preparation method of the application can effectively improve the degradation performance and cell barrier function of the plasma matrix bone block by heating the surface of the first intermediate after obtaining the first intermediate. The injection of the cell concentrate can maximize the enrichment of effective biological components, improve the local concentration, improve the biological activity of the plasma matrix bone block, and avoid the damage of heating to the effective biological components. Specifically, (1) As for the degradation performance, the degradation of the traditional solid plasma matrix is relatively fast, and it is usually degraded within a few weeks, while the degradation speed of the heated plasma matrix is significantly slowed down and can be maintained for several months, and the core reason lies in the fundamental change of the fibrin network structure. The fibrin network formed by the polymerization of fibrinogen in the traditional plasma matrix is a flexible, loose and spider web-like structure. Although this porous structure is beneficial to cell migration and blood vessel growth, it is more easily recognized and degraded by plasmin, an enzyme that specifically degrades fibrin. The first intermediate is modified by heating in the application, which can denature the protein, and the denatured fibrin molecules will cross-link and aggregate to form a more dense, hard and small-pored fibrin network. This denatured and dense physical structure provides a strong physical barrier to the "attack" of plasmin, and the enzyme molecules are more difficult to penetrate into the material and more difficult to find and bind to the target of their action, thus greatly enhancing the anti-plasmin ability, thereby greatly delaying the degradation process. In short, heating makes the fibrin of the plasma matrix "cooked", which changes from a soft net to a solid wall, and is more resistant to the natural decomposition process of the body, thus degrading more slowly and providing more durable support.

[0008] (2) For the cell barrier function, the improvement and the slow degradation of the barrier function are actually "two sides of the same coin", which are derived from the change of the above structure. After denaturation, the pore size of the dense fibrin network is significantly reduced, which directly physically blocks the passage of certain cells, especially the rapidly proliferating fibroblasts and epithelial cells. The porous structure of the traditional plasma matrix is a "scaffold" that cells like, which can guide and promote the growth of fibroblasts, osteoblasts and other cells into it to achieve tissue regeneration. However, it has poor blocking effect on epithelial cells or connective tissue cells, and in guided bone regeneration that requires space maintenance, they will grow too fast and interfere with the slower bone formation process. However, the first intermediate is heated and modified in the present application, and the dense structure of the heated plasma matrix is no longer a "highway" that cells can easily pass through, but more like a "selective barrier" that can more effectively prevent the rapid invasion of soft tissue, creating a protected and stable space for the bone tissue below that needs longer regeneration. The heating process of the present application realizes the transition from "guided migration" to "blocked migration".

[0009] After the plasma matrix bone block gel is prepared in step (2) of the present application, it can be shaped in a personalized bone graft guide to make the final plasma matrix bone block conform to the three-dimensional morphology of the alveolar process cleft defect area.

[0010] It should be noted that after heating treatment of any surface of the first intermediate in step (2), the surface after heating treatment is used as the surface close to the soft tissue.

[0011] As a preferred technical solution of the present application, the mass to volume ratio of the bone repair material to the whole blood is 0.25 g :(10-60) mL, for example, 0.25 g : 10 mL, 0.25 g : 20 mL, 0.25 g : 30 mL, 0.25 g : 40 mL, 0.25 g : 50 mL, 0.25 g : 60 mL, etc.

[0012] When the mass to volume ratio of the bone repair material and the whole blood is within the above range, the fibrin scaffold in the plasma matrix can effectively stabilize the bone repair material, if the amount of whole blood is too small, the plasma matrix bone block cannot form a dense barrier layer on the surface after heating; if the amount of whole blood is too much, the lack of bone repair material will significantly reduce the overall strength of the plasma matrix bone block.

[0013] As a preferred technical solution of the present application, the bone repair material includes any one or more of autologous bone, allogeneic bone, xenogeneic bone and artificial bone.

[0014] As a preferred technical solution of the present application, the temperature of the heating treatment is 70-95℃, for example, 70℃, 75℃, 80℃, 85℃, 90℃, 95℃, etc.

[0015] As a preferred technical solution of the present application, the time of the heating treatment is 5s-15min, for example, 5s, 30s, 1min, 5min, 10min, 15min, etc.

[0016] When the temperature and time of the heating treatment are within the above ranges, the degradation performance and cell barrier function of the plasma matrix bone block can be more effectively improved. If the heating temperature is too low, an effective dense fibrin network cannot be formed, and if the heating temperature is too high, the fiber may be dehydrated, the brittleness may be increased, and the fibrin structure may be damaged.

[0017] As a preferred technical solution of the present application, the method for hydrophilic treatment of the bone repair material is to use oxygen for vacuum plasma treatment of the bone repair material.

[0018] The present application uses oxygen to perform vacuum plasma treatment on the bone repair material, which can effectively improve the hydrophilicity of the bone repair material, thereby improving the interaction between the bone repair material and the plasma matrix, promoting the cross-linking of fibrin, and forming a more stable structure between the two.

[0019] As a preferred technical solution of the present application, the conditions for vacuum plasma treatment using oxygen include: a gas flow of 0.5-2L / min, a treatment time of 3-600s, and a power of 100-500W, wherein the 0.5-2L / min can be 0.5L / min, 1L / min, 1.5L / min, 2L / min, etc., the 3-600s can be 3s, 60s, 120s, 180s, 240s, 300s, 360s, 420s, 480s, 540s, 600s, etc., and the 100-500W can be 100W, 200W, 300W, 400W, 500W, etc.

[0020] As a preferred technical solution of the present application, the method for hydrophilic treatment of the tube for preparing the solid plasma matrix is to clean the tube for preparing the solid plasma matrix with pure water and then dry it, and then use argon and oxygen for vacuum plasma treatment in sequence.

[0021] The solid plasma matrix preparation tube is sequentially subjected to vacuum plasma treatment with argon and oxygen, which can effectively improve the hydrophilicity of the solid plasma matrix preparation tube, thereby promoting the cross-linking of fibrin between the bone repair material and the plasma matrix, and making the two form a more stable structure.

[0022] In the vacuum plasma treatment of the solid plasma matrix preparation tube with argon and oxygen, the solid plasma matrix preparation tube is controlled to have an inclination angle of 0-9° and is rotated along the central axis.

[0023] As a preferred technical solution of the present application, the gas flow for vacuum plasma treatment with argon is 0.5-2 L / min, the treatment time is 3-600 s, and the power is 100-500 W; wherein the 0.5-2 L / min can be 0.5 L / min, 1 L / min, 1.5 L / min, 2 L / min, etc., the 3-600 s can be 3 s, 60 s, 120 s, 180 s, 240 s, 300 s, 360 s, 420 s, 480 s, 540 s, 600 s, etc., and the 100-500 W can be 100 W, 200 W, 300 W, 400 W, 500 W, etc.

[0024] As a preferred technical solution of the present application, the gas flow for vacuum plasma treatment with oxygen is 0.5-2 L / min, the treatment time is 3-600 s, and the power is 100-500 W; wherein the 0.5-2 L / min can be 0.5 L / min, 1 L / min, 1.5 L / min, 2 L / min, etc., the 3-600 s can be 3 s, 60 s, 120 s, 180 s, 240 s, 300 s, 360 s, 420 s, 480 s, 540 s, 600 s, etc., and the 100-500 W can be 100 W, 200 W, 300 W, 400 W, 500 W, etc.

[0025] As a preferred technical solution of the present application, the centrifugal force of the first centrifugation is 50-700 g, such as 50 g, 100 g, 200 g, 400 g, 600 g, 700 g, etc.

[0026] As a preferred technical solution of the present application, the centrifugal force of the first centrifugation is 1000-3000 g, for example, 1000 g, 1500 g, 2000 g, 2500 g, 3000 g, etc.

[0027] The present application limits the centrifugal force and time of the first centrifugation in the above range, which can more effectively separate the mixed liquid of the liquid plasma matrix and the leukocyte-rich intermediate layer from the whole blood.

[0028] As a preferred technical solution of the present application, the centrifugal force of the second centrifugation is 1000-3000 g, for example, 1000 g, 1500 g, 2000 g, 2500 g, 3000 g, etc.

[0029] As a preferred technical solution of the present application, the time of the second centrifugation is 3-10 min, for example, 3 min, 5 min, 8 min, 10 min, etc.

[0030] The present application limits the centrifugal force and time of the second centrifugation in the above range, which can more effectively separate the upper cell-poor plasma matrix and the lower cell concentrate.

[0031] In a second aspect, the present application provides a plasma matrix bone block prepared by the preparation method of the first aspect.

[0032] In a third aspect, the present application provides an application of the plasma matrix bone block of the second aspect in alveolar process cleft repair.

[0033] The technical solution provided by the embodiments of the present application has the following advantages compared with the prior art: 1. The preparation method provided by the present application mixes the cell-poor plasma matrix with the bone repair material to prepare the plasma matrix bone block, and densifies the three-dimensional fibrin network structure by surface heating, which effectively improves the degradation performance and cell barrier function of the plasma matrix bone block. At the same time, by injecting the cell concentrate rich in effective biological components, the finally prepared plasma matrix bone block has excellent biological activity.

[0034] 2. When the plasma matrix bone block prepared by the present application is applied to the repair treatment of the alveolar process cleft, it can avoid the invasion of soft tissue cells into the bone regeneration area, maintain the stability of the regeneration space, and delay the absorption of the implanted material. Compared with autologous bone transplantation, it can avoid opening a second operation area, and does not need to consider the problem of insufficient source, avoiding the disadvantages of autologous bone transplantation. BRIEF DESCRIPTION OF DRAWINGS

[0035] The accompanying drawings, which are incorporated into and form part of the specification, illustrate embodiments consistent with the present application and, together with the specification, serve to explain the principles of the present application.

[0036] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, for those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0037] Figure 1 The histological HE staining results are shown in the example of the present invention after rabbit alveolar cleft bone regeneration surgery using the plasma matrix bone block provided in Example 2. Detailed Implementation

[0038] To better understand the above-mentioned objectives, features, and advantages of the present invention, the solutions of the present invention will be further described below. It should be noted that, unless otherwise specified, the embodiments of the present invention and the features thereof can be combined with each other.

[0039] Many specific details are set forth in the following description in order to provide a full understanding of the invention, but the invention may also be practiced in other ways different from those described herein; obviously, the embodiments in the specification are only some embodiments of the invention, and not all embodiments.

[0040] Example 1 This embodiment provides a plasma matrix bone block and its preparation method, the preparation method including the following steps: (1) The tube for preparing solid plasma matrix was washed and dried with purified water and ultrapure water in sequence. Then, it was treated with argon gas in a vacuum plasma with a gas flow rate of 1 L / min, a treatment time of 300 s, and a power of 300 W. Then, it was treated with oxygen in a vacuum plasma with a gas flow rate of 1 L / min, a treatment time of 300 s, and a power of 300 W. During the vacuum plasma treatment, the tilt angle of the tube for preparing solid plasma matrix was controlled at 0-9° and rotated along the central axis to obtain the tube for preparing solid plasma matrix after hydrophilic treatment. (2) 0.05 g of allogeneic bone (Bio-Gene) bone repair material was treated with oxygen in a vacuum plasma process at a flow rate of 1 L / min, a treatment time of 300 s, and a power of 300 W to obtain the hydrophilic bone repair material. (3) Collect 6 mL of whole blood and place it in a superhydrophobic liquid plasma matrix preparation tube (without anticoagulant). Perform the first centrifugation at a centrifugation force of 400 g and a centrifugation time of 20 min to obtain a mixture of liquid plasma matrix and leukocyte-rich intermediate layer. Collect the mixture into a new superhydrophobic liquid plasma matrix preparation tube for a second centrifugation at a centrifugation force of 2000 g and a centrifugation time of 6 min to obtain the upper cell-poor plasma matrix and the lower cell concentrate. (4) The bone repair material after hydrophilic treatment in step (2) and the cell-poor plasma matrix obtained in step (3) are placed in the tube for preparing the solid plasma matrix after hydrophilic treatment in step (1) and mixed to obtain plasma matrix bone block gel. The gel is placed in a personalized bone graft guide plate and shaped to obtain the first intermediate. Any surface of the first intermediate is heated at 80°C for 5 min to obtain the second intermediate. (5) Using a disposable syringe, extract the cell concentrate obtained in step (3) and inject it evenly into the second intermediate to obtain the plasma matrix bone block.

[0041] Example 2 This embodiment provides a plasma matrix bone block and its preparation method, the preparation method including the following steps: (1) The tube for preparing solid plasma matrix was washed and dried with purified water and ultrapure water in sequence. Then, it was treated with argon gas in a vacuum plasma with a gas flow rate of 0.5 L / min, a treatment time of 600 s, and a power of 100 W. Then, it was treated with oxygen in a vacuum plasma with a gas flow rate of 0.5 L / min, a treatment time of 600 s, and a power of 100 W. During the vacuum plasma treatment, the tilt angle of the tube for preparing solid plasma matrix was controlled to be 0-9° and rotated along the central axis to obtain the tube for preparing solid plasma matrix after hydrophilic treatment. (2) 0.25 g of xenogeneic bone (Geistlich Bio-Oss) ® The bone repair material was treated with oxygen in a vacuum plasma process at a flow rate of 0.5 L / min, a treatment time of 600 s, and a power of 100 W to obtain a hydrophilic bone repair material. (3) Collect 10 mL of whole blood and place it in a superhydrophobic liquid plasma matrix preparation tube (without anticoagulant). Perform the first centrifugation at a centrifugation force of 50 g and a centrifugation time of 30 min to obtain a mixture of liquid plasma matrix and leukocyte-rich intermediate layer. Collect the mixture into a new superhydrophobic liquid plasma matrix preparation tube for a second centrifugation at a centrifugation force of 1000 g and a centrifugation time of 10 min to obtain the upper cell-poor plasma matrix and the lower cell concentrate. (4) The bone repair material after hydrophilic treatment in step (2) and the cell-poor plasma matrix obtained in step (3) are placed in the tube for preparing the solid plasma matrix after hydrophilic treatment in step (1) and mixed to obtain plasma matrix bone block gel. The gel is placed in a personalized bone graft guide plate and shaped to obtain the first intermediate. Any surface of the first intermediate is heated at 95°C for 5 s to obtain the second intermediate. (5) Using a disposable syringe, extract the cell concentrate obtained in step (3) and inject it evenly into the second intermediate to obtain the plasma matrix bone block.

[0042] Example 3 This embodiment provides a plasma matrix bone block and its preparation method, the preparation method including the following steps: (1) The tube for preparing solid plasma matrix was washed and dried with purified water and ultrapure water in sequence. Then, it was treated with argon gas in a vacuum plasma with a gas flow rate of 2 L / min, a treatment time of 3 s, and a power of 500 W. Then, it was treated with oxygen in a vacuum plasma with a gas flow rate of 2 L / min, a treatment time of 3 s, and a power of 500 W. During the vacuum plasma treatment, the tilt angle of the tube for preparing solid plasma matrix was controlled at 0-9° and rotated along the central axis to obtain the tube for preparing solid plasma matrix after hydrophilic treatment. (2) 0.05 g of artificial bone repair material (biphasic calcium phosphate) was treated with oxygen in a vacuum plasma with a gas flow rate of 2 L / min, a treatment time of 3 s, and a power of 500 W to obtain the hydrophilic bone repair material. (3) Collect 12 mL of whole blood and place it in a superhydrophobic liquid plasma matrix preparation tube (without anticoagulant). Perform the first centrifugation at a centrifugation force of 700 g and a centrifugation time of 5 min to obtain a mixture of liquid plasma matrix and leukocyte-rich intermediate layer. Collect the mixture into a new superhydrophobic liquid plasma matrix preparation tube for a second centrifugation at a centrifugation force of 3000 g and a centrifugation time of 3 min to obtain an upper layer of cell-poor plasma matrix and a lower layer of cell concentrate. (4) The bone repair material after hydrophilic treatment in step (2) and the cell-poor plasma matrix obtained in step (3) are placed in the tube for preparing the solid plasma matrix after hydrophilic treatment in step (1) and mixed to obtain plasma matrix bone block gel. The gel is placed in a personalized bone graft guide plate and shaped to obtain the first intermediate. Any surface of the first intermediate is heated at 70°C for 15 min to obtain the second intermediate. (5) Using a disposable syringe, extract the cell concentrate obtained in step (3) and inject it evenly into the second intermediate to obtain the plasma matrix bone block.

[0043] Application examples This application example utilizes the plasma matrix bone block provided in Example 2 for rabbit alveolar cleft bone regeneration surgery: (1) Establishment of rabbit alveolar cleft model: After anesthetizing the experimental animals with 1% sodium pentobarbital (1 mL / kg) via intravenous injection in the ear, the oral cavity and surrounding area were disinfected with povidone-iodine, draped, and the gingiva was incised to expose the distal alveolar bone surfaces of the left and right upper central incisors and lateral incisors. The alveolar bone was ground away from the distal central and lateral incisors with a dental drill to form a wedge-shaped alveolar cleft defect of approximately 1.0 × 0.5 cm, 1.0 cm from the incisal edge of the lateral incisor. The wound was sutured to form a bilateral complete alveolar cleft animal model. After the rabbit alveolar cleft wounds healed, subsequent surgeries were performed.

[0044] (2) The alveolar cleft defect area was exposed according to the standard surgical procedure, and the plasma matrix bone block prepared in Example 2 was implanted and shaped locally to fit. Soft tissue treatment was performed, and the area was sutured. Antibiotics were administered for 3 days postoperatively, and the patient was observed and treated regularly.

[0045] (3) Specimen Collection and Processing: Experimental animals were euthanized 12 weeks after transplantation by injecting an excessive amount of sodium pentobarbital. The animal skulls (including the maxilla and nasal bones, etc.) were removed, soft tissues were excised, and the alveolar bone thickness in the anterior maxillary transplantation area was directly measured using calipers. The specimens were then rinsed with physiological saline, and the tissue specimens were fixed in 4% paraformaldehyde for 24 hours. After decalcification and graded dehydration with alcohol, sections were prepared and stained with hematoxylin and eosin (HE). The results were observed under a microscope as follows: Figure 1 As shown.

[0046] It can be observed that when the plasma matrix bone block prepared using the present invention is used to repair alveolar clefts in rabbits, the regeneration thickness is 4.16 ± 0.95 mm, which shows good regeneration effect.

[0047] It should be noted that, in this document, relational terms such as "first" and "second" are used merely to distinguish one entity or operation from another, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Furthermore, the terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such a process, method, article, or apparatus. Without further limitations, an element defined by the phrase "comprising one..." does not exclude the presence of other identical elements in the process, method, article, or apparatus that includes said element.

[0048] The above description is merely a specific embodiment of the present invention, enabling those skilled in the art to understand or implement the invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the invention. Therefore, the present invention is not to be limited to the embodiments described herein, but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. A method for preparing a plasma matrix bone block, characterized in that, The preparation method includes: (1) Collect whole blood and centrifuge it for the first time to obtain a mixture of liquid plasma matrix and leukocyte-rich intermediate layer. Centrifuge the mixture for the second time to obtain an upper layer of cell-poor plasma matrix and a lower layer of cell concentrate. (2) The hydrophilically treated bone repair material and the cell-poor plasma matrix are placed in a tube for preparing a hydrophilically treated solid plasma matrix and mixed to obtain a plasma matrix bone block gel. The gel is shaped to obtain a first intermediate. Any surface of the first intermediate is heated to obtain a second intermediate. (3) The cell concentrate is uniformly injected into the second intermediate to obtain the plasma matrix bone block.

2. The preparation method according to claim 1, characterized in that, The mass ratio of the bone repair material to the volume of the whole blood is 0.25 g : (10 - 60) mL.

3. The preparation method according to claim 1 or 2, characterized in that, The bone repair material includes any one or more of autologous bone, allogeneic bone, xenogeneic bone, and artificial bone.

4. The preparation method according to any one of claims 1-3, characterized in that, The temperature of the heat treatment is 70-95℃; And / or, the heat treatment time is 5 s - 15 min.

5. The preparation method according to any one of claims 1-4, characterized in that, The method for hydrophilic treatment of the bone repair material is to treat the bone repair material with oxygen in a vacuum plasma. Preferably, the conditions for vacuum plasma treatment using oxygen include: a gas flow rate of 0.5-2 L / min, a treatment time of 3-600 s, and a power of 100-500 W.

6. The preparation method according to any one of claims 1-5, characterized in that, The hydrophilic treatment method for the tube used to prepare the solid plasma matrix is ​​as follows: the tube is cleaned with pure water and dried, and then vacuum plasma treatment is performed sequentially using argon and oxygen. Preferably, the gas flow rate of the vacuum plasma treatment using argon is 0.5-2 L / min, the treatment time is 3-600 s, and the power is 100-500 W; Preferably, the gas flow rate for vacuum plasma treatment using oxygen is 0.5-2 L / min, the treatment time is 3-600 s, and the power is 100-500 W.

7. The preparation method according to any one of claims 1-6, characterized in that, The centrifugal force for the first centrifugation is 50-700 g; And / or, the first centrifugation time is 5-30 min.

8. The preparation method according to any one of claims 1-7, characterized in that, The centrifugal force during the second centrifugation is 1000-3000 g; And / or, the second centrifugation time is 3-10 min.

9. Plasma matrix bone blocks prepared by the preparation method according to any one of claims 1-8.

10. The application of the plasma matrix bone block as described in claim 9 in the repair of alveolar clefts.

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