New application of pithecellobium clypearia extract for improving egg quality and poultry feed additive
By adding monkey earring extract to laying hen feed, the problem of declining egg quality caused by immune degeneration in laying hens during the later stages of egg production was solved, resulting in improvements in yolk color, eggshell thickness, and laying period, with significant social and economic benefits.
Patent Information
- Application Number
- CN202512001428.9
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-12-29
- Publication Date
- 2026-02-13
AI Technical Summary
Modern laying hens experience a decline in immune function and disease resistance in the later stages of egg production, resulting in thinner eggshells, more broken eggs, and reduced egg quality, which negatively impacts the economic benefits of the poultry industry.
Monkey earring extract can be added to poultry feed as an additive at a dosage of 50 mg/kg or 200-3200 mg/kg to improve egg quality.
It significantly improves egg quality, including yolk color, albumen height, and shell thickness, extends the laying period, and enhances the economic benefits of the aquaculture industry.
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Abstract
Description
Technical Field
[0001] This invention relates to the field of biotechnology, specifically to the field of plant extract technology and chicken feed technology. Background Technology
[0002] The rearing of laying hens mainly includes three stages: early laying, mid-laying, and late laying. Late laying generally refers to the period from 43 to 72 weeks of age. Modern laying hens are characterized by high production, a relatively short production cycle, and vigorous metabolism. After the peak laying period, the digestive and metabolic systems of hens gradually weaken due to the high intensity and heavy workload, especially the immune system, leading to decreased disease resistance. In late laying, the weekly egg production rate of the flock decreases by about one percent. The absorption of nutrients also decreases, weight gain stops, and fat gradually accumulates, causing the hens to become obese. Reduced calcium absorption leads to thinner and more brittle eggshells, with a significant increase in broken and soft-shelled eggs. Since late laying accounts for almost half of the entire laying period, this stage directly impacts the economic benefits of laying hen farming. Generally, after 72 weeks of age, the egg production rate drops below 70%, egg quality declines significantly, and farming profits decrease substantially; therefore, gradual culling is recommended. Therefore, if methods and additives can be found and developed to significantly improve egg quality and thus extend the laying period of laying hens, it will have enormous social and economic benefits. Summary of the Invention
[0003] The technical problem to be solved by the present invention is to provide a new use for monkey earring extract, as well as a poultry feed additive that can significantly improve egg quality, and a poultry feed.
[0004] The technical solution of the present invention to solve the above-mentioned technical problems is as follows:
[0005] A novel use of monkey earring extract to improve egg quality.
[0006] The application method involves feeding monkey earring extract to poultry. Specifically, the monkey earring extract can be added to poultry feed. Alternatively, it can be made into an additive and then added to poultry feed for chickens.
[0007] The dosage of monkey earring extract added to poultry feed is 50 mg / kg, or 200-3200 mg / kg. Preferably, the dosage of monkey earring extract added to each kg of poultry feed is 50 mg, 200 mg, 400 mg, 800 mg, 1600 mg, or 3200 mg. It should be noted that since the verification test of this invention used 580-day-old (approximately 82 weeks old, belonging to culled laying hens) Hy-Line Brown laying hens, when the age and breed of the target laying hens for improving egg quality are different, or when applied to improve the egg quality of other types of poultry such as ducks, geese, and quails, the most suitable dosage may vary. Therefore, the dosage of monkey earring extract added in this invention is not limited to the dosage disclosed in the verification test. Regardless of the dosage, as long as monkey earring extract is used to improve the egg quality of poultry, it should be within the scope of this invention.
[0008] A poultry feed additive for improving egg quality contains monkey earring extract.
[0009] The dosage of the additive in poultry feed, based on the weight of the monkey earring extract, is 50 mg / kg or 200-3200 mg / kg. Preferably, the dosage of the additive per kg of poultry feed, based on the weight of the monkey earring extract, is 50 mg, 200 mg, 400 mg, 800 mg, 1600 mg, or 3200 mg of the monkey earring extract. It should be noted that since the verification test of this invention used 580-day-old (approximately 82 weeks old, belonging to culled laying hens) Hy-Line Brown laying hens, when the age and breed of the target laying hens for improving egg quality are different, or when applied to improve the egg quality of other types of poultry such as ducks, geese, and quails, the most suitable dosage may vary. Therefore, the dosage of the monkey earring extract in this invention is not limited to the dosage disclosed in the verification test. Regardless of the dosage, as long as the monkey earring extract is used to improve the egg quality of poultry, it should be within the scope of this invention.
[0010] A poultry feed containing monkey earring extract.
[0011] The amount of monkey earring extract added is 50 mg / kg, or 200-3200 mg / kg. Preferably, the amount of the additive per kg of poultry feed, based on the weight of the monkey earring extract, is 50 mg, 200 mg, 400 mg, 800 mg, 1600 mg, or 3200 mg of the monkey earring extract. It should be noted that since the verification test of this invention used 580-day-old (approximately 82 weeks old, belonging to culled laying hens) Hy-Line Brown laying hens, when the age and breed of the target laying hens for improving egg quality are different, or when applied to improve the egg quality of other types of poultry such as ducks, geese, and quails, the most suitable addition amount may vary. Therefore, the amount of monkey earring extract added in this invention is not limited to the amount disclosed in the verification test. Regardless of the amount added, as long as the monkey earring extract is used to improve the egg quality of poultry, it should be within the scope of this invention.
[0012] Furthermore, the poultry includes any one of chicken, duck, goose, or quail.
[0013] The present invention has the following beneficial effects:
[0014] This invention utilizes monkey earring extract as a poultry feed additive, which can improve egg yolk color, average albumen height, Haugh unit, and average eggshell thickness, thereby improving egg quality and extending the laying period, resulting in significant social and economic benefits. Detailed Implementation
[0015] The present invention will be further described in detail below with reference to specific embodiments. The embodiments given are only for illustrating the present invention and are not intended to limit the scope of the present invention. Unless otherwise specified, the experimental methods in the following embodiments are conventional methods. Unless otherwise specified, the materials, reagents, instruments, etc. used in the following embodiments are all commercially available.
[0016] 1. Test substance
[0017] The test product is named Monkey Earring Extract, and its active ingredients are total polyphenols, gallic acid, and quercetin. It appears as a yellowish-brown powder.
[0018] 2 Materials and Methods
[0019] 2.1 Experimental Animals and Design
[0020] This experiment used 580-day-old Hy-Line Brown laying hens. Based on the principle of no difference in pre-feeding egg production rate, they were randomly divided into 8 treatments, with 8 replicates per treatment and 12 birds per replicate. The feeding period was 56 days (8 weeks). The experimental groups are shown in Table 1.
[0021] Table 1 Experimental Groups
[0022]
[0023] 2.2 Experimental Diets
[0024] The experimental diet was Haida commercial laying hen feed, and its raw material composition and nutritional composition are shown in the table below.
[0025] Table 2. Composition and nutritional components of the experimental diet (air-dried basis)
[0026]
[0027] 1) The premix provides the following per kilogram of feed: Vitamin A 600,000 IU; Vitamin D3 250,000 IU; Vitamin E 2000 mg; Vitamin K3 80 mg; Vitamin B1 100 mg; Vitamin B2 400 mg; Vitamin B6 100 mg; Vitamin B12 1.0 mg; Niacin 1000 mg; Pantothenic acid 500 mg; Folic acid 100 mg; Biotin 10 mg; Vitamin C 1000 mg; Choline chloride 300 mg; Fe (ferrous sulfate) 70 mg; Mn (manganese sulfate) 75 mg; Zn (zinc sulfate) 80 mg; I (calcium iodate) 0.6 mg; Cu (copper sulfate) 8 mg; Se (sodium selenite) 0.3 mg.
[0028] 2.3 Feed Management
[0029] A three-tiered, fully stepped cage rearing system was adopted, with feeding times at 8:00, 11:00, and 18:00 daily. Feeding and watering were free for the participants, and strict adherence to company regulations was maintained, with timely data recording. All other experimental conditions remained consistent throughout the experiment.
[0030] 2.4 Egg quality determination indicators and methods
[0031] After 56 days of rearing, 20 eggs were randomly selected from each group to test egg quality-related indicators, including yolk color, average albumen height, Haugh unit, and average shell thickness. The testing methods for these indicators were well-known in the field and will not be described in detail here.
[0032] 2.5 Data Analysis
[0033] Data were analyzed using SPSS 25.0 software. One-way ANOVA was performed. The results are expressed as mean and standard error. p < 0.05 was considered statistically significant, and p < 0.01 was considered highly statistically significant.
[0034] 3. Experimental Results and Analysis
[0035] Table 3 shows the effects of different doses of monkey earring extract on egg quality in laying hens at 56 days of late laying period.
[0036] Table 3. Effects of different doses of monkey earring extract on egg quality in laying hens at 56 days of late laying period.
[0037]
[0038] Note: Data with the same or no superscript letter in the same row indicates no significant difference (p > 0.05), while data with different letters indicates a significant difference (p < 0.05). Average albumen height: i.e., egg white height.
[0039] Analysis of the yolk color data in the table reveals that the yolk color of eggs from the 50 mg / kg and 200 mg / kg~3200 mg / kg dose groups of monkey earring extract was significantly higher than that of the blank control. This indicates that these dose groups can significantly improve the yolk color of eggs laid by the tested hens. A higher yolk color value indicates higher nutritional value and egg quality. Furthermore, the yolk color value of the 100 mg / kg dose group was significantly lower than that of the blank control; therefore, 100 mg / kg is a dose point that should be avoided.
[0040] Analysis of the average albumen height data in the table reveals that the monkey earring extract dosage groups (50 mg / kg, 200 mg / kg~3200 mg / kg) were significantly higher than the blank control group (Contorl). This indicates that these dosage groups can significantly increase the average albumen height of eggs produced by the tested hens. A higher average albumen height indicates a thicker, firmer egg white and a fresher egg. Furthermore, the average albumen height of the 100 mg / kg dosage group in the table was significantly lower than the blank control; therefore, 100 mg / kg is a dosage point that should be avoided.
[0041] Analysis of the Hastings unit data in the table reveals that the Hastings units in all seven dose groups of monkey earring extract (50–3200 mg / kg) were higher than those in the control group (Contorl). The Hastings unit is a core quantitative indicator for measuring egg freshness, primarily reflecting egg quality by detecting the viscosity and height of the egg white (protein). This indicates that adding monkey earring extract to laying hen feed is beneficial for improving egg freshness, thereby enhancing egg quality.
[0042] Analysis of the average eggshell thickness data in the table shows that the average eggshell thickness of all seven monkey earring extract dosage groups (50-3200 mg / kg) was significantly higher than that of the blank control group (Contorl). This indicates that adding 50-3200 mg / kg monkey earring feed additive to laying hen feed can significantly improve the strength of eggshells, that is, make them more resistant to breakage, less likely to crack or develop fissures during collection, sorting, packaging, transportation and sales, and less susceptible to bacterial contamination (such as Salmonella).
[0043] 4. Conclusion
[0044] Monkey earring extract has a significant impact on egg quality. During a 56-day study, different doses of monkey earring extract showed a significant effect on egg quality in laying hens, specifically on indicators such as yolk color, average albumen height, Haugh unit, and average shell thickness. These results indicate that monkey earring extract can improve egg quality within a certain dosage range, but the effects vary with different doses, requiring further optimization to achieve the best results.
Claims
1. A new use of monkey earring extract to improve egg quality.
2. The novel application according to claim 1, characterized in that: The application method involves feeding monkey earring extract to poultry.
3. The novel application according to claim 2, characterized in that: Monkey earring extract can be added to poultry feed for poultry consumption, or it can be made into an additive and then added to poultry feed for poultry consumption.
4. The novel application according to claim 3, characterized in that: The dosage of monkey earring extract added to the poultry feed is 50 mg / kg or 200~3200 mg / kg.
5. A poultry feed additive for improving egg quality, characterized in that: It contains monkey earring extract.
6. The poultry feed additive according to claim 5, characterized in that: The dosage added to each kg of poultry feed is 50 mg or 200-3200 mg based on the weight of the monkey earring extract.
7. The poultry feed additive according to claim 6, characterized in that: The dosage added to each kg of poultry feed is 50 mg, 200 mg, 400 mg, 800 mg, 1600 mg, or 3200 mg, based on the weight of the monkey earring extract.
8. A poultry feed containing monkey earring extract.
9. The chicken feed according to claim 8, characterized in that: The amount of monkey earring extract added is 50 mg / kg, or 200~3200 mg / kg.
10. The novel use according to any one of claims 1 to 4, or the poultry feed additive according to any one of claims 5 to 7, or the poultry feed according to any one of claims 8 to 9, characterized in that, The poultry includes any one of chickens, ducks, geese, and quails.