Antioxidant and anti-aging skin repair composition and preparation method thereof

Abstract

The invention relates to the technical field of cosmetics, and particularly discloses an anti-oxidation and anti-aging skin repair composition and a preparation method thereof, the anti-oxidation and anti-aging skin repair composition comprises pterostilbene, ergothioneine, a repair soothing component, a plant fermentation extract, Ectoine, a composite antioxidant, polyol, a surfactant, and the balance purified water. The preparation method comprises the following steps: step 1, adding polyhydric alcohol and Ectoine into purified water to obtain a water phase; adding a surfactant into the composite antioxidant, and uniformly stirring to obtain an oil phase; pumping the oil phase into the water phase, and uniformly stirring at the temperature of 60-65 DEG C to obtain an emulsion; and step 2, homogenizing the emulsion, adding a repairing and relieving component, cooling, adding pterostilbene, ergothioneine and a plant fermentation extract, adjusting the pH value, and filtering to obtain the antioxidant and anti-aging skin repairing composition which has the effects of long-acting moisturizing, skin smoothing, tightening and elastic moistening.

Description

Technical Field

[0001] This application relates to the field of cosmetic technology, and more specifically, to an antioxidant and anti-aging skin repair composition and a method for preparing the same. Background Technology

[0002] In the field of cosmetic skin care, skin is susceptible to factors such as ultraviolet radiation, environmental pollution, and aging, which can lead to excessive free radicals causing oxidative damage, resulting in collagen loss, decreased elasticity, and impaired barrier function, leading to aging and repair needs such as fine lines, sensitivity, and dryness. Existing antioxidant and anti-aging products often focus on a single effect or achieve basic care through simple compounding of active ingredients, resulting in poor synergy of effects and low bioavailability of active ingredients. For example, some products rely on chemical antioxidants, which can easily cause skin irritation; plant extracts are mostly extracted using traditional solvents, making it difficult for large molecules to be absorbed by the skin, and their activity has not been enhanced through optimized fermentation processes; the proportions of repairing ingredients are unbalanced, making it difficult to simultaneously meet the integrated needs of immediate soothing, long-lasting anti-aging, and oxidative protection. Therefore, there is an urgent need to develop a composition with gentle ingredients and synergistic effects, achieving highly effective antioxidant, anti-aging, and skin repair functions through scientific compounding of active ingredients and optimized preparation processes. This would address the technical shortcomings of existing products and meet consumers' demand for multifunctional and highly safe skin care products. Summary of the Invention

[0003] To address the technical problems mentioned in the background art, this application provides an antioxidant and anti-aging skin repair composition and its preparation method.

[0004] An antioxidant and anti-aging skin repair composition, comprising the following components by weight percentage: 0.1-0.3% pterostilbene, 0.05-0.5% ergothioneine, 0.15-0.6% repair and soothing ingredients, 1.0-3.0% plant fermentation extract, 0.2-0.5% ectoine, 0.2-1.2% compound antioxidant, 0.1-0.3% polyol, 0.05-0.5% surfactant, and the balance being purified water.

[0005] Preferably, the repairing and soothing ingredients include hyaluronic acid peptides, palmitoyl tripeptide-5, hexapeptide-9, palmitoyl tetrapeptide-7, carnosine, and acetyl tetrapeptide-11.

[0006] Preferably, the content of each component in the repairing and soothing ingredient, based on the weight percentage of the composition, is as follows: Hyaluronic acid peptides: 0.03-0.12%; Palmitoyl tripeptide-5: 0.02-0.09%; Hexapeptide-9: 0.02-0.07%; Palmitoyl tetrapeptide-7: 0.02-0.08%; Carnosine: 0.04-0.17%; Acetyl tetrapeptide-11: 0.02-0.07%.

[0007] Preferably, the preparation of the plant fermentation extract includes the following preparation steps: S1. Remove impurities from green tea, white fungus, rose and licorice, wash them, dry them until the moisture content is ≤8%, crush them, sieve them and mix them to obtain a mixture; S2. Add purified water to the mixture, stir evenly, adjust the pH to 6.0-6.5, sterilize, and cool to 28-32℃ to obtain plant slurry; inoculate Aspergillus oryzae into the plant slurry, stir evenly, and ferment for 48-72 hours at a fermentation temperature of 28-32℃ and an aeration rate of 0.5-1.0L / min to obtain the first fermentation product; S3. Add purified water to the first fermentation product, adjust the solid-liquid ratio to 1:5-8, stir evenly, sterilize at 115℃ for 15-20 min, cool to 25-30℃, inoculate the adjusted system with compound bacteria, and ferment at 25-30℃ for 36-48 h to obtain the second fermentation product. S4. The second fermentation product is extracted by ultrasound, filtered to remove residue, and the filtrate is collected. Under vacuum conditions of 0.08-0.1 MPa and 50-60℃, the filtrate is concentrated to a solid content of 15-20% to obtain the plant fermentation extract.

[0008] Preferably, the mass ratio of green tea, white fungus, rose and licorice in step S1 is 10-20:5-12:20-30:10-20.

[0009] Preferably, the inoculum amount of Aspergillus oryzae in step S2 is 3-5% of the mass of the plant slurry.

[0010] Preferably, in step S3, the compound bacteria consists of *Lactobacillus plantarum* and *Saccharomyces cerevisiae* with an effective live bacteria count of 8-12:3-5.

[0011] Preferably, the inoculation amount of the compound bacteria in step S3 is 1.5-3.2% of the adjusted system mass.

[0012] Preferably, the composite antioxidant is composed of oryzanol, vitamin E, and phytosterols in a mass ratio of 10-18:5-8:10-12.

[0013] Preferably, the polyol is one or more of propylene glycol, 1,3-propanediol, glycerol, diglycerol, butylene glycol, pentanediol, and hexanediol.

[0014] Preferably, the surfactant is lecithin and / or PPG-13-decyltetradecyl alcohol polyether-24.

[0015] A method for preparing an antioxidant and anti-aging skin repair composition includes the following preparation steps: Step 1: Add polyol to purified water, heat to 65-70℃, stir for 10-15 min, add ectoine, and continue stirring for 8-10 min to obtain an aqueous phase; add surfactant to the composite antioxidant, stir at 40-45℃ for 10-15 min to obtain an oil phase; pump the oil phase into the aqueous phase, controlling the pumping rate at 5-8 mL / min, maintain the temperature at 60-65℃ at a stirring speed of 1500-2000 rpm, and stir for 20-25 min to obtain an emulsion; Step 2: Homogenize the emulsion at a pressure of 20-25 MPa for 5-8 minutes and a temperature of 55-60°C. Add the repairing and soothing ingredients and stir for 15-20 minutes. Continue cooling to 38-40°C, then add pterostilbene, ergothioneine, and plant fermentation extracts. Stir for 10-15 minutes, cool to below 30°C, adjust the pH to 5.5-6.5, stir for 5-8 minutes, and filter to obtain an antioxidant and anti-aging skin repair composition.

[0016] In summary, this application has the following beneficial effects: This application utilizes a complex blend of six core ingredients for repair and soothing: hyaluronic acid peptides, palmitoyl tripeptide-5, hexapeptide-9, palmitoyl tetrapeptide-7, carnosine, and acetyl tetrapeptide-11. This multi-target synergistic effect ensures gentle performance suitable for various skin types, providing comprehensive functional coverage without any shortcomings in repair. Hyaluronic acid peptides combine moisturizing and penetrating advantages, quickly replenishing and locking in moisture to build a hydrated skin base, while also optimizing the skin microenvironment and significantly improving the penetration efficiency of other active ingredients. Palmitoyl tripeptide-5, as a targeted anti-aging ingredient, precisely activates fibroblasts, promoting the synthesis of type I and type III collagen, specifically improving fine lines and sagging caused by collagen loss. Hexapeptide-9 focuses on repairing the skin's basement membrane, strengthening the connection stability between the epidermis and dermis, reducing damage to the skin structure from external stimuli, and lowering the risk of sensitivity and redness. Palmitoyl tetrapeptide-7... By inhibiting the release of pro-inflammatory factors such as IL-6 and TNF-α, it effectively alleviates skin inflammation induced by environmental stimuli or ultraviolet radiation, creating a non-inflammatory environment for repair and anti-aging. Carnosine simultaneously exerts a dual effect of anti-oxidation and anti-glycation, both clearing free radicals to assist the antioxidant system and inhibiting the deposition of glycation products, preventing dull and stiff skin, while protecting collagen and peptides from oxidative degradation. Acetyl tetrapeptide-11 focuses on stratum corneum lipid synthesis, promoting the production of ceramides and cholesterol, filling the lipid gap in the skin barrier, improving dryness and flaking, and making the skin barrier more resilient. The six ingredients form a progressive mechanism of action of "moisturizing-repairing-anti-aging". Hyaluronic acid peptides first pave the absorption channels, then the barrier repair ingredients strengthen the foundation, and finally the anti-aging ingredients achieve the core efficacy. The efficacy far exceeds that of a single ingredient or simple combination, reducing skin damage from the source and enhancing the anti-aging effect. In addition, carnosine and hyaluronic acid peptides can encapsulate and protect peptide ingredients, preventing them from becoming ineffective during storage or application, thus extending the overall duration of efficacy. Furthermore, they form a secondary synergistic effect with ingredients such as pterostilbene and ectoine in the formula, further enhancing antioxidant and repair capabilities.

[0017] This application utilizes Aspergillus oryzae to ferment a mixture of green tea, tremella, rose, and licorice. The cellulase and protease secreted by Aspergillus oryzae decompose large-molecule polysaccharides and proteins in the plant materials, releasing active ingredients such as tea polyphenols, tremella polysaccharides, rose flavonoids, and glycyrrhizic acid from their cell walls and converting them into easily absorbed small molecules, thus increasing the dissolution rate and bioavailability of the active ingredients. A secondary fermentation is then carried out using Lactobacillus plantarum and Saccharomyces cerevisiae. Lactobacillus plantarum produces organic acids to regulate the pH of the system and inhibit the growth of other microorganisms, while Saccharomyces cerevisiae further transforms the previous fermentation products into new active substances such as small-molecule peptides and amino acids. This results in a richer composition of the fermentation products, enhancing their antioxidant and soothing effects, reducing the potential allergenicity of the plant materials, and improving skin tolerance. Detailed Implementation

[0018] The present application will be further described in detail below with reference to the embodiments.

[0019] Hyaluronic acid peptides and palmitoyl tripeptide-5 (model: SYN-HYCAN) used in the embodiments and comparative examples of this application were purchased from Guangzhou Jinbaolai Biotechnology Co., Ltd.; hexapeptide-9 and palmitoyl tetrapeptide-7 were purchased from Hantai Biopharmaceutical Group Co., Ltd.; carnosine was purchased from Jiangsu Weizhirun Biotechnology Co., Ltd.; acetyl tetrapeptide-11 was purchased from Shaanxi Wensheng Biotechnology Co., Ltd.; Aspergillus oryzae (product name: B89762), Lactobacillus plantarum (product name: B94796), and Saccharomyces cerevisiae (product name: B98025) were all purchased from Ningbo Mingzhou Biotechnology Co., Ltd.

[0020] Examples 1-3 provide an antioxidant and anti-aging skin repair composition and its preparation method.

[0021] Example 1 An antioxidant and anti-aging skin repair composition, by weight percentage, comprises the following components: pterostilbene 0.1%, ergothioneine 0.05%, repair and soothing ingredients 0.15%, plant fermentation extract 1.0%, ectoine 0.2%, complex antioxidant 0.2%, polyol 0.1%, surfactant 0.05%, and the balance being purified water. Specifically, by weight percentage, the contents of each component in the repair and soothing ingredients are as follows: hyaluronic acid peptide: 0.03%; palmitoyl tripeptide-5: 0.02%; hexapeptide-9: 0.02%; palmitoyl tetrapeptide-7: 0.02%; carnosine: 0.04%; acetyl tetrapeptide-11: 0.02%; the complex antioxidant is composed of oryzanol, vitamin E, and phytosterols in a mass ratio of 10:5:10; the polyol is propylene glycol; and the surfactant is PPG-13-decyltetradecyl alcohol polyether-24.

[0022] The preparation of plant fermentation extracts includes the following steps: S1. Remove impurities from green tea, white fungus, rose and licorice, wash them three times with deionized water, dry them until the moisture content is 8%, pulverize them, pass them through an 80-mesh sieve, and mix them to obtain a mixture in which the mass ratio of green tea, white fungus, rose and licorice is 10:5:20:10. S2. Add purified water to the mixture, adjust the solid-liquid ratio to 1:8, stir at 800 rpm until homogeneous, adjust the pH to 6.0, sterilize at 121℃ for 20 min, and cool to 28℃ to obtain plant slurry; inoculate the plant slurry with Aspergillus oryzae, controlling the inoculation amount to 3% of the plant slurry mass, stir at 500 rpm until homogeneous, and ferment at 28℃ and aeration rate of 0.5 L / min for 48 h to obtain the first fermentation product; S3. Add purified water to the first fermentation product, adjust the solid-liquid ratio to 1:5, stir at 800 rpm until homogeneous, sterilize at 115℃ for 15 min, cool to 25℃, inoculate the adjusted system with compound bacteria, and ferment at 25℃ for 36 h to obtain the second fermentation product. The compound bacteria consist of Lactobacillus plantarum and Saccharomyces cerevisiae with an effective viable count of 8:3, and the inoculation amount of the compound bacteria is 1.5% of the mass of the adjusted system. S4. The second fermentation product is ultrasonically extracted for 30 minutes at a power of 300W and a temperature of 50℃. The residue is removed by filtration, and the filtrate is collected and concentrated under reduced pressure at a vacuum of 0.08MPa and a temperature of 50℃ until the solid content is 15% to obtain the plant fermentation extract.

[0023] A method for preparing an antioxidant and anti-aging skin repair composition includes the following preparation steps: Step 1: Add polyol to purified water, heat to 65℃, stir at 500 rpm for 10 min, add ectoine, and continue stirring for 8 min to obtain the aqueous phase; add surfactant to the composite antioxidant, stir at 40℃ and 500 rpm for 10 min to obtain the oil phase; pump the oil phase into the aqueous phase, controlling the pumping rate at 5 mL / min, and stir at 1500 rpm while maintaining the temperature at 60℃ for 20 min to obtain the emulsion; Step 2: Homogenize the emulsion at a pressure of 20 MPa for 5 minutes. Add the repairing and soothing ingredients and stir at 800 rpm for 15 minutes. Continue cooling to 38°C, then add pterostilbene, ergothioneine, and plant fermentation extracts. Stir at 1000 rpm for 10 minutes. Cool to 25°C, adjust the pH to 5.5, and continue stirring for 5 minutes. Filter to obtain an antioxidant and anti-aging skin repair composition.

[0024] Example 2 An antioxidant and anti-aging skin repair composition, by weight percentage, comprises the following components: pterostilbene 0.2%, ergothioneine 0.25%, repair and soothing ingredients 0.4%, plant fermentation extract 2.0%, ectoine 0.3%, complex antioxidant 0.8%, polyol 0.2%, surfactant 0.25%, and the balance being purified water. The contents of each component in the repair and soothing ingredients, by weight percentage, are as follows: hyaluronic acid peptide: 0.08%; palmitoyl tripeptide-5: 0.05%; hexapeptide-9: 0.05%; palmitoyl tetrapeptide-7: 0.05%; carnosine: 0.12%; acetyl tetrapeptide-11: 0.05%. The complex antioxidant is composed of oryzanol, vitamin E, and phytosterols in a mass ratio of 15:6:11. The polyol is propylene glycol. The surfactant is PPG-13-decyltetradecyl alcohol polyether-24.

[0025] The preparation of plant fermentation extracts includes the following steps: S1. Remove impurities from green tea, white fungus, rose and licorice, wash them three times with deionized water, dry them until the moisture content is 5%, pulverize them, pass them through a 100-mesh sieve, and mix them to obtain a mixture in which the mass ratio of green tea, white fungus, rose and licorice is 15:8:25:15. S2. Add purified water to the mixture, adjust the solid-liquid ratio to 1:10, stir at 900 rpm until homogeneous, adjust the pH to 6.2, sterilize at 121℃ for 25 min, and cool to 30℃ to obtain plant slurry; inoculate the plant slurry with Aspergillus oryzae, controlling the inoculation amount of Aspergillus oryzae to 4% of the mass of the plant slurry, stir at 800 rpm until homogeneous, and ferment at 30℃ and a ventilation rate of 0.8 L / min for 60 h to obtain the first fermentation product; S3. Add purified water to the first fermentation product, adjust the solid-liquid ratio to 1:7, stir evenly at 900 rpm, sterilize at 115℃ for 18 min, cool to 28℃, inoculate the adjusted system with compound bacteria, and ferment at 28℃ for 42 h to obtain the second fermentation product. The compound bacteria consist of Lactobacillus plantarum and Saccharomyces cerevisiae with an effective viable count of 10:4, and the inoculation amount of the compound bacteria is 2.5% of the mass of the adjusted system. S4. The second fermentation product was ultrasonically extracted for 38 minutes at a power of 350W and a temperature of 55℃. The residue was removed by filtration, and the filtrate was collected and concentrated under reduced pressure at a vacuum of 0.09MPa and a temperature of 55℃ until the solid content was 18% to obtain the plant fermentation extract.

[0026] A method for preparing an antioxidant and anti-aging skin repair composition includes the following preparation steps: Step 1: Add polyol to purified water, heat to 68℃, stir at 800 rpm for 18 min, add ectoine, and continue stirring for 9 min to obtain the aqueous phase; add surfactant to the composite antioxidant, stir at 42℃ and 800 rpm for 12 min to obtain the oil phase; pump the oil phase into the aqueous phase, controlling the pumping rate at 7 mL / min, and stir at 1800 rpm while maintaining the temperature at 62℃ for 22 min to obtain the emulsion; Step 2: Homogenize the emulsion at a pressure of 22 MPa for 7 minutes. Add the repairing and soothing ingredients and stir at 1000 rpm for 18 minutes. Continue cooling to 39°C, then add pterostilbene, ergothioneine, and plant fermentation extracts. Stir at 1200 rpm for 12 minutes. Cool to 25°C, adjust the pH to 6.0, and continue stirring for 7 minutes. Filter to obtain an antioxidant and anti-aging skin repair composition.

[0027] Example 3 An antioxidant and anti-aging skin repair composition, by weight percentage, comprises the following components: pterostilbene 0.3%, ergothioneine 0.5%, repair and soothing ingredients 0.6%, plant fermentation extract 3.0%, ectoine 0.5%, complex antioxidant 1.2%, polyol 0.3%, surfactant 0.5%, and the balance being purified water. The contents of the repair and soothing ingredients, by weight percentage, are as follows: hyaluronic acid peptide: 0.12%; palmitoyl tripeptide-5: 0.09%; hexapeptide-9: 0.07%; palmitoyl tetrapeptide-7: 0.08%; carnosine: 0.17%; acetyl tetrapeptide-11: 0.07%. The complex antioxidant is composed of oryzanol, vitamin E, and phytosterols in a mass ratio of 18:8:12. The polyol is propylene glycol. The surfactant is PPG-13-decyltetradecyl alcohol polyether-24.

[0028] The preparation of plant fermentation extracts includes the following steps: S1. Remove impurities from green tea, white fungus, rose and licorice, wash them three times with deionized water, dry them until the moisture content is 8%, pulverize them, pass them through a 100-mesh sieve, and mix them to obtain a mixture. The mass ratio of green tea, white fungus, rose and licorice is 20:12:30:20. S2. Add purified water to the mixture, adjust the solid-liquid ratio to 1:12, stir at 1000 rpm until homogeneous, adjust the pH to 6.5, sterilize at 121℃ for 30 min, and cool to 32℃ to obtain plant slurry; inoculate the plant slurry with Aspergillus oryzae, controlling the inoculation amount of Aspergillus oryzae to 5% of the mass of the plant slurry, stir at 1000 rpm until homogeneous, and ferment at 32℃ and a ventilation rate of 1.0 L / min for 72 h to obtain the first fermentation product; S3. Add purified water to the first fermentation product, adjust the solid-liquid ratio to 1:8, stir evenly at 1000 rpm, sterilize at 115℃ for 20 min, cool to 30℃, inoculate the adjusted system with compound bacteria, and ferment at 30℃ for 48 h to obtain the second fermentation product. The compound bacteria consist of Lactobacillus plantarum and Saccharomyces cerevisiae with an effective viable count of 12:5, and the inoculation amount of the compound bacteria is 3.2% of the mass of the adjusted system. S4. The second fermentation product was ultrasonically extracted for 45 minutes at a power of 400W and a temperature of 60℃. The residue was removed by filtration, and the filtrate was collected and concentrated under reduced pressure at a vacuum of 0.1MPa and a temperature of 60℃ until the solid content was 20% to obtain the plant fermentation extract.

[0029] A method for preparing an antioxidant and anti-aging skin repair composition includes the following preparation steps: Step 1: Add polyol to purified water, heat to 70℃, stir at 1000 rpm for 15 min, add ectoine, and continue stirring for 10 min to obtain the aqueous phase; add surfactant to the composite antioxidant, stir at 45℃ and 1000 rpm for 15 min to obtain the oil phase; pump the oil phase into the aqueous phase, controlling the pumping rate at 8 mL / min, and stir at 2000 rpm while maintaining the temperature at 65℃ for 25 min to obtain the emulsion; Step 2: Homogenize the emulsion at a pressure of 25 MPa for 8 minutes. Add the repairing and soothing ingredients and stir at 1200 rpm for 20 minutes. Continue cooling to 40°C, then add pterostilbene, ergothioneine, and plant fermentation extracts. Stir at 1500 rpm for 15 minutes. Cool to 25°C, adjust the pH to 6.5, and continue stirring for 8 minutes. Filter to obtain an antioxidant and anti-aging skin repair composition.

[0030] Comparative Example 1 This comparative example is the same as Example 2, except that the composition method of the repair and soothing ingredients is different. The content of each component in the repair and soothing ingredients by weight percentage is as follows: hyaluronic acid peptide: 0.02%; palmitoyl tripeptide-5: 0.01%; hexapeptide-9: 0.15%; palmitoyl tetrapeptide-7: 0.01%; carnosine: 0.20%; acetyl tetrapeptide-11: 0.01%.

[0031] Comparative Example 2 This comparative example is the same as Example 2, except that the composition method of the repair and soothing ingredients is different. The content of each component in the repair and soothing ingredients by weight percentage is as follows: hyaluronic acid peptide: 0.08%; palmitoyl tripeptide-5: 0.05%; hexapeptide-9: 0.05%; carnosine: 0.12%; sodium hyaluronate: 0.10%.

[0032] Comparative Example 3 This comparative example is the same as Example 2, except that the preparation method of the plant fermentation extract is different, as follows: The preparation of plant fermentation extracts includes the following steps: S1. Remove impurities from green tea, white fungus, rose and licorice, wash them three times with deionized water, dry them until the moisture content is 5%, pulverize them, pass them through a 100-mesh sieve, and mix them to obtain a mixture in which the mass ratio of green tea, white fungus, rose and licorice is 15:8:25:15. S2. Add purified water to the mixture, adjust the solid-liquid ratio to 1:10, stir evenly at 900 rpm, adjust the pH to 6.2, sterilize at 121℃ for 25 min, and cool to 30℃ to obtain plant slurry; inoculate the plant slurry with compound bacteria, controlling the inoculation amount of compound bacteria to 2.5% of the mass of plant slurry, ferment at 28℃ for 102 h to obtain the first fermentation product, wherein the compound bacteria consists of Lactobacillus plantarum and Saccharomyces cerevisiae with an effective viable count of 10:4, and the inoculation amount of compound bacteria is 2.5% of the adjusted system mass; S3. The first fermentation product was ultrasonically extracted for 38 minutes at a power of 350W and a temperature of 55℃. The residue was removed by filtration, and the filtrate was collected and concentrated under reduced pressure at a vacuum of 0.09MPa and a temperature of 55℃ until the solid content was 18% to obtain the plant fermentation extract.

[0033] Comparative Example 4 This comparative example is the same as Example 2, except that the preparation method of the plant fermentation extract is different, as follows: S1. Remove impurities from green tea, white fungus, rose and licorice, wash them three times with deionized water, dry them until the moisture content is 5%, pulverize them, pass them through a 100-mesh sieve, and mix them to obtain a mixture in which the mass ratio of green tea, white fungus, rose and licorice is 15:8:25:15. S2. Add purified water to the mixture, adjust the solid-liquid ratio to 1:10, stir at 900 rpm until homogeneous, adjust the pH to 6.2, sterilize at 121℃ for 25 min, and cool to 30℃ to obtain plant slurry; inoculate the plant slurry with Aspergillus oryzae, controlling the inoculation amount of Aspergillus oryzae to 4% of the mass of the plant slurry, stir at 800 rpm until homogeneous, and ferment at 30℃ and a ventilation rate of 0.8 L / min for 60 h to obtain the first fermentation product; S3. Add purified water to the first fermentation product, adjust the solid-liquid ratio to 1:7, stir at 900 rpm until homogeneous, sterilize at 115℃ for 18 min, cool to 28℃, inoculate the adjusted system with Lactobacillus plantarum, and ferment at 28℃ for 42 h to obtain the second fermentation product. The inoculation amount of Lactobacillus plantarum is 2.5% of the mass of the adjusted system. S4. The second fermentation product was ultrasonically extracted for 38 minutes at a power of 350W and a temperature of 55℃. The residue was removed by filtration, and the filtrate was collected and concentrated under reduced pressure at a vacuum of 0.09MPa and a temperature of 55℃ until the solid content was 18% to obtain the plant fermentation extract.

[0034] Comparative Example 5 This comparative example is the same as Example 2, except that the preparation of the plant fermentation extract includes the following preparation steps: S1. Remove impurities from green tea, white fungus, rose and licorice, wash them three times with deionized water, dry them until the moisture content is 5%, pulverize them, pass them through a 100-mesh sieve, and mix them to obtain a mixture in which the mass ratio of green tea, white fungus, rose and licorice is 15:8:25:15. S2. Add purified water to the mixture, adjust the solid-liquid ratio to 1:10, stir at 900 rpm until homogeneous, adjust the pH to 6.2, sterilize at 121℃ for 25 min, and cool to 30℃ to obtain plant slurry; inoculate the plant slurry with Aspergillus oryzae, controlling the inoculation amount of Aspergillus oryzae to 4% of the mass of the plant slurry, stir at 800 rpm until homogeneous, and ferment at 30℃ and a ventilation rate of 0.8 L / min for 60 h to obtain the first fermentation product; S3. Add purified water to the first fermentation product, adjust the solid-liquid ratio to 1:7, stir evenly at 900 rpm, sterilize at 115℃ for 18 min, cool to 28℃, inoculate the adjusted system with brewer's yeast, and ferment at 28℃ for 42 h to obtain the second fermentation product. The amount of brewer's yeast inoculated is 2.5% of the mass of the adjusted system. S4. The second fermentation product was ultrasonically extracted for 38 minutes at a power of 350W and a temperature of 55℃. The residue was removed by filtration, and the filtrate was collected and concentrated under reduced pressure at a vacuum of 0.09MPa and a temperature of 55℃ until the solid content was 18% to obtain the plant fermentation extract.

[0035] Performance testing To verify the comprehensive performance of the antioxidant and anti-aging skin repair compositions prepared in Examples 1-3 and Comparative Examples 1-5 of this application, the specific test contents are as follows: Eighty healthy Chinese women aged 30-50 years with sensitive skin, dry and rough facial skin, poor barrier function, loose and inelastic facial skin, and visible fine lines were selected and divided into eight groups. Each group used the antioxidant and anti-aging skin repair compositions prepared in Examples 1-3 and Comparative Examples 1-5, respectively. The stratum corneum moisture content of the test sites was tested, using a before-and-after control method. The stratum corneum moisture content, skin roughness (SEr), and skin elasticity (R2) of the test sites were measured before use, after 14 days of continuous use, and after 28 days of use. Table 1 Performance parameters of the antioxidant and anti-aging skin repair compositions prepared in Examples 1-3 and Comparative Examples 1-5 As shown in Table 1, the antioxidant and anti-aging skin repair composition prepared in this application has outstanding effects in improving skin texture. Relying on the moisturizing and water-locking properties of polyols and the barrier-strengthening effect of ectoine, combined with the hydrating effect of plant fermentation extracts, it maintains the skin's hydration for a long time, avoiding skin problems caused by dryness. The hyaluronic acid peptide, palmitoyl tripeptide-5, hexapeptide-9, palmitoyl tetrapeptide-7, carnosine, and acetyl tetrapeptide-11 in the repair and soothing ingredients have a multi-target synergistic effect, are gentle and suitable for various skin types, and cover all functional dimensions without any repair shortcomings. The repair and soothing ingredients, together with pterostilbene and ergothioneine, can reduce the accumulation of stratum corneum, lighten fine lines, make the skin feel more delicate and smooth, significantly reduce roughness, and effectively improve skin laxity and restore skin firmness. This composition provides long-lasting hydration, smooth skin texture, and firming and elasticity. It has also passed safety tests and has no risk of irritation or allergies. It is suitable for various skin types, including dry, normal, combination, and sensitive skin, to meet the anti-aging and repair needs of different groups.

[0036] This specific embodiment is merely an explanation of this application and is not intended to limit it. After reading this specification, those skilled in the art can make modifications to this embodiment without contributing any inventive step, but such modifications are protected by patent law as long as they fall within the scope of the claims of this application.

Claims

1. An antioxidant, anti-aging skin repair composition characterized in that, By weight percentage, the following components are included: pterostilbene 0.1-0.3%, ergothioneine 0.05-0.5%, repair soothing ingredients 0.15-0.6%, plant fermentation extract 1.0-3.0%, ectoine 0.2-0.5%, composite antioxidant 0.2-1.2%, polyhydric alcohol 0.1-0.3%, surfactant 0.05-0.5%, and the balance is purified water.

2. The antioxidant, anti-aging skin treatment composition of claim 1, wherein, The repair soothing ingredients include hyaluronic acid peptide, palmitoyl tripeptide-5, hexapeptide-9, palmitoyl tetrapeptide-7, carnosine, acetyl tetrapeptide-11.

3. The antioxidant, anti-aging skin treatment composition of claim 2, wherein, The content of each component in the repair soothing ingredients is as follows by weight percentage of the composition: Hyaluronic acid peptide: 0.03-0.12%; Palmitoyl tripeptide-5: 0.02-0.09%; Hexapeptide-9: 0.02-0.07%; Palmitoyl tetrapeptide-7: 0.02-0.08%; Carnosine: 0.04-0.17%; Acetyl tetrapeptide-11: 0.02-0.07%.

4. The antioxidant, anti-aging skin treatment composition of claim 1, wherein, The preparation of the plant fermentation extract includes the following preparation steps: S1, remove impurities from green tea, tremella, rose and licorice respectively, wash and dry to moisture content ≤8%, crush, sieve and mix to obtain a mixture; S2, add purified water to the mixture, stir evenly, adjust the pH to 6.0-6.5, sterilize, and cool to 28-32℃ to obtain a plant slurry; inoculate Aspergillus oryzae in the plant slurry, stir evenly, and ferment at a temperature of 28-32℃ and a ventilation rate of 0.5-1.0 L / min for 48-72 h to obtain a first fermentation product; S3, add purified water to the first fermentation product, stir evenly, sterilize, and cool to 25-30℃; inoculate the adjusted system with a composite bacteria at a temperature of 25-30℃ for 36-48 h to obtain a second fermentation product; S4, ultrasonic extraction, filtration to remove residues, collection of filtrate, and concentration under reduced pressure to obtain a plant fermentation extract.

5. The antioxidant, anti-aging skin treatment composition of claim 4, wherein, The inoculation amount of Aspergillus oryzae in step S2 is 3-5% of the mass of the plant slurry.

6. The antioxidant, anti-aging skin treatment composition of claim 4, wherein, The composite bacteria in step S3 are composed of Lactobacillus plantarum and Saccharomyces cerevisiae with effective viable counts of 8-12:3-5.

7. The antioxidant, anti-aging skin treatment composition of claim 4, wherein, The inoculation amount of the composite bacteria in step S3 is 1.5-3.2% of the mass of the adjusted system.

8. The antioxidant, anti-aging skin treatment composition of claim 1, wherein, The composite antioxidant is composed of oryzanol, vitamin E and phytosterol in a mass ratio of 10-18:5-8:10-12.

9. The antioxidant, anti-aging skin treatment composition of claim 1, wherein, The polyhydric alcohol is one or more of propylene glycol, 1,3-propanediol, glycerol, diglycerol, butanediol, pentanediol and hexanediol; and the surfactant is lecithin and / or PPG-13-decyltetradecinol polyether-24.

10. A process for the preparation of an antioxidant, anti-aging skin care composition as claimed in any one of claims 1 to 9, characterized in that, The preparation includes the following steps: Step 1, add polyol into purified water, heat to 65-70℃, stir for 10-15 min, add ectoine, continue to stir for 8-10 min, obtain water phase; add surfactant into composite antioxidant, stir for 10-15 min at temperature of 40-45℃, obtain oil phase; pump oil phase into water phase, control oil phase pumping speed to be 5-8 mL / min, maintain temperature at 60-65℃, stir for 20-25 min at stirring speed of 1500-2000 rpm, obtain emulsion; Step 2, homogenize the emulsion, add repair soothing ingredients, stir for 15-20 min, continue to cool to 38-40℃, add pterostilbene, ergothioneine and plant fermentation extract, stir for 10-15 min, cool to below 30℃, adjust pH to 5.5-6.5, stir for 5-8 min, filter, obtain antioxidant, anti-aging skin repair composition.

Images