Synergistic antioxidant composition

Abstract

The present invention provides a synergistic antioxidant composition comprising: 1 to 5 wt% of glycine; 1 to 5 wt% of arginine; and water, wherein the pH value of the composition is 8 to 10. The weight ratio of glycine to arginine in the composition is (2: 1)-(5: 1). The invention also relates to a preparation method of the synergistic anti-oxidation composition and a skin external preparation containing the synergistic anti-oxidation composition.

Description

Technical Field

[0001] This invention relates to the fields of fine chemicals and cosmetics, specifically to a synergistic antioxidant composition, its preparation method, and its application. Background Technology

[0002] The skin is one of the largest organs in the human body and serves as the first natural barrier protecting the body from external aggressors. Normal bodily functions inevitably produce free radicals, but the body's own antioxidant defense system effectively eliminates them. Therefore, a healthy body maintains a dynamic balance between free radical production and elimination, preventing damage. It is well known that excessive exposure to solar radiation, smoking, and environmental pollution can trigger the production of excessive free radicals. These excess free radicals damage the skin's physiological structure, leading to dullness, lack of radiance, sagging, age spots, wrinkles, and other signs of aging.

[0003] Antioxidants are an important component of cosmetics, possessing the ability to inhibit cell damage. Topical application of antioxidants to the skin is crucial for minimizing skin aging and better protecting the skin from harmful free radicals. For the product itself, antioxidants can prevent and delay the discoloration reactions of other active ingredients in cosmetics and can prevent the oxidative rancidity of the oils used. Currently, commonly used antioxidants in cosmetics are divided into two categories: one is enzymatic antioxidants, mainly including superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-PX), and the thioredoxin (Trx) system; the other is non-enzymatic antioxidants, mainly including vitamin C, vitamin E, coenzyme Q10, glutathione, alpha-lipoic acid, carotenoids, and natural plant polyphenols.

[0004] Existing work focuses more on finding natural or highly effective antioxidants. For example, Chinese patent CN107106464B provides an antioxidant containing enzymatically synthesized glycogen (ESG) or its α-amylase digest (RG); Chinese patent CN100333709C provides an antioxidant containing acerola cherry seeds as an active ingredient that has high antioxidant activity and can be used in cosmetics by treating acerola cherry seeds, which are treated as waste.

[0005] Arginine (L-Arginine) is a semi-essential amino acid widely found in various foods, such as meat, fish, dairy products, and nuts. It plays a number of important physiological functions and provides numerous health benefits in the human body, including promoting cardiovascular health, enhancing immune function, improving athletic performance, promoting wound healing, and possessing antioxidant properties. Arginine exhibits significant antioxidant activity, mitigating oxidative stress-induced cell damage by activating the NRF2 signaling pathway and other antioxidant enzyme systems. Furthermore, arginine slows down cellular aging by inhibiting the activity of the autophagy enzyme EP300. Arginine can also improve mitochondrial efficiency and promote energy metabolism, thereby alleviating fatigue-related discomfort.

[0006] Glycine, the simplest amino acid in nature with the chemical formula C2H5NO2, possesses numerous important physiological functions and health benefits. While a non-essential amino acid, glycine, as one of the basic building blocks of proteins, participates in protein synthesis in the body, providing raw materials for cell division and growth. Furthermore, glycine can convert α-keto acids into non-essential amino acids via mesolysis, meeting the body's needs. Glycine is a key component of glutathione (GSH), a powerful antioxidant that scavenge free radicals and protects cells from oxidative damage. In addition, glycine metabolism in the liver promotes glycogen storage or its conversion into other nitrogenous compounds, thereby improving liver function.

[0007] Glycine can enhance the body's immunity by promoting the energy metabolism and function of immune cells, thereby strengthening the body's disease resistance. In addition, it has anti-inflammatory effects, inhibiting inflammatory responses. Glycine has antispasmodic properties, relieving tension in gastrointestinal smooth muscle and reducing abdominal pain and muscle spasms. It can also alleviate fatigue by regulating neurotransmitter balance. Glycine plays a significant role in cardiovascular health. It can reduce the risk of acute myocardial infarction, inhibit chronic rejection of aortic dissection, and improve angiogenesis through its antioxidant effects. Furthermore, glycine also has antihypertensive effects.

[0008] Glycine has anti-aging properties, slowing down cell aging and reducing oxidative stress. Furthermore, it is used to treat mental illnesses such as schizophrenia and depression by improving cognitive function through regulating neurotransmitter balance. Glycine can also inhibit the release of inflammatory factors and reduce immune responses.

[0009] While the antioxidant properties of raw materials for other applications, such as skin protectants, have received little attention, this application unexpectedly discovered that arginine and glycine, as skin protectants, possess free radical scavenging capabilities against dibenzopicrin (DPPH) free radicals. DPPH is a very stable nitrogen-centered free radical. If the test substance can scavenge it, it suggests that the test substance has the effective ability to reduce the concentration of free radicals such as hydroxyl radicals, alkyl radicals, or peroxide radicals, thereby interrupting the lipid peroxidation chain reaction. Therefore, this application finds that the combination of arginine and glycine not only has synergistic antioxidant properties but also skin-protective effects, providing better protection for the skin. Summary of the Invention

[0010] On one hand, the present invention provides a synergistic antioxidant composition comprising: 1-5% by weight of glycine; 1-5% by weight of arginine; and water, wherein the pH of the composition of the invention is 8-10.

[0011] In a preferred embodiment, the weight ratio of glycine to arginine in the composition of the present invention is 2:1 to 5:1.

[0012] In a preferred embodiment, the pH value of the composition of the invention is 8.56-9.62.

[0013] In a preferred embodiment, the pH value of the composition of the present invention is 8.56-8.87.

[0014] In a preferred embodiment, the composition of the present invention contains 4-5% by weight of glycine.

[0015] In a preferred embodiment, the composition of the present invention contains 1-2% by weight of arginine.

[0016] In a preferred embodiment, the composition of the present invention has a DPPH scavenging rate of ≥33.24%.

[0017] On the other hand, the present invention also relates to a method for preparing the composition, comprising the following steps: weighing glycine and arginine, adding deionized water to make up to 100 parts by mass, mixing evenly, and allowing to stand to obtain the composition of the present invention.

[0018] In another aspect, the present invention also relates to a topical skin preparation comprising the aforementioned synergistic antioxidant composition, wherein the antioxidant composition is present in the topical skin preparation at a concentration of 0.3-10% by weight.

[0019] In a preferred embodiment, the antioxidant composition is present in the topical skin agent at a concentration of 0.3-6% by weight. Detailed Implementation

[0020] The present invention will be further illustrated below with reference to specific embodiments. However, these embodiments do not constitute any limitation on the present invention and are only used to illustrate the present invention. Any limited modifications made within the scope of the claims of the present invention are still within the scope of the claims of the present invention.

[0021] The purpose of this invention is to provide a synergistic antioxidant composition. The synergistic antioxidant composition prepared according to a specific mass ratio and preparation method can not only obtain a composition with enhanced antioxidant efficacy, but also have skin protection effects.

[0022] To provide a more concise description, some of the quantitative expressions given herein are not modified by the term "approximately". It should be understood that, whether or not the term "approximately" is explicitly used, each quantity given herein is intended to refer to an actual given value, and also to an approximation of such given values ​​that can be reasonably inferred by one of ordinary skill in the art, including approximations of such given values ​​caused by experimental and / or measurement conditions.

[0023] Arginine Arginine possesses significant antioxidant properties, mitigating oxidative stress-induced cell damage by activating the NRF2 signaling pathway and other antioxidant enzyme systems. Furthermore, arginine delays cellular senescence by inhibiting the activity of the autophagy enzyme EP300. However, arginine production is costly, especially given rising raw material prices; therefore, more cost-effective antioxidant additives are needed.

[0024] In some embodiments, the compositions of the present invention contain 1-5% by weight of arginine. Preferably, the compositions of the present invention contain 1-2% by weight of arginine.

[0025] In a specific implementation, the present invention uses L-arginine, a product purchased from Shanghai Xiehe Amino Acid Co., Ltd.

[0026] glycine Glycine is a potent antioxidant that scavenge free radicals, protects cells from oxidative damage, and inhibits inflammatory responses, thereby reducing the risk of various chronic diseases. As a non-essential amino acid, glycine is generally well-tolerated with few adverse reactions. It has wide applications in food, pharmaceuticals, and cosmetics, such as as a sweetener, buffer, and antioxidant. Although glycine is already used as an antioxidant in cosmetics, the cosmetic industry still needs more cost-effective antioxidant additives.

[0027] In some embodiments, the compositions of the present invention contain 1-5% by weight of glycine. Preferably, the compositions of the present invention contain 4-5% by weight of glycine.

[0028] In a specific implementation, the present invention uses glycine, a product purchased from Hebei Huayang Biotechnology Co., Ltd.

[0029] Synergistic Antioxidant Composition This invention provides a synergistic antioxidant composition.

[0030] The synergistic antioxidant composition of the present invention comprises: 1-5% by weight of glycine; 1-5% by weight of arginine; and water.

[0031] In some embodiments, the weight ratio of glycine to arginine is 2:1 to 5:1.

[0032] In some embodiments, the pH of the composition is 8-10, preferably 8.56-9.62.

[0033] In some embodiments, the pH value of the composition is 8.56-8.87.

[0034] In some embodiments, the composition is in the form of an aqueous solution.

[0035] Preparation method of the composition This invention also relates to a method for preparing a synergistic antioxidant composition. Specifically, the method includes the following steps: weighing glycine and arginine, adding deionized water to a final volume of 100 parts by weight, mixing thoroughly, and allowing to stand to obtain the composition of this invention.

[0036] Topical skin agents In some specific embodiments, the topical skin agent of the present invention is a combination of glycine and arginine, which can be used as an efficacy additive in the preparation of the topical skin agent. The topical skin agent is preferably a cosmetic composition, including but not limited to the preparation of products in dosage forms such as creams, lotions, and sprays. The weight ratio of the composition containing glycine and arginine in the topical skin agent is 2:1 to 5:1, with a preferred weight ratio of 5:1.

[0037] The present invention will be further illustrated below with reference to specific embodiments. It is important to note that these embodiments are for illustrative purposes only and should not be construed as limiting the scope of protection of the invention. Those skilled in the art can make non-essential improvements and adjustments based on the above description of the invention. Test methods in the following embodiments that do not specify specific conditions are generally performed under conventional conditions or as recommended by the manufacturer. Unless otherwise stated, all percentages and parts are by weight.

[0038] Example The experimental materials used in the embodiments of the present invention are as follows: Glycine, trade name: glycine, purchased from Hebei Huayang Biotechnology Co., Ltd.; Arginine, trade name: L-arginine, purchased from Shanghai Xiehe Amino Acid Co., Ltd.; The water used in the experiment was deionized water prepared in the laboratory. Anhydrous ethanol was purchased from Sinopharm Chemical Reagent Co., Ltd. DPPH solution, purchased from SIGMA; The ultraviolet-visible spectrometer, model CARY 100 Conc, with a test wavelength of 517 nm, was purchased from VARIAN. The pH meter, SevenExcellence S500-Basic, was purchased from Mettler.

[0039] Example 1: Preparation of an antioxidant composition Weigh glycine and arginine sequentially according to Table 1, and add deionized water to a final volume of 100 parts by weight. Allow the mixture to stand at room temperature and normal pressure, protected from light, until the sample is completely dissolved before proceeding with the experiment. Table 1: Content of each component in the antioxidant compositions prepared in Examples 1-15.

[0040] Examples 1-10 are for individual raw materials, and Examples 11-15 are for formulations of glycine and arginine obtained through orthogonal experiments.

[0041] Test Example 1: Visual Observation and pH Measurement The pH values ​​of the compositions prepared in Examples 1-15 were measured, and their appearance was observed at room temperature and pressure. Table 2 shows the pH values ​​and appearance of the compositions prepared in Examples 1-10.

[0042] Table 3. pH values ​​and appearance of the compositions prepared in Examples 11-15

[0043] Glycine has a weakly acidic pH, while arginine solution has a higher pH. In the examples of their combination, the pH is between 8 and 10. In addition to being used as a skin protectant, it can also be used to adjust the pH of the system.

[0044] Test Example 2: DPPH Free Radical Scavenging Rate Determination The DPPH free radical scavenging rate of the compositions prepared in Examples 1-15 was determined.

[0045] DPPH solution: Accurately weigh 20 mg of DPPH, dissolve it in anhydrous ethanol, and dilute to a final volume of 250 mL in a volumetric flask. The DPPH concentration is then 2 × 10⁻⁶. -4mol / L. Take 100 μL of the test solution and an equal volume of 2 × 10⁻⁶ mol / L. -4 Add mol / L DPPH solution to the wells of the same 96-well plate, mix thoroughly, and incubate the plate at room temperature for 30 min in the dark (the incubation time should be fixed to ensure the free radical reaction reaches equilibrium). Use a microplate reader to measure the absorbance of the blank group, sample group, and background group sequentially at 517 nm. After 30 min, use anhydrous ethanol as a reference to measure the absorbance (Ai). 2 × 10⁻⁶ -4 The absorbance of the mixture of mol / L DPPH solution and an equal volume of anhydrous ethanol is Ac; the absorbance of the test solution and an equal volume of anhydrous ethanol mixture is Aj.

[0046] Calculate the clearance rate using the following formula: Clearance rate = [1 - (Ai - Aj) / Ac] × 100% Ac: Absorbance of DPPH solution without test solution; absorbance of blank group (absorbance of DPPH and solvent only, representing the initial free radical concentration).

[0047] Ai: Absorbance of DPPH solution when the test solution is added; Absorbance of sample group (absorbance of residual free radicals after the sample reacts with DPPH).

[0048] Aj: Absorbance of the test liquid at the measurement wavelength, background absorbance of the sample (absorbance of the sample itself at 517nm, eliminating background interference).

[0049] The DPPH scavenging rates of Examples 1-15 were determined respectively. Table 4, DPPH removal rates in Examples 1-10

[0050] Examples 1-10 show that both glycine and arginine solutions exhibit a certain DPPH scavenging ability, with the arginine solution prepared in Examples 6-10 being slightly better than the glycine solution prepared in Examples 1-5.

[0051] Table 5, DPPH removal rate in Examples 11-15

[0052] Based on the results of Examples 11-15, Example 15 exhibited the highest DPPH radical scavenging rate, exceeding that of the glycine solution in Example 5 and the arginine solution in Example 6, which showed the highest rates among individual raw materials. This indicates that the combination of the two can enhance antioxidant capacity. The DPPH radical scavenging rate of Example 14 was also superior to that of the glycine solution in Example 4 and the arginine solution in Example 7. However, the DPPH radical scavenging rates of Examples 11-13 did not show any superiority over either the glycine solution or the arginine solution alone. This suggests that glycine and arginine solutions only exhibit a synergistic effect in DPPH radical scavenging when combined in a specific way.

[0053] Based on the examples and test cases, it is believed that when the mass ratio of glycine to arginine is 2:1 to 5:1, the solution containing glycine and arginine exhibits a significant synergistic DPPH free radical scavenging ability, that is, the combination of the two can enhance the antioxidant capacity, thus obtaining a composition with antioxidant capacity.

[0054] Application examples The composition containing glycine and arginine described in this invention can be used as an efficacy additive in the preparation of topical skin agents. The topical skin agent is preferably a cosmetic composition, including but not limited to the preparation of products in dosage forms such as creams, lotions, and sprays. The weight ratio of the composition containing glycine and arginine in the topical skin agent is 2:1 to 5:1, with a preferred weight ratio of 5:1, and the total weight percentage does not exceed 10%.

[0055] The following are specific examples of the application of compositions containing glycine and arginine in topical skin preparations, including creams, lotions, and sprays, as well as the formulations and preparation methods of these dosage forms. Specific applications are as follows: Application Example 1: Preparation of Creams and Ointments

[0056] Process: Heat phase A to 80℃, disperse it evenly under homogenization, and add phase B heated to 80℃. After adding, homogenize for 5 minutes, stir and cool to 55℃, add phase C, stir and cool to 50℃, add phase D, homogenize for 3 minutes, stir and cool to 35℃ to obtain the final product.

[0057] Application Example 2: Emulsion Preparation

[0058] Process: Heat phase A to 80℃, disperse it evenly under homogenization, and add phase B heated to 80℃. After adding, homogenize for 5 minutes, stir and cool to 55℃, add phase C, stir and cool to 50℃, add phase D, homogenize for 3 minutes, stir and cool to 35℃ to obtain the final product.

[0059] Application Example 3: Preparation of Spray

[0060] Process: Heat phase A to 80℃, disperse it evenly under homogenization, and add phase B heated to 80℃. After adding, homogenize for 5 minutes, stir and cool to 55℃, add phase C, stir and cool to 50℃, add phase D, homogenize for 3 minutes, stir and cool to 35℃ to obtain the final product.

Claims

1. A synergistic antioxidant composition comprising: 1-5% by weight glycine; 1-5% by weight arginine; and water, wherein the composition has a pH of 8-10.

2. The composition according to claim 1, characterized in that, The weight ratio of glycine to arginine in the composition is 2:1 to 5:

1.

3. The composition according to claim 1, characterized in that, The pH value of the composition is 8.56-9.

62.

4. The composition according to claim 3, characterized in that, The pH value of the composition is 8.56-8.

87.

5. The composition according to claim 1, characterized in that, The composition contains 4-5% by weight of glycine.

6. The composition according to claim 1, characterized in that, The composition contains 1-2% by weight of arginine.

7. The composition according to claim 1, characterized in that, The composition has a DPPH scavenging rate of ≥33.24%.

8. A method for preparing the composition according to any one of claims 1-7, comprising the following steps: weighing glycine and arginine, adding deionized water to make up to 100 parts by mass, mixing evenly, and allowing to stand to obtain the composition of the present invention.

9. A topical skin preparation comprising the synergistic antioxidant composition according to any one of claims 1-7, characterized in that, The antioxidant composition is present in the topical skin preparation at a concentration of 0.3-10% by weight.

10. The topical skin agent as described in claim 9, characterized in that, The antioxidant composition is present in the topical skin preparation at a concentration of 0.3-6% by weight.

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