Method for extracting glycolipid compound from soybean phospholipid

Abstract

The invention discloses a method for extracting glycolipid compounds from soybean phospholipids, which comprises the following steps: step 1, adding soybean phospholipids particles into soybean oil, and stirring to fully dissolve the soybean phospholipids particles; step 2, adding water and phospholipase into the dissolved soybean phospholipid oil solution, stirring to carry out an enzymolysis reaction, and heating to inactivate phospholipase after the reaction is completed, so as to obtain a soybean phospholipid enzymolysis product; step 3, distilling the soybean phospholipid enzymolysis product to obtain a concentrated solution; step 4, adding acetone into the concentrated solution to fully dissolve the concentrated solution, centrifuging, collecting the precipitate, and drying; and 5, adding water into the precipitate obtained in the step 4, fully dissolving the precipitate, centrifuging, collecting the precipitate, and drying to obtain the glycolipid compound. The method provided by the invention has the characteristics of high purity of the prepared product, mild action conditions and short time consumption, and can realize batch production of glycolipid.

Description

TECHNICAL FIELD

[0001] The application belongs to the technical field of glycolipid preparation method, and relates to a method for extracting glycolipid compounds from soybean phospholipids. BACKGROUND

[0002] Glycolipids are compounds containing one or more monosaccharide moieties combined with lipid moieties through glycosidic bonds. Glycolipids in soybean phospholipids include four categories: sterol glucosides (SG), acylated sterol glucosides (ASG), cerebrosides (CE), and digalactosyl diacylglycerides (DGDG).

[0003] Glycolipids have good surface activity. In food processing, glycolipids can be used as a natural emulsifying stabilizer to enhance food texture, taste and nutritional value. At the same time, glycolipids also have good biological activity. In the medical field, glycolipids can reduce cholesterol, regulate blood sugar, improve immunity, sterilize and resist cancer. Glycolipids are widely used in cosmetics, skin care products, environmental protection fields and petroleum industry fields.

[0004] Currently, glycolipids are mainly prepared by artificial synthesis, fermentation and column chromatography extraction methods. However, these methods have problems such as high cost, serious pollution, harsh conditions and low product stability. SUMMARY

[0005] The purpose of the present application is to provide a method for extracting glycolipid compounds from soybean phospholipids, which has the characteristics of high purity of the obtained product, mild action conditions, short time consumption, and can realize batch production of glycolipids.

[0006] The technical solution adopted by the present application is a method for extracting glycolipid compounds from soybean phospholipids, which is implemented according to the following steps: Step 1, adding soybean phospholipid particles into soybean oil and stirring to make the soybean phospholipid particles fully dissolved; Step 2, adding water and phospholipase into the dissolved soybean phospholipid oil solution, stirring to make it undergo enzymatic reaction, heating to inactivate the phospholipase after the reaction is completed, and obtaining a soybean phospholipase hydrolysate; Step 3, distilling the soybean phospholipase hydrolysate to obtain a concentrated solution; Step 4, adding acetone into the concentrated solution to make the concentrated solution fully dissolved, centrifuging, collecting the precipitate and drying; Step 5, adding water into the precipitate obtained in step 4 to make the precipitate fully dissolved, centrifuging, collecting the precipitate and drying to obtain glycolipid compounds.

[0007] The application also has the characteristics that: In step 1, the mass ratio of the soybean phospholipid particles to the soybean oil is 1:5-10.

[0008] In step 1, the stirring temperature is 75-85℃, the stirring speed is 30-60rpm, and the stirring time is 1-4h.

[0009] In step 2, the mass ratio of the water to the soybean phospholipid particles is 15-25:1, the phospholipase is phospholipase A1, and the enzyme concentration is 0.2-1.4% of the total mass of the reactants.

[0010] In step 2, the stirring temperature is 30-55℃, the stirring speed is 30-60rpm, and the enzymatic reaction time is 1-9h; after the reaction is completed, the temperature is heated to 85-95℃ for 5-20min to inactivate the phospholipase.

[0011] Step 3 is specifically: The soybean phospholipid enzymatic product is subjected to vacuum rotary distillation for concentration, the distillation temperature is 70-95℃, the stirring speed is 50-150rpm, the pressure is 0.05-0.1Mpa, and the distillation time is 3-10h, and the concentrated liquid is obtained after distillation.

[0012] In step 4, the addition amount of acetone is: 20-30mL of acetone is added per 1g of soybean phospholipid particles.

[0013] In step 4, the steps of adding acetone, centrifuging and collecting the precipitate are performed twice, wherein the centrifugal speed is 8000-10000rpm, and the centrifugal time is 5-20min; the drying is hot air drying, the temperature is 35-50℃, and the time is 0.5-2h.

[0014] In step 5, the mass ratio of the added water to the soybean phospholipid particles is 30-40:1.

[0015] In step 5, the steps of adding water, centrifuging and collecting the precipitate are performed twice, wherein the centrifugal speed is 8000-10000rpm, the centrifugal time is 20-40min; the drying is hot air drying, the temperature is 40-60℃, and the time is 4-10h.

[0016] The application has the beneficial effects that: (1) The method of the application uses an enzymatic method to extract natural glycolipids from soybean phospholipids, which is a green and environmentally friendly glycolipid extraction method, and is expected to become a method for large-scale production of natural glycolipids; (2) At present, most of the research on natural soybean phospholipids is focused on phospholipid components, and the method of the application extracts glycolipids from soybean phospholipids as raw materials, providing a new way for the deep processing of soybean phospholipids; (3) The method of the application uses acetone / water to extract glycolipids from phospholipid hydrolysate, and the extracted glycolipids have the characteristics of high purity. Attached Figure Description

[0017] Figure 1 The liquid chromatogram of the glycolipid compound obtained in Example 1 of this invention; Figure 2 The liquid chromatogram of the glycolipid compound obtained in Example 2 of this invention; Figure 3 The liquid chromatogram of the glycolipid compound obtained in Example 3 of this invention; Figure 4 The liquid chromatogram of the glycolipid compound obtained in Comparative Example 1 of this invention; Figure 5 This is a liquid chromatogram of the glycolipid compound obtained in Comparative Example 2 of the present invention; Figure 6 This is a liquid chromatogram of the glycolipid compound obtained in Comparative Example 3 of the present invention. Detailed Implementation

[0018] The present invention will now be described in detail with reference to the accompanying drawings and specific embodiments.

[0019] The method for extracting glycolipid compounds from soybean phospholipids according to the present invention is implemented according to the following steps: Step 1: Take soybean lecithin granules and add them to soybean oil. The mass ratio of soybean lecithin granules to soybean oil is 1:5-10. Place both in a constant temperature magnetic stirrer and stir at a temperature of 75-85℃, a speed of 30-60 rpm, and a time of 1-4 hours to fully dissolve the soybean lecithin granules. Step 2: Add water and phospholipase to the dissolved soybean lecithin oil solution. The mass ratio of water to soybean lecithin particles is 15-25:1. The phospholipase is phospholipase A1, and the enzyme concentration is 0.2-1.4% of the total system (which includes soybean lecithin, soybean oil, water, and phospholipase). Stir to allow the enzymatic hydrolysis reaction to occur. The stirring temperature is 30-55℃, the stirring speed is 30-60 rpm, and the enzymatic hydrolysis reaction time is 1-9 hours. After the reaction is completed, heat to 85-95℃ and maintain for 5-20 minutes to inactivate the phospholipase, obtaining the soybean lecithin enzymatic hydrolysis product. Step 3: Concentrate the soybean phospholipid enzymatic hydrolysate by vacuum rotary distillation. The distillation temperature is 70-95℃, the rotation speed is 50-150 rpm, the pressure is 0.05-0.1 MPa, and the distillation time is 3-10 h. The concentrated liquid is obtained after distillation. Step 4, add acetone to the concentrated solution to fully dissolve, the volume of acetone added is: 20-30 mL of acetone per 1 g of soybean phospholipid particles; collect the precipitate after centrifugation, add acetone again to dissolve, collect the precipitate after centrifugation and dry, wherein the centrifugal speed is 8000-10000 rpm, the centrifugal time is 5-20 min, the drying is hot air drying, the temperature is 35-50℃, and the time is 0.5-2 h; Step 5, add water to the precipitate to fully dissolve, wherein the mass ratio of water to soybean phospholipid particles is 30-40:1, collect the precipitate after centrifugation, add water again to dissolve, collect the precipitate after centrifugation and dry to obtain a glycolipid compound. The centrifugal speed is 8000-10000 rpm, the centrifugal time is 20-40 min, the drying is hot air drying, the temperature is 40-60℃, and the time is 4-10 h.

[0020] Glycolipids are compounds containing one or more sugar moieties combined with lipid moieties through glycosidic bonds. Due to the presence of hydroxyl groups in glycolipids, they are often removed from oil together with phospholipids and other colloidal substances during oil degumming. Soybean phospholipids contain about 6-10% glycolipids, which are significantly higher than the content of glycolipids in other plants, making them an ideal raw material for recovering glycolipids. Phospholipase A1 (PLA1) is an enzyme that specifically hydrolyzes the Sn-1 acyl group of phospholipids, and can obtain Sn-2 acyl lysophosphatidylethanolamine and lysophosphatidylcholine. In the method for extracting glycolipid compounds from soybean phospholipids, steps 4 and 5 remove acetone-soluble and water-soluble phospholipid hydrolysates such as free fatty acids and lysophospholipids.

[0021] Example 1: Step 1, take 2 g of soybean phospholipid particles and add them to 20 g of soybean oil, place them in a constant-temperature magnetic stirrer, dissolve at a temperature of 80℃, rotate at a speed of 40 rpm, and stir for 2 h to fully dissolve; Step 2, add 40 g of water and 1.0% by mass of phospholipase A1 to the dissolved soybean phospholipid oil solution, place it in a constant-temperature magnetic stirrer, enzymatic hydrolysis temperature is 50℃, rotation speed is 40 rpm, enzymatic hydrolysis time is 3 h, after the reaction is completed, it is placed in heating at 90℃ for 10 min to inactivate the phospholipase, and the soybean phospholipid hydrolysate is obtained; Step 3, perform vacuum rotary evaporation on the soybean phospholipid hydrolysate, temperature is 90℃, rotation speed is 150 rpm, pressure is 0.1 Mpa, evaporation time is 6 h, and the concentrated solution is obtained; Step 4, add 50 mL of acetone to the concentrated solution to fully dissolve, centrifuge at a speed of 10000 rpm for 10 min, collect the precipitate after centrifugation, add 50 mL of acetone to the precipitate to fully dissolve, centrifuge at a speed of 10000 rpm for 10 min, collect the precipitate after centrifugation, and dry at 40℃ for 0.5 h; Step 5, 70 mL water was added to the precipitate to dissolve thoroughly, centrifuged at 10000 rpm for 30 min, the precipitate was collected after centrifugation, 70 mL water was added to the precipitate to dissolve thoroughly, centrifuged at 10000 rpm for 30 min, the precipitate was collected after centrifugation, and hot air drying at 60℃ for 4h to obtain the glycolipid compound.

[0022] The glycolipid compound obtained in this example was detected by HPLC-ELSD, and the method was as follows: 1. Sample: The glycolipid compound sample was dissolved with chloroform / methanol (2:1, v / v), and filtered with a 0.45 μm filter; 2. Instrument: Agilent 1260 high performance liquid chromatograph, Agilent 1260 Infinity III evaporative light scattering detector; 3. Chromatographic separation: C18 (4.6 250mm, 5μm) column, 1 mL / min flow rate, column temperature 30℃, mobile phase A: chloroform, mobile phase B: methanol / water (95:5, v / v), separation gradient: 0-15min, 99%A-75%A, 15-20min, 75%A-10%A, 20-25min, 10%A, 25-30min, 10%A-99%A, 30-40min, 99%A; 4. ELSD detection: nitrogen flow 3.5 L / min, drift tube temperature 80℃.

[0023] The liquid chromatogram of the glycolipid compound is shown in Figure 1 After detection and calculation, the purity of ASG was 1.56%, the purity of SG was 42.33%, and the purity of CE was 5.03%; the recovery rate of ASG was 1.94%, the recovery rate of SG was 52.84%, and the recovery rate of CE was 6.26%; the purity of glycolipid compound was 48.92%, the recovery rate of glycolipid compound was 61.05%, and the yield of glycolipid compound was 13.86%.

[0024] Example 2: Step 1, the same as step 1 of example 1; Step 2, 40 g of water and 1.0% of phospholipase A1 by mass were added to the dissolved soybean phospholipid oil solution, and placed in a constant temperature magnetic stirrer, the enzymolysis temperature was 50℃, the rotation speed was 50 rpm, the enzymolysis time was 1h, after the reaction was completed, it was heated at 90℃ for 15 min to inactivate the phospholipase, and the soybean phospholipid enzymolysis product was obtained; Step 3, the soybean phospholipid enzymolysis product was subjected to vacuum rotary evaporation, the temperature was 90℃, the rotation speed was 150 rpm, the pressure was 0.08 Mpa, and the distillation time was 10h, and the concentrated solution was obtained; Step 4, the same as step 4 of example 1; Step 5, same as step 5 of example 1.

[0025] The sugar lipid compound obtained in this example was detected by HPLC-ELSD, and the results are shown in Table 1. Figure 2 As shown in Table 1, after detection and calculation, the purity of ASG was 1.89%, the purity of SG was 43.15%, and the purity of CE was 5.56%; the recovery rate of ASG was 2.11%, the recovery rate of SG was 48.07%, and the recovery rate of CE was 6.19%; the purity of sugar lipid compound was 50.60%, the recovery rate of sugar lipid compound was 56.36%, and the yield of sugar lipid compound was 12.37%.

[0026] Example 3: Step 1, same as step 1 of example 1; Step 2, 40g of water and 1.0% of phospholipase A1 by mass were added to the soybean phospholipid oil solution after dissolution, and placed in a constant temperature magnetic stirrer, the enzymolysis temperature was 50℃, the rotation speed was 60rpm, the enzymolysis time was 5h, after the reaction was completed, it was placed in 90℃ heating for 10min to inactivate the phospholipase, and the soybean phospholipase hydrolysate was obtained; Step 3, the soybean phospholipase hydrolysate was subjected to vacuum rotary evaporation, the temperature was 95℃, the rotation speed was 100rpm, the pressure was 0.08Mpa, and the evaporation time was 8h, and the concentrated solution was obtained; Step 4, same as step 4 of example 1; Step 5, same as step 5 of example 1.

[0027] The sugar lipid compound obtained in this example was detected by HPLC-ELSD, and the results are shown in Table 1. Figure 3 As shown in Table 1, after detection and calculation, the purity of ASG was 1.89%, the purity of SG was 43.15%, and the purity of CE was 5.56%; the recovery rate of ASG was 2.11%, the recovery rate of SG was 48.07%, and the recovery rate of CE was 6.19%; the purity of sugar lipid compound was 50.60%, the recovery rate of sugar lipid compound was 56.36%, and the yield of sugar lipid compound was 12.37%.

[0028] Example 4: Step 1, 2g of soybean phospholipid particles were added to 10g of soybean oil, and placed in a constant temperature magnetic stirrer, the dissolution temperature was 75℃, the rotation speed was 60rpm, and the stirring time was 2h to make it fully dissolved; Step 2, 30g of water and 1.4% of phospholipase A1 by mass were added to the soybean phospholipid oil solution after dissolution, and placed in a constant temperature magnetic stirrer, the enzymolysis temperature was 30℃, the rotation speed was 40rpm, the enzymolysis time was 7h, after the reaction was completed, it was placed in 85℃ heating for 20min to inactivate the phospholipase, and the soybean phospholipase hydrolysate was obtained; Step 3, the soybean phospholipase hydrolysate was subjected to reduced pressure rotary evaporation, temperature 70℃, rotation speed 150rpm, pressure 0.05Mpa, distillation time 6h, to obtain a concentrated solution; Step 4, 40mL of acetone was added to the concentrated solution to fully dissolve, centrifuged at a rotation speed of 9000rpm for 10min, and the precipitate was collected after centrifugation. Then 40mL of acetone was added to the precipitate to fully dissolve, centrifuged at a rotation speed of 9000rpm for 10min, and the precipitate was collected after centrifugation. The precipitate was hot air dried at 35℃ for 2h; Step 5, 80mL of water was added to the precipitate to fully dissolve, centrifuged at a rotation speed of 10000rpm for 30min, and the precipitate was collected after centrifugation. Then 80mL of water was added to the precipitate to fully dissolve, centrifuged at a rotation speed of 10000rpm for 30min, and the precipitate was collected after centrifugation. The precipitate was hot air dried at 40℃ for 10h, to obtain a glycolipid compound.

[0029] Example 5: Step 1, 2g of soybean phospholipid particles were added to 15g of soybean oil, and placed in a constant temperature magnetic stirrer. The dissolution temperature was 85℃, the rotation speed was 30rpm, and the stirring time was 4h to fully dissolve; Step 2, 50g of water and 0.5% of phospholipase A1 by mass percentage were added to the dissolved soybean phospholipid oil solution, and placed in a constant temperature magnetic stirrer. The enzymolysis temperature was 55℃, the rotation speed was 30rpm, and the enzymolysis time was 8h. After the reaction was completed, it was heated at 95℃ for 5min to inactivate the phospholipase, to obtain a soybean phospholipase hydrolysate; Step 3, the soybean phospholipase hydrolysate was subjected to reduced pressure rotary evaporation, temperature 90℃, rotation speed 50rpm, pressure 0.05Mpa, distillation time 6h, to obtain a concentrated solution; Step 4, 60mL of acetone was added to the concentrated solution to fully dissolve, centrifuged at a rotation speed of 8000rpm for 20min, and the precipitate was collected after centrifugation. Then 60mL of acetone was added to the precipitate to fully dissolve, centrifuged at a rotation speed of 8000rpm for 20min, and the precipitate was collected after centrifugation. The precipitate was hot air dried at 50℃ for 0.5h; Step 5, 60mL of water was added to the precipitate to fully dissolve, centrifuged at a rotation speed of 8000rpm for 40min, and the precipitate was collected after centrifugation. Then 60mL of water was added to the precipitate to fully dissolve, centrifuged at a rotation speed of 8000rpm for 40min, and the precipitate was collected after centrifugation. The precipitate was hot air dried at 50℃ for 8h, to obtain a glycolipid compound.

[0030] Example 6: Step 1, 2g of soybean phospholipid particles were added to 20g of soybean oil, and placed in a constant temperature magnetic stirrer. The dissolution temperature was 75℃, the rotation speed was 60rpm, and the stirring time was 1h to fully dissolve; Step 2: Add 30g of water and 1.0% by mass of phospholipase A1 to the dissolved soybean phospholipid oil solution, place it in a constant temperature magnetic stirrer, enzymatic hydrolysis temperature 50℃, rotation speed 30rpm, enzymatic hydrolysis time 9h, after the reaction is completed, heat at 85℃ for 20min to inactivate phospholipase, and obtain soybean phospholipid hydrolysis product. Step 3: Perform vacuum rotary distillation on the soybean phospholipid enzymatic hydrolysis product at a temperature of 70℃, a rotation speed of 150 rpm, a pressure of 0.05 MPa, and a distillation time of 3 hours to obtain a concentrated solution. Step 4: Add 60 mL of acetone to the concentrate and dissolve it completely. Centrifuge at 10,000 rpm for 5 min. Collect the precipitate after centrifugation. Add another 60 mL of acetone to the precipitate and dissolve it completely. Centrifuge at 10,000 rpm for 5 min. Collect the precipitate after centrifugation and dry it with hot air at 40℃ for 1 h. Step 5: Add 80 mL of water to the precipitate and dissolve it completely. Centrifuge at 10,000 rpm for 20 min. After centrifugation, collect the precipitate, add another 80 mL of water to the precipitate and dissolve it completely. Centrifuge at 10,000 rpm for 20 min. After centrifugation, collect the precipitate and dry it with hot air at 40℃ for 8 h to obtain glycolipid compounds.

[0031] Comparative Example 1: This comparative example is basically the same as Example 1, except that 2g of sunflower lecithin is used instead of 2g of soybean lecithin.

[0032] The glycolipid compounds obtained in this comparative example were analyzed by HPLC-ELSD, and the results are as follows: Figure 4 As shown, after testing and calculation, the purity of ASG was 0.76%, the purity of SG was 38.40%, and the purity of DGDG was 0.71%; the recovery rate of ASG was 0.28%, the recovery rate of SG was 14.21%, and the recovery rate of DGDG was 0.26%; the purity of glycolipid compounds was 39.87%, the recovery rate of glycolipid compounds was 14.75%, and the yield of glycolipid compounds was 4.11%.

[0033] Comparing the test results of Example 1 and Comparative Example 1, it can be seen that using sunflower lecithin, commonly used in the prior art, instead of soybean lecithin for the extraction of glycolipid compounds, resulted in no detected CE in the obtained glycolipid products, with the main components being ASG, SG, and DGDG. Compared with Example 1, DGDG appeared in the product, but its purity and recovery rate were both lower; at the same time, the purity, recovery rate, and glycolipid compound yield of ASG and SG also decreased, indicating that the method of the present invention, using soybean lecithin for the extraction of glycolipid compounds, significantly improves the purity, recovery rate, and yield of the obtained product.

[0034] Comparative Example 2: This comparative example is basically the same as Example 1, except that step 5, i.e., the water washing step, was not performed.

[0035] The sugar lipid compound obtained in the present comparative example was detected by HPLC-ELSD, and the results are shown in Table 2. Figure 5 As shown in Table 2, the purity of ASG was 0.40%, the purity of SG was 0.98%, the purity of CE was 0.41%; the recovery rate of ASG was 3.28%, the recovery rate of SG was 7.97%, the recovery rate of CE was 3.36%; the purity of sugar lipid compound was 1.79%, the recovery rate of sugar lipid compound was 14.61%, and the yield of sugar lipid compound was 90.66%.

[0036] As can be seen from the test results of Comparative Example 1 and Comparative Example 2, the recovery rate of ASG has a small increase without water washing treatment of the precipitate after acetone treatment, but the other indicators are all decreased, which shows that the water washing treatment of the final product in the method of the present application greatly improves the purity, recovery rate and yield of the obtained product.

[0037] Comparative Example 3: The present comparative example is basically the same as Example 1, except that the precipitate is dissolved with the same volume of n-hexane instead of water in step 5.

[0038] The sugar lipid compound obtained in the present comparative example was detected by HPLC-ELSD, and the results are shown in Table 2. Figure 6 As shown in Table 2, the purity of ASG was 0.40%, the purity of SG was 0.98%, the purity of CE was 0.41%; the recovery rate of ASG was 3.28%, the recovery rate of SG was 7.97%, the recovery rate of CE was 3.36%; the purity of sugar lipid compound was 1.79%, the recovery rate of sugar lipid compound was 14.61%, and the yield of sugar lipid compound was 90.66%.

[0039] As can be seen from the test results of Comparative Example 1 and Comparative Example 3, the precipitate is dissolved with n-hexane instead of water, and the main components of the obtained sugar lipid product are ASG, SG, CE and DGDG. Compared with Example 1, DGDG appears in the product, but the purity and recovery rate are both low; at the same time, the recovery rate of ASG and the yield of sugar lipid compound are both increased, but the other indicators are all decreased, which shows that the water washing treatment of the final product in the method of the present application greatly improves the purity and recovery rate of the obtained product.

Claims

1. A method for extracting glycolipid compounds from soybean lecithin, characterized in that, The specific steps are as follows: Step 1: Add soybean lecithin granules to soybean oil and stir until the soybean lecithin granules are fully dissolved; Step 2: Add water and phospholipase to the dissolved soybean phospholipid oil solution, stir to allow the enzymatic hydrolysis reaction to occur, and heat to inactivate the phospholipase after the reaction is completed to obtain the soybean phospholipid hydrolysis product. Step 3: Distill the soybean phospholipid hydrolysis product to obtain a concentrated solution; Step 4: Add acetone to the concentrate to fully dissolve it, centrifuge, collect the precipitate and dry it; Step 5: Add water to the precipitate obtained in step 4 to fully dissolve the precipitate, centrifuge, collect the precipitate and dry it to obtain glycolipid compounds.

2. The method for extracting glycolipid compounds from soybean lecithin according to claim 1, characterized in that, In step 1, the mass ratio of soybean phospholipid granules to soybean oil is 1:5-10.

3. The method for extracting glycolipid compounds from soybean lecithin according to claim 1, characterized in that, In step 1, the stirring temperature is 75-85℃, the stirring speed is 30-60rpm, and the stirring time is 1-4h.

4. The method for extracting glycolipid compounds from soybean lecithin according to claim 1, characterized in that, In step 2, the mass ratio of water to soybean phospholipid granules is 15-25:1, the phospholipase is phospholipase A1, and the enzyme concentration is 0.2-1.4% of the total mass of the reactants.

5. The method for extracting glycolipid compounds from soybean phospholipids according to claim 1, characterized in that, In step 2, the stirring temperature is 30-55℃, the stirring speed is 30-60 rpm, and the enzymatic hydrolysis reaction time is 1-9h. After the reaction is completed, heat to 85-95℃ and keep for 5-20min to inactivate the phospholipase.

6. The method for extracting glycolipid compounds from soybean lecithin according to claim 1, characterized in that, Step 3 specifically involves: The soybean phospholipid enzymatic hydrolysate was concentrated by vacuum rotary distillation at a temperature of 70-95℃, a rotation speed of 50-150 rpm, a pressure of 0.05-0.1 MPa, and a distillation time of 3-10 h. The concentrated solution was obtained after distillation.

7. The method for extracting glycolipid compounds from soybean lecithin according to claim 1, characterized in that, In step 4, the amount of acetone added is 20-30 mL per 1 g of soybean lecithin granules.

8. The method for extracting glycolipid compounds from soybean lecithin according to claim 1, characterized in that, In step 4, the steps of adding acetone, centrifuging, and collecting the precipitate are performed twice. The centrifugation speed is 8000-10000 rpm and the centrifugation time is 5-20 min. The drying is done by hot air drying at a temperature of 35-50℃ for 0.5-2 h.

9. The method for extracting glycolipid compounds from soybean lecithin according to claim 1, characterized in that, In step 5, the mass ratio of the added water to the soybean lecithin granules is 30-40:

1.

10. The method for extracting glycolipid compounds from soybean lecithin according to claim 1, characterized in that, In step 5, the steps of adding water, centrifuging, and collecting the precipitate are performed twice. The centrifugation speed is 8000-10000 rpm and the centrifugation time is 20-40 min. The drying is done by hot air drying at a temperature of 40-60℃ for 4-10 h.

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