Medicinal and edible traditional Chinese medicine microbial fermentation compound and feed application

Abstract

The invention relates to the technical field of feed additives, and particularly discloses a medicinal and edible traditional Chinese medicine microbial fermentation compound and feed application, the fermentation compound is prepared by taking astragalus membranaceus, codonopsis pilosula, poria cocos, bighead atractylodes rhizome, angelica sinensis, ligusticum wallichii and liquorice as medicinal and edible traditional Chinese medicine components, matching with a microbial fermentation agent, a compound enzyme, a carbon source and water in a specific proportion, and carrying out synergistic fermentation. Through the synergistic effect of the compound enzyme and microorganisms, the lignocellulose structure of the traditional Chinese medicine cell walls is destroyed, and the release rate of effective components is remarkably increased; through compounding of the saccharomyces cerevisiae culture, uniform mixing with the feed is realized, and the technical problems of low utilization rate and non-uniform mixing of a traditional Chinese medicine feed additive are solved. The beef cattle feed using the fermented compound can significantly improve the immunity, disease resistance, feed conversion rate and weight gain performance of beef cattle, reduces the use of antibiotics, ensures the food safety of beef, and is suitable for the large-scale beef cattle breeding industry.

Description

Technical Field

[0001] This invention relates to the fermentation complex of medicinal and edible Chinese herbal medicines by microorganisms and its application in feed, and is specifically a fermentation complex of medicinal and edible Chinese herbal medicines by microorganisms, its preparation method, and its application in beef cattle feed. Background Technology

[0002] With the large-scale and intensive development of my country's livestock farming industry, the beef cattle industry, as an important part of animal husbandry, faces multiple demands, including improving breeding efficiency, ensuring product quality, and protecting the environment. Traditional Chinese medicine, which is both food and medicine, has unique advantages in replacing veterinary antibiotics and achieving healthy livestock farming due to its naturalness, safety, and multifunctionality, and has become a research hotspot in the field of animal husbandry.

[0003] Astragalus, Codonopsis, Poria, Atractylodes macrocephala, Angelica sinensis, Ligusticum chuanxiong, and Glycyrrhiza uralensis are all traditional Chinese medicinal herbs that are both food and medicine, possessing rich bioactive components and well-defined pharmacological effects. Among them, Astragalus polysaccharides, saponins, and flavonoids in Astragalus can enhance the body's immune function, promote cell metabolism, and have antioxidant effects; Codonopsis polysaccharides and saponins in Codonopsis can regulate gastrointestinal motility, enhance immunity, and improve blood rheology; Poria polysaccharides and pachymic acid in Poria have immunomodulatory, anti-tumor, diuretic, and liver-protective effects; volatile oils and polysaccharides in Atractylodes macrocephala can invigorate the spleen and replenish qi, enhance immunity, and delay aging; volatile oils, vitamins, and minerals in Angelica sinensis are good for nourishing blood, regulating uterine function, and promoting bowel movements; ligustrazine and ferulic acid in Ligusticum chuanxiong can dilate blood vessels, prevent thrombosis, and have analgesic and anti-inflammatory effects; and glycyrrhizic acid and glycyrrhizin flavonoids in Glycyrrhiza uralensis have anti-inflammatory, antiviral, and immunomodulatory effects. Applying these medicinal and edible herbs to beef cattle feed can not only enhance the cattle's disease resistance but also endow beef with certain health benefits, meeting consumers' demand for high-quality meat products. Based on this, developing a beef cattle industry based on traditional Chinese medicine, creating distinctive beef brands using traditional Chinese medicine, and achieving the industrial goals of "technological support, agricultural-livestock integration, increased industrial efficiency, and increased farmer income" has become an important direction for the transformation and upgrading of the beef cattle industry.

[0004] The following problems still exist with existing technologies: 1. The current method is to directly crush Chinese medicine and mix it into feed. The cell walls of Chinese medicine are composed of lignocellulose, and the active ingredients are encapsulated in them. The gastrointestinal tract of beef cattle has difficulty digesting and breaking down lignocellulose, which results in most of the active ingredients not being released and absorbed, causing a waste of Chinese medicine resources and failing to fully exert their health benefits. 2. The physical properties of traditional Chinese medicine powder and feed base are different. When mixed directly, agglomeration and stratification are likely to occur, resulting in inconsistent dosage of traditional Chinese medicine ingested by individual beef cattle. Some beef cattle may not get enough to achieve the health benefits, while others may ingest too much and cause metabolic burden. At the same time, traditional beef cattle feed often relies on antibiotics to prevent diseases. Long-term use can easily lead to problems such as antibiotic residues in beef and increased bacterial resistance. Summary of the Invention

[0005] The purpose of this invention is to provide a microbial fermentation complex of traditional Chinese medicine that is both food and medicine and its application in feed, so as to solve the problems mentioned in the background art, such as the effective components of traditional Chinese medicine being encapsulated by lignocellulose, low release rate, and insufficient utilization rate when directly added to feed, as well as the problems of poor uniformity of mixing Chinese medicine with feed, unstable application effect, and food safety hazards caused by antibiotic dependence in feed.

[0006] To solve the above-mentioned technical problems, the present invention relates to a microbial fermentation complex of medicinal and edible herbs and its application in feed, which is achieved through the following technical solution: In a first aspect, the present invention provides a technical solution: a microbial fermentation complex of medicinal and edible herbs, the complex being composed of the following components in percentage: 20%-35% medicinal and edible herbs, 0.3%-0.8% microbial fermentation agent, 0.2%-0.5% compound enzyme, 3%-6% carbon source, and the remainder being water.

[0007] The medicinal and edible herbal ingredients consist of Astragalus membranaceus, Codonopsis pilosula, Poria cocos, Atractylodes macrocephala, Angelica sinensis, Ligusticum chuanxiong, and Glycyrrhiza uralensis, with each ingredient in a weight ratio of (1.5-2.5):(0.8-1.2):(0.8-1.2):(0.8-1.2):(0.8-1.2):(2.0-2.8).

[0008] The microbial fermentation agent includes Saccharomyces cerevisiae, Lactobacillus acidophilus, Bacillus subtilis, and Clostridium butyricum, wherein: The viable count of *Saccharomyces cerevisiae* was 1.5 × 10⁻⁶. 11 -2.5×10 11 CFU / g.

[0009] The viable count of Lactobacillus acidophilus was 0.8 × 10⁸. 11 -1.5×10 11 CFU / g.

[0010] The viable count of Bacillus subtilis was 0.8 × 10⁸. 12 -1.2×10 12 CFU / g.

[0011] The viable count of Clostridium butyricum was 0.8 × 10⁸. 11 -1.2×1011 CFU / g.

[0012] The complex enzyme is a mixture of acidic cellulase and acidic xylanase, with a weight ratio of 1:1 to 3:1.

[0013] Furthermore, the weight ratio of the medicinal and edible herbal components is Astragalus membranaceus: Codonopsis pilosula: Poria cocos: Atractylodes macrocephala: Angelica sinensis: Ligusticum chuanxiong: Glycyrrhiza uralensis = 2:1:1:1:1:1:2.6.

[0014] Furthermore, the carbon source is cane brown sugar, and the moisture content of the cane brown sugar is ≤8% and the purity is ≥95%.

[0015] Furthermore, the acid cellulase activity in the complex enzyme is ≥5000 U / g.

[0016] The enzyme activity of the acidic xylanase is ≥8000 U / g.

[0017] The enzyme activity assay conditions are pH 4.0-5.0 and temperature 40℃-50℃.

[0018] Secondly, according to the first aspect above, the present invention also provides a technical solution: the application of a microbial fermentation complex of medicinal and edible herbs in beef cattle feed, wherein the fermentation complex is added to the basic feed of beef cattle after being compounded with auxiliary materials as a feed additive, and the mass fraction of the fermentation complex in the beef cattle feed is 0.4%-1.2%.

[0019] Furthermore, the auxiliary material is a culture of brewer's yeast, and the mass ratio of the fermentation complex to the culture of brewer's yeast is 1:8-1:10, which, after compounding, yields a mixed feed additive.

[0020] Furthermore, the beef cattle feed consists of a base feed and a mixed feed additive, wherein the base feed accounts for 96%-98% of the dry matter of the beef cattle feed, and the mixed feed additive accounts for 2%-4% of the dry matter of the beef cattle feed.

[0021] Furthermore, the base feed comprises, by dry matter weight percentage: 35%-45% corn, 15%-20% soybean meal, 8%-12% DDGS, 8%-12% corn germ meal, 8%-12% rice bran meal, 5%-7% brewing culture, 0.5%-1.5% zeolite powder, and 4%-6% mineral and vitamin premix feed.

[0022] Thirdly, based on the first aspect above, the present invention further provides a technical solution: a method for preparing a microbial fermentation complex of medicinal and edible herbs, comprising the following steps: (1) Raw material pretreatment: The medicinal and edible Chinese medicine components are pulverized to 80-120 mesh, sieved and dried until the moisture content is ≤13%.

[0023] (2) Mixing ingredients: Mix the pretreated Chinese medicine components with carbon source and water evenly, adjust the moisture content of the system to 55%-65%, then add microbial fermentation agent and compound enzyme, and stir until evenly mixed.

[0024] (3) Sealed fermentation: Place the mixture in a fermentation tank and seal it for 15-25 days at 30℃-45℃ and relative humidity of 60%-70%. Stir once every 3-5 days during the period, and each stirring time is 15-30 minutes. The pressure inside the fermentation tank is controlled at 0.05-0.1MPa.

[0025] (4) Post-processing: After fermentation, the fermentation product is dried at a low temperature of 45℃-55℃ until the moisture content is ≤10%, and then pulverized to 60-80 mesh to obtain the fermentation complex.

[0026] (5) Product testing: Test the content of effective ingredients, the number of viable microorganisms and the moisture content in the fermentation complex in step (4).

[0027] Furthermore, the ash content of the medicinal and edible components described in step (1) is ≤5%, and the content of the effective components of each medicinal herb complies with the current version of the Pharmacopoeia of the People's Republic of China.

[0028] In step (3), a spiral stirring method is used, and the stirring speed is 30-50 r / min.

[0029] The present invention has the following beneficial effects: (1) The present invention uses a complex enzyme composed of acidic cellulase and acidic xylanase to first break the connection between lignin and hemicellulose in the cell wall of Chinese medicine. Then, combined with the synergistic decomposition of cellulose and xylan by microorganisms such as Bacillus subtilis and Lactobacillus acidophilus, the cell wall structure is completely broken, so that the effective components such as Astragalus polysaccharide, Codonopsis saponin and Poria polysaccharide that are encapsulated are fully released. According to the test, the release rate of effective components is more than 35% higher than that of the traditional direct pulverization and addition method, which greatly improves the utilization rate of Chinese medicine resources.

[0030] (2) In the microbial fermentation process of the present invention, Saccharomyces cerevisiae converts the sugars in traditional Chinese medicine into metabolites such as ethanol and organic acids, Lactobacillus acidophilus produces lactic acid to regulate the fermentation environment, and Clostridium butyricum produces butyric acid and other bioactive substances. These metabolites work synergistically with the effective components of traditional Chinese medicine, which not only enhances the effects of immune regulation, anti-inflammation and anti-oxidation, but also reduces the anti-nutritional factors in traditional Chinese medicine, and improves the safety and bioavailability of the complex.

[0031] (3) The present invention uses brewer's yeast culture as an excipient compound, and utilizes its porous structure and dispersion characteristics to make the fermentation complex uniformly dispersed in the feed, avoiding agglomeration and stratification. The test results show that the coefficient of variation of the uniformity of the mixed feed additive in the base feed is ≤5%, ensuring that the dosage of the effective Chinese medicine ingested by each beef cattle is consistent and ensuring the stability of the application effect.

[0032] (4) The effective components of traditional Chinese medicine and microbial metabolites of the present invention can work synergistically to upregulate the expression of anti-inflammatory factors in beef cattle, enhance macrophage activity and lymphocyte transformation ability, improve intestinal barrier function, reduce transportation stress response and disease incidence in beef cattle. In the experiment, the mortality rate of calves transported to the farm within 0-30 days was reduced by 83.3% compared with the control group, significantly improving the survival rate of breeding.

[0033] (5) This invention adopts the principle of food and medicine homology, and prepares natural plant-based Chinese medicine into feed additives after microbial fermentation. It is applied to beef cattle breeding, aiming to improve the immunity, feed conversion rate and weight gain performance of beef cattle, reduce the use of antibiotics, and achieve green and healthy breeding.

[0034] Of course, any product implementing this invention does not necessarily need to achieve all of the advantages described above at the same time. Attached Figure Description

[0035] To more clearly illustrate the technical solutions of the embodiments of the present invention, the accompanying drawings used in the description of the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0036] Figure 1 This is a flowchart of the preparation method of the present invention. Detailed Implementation

[0037] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0038] Please see Figure 1 As shown, this invention relates to a microbial fermentation complex of medicinal and edible herbs and its application in feed.

[0039] In a first aspect, the present invention provides a microbial fermentation complex of medicinal and edible herbs, the complex being composed of the following components in percentage: 20%-35% medicinal and edible herbs, 0.3%-0.8% microbial fermentation agent, 0.2%-0.5% compound enzyme, 3%-6% carbon source, and the balance being water; The medicinal and edible herbs are composed of Astragalus membranaceus, Codonopsis pilosula, Poria cocos, Atractylodes macrocephala, Angelica sinensis, Ligusticum chuanxiong, and Glycyrrhiza uralensis, in a weight ratio of (1.5-2.5):(0.8-1.2):(0.8-1.2):(0.8-1.2):(0.8-1.2):(0.8-1.2):(2.0-2.8). The weight ratio of the medicinal and edible herbs is Astragalus membranaceus: Codonopsis pilosula: Poria cocos: Atractylodes macrocephala: Angelica sinensis: Ligusticum chuanxiong: Glycyrrhiza uralensis = 2:1:1:1:1:1:2.6. Microbial fermentation agents include Saccharomyces cerevisiae, Lactobacillus acidophilus, Bacillus subtilis, and Clostridium butyricum, among which: The viable count of *Saccharomyces cerevisiae* was 1.5 × 10⁻⁶. 11 -2.5×10 11 CFU / g; The viable count of Lactobacillus acidophilus was 0.8 × 10⁸. 11 -1.5×10 11 CFU / g; The viable count of Bacillus subtilis was 0.8 × 10⁸. 12 -1.2×10 12 CFU / g; The viable count of Clostridium butyricum was 0.8 × 10⁸. 11 -1.2×10 11 CFU / g; The complex enzyme is a mixture of acidic cellulase and acidic xylanase in a weight ratio of 1:1 to 3:1, wherein the enzyme activity of acidic cellulase is ≥5000U / g; The enzyme activity of acidic xylanase is ≥8000 U / g; The enzyme activity assay conditions were pH 4.0-5.0 and temperature 40℃-50℃.

[0040] The carbon source is cane brown sugar, which has a moisture content of ≤8% and a purity of ≥95%.

[0041] Secondly, the application of microbial fermentation complexes of medicinal and edible herbs in beef cattle feed involves adding the fermentation complex as a feed additive to the basic beef cattle feed. The fermentation complex accounts for 0.4%-1.2% of the beef cattle feed by mass. The auxiliary material is a culture of brewer's yeast, with a mass ratio of 1:8-1:10 between the fermentation complex and the brewer's yeast culture. After compounding, a mixed feed additive is obtained. The beef cattle feed consists of a basic feed and a mixed feed additive. The basic feed accounts for 96%-98% of the dry matter of the beef cattle feed, and the mixed feed additive accounts for 2%-4% of the dry matter of the beef cattle feed. The basic feed, by dry matter weight percentage, includes: corn 35%-45%, soybean meal 15%-20%, DDGS 8%-12%, corn germ meal 8%-12%, rice bran meal 8%-12%, brewer's yeast culture 5%-7%, zeolite powder 0.5%-1.5%, and mineral and vitamin premix 4%-6%.

[0042] Thirdly, the preparation method of microbial fermentation complex of medicinal and edible herbs includes the following steps: (1) Raw material pretreatment: The medicinal and edible Chinese herbal medicine components are pulverized to 80-120 mesh, sieved and dried until the moisture content is ≤13% and the ash content of the medicinal and edible Chinese herbal medicine components is ≤5%; (2) Mixing ingredients: Mix the pretreated Chinese medicine components with carbon source and water evenly, adjust the moisture content of the system to 55%-65%, then add microbial fermentation agent and compound enzyme, and stir until evenly mixed; (3) Sealed fermentation: Place the mixture in a fermentation tank and seal it for 15-25 days at 30℃-45℃ and relative humidity of 60%-70%. Stir once every 3-5 days during the period, and each stirring time is 15-30 minutes. The pressure in the fermentation tank is controlled at 0.05-0.1MPa. The stirring is done by spiral stirring at a speed of 30-50r / min. (4) Post-processing: After fermentation, the fermentation product is dried at a low temperature of 45℃-55℃ until the moisture content is ≤10%, and then pulverized to 60-80 mesh to obtain the fermentation complex. (5) Product testing: Test the content of effective ingredients, the number of viable microorganisms and the moisture content in the fermentation complex in step (4).

[0043] Enzymatic hydrolysis and synergistic release of active ingredients from traditional Chinese medicine: The main component of the cell wall of traditional Chinese medicine is lignocellulose (lignin, cellulose, hemicellulose), within which the active ingredients are encapsulated. In this invention, the complex enzyme consists of acidic cellulase and acidic xylanase. It first acts on the cell wall, with acidic xylanase decomposing xylan in hemicellulose and acidic cellulase decomposing cellulose. Simultaneously, the oxidase component in the complex enzyme oxidizes lignin, disrupting the connection structure between lignin and hemicellulose / cellulose, thus opening cell wall channels. Subsequently, Bacillus subtilis, as a cellulose-decomposing bacterium, further decomposes the remaining cellulose and hemicellulose. Lactobacillus acidophilus synergistically decomposes xylan with the enzyme. Saccharomyces cerevisiae consumes oxygen in the fermentation system, forming an anaerobic microenvironment that provides conditions for the proliferation of Clostridium butyricum. Clostridium butyricum further promotes the dissolution and transformation of the traditional Chinese medicine components through acid and enzyme production, ultimately achieving the full release of the active ingredients.

[0044] Microbial fermentation enhances the bioactivity of the complex: During fermentation, various microbial strains produce a variety of bioactive substances through metabolic activities, which synergistically enhance the efficacy of the complex with the effective components of traditional Chinese medicine. Under anaerobic conditions, *Saccharomyces cerevisiae* converts sugars in traditional Chinese medicine into metabolites such as ethanol, organic acids, and amino acids. Ethanol promotes the dissolution of fat-soluble effective components, while organic acids adjust the pH of the fermentation system to acidic (pH 4.5-5.5), adapting to the acidic environment of the rumen in beef cattle and improving the stability and absorption rate of effective components in the rumen. Lactic acid produced by *Lactobacillus acidophilus* further acidifies the environment, inhibiting the growth of harmful microorganisms, while its metabolites regulate the balance of intestinal flora. Butyric acid produced by *Clostridium butyricum* provides energy for intestinal mucosal cells, promotes mucin secretion, and enhances intestinal barrier function. Its butyric acid clostridium butyricum inoculin can inhibit contamination by molds and other bacteria, reducing the risk of aflatoxin formation. Simultaneously, fermentation products can activate the TLR2 signaling pathway in beef cattle, upregulating the expression of anti-inflammatory factors (such as IL-10) and downregulating the expression of pro-inflammatory factors (such as IL-6 and TNF-α), thus enhancing immune regulation.

[0045] The compound carrier improves the uniformity of feed mixing: The fermentation complex is in powder form, which is prone to agglomeration when directly mixed with the base feed, resulting in uneven mixing. This invention uses brewer's yeast culture as an excipient, which has a porous structure and good dispersibility. When compounded with the fermentation complex at a ratio of 1:9, the fermentation complex particles are adsorbed on the porous surface of the brewer's yeast culture, forming a stable dispersion system and avoiding agglomeration. When mixed with the base feed, the brewer's yeast culture acts as a carrier, driving the fermentation complex to be evenly dispersed in the base feed, reducing the coefficient of variation of mixing uniformity, ensuring that the effective component dosage ingested by each beef cattle is consistent, and ensuring the stability of the application effect.

[0046] Feed application regulates the physiological functions of beef cattle: After beef cattle feed with added fermentation complex is consumed, the mixed feed additive enters the rumen. The beneficial microorganisms (Saccharomyces cerevisiae, Lactobacillus acidophilus, Bacillus subtilis, Clostridium butyricum) colonize the rumen, regulate the rumen flora balance, improve the fermentation efficiency of rumen microorganisms, enhance rumen rumination capacity and digestive enzyme activity, and promote the digestion and absorption of nutrients such as corn and soybean meal in the feed. After the effective components of traditional Chinese medicine (Astragalus polysaccharide, Codonopsis pilosula polysaccharide, etc.) and microbial metabolites (butyric acid, amino acids, etc.) in the fermentation complex are absorbed, they act on the beef cattle's immune system, enhance the phagocytic capacity of macrophages and the transformation capacity of lymphocytes, increase antibody production levels, and enhance the body's immunity and disease resistance. At the same time, the effective components and metabolites can also regulate the metabolism of beef cattle, promote protein synthesis and fat deposition, improve weight gain performance, and shorten the slaughter cycle.

[0047] In summary, this invention solves the problem of low utilization rate of effective components in traditional Chinese medicine feed additives through enzymatic hydrolysis and microbial synergistic fermentation technology; it solves the problem of poor mixing uniformity through the compounding of brewer's yeast culture; and it replaces some antibiotics through the synergistic effect of Chinese medicine and microorganisms, thus solving food safety risks. Ultimately, it achieves green and healthy cattle breeding, improving breeding efficiency and industrial competitiveness.

[0048] Example 1: Preparation of microbial fermentation complex of medicinal and edible herbs The raw materials are formulated by weight percentage as follows: 28% of medicinal and edible herbs (Astragalus membranaceus 100kg, Codonopsis pilosula 50kg, Poria cocos 50kg, Atractylodes macrocephala 50kg, Angelica sinensis 50kg, Ligusticum chuanxiong 50kg, Glycyrrhiza uralensis 130kg, weight ratio 2:1:1:1:1:1:2.6), 0.54% of microbial fermentation agent (Saccharomyces cerevisiae 1.0kg, Lactobacillus acidophilus 0.5kg, Bacillus subtilis 0.2kg, Clostridium butyricum 1.0kg), 0.26% of compound enzymes (acid cellulase 0.67kg, acid xylanase 0.33kg, weight ratio 2:1), 4% of carbon source (cane brown sugar 20kg), and 67.2% of water (water 500kg).

[0049] Preparation steps of microbial fermentation complex of medicinal and edible herbs: (1) Raw material pretreatment: The above seven kinds of Chinese medicines were pulverized to 100 mesh using a WFJ-15 type micro pulverizer, passed through an 80 mesh sieve, and then placed in a CT-C-II type hot air circulating drying oven at 60°C for 2 hours. The moisture content after drying was 11%. (2) Mixing ingredients: Add the dried Chinese herbal medicine components and sugarcane brown sugar to a 500L mixer, add 500kg of water, stir for 10 minutes to mix evenly, adjust the moisture content of the system to 60%, then add microbial fermentation agent and compound enzyme, stir at 40r / min for 15 minutes to ensure even mixing; (3) Sealed fermentation: Transfer the mixture to a 500L stainless steel fermentation tank, seal it, and control the fermentation temperature at 38℃, relative humidity at 65%, and pressure inside the tank at 0.08MPa. Ferment for 18 days, stirring at 40r / min for 20 minutes every 4 days during this period. (4) Post-processing: After fermentation, the fermentation product is placed in a 50℃ low-temperature drying equipment and dried to a moisture content of 8%. Then it is crushed to 70 mesh, passed through a 60 mesh sieve, sealed and packaged to obtain the medicinal and edible herbal microbial fermentation complex.

[0050] (5) Product testing: a. Content of effective ingredients: Astragalus polysaccharide ≥10.5%, Codonopsis polysaccharide ≥7.8%, Poria polysaccharide ≥13.2%, which is 31%-35% higher than the content of effective ingredients in the raw materials; a. Viable microbial count: Total viable count ≥ 5 × 10⁻⁶ 10 CFU / g, of which Saccharomyces cerevisiae ≥2×10 10 CFU / g, Lactobacillus acidophilus ≥1×10 9 CFU / g, Bacillus subtilis ≥2×10 10 CFU / g, Clostridium butyricum ≥1×10 9 CFU / g; c. Moisture content: 8%, ash content: 4.2%, which meets the quality requirements.

[0051] Example 2: Application and efficacy verification of microbial fermentation complex of medicinal and edible herbs in beef cattle feed.

[0052] Experimental materials: Mixed feed additive: The fermentation complex prepared in Example 1 was mixed with the brewer's yeast culture at a mass ratio of 1:9 and mixed for 20 minutes using a DLH-50 double helix conical mixer to obtain the mixed feed additive. Base feed: Prepare the base feed according to the dry matter weight percentage, including 40% corn, 18% soybean meal, 10% DDGS, 10% corn germ meal, 10% rice bran meal, 6% brewing culture, 1% zeolite powder, and 5% mineral and vitamin premix feed. Mix evenly and set aside. Beef cattle feed: Experimental Group 1: 98% basic feed plus 2% mixed feed additive (0.2% by mass of fermentation complex in feed); Experimental Group 2: 96% basic feed plus 4% mixed feed additive (0.4% by mass of fermentation complex in feed); Control group: 100% base material (no fermentation complex and antibiotics added).

[0053] Experimental Design: Experimental animals: 900 healthy calves weighing about 500 catties were selected and randomly divided into control group, experimental group 1 and experimental group 2, with 300 calves in each group. There was no significant difference in the initial weight among the groups (P>0.05). Experimental location: A large-scale beef cattle breeding base was selected; Trial period: 300 days (0-30 days is the adaptation period, 30-300 days is the fattening period). Feeding method: All groups are allowed free access to feed and water, and are fed 3 times a day. Feed troughs and water troughs are cleaned regularly to keep the breeding environment clean. Testing indicators: average weight upon entry, average weight at different stages, feed intake, number of deaths, net weight gain, and feed conversion ratio.

[0054] Experimental results: Table 1. Trial data of calves transported to the farm from 0 to 30 days. detection indicators control group Experimental group 1 Experimental group 2 Number of heads (heads) 300 300 300 Average weight at entry into the pen (in kilograms) 510 525 505 Average weight at 30 days (catties / head) 600 645 640 Dead search number (head) 6 2 1 Dead rate (%) 2.0 0.7 0.3 Average feed intake (catties / head) 198 225 260 Average net weight gain (catties / head) over 30 days 90 120 135 Feed conversion ratio (feed intake / net weight gain) 2.2:1 1.87:1 1.93:1 Table 2. Data from the 30-300 day post-entry trial on the market. detection indicators control group Experimental group 1 Experimental group 2 Number of heads (heads) 294 298 299 Average weight at 30 days (catties / head) 600 645 640 Average weight at slaughter (catties / head) 1350 1495 1480 Dead search number (head) 0 0 0 Average feed intake (catties / head) 5452 5375 5400 Net weight gain during fattening period (catties / head) 750 850 840 Feed conversion ratio during the fattening period (feed intake / net weight gain) 7.27:1 6.32:1 6.43:1 Table 3 Summary of test data for the entire cycle (0-300 days) detection indicators control group Experimental group 1 Experimental group 2 Net weight gain over the entire cycle (catties / head) 840 970 975 Average daily weight gain (catties / head) throughout the entire cycle 2.8 3.23 3.25 Average feed intake over the entire cycle (catties / head) 5650 5600 5660 Feed conversion ratio over the entire life cycle (feed intake / net weight gain) 6.73:1 5.77:1 5.81:1 Survival rate (%) 98.0 99.3 99.7 Results analysis: Health status: The mortality rate of experimental groups 1 and 2 was significantly lower than that of the control group. The mortality rate from 0 to 30 days was reduced by 65% ​​and 85% respectively, and the survival rate throughout the cycle was over 99%, indicating that the fermentation complex can effectively enhance the immunity and stress resistance of beef cattle and reduce the incidence of disease.

[0055] Weight gain performance: The average daily weight gain of experimental groups 1 and 2 over the entire cycle was 15.4% and 16.1% higher than that of the control group, respectively, and the net weight gain was 15.5% and 16.1% higher, respectively, indicating that the fermentation complex can significantly improve the weight gain performance of beef cattle.

[0056] Feed conversion ratio: The feed conversion ratios of experimental groups 1 and 2 throughout the entire cycle were 14.3% and 13.7% higher than those of the control group, respectively, indicating that the fermentation complex can improve the digestive and absorptive functions of beef cattle and increase feed utilization efficiency.

[0057] Overall benefits: The feed addition of experimental group 1 was 2%, which showed excellent results in terms of weight gain, feed conversion rate and survival rate, and the cost was relatively low, making it the most cost-effective. The weight gain and survival rate of experimental group 2 were slightly better than those of experimental group 1, but the feed cost was higher. The appropriate addition amount can be selected according to the breeding needs.

[0058] The preferred embodiments of the present invention disclosed above are merely illustrative of the invention. These preferred embodiments do not exhaustively describe all details, nor do they limit the invention to the specific implementations described. Clearly, many modifications and variations can be made based on the content of this specification. This specification selects and specifically describes these embodiments to better explain the principles and practical applications of the invention, thereby enabling those skilled in the art to better understand and utilize the invention. The invention is limited only by the claims and their full scope and equivalents.

Claims

1. A medicinal and edible microorganism-fermented Chinese medicine compound, characterized in that, The compound is composed of the following components in percentage: 20%-35% of medicinal and edible Chinese medicine component, 0.3%-0.8% of microbial fermentation agent, 0.2%-0.5% of compound enzyme, 3%-6% of carbon source, and the balance of water; The medicinal and edible Chinese medicine component is composed of Huangqi, Dangshen, Fuling, Baishu, Dangui, Chuanxiong and Gancao, and each component is composed of (1.5-2.5):(0.8-1.2):(0.8-1.2):(0.8-1.2):(0.8-1.2):(0.8-1.2):(2.0-2.8) by weight ratio. The microbial fermentation agent comprises Saccharomyces cerevisiae, Lactobacillus acidophilus, Bacillus subtilis and Clostridium butyricum, wherein: The viable cell count of the Saccharomyces cerevisiae was 1.5 x 10 11 -2.5 x 10 11 CFU / g; The viable cell count of Lactobacillus acidophilus was 0.8 x 10 11 -1.5 x 10 11 CFU / g; The viable cell count of Bacillus subtilis was 0.8 x 10 12 -1.2 x 10 12 CFU / g; The viable cell count of Clostridium butyricum was 0.8 x 10 11 -1.2 x 10 11 CFU / g; The compound enzyme is a mixture of acid cellulase and acid xylanase, and the weight ratio of the two is 1:1-3:

1.

2. The pharmaconutritional traditional Chinese medicine microbial fermentation compound according to claim 1, characterized in that, The weight ratio of the medicinal and edible Chinese medicine component is Huangqi:Dangshen:Fuling:Baishu:Dangui:Chuanxiong:Gancao = 2:1:1:1:1:1:2.

6. 3.The TCMMMC of claim 1, characterized in that, The carbon source is sugarcane red sugar, and the moisture content of the sugarcane red sugar is ≤8% and the purity is ≥95%. 4.The TCMMMC of claim 1, characterized in that, The enzyme activity of the acid cellulase in the compound enzyme is ≥5000 U / g. The enzyme activity of the acid xylanase is ≥8000 U / g. The enzyme activity determination conditions are pH 4.0-5.0 and temperature 40-50°C.

5. The use of the medicinal and edible Chinese medicinal microbial fermentation compound in beef cattle feed, which adopts the medicinal and edible Chinese medicinal microbial fermentation compound in the above claims 1-4, characterized in that, The fermented compound is added to beef cattle basic feed after being compounded with auxiliary materials as a feed additive, and the mass fraction of the fermented compound in the beef cattle feed is 0.4%-1.2%.

6. The use of the homoisopharmaceutical traditional Chinese medicine microbial fermentation compound according to claim 5 in beef cattle feed, characterized in that, The auxiliary material is Saccharomyces cerevisiae culture, and the mass ratio of the fermented compound to the Saccharomyces cerevisiae culture is 1:8-1:10, and the mixed feed additive is obtained after compounding.

7. The use of the homoisopharmaceutical traditional Chinese medicine microbial fermentation compound according to claim 6 in beef cattle feed, characterized in that, The beef cattle feed is composed of basic material and mixed feed additive, wherein the basic material accounts for 96%-98% of the dry matter of the beef cattle feed, and the mixed feed additive accounts for 2%-4% of the dry matter of the beef cattle feed.

8. The use of the homoisopharmaceutical traditional Chinese medicine microbial fermentation compound according to claim 7 in beef cattle feed, characterized in that, The basic material includes, by weight percentage of dry matter: corn 35%-45%, soybean meal 15%-20%, DDGS 8%-12%, corn germ cake 8%-12%, rice bran meal 8%-12%, brewing culture 5%-7%, zeolite powder 0.5%-1.5%, and mineral and vitamin premixed feed 4%-6%.

9. The preparation method of the medicinal and edible traditional Chinese medicine microbial fermentation compound, which is applied to the medicinal and edible traditional Chinese medicine microbial fermentation compound in the above claims 1-4, characterized in that, The method comprises the following steps: (1) Raw material pretreatment: the medicinal and edible Chinese medicine component is crushed to 80-120 mesh, dried to a moisture content of ≤13% after sieving; (2) Mixing and batching: the pretreated Chinese medicine component is mixed and batched with the carbon source and water, the system moisture content is adjusted to 55%-65%, then the microbial fermentation agent and the compound enzyme are added, and stirring is performed until the mixture is uniformly mixed; (3) Sealed fermentation: the mixed material is placed in a fermentation tank, and is subjected to sealed fermentation at 30-45°C and relative humidity 60%-70% for 15-25 days, stirring is performed every 3-5 days during the fermentation, each stirring time is 15-30 minutes, and the pressure in the fermentation tank is controlled at 0.05-0.1 MPa; (4) Post-treatment: after the fermentation is completed, the fermentation product is dried at 45-55°C to a moisture content of ≤10%, and is crushed to 60-80 mesh to obtain the fermented compound; (5) Product detection: detecting the content of effective components, the number of active microorganisms and the moisture content in the fermentation complex in step (4).

10. The method of claim 9, wherein the preparation method of the TCM microbial fermentation compound is characterized in that, The ash content of the medicinal and edible Chinese medicinal component in step (1) is ≤5%; In step (3), the stirring adopts a spiral stirring mode, and the stirring speed is 30-50 r / min.

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