Perilla seed high-lecithin egg feed as well as preparation method and application thereof

Abstract

The invention relates to the field of feed, in particular to purple perilla seed high-lecithin egg feed and a preparation method and application thereof.The purple perilla seed high-lecithin egg feed comprises a basic feed and a functional additive, and the functional additive is prepared from, by weight, 50-100 parts of purple perilla seeds, 0.1-10 parts of choline sources, 0.05-0.1 part of vitamin E and 1 * 10 < 9 > CFU / kg of bacillus subtilis liquid; the basal feed is prepared from the following components in parts by weight: 60-65 parts of corn, 20-25 parts of soybean meal, 5-8 parts of bran, 3-5 parts of mountain flour, 1-2 parts of calcium hydrogen phosphate, 0.3-0.5 part of table salt and 1-2 parts of laying hen premix. The invention solves the problems of unprominent effect of perilla seeds, limited lecithin increase and poor component synergism in the prior art, and has the advantages of simple process, controllable cost and suitability for large-scale production.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of feed, in particular to a perilla seed high- lecithin chicken egg feed and a preparation method and application thereof. BACKGROUND

[0002] Lecithin is an essential nutrient for the human body, which has important physiological functions such as regulating blood lipids, improving memory, and protecting the liver. Egg yolk is a high-quality source of natural lecithin. With the upgrading of health consumption demand, improving the lecithin content in eggs through feed nutrition regulation technology to produce high-lecithin functional eggs has become a research hotspot in the industry.

[0003] In the prior art, there are related patents that attempt to improve the lecithin content of eggs through feed formula adjustment, but there are obvious defects: Chinese patent CN103948063A discloses a health egg feed, which complexly combines perilla seed with spirulina, ginkgo leaves and other ingredients. The core goal is to reduce cholesterol, and perilla seed is only an auxiliary ingredient, which does not highlight its dominant role in lecithin improvement, and the lecithin improvement range is only 10%-15%; Chinese patent CN106578361A discloses a feed rich in DHA and lecithin, which has complex ingredients and low perilla seed proportion (30g / kg). The perilla seed processing technology is not optimized, resulting in low utilization rate of nutritional ingredients and limited lecithin improvement effect. In addition, the prior art lacks quantitative verification of the "synergistic effect of ingredients", relies more on theoretical derivation, and has not formed a "core ingredient-technology optimization-synergistic effect" system, making it difficult to achieve a significant breakthrough in lecithin content.

[0004] Therefore, it is a technical problem to be solved in the field to develop a feed formula that takes perilla seed as the core, realizes efficient lecithin improvement through process optimization and precise compounding, and is supported by sufficient experimental data. SUMMARY

[0005] The present application aims to provide a perilla seed high-lecithin chicken egg feed and a preparation method and application thereof. By clarifying the dominant position of perilla seed, optimizing the processing technology, designing a multi-element synergistic system, and supplementing quantitative experimental data, the lecithin content of eggs is significantly and stably improved, while the industrial feasibility is also considered.

[0006] To achieve the above-mentioned purpose, in a first aspect, the present application provides a perilla seed high-lecithin chicken egg feed, which comprises a basic feed and a functional additive. The functional additive is composed of the following components in parts by weight: perilla seed 50-100 parts, choline source 0.1-10 parts, vitamin E 0.05-0.1 parts, Bacillus subtilis bacterial liquid 1x10 9The basic feed is composed of corn 60-65 parts, soybean meal 20-25 parts, bran 5-8 parts, stone powder 3-5 parts, calcium hydrogen phosphate 1-2 parts, salt 0.3-0.5 parts, and egg hen premix 1-2 parts by weight.

[0007] The vitamin E is DL-alpha-tocopherol acetate, and the viable bacterial count of the Bacillus subtilis is greater than or equal to 2*10 10 CFU / g.

[0008] The choline source is selected from one or more of choline chloride, egg yolk powder, and betaine.

[0009] The preparation method of the perilla seed high-ovophosphatidyl chicken egg feed comprises the following steps: After the perilla seeds are removed of impurities, the perilla seeds are crushed to 50-100 mesh by using a super micro grinder, Bacillus subtilis liquid is added, and fermentation is carried out at 30-35 DEG C for 24-48 hours; after the fermentation is completed, the perilla seed powder is obtained by drying at 60-70 DEG C until the water content is less than or equal to 12%. The perilla seed powder, the choline source, the vitamin E, and the Bacillus subtilis are mixed in proportion to obtain a functional compound; The corn, the soybean meal, the bran, the stone powder, the calcium hydrogen phosphate, the salt, and the egg hen premix are put into a mixer in proportion, mixed for 10-15 minutes, and uniformly mixed until the variation coefficient is less than or equal to 7% to obtain a basic mixed feed. The functional compound is added to the basic mixed feed, mixed at a speed of 20-25 rpm for 5-10 minutes, high-speed mixed at a speed of 60-70 rpm for 10-15 minutes, and finally mixed at a speed of 20-25 rpm for 5-8 minutes, and the variation coefficient of the uniform mixing is less than or equal to 5%; the mixed material is granulated by using a granulator at 80-85 DEG C, cooled to room temperature, and packaged to obtain a finished feed. In the fermentation process at 30-35 DEG C for 24-48 hours, the solid-liquid ratio is 1:0.8 (g / mL), the concentration of the Bacillus subtilis liquid is 1*10 8 CFU / mL, the mixture is stirred every 8 hours during the fermentation, and after the fermentation is completed, vacuum freeze-drying is carried out under the following drying conditions: cold trap temperature -50 DEG C, vacuum degree less than or equal to 10 Pa, and drying time 12-18 hours.

[0010] Before the mixed material is granulated by using a granulator at 80-85 DEG C, 0.1-0.3 wt% of a natural antioxidant solution is sprayed into the mixed material, and the natural antioxidant is selected from one or more of rosemary extract, tea polyphenol, or bamboo leaf flavone.

[0011] After the granulated feed is granulated by using a granulator at 80-85 DEG C, the granulated feed is subjected to surface coating treatment, the coating material is food-grade chitosan-sodium alginate composite film, and the coating thickness is 20-50 μm.

[0012] The functional compound is premixed with the carrier diatomite at a mass ratio of 1:0.5-1 before being added into the base mixed feed, so as to improve the uniformity of dispersion of the trace ingredients.

[0013] In a third aspect, the application provides an application of the perilla seed high-ovophosphatidyl egg feed, which is used for daily feeding of 180-300 day-old laying hens, the daily feeding amount is 110-120 g per hen, free water is provided, and the feeding is continuously performed for 30-60 days, and the ovophosphatidyl content in the egg yolk is stably maintained at 12-15.1 g / 100 g.

[0014] The perilla seed high-ovophosphatidyl egg feed, the preparation method and the application thereof have the following advantages: the ovophosphatidyl improving effect is remarkable and stable: through the combination of the perilla seed core, the choline source and the process optimization, the egg yolk ovophosphatidyl content is improved by 25%-54%, reaches 12-15.1 g per 100 g of egg yolk, and is much higher than the improvement range of 10%-15% in the prior art; the effect is not attenuated after continuous feeding for 60 days, and the stability is high.

[0015] The synergistic effect is quantitatively verified: the Bliss independent model is used for calculation, and the synergistic effect of the compounded ingredients is remarkable (the actual improvement effect is greater than the theoretical added value of the individual effect of each ingredient). For example, the perilla seed (80 g / kg) improves the ovophosphatidyl by 20%, the choline chloride (150 mg / kg) improves the ovophosphatidyl by 8%, the theoretical added value is 28%, the actual compounded improvement is 45.9%, and it is proved that the synergistic effect is obvious.

[0016] The perilla seed core plays an outstanding role: the perilla seed accounts for ≥70% in the functional additive, the bioavailability is improved by more than 40% through the combination of the ultrafine grinding and the fermentation pretreatment, the problem of poor effect of single perilla seed addition is solved, and the ingredients are simple, and the cost is controllable (reduced by 15%-20% compared with the existing complex compounded feed).

[0017] Both the production performance and the safety are considered: the egg laying rate of the laying hen after feeding is ≥90%, the egg weight, the eggshell strength and other indexes have no significant difference with those of the conventional feed; the cholesterol of the egg is reduced by 8%-10%, the heavy metal lead and cadmium are <0.01 mg / kg, meet the requirements of GB 2749-2015 Food Safety National Standard Eggs and Egg Products, and the safety is high.

[0018] The process is mature and easy to industrialize: the pretreatment and the mixing granulation process are mature technologies in the feed industry, no special equipment is needed, and the process can be directly adapted to the large-scale production line, and has a wide application prospect. BRIEF DESCRIPTION OF DRAWINGS

[0019] In order to make the technical solutions in the embodiments of the present application or the prior art clearer, the accompanying drawings needed in the embodiments or prior art description will be briefly introduced. Obviously, the accompanying drawings in the following description are only some embodiments of the present application, and for those skilled in the art, other drawings can be obtained from these drawings without creative effort.

[0020] Figure 1 is a flow chart of a preparation method of a perilla seed high-ovophospholipid chicken egg feed of the present application. DETAILED DESCRIPTION

[0021] Embodiments of the present application will be described in detail below, examples of which are shown in the accompanying drawings, wherein the same or similar reference signs represent the same or similar elements or elements having the same or similar functions throughout. The embodiments described below by referring to the accompanying drawings are exemplary and are intended to explain the present application, and cannot be understood as a limitation of the present application.

[0022] The present application provides a perilla seed high-ovophospholipid chicken egg feed, comprising a basic feed and a functional additive, the functional additive is composed of the following components in parts by weight: perilla seed 50-100 parts, choline source 0.1-10 parts, vitamin E 0.05-0.1 parts, bacillus subtilis bacterial liquid 1x10 9 CFU / kg; the basic feed is composed of the following components in parts by weight: corn 60-65 parts, soybean meal 20-25 parts, bran 5-8 parts, stone powder 3-5 parts, calcium hydrogen phosphate 1-2 parts, salt 0.3-0.5 parts, egg chicken premix 1-2 parts.

[0023] The vitamin E is DL-alpha-tocopherol acetate, and the viable bacterial count of the bacillus subtilis is greater than or equal to 2x10 10 CFU / g.

[0024] The choline source is selected from one or more of choline chloride, egg yolk powder, and betaine.

[0025] Please refer to Figure 1 The present application provides a preparation method of a perilla seed high-ovophospholipid chicken egg feed, comprising the following steps: S101 After the perilla seeds are removed, the perilla seeds are crushed to 50-100 mesh by using a super micro grinder, bacillus subtilis bacterial liquid is added, and fermentation is carried out at 30-35℃ for 24-48 hours; after the fermentation is completed, the perilla seeds are dried at 60-70℃ to a moisture content of less than or equal to 12%, to obtain perilla seed powder; S102 The perilla seed powder, choline source, vitamin E, and bacillus subtilis are mixed uniformly in proportion to obtain a functional compound; S103 proportionally put corn, soybean meal, bran, stone powder, dicalcium phosphate, salt and egg chicken premix into a mixer, mix for 10-15 minutes, the variation coefficient of mixing uniformity is ≤7%, to obtain a basic mixed feed; S104 add the functional compound to the basic mixed feed, mix at a speed of 20-25 rpm for 5-10 minutes, then high-speed mix at a speed of 60-70 rpm for 10-15 minutes, finally mix at a speed of 20-25 rpm for 5-8 minutes, the variation coefficient of mixing uniformity is ≤5%, granulate at 80-85℃ by a granulator, cool to room temperature and then package, to obtain the finished feed. The solid-liquid ratio of the fermentation process is 1:0.8 (g / mL) at 30-35℃ for 24-48 hours, the concentration of bacillus subtilis liquid is 1×10 8 CFU / mL, stir every 8 hours during the fermentation, and then vacuum freeze-dry after the fermentation, the drying conditions are: cold trap temperature -50℃, vacuum degree ≤10 Pa, and drying time 12-18 hours.

[0026] Before granulating at 80-85℃ by the granulator, spray 0.1-0.3 wt% of a natural antioxidant solution into the mixed materials, the natural antioxidant is selected from one or more of rosemary extract, tea polyphenol or bamboo leaf flavone.

[0027] After granulating at 80-85℃ by the granulator, the pellet feed is treated by surface coating, the coating material is food-grade chitosan-sodium alginate composite film, and the coating thickness is 20-50 μm.

[0028] Before adding the functional compound into the basic mixed feed, pre-mix with the carrier diatomite at a mass ratio of 1:0.5-1, to improve the dispersion uniformity of the trace ingredients.

[0029] The application provides an application of perilla seed high-ovophospholipid chicken egg feed, the high-ovophospholipid chicken egg feed is used for daily feeding of 180-300 day-old laying hens, the daily feeding amount is 110-120 g per hen, free water is provided, and the feeding is continuously performed for 30-60 days, and the ovophospholipid content in egg yolk is stably 12-15.1 g / 100 g.

[0030] In order to illustrate the beneficial effects of the above scheme, the application sets up five groups of examples to compare with conventional feed.

[0031] Example 1 1.1 Formula composition Functional additives (by weight): perilla seed 80 parts, choline chloride (75% content) 5 parts, DL-α-tocopherol acetate (vitamin E) 0.07 parts, bacillus subtilis liquid (1×10 8 CFU / mL) is added at a solid-liquid ratio of 1:0.8 Basic feed (by weight): 62.5 parts corn, 22.5 parts soybean meal, 6.5 parts wheat bran, 4.0 parts limestone, 1.5 parts dicalcium phosphate, 0.4 parts salt, and 1.5 parts laying hen premix. 1.2 Preparation method After removing impurities, perilla seeds were pulverized to 80 mesh using an ultrafine pulverizer. Bacillus subtilis culture was added at a solid-liquid ratio of 1:0.8, and fermented at 33℃ for 36 hours, stirring every 8 hours. After fermentation, the seeds were freeze-dried under vacuum (cold trap −50℃, vacuum degree ≤10 Pa, 14 hours) to obtain perilla seed powder. The perilla seed powder, choline chloride, and vitamin E were mixed, and then premixed with diatomaceous earth (1:0.8) to obtain a functional compound. The components of the basic feed were mixed for 12 minutes (coefficient of variation ≤7%). The functional compound was added to the basic feed and mixed using a three-stage mixing process (low speed 5 min → high speed 12 min → low speed 6 min), with a final coefficient of variation ≤5%. A 0.2 wt% tea polyphenol aqueous solution was sprayed in, and granulation was performed at 80–85℃. The surface was coated with a chitosan-sodium alginate composite film (35 μm thick) and then cooled and packaged.

[0032] 1.3 Animal Experiment Design Experimental animals: 200 Hy-Line Brown chickens aged 180 days were randomly divided into two groups (control group vs. implementation group), with 100 chickens in each group; Feeding cycle: 60 days; Feeding amount: 115 g / animal / day, free access to water; Control group feed: Commercially available complete feed for laying hens (lecithin enhancement rate ≤10%); Testing indicators: egg yolk lecithin content (g / 100g), egg production rate, egg weight, cholesterol, and heavy metal residues.

[0033] The determination of lecithin content in egg yolks was based on GB 5009.272–2016, "National Food Safety Standard - Determination of Phospholipids in Foods", and the detection method was high performance liquid chromatography-evaporative light scattering detection (HPLC-ELSD).

[0034] The instruments and equipment used are as follows: Liquid Chromatograph: Agilent 1260 Infinity II (Agilent Technologies, USA) Column: ZORBAX SB–C18 (4.6 mm × 250 mm, 5 μm) ELSD detector: Alltech 3300 (drift tube temperature: 50℃, nitrogen flow rate: 1.8 L / min) Mobile phase: acetonitrile:isopropanol:water = 50:45:5 (v / v / v), isocratic elution Flow rate: 1.0 mL / min; injection volume: 20 μL; column temperature: 35℃.

[0035] Sample pretreatment: Take 10 g of fresh egg yolk and add 20 mL of chloroform-methanol (2:1, v / v) to homogenize; Ultrasonic extraction for 30 min (power 200 W, frequency 40 kHz); Centrifuge (8000 rpm, 10 min, 4℃) and collect the lower organic phase; Concentrate to dryness by nitrogen blowing, redissolve with 1 mL of mobile phase, filter through a 0.22 μm filter membrane, and then inject the sample.

[0036] Quantification method: External standard method, using lecithin standard (Sigma-Aldrich, ≥99%) to plot a standard curve (R). 2 >0.999) Cholesterol was determined according to GB 5009.128–2016 "Determination of Cholesterol in Food" - Method I (GC Method). The equipment is a Thermo Scientific TRACE 1310 gas chromatograph with an FID detector. Column: DB-5MS (30 m × 0.25 mm × 0.25 μm) Temperature program: Initial 100℃ (1 min) → 20℃ / min → 280℃ (10 min) Internal standard: 5α-cholestane (used for recovery calibration) The methods for heavy metal detection are based on GB 5009.12–2017 (lead) and GB 5009.15–2014 (cadmium). Equipment: PerkinElmer PinAAcle 900T Atomic Absorption Spectrometer (Graphite Furnace Mode) Sample digestion: Microwave digestion (Milestone ETHOS UP), HNO3 + H2O2 (9:1), 180℃ / 20 min Limits of detection: Pb 0.001 mg / kg, Cd 0.0005 mg / kg 1.4 Experimental Results Table 1 Indicator Control group (regular feed) Example 1 group Lifting range Egg yolk lecithin (g / 100g) 9.8 ± 0.3 13.2 ± 0.4 +34.7% Egg production rate (%) 91.2 ± 1.1 92.5 ± 0.9 No significant difference Average egg weight (g) 62.1 ± 1.2 62.4 ± 1.0 — Cholesterol (mg / 100g egg yolk) 1250 ± 30 1130 ± 25 −9.6% Lead (mg / kg) <0.01 <0.01 In line with national standards Cadmium (mg / kg) <0.01 <0.01 In line with national standards Example 2 2.1 Formula Adjustment Perilla seeds: 85 parts Choline source: 6 parts betaine (98% purity) (to replace choline chloride) The rest is the same as in Example 1. 2.2 Experimental Objective The synergistic effect of different choline sources on the lecithin synthesis pathway was verified, and the synergistic effect was measured using the Bliss independent model calculation method.

[0037] Theoretical additive effect: E_theoretical = E_A + E_B − (E_A × E_B) Where E_A = the enhancement rate of perilla seed alone (20%), and E_B = the enhancement rate of choline source alone (such as betaine, which is 9%). Then E_theoretical = 20% + 9% − (0.2×0.09) = 27.2% Actual observed improvement rate: (13.6 − 9.8) / 9.8 ≈ 38.8% Synergy Index (CI) = E_actual / E_theoretical = 38.8% / 27.2% ≈ 1.43 > 1, indicating the existence of significant positive synergy.

[0038] The choline speciation analysis method was ion chromatography (IC) using a Dionex ICS-5000+ (Thermo Fisher Scientific) instrument, an IonPac CS17 column (4 × 250 mm), methanesulfonic acid (20 mM) as the eluent, and a flow rate of 1.0 mL / min. 2.3 Experimental Results Table 2 Choline source type Egg yolk lecithin (g / 100g) Synergistic effect (Bliss model) No choline source (only perilla seed) 11.8 Benchmark Choline chloride (5 parts) 13.2 Actual increase 45.9%> theory 28% Betaine (6 parts) 13.6 Actual increase 48.2%> theory 29% Egg yolk powder (8 parts) 12.9 Actual increase 42.1% Note: Betaine, due to its methyl donor function, is more effective in promoting the synthesis of phosphatidylcholine, and its synergistic effect is slightly better than that of choline chloride.

[0039] Example 3 3.1 Process Variables Fermentation temperatures: 30℃, 33℃, 35℃ Fermentation time: 24h, 36h, 48h Use 80 portions of perilla seeds, keeping all other conditions the same.

[0040] 3.2 Results Analysis Fermentation conditions Lecithin (g / 100g) Perilla seed bioavailability increase 30℃ / 24h 12.1 +32% 33℃ / 36h 13.5 +43% <- best 35℃ / 48h 12.8 +38% (part of the bacteria activity decreased) Table 3 Conclusion: 33℃ / 36h is the optimal fermentation parameter, which balances enzyme activity and metabolite accumulation.

[0041] Example 4 4.1 Add a solution Control group: No antioxidants Experimental group A: 0.2% rosemary extract Experimental group B: 0.2% tea polyphenols Experimental group C: 0.15% bamboo leaf flavonoids + 0.05% tea polyphenols 4.2 Storage stability test (feeding after 30 days of storage) Group Lecithin (g / 100 g) Vitamin E retention (%) Feed acid value (mgKOH / g) No antioxidant 11.968% 8.2 Rosemary extract 13.492% 4.1 Tea polyphenol 13.390% 4.3 Compound antioxidant 13.594% 3.8 Conclusion: Natural antioxidants significantly delay feed oxidation, protect functional ingredient activity, and the use of compound is recommended.

[0042] Example 5: Optimization of the ratio of functional compound premix carrier 5.1 Premix ratio design Functional compound : diatomite = 1:0.5, 1:0.8, 1:1.0, 1:1.2 5.2 Mixing uniformity and lecithin effect Table 4 Premix ratio Mixing coefficient of variation (%) Lecithin (g / 100g) Batch-to-batch RSD (%) 1:0.5 5.8 12.9 3.2 1:0.8 4.3 13.5 1.8 <- best 1:1.0 4.1 13.4 1.9 1:1.2 3.9 13.3 2.0 (slightly higher cost) Conclusion: The ratio of 1:0.8 achieves the best balance between uniformity, effect and cost.

[0043] The five example core data are summarized as follows Table 5 Example key variables lecithin (g / 100 g) Synergistic lift rate cost change recommendation degree 1 base formula 13.2 + 34.7% benchmark ★★★★★ 2 betaine replacement 13.6 + 48.2% + 3% ★★★★☆ 3 fermentation optimization 13.5 + 46.5% no increase ★★★★★ 4 antioxidant 13.5 + 46.5% + 2% ★★★★☆ 5 premix ratio 13.5 + 46.5% - 1% (reduce waste) ★★★★★ The application realizes the stable improvement of lecithin by more than 45% through the four synergies of "perilla seed + choline source + probiotics + process control"; all examples meet the safety standard of GB 2749-2015, have no negative impact on egg production performance; the process is fully compatible with the existing feed production line, does not need to add new equipment, and has the condition of large-scale promotion; the cost is 15-20% lower than that of the same high lecithin feed on the market, and the economy is outstanding.

[0044] The above only discloses a preferred embodiment of the application, of course, cannot limit the scope of the right of the application, those skilled in the art can understand that all or part of the processes of the above-mentioned embodiments are implemented, and equivalent changes made according to the claims of the application still belong to the scope covered by the application.

Claims

1. A perilla seed high-ovoplasmic egg feed, characterized by, The functional additive comprises perilla seed 50-100 parts by weight, choline source 0.1-10 parts by weight, vitamin E 0.05-0.1 parts by weight, and bacillus subtilis bacterial liquid 1x10 9 CFU / kg. The basic feed is composed of the following components by weight: corn 60-65 parts, soybean meal 20-25 parts, bran 5-8 parts, stone powder 3-5 parts, calcium hydrogen phosphate 1-2 parts, salt 0.3-0.5 parts, and egg hen premix 1-2 parts.

2. The perilla seed high-ovophospholipid chicken egg feed according to claim 1, wherein, The vitamin E is DL-alpha-tocopheryl acetate, the viable bacterial count of the Bacillus subtilis is ≥2×10 10 CFU / g.

3. The perilla seed high-ovophospholipid chicken egg feed according to claim 2, wherein, The choline source is selected from one or more of choline chloride, egg yolk powder, and betaine.

4. A preparation method of perilla seed high-ovophosphatidic chicken egg feed, applied to the perilla seed high-ovophosphatidic chicken egg feed of any one of claims 1-3, characterized in that, The method comprises the following steps: After the perilla seeds are removed of impurities, the perilla seeds are crushed to 50-100 mesh using a super micro grinder, Bacillus subtilis liquid is added, and fermentation is carried out at 30-35°C for 24-48 hours. After the fermentation is completed, the perilla seeds are dried at 60-70°C until the water content is ≤12%, to obtain perilla seed powder; The perilla seed powder, choline source, vitamin E, and Bacillus subtilis are mixed in a proportion to obtain a functional compound; The corn, soybean meal, bran, stone powder, calcium hydrogen phosphate, salt, and egg hen premix are put into a mixer in a proportion, mixed for 10-15 minutes, and the uniformity coefficient of the mixture is ≤7%, to obtain a basic mixed feed; The functional compound is added to the basic mixed feed, mixed at a speed of 20-25 rpm for 5-10 minutes, high-speed mixed at a speed of 60-70 rpm for 10-15 minutes, and finally mixed at a speed of 20-25 rpm for 5-8 minutes, and the uniformity coefficient of the mixture is ≤5%. The mixture is granulated by a granulator at 80-85°C, cooled to room temperature, and packaged, to obtain the finished feed.

5. The perilla seed high-ovophospholipid chicken egg feed and the preparation method thereof according to claim 5, wherein, The solid-liquid ratio of the fermentation process at 30-35℃ for 24-48 hours is 1:0.8 (g / mL), the concentration of Bacillus subtilis bacterial solution is 1×10 8 CFU / mL, stirring every 8 hours during fermentation, vacuum freeze-drying after fermentation, drying conditions: cold trap temperature -50℃, vacuum degree ≤10 Pa, drying time 12-18 hours.

6. The perilla seed high-ovophospholipid chicken egg feed and the preparation method thereof according to claim 6, wherein, Before the mixture is granulated by the granulator at 80-85°C, 0.1-0.3 wt% of a natural antioxidant solution is sprayed into the mixture, and the natural antioxidant is selected from one or more of rosemary extract, tea polyphenol, or bamboo leaf flavone.

7. The perilla seed high-ovophospholipid chicken egg feed and the preparation method thereof according to claim 7, wherein, After the granulation by the granulator at 80-85°C, the pellet feed is subjected to surface coating treatment, and the coating material is food-grade chitosan-sodium alginate composite film, and the coating thickness is 20-50 μm.

8. The perilla seed high-ovophospholipid chicken egg feed and the preparation method thereof according to claim 8, wherein, Before the functional compound is added to the basic mixed feed, the functional compound is premixed with a carrier diatomite in a mass ratio of 1:0.5-1, to improve the dispersion uniformity of the trace components.

9. Application of a perilla seed high-ovophospholipid chicken egg feed, wherein the perilla seed high-ovophospholipid chicken egg feed according to claim 1 is used. The high-ovophospholipid chicken egg feed is used for daily feeding of 180-300-day-old laying hens, the daily feeding amount is 110-120 g per hen, water is provided freely, and the feeding is continuously carried out for 30-60 days. The ovophospholipid content in the egg yolk of the laying hen is stably maintained at 12-15.1 g / 100 g.

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