Double-phase plant extraction essence with anti-inflammatory and antibacterial effects as well as preparation method and application of double-phase plant extraction essence

Abstract

The invention relates to the technical field of cosmetic formula processes, and particularly discloses a double-phase plant extraction essence with anti-inflammatory and antibacterial effects and a preparation method and application thereof.The double-phase plant extraction essence comprises an essence water phase and an essence oil phase, and the water phase comprises extracts of lucid ganoderma, honeysuckle, purslane, apple peel and the like; the oil phase comprises clove essential oil, lemon eucalyptus essential oil, myristica fragrans essential oil, tea tree essential oil and rose geranium essential oil. According to the bidirectional plant extraction essence disclosed by the invention, polyphenol and carbohydrate components in the water phase and terpenoid and phenol essential oil in the oil phase synergistically exert strong anti-oxidation, anti-inflammatory and antibacterial effects, so that free radicals are effectively removed, damage of oxidative stress to skin is relieved, release of inflammatory factors is inhibited, red and swollen phenomena are quickly relieved, acne fading is promoted, and the effect of preventing and treating acne is achieved. The product has a remarkable antibacterial effect, breeding of pathogenic bacteria can be reduced, generation of acnes is reduced from the source, and the product has a remarkable effect in the aspects of removing acnes and improving acne skin through a'oxidation resistance-inflammation resistance-bacterium resistance 'triple mechanism.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of cosmetic formulation technology, and particularly relates to a dual-phase plant extract essence with anti-inflammatory and antibacterial effects and a preparation method and application thereof. BACKGROUND

[0002] Human facial acne generally refers to chronic inflammation of the hair follicle sebaceous gland, which is mainly caused by excessive sebum secretion, abnormal keratinization of hair follicles and infection of propionibacterium acnes, and is a common skin problem, especially in teenagers and young people. Most of the acne-removing care products on the market can remove acne and have effects, and can only repair and modify the face to a certain extent. However, acne marks are generally accompanied by facial inflammation, and the inflammation caused by acne marks cannot be treated. The single target acne-removing product often cannot simultaneously solve the multi-dimensional needs of antibacterial, anti-inflammatory and skin barrier repair. Therefore, the present application proposes a dual-phase plant extract essence with anti-inflammatory and antibacterial effects and a preparation method and application thereof to solve the problems mentioned in the background. SUMMARY

[0003] "Dual phase" is a unique technology and form symbol of the present application, which refers to the fact that the essence is composed of two liquid phases with different textures and effects. The present application aims to solve the shortcomings of existing products and proposes a dual-phase plant extract essence with anti-inflammatory and antibacterial effects and a preparation method and application thereof.

[0004] In order to achieve the above-mentioned purpose, the present application adopts the following technical scheme: A dual-phase plant extract essence with anti-inflammatory and antibacterial effects and a preparation method and application thereof, comprising an essence water phase and an essence oil phase, the water phase comprising extracts of ganoderma lucidum, honeysuckle, gota, apple peel, etc., and the oil phase comprising clove essential oil, lemon eucalyptus essential oil, nutmeg essential oil, tea tree essential oil and rose geranium essential oil, specifically comprising the following steps: Step one, weigh a certain amount of extracts of ganoderma lucidum, honeysuckle, gota and apple peel, and preheat the prepared raw materials, the preheating temperature is controlled at 40-50 DEG C, and the preheating time is 20-30 min. Preheating helps to improve the solubility of the raw materials. Mix to make a ten-fold concentrated solution. In step one, ganoderma lucidum, honeysuckle, gota and apple peel are soaked in ethanol and heated to extract active ingredients therefrom. After extraction, the active liquid is concentrated by evaporation of solvent or reduced pressure concentration to obtain a concentrated solution; Ganoderma lucidum has antioxidant and anti-aging effects, delays wrinkles and sagging, promotes cell repair, enhances skin barrier function, and has anti-inflammatory and soothing repair effects, relieves redness, sensitive skin and other problems, and has moisturizing function, has strong water locking ability, can inhibit the activity of tyrosinase and reduce the production of melanin; honeysuckle with its "natural anti-inflammatory drug" characteristics, especially suitable for sensitive skin, oily acne skin and anti-aging people, also has many functions such as anti-bacterial acne, antioxidant repair and so on; spilanthes acmella has the synergistic effect of anti-inflammatory, anti-histamine, barrier repair and other ways, and becomes the best natural soothing ingredient, which can inhibit inflammatory factors and relieve redness and stinging, suitable for sensitive skin and acne rosacea care, and promote the repair of damaged skin; apple peel has excellent anti-inflammatory, soothing and barrier repair ability, and has broad application prospects in sensitive skin care, acne, post-sun repair and anti-aging fields, and it is rich in quercetin and procyanidin, which can inhibit inflammation, reduce inflammation such as acne and seborrheic dermatitis, and has a certain inhibitory effect on propionibacterium acnes, reducing the recurrence of acne.

[0005] Step two, take a certain amount of clove essential oil, lemon eucalyptus essential oil, nutmeg essential oil, tea tree essential oil and rose geranium essential oil, mix them together and then dissolve them with DMSO to 10ml; Clove essential oil has strong antibacterial and anti-inflammatory effects, and contains eugenol which has strong bacteriostatic effect, so it is suitable for acne skin and acne care, and also has antioxidant effect, can scavenge free radicals and delay aging; the main active components of lemon eucalyptus essential oil are citronellal and citronellol, which can inhibit COX-2, NF-κB and other inflammatory pathways, and reduce the release of TNF-α, IL-6 and other pro-inflammatory factors; it also has significant inhibitory effect on common pathogenic bacteria such as Staphylococcus aureus, propionibacterium acnes and Candida albicans, and has broad application prospects in the fields of anti-acne, sensitive skin care and scalp health due to its anti-inflammatory, bacteriostatic, soothing and insect repellent effects; nutmeg essential oil has bacteriostatic effect, and the volatile oil in nutmeg has certain inhibitory effect on skin pathogenic bacteria, can regulate sebum secretion and reduce pore blockage, can help improve acne problems, and can relieve skin redness and swelling, suitable for topical application on inflamed acne or mild dermatitis area; tea tree essential oil has strong antibacterial, anti-inflammatory and oil control properties, and has significant inhibitory effect on Staphylococcus aureus, Candida albicans and propionibacterium acnes, which can reduce acne formation and prevent skin infection; rose geranium essential oil is rich in monoterpenol compounds such as citronellol, geraniol and linalool, which have antibacterial, anti-inflammatory soothing, sebum regulation and barrier repair effects, for example, they have inhibitory effect on various bacteria and fungi, and rose geranium essential oil can improve acne problems through multi-target effect, and the fragrance is pleasant.

[0006] Step 3: Slowly pour the 10x concentrated solution obtained in Step 1 into the solution in Step 2. The ratio of the aqueous phase to the oil phase should be 3:1 or 4:1. Use a homogenizer to thoroughly mix the two liquids. The emulsifier can form a protective film at the water-oil interface to prevent the two phases from separating. The homogenizer should be used for 5-10 minutes at a speed of 3000-5000 rpm and a temperature of 20-25℃.

[0007] Step 4: Add a measured amount of emulsifier to the mixture obtained in Step 3, and use ultrasonic emulsification to ensure stable fusion of the two liquids. The emulsifier is polysorbate or sodium dodecyl sulfate. The ultrasonic frequency of the ultrasonic emulsification equipment is between 20-40 kHz, the ultrasonic power is between 50-300 W, the emulsification time is between 5-15 min, and the amount of emulsifier accounts for 2-5% of the total liquid volume.

[0008] An application of a biphasic plant extract with anti-inflammatory and antibacterial effects, wherein the biphasic plant extract lotion prepared by the method is applied to the surface care of human skin.

[0009] Compared with the prior art, the beneficial effects of the present invention are as follows: 1. The aqueous phase of the bidirectional plant extract of this invention is mainly composed of polyphenols and sugars, which have strong free radical scavenging ability, can inhibit lipid peroxidation and repair oxidatively damaged skin barrier, while the oil phase is mainly composed of terpenoids and phenolic essential oils, which have synergistic antioxidant properties, inhibit inflammation-related oxidative stress and regulate sebum oxidation stability, and prevent the formation of acne and acne scars. 2. This invention effectively removes free radicals, reduces oxidative stress damage to the skin, and builds a stable defense environment for acne-prone skin. Anti-inflammatory experiments have confirmed that it can significantly inhibit the release of inflammatory factors, quickly relieve redness and swelling, and accelerate acne resolution. At the same time, antibacterial experiments show that it has a clear inhibitory effect on a variety of pathogenic bacteria, which can reduce the formation of acne from the root.

[0010] In summary, the product of this invention addresses acne problems through a triple synergistic mechanism of "antioxidant-anti-inflammatory-antibacterial," targeting multiple pathways and points, and its significant acne-removing efficacy and comprehensive improvement potential have been scientifically verified. Attached Figure Description

[0011] Figure 1 The cell morphology of each group under a microscope in Example 1 of a biphasic plant extract with anti-inflammatory and antibacterial effects proposed in this invention; Figure 2 This is a schematic diagram of cell viability determination using CCK-8 assay in Example 1 of a biphasic plant extract with anti-inflammatory and antibacterial effects proposed in this invention. Figure 3 This is a schematic diagram of the NO release levels of each formulation in Example 1 of a biphasic plant extract with anti-inflammatory and antibacterial effects proposed in this invention.

[0012] Figure 1 In the diagram, A represents normal cells, B represents the model group, C represents formula 1, D represents formula 2, E represents formula 3, and F represents formula 4. Detailed Implementation

[0013] The technical solutions of the present invention will be clearly and completely described below with reference to the accompanying drawings of the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments.

[0014] Example 1: A method for preparing an aqueous phase with anti-inflammatory and antibacterial effects. The effective components in the plant extracts were tested and found that the polysaccharide content in Ganoderma lucidum extract was 81.2%, the polyphenol content in honeysuckle extract was 46.8%, the polyphenol content in apple peel extract was 73.8%, and the polysaccharide content in purslane extract was 73.2%.

[0015] Weigh out 10mg of Ganoderma lucidum extract, 5mg of honeysuckle extract, and 5mg of apple peel extract. And 5 mg of purslane extract was dissolved in 1 mL to prepare a tenfold concentrated solution of Formula 1; Weigh out 5mg of Ganoderma lucidum extract, 10mg of honeysuckle extract, and 5mg of apple peel extract. And 5 mg of purslane extract was dissolved in 1 mL to prepare a tenfold concentrated solution of Formula 2; Weigh out 5mg of Ganoderma lucidum extract, 5mg of honeysuckle extract, and 10mg of apple peel extract. And 5 mg of purslane extract was dissolved in 1 mL to prepare a tenfold concentrated solution of Formula 3; Weigh out 5mg of Ganoderma lucidum extract, 5mg of honeysuckle extract, and 5mg of apple peel extract. And 10 mg of purslane extract was dissolved in 1 mL to prepare a tenfold concentrated solution of Formula 4.

[0016] Table 1. Formulas of various Chinese herbal ingredients (W / V)

[0017] Efficacy determination: First, a CCK-8 cell viability assay was performed to detect the toxicity of the ingredients in the formulation, ensuring its safety. Secondly, to avoid false positive results in the anti-inflammatory experiment, RAW264.7 macrophages were revived and passaged 2-3 times until their characteristics stabilized. Then, 1× The formulation was seeded at a density of 1000 cells / mL in 96-well plates and incubated for 24 hours. The formulation was diluted 10 times with culture medium, and the culture medium containing four formulations was incubated for another 24 hours. Then, 10 μL of LCK-8 was added to each well and incubated for 1 hour for observation. Based on the CCK-8 experiment, cells were seeded at a density of 3 × 10⁻⁶ cells / mL in 12-well plates and cultured for 24 h. The culture medium was discarded, and the cells were incubated with a medium containing four plant extract formulations for 2 h. Except for the blank control group, 1.5 μL of LPS (1 mg / mL) was added to each group. The cell plates were shaken in a cross shape and cultured at 37°C for 24 h. The cell supernatant was collected, and the NO concentration in the supernatant was determined using a NO kit.

[0018] Results analysis: such as Figure 1 As shown, the RAW264.7 cells in the treatment groups of Formula 1 and Formula 4 exhibited intact morphology, good adhesion, and normal pseudopodia extension. No obvious cell shrinkage or floating was observed, indicating that neither formulation had an adverse effect on cell state. The CCK-8 cell viability test results are as follows: Figure 2 As shown, all four formulations exhibited certain biocompatibility within the tested concentration range. Formulation 1 significantly promoted cell proliferation (with a survival rate of 105%), suggesting that it may have cell protection or metabolic activation effects. like Figure 3 As shown, after LPS stimulation, the NO release in the model group increased significantly, while the NO release was also significantly increased. Figure 1 We can also see that the model group cells are significantly polarized, indicating that the inflammation model was successfully constructed. All formulations can inhibit NO production and have anti-inflammatory activity. Among them, formulations 1 and 4 have the most significant anti-inflammatory activity, while formulations 2 and 3 also show moderate anti-inflammatory activity.

[0019] Example 2: A method for preparing an oil phase with anti-inflammatory and antibacterial effects. 20 μL of clove essential oil, 20 μL of lemon eucalyptus essential oil, 10 μL of nutmeg essential oil, 10 μL of tea tree essential oil, and 10 μL of rose geranium essential oil were dissolved in DMSO and brought to a final volume of 10 mL to obtain solvent formula 5. 10 μL of clove essential oil, 10 μL of lemon eucalyptus essential oil, 5 μL of nutmeg essential oil, 5 μL of tea tree essential oil, and 5 μL of rose geranium essential oil were dissolved in DMSO and brought to a final volume of 10 mL to obtain solvent formula 6. Table 2. Essential oil content (V / V) in the two oil phase formulations.

[0020] The samples were serially diluted using Mueller-Hinton broth (MHB) in a concentration gradient, with each diluted sample (100 μL) being mixed with 50 μL of bacterial suspension (1x). The mixture (CFU / mL) was prepared, where CFU stands for colony-forming units (representing the number of viable bacteria that can grow into visible colonies per milliliter of liquid), and then incubated at 37°C for 16 hours. The MIC was defined as the concentration of the sample at which no bacterial growth could be observed with the naked eye. Take 50 μL of liquid from all wells that did not change color, spread it evenly on hydrolyzed casein peptone agar medium, and incubate it in a 37°C biochemical incubator for 24 h. The lowest sample concentration corresponding to no bacterial growth is the minimum bactericidal concentration.

[0021] Results Analysis: Both Formula 5 and Formula 6 showed good antibacterial effects, with better results against Staphylococcus aureus and Escherichia coli. Specific results for Formula 5 are shown in Table 3, and for Formula 6 in Table 4. Formula 6 contains half the amount of plant essential oil as Formula 5, yet still achieves good antibacterial effects. Therefore, considering factors such as cost, Formula 6 is the better choice.

[0022] Table 3 shows the MIC and MBC in Formula 5.

[0023] Table 4 shows the MIC and MBC in Formula 6.

[0024] In summary: Based on the above analysis of the anti-inflammatory effects in the aqueous phase and the antibacterial effects in the oil phase, firstly, considering the safety and anti-inflammatory activity of the aqueous phase, we can determine that the optimal formulations are Formulation 1, which is mainly composed of Ganoderma lucidum extract, and Formulation 4, which is mainly composed of Portulaca oleracea extract. In the oil phase, considering both antibacterial activity and cost, we recommend Formulation 6, which uses a smaller amount, thus improving the overall formulation. The optimal formulation is shown in Table 5 below: Table 5 Optimal Formulations for Aqueous and Oil Phases

[0025] The above description is only a preferred embodiment of the present invention, but the scope of protection of the present invention is not limited thereto. Any equivalent substitutions or modifications made by those skilled in the art within the scope of the technology disclosed in the present invention, based on the technical solution and inventive concept of the present invention, should be covered within the scope of protection of the present invention.

Claims

1. A method for preparing a biphasic plant extract with anti-inflammatory and antibacterial effects, characterized in that, It includes an aqueous phase and an oil phase. The aqueous phase includes extracts of Ganoderma lucidum, honeysuckle, purslane, and apple peel, while the oil phase includes clove essential oil, lemon eucalyptus essential oil, nutmeg essential oil, tea tree essential oil, and rose geranium essential oil. The specific steps include: Step 1: Weigh out a certain amount of extracts from Ganoderma lucidum, honeysuckle, purslane, and apple peel, and mix them to make a tenfold concentrated solution. Step 2: Take measured amounts of clove essential oil, lemon eucalyptus essential oil, nutmeg essential oil, tea tree essential oil, and rose geranium essential oil, mix them, and then dissolve and bring the volume to 10ml using DMSO. Step 3: Slowly pour the tenfold concentrated solution obtained in Step 1 into the solution in Step 2, and use a homogenizer to thoroughly mix the two liquids. Step four: Add a measured amount of emulsifier to the mixture obtained in step three, and use ultrasonic emulsification to ensure that the two liquids can be stably fused together.

2. The method for preparing the biphasic plant extract with anti-inflammatory and antibacterial effects according to claim 1, characterized in that, In step one, Ganoderma lucidum, honeysuckle, purslane, and apple peel are soaked in ethanol and heated to extract the active ingredients. The extracted active liquid is then concentrated by evaporating the solvent or by reducing the pressure.

3. The method for preparing the biphasic plant extract with anti-inflammatory and antibacterial effects according to claim 1, characterized in that, In step four, the emulsifier is polysorbate or sodium dodecyl sulfate.

4. The method for preparing the biphasic plant extract with anti-inflammatory and antibacterial effects according to claim 1, characterized in that, In step three, the homogenizer is used to stir for 5-10 minutes at a speed of 3000-5000 rpm at a temperature of 20-25℃.

5. The method for preparing the biphasic plant extract with anti-inflammatory and antibacterial effects according to claim 1, characterized in that, In step four, the ultrasonic emulsification equipment used has an ultrasonic frequency of 20-40KHz, an ultrasonic power of 50-300w, an emulsification time of 5-15min, and an emulsifier dosage of 2-5% of the total liquid volume.

6. The method for preparing the biphasic plant extract with anti-inflammatory and antibacterial effects according to claim 1, characterized in that, In step one, the raw materials need to be preheated. The preheating temperature is controlled at 40-50℃ and the preheating time is 20-30 minutes. Preheating helps to improve the solubility of the raw materials.

7. The method for preparing the biphasic plant extract with anti-inflammatory and antibacterial effects according to claim 1, characterized in that, In step three, the ratio of the aqueous phase to the oil phase is 3:1 or 4:

1.

8. An application of a biphasic plant extract with anti-inflammatory and antibacterial effects as described in any one of claims 1-7, characterized in that, The application of the bidirectional plant extract in the preparation of cosmetic products.

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