Plant extract composition with anti-inflammatory and soothing effects and preparation method thereof

Abstract

The invention relates to the technical field of natural plant extracts, and particularly discloses a plant extract composition with anti-inflammatory and soothing effects and a preparation method thereof. The traditional Chinese medicine composition is prepared from the following components in percentage by weight: 1 to 5 percent of radix cynanchi atrati extract, 1 to 3 percent of flos magnoliae extract, 1 to 5 percent of flos hibisci mutabilis extract, 0.5 to 1 percent of herba andrographitis extract, 1 to 3 percent of citrus peel extract, 0.5 to 1 percent of rhizoma paridis extract and the balance of solvent. Six plant extracts with different anti-inflammatory mechanisms are scientifically screened and proportioned, the extraction process is optimized, the plant extracts have a synergistic effect, and the anti-inflammatory effect is improved by adjusting the proportion; the six components respectively act on different links of inflammatory response to form a complementary anti-inflammatory network, the plant extract composition with multiple anti-inflammatory pathways and synergistic interaction is developed, and the problems of single anti-inflammatory effect, insufficient permeability and the like in the prior art are solved.

Description

Technical Field

[0001] This invention relates to the field of natural plant extract technology, specifically to a plant extract composition with anti-inflammatory and soothing effects and its preparation method. Background Technology

[0002] With increasing environmental pollution and rising life stress, skin inflammation and sensitivity are becoming increasingly common. While traditional anti-inflammatory drugs such as corticosteroids are highly effective, long-term use can lead to side effects such as skin atrophy and telangiectasia. Chemically synthesized anti-inflammatory ingredients, on the other hand, pose potential irritation and safety concerns. Therefore, developing naturally derived, highly effective, and safe anti-inflammatory and soothing ingredients has become a current research hotspot. However, the extraction of plant extracts presents numerous challenges: low extraction efficiency, traditional processes often resulting in incomplete dissolution, high purification difficulty, difficulty in separating impurities, and easy loss of active ingredients. In traditional Chinese medicine theory, many plants have been proven to have anti-inflammatory and skin-repairing effects, but how to formulate compound combinations to obtain significantly effective compositions remains a challenge.

[0003] In the prior art, some plant extracts have been used in anti-inflammatory products. For example, patent CN115487112B discloses an anti-inflammatory and soothing composition containing glycyrrhizic acid, bisabolol, citrus peel extract, and Cynanchum atratum extract. Patent CN117338678A discloses the application of Paris polyphylla extract in anti-inflammatory and soothing effects on the skin. However, these technologies mostly use single or small amounts of plant ingredients, failing to fully utilize the synergistic effect of multiple plant active ingredients, and have shortcomings in terms of extract purity and activity, penetration enhancement technology, and stability. Summary of the Invention

[0004] The technical problem to be solved by the present invention is to provide a plant extract composition with anti-inflammatory and soothing effects and its preparation method, so as to solve the problems of low purity and activity of extracts, single anti-inflammatory effect and insufficient permeability in the prior art.

[0005] The present invention solves the above-mentioned technical problems. The present invention provides a plant extract composition with anti-inflammatory and soothing effects, comprising the following components in weight percentage: 1-5% of Cynanchum paniculatum extract, 1-3% of Magnolia officinalis extract, 1-5% of Hibiscus mutabilis flower extract, 0.5-1% of Andrographis paniculata extract, 1-3% of Citrus reticulata peel extract, 0.5-1% of Paris polyphylla extract, and the balance being solvent. The solvent is one or more of water, glycerol, propylene glycol, and butylene glycol; Preparation of the *Cynanchum paniculatum* extract: Dried *Cynanchum paniculatum* rhizomes were pulverized into coarse powder. A 0.05-0.10 mol / L citrate-sodium citrate buffer solution was added at a solid-liquid ratio of 1:8-12 g / mL. Enzymatic hydrolysis was performed for 80-100 min with 0.05-0.1% (by weight of the *Cynanchum paniculatum* root powder) of an enzyme complex. After reflux extraction, the extracts were combined and concentrated under reduced pressure. The resulting extract was then extracted sequentially with petroleum ether and ethyl acetate to obtain the *Cynanchum paniculatum* extract. HPLC analysis showed that the extract contained active substances such as cynanchin. The enzyme complex consists of CaCl2, disodium ethylenediaminetetraacetate, and a compound enzyme in a mass ratio of 3:3:1-3; the compound enzyme consists of cellulase, hemicellulase, and pectinase in a mass ratio of 2-4:2-4:1; it reduces glycoside degradation and impurity content, effectively decomposes fiber, and improves the dissolution rate of active ingredients. The preparation of the Magnolia officinalis extract involves drying Magnolia officinalis buds to a moisture content of 6-8%, pulverizing them to 60 mesh, steam-exploding the Magnolia officinalis powder under a pressure of 0.4-0.6 MPa for 40-50 seconds, adding pure water and extracting under a pressure of 0.1-0.2 MPa for 20-30 minutes, followed by extraction under normal pressure for 40-50 minutes. The extracts are combined, compressed under reduced pressure, and the resulting product is obtained by molecular distillation. This process removes impurities such as waxes and pigments, significantly improving the purity of the active ingredient magnolol and volatile oils, and reducing the volatilization of volatile oils. Preparation of Andrographis paniculata extract: Take dried powder of whole Andrographis paniculata herb, add extractant at a solid-liquid ratio of 1:9-11 g / mL, heat and reflux at 60-70℃ for 40-50 min, extract 2-3 times, combine extracts, compress under reduced pressure to obtain extract, dissolve and filter, extract the filtrate with dichloromethane, and then purify by silica gel column chromatography; Andrographis paniculata extract contains andrographolide monomer with a purity of 98.5%, this method reduces the side extraction of impurities such as chlorophyll; The extract is a citrate-disodium hydrogen phosphate buffer solution containing 5-8% mannitol and 2-3% neutral alumina. Preparation of the citrus peel extract: Fresh citrus peel is freeze-dried at -20~-50℃ and then pulverized to 40-100 mesh. A compound solvent is added at a material-to-liquid ratio of 1:6-8. A compound biological adjuvant is added at 0.05-0.1% of the peel weight. Enzymatic hydrolysis is carried out at 40-45℃ for 80 minutes, followed by extraction at 50℃ for 30 minutes at 300W. This extraction is repeated 2-3 times. The extracts are combined, the solvent is recovered under reduced pressure, and the pH is adjusted to 5.0-5.5. The crude extract is obtained by centrifugation and purified using a macroporous adsorption resin column. Impurities are first removed by elution with 20% ethanol, followed by elution with 50% ethanol. The 50% ethanol eluent is collected, concentrated under reduced pressure, and spray-dried to obtain the citrus peel extract. This improves the solubility and dissolution efficiency of active substances such as hesperidin and methoxyflavonoids, and reduces the oxidation and volatilization of essential oils. The composite solvent is pure water containing citric acid and glucose in a mass ratio of 1-3:10-13; the composite biological adjuvant is rhamnolipid and pectinase in a mass ratio of 4-7:1. Preparation of the Paris polyphylla extract: The dried rhizome of Paris polyphylla was pulverized and extracted three times by reflux with 4-6 times the amount of a eutectic solvent, each time for 2 hours. The extracts were combined and concentrated under reduced pressure. The resulting extract was eluted with a D101 macroporous resin column, sequentially with water, 30% ethanol, and 70% ethanol. The eluent was dried under reduced pressure. The eutectic solvent was lactic acid and glycerol in a volume ratio of 1:1-3. The extract contained Paris polyphylla saponins I, II, and VII. This method inhibits the formation of complexes between saponins and polysaccharides, thus improving the separation rate of saponins. Preparation of the Hibiscus mutabilis flower extract: Fresh Hibiscus mutabilis flowers were added to a mixed solvent of 20%-30% ethanol and 0.02%-0.04% acetic acid at a solid-liquid ratio of 1:5-8 g / mL. The extract was then extracted with ultrasound-assisted extraction at a nitrogen atmosphere, power 200-300W, and temperature 40-50℃ for 20-30 minutes. After microfiltration through a 0.22μm ceramic membrane, the filtrate was concentrated under reduced pressure and then freeze-dried to obtain the extract. This method improves the dissolution content and activity of active ingredients and prevents oxidation.

[0006] Baiwei extract: exerts anti-inflammatory effects by inhibiting the NF-κB signaling pathway and reducing the release of inflammatory mediators such as prostaglandin E2 (PGE2) and leukotrienes. It also has antibacterial and analgesic effects and can relieve skin discomfort.

[0007] Magnolia oil: Reduces vascular permeability in inflamed tissues, inhibits inflammatory exudation and swelling, and has an inhibitory effect on both acute and chronic inflammation. Its volatile components can also promote the transdermal absorption of other active ingredients.

[0008] Andrographolide: Inhibits the production and release of bacterial endotoxins, blocks bacterial drug resistance pathways, and enhances the phagocytic function of leukocytes and macrophages, reducing inflammatory triggers at the source.

[0009] Citrus peel extract: rich in polymethoxyflavonoids (such as noriheptacortin), which have strong antioxidant and anti-inflammatory activities, can neutralize free radicals and reduce inflammatory responses caused by oxidative stress.

[0010] Paris saponins: By inhibiting NF-κB activation, they reduce the expression of pro-inflammatory cytokines such as IL-1, IL-6, and IL-8, while stimulating glucocorticoid secretion and enhancing macrophage function.

[0011] Hibiscus mutabilis flowers contain flavonoids and anthocyanins, which have a significant inhibitory effect on hemolytic streptococci and Staphylococcus aureus.

[0012] The beneficial effects of this invention are as follows: the synergistic effect among the six plant extracts in the plant extract composition provided by this invention enhances the anti-inflammatory effect through ratio adjustment; the six components act on different stages of the inflammatory response, forming a complementary anti-inflammatory network; Baiwei and Paris polyphylla mainly inhibit the NF-κB pathway; Magnolia officinalis controls vascular response; Andrographis paniculata targets pathogens; citrus peel neutralizes free radicals, achieving comprehensive anti-inflammatory effects; the volatile components in Magnolia officinalis oil can act as natural penetration enhancers, helping other macromolecular active ingredients (such as Paris polyphylla saponins) to penetrate the skin barrier; the polyphenols in citrus peel have antioxidant effects, which can protect the stability of other easily oxidized components (such as andrographolide) and extend the product's shelf life; Paris polyphylla extract may have some irritation, but the soothing properties of Baiwei and Magnolia officinalis can neutralize this irritation, making the composition gentler and suitable for sensitive skin; The plant extract extraction methods of this invention are green and efficient. Specifically, the extraction of *Cynanchum paniculatum* utilizes a synergistic enzyme complex to disrupt cell walls, employs CaCl2 to enhance enzyme activity, and uses EDTA-2Na chelation to prevent degradation. Enzymatic hydrolysis via a complex enzyme breaks down the cell walls, avoiding hydrolysis of active ingredients. The extraction of *Magnolia denudata* employs a low-pressure, low-temperature process, using a stepwise process of steam explosion and extraction pressure to avoid oxidation of essential oils and degradation of magnolol caused by high temperatures. The extraction of *Andrographis paniculata* utilizes a dual-function extractant: mannitol (protecting andrographolide from degradation) and neutral alumina (simultaneously adsorbing chlorophyll and removing impurities) to improve extract purity. The extraction of citrus fruits uses citric acid-glucose water combined with rhamnolipids to improve hesperidin solubility while avoiding organic solvent residue. The extraction of *Paris polyphylla* utilizes a low-melting-point solvent for dissociation, specifically disrupting the hydrogen bonds between polysaccharides and saponins using a lactic acid-glycerol low-melting-point solvent, thus increasing saponin dissolution and solving the problem of difficult complex dissociation in traditional processes. Attached Figure Description

[0013] Figure 1 Microscopic image of cell scratch repair in Example 1; Figure 2 Chromatographic results of the extract of *Cynanchum paniculatum* prepared in Example 1 and Comparative Example 1 are shown. Figure 3The chromatograms of andrographolide prepared in Example 1 and Comparative Example 4 are shown. Figure 4 The chromatograms are those of the Paris polyphylla extract prepared in Example 1 and Comparative Example 3. Detailed Implementation

[0014] The present invention will be further described in detail below through specific implementation examples. It should be understood that these embodiments are only used to illustrate the present invention and are not intended to limit the scope of protection of the present invention. After reading the present invention, any modifications of the present invention in various equivalent forms by those skilled in the art fall within the scope of the appended claims.

[0015] Unless otherwise specified, all raw materials and reagents used in this invention are from the conventional market.

[0016] Example 1 A plant extract composition with anti-inflammatory and soothing effects comprises the following components in weight percentage: 3% Cynanchum paniculatum extract, 2% Magnolia officinalis extract, 3% Hibiscus mutabilis flower extract, 0.8% Andrographis paniculata extract, 2% Citrus reticulata peel extract, 0.8% Paris polyphylla extract, with the balance being water; Preparation of the extract of Cynanchum atratum: The dried rhizome of Cynanchum atratum was crushed into coarse powder, and 0.08 mol / L citrate-sodium citrate buffer was added at a solid-liquid ratio of 1:10 g / mL. 0.08% (by weight of Cynanchum atratum root powder) of enzyme complex was added for enzymatic hydrolysis for 90 min. After reflux extraction, the extracts were combined and concentrated under reduced pressure. The resulting extract was then extracted with petroleum ether and ethyl acetate in sequence. The enzyme complex consists of CaCl2, disodium ethylenediaminetetraacetate, and a compound enzyme in a mass ratio of 3:3:2; the compound enzyme consists of cellulase, hemicellulase, and pectinase in a mass ratio of 3:3:1. The preparation of the Magnolia officinalis extract is as follows: Magnolia officinalis buds are dried to a moisture content of 7%, pulverized to 60 mesh, the Magnolia officinalis powder is steam-exploded at a pressure of 0.5 MPa for 45 seconds, pure water is added and extracted at a pressure of 0.1 MPa for 25 minutes, then extracted at normal pressure for 45 minutes, the extracts are combined, compressed under reduced pressure, and the resulting product is obtained by molecular distillation. Preparation of the Andrographis paniculata extract: Take the dried powder of the whole Andrographis paniculata herb, add the extract solution at a solid-liquid ratio of 1:10 g / mL, heat and reflux at 65℃ for 45 min, extract twice, combine the extracts, compress under reduced pressure to obtain the extract, dissolve and filter, extract the filtrate with dichloromethane, and then purify by silica gel column chromatography; the extract solution is a citrate-disodium hydrogen phosphate buffer solution containing 6% mannitol and 2% neutral alumina. Preparation of the citrus peel extract: Fresh citrus peel was freeze-dried at -30℃ and then pulverized to 40-100 mesh. A compound solvent was added at a material-to-liquid ratio of 1:7. A compound biological adjuvant was added at 0.08% of the peel weight. The mixture was enzymatically hydrolyzed at 40℃ for 80 minutes, and then extracted at 50℃ for 30 minutes at 300W. The extraction was repeated twice. The extracts were combined, the solvent was recovered under reduced pressure, the pH was adjusted to 5.0, and the crude extract was obtained by centrifugation. The crude extract was purified by macroporous adsorption resin column purification. First, 20% ethanol was used to remove impurities, and then 50% ethanol was used to elute. The 50% ethanol eluent was collected, concentrated under reduced pressure, and spray-dried to obtain the extract. The composite solvent is pure water containing citric acid and glucose in a mass ratio of 2:12; the composite biological adjuvant is rhamnolipid and pectinase in a mass ratio of 6:1. Preparation of the Paris polyphylla extract: Take the dried rhizome of Paris polyphylla, pulverize it, add 5 times the amount of eutectic solvent and reflux extract three times, 2 hours each time, combine the extracts, concentrate under reduced pressure, and elute the obtained extract with a D101 macroporous resin column, successively with water, 30% ethanol and 70% ethanol, and dry the eluent under reduced pressure; the eutectic solvent is lactic acid and glycerol in a volume ratio of 1:2; Preparation of the Hibiscus mutabilis flower extract: Fresh Hibiscus mutabilis flowers were added to a mixed solvent of 25% ethanol and 0.03% acetic acid at a solid-liquid ratio of 1:7 g / mL. The extract was ultrasonically assisted for 25 min under a nitrogen atmosphere at a power of 250 W and a temperature of 45 °C. After microfiltration through a 0.22 μm ceramic membrane, the filtrate was concentrated under reduced pressure and then freeze-dried to obtain the extract.

[0017] The plant extract composition prepared in the examples was subjected to a repair effect test (cell scratch repair). The cell scratch assay is a laboratory technique that studies cell migration, repair capacity, and intercellular interactions by scratching a cell monolayer and periodically capturing images using a time-lapse microscope. The basic principle is: an artificial blank area, called a "scratch / wound," is created on the fused monolayer of cells. Cells at the edge of the scratch gradually enter the blank area, causing the "scratch / wound" to heal. Images are captured at the beginning and periodically during cell migration. By measuring the scratch distance at different time points and calculating the difference, the cell migration capacity can be assessed. In this invention, a blank group was set up as a control group. The final test results are as follows: Figure 1 As shown, the plant extract composition prepared by the present invention has a good repair effect. Within the cell scratch 23, compared with the blank group, the cell migration rate of Example 1 at a concentration of 2.5% was significantly improved (P<0.05), indicating that the sample has a significant repair effect on 3T3 cells, and the repair effect is more obvious compared with the control group.

[0018] Example 2 A plant extract composition with anti-inflammatory and soothing effects, comprising the following components by weight percentage: 2% Cynanchum paniculatum extract, 3% Magnolia officinalis extract, 1% Hibiscus mutabilis flower extract, 1% Andrographis paniculata extract, 1% Citrus reticulata peel extract, 1% Paris polyphylla extract, with the balance being glycerin; Preparation of the extract of Cynanchum atratum: The dried rhizome of Cynanchum atratum was crushed into coarse powder, and 0.05 mol / L citrate-sodium citrate buffer was added at a solid-liquid ratio of 1:8 g / mL. 0.05% (by weight of Cynanchum atratum root powder) of enzyme complex was added for enzymatic hydrolysis for 80 min. After reflux extraction, the extracts were combined and concentrated under reduced pressure. The resulting extract was then extracted with petroleum ether and ethyl acetate in sequence. The enzyme complex consists of CaCl2, disodium ethylenediaminetetraacetate, and a compound enzyme in a mass ratio of 3:3:1; the compound enzyme consists of cellulase, hemicellulase, and pectinase in a mass ratio of 2:2:1. The preparation of the Magnolia officinalis extract is as follows: Magnolia officinalis buds are dried to a moisture content of 6%, pulverized to 60 mesh, the Magnolia officinalis powder is steam-exploded at a pressure of 0.4 MPa for 40 seconds, pure water is added and extracted at a pressure of 0.2 MPa for 20 minutes, then extracted at normal pressure for 40 minutes, the extracts are combined, compressed under reduced pressure, and the resulting product is obtained by molecular distillation. Preparation of the Andrographis paniculata extract: Take the dried powder of the whole Andrographis paniculata herb, add the extract solution at a solid-liquid ratio of 1:9 g / mL, heat and reflux at 60℃ for 40 min, extract twice, combine the extracts, compress under reduced pressure to obtain the extract, dissolve and filter, extract the filtrate with dichloromethane, and then purify by silica gel column chromatography; the extract solution is a citrate-disodium hydrogen phosphate buffer solution containing 5% mannitol and 2% neutral alumina. Preparation of the citrus peel extract: Fresh citrus peel was freeze-dried at -20℃ and then pulverized to 40-100 mesh. A compound solvent was added at a material-to-liquid ratio of 1:6. A compound biological adjuvant was added at 0.05% of the peel weight. The mixture was enzymatically hydrolyzed at 40℃ for 80 minutes, and then extracted at 50℃ for 30 minutes at 300W. The extraction was repeated twice. The extracts were combined, the solvent was recovered under reduced pressure, the pH was adjusted to 5.0, and the crude extract was obtained by centrifugation. The crude extract was purified by macroporous adsorption resin column purification. First, 20% ethanol was used to remove impurities, and then 50% ethanol was used to elute. The 50% ethanol eluent was collected, concentrated under reduced pressure, and spray-dried to obtain the extract. The composite solvent is pure water containing citric acid and glucose in a mass ratio of 1:10; the composite biological adjuvant is rhamnolipid and pectinase in a mass ratio of 4:1. Preparation of the Paris polyphylla extract: Take the dried rhizome of Paris polyphylla, pulverize it, add 4-6 times the amount of eutectic solvent and reflux extract three times, 2 hours each time. Combine the extracts, concentrate under reduced pressure, and elute the obtained extract with a D101 macroporous resin column, successively with water, 30% ethanol, and 70% ethanol. Dry the eluent under reduced pressure. The eutectic solvent is lactic acid and glycerol in a volume ratio of 1:1. Preparation of the Hibiscus mutabilis flower extract: Fresh Hibiscus mutabilis flowers were added to a mixed solvent of 20% ethanol and 0.02% acetic acid at a solid-liquid ratio of 1:5 g / mL. The extract was extracted with ultrasonic assistance at a power of 200W and a temperature of 40℃ under a nitrogen atmosphere for 20 min. After microfiltration through a 0.22μm ceramic membrane, the filtrate was concentrated under reduced pressure and then freeze-dried to obtain the extract.

[0019] Example 3 A plant extract composition with anti-inflammatory and soothing effects comprises the following components by weight percentage: 5% Cynanchum paniculatum extract, 3% Magnolia officinalis extract, 5% Hibiscus mutabilis flower extract, 1% Andrographis paniculata extract, 3% Citrus reticulata peel extract, 1% Paris polyphylla extract, and the balance being propylene glycol. Preparation of the extract of Cynanchum atratum: Take dried Cynanchum atratum rhizome and crush it into coarse powder. Add 0.10 mol / L citrate-sodium citrate buffer solution at a solid-liquid ratio of 1:12 g / mL. Add 0.1% (by weight of Cynanchum atratum root powder) of enzyme complex for 100 min of enzymatic hydrolysis. After reflux extraction, combine the extracts and concentrate under reduced pressure. Extract the obtained extract with petroleum ether and ethyl acetate in sequence. The enzyme complex consists of CaCl2, disodium ethylenediaminetetraacetate, and a compound enzyme in a mass ratio of 3:3:3; the compound enzyme consists of cellulase, hemicellulase, and pectinase in a mass ratio of 4:4:1. The preparation of the Magnolia officinalis extract is as follows: Magnolia officinalis buds are dried to a moisture content of 8%, pulverized to 60 mesh, the Magnolia officinalis powder is steam-exploded at a pressure of 0.6 MPa for 40-50 seconds, pure water is added and extracted at a pressure of 0.2 MPa for 30 minutes, then extracted at normal pressure for 50 minutes, the extracts are combined, compressed under reduced pressure, and the resulting product is obtained by molecular distillation. Preparation of the Andrographis paniculata extract: Take the dried powder of the whole Andrographis paniculata herb, add the extract solution at a solid-liquid ratio of 1:11 g / mL, heat and reflux at 70℃ for 50 min, extract three times, combine the extracts, compress under reduced pressure to obtain the extract, dissolve and filter, extract the filtrate with dichloromethane, and then purify by silica gel column chromatography; the extract solution is a citrate-disodium hydrogen phosphate buffer solution containing 8% mannitol and 3% neutral alumina. Preparation of the citrus peel extract: Fresh citrus peel was freeze-dried at -50℃ and then pulverized to 40-100 mesh. A compound solvent was added at a material-to-liquid ratio of 1:8. A compound biological adjuvant was added at 0.1% of the peel weight. Enzymatic hydrolysis was carried out at 45℃ for 80 minutes. Then, extraction was performed at 50℃ for 30 minutes with a power of 300W. The extraction was repeated 3 times. The extracts were combined, the solvent was recovered under reduced pressure, the pH was adjusted to 5.5, and the crude extract was obtained by centrifugation. The crude extract was purified by macroporous adsorption resin column purification. First, 20% ethanol was used to remove impurities, and then 50% ethanol was used to elute. The 50% ethanol eluent was collected, concentrated under reduced pressure, and spray-dried to obtain the extract. The composite solvent is pure water containing citric acid and glucose in a mass ratio of 3:13; the composite biological adjuvant is rhamnolipid and pectinase in a mass ratio of 7:1. Preparation of the Paris polyphylla extract: Take the dried rhizome of Paris polyphylla, pulverize it, add 6 times the amount of eutectic solvent and reflux extract three times, 2 hours each time, combine the extracts, concentrate under reduced pressure, and elute the obtained extract with a D101 macroporous resin column successively with water, 30% ethanol and 70% ethanol, and dry the eluent under reduced pressure; the eutectic solvent is lactic acid and glycerol in a volume ratio of 1:3; Preparation of the Hibiscus mutabilis flower extract: Fresh Hibiscus mutabilis flowers were added to a mixed solvent of 30% ethanol and 0.04% acetic acid at a solid-liquid ratio of 1:8 g / mL. The extract was ultrasonically assisted for 30 min under a nitrogen atmosphere at a power of 300 W and a temperature of 50 °C. After microfiltration through a 0.22 μm ceramic membrane, the filtrate was concentrated under reduced pressure and then freeze-dried to obtain the extract.

[0020] Comparative Example 1 The difference between Comparative Example 1 and Example 1 is that Comparative Example 1 uses commercially available Cynanchum alopecuroides extract instead of the prepared Cynanchum alopecuroides extract, while everything else remains the same.

[0021] Comparative Example 2 The difference between Comparative Example 2 and Example 1 is that Comparative Example 2 does not contain Magnolia officinalis extract, while everything else remains the same.

[0022] Comparative Example 3 The difference between Comparative Example 3 and Example 1 is that Comparative Example 3 uses commercially available Paris polyphylla extract instead of the prepared Paris polyphylla extract, while everything else remains the same.

[0023] Comparative Example 4 The difference between Comparative Example 4 and Example 1 is that Comparative Example 4 uses commercially available Andrographis paniculata extract instead of the prepared Andrographis paniculata extract, while everything else remains the same.

[0024] Comparative Example 5 The difference between Comparative Example 5 and Example 1 is that Comparative Example 5 does not contain citrus peel extract, while everything else remains the same.

[0025] Comparative Example 6 The difference between Comparative Example 6 and Example 1 is that Comparative Example 6 does not contain Hibiscus mutabilis flower extract, while everything else remains the same.

[0026] The extracts of Cynanchum paniculatum, Paris polyphylla, and Andrographis paniculata prepared in Example 1 were subjected to gas chromatography analysis with those of Comparative Examples 1 and 3-4. The results are as follows: Figure 2-4 As shown, the extract prepared by the method of the present invention has a high content of effective components and few impurities.

[0027] Experiment 1: Effect of xylene on mouse ear swelling Fifty healthy Kunming mice were randomly divided into five groups: a model control group, a positive control group (dexamethasone), and high, medium, and low dose groups of the plant extract composition prepared in Example 1 of this invention (equivalent to final concentrations of 5%, 3%, and 1%, respectively). Each group of mice had 30 μL of xylene applied to both sides of their right ear to induce inflammation, with the left ear serving as a control. One hour before inflammation induction, the positive control group was administered dexamethasone 5 mg / kg by gavage, and each dose group of the composition of this invention had 0.1 mL of the corresponding concentration applied to their right ear. One hour after inflammation induction, the animals were sacrificed, and ear flaps were weighed to calculate the degree of swelling and inhibition rate. Results: The swelling level in the model control group was 18.7±2.3 mg; in the dexamethasone group it was 7.2±1.8 mg (inhibition rate 61.5%); and in the high, medium, and low dose groups of the composition of the present invention, the inhibition rates were 4.1±1.2 mg (inhibition rate 78.1%), 6.5±1.5 mg (inhibition rate 65.2%), and 9.8±1.9 mg (inhibition rate 47.6%), respectively. This indicates that the composition of the present invention has a significant anti-inflammatory effect, and the high-dose group is more effective than dexamethasone.

[0028] Experiment 2: Effect of carrageenan on paw edema in rats Fifty Wistar rats were randomly divided into groups as in Experiment 1. Each group of rats received a subcutaneous injection of 0.1 mL of 1% carrageenan into the right hind paw to induce inflammation. One hour before inflammation induction, the positive control group received indomethacin 5 mg / kg via gavage. For each dose group of the plant extract composition prepared in Example 1 of this invention (equivalent to final concentrations of 5%, 3%, and 1%, respectively), 0.2 mL of the corresponding concentration of the composition was applied to the right hind paw. Paw volume was measured at 1, 2, 3, 4, and 5 hours after inflammation induction, and the degree of swelling and inhibition rate were calculated.

[0029] Results: Four hours after inflammation induction, the swelling in the model control group was 0.82±0.12 mL; indomethacin group it was 0.31±0.08 mL (inhibition rate 62.2%); and the high, medium, and low dose groups of the composition of the present invention were 0.26±0.07 mL (inhibition rate 68.3%), 0.35±0.09 mL (inhibition rate 57.3%), and 0.48±0.10 mL (inhibition rate 41.5%), respectively. This indicates that the composition of the present invention has a good inhibitory effect on acute inflammation.

[0030] Experiment 3: Effects of cotton ball-induced granulomas in rats One hundred and twenty SD rats were sterilized and implanted subcutaneously in the axillae under aseptic conditions. They were randomly divided into four groups: a model control group, a positive control group (hydrocortisone), a group containing the plant extract composition prepared according to the present invention and comparative examples (5%), and a blank control group. The drugs were administered once daily for seven consecutive days post-surgery. On day 8, the granulation tissue from the cotton balls was removed, dried, and weighed. The weight of the granuloma was calculated, and the results are shown in Table 1.

[0031] Table 1. Granulation tissue weight and inhibition rate in mice The results in Table 1 show that the composition of the present invention also has a significant inhibitory effect on chronic inflammatory hyperplasia. The comparative example shows that the components of the present invention have a synergistic effect. The plant extract prepared by the method of the present invention has a high extraction rate of active ingredients, high purity, and more significant effect.

[0032] Experiment 4: Evaluation of Skin Soothing Effect Human trial: Ninety volunteers with sensitive skin (66 women and 24 men, aged 20-45 years) were recruited. They used a soothing serum containing 5% of the compositions prepared in the examples and comparative examples, twice daily for 28 consecutive days. The effects were evaluated through instrumental measurements and questionnaires; the results are shown in Table 2. Table 2. Transdermal water loss values ​​and skin erythema index As shown in Table 2, the subjects in the Example group showed significant improvement in skin sensitivity symptoms and no adverse reactions. However, the comparative group lacked a single component or ingredient of the composition and lacked the extraction and preparation process of the present invention, resulting in a reduced soothing effect.

Claims

1. A plant extract composition having anti-inflammatory and soothing efficacy, characterized in that, The composition comprises the following components by weight percentage: 1-5% of white radix bupleuri extract, 1-3% of jasminum lanceolatum flower extract, 1-5% of kochia scoparia flower extract, 0.5-1% of andrographis paniculata extract, 1-3% of citrus peel extract, 0.5-1% of yucca extract, and the rest is solvent. The solvent is one or more of water, glycerol, propylene glycol and butylene glycol.

2. The plant extract composition having anti-inflammatory and soothing efficacy according to claim 1, characterized in that, The white radix bupleuri extract is prepared as follows: the dried white radix bupleuri is crushed into coarse powder, 0.05-0.10 mol / L citric acid-sodium citrate buffer solution is added at a solid-liquid ratio of 1:8-12 g / mL, 0.05-0.1% (based on the weight of the white radix bupleuri powder) of enzyme complex is added for enzymolysis for 80-100 min, the extracted liquid is combined after reflux extraction, and the obtained extract is extracted with petroleum ether and ethyl acetate in sequence to obtain the white radix bupleuri extract.

3. The plant extract composition having anti-inflammatory and soothing efficacy according to claim 2, characterized in that, The enzyme complex is CaCl2, disodium ethylenediaminetetraacetate and complex enzyme at a mass ratio of 3:3:1-3; the complex enzyme is cellulase, hemicellulase and pectinase at a mass ratio of 2-4:2-4:

1.

4. The plant extract composition having anti-inflammatory and soothing efficacy according to claim 1, characterized in that, The jasminum lanceolatum flower extract is prepared as follows: the jasminum lanceolatum flower buds are dried to a water content of 6-8%, crushed to 60 mesh, and subjected to steam explosion at a pressure of 0.4-0.6 MPa for 40-50 s, then extracted with pure water at a pressure of 0.1-0.2 MPa for 20-30 min and at normal pressure for 40-50 min, the extracted liquid is combined, and the obtained product is subjected to molecular distillation after pressure reduction and compression.

5. The plant extract composition having anti-inflammatory and soothing efficacy as claimed in claim 1, wherein, The andrographis paniculata extract is prepared as follows: the dried andrographis paniculata whole plant powder is added to an extraction liquid at a solid-liquid ratio of 1:9-11 g / mL, heated and reflux extracted at a temperature of 60-70℃ for 40-50 min, extracted for 2-3 times, the extracted liquid is combined, and the obtained extract is dissolved and filtered, the filtrate is extracted with dichloromethane, and then purified by silica gel column chromatography.

6. The plant extract composition having anti-inflammatory and soothing efficacy as claimed in claim 5, wherein, The extraction liquid is citric acid-disodium hydrogen phosphate buffer solution containing 5-8% of mannitol and 2-3% of neutral aluminum oxide.

7. The plant extract composition having anti-inflammatory and soothing efficacy as claimed in claim 1, wherein, The citrus peel extract is prepared as follows: fresh citrus peel is freeze-dried at -20~-50℃, crushed to 40-100 mesh, added with a composite solvent at a solid-liquid ratio of 1:6-8, added with 0.05-0.1% of composite biological additives based on the weight of the peel, subjected to enzymolysis at a temperature of 40-45℃ for 80 min, then extracted at a power of 300 W and a temperature of 50℃ for 30 min, extracted for 2-3 times, the extracted liquid is combined, the solvent is recovered under reduced pressure, the pH is adjusted to 5.0-5.5, the crude extract is obtained by centrifugation, and then purified by a macroporous adsorption resin column, impurities are removed by elution with 20% ethanol, then eluted with 50% ethanol, the 50% ethanol elution part is collected, concentrated under reduced pressure, and spray dried to obtain the product.

8. The plant extract composition having anti-inflammatory and soothing efficacy as claimed in claim 7, wherein, The composite solvent is pure water containing 1-3:10-13 of citric acid-glucose by mass ratio; the composite biological additive is rhamnolipid and pectinase at a mass ratio of 4-7:

1.

9. The plant extract composition having anti-inflammatory and soothing efficacy as claimed in claim 1, wherein, Preparation of the extract of Paris polyphylla: take the dried rhizome of Paris polyphylla, crush it, and then add 4-6 times the amount of a eutectic solvent to reflux extract it 3 times, 2 hours each time, combine the extract, and reduce pressure to concentrate it; use a D101 macroporous resin column to elute the obtained extract with water, 30% ethanol, and 70% ethanol in sequence, and reduce pressure to dry the obtained eluate to obtain the extract.

10. The plant extract composition having anti-inflammatory and soothing efficacy as claimed in claim 1, wherein, Preparation of the extract of Hibiscus hamabo: take fresh Hibiscus hamabo flowers, add a mixed solvent of 20%-30% ethanol and 0.02%-0.04% acetic acid at a solid-liquid ratio of 1:5-8 g / mL, and then ultrasonically assist extraction under a nitrogen atmosphere at a power of 200-300 W and a temperature of 40-50°C for 20-30 min; after microfiltration through a 0.22 μm ceramic membrane, reduce pressure to concentrate the filtrate and then freeze-dry it to obtain the extract.

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