Composition with anti-wrinkle and firming effects and application

Abstract

The invention discloses a composition with anti-wrinkle and firming effects. The composition is prepared from the following components: a hemerocallis fulva flower extract, a lilium davidii linn / leaf extract, a fructus chebulae bark extract, a magnolia liliiflora bud extract, a phalaenopsis aphrodite extract, butanediol, hexanediol and water. The composition is safe, stable, efficient and mild, formed wrinkles and relaxation problems can be remarkably improved, new wrinkles can be prevented, through the synergistic effect of plant components, the irritation is greatly reduced while the effect is guaranteed, and sensitive skin can also safely use efficient anti-aging products.

Description

Technical Field

[0001] This invention discloses a composition with anti-wrinkle and firming effects, and also discloses the application of the composition with anti-wrinkle and firming effects, belonging to the field of daily chemical raw materials. Background Technology

[0002] In the cosmetics industry, anti-wrinkle and firming effects have always been a research focus, and compound formulas derived from natural plants are highly favored due to their gentle effects and synergistic effects on multiple targets.

[0003] In existing technologies, most anti-wrinkle and firming products rely on synthetic active ingredients (such as retinol and peptides). While these have some effect, long-term use can easily cause irritation and allergies, especially for sensitive skin. Some natural plant extracts (such as centella asiatica and aloe vera) have soothing and repairing effects, but their effectiveness in promoting collagen regeneration and inhibiting elastase and hyaluronidase, key anti-aging indicators, is limited.

[0004] Therefore, developing a highly effective and gentle composition with anti-wrinkle and firming effects to address the technical shortcomings of existing products has become an urgent problem for the industry. Summary of the Invention

[0005] To address the shortcomings of current methods, the first objective of this invention is to provide a composition with anti-wrinkle and firming effects. This composition is safe, stable, highly effective, and gentle, significantly improving existing wrinkles and sagging, and preventing the formation of new wrinkles. Through the synergistic effect of plant ingredients, it greatly reduces irritation while ensuring efficacy, making it a safe and effective anti-aging product for sensitive skin.

[0006] To achieve the above objectives, the specific technical solution is as follows: A composition with anti-wrinkle and firming effects, comprising daylily flower extract, lily of the valley orchid / leaf extract, phalaenopsis orchid extract, butylene glycol, hexanediol, and water.

[0007] Furthermore, its components also include Terminalia chebula bark extract.

[0008] Furthermore, its components also include extracts from magnolia flower buds.

[0009] Furthermore, it consists of the following components: daylily flower extract, *Liriodendron mucronatum* orchid / leaf extract, *Terminalia chebula* bark extract, *Magnolia purpurea* flower bud extract, *Phalaenopsis* orchid extract, butylene glycol, hexanediol, and water.

[0010] Further, it is composed of the following components in parts by weight: 1-3 parts daylily flower extract, 1-3 parts *Liriodendron mucronulatum* orchid / leaf extract, 3-5 parts *Terminalia chebula* bark extract, 10-20 parts *Magnolia denudata* flower bud extract, 3-10 parts *Phalaenopsis* orchid extract, 10-100 parts butylene glycol, 10-100 parts hexanediol, and 10-100 parts water.

[0011] Further, it is composed of the following components in parts by weight: 2 parts daylily flower extract, 2 parts *Liriope muscari* orchid / leaf extract, 4 parts *Terminalia chebula* bark extract, 15 parts *Magnolia purpurea* flower bud extract, 6 parts *Phalaenopsis* orchid extract, 55 parts butylene glycol, 55 parts hexanediol, and 55 parts water.

[0012] The second technical solution provided by the present invention is: the application of the above-mentioned composition with anti-wrinkle and firming effects in the preparation of cosmetics.

[0013] Furthermore, the cosmetic is a topical skin preparation made by using the aforementioned anti-wrinkle and firming composition as the active ingredient, plus conventional pharmaceutical or cosmetic excipients or auxiliary ingredients.

[0014] Furthermore, the cosmetics include one or more of the following: aqueous solutions, creams, cleansers, serums, masks, and mud masks.

[0015] Furthermore, the amount of the composition with anti-wrinkle and firming effects added to the cosmetic is 0.1%-10%.

[0016] Compared with the prior art, the present invention has the following beneficial effects:

[0017] 1. The plant-based ingredients are low in irritants, and the compound formula reduces the concentration of single ingredients, making it safer and suitable for long-term anti-aging use.

[0018] 2. Multi-dimensional and multi-target anti-aging, synergistically maintaining skin elasticity.

[0019] 3. When this composition is at a mass fraction of 2%, its addition to various functional dosage forms can significantly improve existing wrinkles and sagging, and prevent the formation of new wrinkles. Experimental data shows that the antioxidant efficacy of the four components combined is increased by approximately 50%. The collagen synthesis promotion effect is enhanced by 45%, and the transdermal absorption rate is increased by 30% compared to the single components. It is particularly worth mentioning that this combination, through the synergistic effect between plant ingredients, greatly reduces irritation while ensuring efficacy, allowing even sensitive skin to safely use highly effective anti-aging products. Detailed Implementation

[0020] The claims of the present invention will be further described in detail below with reference to specific embodiments, but this does not constitute any limitation on the present invention. Any limited modifications made by any person within the scope of protection of the claims of the present invention are still within the scope of protection of the claims of the present invention.

[0021] Example 1

[0022] A composition with anti-wrinkle and firming effects is prepared from the following raw materials: 0.1g of daylily flower extract, 0.1g of Lilium lancifolium / leaf extract, 0.3g of Terminalia chebula bark extract, 1g of Magnolia liliiflora bud extract, 0.3g of Phalaenopsis orchid extract, 1g of butylene glycol, 1g of hexanediol, and 1g of water.

[0023] Example 2

[0024] A composition with anti-wrinkle and firming effects is prepared from the following raw materials: 0.2g of daylily flower extract, 0.2g of Lilium lancifolium leaf extract, 0.4g of Terminalia chebula bark extract, 1.5g of Magnolia liliiflora bud extract, 0.6g of Phalaenopsis orchid extract, 5.5g of butylene glycol, 5.5g of hexanediol, and 5.5g of water.

[0025] Example 3

[0026] A composition with anti-wrinkle and firming effects is prepared from the following raw materials: 0.3g of daylily flower extract, 0.3g of Lilium lancifolium leaf extract, 0.5g of Terminalia chebula bark extract, 2g of Magnolia liliiflora bud extract, 1g of Phalaenopsis orchid extract, 10g of butylene glycol, 10g of hexanediol, and 10g of water.

[0027] Comparative Example 1

[0028] Compared with Example 1, the daylily flower extract was removed, but everything else remained unchanged.

[0029] Comparative Example 2

[0030] Compared with Example 1, the extract of Lilium lancifolium orchid / leaf was removed, while all other steps remained unchanged.

[0031] Comparative Example 3

[0032] Compared with Example 1, the Terminalia chebula bark extract was removed, while all other aspects remained unchanged.

[0033] Comparative Example 4

[0034] Compared with Example 1, the extract of purple magnolia flower buds was removed, while all other aspects remained unchanged.

[0035] Comparative Example 5

[0036] Compared with Example 1, the Phalaenopsis extract was removed, but everything else remained unchanged.

[0037] Table 1. Composition Table of Examples and Comparative Examples

[0038] Raw material / g Daylily extract Rain Lily Orchid / Leaf Extract Terminalia chebula bark extract Purple Magnolia Flower Bud Extract Phalaenopsis extract Butylene glycol Hexanediol water Example 1 0.1 0.1 0.3 1 0.3 1 1 1 Example 2 0.2 0.2 0.4 1.5 0.6 5.5 5.5 5.5 Example 3 0.3 0.3 0.5 2 1 10 10 10 Comparative Example 1 0 0.1 0.3 1 0.3 1 1 1 Comparative Example 2 0.1 0 0.3 1 0.3 1 1 1 Comparative Example 3 0.1 0.1 0 1 0.3 1 1 1 Comparative Example 4 0.1 0.1 0.3 0 0.3 1 1 1 Comparative Example 5 0.1 0.1 0.3 1 0 1 1 1

[0039] To better demonstrate the technical solution provided in this application, the following testing experiments are presented:

[0040] Experiment 1: Accelerated Stability Performance Test

[0041] This test is a one-month accelerated stability test of the compositions obtained in Examples 1-3 and Comparative Examples 1-3.

[0042] The composition prepared above was subjected to stability tests under the following conditions: room temperature, light exposure (natural light), low temperature (-15℃), high temperature (45℃), high and low temperature cycling (45℃ / -15℃ for 24 hours each), and refrigeration (5℃). The data are recorded in Table 2.

[0043] Table 2. Results of accelerated stability test of the composition over one month.

[0044] Group Example 1 Example 2 Example 3 Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 Comparative Example 5 Room temperature (away from light) √ √ √ √ √ √ √ √ Refrigerate (5℃) √ √ √ √ √ √ √ √ Cold-resistant (-15℃) √ √ √ √ √ √ √ √ Heat resistant (45℃) 30 days light yellow 30 days light yellow 30 days light yellow 30 days light yellow 30 days light yellow 30 days light yellow 30 days light yellow 30 days light yellow Lighting (North Light) Slight yellowing after 30 days Slight yellowing after 30 days Slight yellowing after 30 days Slight yellowing after 30 days Slight yellowing after 30 days Slight yellowing after 30 days Slight yellowing after 30 days Slight yellowing after 30 days High and low temperature cycling Slightly yellow in 30 days Slightly yellow in 30 days Slightly yellow in 30 days Slightly yellow in 30 days Slightly yellow in 30 days Slightly yellow in 30 days Slightly yellow in 30 days Slightly yellow in 30 days in conclusion Relatively stable Relatively stable Relatively stable Relatively stable Relatively stable Relatively stable Relatively stable Relatively stable

[0045] As can be seen from the experimental results in Table 3, there were no obvious abnormalities in the stability of Examples 1-3 and Comparative Examples 1-3, and the stability was passed.

[0046] Experimental Example 2: Human Skin Patch Test.

[0047] This test used the compositions obtained in Examples 1-3 and Comparative Examples 1-3, prepared as 10% aqueous solutions for testing. Following the human skin patch test in the "2022 Cosmetic Safety Technical Specifications," skin reactions were observed at 30 min (after the indentation disappeared), 24 h, and 48 h according to standards, and the results were recorded. The data are recorded in Table 3.

[0048] Table 3. Results of human skin patch test of the composition

[0049] serial number 30min 24h 48h Example 1 Level 0, 30 people Level 0, 30 people Level 0, 30 people Example 2 Level 0, 30 people Level 0, 30 people Level 0, 30 people Example 3 Level 0, 30 people Level 0, 30 people Level 0, 30 people Comparative Example 1 Level 0, 30 people Level 0, 30 people Level 0, 30 people Comparative Example 2 Level 0, 30 people Level 0, 30 people Level 0, 30 people Comparative Example 3 Level 0, 30 people Level 0, 30 people Level 0, 30 people Comparative Example 4 Level 0, 30 people Level 0, 30 people Level 0, 30 people Comparative Example 5 Level 0, 30 people Level 0, 30 people Level 0, 30 people

[0050] As shown in Table 3, the composition is non-irritating when added at a concentration of 10%, and can be used safely within this range.

[0051] Experimental Example 3: Collagenase Inhibition Test.

[0052] 3.1 Experimental Materials and Equipment

[0053] 3.1.1 Experimental reagents and materials: Collagenase inhibition kit

[0054] 3.1.2 Main instruments and equipment: shaking plate, fluorescence microplate reader.

[0055] 3.2 Experimental Methods

[0056] 3.2.1 Experimental Group Setup

[0057] BC – Solvent control group (solvent + substrate)

[0058] NC – Negative control group (enzyme + substrate)

[0059] PC – Positive control group (inhibitor + enzyme + substrate)

[0060] S—Test substance (test substance + enzyme + substrate)

[0061] 3.2.2 Experimental Procedure

[0062] Step 1: Preparation of test subjects;

[0063] Step 2: Add enzyme reagent and react (react at room temperature for 15 minutes);

[0064] Step 3: Add substrate and react in the dark (react at 37°C for 30-60 minutes);

[0065] Step 4: Measure fluorescence using a fluorescence microplate reader;

[0066] Step 5: Data processing.

[0067] 3.3 Result Calculation and Judgment

[0068] 3.3.1 Result Calculation

[0069] Formula for calculating collagenase inhibition rate:

[0070] Inhibition rate = [1 - (MFIS - MFIBC)) / (MFINC - MFIBC)] * 100%

[0071] in:

[0072] MFIBC represents the average fluorescence intensity value of the solvent control group;

[0073] MFINC represents the mean fluorescence intensity value of the negative control group;

[0074] MFIS represents the average fluorescence intensity value of the test group; statistical and significance analysis was performed on the data, and the P value was calculated. P < 0.05 indicates a significant difference, otherwise there is no statistical difference.

[0075] 3.3.2 Result Determination

[0076] The experiment is considered valid if the fluorescence intensity of the negative control group is significantly different from that of the solvent control group (P < 0.05) and the inhibition rate of the positive control is ≥ 80%.

[0077] When the collagenase inhibition rate of the sample group was significantly higher than that of the negative control group, and the difference was statistically significant (P < 0.05), it can be determined that the sample has the ability to inhibit collagenase activity.

[0078] 3.4 Experimental Results

[0079] The fluorescence intensity of the negative control group was significantly different from that of the solvent control group (P < 0.05), and the inhibition rate of the positive control was 99.2% ≥ 80%, indicating that the experiment was effective.

[0080] The sample at 100% concentration showed an inhibition rate of 13.46% against collagen, which was significantly different from the negative control (P < 0.05). The sample also demonstrated the ability to inhibit protease activity; details are shown in Table 4.

[0081] Table 4. Results of Collagenase Inhibition Test

[0082] Group fluorescence intensity Collagen inhibition rate (%) Inhibition rate standard deviation p-value Significant difference Solvent control group 2230.50 100.00 0.622 0.0001 **** negative control group 31889.00 0.00 0.920 / / Positive control group 2467.50 99.20 1.132 0.0001 **** Example 1 27897.00 13.46 0.448 0.0029 ** Example 2 26153.80 18.00 0.396 0.0015 ** Example 3 24982.50 21.65 0.512 0.0008 *** Comparative Example 1 30125.70 5.53 0.684 0.0312 * Comparative Example 2 29876.30 6.31 0.598 0.0287 * Comparative Example 3 28954.20 9.20 0.483 0.0145 * Comparative Example 4 29532.60 7.39 0.615 0.0243 * Comparative Example 5 29218.40 8.38 0.537 0.0189 *

[0083] Note: If P > 0.05, it indicates no significant difference, denoted as "ns"; if P < 0.05, it indicates a significant difference, and 0.01 < P ≤ 0.05 is denoted as "*"; 0.01 < P ≤ 0.01 is denoted as "**"; 0.0001 < P ≤ 0.001 is denoted as "***"; P ≤ 0.0001 is denoted as "****".

[0084] 3.5 Experimental Conclusions

[0085] Results Analysis

[0086] 1. The inhibition rates of all examples and comparative examples were significantly higher than those of the negative control group (P < 0.05), and the inhibition rate of the positive control group was ≥ 80%, indicating that the experiment was effective;

[0087] 2. The inhibition rate of the example group was significantly higher than that of the control group, with Example 3 (high ratio group) showing the best inhibition effect (21.65%), demonstrating the synergistic effect of the combination of five plant extracts;

[0088] 3. The control groups, lacking a single plant extract, all had inhibition rates below 10%, indicating that each plant extract plays a crucial role in the anti-wrinkle and firming effects, and none can be omitted.

[0089] The above embodiments are preferred embodiments of the present invention, but the embodiments of the present invention are not limited to the above embodiments. Any changes, modifications, substitutions, combinations, or simplifications made without departing from the spirit and principle of the present invention should be considered equivalent substitutions and are included within the protection scope of the present invention.

Claims

1. A composition having anti-wrinkle and firming effects, characterized in that, Its components include: daylily flower extract, lily of the valley orchid / leaf extract, phalaenopsis orchid extract, butylene glycol, hexanediol, and water.

2. The composition with anti-wrinkle and firming effects according to claim 1, characterized in that, Its components also include Terminalia chebula bark extract.

3. The composition with anti-wrinkle and firming effects according to claim 1, characterized in that, Its components also include extracts from magnolia flower buds.

4. The composition with anti-wrinkle and firming effects according to claim 1, characterized in that, It consists of the following components: daylily flower extract, lily of the valley orchid / leaf extract, Terminalia chebula bark extract, magnolia flower bud extract, phalaenopsis orchid extract, butylene glycol, hexanediol, and water.

5. The composition with anti-wrinkle and firming effects according to claim 1, characterized in that, It consists of the following components in parts by weight: 1-3 parts daylily flower extract, 1-3 parts *Liriodendron mucronulatum* orchid / leaf extract, 3-5 parts *Terminalia chebula* bark extract, 10-20 parts *Magnolia lily* flower bud extract, 3-10 parts *Phalaenopsis* orchid extract, 10-100 parts butylene glycol, 10-100 parts hexanediol, and 10-100 parts water.

6. The composition with anti-wrinkle and firming effects according to claim 1, characterized in that, It consists of the following components by weight: 2 parts daylily flower extract, 2 parts *Liriope muscari* orchid / leaf extract, 4 parts *Terminalia chebula* bark extract, 15 parts *Magnolia purpurea* flower bud extract, 6 parts *Phalaenopsis* orchid extract, 55 parts butylene glycol, 55 parts hexanediol, and 55 parts water.

7. The application of a composition having anti-wrinkle and firming effects according to any one of claims 1-6, characterized in that, The composition with anti-wrinkle and firming effects is used in the preparation of cosmetics.

8. The application according to claim 7, characterized in that: The cosmetic is a topical skin preparation made by using the aforementioned anti-wrinkle and firming composition as the active ingredient, plus conventional pharmaceutical or cosmetic excipients or auxiliary ingredients.

9. The application according to claim 7, characterized in that, The cosmetics include one or more of the following: liquids, creams, cleansers, serums, masks, and mud masks.

10. The application according to claim 7, characterized in that, The amount of the anti-wrinkle and firming composition added to the cosmetic product is 0.1%-10%.