Beta-glucan composition for regulating micro-ecology of skin as well as preparation method and application of beta-glucan composition

Abstract

The invention discloses a beta-glucan composition for regulating skin micro-ecology as well as a preparation method and application thereof, and belongs to the technical field of cosmetics. The beta-glucan composition disclosed by the invention is prepared from the following components in percentage by mass: 0.2 to 0.6 percent of beta-glucan, 1 to 3 percent of a streptococcus thermophilus fermentation product and 0.05 to 0.15 percent of capryloyl hydroximic acid. The beta-glucan composition prepared by the invention is mild in formula and can achieve a rapid anti-inflammatory effect. By adopting the synergistic effect of the beta-glucan, the streptococcus thermophilus fermentation product and the capryloyl hydroximic acid, common adverse flora on the skin surface is inhibited, inflammatory factors are reduced, and the micro-ecological balance of the skin is rebuilt.

Description

Technical Field

[0001] This invention belongs to the field of cosmetic technology, specifically relating to a β-glucan composition for regulating skin microecology, its preparation method, and its application. Background Technology

[0002] The skin's microecological environment is composed of various microorganisms, exfoliated cells from the stratum corneum of the skin surface, and secretions from various sweat glands and sebaceous glands. It maintains the ecological balance between the human microbiome and various skin cells and tissues, playing a vital role in the skin's immune and regulatory systems. When significant changes occur in the internal and external environment, this ecological balance is disrupted, leading to microecological dysbiosis and triggering skin diseases such as atopic dermatitis, acne, psoriasis, or other infectious conditions. By regulating and guiding the skin's microecological environment daily, it is possible to alleviate mild symptoms and restore healthy skin. Therefore, this invention provides a composition that can be applied to various skincare products, integrating the regulation of the skin's microecological environment into daily life. Summary of the Invention

[0003] The purpose of this section is to outline some aspects of embodiments of the present invention and to briefly describe some preferred embodiments. Simplifications or omissions may be made in this section, as well as in the abstract and title of this application, to avoid obscuring the purpose of these documents; however, such simplifications or omissions should not be construed as limiting the scope of the invention.

[0004] In view of the problems existing in the above and / or prior art, the present invention is proposed.

[0005] Therefore, the purpose of this invention is to overcome the shortcomings of existing skin care products in regulating the skin microecology, which are slow to take effect and have poor results, and to provide a β-glucan composition for regulating the skin microecology.

[0006] To solve the above-mentioned technical problems, the present invention provides the following technical solution: a β-glucan composition for regulating skin microecology, comprising, by mass fraction, 0.2-0.6% β-glucan, 1-3% Streptococcus thermophilus fermentation product, and 0.05-0.15% capryloyl hydroxamic acid.

[0007] As a preferred embodiment of the β-glucan composition for regulating skin microecology according to the present invention, the composition comprises, by mass fraction, 0.5% β-glucan, 2% Streptococcus thermophilus fermentation product, and 0.1% capryloyl hydroxamic acid.

[0008] As a preferred embodiment of the β-glucan composition for regulating skin microecology according to the present invention, wherein the molecular weight of the β-glucan is 2000~3000 kDa.

[0009] As a preferred embodiment of the β-glucan composition for regulating skin microecology according to the present invention, the composition further includes the balance being a solvent.

[0010] As a preferred embodiment of the β-glucan composition for regulating skin microecology according to the present invention, the solvent includes one or more of water, ethanol, 1,2-propanediol, 1,3-propanediol, 1,2-butanediol, 1,3-butanediol, 1,2-pentanediol, 1,5-pentanediol, isopentanediol, 1,2-hexanediol, isohexanediol, 1,2-hexanol, dipropylene glycol, glycerol, and methylpropanediol.

[0011] Another objective of this invention is to overcome the shortcomings of existing skincare products in regulating skin microecology, which are characterized by slow onset and poor efficacy, and to provide a method for preparing a β-glucan composition for regulating skin microecology. This preparation method includes: β-glucan and octanoyl hydroxamic acid were pre-dissolved in solvents and then mixed evenly. After the system was cooled to below 28°C, it was mixed evenly with the fermentation product of Streptococcus thermophilus and water was added to obtain the β-glucan composition.

[0012] As a preferred embodiment of the preparation method of the β-glucan composition for regulating skin microecology according to the present invention, the preparation method of the Streptococcus thermophilus fermentation product includes: activating the Streptococcus thermophilus strain, inoculating it into a lactic acid bacteria culture medium, culturing it at 42±1℃ for 48~72h, and sterilizing it at 115~120℃ for 15~20min to obtain the Streptococcus thermophilus fermentation product.

[0013] Another objective of this invention is to overcome the shortcomings of the prior art and provide a skin care product containing the aforementioned composition.

[0014] As a preferred embodiment of the skin care product of the present invention, the composition accounts for 1 to 10% of the skin care product by mass fraction.

[0015] As a preferred embodiment of the skincare product described in this invention, the skincare product includes one of the following: facial mask liquid, lotion, cream, toner, serum, makeup remover, essence, facial cleanser, lotion, foundation, foundation cream, and concealer.

[0016] Beneficial effects of this invention: The β-glucan composition prepared by this invention has a mild formulation that can achieve a rapid anti-inflammatory effect, with IL-1 levels decreasing by ≥20% after 14 days of use. This invention utilizes the synergistic effect of β-glucan, Streptococcus thermophilus fermentation products, and capryloyl hydroxamic acid to inhibit common harmful bacteria on the skin surface, reduce inflammatory factors, and restore the skin's microecological balance. Detailed Implementation

[0017] To make the above-mentioned objects, features and advantages of the present invention more apparent and understandable, the specific embodiments of the present invention will be described in detail below with reference to the examples in the specification.

[0018] Many specific details are set forth in the following description in order to provide a full understanding of the invention. However, the invention may also be practiced in other ways different from those described herein, and those skilled in the art can make similar extensions without departing from the spirit of the invention. Therefore, the invention is not limited to the specific embodiments disclosed below.

[0019] Secondly, the term "an embodiment" or "embodiment" as used herein refers to a specific feature, structure, or characteristic that may be included in at least one implementation of the present invention. The phrase "in one embodiment" appearing in different places throughout this specification does not necessarily refer to the same embodiment, nor is it a single embodiment or an embodiment selectively excluded from other embodiments.

[0020] Unless otherwise specified, all raw materials used in this invention are commercially available.

[0021] β-glucan is celecoxib glucan produced by Sichuan Hetai Xinguang Biotechnology Co., Ltd.

[0022] Streptococcus thermophilus was purchased from the China Industrial Microbial Culture Collection Center, strain number CICC 20366.

[0023] Example 1

[0024] This embodiment provides a β-glucan composition for regulating skin microecology, specifically: The formulation of the β-glucan composition by mass fraction is as follows: β-glucan 0.5%, Streptococcus thermophilus fermentation product 2%, capryloyl hydroxamic acid 0.1%, water, 1,2-propanediol.

[0025] A method for preparing a β-glucan composition includes the following steps: Prepare the lactic acid bacteria culture medium according to the following formula: yeast extract 7.5 g / L, glucose 10 g / L, tomato juice 100 mL / L, peptone 7.5 g / L, KH2PO4 2 g / L, Tween 80 0.5 mL / L, and distilled water to make up the volume. Sterilize at 121℃ for 15 min before use.

[0026] After activating and transferring the Streptococcus thermophilus strain, it was inoculated into the above-mentioned culture medium and cultured at 42°C for 48 hours. After the culture was completed, it was sterilized at 115°C for 15 minutes to obtain the Streptococcus thermophilus fermentation product.

[0027] β-glucan and octanoyl hydroxamic acid were pre-dissolved in water and 1,2-hexanediol, respectively, and then mixed evenly. After the system was cooled to below 28°C, it was mixed with the fermentation product of Streptococcus thermophilus and water was added to obtain the β-glucan composition.

[0028] Example 2

[0029] The difference between this embodiment and Example 1 is that the content of β-glucan in the raw materials is adjusted to 0.2%, while the rest of the preparation process is the same as in Example 1, to obtain a β-glucan composition.

[0030] Example 3

[0031] The difference between this embodiment and Example 1 is that the content of β-glucan in the raw materials is adjusted to 0.6%, while the rest of the preparation process is the same as in Example 1, to obtain a β-glucan composition.

[0032] Comparative Example 1

[0033] The difference between this comparative example and Example 1 is that the content of β-glucan in the raw materials was adjusted to 0%, while the rest of the preparation process was the same as in Example 1, and a β-glucan composition was obtained.

[0034] Comparative Example 2

[0035] The difference between this comparative example and Example 1 is that the content of β-glucan in the raw materials was adjusted to 1%, while the rest of the preparation process was the same as in Example 1, and a β-glucan composition was obtained.

[0036] Example 4

[0037] The difference between this embodiment and Example 1 is that the content of thermophilic streptococcal fermentation product in the raw materials is adjusted to 1%, while the rest of the preparation process is the same as in Example 1, to obtain the β-glucan composition.

[0038] Example 5

[0039] The difference between this embodiment and Example 1 is that the content of thermophilic streptococcal fermentation product in the raw materials is adjusted to 3%, while the rest of the preparation process is the same as in Example 1, to obtain the β-glucan composition.

[0040] Comparative Example 3

[0041] The difference between this comparative example and Example 1 is that the content of thermophilic streptococcal fermentation product in the raw materials was adjusted to 0%, while the rest of the preparation process was the same as in Example 1, to obtain the β-glucan composition.

[0042] Comparative Example 4

[0043] The difference between this comparative example and Example 1 is that the content of thermophilic streptococcal fermentation product in the raw materials was adjusted to 0.5%, while the rest of the preparation process was the same as in Example 1, to obtain the β-glucan composition.

[0044] Comparative Example 5

[0045] The difference between this comparative example and Example 1 is that the content of thermophilic streptococcal fermentation product in the raw materials was adjusted to 4%, while the rest of the preparation process was the same as in Example 1, to obtain the β-glucan composition.

[0046] Example 6

[0047] The difference between this embodiment and Example 1 is that the content of octanoyl hydroxamic acid in the raw materials is adjusted to 0.05%, while the rest of the preparation process is the same as in Example 1, to obtain the β-glucan composition.

[0048] Example 7

[0049] The difference between this embodiment and Example 1 is that the content of octanoyl hydroxamic acid in the raw materials is adjusted to 0.15%, while the rest of the preparation process is the same as in Example 1, to obtain the β-glucan composition.

[0050] Comparative Example 6

[0051] The difference between this comparative example and Example 1 is that the content of octanoyl hydroxamic acid in the raw materials was adjusted to 0, while the rest of the preparation process was the same as in Example 1, to obtain the β-glucan composition.

[0052] The performance of the β-glucan compositions prepared in the above examples and comparative examples was tested.

[0053] 1) The Oxford cup method was used to test the inhibition zone: Commonly prevalent harmful bacteria on the skin surface, such as Pseudomonas aeruginosa, Staphylococcus aureus, and Escherichia coli, were selected to test the inhibition zone. An inhibition zone diameter > 20 mm was recorded as ++++; a diameter of 15-20 mm as +++; a diameter of 10-15 mm as ++; a diameter < 10 mm as +; and a diameter of 0 mm as -. The results are shown in the table below:

[0054] Table 1. Inhibition zone test results for different groups against Pseudomonas aeruginosa, Staphylococcus aureus, and Escherichia coli. Pseudomonas aeruginosa Staphylococcus aureus E. coli Example 1 ++ ++ ++ Example 2 ++ + ++ Example 3 ++ ++ + Example 4 + ++ ++ Example 5 + ++ ++ Example 6 ++ + ++ Example 7 ++ ++ +

[0055] As shown in the table above, the β-glucan composition prepared in this invention has a good control effect on common unfavorable bacteria on the skin surface, and can significantly inhibit Gram-positive and Gram-negative bacteria, such as Pseudomonas aeruginosa, Staphylococcus aureus, and Escherichia coli; thus achieving regulation and control of various unfavorable bacteria on the skin surface and regulating the skin microecology. Among them, the composition prepared in Example 1 has the best effect, in which the mass ratio of β-glucan, Streptococcus thermophilus fermentation product, and capryloyl hydroxamic acid is 0.5∶2∶0.1.

[0056] Volunteers with mild facial acne who have not recently used any medication were recruited and screened. Symptoms included papules, comedones, scars, or nodules, large pores, excessive sebum production, and localized tenderness. After signing an informed consent form, volunteers received and used the sample at home continuously as instructed, with testing on days 0, 14, and 28.

[0057] 2) Facial sebum secretion rate (SER) was measured using a direct sebum weight measurement method. After removing sebum from a 1.0 cm × 1.0 cm area in the center of the cheek with 75% alcohol, the subject sat still for 3 hours. The skin in this area was repeatedly wiped with disposable cotton swabs, and the mass of the swabs before and after liposuction was recorded. SER [ug / (cm)] 2 ·min)] = (mass of liposuction swabs after liposuction - mass of liposuction swabs before liposuction) / (area × time).

[0058] Table 2 Facial sebum secretion rate of different groups [ug / (cm)] 2 ·min)] D0 D14 D28 Example 1 2.68 1.79 1.68 Example 2 2.55 1.95 1.83 Example 3 2.81 2.03 1.96 Example 4 2.61 2.08 1.89 Example 5 2.76 2.01 1.89 Example 6 2.75 2.14 1.93 Example 7 2.63 1.81 1.69 Comparative Example 1 2.73 2.58 2.49 Comparative Example 2 2.64 2.37 2.25 Comparative Example 3 2.79 2.68 2.62 Comparative Example 4 2.52 2.41 2.38 Comparative Example 5 2.58 2.45 2.38 Comparative Example 6 2.84 2.67 2.54

[0059] As shown in the table above, applying the β-glucan composition prepared in the embodiments of the present invention significantly reduced facial sebum secretion rate. After 14 days of use, the sebum secretion rate (SER) decreased by 20.3%–33.2%, and continued to improve after 28 days, with a further reduction in SER, reaching a maximum reduction of 37.3%. This indicates that the β-glucan composition prepared in this invention has a positive effect on improving acne.

[0060] 3) Test the local IL-1 level of the skin lesion: After disinfection with 75% alcohol, use a sterile comedone extractor to squeeze out the contents of the skin lesion, such as papules or cysts, and perform the test according to the operating procedure of the ELISA kit.

[0061] Table 3. Local IL-1 levels (pg / mL) in different groups of skin lesions D0 D14 D28 Example 1 210.55 130.48 120.58 Example 2 214.68 155.12 146.12 Example 3 213.81 141.83 130.91 Example 4 214.87 170.21 168.66 Example 5 213.76 168.95 161.38 Example 6 211.69 169.12 167.29 Example 7 211.63 166.18 162.61 Comparative Example 1 212.73 196.37 188.73 Comparative Example 2 211.64 182.09 171.27 Comparative Example 3 214.79 198.67 187.84 Comparative Example 4 210.52 179.54 172.45 Comparative Example 5 210.58 186.89 182.77 Comparative Example 6 214.84 206.45 200.54

[0062] As shown in the table above, the IL-1 level, an inflammatory marker at the site of skin lesions, was significantly reduced after using the β-glucan composition prepared according to the present invention. After 28 days of use, Example 1 reduced IL-1 from 210.55 pg / mL to 120.58 pg / mL, a relative reduction of 42.7% (p < 0.01). The composition prepared in Example 1 of the present invention reduced IL-1 levels to 130.5 pg / mL on day 14 and further to 120.6 pg / mL on day 28, indicating that the anti-inflammatory effect appeared and continued to strengthen within 2 weeks. The IL-1 levels in Comparative Example 1 (lacking β-glucan), Comparative Example 3 (lacking fermentation product), and Comparative Example 6 (lacking capryloyl hydroxamic acid) at 28 days were 188.7, 187.8, and 200.5 pg / mL, respectively, with no significant difference from the blank, proving that the three components work synergistically and are indispensable.

[0063] It should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit it. Although the present invention has been described in detail with reference to preferred embodiments, those skilled in the art should understand that modifications or equivalent substitutions can be made to the technical solutions of the present invention without departing from the spirit and scope of the technical solutions of the present invention, and all such modifications or substitutions should be covered within the scope of the claims of the present invention.

Claims

1. A β-glucan composition for regulating skin microecology, characterized in that: By mass fraction, it includes 0.2-0.6% β-glucan, 1-3% Streptococcus thermophilus fermentation product, and 0.05-0.15% octanoyl hydroxamic acid.

2. The β-glucan composition for regulating skin microecology as described in claim 1, characterized in that: By mass fraction, it includes 0.5% β-glucan, 2% Streptococcus thermophilus fermentation product, and 0.1% octanoyl hydroxamic acid.

3. The β-glucan composition for regulating skin microecology as described in claim 1, characterized in that: The molecular weight of the β-glucan is 2000~3000 kDa.

4. The β-glucan composition for regulating skin microecology as described in claim 1, characterized in that: The composition also includes the balance of solvent.

5. The β-glucan composition for regulating skin microecology as described in claim 4, characterized in that: The solvent includes one or more of the following: water, ethanol, 1,2-propanediol, 1,3-propanediol, 1,2-butanediol, 1,3-butanediol, 1,2-pentanediol, 1,5-pentanediol, isopentanediol, 1,2-hexanediol, isohexanediol, 1,2-hexanol, dipropylene glycol, glycerol, and methylpropanediol.

6. A method for preparing a β-glucan composition for regulating skin microecology as described in any one of claims 1 to 5, characterized in that: include, β-glucan and octanoyl hydroxamic acid were pre-dissolved in solvents and then mixed evenly. After the system was cooled to below 28°C, it was mixed evenly with the fermentation product of Streptococcus thermophilus and water was added to obtain the β-glucan composition.

7. The method for preparing the β-glucan composition for regulating skin microecology as described in claim 6, characterized in that: The method for preparing the thermophilic streptococcal fermentation product includes, After activating the Streptococcus thermophilus strain, it was inoculated into a lactic acid bacteria culture medium, cultured at 42±1℃ for 48~72h, and then sterilized at 115~120℃ for 15~20min to obtain the Streptococcus thermophilus fermentation product.

8. The use of a β-glucan composition as described in any one of claims 1 to 5 in a skin care product.

9. The application of the β-glucan composition as described in claim 8 in skincare products, characterized in that: The composition in the skin care product accounts for 1 to 10% by mass fraction.

10. The application of the β-glucan composition as described in claim 9 in skincare products, characterized in that: The skincare products include one of the following: facial mask liquid, lotion, cream, toner, serum, makeup remover, essence, facial cleanser, lotion, foundation, foundation cream, and concealer.