Vitamin K2 extraction device and method

Abstract

The invention relates to the technical field of vitamin preparation, and provides a vitamin K2 extraction device and method.The vitamin K2 extraction device comprises a fermentation tank, the fermentation tank is fixedly installed on a fixed rack, a feeding port is formed in the top of the fermentation tank, the output end of the fermentation tank is communicated with a discharging pipe, a control valve is fixedly installed on the discharging pipe, and the control valve is connected with the feeding port. The stirring shaft is rotatably mounted in the fermentation tank, the sampling barrel is fixedly mounted on the side part of the fermentation tank, the sampling assembly is mounted on the sampling barrel, the driving assembly is mounted at the top of the fermentation tank, and the ventilation assembly is mounted at the top of the fermentation tank; the technical problem that in the prior art, when fermentation liquor is sampled, due to the fact that the fermentation liquor retained in a sampling pipe is difficult to reflect the real change condition in a fermentation tank, the authenticity of the taken-out sample is affected, and the judgment on the fermentation condition is affected is solved.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of vitamin preparation, in particular, to a vitamin K2 extraction device and method. BACKGROUND

[0002] Vitamin K2, also known as menaquinone, is an endogenous fat-soluble vitamin, which is the only active form of vitamin K (K1, K2, K3, K4). Its main function is to maintain normal blood clotting. Vitamin K is a commonly used hemostatic drug in clinical practice. It can treat bleeding caused by vitamin K deficiency, promote the formation of prothrombin, and accelerate blood clotting. Vitamin K2 can also help convert blood calcium into bone calcium, so it also has a certain effect on treating and preventing osteoporosis, can increase bone density, prevent fractures, and can be used in combination with calcium tablets. Vitamin K2 can activate matrix glutamate protein and be used in combination with statin tablets to prevent atherosclerotic plaques, which is beneficial to cardiovascular protection.

[0003] In the preparation of vitamin K2, in the prior art, first, the culture medium is put into the fermentation tank and high-temperature sterilization is carried out, and the inoculation of the strain is carried out under sterile conditions. After inoculation is completed, a certain amount of air is introduced, and the pressure and temperature of the fermentation tank are stabilized for cultivation (during which the temperature and dissolved oxygen content are automatically controlled by the automatic control system). After more than 100 hours of cultivation (during which laboratory personnel take samples for testing multiple times), the cultivation is completed, and then the fermentation broth is directly sent to the concentrator for concentration without storage, so that the volume of the fermentation broth reaches the predetermined concentration. Then the concentrated liquid is sent to the spray drying tower to convert it into a powdery product. The bottom of the drying tower is the discharge port, and the discharge port is followed by a cyclone separator to collect the finished powder. The tail gas after separation is treated and discharged into the atmosphere.

[0004] However, in the preparation process, since the fermentation of the strain requires multiple sampling and testing of the fermentation broth, the sampling valve needs to be opened multiple times, and the sampling bottle needs to be connected and detached. If the sampling tube is long, the fermentation broth retained in the sampling tube will not accurately reflect the actual changes in the fermentation tank, which will affect the authenticity of the sample and thus affect the judgment of the fermentation condition. SUMMARY

[0005] To overcome the above-mentioned defects, the embodiments of the present application provide a vitamin K2 extraction device and method, which solve the technical problem that in the prior art, when the fermentation broth is sampled, the fermentation broth retained in the sampling tube cannot accurately reflect the actual changes in the fermentation tank, which affects the authenticity of the sample and thus affects the judgment of the fermentation condition.

[0006] The technical solution of the present application is as follows: According to one aspect, at least one embodiment of the present application provides a vitamin K2 extraction device, comprising a fermentation tank fixedly installed on a fixed rack, a feed inlet installed on the top of the fermentation tank, and a discharge pipe communicated with the output end of the fermentation tank, wherein a control valve is fixedly installed on the discharge pipe, and the device further comprises: a stirring shaft rotatably installed inside the fermentation tank; a sampling cylinder fixedly installed on the side of the fermentation tank; a sampling assembly installed on the sampling cylinder for sampling the fermentation liquid during fermentation; a driving assembly installed on the top of the fermentation tank for driving the stirring shaft and the sampling assembly; a ventilation assembly installed on the top of the fermentation tank for maintaining the air inlet during fermentation; a stirring assembly installed on the stirring shaft for stirring the fermentation liquid.

[0007] On the basis of the foregoing scheme, the sampling assembly comprises: an impeller coaxially rotatably installed inside the sampling cylinder; a liquid inlet pipe, one end of which is communicated with the upper part of the sampling cylinder, and the other end of which is communicated with the side wall of the fermentation tank; a sampling valve provided at the bottom of the sampling cylinder, one end of which is communicated with the lower part of the sampling cylinder through a first connecting pipe, and the other end of which is communicated with the side wall of the fermentation tank through a second connecting pipe; a sampling part installed at the bottom of the sampling valve for collecting samples.

[0008] On the basis of the foregoing scheme, the sampling part comprises: a sampling bottle provided at the output end of the sampling valve; a bottle cap installed at the bottle mouth of the sampling bottle, which is communicated with the output end of the sampling valve.

[0009] On the basis of the foregoing scheme, the driving assembly comprises: a mounting rack fixedly installed on the top of the fermentation tank; a driving pulley and a driven pulley rotatably installed inside the mounting rack, wherein the driven pulley is coaxially fixedly connected with the top of the stirring shaft; a transmission belt tightly sleeved between the driving pulley and the driven pulley; A motor is fixedly installed on the top of the installation rack, and an output end of the motor is coaxially and fixedly connected with the driving pulley. A transmission part is installed on the bottom of the installation rack, and is used to drive the impeller to rotate.

[0010] Based on the foregoing scheme, the transmission part comprises: A transmission shaft is coaxially and fixedly installed on the bottom of the driving pulley, and a bottom end of the transmission shaft is coaxially and fixedly connected with the impeller. An installation cylinder is rotatably installed on the outside of the transmission shaft, and the installation cylinder is fixedly installed between the installation rack and the sampling cylinder.

[0011] Based on the foregoing scheme, the ventilation assembly comprises: A gas pump is communicated with the top of the fermentation tank through a gas pipe. An exhaust valve is communicated with the top of the fermentation tank. An air guide part is arranged at the output end of the gas pipe, and the air guide part is installed on the inner top wall of the fermentation tank, and is used to guide the air entering the fermentation tank.

[0012] Based on the foregoing scheme, the air guide part comprises: A hemispherical shell is arranged at the output end of the gas pipe, and the hemispherical shell is fixedly installed on the inner top wall of the fermentation tank. A plurality of guide grooves are circumferentially and equally arranged on the hemispherical shell.

[0013] Based on the foregoing scheme, the stirring assembly comprises: Two stirring frames are symmetrically and fixedly installed on the stirring shaft, and gaps exist between the side portions of the two stirring frames and the inner side wall of the fermentation tank. A plurality of stirring plates are fixedly installed in the two stirring frames at equal intervals, and each stirring plate is arranged obliquely.

[0014] Based on the foregoing scheme, the side of the stirring frame close to the inner side wall of the fermentation tank is arranged as an inclined surface.

[0015] A vitamin K2 extraction method using the vitamin K2 extraction device comprises the following steps: Firstly, inoculation is performed, the inside of the fermentation tank is sterilized at high temperature, and then the strain is fed into the fermentation tank through the feeding port for inoculation. Secondly, air is supplied, the gas pump is started, and the gas pump sends air into the gas pipe, and the air is continuously sent into the inside of the fermentation tank through the gas pipe. Third step, air pressure regulation, adjust the opening pressure of the exhaust valve, when the fermentation tank reaches a certain pressure, the exhaust valve can be opened to discharge excess air; Fourth step, stirring, start the motor, the motor drives the driving pulley to rotate, the driving pulley drives the driven pulley to rotate through the transmission belt, thereby driving the stirring shaft to rotate through the driven pulley, and with the rotation of the stirring shaft, the stirring frame and the stirring plate are driven to rotate, and the fermentation liquid is stirred. Fifth step, push, with the rotation of the driving pulley, the driving pulley drives the impeller to rotate through the transmission shaft, providing a downward thrust for the fermentation liquid in the sampling cylinder. Sixth step, circulation, the fermentation liquid in the fermentation tank enters the inside of the sampling cylinder through the liquid inlet pipe, then passes through the first connecting pipe, the sampling valve and the second connecting pipe in turn, and finally returns to the inside of the fermentation tank, forming a circulation. Seventh step, sampling, install the sampling bottle at the bottom of the bottle cap, then open the sampling valve, and the fermentation liquid can be sampled, after a certain amount of fermentation liquid is taken out, the sampling valve is closed, the sampling bottle is removed from the bottom of the sampling valve, and the fermentation liquid is sent to the inspection link for testing until the culture is completed. Eighth step, concentration, the fermentation liquid after completing the culture is directly concentrated. Ninth step, powder making, when the fermentation liquid is concentrated to a certain concentration, it is dried and powdered through the spray drying tower, the bottom of the drying tower is a discharge port, and the discharge port is followed by a cyclone separator to collect finished powder, and the separated tail gas is discharged into the atmosphere after treatment.

[0016] The beneficial effects of the present application are: 1、In the present application, when the fermentation liquid is stirred with the rotation of the stirring shaft, the stirring shaft drives the stirring frame and the stirring plate to rotate, and since the side of the stirring frame close to the inner wall of the fermentation tank is provided with an inclined surface, the fermentation liquid will be affected by the inclined surface to generate vortex when passing through the side of the stirring frame, thereby achieving more efficient and more uniform mixing of the fermentation liquid and improving the uniformity of fermentation.

[0017] 2、In the present application, the impeller provides a downward thrust for the fermentation liquid in the sampling cylinder, at this time, the liquid in the sampling cylinder, the liquid inlet pipe, the sampling valve, the first connecting pipe and the second connecting pipe flows, the flowing direction is that the fermentation liquid in the fermentation tank enters the inside of the sampling cylinder through the liquid inlet pipe, then passes through the first connecting pipe, the sampling valve and the second connecting pipe in turn, and finally returns to the inside of the fermentation tank, forming a circulation, thereby ensuring that the sample taken during sampling can better represent the fermentation condition of the whole fermentation liquid in the fermentation tank, so that the test result is closer to the true situation of fermentation, and the degree of fermentation is easy to judge.

[0018] 3. In this invention, by setting up the driving component, the stirring component and impeller are driven to move synchronously. In this way, while stirring the fermentation liquid in the fermenter, the fermentation liquid can also be continuously flowed through the sampling valve, thereby avoiding the problem of insufficient sample authenticity and representativeness caused by liquid stagnation. By setting up the ventilation component, in cooperation with the stirring component, it not only plays the role of transporting air, but also ensures that the pressure in the fermenter is kept stable, thereby improving the fermentation efficiency. Attached Figure Description

[0019] To more clearly illustrate the technical solutions in the embodiments of the present invention, the accompanying drawings used in the description of the embodiments of the present invention will be briefly introduced below. Obviously, the drawings described below are merely some exemplary embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on the content of the exemplary embodiments of the present invention and these drawings without any creative effort.

[0020] Figure 1 This is a schematic diagram of the overall structure of the present invention; Figure 2 This is a three-dimensional structural schematic diagram in cross-section of the present invention; Figure 3 This is a three-dimensional structural schematic diagram viewed from another angle in this invention; Figure 4 For the present invention Figure 2 A magnified schematic diagram of the partial structure at point A in the middle; Figure 5 For the present invention Figure 2 A magnified schematic diagram of the local structure at point B; Figure 6 For the present invention Figure 3 A magnified schematic diagram of the structure at point C in the middle; Figure 7 This is a schematic diagram of the stirring assembly in this invention.

[0021] In the diagram: 1. Fermentation tank; 2. Fixed frame; 3. Feed inlet; 4. Discharge pipe; 5. Control valve; 6. Stirring shaft; 7. Sampling cylinder; 8. Impeller; 9. Liquid inlet pipe; 10. Sampling valve; 11. First connecting pipe; 12. Second connecting pipe; 13. Sampling bottle; 14. Bottle cap; 15. Mounting frame; 16. Driving pulley; 17. Driven pulley; 18. Transmission belt; 19. Motor; 20. Transmission shaft; 21. Mounting cylinder; 22. Air pump; 23. Air pipe; 24. Exhaust valve; 25. Hemispherical shell; 26. Guide groove; 27. Stirring frame; 28. Stirring plate; 29. ​​Sealing cap. Detailed Implementation

[0022] The present invention will now be described in further detail with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and not intended to limit it.

[0023] To keep the drawings concise, each drawing only schematically shows the parts relevant to the invention; these do not represent the actual structure of the product. Furthermore, for ease of understanding, in some drawings, only one of components with the same structure or function is schematically shown, or only one is labeled. In this document, "one" not only means "only one," but can also mean "more than one," and "several" includes "two" and "more than two."

[0024] In this document, it should be noted that, unless otherwise explicitly specified and limited, the terms "installation," "connection," and "linking" should be interpreted broadly. For example, they can refer to fixed connections, detachable connections, or integral connections; they can refer to mechanical connections or electrical connections; they can refer to direct connections or indirect connections through an intermediate medium; and they can refer to the internal communication between two components. Those skilled in the art can understand the specific meaning of the above terms in this invention based on the specific circumstances.

[0025] In this invention, unless otherwise explicitly specified and limited, "above" or "below" the second feature can include direct contact between the first and second features, or contact between the first and second features through another feature between them. Furthermore, "above," "over," and "on top" of the second feature includes the first feature directly above or diagonally above the second feature, or simply indicates that the first feature is at a higher horizontal level than the second feature. "Below," "below," and "under" the second feature includes the first feature directly below or diagonally below the second feature, or simply indicates that the first feature is at a lower horizontal level than the second feature.

[0026] In the description of this embodiment, terms such as "upper," "lower," "left," and "right" are based on the orientation or positional relationship shown in the accompanying drawings. They are used only for the convenience of description and simplification of operation, and are not intended to indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on the present invention.

[0027] Furthermore, in the description of this application, the terms "first," "second," etc., are used only to distinguish descriptions and should not be construed as indicating or implying relative importance.

[0028] Example 1, as Figures 1 to 7As shown, this embodiment proposes a vitamin K2 extraction device, including a fermenter 1, which is fixedly mounted on a fixed frame 2. A feed inlet 3 is installed on the top of the fermenter 1, and a sealing cap 29 is provided on the feed inlet 3. A discharge pipe 4 is connected to the output end of the fermenter 1, and a control valve 5 is fixedly installed on the discharge pipe 4. The device also includes a stirring shaft 6, a sampling cylinder 7, a sampling assembly, a drive assembly, a ventilation assembly, and a stirring assembly. The stirring shaft 6 is rotatably mounted inside the fermenter 1. The sampling cylinder 7 is fixedly mounted on the side of the fermenter 1, and the sampling assembly is mounted on the sampling cylinder 7. The fermentation broth is sampled during the fermentation process. The sampling assembly includes an impeller 8, an inlet pipe 9, a sampling valve 10, and a sampling section. The impeller 8 is coaxially rotatably installed inside the sampling cylinder 7. One end of the inlet pipe 9 is connected to the upper part of the sampling cylinder 7, and the other end of the inlet pipe 9 is connected to the side wall of the fermenter 1. The sampling valve 10 is installed at the bottom of the sampling cylinder 7. One end of the sampling valve 10 is connected to the lower part of the sampling cylinder 7 through a first connecting pipe 11, and the other end of the sampling valve 10 is connected to the side wall of the fermenter 1 through a second connecting pipe 12. The sampling section is installed at the bottom of the sampling valve 10 for collecting samples.

[0029] Specifically, the production of vitamin K2 requires fermentation. First, the inside of fermentation tank 1 is sterilized at high temperature. Then, the inoculum is introduced for inoculation. After inoculation, the ventilation system is turned on to continuously introduce air into fermentation tank 1 and maintain a certain pressure and temperature inside fermentation tank 1 to cultivate the inoculum (during this period, the temperature and dissolved oxygen are automatically controlled by the supporting automatic control system). After more than 100 hours of cultivation, the cultivation is completed.

[0030] During fermentation, the drive assembly is activated to rotate the stirring shaft 6, thereby driving the stirring assembly to agitate the fermentation broth. This ensures that the cell particles and nutrients are evenly distributed, and also maintains a uniform temperature in the fermentation broth. During fermentation, multiple samples of the fermentation broth in fermenter 1 are taken and analyzed to determine the fermentation status. When sampling, since the liquid level of the fermentation broth is above the inlet pipe 9, the sampling cylinder 7, inlet pipe 9, sampling valve 10, first connecting pipe 11, and second connecting pipe 12 are all filled with fermentation broth. The drive assembly's configuration allows for efficient agitation. During the process of shaft 6, the impeller 8 is driven to rotate. As the impeller 8 rotates, it provides a downward thrust to the fermentation liquid in the sampling cylinder 7. At this time, the liquid in the sampling cylinder 7, the inlet pipe 9, the sampling valve 10, the first connecting pipe 11, and the second connecting pipe 12 flows. The flow direction is that the fermentation liquid in the fermenter 1 enters the sampling cylinder 7 through the inlet pipe 9, and then passes through the first connecting pipe 11, the sampling valve 10, and the second connecting pipe 12 in sequence, and finally returns to the fermenter 1, forming a cycle. This ensures that when sampling is performed using the sampling section, the sample taken is more representative of the overall fermentation situation of the fermentation liquid in the fermenter 1, so that the test results are closer to the actual fermentation situation.

[0031] After the fermentation broth has been cultured, it will be directly concentrated (that is, the control valve 5 will be opened to send the fermentation broth into the concentrator through the discharge pipe 4 for concentration). When the fermentation broth is concentrated to a certain concentration, it will be dried and powdered through a spray drying tower. (The top of the drying tower has a high-speed rotating atomizer, which atomizes the concentrated fermentation broth and sprays it into the drying tower. The top side of the drying tower has a hot air device to dry the sprayed fermentation broth, evaporate its moisture, and make it into a powdered product). The bottom of the drying tower is the discharge port. The finished powder is collected by a cyclone separator after the discharge port. The separated exhaust gas is treated and then discharged into the atmosphere.

[0032] like Figure 1 , Figure 2 , Figure 5 As shown above, the sampling unit includes a sampling bottle 13 and a bottle cap 14. The sampling bottle 13 is provided at the output end of the sampling valve 10, and the bottle cap 14 is installed at the mouth of the sampling bottle 13. The bottle cap 14 is connected to the output end of the sampling valve 10.

[0033] Specifically, when taking samples, the sampling bottle 13 is installed at the bottom of the bottle cap 14, and then the sampling valve 10 is opened to sample the fermentation liquid. After taking out a certain amount of fermentation liquid, the sampling valve 10 is closed, and the sampling bottle 13 is removed from the bottom of the sampling valve 10. The fermentation liquid can then be sent to the testing stage for analysis.

[0034] likeFigures 1 to 4 As shown, the drive assembly is installed on the top of the fermenter 1 to drive the stirring shaft 6 and the sampling assembly. The drive assembly includes a mounting frame 15, a drive pulley 16, a driven pulley 17, a transmission belt 18, a motor 19, and a transmission unit. The mounting frame 15 is fixedly installed on the top of the fermenter 1. The drive pulley 16 and the driven pulley 17 are rotatably installed inside the mounting frame 15. The driven pulley 17 is coaxially and fixedly connected to the top of the stirring shaft 6. The transmission belt 18 is tensioned and fitted between the drive pulley 16 and the driven pulley 17. The motor 19 is fixedly installed on the top of the mounting frame 15. The output end of the motor 19 is coaxially and fixedly connected to the drive pulley 16. The transmission unit is installed at the bottom of the mounting frame 15 to drive the impeller 8 to rotate.

[0035] Specifically, when it is necessary to drive the stirring shaft 6 to rotate, the motor 19 is turned on. The motor 19 drives the drive pulley 16 to rotate. The drive pulley 16 drives the driven pulley 17 to rotate through the transmission belt 18. Thus, the driven pulley 17 drives the stirring shaft 6 to rotate, which can stir the fermentation liquid in the fermentation tank 1. At the same time, through the transmission unit, the impeller 8 is driven to rotate synchronously.

[0036] like Figure 2 , Figure 4 As shown above, the transmission unit includes a transmission shaft 20 and a mounting cylinder 21. The top end of the transmission shaft 20 is coaxially and fixedly installed at the bottom of the drive pulley 16. The bottom end of the transmission shaft 20 is coaxially and fixedly connected to the impeller 8. The mounting cylinder 21 is rotatably installed on the outside of the transmission shaft 20. The mounting cylinder 21 is fixedly installed between the mounting frame 15 and the sampling cylinder 7.

[0037] Specifically, as the drive pulley 16 rotates, it drives the transmission shaft 20 to rotate inside the mounting cylinder 21, thereby driving the impeller 8 to rotate.

[0038] like Figure 1 , Figure 2 , Figure 3 , Figure 6 As shown, the ventilation assembly is installed on the top of the fermentation tank 1 to maintain air supply during the fermentation process. The ventilation assembly includes an air pump 22, an exhaust valve 24, and an air guide. An air pipe 23 is connected between the output end of the air pump 22 and the top of the fermentation tank 1. The exhaust valve 24 is connected to the top of the fermentation tank 1. The air guide is located at the output end of the air pipe 23 and is installed on the inner top wall of the fermentation tank 1 to guide the air entering the fermentation tank 1.

[0039] Specifically, during the fermentation process, air needs to be continuously supplied into the fermentation tank 1. Before supplying air, an air filtration device is connected to the input end of the air pump 22 to treat the air entering the air pump 22, thereby removing various impurities in the air and ensuring the cleanliness of the air. Then, the air pump 22 is turned on, and the air pump 22 sends air into the air pipe 23. Through the setting of the air pipe 23, air is continuously supplied into the fermentation tank 1 to provide sufficient oxygen for the fermentation process. Through the setting of the air guide, the air entering the fermentation tank 1 is prevented from directly blowing into the fermentation liquid. By adjusting the opening pressure of the exhaust valve 24, when a certain pressure is reached in the fermentation tank 1, the exhaust valve 24 can be opened to discharge excess air and maintain the pressure stability inside the fermentation tank 1.

[0040] like Figure 6 As shown above, the air guide section includes a hemispherical shell 25 and guide grooves 26. The hemispherical shell 25 is located at the output end of the air pipe 23. The hemispherical shell 25 is fixedly installed on the inner top wall of the fermenter 1. Several guide grooves 26 are opened at equal angles in a circular shape on the hemispherical shell 25.

[0041] Specifically, when air enters the fermenter 1 through the air pipe 23, the air is blocked by the hemispherical shell 25, thereby dispersing the concentrated airflow, and then discharged into the fermenter 1 through the guide groove 26.

[0042] like Figure 3 , Figure 7 As shown, a stirring assembly is installed on the stirring shaft 6 for stirring the fermentation broth. The stirring assembly includes a stirring frame 27 and a stirring plate 28. Two stirring frames 27 are symmetrically and fixedly installed on the stirring shaft 6. There is a gap between the side of each stirring frame 27 and the inner wall of the fermentation tank 1. The side of the stirring frame 27 closest to the inner wall of the fermentation tank 1 is set as an inclined surface. Several stirring plates 28 are fixedly installed at equal intervals inside the two stirring frames 27. Each stirring plate 28 is arranged at an angle.

[0043] Specifically, as the stirring shaft 6 rotates, the stirring shaft 6 drives the stirring frame 27 and the stirring plate 28 to rotate when stirring the fermentation liquid. Since the side of the stirring frame 27 closest to the inner wall of the fermentation tank 1 is set as an inclined surface, as the stirring proceeds, when the fermentation liquid passes through the side of the stirring frame 27, it will be affected by the inclined surface and generate eddies, thereby improving the uniformity of stirring.

[0044] The working principle or usage process of this application is as follows: In the production of vitamin K2, fermentation is required first. The inside of fermentation tank 1 is first sterilized at high temperature. Then, the inoculum is introduced through inlet 3 for inoculation. After inoculation, the sealing cap 29 is placed on inlet 3 to seal it. Then, the air pump 22 is turned on, sending air into the air pipe 23. Through the air pipe 23, air is continuously supplied into fermentation tank 1, providing sufficient oxygen for the fermentation process. When air enters fermentation tank 1 through air pipe 23, it is blocked by the hemispherical shell 25, thus dispersing the concentrated airflow. Then, it is discharged into fermentation tank 1 through guide groove 26. By adjusting the opening pressure of exhaust valve 24, when a certain pressure is reached inside fermentation tank 1, the exhaust valve 24 is opened to discharge excess air, maintaining stable pressure inside fermentation tank 1. After more than one hundred hours of cultivation, the cultivation is complete.

[0045] During fermentation, the motor 19 is turned on, which drives the drive pulley 16 to rotate. The drive pulley 16, through the transmission belt 18, drives the driven pulley 17 to rotate, which in turn drives the stirring shaft 6 to rotate. As the stirring shaft 6 rotates, it stirs the fermentation liquid, causing the stirring frame 27 and stirring plate 28 to rotate, ensuring that the cell particles and nutrients are always evenly distributed, and also ensuring that the temperature of the fermentation liquid is uniform.

[0046] During fermentation, the fermentation liquid in fermenter 1 needs to be sampled multiple times and then tested to determine the fermentation status. During sampling, since the liquid level of the fermentation liquid is above the inlet pipe 9, the sampling cylinder 7, inlet pipe 9, sampling valve 10, first connecting pipe 11, and second connecting pipe 12 are all filled with fermentation liquid. As the drive pulley 16 rotates, it drives the transmission shaft 20 to rotate within the mounting cylinder 21, thereby driving the impeller 8 to rotate. The rotation of the impeller 8 provides a downward thrust to the fermentation liquid in the sampling cylinder 7, causing the liquid in the sampling cylinder 7, inlet pipe 9, sampling valve 10, first connecting pipe 11, and second connecting pipe 12 to flow. The flow direction is as follows: the fermentation liquid in the fermenter 1 enters the sampling cylinder 7 through the inlet pipe 9, then passes through the first connecting pipe 11, the sampling valve 10, and the second connecting pipe 12 in sequence, and finally returns to the fermenter 1, forming a cycle. When sampling, the sampling bottle 13 is installed at the bottom of the bottle cap 14, and then the sampling valve 10 is opened to sample the fermentation liquid. After a certain amount of fermentation liquid is taken out, the sampling valve 10 is closed, and the sampling bottle 13 is removed from the bottom of the sampling valve 10. The fermentation liquid can then be sent to the testing stage for analysis. This ensures that when using the sampling unit to take samples, the samples taken are more representative of the overall fermentation situation of the fermentation liquid in the fermenter 1, so that the test results are closer to the actual fermentation situation.

[0047] After the fermentation broth has been cultured, it will be directly concentrated (that is, the control valve 5 will be opened to send the fermentation broth into the concentrator through the discharge pipe 4 for concentration). When the fermentation broth is concentrated to a certain concentration, it will be dried and powdered through a spray drying tower. (The top of the drying tower has a high-speed rotating atomizer, which atomizes the concentrated fermentation broth and sprays it into the drying tower. The top side of the drying tower has a hot air device to dry the sprayed fermentation broth, evaporate its moisture, and make it into a powdered product). The bottom of the drying tower is the discharge port. The finished powder is collected by a cyclone separator after the discharge port. The separated exhaust gas is treated and then discharged into the atmosphere.

[0048] Example 2: Based on a vitamin K2 extraction device, this example also proposes a vitamin K2 extraction method, including the following steps: The first step is inoculation. The inside of the fermenter 1 is sterilized at high temperature. Then, the inoculum is added through the feed inlet 3 for inoculation. After inoculation, the sealing cap 29 is placed on the feed inlet 3 to seal it.

[0049] The second step is ventilation. The air pump 22 is turned on, and the air pump 22 sends air into the air pipe 23. Through the setting of the air pipe 23, the air is continuously sent into the fermentation tank 1 to provide sufficient oxygen for the fermentation process. When the air enters the fermentation tank 1 through the air pipe 23, the air will be blocked by the hemispherical shell 25, thereby dispersing the concentrated airflow. Then, through the setting of the guide groove 26, it is discharged into the fermentation tank 1.

[0050] The third step is to regulate the air pressure by adjusting the opening pressure of the exhaust valve 24. When the pressure inside the fermentation tank 1 reaches a certain level, the exhaust valve 24 can be opened to release excess air and maintain the pressure inside the fermentation tank 1.

[0051] The fourth step is stirring. The motor 19 is turned on, and the motor 19 drives the drive pulley 16 to rotate. The drive pulley 16 drives the driven pulley 17 to rotate through the transmission belt 18. In turn, the driven pulley 17 drives the stirring shaft 6 to rotate. As the stirring shaft 6 rotates, it stirs the fermentation liquid. The stirring shaft 6 will drive the stirring frame 27 and the stirring plate 28 to rotate, so that the bacterial particles and nutrients are always evenly distributed. At the same time, it also plays a role in uniformly distributing the temperature of the fermentation liquid.

[0052] The fifth step is to push. As the drive pulley 16 rotates, it drives the transmission shaft 20 to rotate inside the mounting cylinder 21, thereby driving the impeller 8 to rotate. As the impeller 8 rotates, it provides a downward thrust to the fermentation liquid in the sampling cylinder 7. At this time, the liquid in the sampling cylinder 7, the inlet pipe 9, the sampling valve 10, the first connecting pipe 11, and the second connecting pipe 12 flows.

[0053] The sixth step is circulation. The fermentation liquid in the fermenter 1 enters the sampling tube 7 through the inlet pipe 9, and then passes through the first connecting pipe 11, the sampling valve 10 and the second connecting pipe 12 in sequence, and finally returns to the fermenter 1 to form a cycle.

[0054] Step 7: Sampling. Install the sampling bottle 13 at the bottom of the bottle cap 14, and then open the sampling valve 10 to sample the fermentation broth. After taking out a certain amount of fermentation broth, close the sampling valve 10 and remove the sampling bottle 13 from the bottom of the sampling valve 10. The fermentation broth can then be sent to the testing stage for analysis. This ensures that when using the sampling unit to take samples, the samples taken are more representative of the overall fermentation status of the fermentation broth in the fermenter 1, so that the test results are closer to the actual fermentation situation. The cultivation is completed after more than 100 hours of cultivation.

[0055] Step 8: Fermentation. The fermentation broth that has been cultured will be directly concentrated (that is, the control valve 5 will be opened to send the fermentation broth into the concentrator through the discharge pipe 4 for concentration).

[0056] Step 9: Powdering. After the fermentation liquid is concentrated to a certain concentration, it is dried and collected into powder through a spray drying tower. (The top of the drying tower has a high-speed rotating atomizer that atomizes the concentrated fermentation liquid and sprays it into the drying tower. The top side of the drying tower has a hot air device to dry the sprayed fermentation liquid, evaporating its moisture and turning it into a powdered product.) The bottom of the drying tower is the discharge port. The finished powder is then collected by a cyclone separator. The separated exhaust gas is treated and then discharged into the atmosphere.

[0057] It should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit it. Although the present invention has been described in detail with reference to preferred embodiments, those skilled in the art should understand that modifications or equivalent substitutions can be made to the technical solutions of the present invention without departing from the spirit and scope of the technical solutions of the present invention, and all such modifications or substitutions should be covered within the scope of the claims of the present invention.

Claims

1. A vitamin K2 extraction device, comprising a fermentation tank (1) fixedly installed on a fixed rack (2), a feed inlet (3) being installed on the top of the fermentation tank (1), a discharge pipe (4) being communicated with the output end of the fermentation tank (1), and a control valve (5) being fixedly installed on the discharge pipe (4), characterized in that, Also include: Stirring shaft (6), the stirring shaft (6) is rotatably mounted inside the fermentation tank (1); Sampling cylinder (7), the sampling cylinder (7) is fixedly installed on the side of the fermentation tank (1); Sampling assembly, the sampling assembly is installed on the sampling cylinder (7), for sampling the fermentation broth during fermentation; Driving assembly, the driving assembly is installed on the top of the fermentation tank (1), for driving the stirring shaft (6) and the sampling assembly; Ventilation assembly, the ventilation assembly is installed on the top of the fermentation tank (1), for maintaining the air inlet during fermentation; Stirring assembly, the stirring shaft (6) is installed on the stirring assembly, for stirring the fermentation broth.

2. The vitamin K2 extraction apparatus according to claim 1, characterized by The sampling assembly comprises: Impeller (8), the impeller (8) is coaxially rotatably installed inside the sampling cylinder (7); Liquid inlet pipe (9), one end of the liquid inlet pipe (9) is communicated on the upper part of the sampling cylinder (7), and the other end of the liquid inlet pipe (9) is communicated on the side wall of the fermentation tank (1); Sampling valve (10), the sampling valve (10) is arranged on the bottom of the sampling cylinder (7), one end of the sampling valve (10) is communicated with the lower part of the sampling cylinder (7) through the first connecting pipe (11), and the other end of the sampling valve (10) is communicated with the side wall of the fermentation tank (1) through the second connecting pipe (12); Sampling part, the sampling part is installed on the bottom of the sampling valve (10), for collecting samples.

3. The vitamin K2 extraction apparatus of claim 2, wherein The sampling part comprises: Sampling bottle (13), the output end of the sampling valve (10) is provided with the sampling bottle (13); Bottle cap (14), the bottle cap (14) is installed on the bottle mouth of the sampling bottle (13), and the bottle cap (14) is communicated with the output end of the sampling valve (10).

4. The vitamin K2 extraction apparatus according to claim 3, wherein The driving assembly comprises: Mounting rack (15), the mounting rack (15) is fixedly installed on the top of the fermentation tank (1); Driving pulley (16) and driven pulley (17), the driving pulley (16) and the driven pulley (17) are rotatably installed inside the mounting rack (15), and the driven pulley (17) is coaxially fixedly connected with the top of the stirring shaft (6); Transmission belt (18), the transmission belt (18) is tightly sleeved between the driving pulley (16) and the driven pulley (17); Motor (19), the motor (19) is fixedly installed on the top of the mounting rack (15), and the output end of the motor (19) is coaxially fixedly connected with the driving pulley (16); Transmission part, the transmission part is installed on the bottom of the mounting rack (15), for driving the impeller (8) to rotate.

5. A vitamin K2 extraction device according to claim 4, characterized in that The transmission part comprises: Transmission shaft (20), the top end of the transmission shaft (20) is coaxially and fixedly installed on the bottom of the driving pulley (16), and the bottom end of the transmission shaft (20) is coaxially fixedly connected with the impeller (8); Mounting cylinder (21), the mounting cylinder (21) is rotatably installed outside the transmission shaft (20), and the mounting cylinder (21) is fixedly installed between the mounting rack (15) and the sampling cylinder (7).

6. A vitamin K2 extraction device according to claim 5, characterized in that The ventilation assembly comprises: A gas pump (22) is communicated with the top of the fermentation tank (1) through a gas pipe (23); An exhaust valve (24) is communicated with the top of the fermentation tank (1); An air guide part is arranged at the output end of the gas pipe (23), and the air guide part is installed on the inner top wall of the fermentation tank (1) and used for guiding the air entering the fermentation tank (1).

7. A vitamin K2 extraction device according to claim 6, characterized in that The air guide part comprises: A hemispherical shell (25) is arranged at the output end of the gas pipe (23), and the hemispherical shell (25) is fixedly installed on the inner top wall of the fermentation tank (1); A plurality of guide grooves (26) are circumferentially and equally angularly arranged on the hemispherical shell (25).

8. The vitamin K2 extraction device of claim 7, wherein, The stirring assembly comprises: Two stirring frames (27) are symmetrically and fixedly installed on the stirring shaft (6), and gaps exist between the side portions of the two stirring frames (27) and the inner side wall of the fermentation tank (1); A plurality of stirring plates (28) are fixedly installed at equal intervals in the two stirring frames (27), and each stirring plate (28) is arranged obliquely.

9. A vitamin K2 extraction device according to claim 8, characterized in that The side of the stirring frame (27) close to the inner side wall of the fermentation tank (1) is arranged as an inclined surface.

10. A method for extracting vitamin K2 using the vitamin K2 extraction apparatus according to any one of claims 1 to 9, characterized by, The method comprises the following steps: In a first step, inoculation, the inside of the fermentation tank (1) is sterilized at high temperature, and then the strain is inoculated by being put into the fermentation tank (1) through the feeding port (3); In a second step, aeration, the gas pump (22) is started, and the gas pump (22) sends air into the gas pipe (23), and the air is continuously sent into the inside of the fermentation tank (1) through the arrangement of the gas pipe (23); In a third step, air pressure adjustment, the opening pressure of the exhaust valve (24) is adjusted, and when the pressure in the fermentation tank (1) reaches a certain value, the exhaust valve (24) is opened to discharge the excess air; In a fourth step, stirring, the motor (19) is started, the motor (19) drives the driving pulley (16) to rotate, the driving pulley (16) drives the driven pulley (17) to rotate through the arrangement of the transmission belt (18), so as to drive the stirring shaft (6) to rotate through the driven pulley (17), and the stirring shaft (6) drives the stirring frame (27) and the stirring plate (28) to rotate with the rotation of the stirring shaft (6), so as to stir the fermentation liquid; In a fifth step, pushing, the driving pulley (16) drives the impeller (8) to rotate through the transmission shaft (20) with the rotation of the driving pulley (16), so as to provide a downward pushing force for the fermentation liquid in the sampling cylinder (7); In a sixth step, circulation, the fermentation liquid in the fermentation tank (1) enters the inside of the sampling cylinder (7) through the liquid inlet pipe (9), and then sequentially passes through the first connecting pipe (11), the sampling valve (10) and the second connecting pipe (12), and finally returns to the inside of the fermentation tank (1), thereby forming a circulation. The seventh step is sampling. The sampling bottle (13) is installed at the bottom of the bottle cap (14), and then the sampling valve (10) is opened to sample the fermentation liquid. After a certain amount of fermentation liquid is taken out, the sampling valve (10) is closed, the sampling bottle (13) is removed from the bottom of the sampling valve (10), and the fermentation liquid is sent to the inspection link for testing until the culture is completed. The eighth step is concentration. The fermentation liquid after the culture is completed is directly concentrated. The ninth step is powder making. After the fermentation liquid is concentrated to a certain concentration, it is dried and collected into powder through a spray drying tower. The bottom of the drying tower is a discharge port. The finished powder is collected through a cyclone separator after the discharge port. The separated tail gas is treated and discharged into the atmosphere.

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