Anti-pollution type bacterial culture device

Abstract

The invention relates to the technical field of medical instruments, in particular to an anti-pollution type bacterial culture device, and aims to solve the problem that indirect pollution exists in an existing closed culture device. The anti-pollution type bacterial culture device comprises a box body, a transparent turning cover is arranged on the box body, and a tool disinfection chamber and a bacterial culture chamber are arranged in the box body; the device further comprises an operation area, a culture bottle control mechanism, a disinfection mechanism and an isolation mechanism. The operation area is arranged in the box body and is positioned between the tool disinfection chamber and the bacterial culture chamber; the culture bottle control mechanism is arranged in the bacterial culture room; the disinfection mechanism is arranged in the tool disinfection chamber; and the isolation mechanism is used for actively transferring the disinfected reagent bottles and operation tools to an operation area in a sterile manner. According to the anti-pollution type bacterial culture device, the three functional areas including the tool disinfection chamber, the operation area and the bacterial culture chamber can be physically isolated through the isolation mechanism, and whole-process closed-loop management of disinfection, transfer, operation and culture is achieved.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of medical devices, and particularly relates to a bacteria culture device with pollution prevention. BACKGROUND

[0002] Bacteria culture is one of the most basic and key experimental techniques in the fields of microbiology, medical examination, biological pharmacy, and environmental monitoring. In actual operation, bacteria need to proliferate in a specific temperature, humidity, and sterile environment to ensure the accuracy and repeatability of experimental results. Traditional bacteria culture usually relies on a constant temperature incubator combined with a super-clean workbench or a biological safety cabinet to complete inoculation, subculture, drug sensitivity test, and other operations. However, the existing technology exposes many problems in actual application, which seriously restricts the experimental efficiency and biological safety.

[0003] Most of the existing closed culture devices adopt a combination structure of "main container + auxiliary interface": the culture unit is usually a rigid container with a sealing cover, equipped with temperature and humidity control modules to meet the bacteria growth conditions; sampling and reagent adding operations are mainly realized through two types of interfaces: One type is an elastic self-sealing hole structure, such as the existing technology that uses an elastic rubber cover to open a self-sealing hole matched with a pipette tip, which relies on the material elasticity to keep closed without external force; the hole disinfection relies on manual alcohol cotton wiping or ultraviolet lamp irradiation, and the disinfection operation and subsequent sampling / reagent adding operation are independent of each other, if the manual disinfection is not thorough (such as missing the hole edge), or there is a time interval between disinfection and operation, foreign bacteria are easy to adhere to the interface surface and enter the culture system with operation.

[0004] The other type is a puncture type interface, such as The system's double-needle puncture device completes the operation by puncturing the sealing plug, although it can be sterilized by burning the metal needle with a flame, but the needle body needs to pass through the air to contact the sealing plug during the puncture process, and the plankton in the air may adhere to the surface of the needle body, and the needle body needs to be burned and sterilized again when the culture container is replaced, the interface is still exposed at the operation gap, increasing the probability of secondary pollution.

[0005] The disinfection area of most devices is an independent component (such as an ultraviolet disinfection lamp beside the interface, an alcohol cotton placement slot), which is not deeply integrated with the operation channel. For example, some closed culture devices only set a separate ultraviolet disinfection module on the top of the culture container, and the operation channel (such as the puncture hole, the elastic hole) has a certain distance from the disinfection area, so the disinfection light cannot completely cover the inside of the channel, and the inner wall of the channel is easy to leave foreign bacteria; and the sampling gun head and reagent dropping head need to pass through the operation channel during operation, if the inner wall of the channel is not completely disinfected, it will contaminate the gun head / dropping head, and then cause the culture system to be contaminated. In addition, the disinfection area cannot be automatically started according to the operation process, and needs to be manually started by the operator, if the operator forgets the disinfection step and directly performs the sampling / reagent adding operation, the disinfection protection function will be completely lost. Summary of the Invention

[0006] To address the problems existing in the prior art, the present invention provides a contamination-proof bacterial culture device. On the one hand, the isolation mechanism physically isolates the three functional areas of the tool disinfection room, the operation area, and the bacterial culture room, realizing closed-loop management of the entire process of disinfection-transfer-operation-culture. On the other hand, the sliding plate + telescopic plate + moving rod can achieve flexible support when the reagent bottle falls, with the telescopic plate being pressed back and the moving rod separating along the inclined plane.

[0007] To achieve the above objectives, the technical solution adopted by the present invention is as follows: This application provides a contamination-proof bacterial culture device, including a housing with a transparent flip-top. The housing contains a tool sterilization chamber and a bacterial culture chamber. It also includes an operating area, a culture bottle control mechanism, a sterilization mechanism, and an isolation mechanism. The operating area is located inside the housing, between the tool sterilization chamber and the bacterial culture chamber. The culture bottle control mechanism is located in the bacterial culture chamber and is used to adjust the position of the culture bottles. The sterilization mechanism is located in the tool sterilization chamber and is used to sterilize reagent bottles and operating tools. The isolation mechanism is located between the tool sterilization chamber and the operating area and is used to actively and aseptically transfer sterilized reagent bottles and operating tools to the operating area.

[0008] Furthermore, the operating area is equipped with clamps for holding culture flasks; the side wall of the operating area is provided with mounting holes for installing rubber gloves.

[0009] Furthermore, the isolation mechanism includes a sealed channel, a first sealed door, a second sealed door, and a sliding assembly; the sealed channel is inclined, with its rear end connected to the tool disinfection chamber and its front end connected to the operating area; the first sealed door is slidably connected to the rear end of the sealed channel and automatically opens when it comes into contact with reagent bottles and operating tools; the second sealed door is rotatably connected to the front end of the sealed channel; the sliding assembly is slidably disposed inside the sealed channel to support the operating tools and actively close the first sealed door.

[0010] Furthermore, a track is provided between the sealing door and the disinfection mechanism; a slider matching the track is provided at the rear end of the sealing door; a gravity member matching the track is movably provided at the top of the track, and the gravity member is used to press down the slider to move the sealing door.

[0011] Furthermore, the sliding assembly includes a sliding plate, a gravity member, and a buffer member; the rear end of the sliding plate is connected to the bottom of the sealed door via a rope, and the front and rear sides are connected to the inner wall of the sealed channel via ropes; the gravity member is connected to the end of the rope away from the sliding plate and is used to pull the sliding plate upward; the buffer member is slidably connected to the front end of the sliding plate and is used to protect the reagent bottle during transfer.

[0012] Furthermore, the buffer includes a second sliding plate, two telescopic plates, and two moving rods; the second sliding plate is slidably connected to the front end of the first sliding plate and is used to buffer the reagent bottle and operating tool when they fall; the two telescopic plates are slidably fitted onto the front end of the second sliding plate and have an inclined surface at the top; the two moving rods are slidably connected to the second sliding plate at the bottom and correspond to the inclined surface, so that the operating tool is released when the telescopic plates move backward and move away from each other.

[0013] Furthermore, the disinfection mechanism includes multiple rolling rods, a telescopic component, and a separation assembly; the multiple rolling rods are arranged side by side in the tool disinfection chamber for transporting reagent bottles and operating tools when rotating; the telescopic component is located below the rolling rods for adjusting the height of the rolling rods; the separation assembly is located on the left side of the rolling rods for separating the reagent bottles and operating tools.

[0014] Furthermore, the separation assembly includes a fixed plate, a partition, a displacement plate, and a pusher; the fixed plate is disposed in the tool sterilization chamber and located to the left of the rolling rod, and has an opening; the partition is slidably disposed in the tool sterilization chamber to separate the reagent bottle and the operating tool; the displacement plate is slidably disposed on the fixed plate to open and close the opening; one end of the pusher is connected to the partition and the other end is connected to the displacement plate, and it is used to simultaneously push the partition and the displacement plate.

[0015] Furthermore, the culture bottle control mechanism includes a mounting plate, a sealing door, and an auxiliary push rod; the mounting plate is rotatably connected to the bacterial culture chamber and has multiple mounting slots for placing culture bottles; the sealing door is slidably connected to the rear side of the bacterial culture chamber; the auxiliary push rod is set on the mounting plate, with its end corresponding to the mounting slot, and is used to push the culture bottle onto the clamp.

[0016] Furthermore, the box is equipped with multiple disinfection lamps.

[0017] Compared with the prior art, the beneficial effects of the present invention are: 1. By physically isolating the tool sterilization room, operating area, and bacterial culture room through an isolation mechanism, a closed-loop management system for the entire process of sterilization-transfer-operation-culture is achieved. This avoids air disturbance and contaminant intrusion caused by frequent opening and closing operations in traditional laminar flow hoods or ordinary incubators, reducing the risk of contamination caused by human error, improving the reproducibility and accuracy of experimental results, effectively preventing cross-contamination and external contamination, and significantly enhancing the safety, stability, and operational efficiency of bacterial culture. After sterilization, a gravity component presses down on the slider, causing the sealing door to open. The sliding component (slide plate one) slides upward along the inclined channel under the pull of gravity component two, carrying the sterilized tools / reagent bottles. Upon reaching the front end, it triggers the opening of the sealing door two, and the buffer component releases the items into the operating area. The entire process requires no manual intervention, avoiding the introduction of contaminants by hands or airflow, achieving aseptic transfer. Furthermore, by isolating the operating area and tool sterilization area throughout the process, it further prevents bacteria that may be present in the tool sterilization area from entering the operating area.

[0018] 2. The combination of a sliding plate, telescopic plate, and moving rod allows for flexible support when reagent bottles fall. The telescopic plate retracts under pressure, and the moving rod separates along the inclined plane, effectively absorbing the impact of reagent bottles or tools falling from a height. This prevents glassware breakage, extends equipment lifespan, reduces consumable consumption, and improves operational safety, making it particularly suitable for handling high-risk samples such as pathogens. Simultaneously, the sliding plate's descent closes the sealing door via a rope, automatically preventing air from entering the operating area from the tool sterilization zone as the tool enters the sealed channel. Furthermore, the sealing door automatically closes after the tool enters the operating area, further protecting the sterile environment of the operating area.

[0019] 3. The disinfection mechanism uses multiple parallel rolling rods to rotate and transport reagent bottles and tools of different sizes. Compared with static placement, dynamic transport greatly improves disinfection efficiency and coverage. The rolling mechanism can also flip the tools so that all sides of the tools can be in the disinfection environment, and multiple sides of the rolling rods can also be disinfected. At the same time, the separation component can automatically distinguish between reagent bottles and operating tools, avoiding mixing and preventing collisions between tools and reagent bottles, thus avoiding accidental bumps to the reagent bottles.

[0020] 4. The side wall of the operating area is equipped with mounting holes for securing rubber gloves. The box is equipped with a transparent flip cover for easy observation of the internal condition. Operators can perform delicate operations (such as inoculation and sampling) without disrupting the sealed environment, enabling operation in a sterile environment without the need for frequent disinfection or tool replacement, thus improving efficiency. Attached Figure Description

[0021] To more clearly illustrate the technical solutions of the embodiments of this application, the accompanying drawings used in the embodiments will be briefly introduced below. It should be understood that the following drawings only show some embodiments of this application and should not be regarded as a limitation of the scope. For those skilled in the art, other related drawings can be obtained based on these drawings without creative effort.

[0022] Figure 1 This is a schematic diagram of the overall structure of the present invention; Figure 2 This is a schematic diagram of the internal structure of the present invention; Figure 3 This is a top view of the internal structure of the present invention; Figure 4 This is a cross-sectional view of the tool disinfection chamber in this invention; Figure 5 For the present invention Figure 2 Enlarged view of point A in the middle; Figure 6 This is a schematic diagram of the structure of the spacer component in this invention; Figure 7 This is a schematic diagram of the structure of the pusher in this invention; Figure 8 For the present invention Figure 7 Enlarged view of point C in the middle; Figure 9 This is a schematic diagram of the structure of the push block in this invention; Figure 10 This is a schematic diagram of the installation disk in this invention; Figure 11 This is a schematic diagram of the structure of the sealing door three in this invention; Figure 12 This is a schematic diagram of the auxiliary rod in this invention; Figure 13 This is a schematic diagram of the structure of the rolling rod in this invention; Figure 14 This is a schematic diagram of the isolation mechanism in this invention; Figure 15 This is a cross-sectional view of the isolation mechanism of the present invention; Figure 16 For the present invention Figure 15 Enlarged view of point B in the middle; Figure 17 This is a schematic diagram of the sliding component in this invention; Figure 18 This is a schematic diagram of the buffer component in this invention.

[0023] In the diagram: 1-Box body; 11-Tool disinfection chamber; 111-Mounting plate; 12-Bacterial culture chamber; 121-Opening; 13-Operating area; 131-Placement slot; 132-Reset rod; 133-Moving pulley; 14-Mounting hole; 15-Flip cover; 2-Culture bottle control mechanism; 21-Mounting plate; 211-Mounting slot; 22-Sealing door three; 221-Electric push rod one; 23-Auxiliary push rod; 24-Auxiliary rod; 25-Gear one; 26-Motor one; 27-Gear two; 3-Disinfection mechanism; 31-Rolling rod; 32-Telescopic component; 33-Separation assembly; 331-Fixing plate; 332-Separator; 3321-Adapter slot; 333-Displacement plate; 334-Pushing component; 3341-Guide rod; 3342-Displacement rod; 3343-Driven rod; 3344-Push block; 335-Electric push rod II; 336-Leaving block; 34-Transmission pulley; 35-Motor II; 4-Isolation mechanism; 41-Sealed channel; 411-Accommodation channel; 412-Mounting shaft; 42-Sealed door I; 421-Slider; 43-Sealed door II; 44-Sliding assembly; 441-Slide plate I; 442-Gravity component II; 443-Buffer component; 4431-Slide plate II; 4432-Telescopic plate; 4433-Moving rod; 4434-Telescopic rod; 4435-Spring I; 4436-Spring II; 444-Rope I; 445-Rope II; 45-Rail; 46-Gravity component I; 5-Clamp; 6-Disinfection lamp. Detailed Implementation

[0024] The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments, and not all embodiments.

[0025] In the description of this invention, it should be understood that the terms "front", "rear", "left", "right", "upper", "lower", "top", "bottom", "inner", "outer", etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are only for the convenience of describing this invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this invention.

[0026] In the description of this application, it should also be noted that, unless otherwise expressly specified and limited, the terms "set up," "install," "connect," and "link" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a direct connection or an indirect connection through an intermediate medium; and they can refer to the internal connection of two components. Those skilled in the art can understand the specific meaning of the above terms in this application based on the specific circumstances. Example 1

[0027] Combination Figures 1-18 As shown, the present invention provides a contamination-resistant bacterial culture device, including a housing 1 with a transparent flip-top 15. The housing 1 contains a tool disinfection chamber 11 and a bacterial culture chamber 12. It also includes an operating area 13, a culture bottle control mechanism 2, a disinfection mechanism 3, and an isolation mechanism 4. The operating area 13 is located inside the housing 1 and between the tool disinfection chamber 11 and the bacterial culture chamber 12. The culture bottle control mechanism 2 is located within the bacterial culture chamber 12 and is used to adjust the position of the culture bottles. The disinfection mechanism 3 is located within the tool disinfection chamber 11 and is used to disinfect reagent bottles and operating tools. The isolation mechanism 4 is located between the tool disinfection chamber 11 and the operating area 13 and is used to actively and aseptically transfer disinfected reagent bottles and operating tools to the operating area 13.

[0028] Specifically, the enclosure 1 has a rectangular structure with a sealed bottom and four sides to protect the internal structure and integrate the various subsystems. It is made of stainless steel. A flip-top 15, made of transparent PC board with anti-fog treatment, is installed on top of the enclosure 1. It provides a top access point and ensures visual transparency. Multiple manually openable windows are located on the flip-top 15, corresponding to the positions of the tool sterilization chamber 11, the operating area 13, and the bacterial culture chamber 12. The tool sterilization chamber 11 is located at the rear end of the enclosure 1, while the bacterial culture chamber 12 is located at the front end. Both the tool sterilization chamber 11 and the bacterial culture chamber 12 are separated by stainless steel plates, ensuring that each is sealed and independent. The purpose of the operating area 13 is to facilitate the addition of reagents and sampling by operators inside the chamber 1 without opening the flip-top 15. After the operation is completed, the culture bottle is returned to the bacterial culture chamber 12 through the internal structure of the chamber 1. At this time, the door of the bacterial culture chamber 12 is closed, and the window of the operating area 13 is opened to retrieve the reagent bottle and operating tools, effectively avoiding contamination during the operation. It should be noted that the bacterial culture chamber 12 maintains a constant temperature and uses heating elements and temperature control sensors to achieve a stable culture environment. The inner liner of the bacterial culture chamber 12 is made of food-grade stainless steel, and the outer insulation layer is made of polyurethane foam material, which can reduce heat loss.

[0029] Furthermore, the operating area 13 is provided with a clamp 5 for holding culture flasks; the side wall of the operating area 13 is provided with a mounting hole 14 for installing rubber gloves.

[0030] There are two clamps 5, symmetrically arranged. Each clamp has an arc-shaped gripping head at the front end and is connected to the tool sterilization chamber 11 via an electric push rod at the rear end. The gripping head can be moved back and forth to adjust the gripping position. The gripping head is equipped with a spring, which can automatically clamp culture bottles of different diameters to stabilize them for inoculation or other operations. Mounting holes 14 are paired on the left and right side walls of the chamber 1. Small rubber gloves are located inside the chamber 1 with their openings facing outwards, allowing operators to perform operations in a sterile environment. The glove cuffs are pressed into the circular mounting holes 14 on the side wall of the operating area 13 via a flange structure and sealed with O-rings. The gloves are made of medical-grade nitrile rubber or neoprene rubber, with a thickness ≥0.6 mm, resistant to chemical corrosion, and are standard five-finger glove shape.

[0031] Furthermore, the disinfection mechanism 3 includes multiple rolling rods 31, a telescopic component 32, and a separation component 33; the multiple rolling rods 31 are arranged side by side in the tool disinfection chamber 11 for transporting reagent bottles and operating tools when rotating; the telescopic component 32 is located below the rolling rods 31 for adjusting the height of the rolling rods 31; the separation component 33 is located on the left side of the rolling rods 31 for separating the reagent bottles and operating tools.

[0032] An installation plate 111 is located on the right side of the interior of the tool disinfection chamber 11. The bottom end of the installation plate 111 is connected to the bottom of the interior of the tool disinfection chamber 11 via a telescopic component 32. A transmission pulley 34 is located on the left side of the installation plate 111. The transmission pulley 34 includes a transmission belt and multiple rotating components sleeved within the transmission belt. The rotating components at the front and rear ends are driven by a second motor 35, and the remaining rotating components are rotatably connected to the left side of the installation plate 111 via connecting rods. Starting the second motor 35 causes the rotating components to rotate with the transmission belt. The right ends of multiple rolling rods 31 are mounted on the corresponding rotating components, allowing the multiple rolling rods 31 to rotate at a uniform speed with the rotating components. There are two telescopic components 32, symmetrically arranged at the bottom of the installation plate 111, used to support the installation plate 111 and to adjust the height and tilt of the multiple rolling rods 31.

[0033] Furthermore, the separating component 33 includes a fixed plate 331, a partition plate 332, a displacement plate 333, and a pusher 334; the fixed plate 331 is disposed in the tool sterilization chamber 11 and located to the left of the rolling rod 31, and has an opening 121; the partition plate 332 is slidably disposed in the tool sterilization chamber 11 to separate the reagent bottle and the operating tool; the displacement plate 333 is slidably disposed on the fixed plate 331 to open and close the opening 121; one end of the pusher 334 is connected to the partition plate 332, and the other end is connected to the displacement plate 333, for simultaneously pushing the partition plate 332 and the displacement plate 333.

[0034] A fixed plate 331 is vertically fixed to the left side of the rolling rod 31, dividing the tool sterilization chamber 11 into two chambers with rectangular openings 121. Two displacement plates 333 are symmetrically and horizontally slidably connected to the fixed plate 331 to control whether items can pass through. The bottom of the partition plate 332 is vertically slidably embedded in the guide rail inside the tool sterilization chamber 11, and can move laterally to block specific paths to achieve sorting.

[0035] It should be noted that the pushing component 334 includes a guide rod 3341, a displacement rod 3342, a driven rod 3343, and a pushing block 3344. The driven rod 3343 is vertically connected to the left side wall of the tool disinfection chamber 11 via an electric actuator 335. The right end of the driven rod 3343 is connected to the telescopic end of the electric actuator 335, and the left end is provided with a T-shaped pushing block 3344 for pushing the partition 332. The displacement rod 3342 has an L-shaped structure, with a slot at its top that matches the pushing block 3344, and a guide rod 3341 at its bottom for pushing the displacement plate 333. There are two guide rods 3341, and the left ends of both are hinged to the right ends of the displacement rods 3342. The right ends of both guide rods are hinged to moving blocks and are slidably connected to two sliding grooves on the inner bottom side of the tool disinfection chamber 11 via the moving blocks. Two grooves are formed at the bottom of the two displacement plates 333, and the length of the two grooves is less than the width of the opening 121 of the fixed plate 331. The bottom of the displacement plate 333 is connected to the moving block. The bottom of the displacement rod 3342 is slidably connected to the bottom of the tool disinfection chamber 11. When the telescopic end of the electric push rod 335 extends, the driven rod 3343 moves to the right, and the pushing block 3344 drives the displacement rod 3342 to move to the right. When the displacement rod 3342 moves to the right, the ends of the two guide rods 3341 move away from each other, thereby driving the two displacement plates 333 to move away from each other and open the opening 121. When the two moving blocks move to the limit, the telescopic end of the electric push rod 335 continues to extend. At this time, the pushing block 3344 will disengage from the slot of the displacement rod 3342 so that the driven rod 3343 continues to move. The bottom end of the partition 332 is slidably connected to the right end of the displacement rod 3342. A matching groove 3321, matching the push block 3344, is provided on the top side near the displacement rod 3342. When the push block 3344 disengages from the displacement rod 3342 and continues to move to the right, it enters the matching groove 3321 to continue pushing the partition 332. Furthermore, clearance blocks 336 are provided on both sides of the push block 3344, and clearance blocks 336 are also provided on both sides of the openings of the slot and the matching groove 3321. The ends of the clearance blocks 336 are arc-shaped and made of a material with a certain degree of plasticity, facilitating the disengagement or insertion of the push block 3344 when subjected to a large external force.

[0036] Furthermore, the culture bottle control mechanism 2 includes a mounting plate 21, a sealing door 3 22, and an auxiliary push rod 23; the mounting plate 21 is rotatably connected inside the bacterial culture chamber 12 and has multiple mounting slots 211 for placing culture bottles; the sealing door 3 22 is slidably connected to the rear side of the bacterial culture chamber 12; the auxiliary push rod 23 is set on the mounting plate 21, with its end corresponding to the mounting slot 211, and is used to push the culture bottle onto the clamp 5.

[0037] A gear 27 is located at the bottom of the mounting plate 21. A gear 25 is connected to one side of the gear 27. The gear 25 is driven by a motor 26, causing the mounting plate 21 to rotate. A sealing door 22 is slidably connected to the rear side of the bacterial culture chamber 12, used to open and close the doorway at the rear of the bacterial culture chamber 12. The auxiliary push rod 23 is straight, with one end being an electric push rod and the other end having a flexible clamp for fixing the culture bottle. The auxiliary rod 24 includes two electric push rods connected end to end, with a limit groove at the end near the doorway to assist the auxiliary push rod 23 in moving the culture bottle.

[0038] Furthermore, the housing 1 is equipped with multiple disinfection lamps 6. The disinfection lamps 6 are fixed to the top wall of the tool disinfection chamber 11, the top of the operating area 13, and the area around the culture chamber by clips or screws, emitting 254 nm wavelength UVC ultraviolet light to destroy microbial DNA / RNA.

[0039] The specific principle of this embodiment is as follows: In use, the culture bottle that needs to be added with reagents or sampled is rotated to the position corresponding to the doorway. The auxiliary push rod 23 is activated to push the culture bottle backward until it is fixed between the two clamps 5. The electric push rod 235 is activated to move the driven rod 3343 to the right, pushing the displacement rod 3342 and the partition 332 to the right. The ends of the two guide rods 3341 move away from each other, thereby driving the two displacement plates 333 to move away from each other and opening the doorway 121. When the two moving blocks move to their limits, the telescopic end of the electric push rod 235 continues to extend. At this time, the push block 3344 will disengage from the slot of the displacement rod 3342, allowing the driven rod 3343 to continue to move. The push block 3344 continues to push the partition 3342. 2. Move the partition 332 to the right so that it is inserted between the two rolling rods 31. At this time, the push block 3344 enters the fitting slot 3321. Then open the window above the tool disinfection chamber 11, place the reagent bottle and operating tools on the rolling rods 31 and on both sides of the partition 332, close the window, turn on the disinfection lamp 6 for disinfection, and at the same time start the motor 2 35 to rotate the multiple rolling rods 31. After disinfection, retract the electric push rod 2 335 to reset the partition 332. Then extend the telescopic part 32 on the rear side to raise the rear end of the mounting plate 111. At this time, the reagent bottle and operating tools roll forward and fall into the placement slot 131 of the operating area 13 by gravity after entering the isolation mechanism 4. The operator puts his hands into the rubber gloves to add reagents and take samples. After the reagent addition and sampling are completed, start the moving pulley 133 to move the reset rod 132 to the side of the culture bottle, and then start the auxiliary push rod 23 to retract and send the culture bottle back to the mounting slot 211. Example 2

[0040] Based on Example 1, Example 2 provides a specific structure for the isolation mechanism 4, which significantly improves the safety, stability and operational efficiency of bacterial culture.

[0041] Specifically, the isolation mechanism 4 includes a sealing channel 41, a first sealing door 42, a second sealing door 43, and a sliding assembly 44. The sealing channel 41 is inclined, with its rear end connected to the tool disinfection chamber 11 and its front end connected to the operating area 13. The first sealing door 42 is slidably connected to the rear end of the sealing channel 41 and automatically opens when it comes into contact with reagent bottles and operating tools. The upper end of the second sealing door 43 is rotatably connected to the top of the front end of the sealing channel 41 via a rotating shaft, and its lower part automatically returns to its closed position by a torsion spring. The sliding assembly 44 is slidably disposed inside the sealing channel 41 and is used to support operating tools and actively close the first sealing door 42.

[0042] The rear end of the sealed channel 41 is welded to the outlet of the tool disinfection chamber 11, and the front end is connected to the operating area 13, with an inclination angle of approximately 30°~45°. Sealed doors 42 and 43 remain closed before and during the disinfection of reagent bottles and operating tools, and will only open automatically after the disinfection is completed, and will close automatically after transfer.

[0043] Furthermore, a track 45 is provided between the sealing door 42 and the disinfection mechanism 3; a slider 421 matching the track 45 is provided at the rear end of the sealing door 42; a gravity member 46 matching the track 45 is movably provided at the top of the track 45, and the gravity member 46 is used to press down the slider 421 to move the sealing door 42.

[0044] The track 45 is fixed inside the tool sterilization chamber 11 at the front and below the opening. A groove is provided at the top of the track 45 for placing the gravity component 46. The front side of the groove has a ramp, and the rear side is close to the rolling rod 31, controlling the gravity component 46 on the track 45. The gravity component 46 will only move when subjected to external force. Therefore, when the reagent bottle and operating tools roll towards the sealed channel 41, they push the gravity component 46 to roll above the slider 421. Gravity then moves the sealing door 42 downwards, opening the opening of the sealed channel 41, allowing the reagent bottle and operating tools to enter the sealed channel 41.

[0045] Furthermore, the sliding assembly 44 includes a sliding plate 441, a gravity member 442, and a buffer member 443; the rear end of the sliding plate 441 is connected to the bottom of the sealing door 42 via a rope 444, and the front and rear sides are connected to the inner wall of the sealing channel 41 via ropes 445; the gravity member 442 is connected to the end of the rope 445 away from the sliding plate 441, and is used to pull the sliding plate 441 upward; the buffer member 443 is slidably connected to the front end of the sliding plate 441, and is used to protect the reagent bottle during transfer.

[0046] The two sides of the slide plate 441 are slidably connected to the two sides of the sealing channel 41. In the initial state, the slide plate 441 is located inside the rear side of the sealing channel 41. At this time, the rope 444 has sufficient slack inside the sealing door 42. When the sealing door 42 moves down, the rope 444 will be tightened. The top of the left and right sides of the sealing channel 41 is provided with receiving channels 411. The rear end of the receiving channel 411 is provided with a mounting shaft 412. The second rope 445 passes through the receiving channel 411 and rests on the mounting shaft 412. One end of the second rope 445 is connected to the side end of the slide plate 441, and the other end located inside the receiving channel 411 is connected to the second gravity member 442. Since the sealing channel 41 is inclined, the receiving channel 411 is also inclined. The second gravity member 442 is tightened by its own weight. The weight of the second gravity member 442 is greater than the weight of the slide plate 441, thereby pulling the slide plate 441 upward so that the slide plate 441 is located at the rear end of the sealing channel 41. When the sterilized reagent bottle and operating tools are both on the slide plate 441, the weight of the reagent bottle and operating tools plus the slide plate 441 is greater than the weight of the gravity component 442 plus the gravity component 46, which will cause the slide plate 441 to slide forward.

[0047] Furthermore, the buffer 443 includes a second sliding plate 4431, two telescopic plates 4432, and two moving rods 4433; the second sliding plate 4431 is slidably connected to the front end of the first sliding plate 441 and is used to buffer the reagent bottle and operating tool when they fall; the two telescopic plates 4432 are slidably sleeved on the front end of the second sliding plate 4431 and have an inclined surface at the top; the two moving rods 4433 are slidably connected to the bottom of the second sliding plate 4431 and correspond to the inclined surface, so that the operating tool is released when the telescopic plates 4432 move backward.

[0048] Multiple telescopic rods 4434 are axially mounted inside the first sliding plate 441. Springs 4435 are sleeved around each telescopic rod 4434 and are compressed when the telescopic rods 4434 move outwards. The rear end of the second sliding plate 4431 is connected to the telescopic rods 4434, allowing it to move back and forth. Simultaneously, both sides of the second sliding plate 4431 are slidably connected to both sides of the sealed channel 41. The rear ends of two telescopic plates 4432 are slidably connected to the front end of the second sliding plate 4431 via springs 4436. A track is provided on the bottom front end of the second sliding plate 4431 to accommodate the telescopic plates 4432. The rear end of spring 4436 is connected within the track. When the telescopic plates 4432 retract into the second sliding plate 4431, spring 4436 is compressed. The two telescopic plates 4432 are symmetrically arranged, and the directions of the two inclined surfaces are also symmetrical. The front top of the sliding plate 4431 has two guide grooves that match the moving rods 4433. The bottom ends of the two moving rods 4433 are slidably connected to the guide grooves via guide blocks. The moving rods 4433 are perpendicular to the telescopic plate 4432 and their bottoms abut against the inclined surface. When the two telescopic plates 4432 retract, the two moving rods 4433 will move away from each other. Specifically, since the telescopic plate 4432 is located in front of the moving rods 4433, the telescopic plate 4432 contacts the sealing door 43 first. The force of the spring 4436 is less than the force of the torsion spring on the sealing door 43, so the telescopic plate 4432 is compressed and retracted by the sealing door 43. When the sliding plate 441 continues to slide down, the telescopic plate 4432 is retracted into the sliding plate 4431. The elastic force of the spring 4436 acts on the sealing door 43. The pushing force of the sliding plate 441 and the sliding plate 4431 also acts on the sealing door 43, causing the sealing door 43 to be squeezed open. Then, the reagent bottle and the operating tool can fall into the placement slot 131 due to their own weight.

[0049] The working principle of this embodiment is as follows: When reagent bottles and operating tools successively enter the sealed channel 41 and fall onto slide plate 1 441 and slide plate 2 4431, the two moving rods 4433 move closer together to block the reagent bottles and operating tools. As more tools fall onto slide plate 2 4431, the downward sliding force on slide plate 1 441 and slide plate 2 4431 gradually increases. Since there are still tools blocking the sealing door 1 42 at the sealing door, ropes 1 444 and 2 445 can temporarily control slide plate 1 441 at the rear end of the sealed channel 41. At this time, slide plate 2 4431 will slide forward due to pressure. Increase the tension of slide plate 441. When all the tools enter the sealed channel 41, slide plate 441 will slide forward due to pressure. At the same time, rope 444 will pull the sealed door 42 upward to close the sealed door 42 and push the gravity component 46 back into the top slot of the track 45. When slide plate 441 slides forward, rope 445 also slides, causing gravity component 442 to move backward. When slide plate 441 and slide plate 4431 slide forward, causing the telescopic plate 4432 to be squeezed backward by the sealed door 43, the inclined plane pushes the two moving rods 4433 away from each other, and then the reagent bottle and operating tools slide out of the sealed channel 41 and fall into the operating area 13. After there is nothing on the first skateboard 441 and the second skateboard 4431, the second skateboard 4431 is pulled backward by the telescopic rod 4434. When the telescopic plate 4432 slides backward, the sealing door 2 43 automatically closes. The gravity component 2 442 moves forward and pulls the first skateboard 441 backward through the rope 2 445, so that the sliding component 44 returns to its original position.

[0050] Specific application examples In medical testing and clinical microbiology laboratories, this contamination-proof bacterial culture device is used for the isolation, culture, and drug sensitivity testing of pathogenic bacteria (such as Staphylococcus aureus, Escherichia coli, and Mycobacterium tuberculosis) in clinical samples such as blood, urine, and sputum. It can avoid cross-contamination between different patient samples and prevent highly pathogenic pathogens from being exposed to operators.

[0051] The specific operating procedure for this device is as follows: Close the flip cover 15 to ensure the chamber 1 is completely sealed; turn on all the sterilization lamps 6 inside the chamber 1 (simultaneously irradiate the tool sterilization chamber 11, operating area 13, and culture chamber for 15–30 minutes); place the reagent bottles (containing culture medium) and operating tools (inoculation loops, forceps, pipette tips) to be sterilized onto the rolling rod 31 in the tool sterilization chamber 11; turn on the rolling rod 31 and rotate it slowly to ensure that all surfaces are evenly exposed to ultraviolet light for sterilization; adjust the height of the telescopic component 32 to tilt the rolling rod 31, causing the reagent bottle to roll forward and press down on the sealing door 42, allowing it to slide open along the track 45; the reagent bottle falls onto the sliding plate 4431 of the buffer component 443, generating recoil; when the sliding plate 441 reaches the front end of the sealing channel 41, the two telescopic plates 4432... The pressure pushes the sealing door 2 43 to rotate and open, and its top inclined surface drives the moving rod 4433 to separate outward; the moving rod 4433 opens, releasing the clamping state, and the reagent bottle falls smoothly into the operating area 13; the sliding plate 1 441 then resets, the sealing door 2 43 closes automatically, and the passage is resealed; the experimenter puts their hands into rubber gloves; the sealing door 3 22 of the bacterial culture chamber 12 is opened, the auxiliary push rod 23 is activated, the designated culture bottle is pushed out to the clamp 5 position and clamped; the sterilized inoculation loop is used to pick up the sample and connect it to the culture bottle; after inoculation, the sealing door 3 22 is closed, and the bacterial culture chamber 12 is restored to airtightness; after the experiment, the flip cover 15 is opened and all consumables are taken out; the whole chamber 1 ultraviolet disinfection program is restarted again (30 minutes).

[0052] In summary, although embodiments of the present invention have been shown and described, those skilled in the art will understand that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the claims and their equivalents.

Claims

1. A contamination-resistant bacterial culture device, comprising a housing (1), a transparent flip-top (15) on the housing (1), and a tool disinfection chamber (11) and a bacterial culture chamber (12) disposed inside the housing (1); characterized in that, Also includes: The operating area (13) is located inside the box (1) and between the tool disinfection room (11) and the bacterial culture room (12); The culture bottle control mechanism (2) is located inside the bacterial culture chamber (12) and is used to adjust the position of the culture bottle; The disinfection unit (3) is set up in the tool disinfection room (11) for disinfecting reagent bottles and operating tools. The isolation facility (4) is set between the tool disinfection room (11) and the operation area (13) to actively transfer sterilized reagent bottles and operation tools to the operation area (13).

2. The anti-contamination bacterial culture device according to claim 1, characterized in that, The operating area (13) is provided with a clamp (5) for holding culture bottles; the side wall of the operating area (13) is provided with a mounting hole (14) for installing rubber gloves.

3. The anti-contamination bacterial culture device according to claim 1, characterized in that, The isolation facility (4) includes: The sealed channel (41) is inclined and connected to the tool disinfection chamber (11) at the rear end and to the operating area (13) at the front end. The sealing door (42) is slidably connected to the rear end of the sealing channel (41) and opens automatically when it comes into contact with the reagent bottle and operating tools; Sealing door 2 (43) is rotatably connected to the front end of sealing channel (41); The sliding component (44) is slidably disposed inside the sealed channel (41) for carrying the operating tool and actively closing the sealed door (42).

4. The anti-contamination bacterial culture device according to claim 3, characterized in that, A track (45) is provided between the sealing door (42) and the disinfection mechanism (3); a slider (421) matching the track (45) is provided at the rear end of the sealing door (42); a gravity component (46) matching the track (45) is movably provided at the top of the track (45), and the gravity component (46) is used to press down the slider (421) to move the sealing door (42).

5. The anti-contamination bacterial culture device according to claim 4, characterized in that, The sliding component (44) includes: The back end of the slide plate (441) is connected to the bottom of the sealing door (42) by rope (444), and the front and rear sides are connected to the inner wall of the sealing channel (41) by rope (445). Gravity component two (442) is connected to the end of rope two (445) away from the first slide plate (441) and is used to pull the first slide plate (441) upward; A buffer (443) is slidably connected to the front end of a slide plate (441) to protect the reagent bottle during transfer.

6. The anti-contamination bacterial culture device according to claim 5, characterized in that, The buffer (443) includes: Slide 2 (4431) is slidably connected to the front end of slide 1 (441) and is used to cushion the reagent bottle and operating tool when they fall. Two telescopic plates (4432) are slidably fitted onto the front end of the second sliding plate (4431) and have a slope on the top; Two movable rods (4433) are slidably connected to the bottom of the slide plate (4431) and correspond to the inclined plane, and are used to release the operating tool when the telescopic plate (4432) moves backward.

7. The anti-contamination bacterial culture device according to claim 1, characterized in that, The disinfection mechanism (3) includes: Multiple rollers (31) are arranged side by side in the tool sterilization chamber (11) for transporting reagent bottles and operating tools when rotating; Telescopic component (32) is located below the rolling rod (31) and is used to adjust the height of the rolling rod (31); A separator (33) is located to the left of the rolling bar (31) to separate the reagent bottle and the operating tool.

8. The anti-contamination bacterial culture device according to claim 7, characterized in that, The spacer assembly (33) includes: A fixing plate (331) is set inside the tool disinfection chamber (11) and located to the left of the rolling rod (31), and has an opening (121). A partition (332) is slidably installed in the tool sterilization chamber (11) to separate reagent bottles and operating tools; The displacement plate (333) is slidably mounted on the fixed plate (331) for opening and closing the opening (121). The pusher (334) is connected to the partition (332) at one end and the displacement plate (333) at the other end, and is used to push the partition (332) and the displacement plate (333) simultaneously.

9. The anti-contamination bacterial culture device according to claim 2, characterized in that, The culture flask control mechanism (2) includes: The mounting plate (21) is rotatably connected to the bacterial culture chamber (12) and has multiple mounting slots (211) for placing culture bottles. Sealed door three (22) is slidably connected to the rear side of bacterial culture chamber (12); The auxiliary push rod (23) is set on the mounting plate (21), with its end corresponding to the mounting groove (211), and is used to push the culture bottle onto the clamp (5).

10. The anti-contamination bacterial culture device according to claim 1, characterized in that, The box (1) is equipped with multiple disinfection lamps (6).

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