Schizophyllum commune as well as product and application thereof

Abstract

The invention discloses schizophyllum commune as well as a product and application thereof, the schizophyllum commune is schizophyllum commune SY-2022-F11, and the preservation number of the schizophyllum commune SY-2022-F11 in the China General Microbiological Culture Collection Center (CGMCC) is CGMCC No. 42266. The schizophyllum commune is a schizophyllum commune strain. The schizophyllum commune is added into cosmetics in the form of schizophyllum commune fermentation liquor in the skin whitening process. The fermentation liquor of the schizophyllum commune SY-2022-F11 disclosed by the invention has relatively high tyrosinase inhibition rate and antioxidant activity.

Description

Technical Field

[0001] This invention belongs to the field of biological skin whitening technology, and in particular relates to a type of Schizophyllum commune, its products, and applications. Background Technology

[0002] Schizophyllum commune is a well-known edible white-rot fungus that produces a variety of secondary metabolites. First recorded in the *Xinhua Materia Medica*, it is a rare fungus used for both food and medicine, possessing the effects of clearing the liver and improving eyesight, and nourishing the body. According to literature such as *Medicinal Fungi*, this fungus is "neutral in nature, sweet in taste, bitter in flavor, slightly cold, and non-toxic. It has significant effects on treating night sweats in children, gynecological diseases, neurasthenia, dizziness, and tinnitus." Schizophyllum commune contains all eight essential amino acids and is rich in various trace elements such as zinc, iron, calcium, phosphorus, selenium, and germanium, giving it high medicinal value. Schizophyllum commune belongs to the genus *Schizophyllum* of the family Schizophyllaceae and is a rare medicinal and edible fungus. Its hyphae are white, branched, and curved, with a diameter of 1–2 μm. It grows radially from the inoculation point, where the hyphae are yellow. After about 8 days of development, the hyphae twist and form fruiting body primordia.

[0003] In existing technologies, research on Schizophyllum commune fermentation broth mainly focuses on polysaccharide extraction and immunomodulatory applications. Through a liquid deep fermentation process using Schizophyllum commune, under conditions of 3.0% glucose, 0.5% soybean flour, 0.2% yeast extract, 0.1% KH₂PO₄, and 0.05% MgSO₄·7H₂O (pH 5.5), at 26–28℃ and 100–110 r / min, a polysaccharide yield of 5.0 g / L was achieved. The unique triple helix structure of its polysaccharides can adsorb water molecules, promote the production of natural moisturizing factors, maintain the moisture content of the stratum corneum, and enhance skin hydration. For example, Chinese patent application CN115141863A uses a whole plant culture medium (carrot, strawberry, oat) to culture Schizophyllum commune and increases the polysaccharide content to 3-15 mg / mL; patent CN118126836A screened and obtained Schizophyllum commune Wbsh-01 with accession number CGMCCNo.40388, and increased the polysaccharide yield through a secondary fermentation process, but its core application is still anti-inflammatory and antioxidant.

[0004] Currently, there is relatively little research on the skin-whitening effects of Schizophyllum commune fermentation broth. Chinese patent application CN115141863A discloses that Schizophyllum commune can inhibit tyrosinase activity (in vitro inhibition rate of 30% to 80%) and melanin synthesis, but this only indirectly enhances the skin-whitening activity through "optimization of whole plant culture medium," and cannot prove that the efficacy originates from strain specificity rather than culture medium components. Summary of the Invention

[0005] In order to overcome the problems in the prior art, the present invention provides a Schizophyllum commune and its products and applications. A strain of Schizophyllum commune with whitening effect was screened and its fermentation broth has a high tyrosinase inhibition rate and antioxidant activity.

[0006] To solve the above-mentioned technical problems, the technical solution proposed by this invention is as follows: This invention provides a Schizophyllum commune, specifically Schizophyllum commune SY-2022-F11, which has the accession number CGMCC No.42266 at the China General Microbiological Culture Collection Center.

[0007] As an optional implementation, the nucleotide sequence of Schizophyllum commune provided by the present invention is shown in SEQ ID NO.1.

[0008] Based on the same technical concept, the present invention also mentions the application of the above-mentioned Schizophyllum commune in skin whitening.

[0009] As an optional implementation, in the application provided by the present invention, the Schizophyllum commune is added to cosmetics in the form of Schizophyllum commune fermentation liquid during the skin whitening process.

[0010] As an optional implementation, in the application provided by the present invention, the Schizophyllum commune fermentation broth achieves whitening effects by inhibiting tyrosinase.

[0011] As an optional implementation, in the application provided by the present invention, the inhibition rate of the Schizophyllum commune fermentation broth against tyrosinase is 85-99%.

[0012] Based on the same technical concept, the present invention also mentions the application of the above-mentioned Schizophyllum commune in skin anti-oxidation.

[0013] As an optional implementation, in the application provided by the present invention, the antioxidant effect of *Schizophyllum commune* is achieved through *Schizophyllum commune* fermentation broth, wherein the antioxidant activity of the *Schizophyllum commune* fermentation broth exhibits at least one of the following: (1) DPPH scavenging rate; (2) ABTS inhibition rate; (3) Total antioxidant capacity (T-AOC).

[0014] As an optional implementation, in the application provided by the present invention, the culture medium used to prepare the Schizophyllum commune fermentation broth includes 10-30 g / L of carbon source, 10-30 g / L of malt extract powder, and 1-3 g / L of nitrogen source; the nitrogen source is a mixture of peptone and yeast powder.

[0015] As an optional implementation, in the application provided by the present invention, the culture medium used to prepare the Schizophyllum commune fermentation broth includes 20 g / L of carbon source, 20 g / L of malt extract powder, and 2 g / L of nitrogen source.

[0016] As an optional implementation, in the application provided by the present invention, the method for preparing the *Schizophyllum commune* fermentation broth includes the following steps: S1. Prepare *Schizophyllum commune* SY-2022-F11 bacterial culture; S2. Prepare the fermentation medium, sterilize it, and set it aside for later use; S3. Add the Schizophyllum commune SY-2022-F11 bacterial solution from S1 to the fermentation medium in S2 for fermentation. The fermentation temperature is 28-32℃ and the fermentation speed is 160-200 rpm. After fermentation, filter to obtain the fermentation broth.

[0017] Based on the same technical concept, the present invention also provides a bacterial agent with whitening effect, wherein the bacterial agent contains the fermentation broth of the above-mentioned Schizophyllum commune.

[0018] Compared with the prior art, the beneficial effects of the present invention are as follows: A strain of *Schizophyllum commune* with a high tyrosinase inhibition rate was obtained through screening. Optimal fermentation conditions were determined by comparing different culture media. The resulting *Schizophyllum commune* SY-2022-F11 fermentation broth exhibited a tyrosinase inhibition rate as high as 96.43%, significantly higher than the 11.52% of ordinary fermentation broth. Furthermore, the *Schizophyllum commune* SY-2022-F11 fermentation broth obtained in this invention also showed a tyrosinase inhibition rate of approximately 90% at a concentration of 0.5%, far superior to that of ordinary *Schizophyllum commune* fermentation broth; its antioxidant capacity was also superior to that of ordinary *Schizophyllum commune*. Detailed Implementation

[0019] To facilitate understanding of the present invention, the present invention will be described more fully and in detail below in conjunction with the specification and preferred embodiments, but the scope of protection of the present invention is not limited to the following specific embodiments.

[0020] Unless otherwise defined, all technical terms used herein have the same meaning as commonly understood by those skilled in the art. The technical terms used herein are for the purpose of describing particular embodiments only and are not intended to limit the scope of the invention.

[0021] Unless otherwise specified, all raw materials, reagents, instruments and equipment used in this invention can be purchased from the market or prepared by existing methods.

[0022] Example 1 This case study demonstrates the isolation and identification of Schizophyllum commune. Isolation of *Schizophyllum commune*: In a clean bench, *Cordyceps sinensis* was washed three times with sterile water and the surface moisture was absorbed with sterilized gauze. It was then crushed, and the crushed liquid was diluted (10-1). -1 10 -2 10 -3 10 -4 10 -5 10 -6 Spread the culture onto Bengal red agar or PDA medium supplemented with chloramphenicol and incubate at 28°C until mycelia grow in the medium. After incubation, isolate and purify the colonies based on their morphology.

[0023] Molecular biological identification: DNA was extracted from the target strain using a fungal genomic DNA extraction kit. The extracted DNA was sent to Sangon Biotech (Shanghai) Co., Ltd. for sequencing. The strain was identified as *Schizophyllum commune*, strain number SY-2022-F11. The nucleotide sequence of *Schizophyllum commune* is shown in SEQ ID NO.1.

[0024] Strain deposited at the China General Microbiological Culture Collection Center (CGMCC), address: No. 3, No. 1 Beichen West Road, Chaoyang District, Beijing 100101, China. The deposit date is September 19, 2025, the accession number is CGMCCNo. 42266, and the classification name is Schizophyllum commune.

[0025] Example 2 This implementation example describes the cultivation of Schizophyllum commune SY-2022-F11. The culture medium includes the following ingredients: 20 g / L glucose, 20 g / L malt extract, 1 g / L yeast extract, 1 g / L peptone, and natural pH.

[0026] The SY-2022-F11 fermentation broth process includes the following steps: 1) Culture medium preparation: Prepare the culture medium according to the above formula and sterilize it at high temperature for later use.

[0027] 2) Fermentation culture: Using a sterilized pipette tip, take the bacterial suspension of Schizophyllum commune SY-2022-F11 and inoculate it into a sterilized culture medium at an inoculation amount of 5%. Culture at 30℃ and 180rpm for 3 days to obtain SY-2022-F11 fermentation broth.

[0028] The following are comparative examples of the settings in this embodiment: Comparative Example 1: The culture medium used for the fermentation broth of *Schizophyllum commune* SY-2022-F11 included the following ingredients: 10 g / L yeast extract, 20 g / L peptone, 20 g / L glucose, and natural pH. All other ingredients and preparation processes were the same as those used in the fermentation broth of Example 2.

[0029] Comparative Example 2: The culture medium used for the fermentation broth of *Schizophyllum commune* SY-2022-F11 included the following raw materials: 6 g / L potato extract powder, 20 g / L glucose, and natural pH. All other materials and preparation processes were the same as those used in the fermentation broth of Example 2.

[0030] The inhibition rate of tyrosinase by the fermentation broths prepared in Example 2, Comparative Example 1, and Comparative Example 2 was determined: 1.1 Reagent Preparation (1) Prepare a PBS buffer solution with pH 6.8 by weighing 6.8 g of potassium dihydrogen phosphate and 0.94 g of sodium hydroxide, dissolving them in distilled water and making up to 1000 mL in a volumetric flask.

[0031] (2) L-tyrosine solution (prepare fresh): accurately weigh 0.0272 g of L-tyrosine, add it to PBS solution at pH 6.8 to dissolve and make up to 100 mL in a volumetric flask.

[0032] (3) 200U enzyme activity tyrosinase solution (prepare and use immediately): 25KU Solarbio tyrosinase is dissolved in 12.5mL of pH6.8 PBS solution to obtain 2KU tyrosinase solution. Store at -20℃. When using, take an appropriate amount of 2KU tyrosinase solution and dilute it 10 times with pH6.8 PBS solution.

[0033] 1.2 Experimental Procedure Prepare solutions A, B, and C according to Table 1 using a pipette, add them to a 96-well plate, and finally add solution D. Mix thoroughly and incubate at 37°C for 10 min. Measure the absorbance A of the reaction solution at 475 nm and calculate its inhibition rate against tyrosinase.

[0034] Table 1: Composition of the reaction solution

[0035] 1.3 Calculation formula: Inhibition rate = [1 - (sample A - blank A) / (A0 - A1)] × 100%.

[0036] 1.4 Experimental Results The comparison results of tyrosinase inhibition rate of Schizophyllum commune SY-2022-F11 fermentation broth obtained from different culture media in Example 2 and Comparative Examples 1-2 are shown in Table 2. 100% represents the original solution, 2% represents a 50-fold dilution, and so on.

[0037] Table 2: Tyrosinase inhibition rate of *Schizophyllum commune* SY-2022-F11 fermentation broth in Example 2 and Comparative Examples 1-2

[0038] As shown in Table 2, the tyrosinase inhibition rate of the fermentation broth prepared by the culture medium in Example 2 is significantly better than that of Comparative Example 1 and Comparative Example 2. Therefore, the fermentation culture medium formula of Schizophyllum commune strain SY-2022-F11 used in this invention is: glucose 20g / L, malt extract powder 20g / L, yeast powder 1g / L, peptone 1g / L, natural pH.

[0039] Example 3 (1) Preparation of fermentation broth of Schizophyllum commune SY-2022-F11 in this invention Preparation of fermentation broth for a strain of Schizophyllum commune SY-2022-F11, wherein the culture medium comprises the following raw materials: 20 g / L glucose, 20 g / L malt extract, 1 g / L yeast extract, 1 g / L peptone, and natural pH.

[0040] The SY-2022-F11 fermentation broth process includes the following steps: 1) Culture medium preparation: Prepare the culture medium according to the above formula and sterilize it at high temperature for later use.

[0041] 2) Fermentation culture: Using a sterilized pipette tip, the bacterial suspension of *Schizophyllum commune* SY-2022-F11 was inoculated into sterilized culture medium and cultured at 30℃ and 180 rpm for 1, 2, 3, and 4 days, respectively, to obtain the *Schizophyllum commune* SY-2022-F11 fermentation broth. After fermentation was completed, the supernatant was collected by centrifugation at 20℃ and 8500 rpm. Fermentation broths for Examples 3-7 were obtained respectively.

[0042] (2) Preparation of fermentation broth of Schizophyllum commune The strains inoculated in the comparative examples were commercially available *Schizophyllum commune*, and cultured for 1, 2, 3, and 4 days, respectively, with other conditions the same as in Example 3. Fermentation broths of Comparative Examples 3 to 7 were obtained.

[0043] Antioxidant performance testing (I) Determination of DPPH inhibition rate of fermentation broth of Schizophyllum commune SY-2022-F11 and common Schizophyllum commune in this invention 1.1 Solution preparation: Prepare a 0.1 mM DPPH solution: Prepare twice, each time dissolving 0.002 g of DPPH in 50 mL of ethanol and storing in the dark; Prepare a sample solution of a specific concentration, with at least 2 mL of stock solution. (The absorbance should be approximately 0.7 when measured using an ELISA reader.) 1.2 Test Procedure: The sample was incubated in the dark at room temperature for 30 minutes, and the absorbance was measured. Three groups of 96-well plates were prepared, with three replicates per group. The amount of sample added per well and the distribution in the 96-well plates are as follows: Sample group: 100 μL sample solution + 100 μL DPPH alcohol solution (3 wells per concentration) Blank group: 100 μL sample solution + 100 μL anhydrous ethanol (3 wells per concentration) Control group: 100 μL DPPH alcohol solution + 100 μL water Measure the absorbance at 517 nm, take the average value, and calculate the DPPH scavenging rate of each concentration of sample using the following formula.

[0044] Clearance rate = (1 - (Sample A - Blank A) / Control A) x 100% The test results are shown in Table 3. All DPPH inhibition rates were determined after the prepared fermentation broth was diluted 5 times.

[0045] Table 3: Comparison of DPPH removal rates in fermentation broths of Examples 3-7 and Comparative Examples 3-7

[0046] As shown in Table 3, after 3 days of fermentation, the DPPH removal effect of the fermentation broth of Schizophyllum commune SY-2022-F11 prepared in this application example is better than that of the fermentation broth of Schizophyllum commune prepared in the comparative example. The fermentation time has a certain influence on this.

[0047] (II) Determination of the inhibition rate of ABTS by the fermentation broth of Schizophyllum commune SY-2022-F11 in this invention 1.1 Solution preparation: (1) PBS buffer: 8g sodium chloride, 0.2g potassium chloride, 0.24g potassium dihydrogen phosphate, and 3.63g disodium hydrogen phosphate dodecahydrate, diluted to 1000ml with distilled water. Adjust the pH to 7.4 with hydrochloric acid or sodium hydroxide.

[0048] (2) ABTS stock solution (7 mmol / L): Take 0.0384 g of ABTS and dilute it to 10 ml in a volumetric flask with distilled water.

[0049] (3) K2S2O8 stock solution (2.45mmol / L): Take 0.0066g of K2S2O8 and dilute it to 10ml in a volumetric flask with distilled water.

[0050] (3) ABTS working solution: Mix ABTS stock solution and K2S2O8 stock solution at a ratio of 1:1, let stand for 12 to 16 hours, and prepare ABTS working solution.

[0051] 1.2 Test Procedure: Take 0.4 mL of ABTS working solution and dilute it with PBS solution. The absorbance at 734 nm at room temperature should be 0.7 ± 0.02. Mix 0.2 mL of ABTS+ working solution with 10 μL of test sample of different concentrations, let it stand at room temperature in the dark for 6 min, and measure the absorbance at 734 nm wavelength, repeating the measurement in triplicate.

[0052] 1.3 Data Processing: The free radical scavenging capacity of ABTS is calculated by the following formula: Clearance rate = (1 - (A sample - A blank) / A control) x 100%.

[0053] In the formula: sample group (sample): sample solution + ABTS working solution; Blank group: Sample solution + PBS buffer; Control group: ABTS working solution + distilled water; The test results are shown in Table 4. All ABTS inhibition rates were determined after the prepared fermentation broth was diluted 5 times.

[0054] Table 4: Comparison of ABTS removal rates in fermentation broths of Examples 3-7 and Comparative Examples 3-7

[0055] The results in Table 4 show that, after 3 days of fermentation, the ABTS removal effect of the fermentation broth of Schizophyllum commune SY-2022-F11 prepared in this application example is better than that of the fermentation broth of Schizophyllum commune prepared in the comparative example.

[0056] (III) Determination of T-AOC in the fermentation broth of *Schizophyllum commune* SY-2022-F11 in this invention 1.1 Reagent Preparation Preparation of ferrous sulfate standard solution: Add AK351-standard from the Total Antioxidant Capacity (T-AOC) test kit to 900 μL of distilled water, and then add 20 μL of concentrated sulfuric acid to prepare a 40 μmol / mL ferrous sulfate standard solution.

[0057] 1.2 Test Procedure According to the instructions of the Total Antioxidant Capacity Test Kit (brand: Bio-Sen), a standard curve was prepared and the samples were measured. The total antioxidant capacity of the samples was calculated using the following formula.

[0058] To prepare the standard curve, a 40 μmol / mL ferrous sulfate standard solution was diluted with distilled water to concentrations of 0.2, 0.1, 0.05, 0.025, 0.0125, 0.00625, 0.003125, and 0.00156 μmol / mL. 100 μL of the standard solution (with distilled water as a blank) was added to 100 μL of reagent AK351-B, and the mixture was thoroughly mixed. After reacting for 10 min, the absorbance at 593 nm was measured. ΔA = Astandard - ABlank was calculated, with the final ferrous ion concentration as the x-axis and ΔA as the y-axis to plot the standard curve, thus obtaining the regression equation.

[0059] Mixture (prepare fresh): Mix reagent A, reagent B and reagent C in a ratio of 10:1:1 and preheat to 37°C before use.

[0060] Add the reagents from Table 5 to the 96-well plate in order, mix thoroughly, react for 10 min, measure the absorbance at 593 nm, calculate ΔA, substitute into the regression equation, and find x.

[0061] Total antioxidant capacity = x * Vreverse total / Vsample = 34 * x Table 5: Amount of reagents added

[0062] The test results are shown in Table 6.

[0063] Table 6: Comparison of T-AOC in fermentation broths of Examples 3-7 and Comparative Examples 3-7

[0064] As shown in Table 6, after 3 days of fermentation, the total antioxidant capacity of the Schizophyllum commune SY-2022-F11 fermentation broth prepared in this application example is better than that of the Schizophyllum commune fermentation broth prepared in the comparative example, indicating that the fermentation time has a certain influence.

[0065] (iv) Determination of polysaccharide content in the fermentation broth of Schizophyllum commune SY-2022-F11 in this invention 1.1 Solution preparation: 1.1.1 Preparation of glucose standard solution: Accurately weigh 100 mg of analytical grade glucose that has been dried to constant weight in an oven at 105℃, dissolve it in an appropriate amount of distilled water, and then dilute to 100 mL in a volumetric flask. Store in a refrigerator for later use.

[0066] 1.1.2 Preparation of 5% phenol solution: After dissolving phenol by heating, take 5 g of phenol solution in pure water and dilute to 100 mL in a volumetric flask for later use.

[0067] 1.2. Test Procedure 1.2.1 Glucose Standard Curve Determination Construction of glucose standard curve: Prepare glucose solutions of 10, 20, 30, 40, 60, and 80 mg / mL. Take 100 μL of glucose solution into a centrifuge tube, add 100 μL of phenol (5%) and 500 μL of concentrated sulfuric acid, mix well, let stand for 30 min, and measure the absorbance value of the reaction solution at 490 nm. Plot the glucose standard curve with glucose content as the abscissa and absorbance at 490 nm as the ordinate.

[0068] 1.2.2 Extraction of polysaccharides from fermentation broth The fungal fermentation broth was centrifuged (12000 rpm, 5 minutes), and 0.25 mL of the supernatant was taken. 0.75 mL of ethanol was added, the mixture was shaken and mixed, and the mixture was allowed to stand in a refrigerator at 4°C for 24 hours. The mixture was centrifuged again (12000 rpm, 5 minutes) and then reconstituted with 1 mL of distilled water and diluted to an appropriate ratio for later use.

[0069] 1.2.3 Determination of polysaccharide content Determination of fungal polysaccharide content: Take 100 μL of the test solution into a 2 mL centrifuge tube, add 100 μL of phenol (5%) and 500 μL of concentrated sulfuric acid, let stand for 30 min, and then measure the absorbance of the reaction solution at 490 nm. Calculate the fungal polysaccharide content of the fermentation broth according to the standard curve and dilution factor. The test results are shown in Table 7.

[0070] Table 7: Comparison of polysaccharide content in fermentation broths of Examples 3-7 and Comparative Examples 3-7

[0071] As can be seen from Table 7, after 3 days of fermentation, the polysaccharide content of the fermentation broth of Schizophyllum commune SY-2022-F11 prepared in this application example is lower than that of the fermentation broth of Schizophyllum commune prepared in the comparative example, indicating that there is indeed a difference between the Schizophyllum commune obtained by this invention and ordinary Schizophyllum commune.

[0072] (V) Determination of tyrosinase inhibition rate of fermentation broth of Schizophyllum commune SY-2022-F11 in this invention The tyrosinase inhibition rates of the original fermentation broths prepared in Examples 3-7 and Comparative Examples 3-7 of this invention are shown in Table 8. The tyrosinase inhibition rates after diluting the fermentation broth 200 times are shown in Table 9. Table 8: Results of tyrosinase inhibition rate of the original fermentation broth in Examples 3-7 and Comparative Examples 3-7

[0073] The results in Table 8 show that, throughout the fermentation process, the tyrosinase inhibition rate of the SY-2022-F11 fermentation broth prepared in this application was consistently significantly higher than that of the Schizophyllum commune fermentation broth prepared in the comparative example.

[0074] Table 9: Results of tyrosinase inhibition rate of fermentation broth dilutions in Examples 3-7 and Comparative Examples 3-7

[0075] The results in Table 9 show that on the 3rd-4th day of fermentation, the fermentation broth of Schizophyllum commune SY-2022-F11 prepared in this application, after being diluted 200 times, still showed a tyrosinase inhibition rate close to 90%, indicating that the fermentation broth of Schizophyllum commune SY-2022-F11 obtained in this invention has excellent efficacy.

[0076] Based on the comprehensive evaluation of its whitening and antioxidant effects, the optimal fermentation time for the *Schizophyllum commune* SY-2022-F11 strain of the present invention is 3 days.

[0077] The above description, in conjunction with specific preferred embodiments, provides a further detailed explanation of the present invention. However, it should not be construed that the specific implementation of the present invention is limited to these descriptions. For those skilled in the art, various simple deductions or substitutions can be made without departing from the concept of the present invention, and all such modifications and substitutions should be considered within the scope of protection of the present invention.

Claims

1. A Schizophyllum species, characterized in that, The Schizophyllum is Schizophyllum commune SY-2022-F11, which has a preservation number of CGMCC No.42266 in China General Microbiological Culture Collection Center.

2. The Schizophyllum for use in skin whitening according to claim 1.

3. Use according to claim 2, characterized in that, The Schizophyllum is added to cosmetics in the form of Schizophyllum fermentation broth in the process of skin whitening.

4. Use according to claim 3, characterized in that, The Schizophyllum fermentation broth achieves whitening effect by inhibiting tyrosinase.

5. Use according to claim 3, characterized in that, The inhibition rate of the Schizophyllum fermentation broth on tyrosinase is 85-99%.

6. The Schizophyllum for use in skin antioxidation according to claim 1.

7. Use according to claim 6, characterized in that, The antioxidation effect of the Schizophyllum is achieved by Schizophyllum fermentation broth, and the antioxidant activity of the Schizophyllum fermentation broth is at least one of the following: (1) DPPH clearance rate; (2) ABTS inhibition rate; (3) total antioxidant capacity (T-AOC).

8. Use according to claim 3, characterized in that, The culture medium used for preparing the Schizophyllum fermentation broth comprises 10-30 g / L of carbon source, 10-30 g / L of malt extract powder, and 1-3 g / L of nitrogen source; the nitrogen source is a mixture of peptone and yeast powder.

9. Use according to claim 3, characterized in that, The preparation method of the Schizophyllum fermentation broth comprises the following steps: S1, preparing Schizophyllum SY-2022-F11 bacterial solution; S2, preparing fermentation medium and sterilizing for standby; S3, adding the Schizophyllum SY-2022-F11 bacterial solution in S1 to the fermentation medium in S2 for fermentation, the fermentation temperature is 28-32℃, the rotation speed is 160-200 rpm during fermentation, and the fermentation broth is obtained after filtration. 10.A bacterial agent having a whitening effect, characterized in that, The bacterial agent contains the fermentation broth of the Schizophyllum as described in claim 1.

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