Universal odorless body surface cleaning antibacterial composition for cats and dogs

Abstract

The invention relates to the technical field of pet nursing and cleaning supplies, and discloses a cat and dog universal aroma-free body surface cleaning antibacterial composition, which comprises deionized water, a surfactant, a deodorant, a buffer agent, a biological enzyme system, a bacteriostatic agent, a preservative, a stabilizer and a pH regulator, the composition is a colorless transparent liquid, the pH value is 6.3-6.7, the pH value is 6.7-6.7, and the pH value is 6.7-6.7. The viscosity is adjusted by hydroxyethyl cellulose, the content of silver ions is less than or equal to 0.002%, and the initial value of enzyme activity is more than or equal to 85%. According to the universal odorless body surface cleaning antibacterial composition for cats and dogs, a mild cleaning system formed by compounding nonionic and ampholytic surfactants (hydroxyl glucoside and cocamidopropyl betaine) is adopted, and the pH value (6.3-6.7) is accurately controlled, so that the skin of cats and dogs can be cleaned without irritation, and the antibacterial effect is good. A biological enzyme decomposition system (protease, urease and lipase) and a silver citrate antibacterial system are integrated, and a hydroxypropyl-beta-cyclodextrin molecule embedding deodorization technology is supplemented, so that three effects of cleaning, bacteriostasis and deodorization are integrated.

Description

Technical Field

[0001] This invention relates to the field of pet care and cleaning products technology, specifically to a fragrance-free antibacterial composition for cats and dogs. Background Technology

[0002] Pet care and cleaning requires comprehensive consideration of multiple aspects, including daily cleaning, skin disease prevention, and market trends, to ensure the health and comfort of pets. At the same time, with market development, smart devices and environmentally friendly products are gradually becoming new trends in pet care.

[0003] Most pet surface cleaning products on the market contain fragrances, pigments, alcohol, or irritating chemical antibacterial ingredients. Long-term use may lead to dry skin, allergies, rough fur, and even the risk of licking poisoning in pets. At the same time, most products separate cleaning and antibacterial functions, making it impossible to effectively inhibit the growth of microorganisms while gently cleaning. They also have limited effectiveness in removing pet odors (such as urine stains and body odor). In addition, cats and dogs have differences in skin pH, fur structure, and metabolic products, so existing products often cannot truly be "suitable for both cats and dogs" and may cause discomfort to certain types of pets. Summary of the Invention

[0004] (a) Technical problems to be solved To address the shortcomings of existing technologies, this invention provides a fragrance-free antibacterial body cleaning composition suitable for both cats and dogs. It has the advantages of gentle formula, high cleaning efficiency, long-lasting antibacterial effect, strong deodorization, high stability, and suitability for both cats and dogs. It solves the problems of existing pet cleaning products, such as high irritation, limited functionality, poor species adaptability, insufficient stability, and residue or dryness and tightness after use.

[0005] (II) Technical Solution To achieve the above-mentioned formula's objectives of being gentle, highly effective in cleaning, long-lasting in antibacterial, powerfully deodorizing, highly stable, and suitable for both cats and dogs, this invention provides the following technical solution: A fragrance-free antibacterial body cleaning composition suitable for both cats and dogs, comprising deionized water, surfactant, deodorizer, buffer, biological enzyme system, antibacterial agent, preservative, stabilizer, and pH adjuster. The composition is a colorless and transparent liquid with a pH value of 6.3-6.7, viscosity adjusted by hydroxyethyl cellulose, silver ion content ≤0.002%, and initial enzyme activity ≥85%.

[0006] Furthermore, the surfactant includes hydroxyglucosinolate and cocamidopropyl betaine, both at a content of 1.5% w / w, and is a nonionic and amphoteric surfactant compound system.

[0007] Furthermore, the deodorizing agent is hydroxypropyl-β-cyclodextrin at a content of 2.0% w / w, used for molecular capture and odor neutralization.

[0008] Furthermore, the bio-enzyme system includes protease, urease, and lipase, with a total content of 0.6% w / w, and is equipped with an enzyme stabilizer.

[0009] Furthermore, the antibacterial agent is silver citrate with a content of 0.2% w / w, which translates to a silver ion content of ≤0.002%, and it needs to be stored away from light.

[0010] Furthermore, after being stored at 40°C for 30 days and then frozen at -5°C for 24 hours and thawed, the composition showed no stratification or precipitation, indicating good stability.

[0011] Furthermore, in skin safety tests, the composition showed no erythema, hair loss, or irritation after being applied to the inner forearm of cats, dogs, and humans for 24-72 hours.

[0012] Furthermore, the production process of the composition includes main liquid preparation, deodorization and buffer system establishment, biological enzyme system activation, antibacterial and preservative system addition, structure and stability control, final adjustment and filling, quality control and sample retention and storage conditions. The specific operation steps are as follows: Preparation of main solution: Add 70.8% of the formula amount of deionized water to a mixing tank, control the temperature at 25℃, and add 1.5% of hydroxyglucosinolate and 1.5% of cocamidopropyl betaine in sequence at a stirring speed of 300 rpm, and stir for 10 minutes each to obtain a uniform, transparent, mild and clean base solution. Deodorization and buffer system establishment: Slowly add 2.0% hydroxypropyl-β-cyclodextrin to the base solution obtained in step 1 and stir for 30 minutes to completely dissolve it; Separately, dissolve 0.8% sodium bicarbonate in 50mL of deionized water, filter and add it to the main tank, then add 0.2% sodium citrate buffer solution to stabilize the pH of the system between 6.8 and 7.0. Activation of the bio-enzyme system: Cool the system to below 25°C, add 0.6% of the total content of the compound enzyme, and simultaneously add 0.2% of the enzyme stabilizer. Stir for 15 minutes while avoiding the generation of bubbles, and then test the transparency and enzyme activity. Addition of antibacterial and preservative system: Add 0.2% silver citrate solution under light-protected conditions and stir gently for 10 minutes; then add 0.25% phenoxyethanol and 0.15% ethylhexylglycerin in sequence, stir and mix evenly, and check that the pH of the system at this time should be 6.4-6.6; Structure and stability control: 0.3% hydroxyethyl cellulose was slowly and evenly added under stirring at 400 rpm, and stirring was continued for 40 minutes until completely dissolved, and the viscosity of the system was stable; then it was allowed to stand to degas or vacuum degassed (-0.08 MPa), and filtered through a 0.45 μm filter membrane to ensure transparency ≥95%; Final adjustment and filling: The pH of the system is finely adjusted to 6.5±0.2 using citric acid or sodium hydroxide solution. After final filtration through a 0.22μm filter membrane, the system is filled at 22±2℃ and packaged in light-proof PET or HDPE bottles with leak-proof nozzles. Quality control and sample retention: Each batch of product is tested for appearance, pH, viscosity, silver ion content, microbial indicators and enzyme activity; at least 200 mL of each batch is retained and sealed for 12 months for stability traceability. Storage conditions: The finished product should be stored in a dark, dry environment at a temperature ≤25℃. The shelf life is 18 months in the unopened state.

[0013] (III) Beneficial Effects Compared with the prior art, the present invention provides a fragrance-free antibacterial body cleaning composition suitable for both cats and dogs, which has the following beneficial effects: 1. This fragrance-free antibacterial body cleaning composition for cats and dogs uses a gentle cleaning system formulated with nonionic and amphoteric surfactants (hydroxyglucosinolates and cocamidopropyl betaine) and precisely controls the pH value (6.3-6.7) to achieve non-irritating cleaning for cat and dog skin. It solves the problems of dry, itchy, and damaged pet skin caused by existing products containing strong cleaning ingredients or unsuitable pH levels.

[0014] 2. This fragrance-free body cleaning and antibacterial composition for cats and dogs integrates a bio-enzyme decomposition system (protease, urease, lipase) with a silver citrate antibacterial system, and is supplemented with hydroxypropyl-β-cyclodextrin molecular encapsulation deodorization technology. It achieves a three-in-one effect of cleaning, antibacterial and deodorizing, solving the technical problems of traditional products having single function, incomplete removal of body odor (such as urine stains and body odor), and short-lasting microbial inhibition effect.

[0015] 3. This fragrance-free antibacterial body cleaning composition for cats and dogs, through optimized formula stability (such as the addition of enzyme stabilizers and hydroxyethyl cellulose to adjust viscosity) and strict process control (light-protected operation and multi-stage filtration), ensures that the product does not separate or precipitate during long-term storage (tested at -5℃ to 40℃) and shelf life (18 months), and that the enzyme activity remains stable (initial ≥85%). This solves the common industry problems of active ingredients (especially enzymes and silver ions) being easily deactivated, easily discolored, and having unstable systems in similar products. Attached Figure Description

[0016] Figure 1 This is a schematic diagram of the universal fragrance-free body surface cleaning and antibacterial composition for cats and dogs of the present invention; Figure 2 This is a flowchart illustrating the production process of the universal fragrance-free body surface cleaning and antibacterial composition for cats and dogs according to the present invention. Detailed Implementation

[0017] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0018] Please see Figure 1 and Figure 2 A fragrance-free antibacterial body cleaning composition suitable for cats and dogs includes deionized water, surfactant, deodorizer, buffer, biological enzyme system, antibacterial agent, preservative, stabilizer and pH adjuster. The composition is a colorless and transparent liquid with a pH value of 6.3-6.7, viscosity adjusted by hydroxyethyl cellulose, silver ion content ≤0.002%, and initial enzyme activity ≥85%.

[0019] In the case implementation, the surfactants included hydroxyglucosinolate and cocamidopropyl betaine, both at a content of 1.5% w / w, and were a combination system of nonionic and amphoteric surfactants.

[0020] Among them, the compound system, through the synergistic effect of two surfactants with complementary mechanisms, significantly reduces potential skin irritation while ensuring high cleaning power; Specifically, a nonionic surfactant (hydroxyglucosinolate) and an amphoteric surfactant (cocamidopropyl betaine) are compounded in a specific ratio (1:1). This compounding method produces a synergistic effect. Hydroxyglucoside has good mildness, foaming properties, and hard water resistance, and can form a gentle protective film on the skin surface, reducing the irritation of other ingredients. Cocamidopropyl betaine has excellent detergency, thickening, and foam stabilizing abilities, while its amphoteric properties ensure that it remains mild at any pH value. Together, they create a gentle cleansing base that can deeply cleanse sebum and dirt while having extremely low skin and eye irritation, perfectly meeting the cleansing needs of pets and sensitive skin.

[0021] In the case implementation, the deodorizing agent was hydroxypropyl-β-cyclodextrin at a content of 2.0% w / w, used for molecular capture and odor neutralization.

[0022] Its core function is to utilize its unique ring-shaped cavity structure to achieve physical encapsulation and isolation of odor molecules; Specifically, hydroxypropyl-β-cyclodextrin molecules are cone-shaped, hydrophobic inside and hydrophilic outside. This structure allows its hydrophobic cavity to selectively encapsulate and fix typical hydrophobic small molecules (such as ammonia, short-chain fatty acids, thiols, etc.) in pet body odor. Through this "subject-object encapsulation" effect, odor-producing molecules are encapsulated within the cyclodextrin cavity, preventing them from evaporating into the air and being detected by the sense of smell. This achieves the effect of neutralizing and eliminating body odor and excrement odor from the source for a long time, rather than simply masking the odor.

[0023] In the case implementation, the bio-enzyme system included protease, urease, and lipase, with a total content of 0.6% w / w, and was equipped with an enzyme stabilizer.

[0024] This system utilizes the synergistic catalytic degradation of multiple enzymes to specifically decompose the main organic components of dirt on the pet's body surface. Specifically: Proteases: Specifically hydrolyze protein-based stains (such as food residue, saliva, and secretions); Lipase: breaks down sebum secreted by sebaceous glands and dietary lipids; Urease: Catalyzes the decomposition of urea, reducing the production of ammonia odor at its source; By using the three enzymes in the above ratio to form a synergistic effect, large molecules and poorly soluble organic dirt are decomposed into small molecules such as peptides, amino acids, glycerol, and fatty acids that are easily soluble in water. This achieves deep and gentle physical cleaning, reduces the amount of surfactant used, and destroys the basis for odor molecule formation.

[0025] In the case study, the antibacterial agent was silver citrate with a content of 0.2% w / w, which translates to a silver ion content of ≤0.002%. It needs to be stored away from light.

[0026] Among them, the antibacterial effect mainly depends on the disruption of various physiological processes of microorganisms by trace amounts of slow-release silver ions (Ag⁺); Specifically, silver citrate, as a silver ion carrier, can slowly release Ag⁺ in an aqueous system. Silver ions have extremely high biological activity, can strongly bind to thiol groups (-SH) on the cell membranes of bacteria, fungi and other microorganisms, and penetrate the cell wall, interfering with intracellular enzyme activity and destroying DNA replication ability. Through the above-mentioned multiple mechanisms of action, silver ions can achieve broad-spectrum and long-lasting antibacterial effects at extremely low concentrations (≤0.002%), effectively inhibiting the excessive proliferation of common pathogenic bacteria (such as Staphylococcus aureus, Escherichia coli, etc.) and Malassezia on the surface of pet skin. Moreover, due to its multiple sites of action, it is not easy to induce drug resistance in microorganisms.

[0027] In the case implementation, after the composition was stored at a high temperature of 40°C for 30 days and then frozen at -5°C for 24 hours and thawed, there was no stratification or precipitation, indicating good stability.

[0028] Its outstanding stability stems from the structural stabilizer (hydroxyethyl cellulose) and buffer system in the formulation system, as well as the structural control and defoaming filtration steps in the production process, which together ensure the physical and chemical stability of the complex multiphase system under extreme temperatures. Specifically: Structural stability: Hydroxyethyl cellulose, as an aqueous phase thickener and stabilizer, forms a uniform network structure in the system, effectively preventing the sedimentation of solid particles and the precipitation and floating of oil phase components. Chemical and environmental stability: The buffer system composed of sodium bicarbonate and sodium citrate can stabilize the product's pH value within a preset range (6.3-6.7), preventing component hydrolysis, enzyme inactivation, or silver ion aggregation caused by pH drift. Simultaneously, light-protected storage and the addition of enzyme stabilizers ensure the activity of both the silver citrate and the biological enzyme system. Through the above-mentioned multi-level stabilization design, the composition successfully passed the accelerated stability test (40℃, 30 days) and freeze-thaw cycle test (-5℃, 24 hours thaw). After the test, the system remained uniform and transparent, without any visible layering, flocculation or precipitation. Moreover, the changes in key activity indicators (such as enzyme activity, pH value and viscosity) were all within acceptable ranges, proving that the product has reliable shelf stability during storage and transportation.

[0029] In the case implementation, the composition showed no erythema, hair loss or irritation after being applied to the inner forearm of cats, dogs and humans for 24-72 hours in skin safety tests.

[0030] Its high skin safety is rooted in the overall design philosophy of the formula, that is, all raw material selection and proportion are based on the principle of low irritation and high biocompatibility, and the potential irritation of the system as a whole is reduced through compounding technology. Specifically: Gentle ingredients: The main cleansing ingredients are plant-derived hydroxyglucosinolates and mild cocamidopropyl betaine, avoiding the use of strong anionic surfactants (such as SLS) that may damage the skin barrier. Physiological compatibility: The final pH value of the product (6.5±0.2) is precisely adjusted to be close to the slightly acidic range of healthy cat, dog and human skin, minimizing irritation caused by pH incompatibility; Eliminating allergens: The formula does not contain alcohol, artificial flavors, pigments, or other known common irritants or allergens; In accordance with the "Cosmetic Safety Technical Specifications" or relevant pet product safety evaluation guidelines, a closed skin patch test was conducted on the product. The results showed that, under continuous application for up to 72 hours, no irritation or allergic reactions such as erythema, edema, papules, or hair loss were observed in the test skin areas of all tested cats, dogs, and human volunteers. This fully verifies that the composition has excellent safety and tolerability even when in contact with the skin for a long time, meeting the design goals of "suitable for both cats and dogs" and "human-pet friendly".

[0031] In the case implementation, the production process of the composition included main liquid preparation, deodorization and buffer system establishment, bio-enzyme system activation, antibacterial and preservative system addition, structure and stability control, final conditioning and filling, quality control and sample retention and storage conditions. The specific operation steps are as follows: Preparation of main solution: Add 70.8% of the formula amount of deionized water to a mixing tank, control the temperature at 25℃, and add 1.5% of hydroxyglucosinolate and 1.5% of cocamidopropyl betaine in sequence at a stirring speed of 300 rpm, and stir for 10 minutes each to obtain a uniform, transparent, mild and clean base solution. Deodorization and buffer system establishment: Slowly add 2.0% hydroxypropyl-β-cyclodextrin to the base solution obtained in step 1 and stir for 30 minutes to completely dissolve it; Separately, dissolve 0.8% sodium bicarbonate in 50mL of deionized water, filter and add it to the main tank, then add 0.2% sodium citrate buffer solution to stabilize the pH of the system between 6.8 and 7.0. Activation of the bio-enzyme system: Cool the system to below 25°C, add 0.6% of the total content of the compound enzyme, and simultaneously add 0.2% of the enzyme stabilizer. Stir for 15 minutes while avoiding the generation of bubbles, and then test the transparency and enzyme activity. Addition of antibacterial and preservative system: Add 0.2% silver citrate solution under light-protected conditions and stir gently for 10 minutes; then add 0.25% phenoxyethanol and 0.15% ethylhexylglycerin in sequence, stir and mix evenly, and check that the pH of the system at this time should be 6.4-6.6; Structure and stability control: 0.3% hydroxyethyl cellulose was slowly and evenly added under stirring at 400 rpm, and stirring was continued for 40 minutes until completely dissolved, and the viscosity of the system was stable; then it was allowed to stand to degas or vacuum degassed (-0.08 MPa), and filtered through a 0.45 μm filter membrane to ensure transparency ≥95%; Final adjustment and filling: The pH of the system is finely adjusted to 6.5±0.2 using citric acid or sodium hydroxide solution. After final filtration through a 0.22μm filter membrane, the system is filled at 22±2℃ and packaged in light-proof PET or HDPE bottles with leak-proof nozzles.

[0032] Quality control and sample retention: Each batch of product is tested for appearance, pH, viscosity, silver ion content, microbial indicators and enzyme activity; no less than 200 mL of each batch is retained and sealed for 12 months for stability traceability.

[0033] Storage conditions: The finished product should be stored in a dark, dry environment at a temperature ≤25℃. The shelf life is 18 months in the unopened state.

[0034] The entire process design follows a progressive principle of “first establishing a mild environment, then adding sensitive active ingredients, and finally building a stable structure”, aiming to solve the compatibility and stability problems of various components such as mild surfactants, thermosensitive bioenzymes, photosensitizing silver ions and polymer thickeners when compounded. Specifically: Establish a stable environment before adding any active or sensitive ingredients. First, complete the construction of the clean base solution and buffer system to ensure that the system is in a mild chemical environment with stable pH (6.8-7.0) and suitable temperature (≤25℃). Stepwise activation and protection of active components: Under suitable conditions, the enzyme system is sequentially introduced and "activated" (through low temperature and protectant) and the silver ion antibacterial agent is "stabilized" (through light protection and gentle mixing) to retain its initial activity to the maximum extent. Post-construction of physical structure: After all active ingredients are mixed evenly, a structure modifier (hydroxyethyl cellulose) is added last. By controlling the shear force (400 rpm) and time (40 minutes), it is fully hydrated to build a uniform network structure, thereby "locking in" the entire formulation system. Defoaming and filtration are then used to ensure the physical state of the final product (transparency ≥95%, no bubbles). Through the above-mentioned orderly and meticulous process, the nine main components with different properties in the formula were successfully integrated into a homogeneous, stable, transparent and highly active liquid system. The sequence, temperature, stirring conditions and light control of each step provided key manufacturing guarantees for ensuring the high stability (passing high temperature / freeze-thaw test), high activity (enzyme activity ≥85%) and batch-to-batch consistency of the final product, enabling the laboratory formula to be successfully transformed into a stable commercial product that can be mass-produced.

[0035] In summary, this universal fragrance-free antibacterial body cleaning composition for cats and dogs utilizes a gentle cleaning system formulated with nonionic and amphoteric surfactants (hydroxyglucosinolates and cocamidopropyl betaine), and precisely controls the pH value (6.3-6.7), achieving non-irritating cleaning for cat and dog skin. It solves the problems of dry, itchy, and damaged pet skin caused by existing products containing strong cleaning ingredients or unsuitable pH levels. By integrating a bio-enzyme decomposition system (protease, urease, lipase) with a silver citrate antibacterial system, and supplementing it with hydroxypropyl-β-cyclodextrin molecular encapsulation deodorization technology, it achieves a three-in-one effect of cleaning, antibacterial, and deodorization. This overcomes the technical challenges of traditional products having limited functionality, incomplete removal of body odors (such as urine stains and body odor), and short-lasting microbial inhibition effects.

[0036] Furthermore, by optimizing the stability of the formulation (such as adding enzyme stabilizers and adjusting viscosity with hydroxyethyl cellulose) and strictly controlling the process (light-protected operation and multi-stage filtration), the product is ensured to remain free from stratification and precipitation during long-term storage (tested at -5℃ to 40℃) and shelf life (18 months), with stable enzyme activity (initial ≥85%). This solves the common industry problems of active ingredients (especially enzymes and silver ions) being easily deactivated, easily discolored, and having unstable systems in similar products.

[0037] It should be noted that, in this document, relational terms such as "first" and "second" are used merely to distinguish one entity or operation from another, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Furthermore, the terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such a process, method, article, or apparatus. Without further limitations, an element defined by the phrase "comprising one..." does not exclude the presence of other identical elements in the process, method, article, or apparatus that includes said element.

[0038] Although embodiments of the invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A fragrance-free antibacterial body cleaning composition suitable for cats and dogs, comprising deionized water, surfactant, deodorizer, buffer, biological enzyme system, antibacterial agent, preservative, stabilizer, and pH adjuster, characterized in that: The composition is a colorless and transparent liquid with a pH of 6.3-6.7, viscosity adjusted by hydroxyethyl cellulose, silver ion content ≤0.002%, and initial enzyme activity ≥85%.

2. The universal fragrance-free antibacterial body cleaning composition for cats and dogs according to claim 1, characterized in that: The surfactants include hydroxyglucosinolate and cocamidopropyl betaine, both at a content of 1.5% w / w, and are a nonionic and amphoteric surfactant compound system.

3. The universal fragrance-free antibacterial body cleaning composition for cats and dogs according to claim 1, characterized in that: The deodorizing agent is hydroxypropyl-β-cyclodextrin at a content of 2.0% w / w, used for molecular capture and odor neutralization.

4. The universal fragrance-free antibacterial body cleaning composition for cats and dogs according to claim 1, characterized in that: The bio-enzyme system includes protease, urease, and lipase, with a total content of 0.6% w / w, and is equipped with an enzyme stabilizer.

5. The universal fragrance-free antibacterial body cleaning composition for cats and dogs according to claim 1, characterized in that: The antibacterial agent is silver citrate with a content of 0.2% w / w, which translates to a silver ion content of ≤0.002%. It needs to be stored away from light.

6. The universal fragrance-free antibacterial body cleaning composition for cats and dogs according to claim 1, characterized in that: The composition exhibits good stability after being stored at 40°C for 30 days and then frozen at -5°C for 24 hours before thawing, without stratification or precipitation.

7. The universal fragrance-free antibacterial body cleaning composition for cats and dogs according to claim 1, characterized in that: In skin safety tests, the composition showed no erythema, hair loss, or irritation when applied to the inner forearm of cats, dogs, and humans for 24-72 hours.

8. The universal fragrance-free antibacterial body cleaning composition for cats and dogs according to claim 1, characterized in that: The production process of the composition includes main liquid preparation, deodorization and buffer system establishment, biological enzyme system activation, antibacterial and preservative system addition, structure and stability control, final adjustment and filling, quality control, sample retention and storage conditions. The specific operation steps are as follows: Preparation of main solution: Add 70.8% of the formula amount of deionized water to a mixing tank, control the temperature at 25℃, and add 1.5% of hydroxyglucosinolate and 1.5% of cocamidopropyl betaine in sequence at a stirring speed of 300 rpm, and stir for 10 minutes each to obtain a uniform, transparent, mild and clean base solution. Deodorization and buffer system establishment: Slowly add 2.0% hydroxypropyl-β-cyclodextrin to the base solution obtained in step 1 and stir for 30 minutes to completely dissolve it; Separately, dissolve 0.8% sodium bicarbonate in 50mL of deionized water, filter and add it to the main tank, then add 0.2% sodium citrate buffer solution to stabilize the pH of the system between 6.8 and 7.

0. Activation of the bio-enzyme system: Cool the system to below 25°C, add 0.6% of the total content of the compound enzyme, and simultaneously add 0.2% of the enzyme stabilizer. Stir for 15 minutes while avoiding the generation of bubbles, and then test the transparency and enzyme activity. Addition of antibacterial and preservative system: Add 0.2% silver citrate solution under light-protected conditions and stir gently for 10 minutes; then add 0.25% phenoxyethanol and 0.15% ethylhexylglycerin in sequence, stir and mix evenly, and check that the pH of the system at this time should be 6.4-6.6; Structure and stability control: 0.3% hydroxyethyl cellulose was slowly and evenly added under stirring at 400 rpm, and stirring was continued for 40 minutes until completely dissolved, and the viscosity of the system was stable; then it was allowed to stand to degas or vacuum degassed (-0.08 MPa), and filtered through a 0.45 μm filter membrane to ensure transparency ≥95%; Final adjustment and filling: The pH of the system is finely adjusted to 6.5±0.2 using citric acid or sodium hydroxide solution. After final filtration through a 0.22μm filter membrane, the system is filled at 22±2℃ and packaged in light-proof PET or HDPE bottles with leak-proof nozzles. Quality control and sample retention: Each batch of product is tested for appearance, pH, viscosity, silver ion content, microbial indicators and enzyme activity; at least 200 mL of each batch is retained and sealed for 12 months for stability traceability. Storage conditions: The finished product should be stored in a dark, dry environment at a temperature ≤25℃. The shelf life is 18 months in the unopened state.

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