Preparation method and application of sensitive skin repairing composition containing phellinus linteus PDRN

Abstract

The invention relates to a preparation method and application of a sensitive skin repairing composition containing phellinus igniarius PDRN, and belongs to the technical field of cosmetics. The sensitive skin repairing composition is prepared from phellinus medicinus PDRN, bio-saccharide gum-1 and tephrosia esculenta seed extract, and the three components have a synergistic effect and can effectively promote the generation of three barrier proteins, namely tight junction protein, biaprylin and fibroin, so that the skin barrier function is strengthened, the long-acting repairing of sensitive skin is realized, and the skin sensitivity is reduced.

Description

Technical Field

[0001] This invention belongs to the field of cosmetic technology and relates to a method for preparing a sensitive skin repair composition containing medicinal Fomitopsis pilosula PDRN and its application. Background Technology

[0002] Polydeoxyribonucleotide (PDRN) is a biologically active DNA fragment, typically extracted from specific biological sources. It has demonstrated significant application potential in the fields of aesthetic medicine and skincare, with main functions including promoting cell repair and regeneration, anti-inflammatory and soothing effects, and stimulating collagen synthesis. Therefore, it is widely used in post-operative repair, skin barrier strengthening, and anti-aging. Early PDRN was primarily used in aesthetic medicine via injection (such as mesotherapy) to directly target the dermis. In recent years, with advancements in formulation technology, topical skincare products have also begun to incorporate it, utilizing nano-encapsulation and fragmentation techniques to enhance transdermal absorption efficiency, thereby repairing damaged skin, improving sensitivity, and delaying photoaging.

[0003] Currently, commercially available PDRN sources are mainly divided into animal-derived, plant-derived, and microbial-derived sources. Animal-derived PDRN (such as that derived from salmon seminal vesicles) is the traditional mainstream, but it faces challenges such as the supply of raw materials being constrained by the reproductive cycle and marine environment, potential allergenic risks, and ethical controversies. To overcome these limitations, the industry has gradually developed PDRN from plant sources (such as Gynostemma pentaphyllum and rose) and microbial fermentation sources (such as Lactobacillus). While these new sources have improved sustainability and safety to some extent, they are still limited by factors such as plant growth cycles, extraction purity, or fermentation process stability, and the consistency of their molecular structure and biological activity needs further improvement. Summary of the Invention

[0004] The purpose of this invention is to provide a method for preparing a sensitive skin repair composition containing medicinal dermal PDRN and its application. The sensitive skin repair composition includes medicinal dermal PDRN, biosaccharide gum-1, and edamame seed extract. The three components work synergistically to effectively promote the generation of three major barrier proteins: tight junction protein, lipogrin, and filaggrin, thereby strengthening the skin barrier function, achieving long-term repair of sensitive skin, and reducing skin sensitivity.

[0005] The objective of this invention can be achieved through the following technical solutions: In a first aspect, the present invention provides a sensitive skin repair composition containing medicinal dermal PDRN, the composition comprising the following components: medicinal dermal PDRN, biosaccharide gum-1, and grey soybean seed extract, wherein the weight ratio of medicinal dermal PDRN, biosaccharide gum-1, and grey soybean seed extract is (0.01-1):(0.01-2):(0.01-3).

[0006] Preferably, the weight ratio of the medicinal dermal PDRN, biosaccharide gum-1, and soybean seed extract is (0.05-0.5):(0.1-0.5):(0.1-2).

[0007] Preferably, the weight ratio of the medicinal dermal PDRN, biosaccharide gum-1, and soybean seed extract is (0.1-0.2):(0.2-0.3):(0.5-1).

[0008] Secondly, the present invention provides a method for preparing a sensitive skin repair composition, comprising the following steps: The medicinal dermal PDRN, biosaccharide gum-1, and edamame seed extract were mixed evenly in a certain weight ratio to obtain a sensitive skin repair composition.

[0009] Thirdly, the present invention provides the application of the sensitive skin repair composition of the second aspect in the preparation of skin care products, the skin care products including toners, lotions, creams, masks, and sprays.

[0010] Fourthly, the present invention provides an emulsion comprising the following ingredients by weight percentage: 0.5%-2% of the sensitive skin repair composition containing medicinal Fomitopsis pilosula PDRN as described in claim 1, 0.05%-0.45% thickener, 1%-6% moisturizer, 0.01%-0.3% pH adjuster, 0.2%-5% emulsifier, 1%-8% oil, 0.05%-1.5% preservative, and the balance being deionized water.

[0011] Preferably, the thickener comprises at least one of the following: polyacrylate crosspolymer-6, carbomer, carrageenan, gellan gum, xanthan gum, microcrystalline cellulose, cellulose gum, ethyl cellulose, sclerotium taurate gum, guar gum, ammonium acryloyl dimethyl taurate / VP copolymer, acrylate copolymers, and sclerotium taurate gum; Preferably, the moisturizer includes at least one of glycerin, 1,2-butanediol, 1,3-butanediol, 1,2-hexanediol, 1,3-propanediol, caprylyl glycol, dipropylene glycol, tremella polysaccharide, trehalose, betaine, allantoin, sodium hyaluronate, β-glucan, budding stalk polysaccharide, and ceramide. Preferably, the pH adjuster includes at least one of arginine, triethanolamine, and potassium phosphate; Preferably, the emulsifier comprises at least one of C14-22 alcohol, C12-20 alkyl glucoside, polysorbate-20, polysorbate-60, cetearyl glucoside, cetearyl alcohol polyether-20, polyglycerol-3 diisostearate, sodium lauroyl lactylate, and PEG-40 hydrogenated castor oil; Preferably, the oil comprises at least one of caprylic / capric triglyceride, jojoba oil, olive oil, cetyl ethylhexanoate, isooctyl palmitate, triethylhexanoate, tricaprylic triethyl ester, squalane, and cetearyl alcohol. Preferably, the preservative includes at least one of 1,3-propanediol, 1,2-hexanediol, and p-hydroxyacetophenone.

[0012] The beneficial effects of this invention are: The sensitive skin repair composition provided by this invention contains medicinal F. pylori PDRN, biosaccharide gum-1, and soybean seed extract. PDRN is a small-molecule polydeoxyribonucleotide derived from F. pylori, which accelerates skin barrier repair by activating cellular energy metabolism and promoting barrier protein production. Biosaccharide gum-1 is a biopolysaccharide derived from brown algae, which soothes and provides long-lasting hydration by inhibiting macrophage migration inhibitory factor (MIF) and forming a breathable protective film on the skin surface. Soothing and anti-allergic effects are achieved through long-lasting moisturizing. Soybean seed extract is rich in polysaccharides and flavonoids, which soothe the skin and improve skin barrier function by activating stress-regulating factors and enhancing skin metabolism. These three components work synergistically to effectively promote the production of three major barrier proteins: tight junction proteins, laminarin, and filaggrin, thereby strengthening the skin barrier function, achieving long-lasting repair of sensitive skin, and reducing skin sensitivity. Detailed Implementation

[0013] To further illustrate the technical means and effects of the present invention in achieving its intended purpose, the following detailed description of the specific implementation methods, structures, features, and effects of the present invention, in conjunction with embodiments, is provided below.

[0014] Biosaccharide Gum-1 is from Solabia, France, and is marketed under the name FUCOGEL® POWDER. Biosaccharide Gum-2 is from Solabia, France, and is marketed under the name Rhamnosoft®HP. The edamame seed extract is from Givaudan, Switzerland, and is marketed under the brand name Sensityl™. The soybean extract was purchased from Shanxi Yuqu Biotechnology Co., Ltd. The salmon PDRN was purchased from Shanghai Saimengxin Biotechnology Co., Ltd. The preparation method of medicinal Fomitopsis pilosula PDRN includes the following steps: (1) Pulverization: Take the dried fruiting body of medicinal fumonis (purchased from Anhui Boman Pharmaceutical Co., Ltd.), pulverize it and pass it through an 80-mesh sieve to obtain medicinal fumonis powder; (2) Extraction: Add medicinal fuciformis powder to Tris-HCl buffer solution with pH 8.0 and 2% g / mL PVP at a material-to-liquid ratio of 1g:20mL. Extract by ultrasonication for 40 min at a power of 500W and a temperature of 60℃. Centrifuge and collect the supernatant. (3) Removal of impurities: Add RNase to the supernatant to a final concentration of 10 U / mL and react at 37°C for 30 min to remove RNA impurities. Then add protease to a final concentration of 10 U / mL and react at 37°C for 30 min to remove protein impurities. Centrifuge and take the supernatant. (4) Precipitation: Add 3 times the volume of anhydrous ethanol to the supernatant to precipitate the DNA. Collect the precipitate and wash it with anhydrous ethanol to remove impurities. Centrifuge to collect the precipitate and dry it to obtain medicinal PDRN of Phytophoresis.

[0015] A method for preparing medicinal Fomitopsis thunbergii extract includes the following steps: The dried fruiting bodies of *Polyporus medica* (purchased from Anhui Boman Pharmaceutical Co., Ltd.) were pulverized and passed through an 80-mesh sieve. The *Polyporus medica* powder was added to a 70% ethanol aqueous solution at a material-to-liquid ratio of 1g:20mL. The mixture was ultrasonically extracted for 40 min at a power of 500W and a temperature of 60℃. The supernatant was collected by centrifugation, concentrated, and dried to obtain the *Polyporus medica* extract.

[0016] Unless otherwise specified, all other materials, reagents, etc. used in all embodiments and comparative examples of this invention are commercially available.

[0017] The components and their weight ratios of the repair compositions of Examples 1-6 and Comparative Examples 1-5 are shown in Table 1, and the total weight of each composition is equal. Table 1. Components and weight ratios of the repair composition Group Medicinal Fomitopsis pilosula PDRN Biosaccharide Gum-1 Edamame seed extract Example 1 0.2 0.3 0.5 Example 2 0.1 0.2 1 Example 3 0.5 0.5 0.1 Example 4 0.05 0.1 2 Example 5 1 0.01 3 Example 6 0.01 2 0.01 Comparative Example 1 2 3 0.005 Comparative Example 2 0.005 0.005 5 Comparative Example 3 / 0.3 0.5 Comparative Example 4 0.2 / 0.5 Comparative Example 5 0.2 0.3 / The method for preparing the repair composition provided in Example 1 of this application includes the following steps: The medicinal dermal PDRN, biosaccharide gum-1, and edamame seed extract were mixed evenly in a specific weight ratio to obtain a repair composition.

[0018] The preparation methods of the repair compositions provided in Examples 2-6 and Comparative Examples 1-5 are consistent with those in Example 1.

[0019] If the comparative formulation does not contain any of the following: medicinal dermal PDRN, biosaccharide gum-1, and grey soybean seed extract, then the preparation method does not include them.

[0020] Comparative Example 6 The only difference between this comparative example and Example 1 is that this comparative example uses salmon PDRN instead of medicinal PDRN.

[0021] Comparative Example 7 The only difference between this comparative example and Example 1 is that this comparative example uses medicinal blastocyst extract instead of medicinal blastocyst PDRN.

[0022] Comparative Example 8 The only difference between this comparative example and Example 1 is that this comparative example uses biosaccharide gum-2 instead of biosaccharide gum-1.

[0023] Comparative Example 9 The only difference between this comparative example and Example 1 is that this comparative example uses soybean extract instead of edamame seed extract.

[0024] Test Example: Repair Effect Test This test case investigates the repair effects of the repair compositions prepared in Examples 1-6 and Comparative Examples 1-9.

[0025] Experimental materials: The cell line used was human keratinocytes (HaCaT, Beina Biotechnology). The testing conditions were: incubator temperature 37±1℃, humidity 90±5%, carbon dioxide 5±1%. Cells were cultured and treated according to groups, followed by testing. Specifically, the content of tight junction proteins, lipogrin, and filaggrin was detected using the following methods: (1) Seed the cell suspension into a 96-well cell culture plate at a density of 2000 cells / well, add 100 μL of culture medium to each well, and after culturing for 24 hours, discard the supernatant; (2) 100 μL of DMEM medium (2105341, Gibco) was added to the blank control group, and 100 μL of DMEM medium containing 0.1% g / mL repair composition was added to the sample group. After culturing for 24 h, the cells were collected, washed 3 times with PBS buffer, and 50 μL of RIPA cell lysis buffer containing protease inhibitor was added to each well. The lysed samples were centrifuged at 12000 g for 5 minutes and the supernatant was collected. (3) Determination of tight junction protein content: Follow the instructions of the human tight junction protein 1 (ZO-1) ELISA kit (catalog number ml058798, Shanghai Enzyme-Linked Biotechnology). Take equal amounts of cell culture supernatant from the blank control group and the sample group, add them to the corresponding reaction wells of the kit, and after incubation, washing, and color development, measure the absorbance value at a specific wavelength using an ELISA reader. Calculate the content of tight junction protein in each group based on the standard curve. (4) Determination of apigenin content: Follow the instructions of the human apigenin (LOR) ELISA kit (catalog number ml105108, Shanghai Enzyme-Linked Biotechnology). Take equal amounts of cell culture supernatant from the blank control group and the sample group, add them to the corresponding reaction wells of the kit, and after incubation, washing, and color development, measure the absorbance value at a specific wavelength using an ELISA reader. Calculate the apigenin content of each group based on the standard curve. (5) Determination of filaggrin content: Follow the instructions of the human filaggrin (FLG) ELISA kit (catalog number ml057769, Shanghai Enzyme-Linked Biotechnology). Take equal amounts of cell culture supernatant from the blank control group and the sample group, and add them to the corresponding reaction wells of the kit. After incubation, washing, and color development, use an ELISA reader to measure the absorbance value at a specific wavelength. Calculate the filaggrin content of each group according to the standard curve.

[0026] Tight junction proteins, nautiloids, and filaggrins are key functional proteins that constitute the skin's physical barrier. They work synergistically to maintain skin health and stability. Tight junction proteins are mainly distributed between keratinocytes in the granular layer of the epidermis, forming a selective barrier like an "intercellular seal," effectively preventing the loss of moisture and electrolytes through intercellular spaces and defending against the invasion of external allergens and microorganisms. A decline in their function can lead to excessive extension of nerve fibers to the skin surface, causing discomfort such as stinging and burning. Nautiloids are the main component of the "keratinized capsule" of keratinocytes. Like the cell's "internal armor," they are extensively cross-linked with other proteins under the action of transglutaminase, forming a strong and elastic structure, thereby enhancing the mechanical strength of keratinocytes and their resistance to environmental stimuli. Filaggrins are converted into natural moisturizing factors in the granular layer. These components effectively lock in moisture, maintaining normal hydration and a slightly acidic environment in the stratum corneum. Simultaneously, filaggrins assist keratin in aggregation into bundles, which is crucial for the formation of a dense, smooth, scaly structure in the stratum corneum. Defects in filaggrins directly weaken the skin's moisturizing ability and barrier integrity. These three functions complement each other, and any abnormality in any link may damage the skin barrier, causing problems such as dryness, sensitivity, or even atopic dermatitis.

[0027] Therefore, the higher the content of tight junction proteins, lipofuscin, and filaggrin after sample treatment, the better the skin barrier function and the better the repair effect of the composition. The repair effect of the repair composition is expressed by the increase rate of tight junction proteins, lipofuscin, and filaggrin, as shown in the following formula: Increase rate = (sample group / blank group - 1) × 100%; The test results are shown in Table 2.

[0028] Table 2. Repair Effect Test Results Group Increase in tight junction protein percentage Increase rate of lipoprotein / % Increase rate of filaggrin / % Example 1 53.1 76.4 79.6 Example 2 48.7 68.2 72.1 Example 3 42.3 58.9 65.4 Example 4 38.7 53.2 60.8 Example 5 35.5 50.1 51.7 Example 6 36.8 52.4 55.2 Comparative Example 1 25.3 39.8 38.5 Comparative Example 2 22.1 36.2 35.7 Comparative Example 3 10.8 18.6 21.4 Comparative Example 4 12.5 15.3 20.8 Comparative Example 5 11.2 16.9 19.5 Comparative Example 6 18.7 32.5 28.4 Comparative Example 7 14.2 24.8 25.1 Comparative Example 8 13.8 22.7 23.9 Comparative Example 9 15.1 26.3 24.5 As shown in Table 2, compared with Comparative Examples 1-9, the sensitive skin repair compositions provided in Examples 1-6 can effectively promote the generation of the three major barrier proteins: tight junction protein, lipoprotein, and filaggrin, thereby strengthening the skin barrier function, achieving long-term repair of sensitive skin, and reducing skin sensitivity.

[0029] The results of comparing Examples 1-6 and Comparative Examples 1-2 show that the dosage ratio of *Polyporus medica* PDRN, biosaccharide gum-1, and *Edamame oleracea* seed extract affects the repair ability of the composition. When the weight ratio of *Polyporus medica* PDRN, biosaccharide gum-1, and *Edamame oleracea* seed extract is (0.01-1):(0.01-2):(0.01-3), the components have a synergistic effect, and the repair effect of the composition is better. The results of comparing Examples 1 and Comparative Examples 3-5 show that *Polyporus medica* PDRN, biosaccharide gum-1, and *Edamame oleracea* seed extract have a synergistic effect, and the absence of any one of the components will lead to a decrease in the repair effect of the composition. The results of Examples 1 and Comparative Examples 6-9 show that the sensitive skin repair composition of the present invention has irreplaceable components. Any substitution of the components may lead to the final repair effect failing to meet the expectations of the present invention.

[0030] Application Examples 1-8, Comparison of Application Examples 1-9 and Blank Application Examples This application provides an emulsion in application examples, comparative application examples, and blank application examples, the components (mass percentage) of which are shown in Table 3; wherein, the repair compositions used in application examples 1-6 are the repair compositions prepared in examples 1-6 respectively, the repair compositions used in comparative application examples 1-9 are the repair compositions prepared in comparative examples 1-9 respectively; and the repair compositions used in application examples 7-8 are the repair compositions prepared in example 1.

[0031] The preparation methods of the emulsions in Application Examples 1-8, Comparative Application Examples 1-9, and the blank application example specifically include the following steps: S1. Mix the humectant, thickener and deionized water, heat to 85°C, and homogenize at 1300 rpm for 4 min. After homogenization, keep warm at 80°C for later use to obtain the pre-prepared phase A. S2. Mix the emulsifier and oil, heat to 75°C, homogenize at 1300 rpm for 4 minutes, and keep warm at 80°C for later use to obtain the pre-prepared phase B. S3. Mix the preservatives and heat to 60°C to melt them, to obtain the pre-prepared C phase; S4. Add the pre-prepared phase B to the pre-prepared phase A at 300 rpm, stir and mix well, cool to 60°C, then add the pre-prepared phase C at 300 rpm and stir and mix well, cool to 45°C, add the repair composition, continue stirring at 300 rpm for 8 minutes, add the pH adjuster, stir evenly, and discharge to obtain the emulsion.

[0032] Table 3 Emulsion Formulation

[0033] Effect test: 1. Human skin patch test Thirty volunteers were recruited, 15 men and 15 women, aged 20-50 years. A closed patch test method was used. Equal volumes (0.020 mL-0.025 mL) of test samples (emulsions prepared in Application Examples 1-8, Control Examples 1-9, and the blank application example) were placed in specific patch applicators. These were then applied to the volunteers' arms with hypoallergenic adhesive tape, gently pressed to ensure even adhesion to the skin, and left for 24 hours. The blank control group consisted of deionized water. One patch applicator was applied to each arm of each volunteer. Each patch applicator had 10 wells, allowing for the simultaneous testing of 18 samples per volunteer (both arms). After 24 hours, the patch applicators were removed, and skin reactions were observed and recorded at 0.5 hours, 24 hours, and 48 hours. The severity of adverse skin reactions is shown in Table 4 below.

[0034] Table 4 Skin Reaction Grading Standards Rating levels Skin reaction 0 negative reaction 1 Suspicious reaction, only slight erythema 2 Weak positive reaction (erythema reaction): erythema, infiltration, edema, and papules may be present. 3 Strong positive reaction (herpes reaction): erythema, infiltration, edema, papules; the reaction may extend beyond the test area. 4 Extremely strong positive reaction (confluent herpes simplex reaction): obvious erythema, severe infiltration, edema, confluent herpes simplex; reaction extends beyond the test area. After testing, the lotions provided in Application Examples 1-8, Comparative Application Examples 1-9, and Blank Application Examples of the present invention all showed negative reactions after human patch testing, indicating that they are safe and non-irritating to human skin.

[0035] 2. Human efficacy testing of the compound for repair and soothing. Experimental Methods: 108 Asian adults aged 18-60 years with self-reported skin sensitivity were randomly divided into 18 groups of 6 participants each. Volunteers applied 1 mL of the sample to their entire face twice daily (morning and evening) (samples were the lotions provided in Application Examples 1-8, Control Examples 1-9, and the blank application example). Data were collected before application (T0) and 14 days after application (T14). After washing their faces with facial cleanser upon arrival, volunteers sat quietly for 30 minutes in an air-conditioned room at 21±1℃ and 50±10% humidity. Researchers measured the transcutaneous water loss (TEWL) value of the cheekbone using a TewaMeter®™ Hex probe and measured the skin current sensory threshold (CPT, 5Hz) of the cheek using a sensory nerve quantitative detector (NEUROMETER CPT / C).

[0036] Skin barrier repair capacity is represented by the improvement in TEWL value, and skin soothing capacity is represented by the improvement in CPT value, as shown in the following formula: TEWL value improvement rate = (sample) T0 -sample T14 ) / sample T0×100%; CPT value improvement rate = (sample) T14 -sample T0 ) / sample T0×100%; The results are shown in Table 5.

[0037] Table 5 Repairing and Soothing Effects TEWL value improvement rate (%) CPT value improvement rate (%) Blank application example 0.3 2.0 Application Example 1 15.3 88.5 Application Example 2 14.7 86.3 Application Example 3 12.1 67.8 Application Example 4 10.5 61.5 Application Example 5 9.3 58.4 Application Example 6 8.0 51.2 Application Example 7 8.6 55.5 Application Example 8 15.6 88.9 Comparative Application Example 1 6.0 38.5 Comparative Application Example 2 5.5 35.2 Comparative Application Example 3 4.5 30.8 Comparative Application Example 4 3.8 27.3 Comparative Application Example 5 3.2 25.0 Comparative Application Example 6 3.5 26.4 Comparative Application Example 7 1.8 15.3 Comparative Application Example 8 1.5 12.1 Comparative Application Example 9 1.2 10.5 As shown in Table 5, the emulsion prepared using the repair composition of the present invention can effectively repair the skin barrier and soothe skin sensitivity. It can be applied to people with sensitive skin and has a good repair effect.

[0038] The above description is merely a preferred embodiment of the present invention and is not intended to limit the present invention in any way. Although the present invention has been disclosed above with reference to preferred embodiments, it is not intended to limit the present invention. Any person skilled in the art can make some modifications or alterations to the above-disclosed technical content to create equivalent embodiments without departing from the scope of the present invention. Any indirect modifications, equivalent changes, and alterations made to the above embodiments based on the technical essence of the present invention without departing from the scope of the present invention shall still fall within the scope of the present invention.

Claims

1. A sensitive skin repair composition containing medicinal Fomitopsis pilosula PDRN, characterized in that, The composition comprises the following components: medicinal dermal PDRN, biosaccharide gum-1, and grey soybean seed extract, wherein the weight ratio of medicinal dermal PDRN, biosaccharide gum-1, and grey soybean seed extract is (0.01-1):(0.01-2):(0.01-3).

2. The sensitive skin repair composition containing medicinal Fomitopsis pilosula PDRN according to claim 1, characterized in that, The weight ratio of the medicinal Fomitopsis pilosula PDRN, biosaccharide gum-1, and soybean seed extract is (0.05-0.5):(0.1-0.5):(0.1-2).

3. The sensitive skin repair composition containing medicinal Fomitopsis pilosula PDRN according to claim 1, characterized in that, The weight ratio of the medicinal Fomitopsis pilosula PDRN, biosaccharide gum-1, and soybean seed extract is (0.1-0.2):(0.2-0.3):(0.5-1).

4. The method for preparing the sensitive skin repair composition containing medicinal dermal PDRN according to any one of claims 1-3, characterized in that, Includes the following steps: The medicinal dermal PDRN, biosaccharide gum-1, and edamame seed extract were mixed evenly in a certain weight ratio to obtain a sensitive skin repair composition.

5. The application of the sensitive skin repair composition prepared by the method according to claim 4 in the preparation of skin care products, characterized in that, The skincare products include toners, lotions, creams, masks, and sprays.

6. An emulsion, characterized in that, The emulsion comprises the following raw materials by weight percentage: 0.5%-2% of the sensitive skin repair composition containing medicinal Fomitopsis pilosula PDRN as described in claim 1, 0.05%-0.45% thickener, 1%-6% moisturizer, 0.01%-0.3% pH adjuster, 0.2%-5% emulsifier, 1%-8% oil, 0.05%-1.5% preservative, and the balance being deionized water.

7. The emulsion according to claim 6, characterized in that, The raw material is selected from at least one of (a)-(f): (a) The thickener comprises at least one of the following: polyacrylate crosspolymer-6, carbomer, carrageenan, gellan gum, xanthan gum, microcrystalline cellulose, cellulose gum, ethyl cellulose, sclerotium taurate gum, guar gum, ammonium acryloyl dimethyl taurate / VP copolymer, acrylate copolymer, and sclerotium taurate gum; (b) The moisturizer comprises at least one of the following: glycerin, 1,2-butanediol, 1,3-butanediol, 1,2-hexanediol, 1,3-propanediol, caprylyl glycol, dipropylene glycol, tremella polysaccharide, trehalose, betaine, allantoin, sodium hyaluronate, β-glucan, budding stalk polysaccharide, and ceramide. (c) The pH adjuster includes at least one of arginine, triethanolamine, and potassium phosphate; (d) The emulsifier comprises at least one of C14-22 alcohol, C12-20 alkyl glucoside, polysorbate-20, polysorbate-60, cetearyl glucoside, cetearyl alcohol polyether-20, polyglycerol-3 diisostearate, sodium lauroyl lactylate, and PEG-40 hydrogenated castor oil; (e) The oils include at least one of caprylic / capric triglyceride, jojoba oil, olive oil, cetyl ethylhexanoate, isooctyl palmitate, triethylhexanoate, tricaprylic triethyl ester, squalane, and cetearyl alcohol; (f) The preservative includes at least one of 1,3-propanediol, 1,2-hexanediol, and p-hydroxyacetophenone.

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