Long-acting hair care composition containing silk fibroin peptide and application thereof

Abstract

The application belongs to the technical field of daily chemicals, and particularly relates to a long-acting hair quality repairing composition containing silk fibroin peptides and application thereof. The application discloses a long-acting hair quality repairing composition containing silk fibroin peptides, which comprises a silk fermentation repairing liquid, silk fibroin peptides and a silk fibroin surfactant. The three components can synergistically promote the combing performance, anti-static performance and tensile performance of the composition. The silk fermentation repairing liquid is prepared by inoculating Saccharomyces cerevisiae and Rhodotorula glutinis in a fermentation medium containing silk glue proteins, then carrying out mixed fermentation, and finally carrying out enzymatic hydrolysis. The silk fermentation repairing liquid can effectively improve the damage problems of dry, rough and broken hair. The long-acting hair quality repairing composition containing silk fibroin peptides can be applied to the preparation of hair care products such as hair conditioner, shampoo, hair mask and hair care essential oil. The long-acting hair quality repairing composition containing silk fibroin peptides is safe and non-irritating, can realize long-acting and multi-dimensional repair of damaged hair quality, and has the effects of softness, gloss and repair.

Description

A composition containing silk protein peptides for long-lasting hair repair and its application. Technical Field

[0001] This invention belongs to the field of daily chemical products technology, specifically relating to a composition containing silk protein peptides for long-lasting hair repair and its application. Background Technology

[0002] Hair is often damaged by a variety of factors, including rinsing, drying, heating, combing, styling, perming, dyeing, and exposure to various natural environments, leading to a range of problems such as keratin loss, dryness, frizz, tangles, breakage, and hair loss. With people's increasing pursuit of beauty, these issues have become a source of distress for many.

[0003] Currently, there are many hair care products available. Some hair care products, by adding silicone oil and silicone oil emulsion, have excellent repairing and nourishing effects, making hair smooth and tangle-free. However, long-term use of silicone oil can lead to its accumulation on the hair roots and scalp, causing clogged hair follicles and resulting in scalp itching, increased dandruff, folliculitis, and worsening of seborrheic dermatitis. Other silicone-free hair care products add herbal extracts to repair hair while nourishing the hair roots and scalp, resulting in smoother, more lubricated hair and alleviating scalp allergy symptoms. However, these products have higher production costs and are limited to rinsing, making it difficult for the effective nourishing ingredients to be absorbed promptly.

[0004] In summary, providing a hair care product that can further promote the absorption of nutrients, thereby achieving better repair effects, without side effects and with long-lasting results, has become an urgent problem to be solved. Summary of the Invention

[0005] In view of the above-mentioned defects or improvement needs of the existing technology, the present invention provides a composition containing silk protein peptides for long-lasting hair repair and its application, which solves the problems of slow absorption and side effects of existing products.

[0006] To achieve the above objectives, the present invention adopts the following technical solution:

[0007] In a first aspect, the present invention provides a composition for long-lasting hair repair containing silk protein peptides, the composition comprising the following components by weight: 5-10 parts of silk fermentation repair liquid, 4-9 parts of silk peptides, and 12-17 parts of silk protein surfactant.

[0008] The preparation method of the silk fermentation repair solution includes the following steps:

[0009] A1. Inoculate *Saccharomyces cerevisiae* and *Rhodotorula glutinis* into a sterilized fermentation medium containing sericin and ferment to obtain the fermentation product; wherein, the fermentation medium containing sericin contains the following components by mass percentage: 2%-4% sericin, 0.2%-0.5% lactose, 0.05%-0.1% KH2PO4, 0.05%-0.1% MgSO4·7H2O, and the balance is deionized water; the preservation number of *Saccharomyces cerevisiae* is GDMCC NO.2.121, and the preservation number of *Rhodotorula glutinis* is GDMCC NO.2.224;

[0010] A2. Papain is added to the fermentation product for enzymatic hydrolysis. After complete hydrolysis, the enzyme is inactivated by heating to obtain the hydrolysate. The amount of papain added is 2-3 wt% of the fermentation product, the enzyme activity of the papain is 800-1000 U / mg, the hydrolysis temperature is 50-60℃ and the time is 30-45 min, and the enzyme inactivation temperature is 85-95℃ and the time is 15-25 min.

[0011] A3. After adding activated carbon to the enzymatic hydrolysate for adsorption and decolorization, centrifuge, take the supernatant, filter and sterilize, add butanediol and p-hydroxyacetophenone to the sterilized filtrate, mix well, and obtain the silk fermentation repair solution.

[0012] Preferably, the silk fibroin peptide comprises the following components by mass percentage: 4%-10% hydrolyzed silk, 0.2%-0.8% phenoxyethanol, and the balance being deionized water.

[0013] Preferably, the silk protein surfactant is lauroyl hydrolyzed silk sodium.

[0014] The sterilized filtrate from the silk fermentation repair solution is fermented with brewer's yeast and Rhizopus cylindrica, and hydrolyzed with papain to break down large-molecule sericin into smaller peptides that are easier to penetrate. This process promotes absorption, reduces inflammation, regulates the microecology, and strengthens the scalp barrier. Furthermore, the yeast fermentation metabolites contain active ingredients such as amino acids, organic acids, and polysaccharides, which further enhance the moisturizing properties, penetration, bioavailability, and repair of damaged hair.

[0015] The amino acid composition of hydrolyzed silk in silk peptides is similar to that of hair proteins lost due to damage, making it very suitable for hair care. It has good water solubility and permeability, allowing it to penetrate into the hair and repair damaged hair. At the same time, it has excellent film-forming properties, which can increase hair elasticity, softness and moisturization, making the hair shiny and easy to comb.

[0016] Sodium lauroyl hydrolyzed silk has excellent moisturizing, biocompatibility, and antistatic properties, and can repair damaged hair and increase its moisture content.

[0017] More preferably, the composition comprises the following components by weight: 7 parts silk fermentation repair liquid, 6 parts silk fibroin peptide, and 14 parts silk protein surfactant.

[0018] More preferably, in step A1, the total inoculation amount of Saccharomyces cerevisiae and Rhodotorula glutinis is 1-3% v / v of the fermentation medium volume.

[0019] More preferably, the total viable count of the *Saccharomyces cerevisiae* and *Rhodotorula glutinis* is 7.0 × 10⁻⁶. 8 -3.0×10 9 CFU / mL.

[0020] More preferably, the ratio of live bacteria of Saccharomyces cerevisiae and Rhodotorula glutinis in step A1 is 1.7-2.2:1.

[0021] More preferably, in step A1, the ratio of live Saccharomyces cerevisiae to Rhodotorula glutinis is 2:1.

[0022] More preferably, the fermentation temperature in step A1 is 25-30℃ and the time is 36-48h.

[0023] More preferably, in step A3, the amount of butanediol added is 10-15% and the amount of p-hydroxyacetophenone added is 0.3-0.5% based on the mass of the sterilized filtrate.

[0024] Secondly, the present invention provides the use of the composition described in the first aspect in the preparation of shampoo and conditioner products with long-lasting hair repair effects.

[0025] Preferably, the hair care products include shampoo, hair serum, hair oil, hair conditioner, hair mask, and hair treatment.

[0026] Thirdly, the present invention provides a hair conditioner comprising the following raw materials in weight percentages: 0.1%-2% of the composition described in the first aspect, 5%-10% of a humectant, 3%-8% of a thickener, 2%-6% of an emulsifier, 0.5%-1.5% of a conditioning agent, 0.1%-1.5% of a preservative, and the balance being deionized water.

[0027] Preferably, the moisturizer is selected from at least one of glycerin, butylene glycol, propylene glycol, betaine, sodium hyaluronate, and panthenol.

[0028] Preferably, the thickener is selected from at least one of hydroxypropyl methylcellulose, carbomer, xanthan gum, and cetearyl alcohol.

[0029] Preferably, the emulsifier is selected from at least one of glyceryl stearate, oleyl alcohol polyether-10, PEG-100 stearate, PEG-10 polydimethylsiloxane, cetearyl glucoside, sodium stearoyl lactylate, and sodium stearoyl glutamate.

[0030] Preferably, the conditioner is selected from at least one of behenyltrimethylammonium chloride, behenyltrimethylammonium methyl sulfate, behenamidopropyl dimethylamine, stearamidopropyl dimethylamine, stearyltrimethylammonium chloride, and stearyltrimethylammonium methyl sulfate.

[0031] Preferably, the preservative is selected from at least one of phenoxyethanol, sodium benzoate, potassium sorbate, and p-hydroxyacetophenone.

[0032] Fourthly, the present invention provides a method for preparing the hair conditioner described in the third aspect, comprising the following steps:

[0033] S1. Mix the humectant, thickener, emulsifier and deionized water, and stir at 150-250 rpm to obtain a mixture.

[0034] S2. Add the conditioner, preservative and long-lasting hair repairing components to the mixture, and stir at 250-350 rpm to obtain the hair conditioner.

[0035] The beneficial effects of this invention are:

[0036] This invention provides a long-lasting hair repair composition containing silk fibroin peptides. The composition comprises a silk fermentation repair liquid, silk fibroin peptides, and silk fibroin surfactants. The three components work synergistically, resulting in a composition with excellent combing properties, antistatic properties, and tensile strength. The silk fermentation repair liquid is prepared by inoculating a fermentation medium containing silk fibroin with *Saccharomyces cerevisiae* and *Rhodotorula glutinis* for mixed fermentation followed by enzymatic hydrolysis. The silk fibroin is hydrolyzed into small molecule active substances such as polypeptides, serine, and aspartic acid. This preparation process improves the permeability and bioavailability of the active substances, promoting efficient absorption of active substances by the hair. Simultaneously, the fermentation metabolites of *Saccharomyces cerevisiae* and *Rhodotorula glutinis* contain natural active substances such as polysaccharides, which can act on damaged hair, effectively improving dryness, frizz, breakage, and other damage problems.

[0037] Applying the long-lasting hair repair composition of this invention to the preparation of hair care products such as conditioners is not only safe and non-irritating, but also enables long-lasting, multi-dimensional repair of damaged hair, resulting in smoothness, shine, and repair effects. Detailed Implementation

[0038] To further illustrate the technical means and effects of the present invention in achieving the intended purpose, the following detailed description of the specific embodiments, structure, features and effects of the present invention, in conjunction with the composition, is provided below.

[0039] For the following compositions, unless otherwise specified, experimental methods are generally performed under standard conditions or as recommended by the manufacturer. Unless otherwise specified, all materials and reagents used are commercially available.

[0040] Some of the raw materials and their sources are as follows:

[0041] The silk fibroin was purchased from Huzhou Anran Biotechnology Co., Ltd.

[0042] Hydrolyzed silk was purchased from Huzhou Anran Biotechnology Co., Ltd.

[0043] Sodium lauroyl hydrolyzed silk was purchased from Suzhou Aoshi Biotechnology Co., Ltd.

[0044] Papain was purchased from Shanghai Yuanye Biotechnology Co., Ltd., with an enzyme activity of 800 U / mg.

[0045] Silk Fermented Repair Solution ①

[0046] Its preparation method includes the following steps:

[0047] A1. Inoculate Saccharomyces cerevisiae and Rhodotorula buergerianum into a sterilized fermentation medium containing silk fibroin for fermentation to obtain fermentation products;

[0048] A2. Add papain to the fermentation product for enzymatic hydrolysis. After complete hydrolysis, heat to inactivate the enzyme and obtain the hydrolysate.

[0049] A3. After adding activated carbon to the enzymatic hydrolysate for adsorption and decolorization, centrifuge, take the supernatant, filter and sterilize, add butanediol and p-hydroxyacetophenone to the sterilized filtrate, mix evenly, and obtain the silk fermentation repair solution.

[0050] In step A1, the fermentation medium containing silk fibroin contains the following components by mass percentage: 3% silk fibroin, 0.4% lactose, 0.08% KH₂PO₄, 0.07% MgSO₄·7H₂O, with the remainder being deionized water; the preservation number of the *Saccharomyces cerevisiae* is GDMCC NO.2.121, and the preservation number of *Rhodotorula glutinis* is GDMCC NO.2.224; the total inoculation amount of the *Saccharomyces cerevisiae* and *Rhodotorula glutinis* is 2% v / v of the fermentation medium volume; and the total viable count of the *Saccharomyces cerevisiae* and *Rhodotorula glutinis* is 1.0 × 10⁻⁶. 9 The CFU / mL concentration of the yeast and the ratio of live bacteria to Rhizopus cylindrica were 2:1; the fermentation temperature was 28℃ and the fermentation time was 42h.

[0051] In step A2, the amount of papain added is 2.5 wt% of the fermentation product mass, the enzymatic hydrolysis temperature is 55℃ and the time is 40 min, and the enzyme inactivation temperature is 90℃ and the time is 20 min.

[0052] In step A3, based on the mass of the sterilized filtrate, the amount of butanediol added is 12.5% ​​and the amount of p-hydroxyacetophenone added is 0.4%.

[0053] Silk Fermented Repair Solution ②

[0054] Its preparation method includes the following steps:

[0055] A1. Inoculate Saccharomyces cerevisiae and Rhodotorula buergerianum into a sterilized fermentation medium containing silk fibroin for fermentation to obtain fermentation products;

[0056] A2. Add papain to the fermentation product for enzymatic hydrolysis. After complete hydrolysis, heat to inactivate the enzyme and obtain the hydrolysate.

[0057] A3. After adding activated carbon to the enzymatic hydrolysate for adsorption and decolorization, centrifuge, take the supernatant, filter and sterilize, add butanediol and p-hydroxyacetophenone to the sterilized filtrate, mix evenly, and obtain the silk fermentation repair solution.

[0058] In step A1, the fermentation medium containing silk fibroin contains the following components by mass percentage: 2% silk fibroin, 0.5% lactose, 0.1% KH₂PO₄, 0.1% MgSO₄·7H₂O, with the remainder being deionized water. The preservation number of the *Saccharomyces cerevisiae* is GDMCC NO.2.121, and the preservation number of *Rhodotorula glutinis* is GDMCC NO.2.224. The total inoculum size of the *Saccharomyces cerevisiae* and *Rhodotorula glutinis* is 1% v / v of the fermentation medium volume, and the total viable count of the *Saccharomyces cerevisiae* and *Rhodotorula glutinis* is 3.0 × 10⁻⁶. 9 The CFU / mL concentration of the yeast and the live cell ratio of Rhodotorula glutinis were 2.2:1; the fermentation temperature was 25℃ and the fermentation time was 48h.

[0059] In step A2, the amount of papain added is 3 wt% of the fermentation product mass, the enzymatic hydrolysis temperature is 50℃ and the time is 45 min, and the enzyme inactivation temperature is 85℃ and the time is 25 min.

[0060] In step A3, based on the mass of the sterilized filtrate, the amount of butanediol added is 10% and the amount of p-hydroxyacetophenone added is 0.3%.

[0061] Silk Fermented Repair Liquid ③

[0062] Its preparation method includes the following steps:

[0063] A1. Inoculate Saccharomyces cerevisiae and Rhodotorula buergerianum into a sterilized fermentation medium containing silk fibroin for fermentation to obtain fermentation products;

[0064] A2. Add papain to the fermentation product for enzymatic hydrolysis. After complete hydrolysis, heat to inactivate the enzyme and obtain the hydrolysate.

[0065] A3. After adding activated carbon to the enzymatic hydrolysate for adsorption and decolorization, centrifuge, take the supernatant, filter and sterilize, add butanediol and p-hydroxyacetophenone to the sterilized filtrate, mix evenly, and obtain the silk fermentation repair solution.

[0066] The fermentation medium containing silk fibroin contains the following components by mass percentage: 4% silk fibroin, 0.2% lactose, 0.05% KH₂PO₄, 0.05% MgSO₄·7H₂O, with the balance being deionized water. The preservation number of the *Saccharomyces cerevisiae* is GDMCC NO.2.121, and the preservation number of *Rhodotorula polleroides* is GDMCC NO.2.224. The total inoculum size of the *Saccharomyces cerevisiae* and *Rhodotorula polleroides* is 3% v / v of the fermentation medium volume, and the total viable count of the *Saccharomyces cerevisiae* and *Rhodotorula polleroides* is 7.0 × 10⁻⁶. 8 The CFU / mL concentration of the *Saccharomyces cerevisiae* and *Rhodotorula buergerianum* was 1.7:1; the fermentation temperature in step A1 was 30°C and the fermentation time was 36 h.

[0067] In step A2, the amount of papain added is 2 wt% of the fermentation product mass, the enzymatic hydrolysis temperature is 60℃ and the time is 30 min, and the enzyme inactivation temperature is 95℃ and the time is 15 min.

[0068] In step A3, based on the mass of the sterilized filtrate, the amount of butanediol added is 15% and the amount of p-hydroxyacetophenone added is 0.5%.

[0069] Example 1

[0070] A composition for long-lasting hair repair containing silk protein peptides, the composition comprising the following components by weight: 7 parts silk fermentation repair liquid ①, 6 parts silk peptides, and 14 parts silk protein surfactants;

[0071] The silk fibroin peptide, by mass percentage, comprises the following components: 7% hydrolyzed silk, 0.5% phenoxyethanol, and the balance being deionized water;

[0072] The surfactant for the silk protein is lauroyl hydrolyzed sodium silk.

[0073] The preparation method of the long-lasting hair repair composition containing silk protein peptides includes the following steps: weighing each component according to the formula amount, mixing them evenly, and obtaining each composition.

[0074] Example 2

[0075] Compared with Example 1, the difference is that this example uses silkworm fermentation repair liquid ②, while the other components, preparation steps and parameters are the same as in Example 1.

[0076] Example 3

[0077] Compared with Example 1, the difference is that this example uses silkworm fermentation repair liquid ③, while the other components, preparation steps and parameters are the same as in Example 1.

[0078] Example 4

[0079] A composition for long-lasting hair repair containing silk protein peptides, the composition comprising the following components by weight: 5 parts silk fermentation repair liquid ①, 4 parts silk peptides, and 12 parts silk protein surfactants;

[0080] The silk fibroin peptide, by mass percentage, comprises the following components: 4% hydrolyzed silk, 0.2% phenoxyethanol, and the balance being deionized water;

[0081] The surfactant for the silk protein is lauroyl hydrolyzed sodium silk.

[0082] Example 5

[0083] A composition for long-lasting hair repair containing silk protein peptides, the composition comprising the following components by weight: 10 parts of silk fermentation repair liquid ①, 9 parts of silk peptides, and 17 parts of silk protein surfactant.

[0084] The silk fibroin peptide comprises, by mass percentage, the following components: 10% hydrolyzed silk, 0.8% phenoxyethanol, and the balance being deionized water;

[0085] The surfactant for the silk protein is lauroyl hydrolyzed sodium silk.

[0086] Comparative Example 1

[0087] Compared with Example 1, the difference is that no silk fermentation repair liquid ① was added in Comparative Example 1, and the missing amount was made up with silk fibroin peptide and lauroyl hydrolyzed silk sodium in a mass ratio of 3:7. All other steps and parameters were the same as in Example 1.

[0088] Comparative Example 2

[0089] Compared with Example 1, the difference is that no silk fibroin peptides were added in Comparative Example 2, and the missing amount was made up with silk fermentation repair liquid ① and lauroyl hydrolyzed silk sodium at a mass ratio of 1:2. All other steps and parameters were the same as in Example 1.

[0090] Comparative Example 3

[0091] Compared with Example 1, the difference is that lauroyl hydrolyzed silk sodium was not added in Comparative Example 3, and the missing amount was made up with silk fermentation repair liquid ① and silk fibroin peptide in a mass ratio of 7:6. All other steps and parameters are the same as in Example 1.

[0092] Comparative Example 4

[0093] Compared with Example 1, the difference is that in Comparative Example 4, no brewer's yeast was added to the preparation of the silkworm fermentation repair solution; the inoculation amount of *Rhodotorula glutinis* was 2% v / v of the fermentation medium volume; and the viable count of *Rhodotorula glutinis* was 1.0 × 10⁻⁶. 9 The CFU / mL ratio and other steps and parameters were the same as in Example 1.

[0094] Comparative Example 5

[0095] Compared with Example 1, the difference is that the silkworm fermentation repair solution in Comparative Example 5 did not contain Rhodotorula glutinis, the inoculation amount of the brewer's yeast was 2% v / v of the fermentation medium volume, and the viable count of the brewer's yeast was 1.0 × 10⁻⁶. 9 The CFU / mL ratio and other steps and parameters were the same as in Example 1.

[0096] Comparative Example 6

[0097] Compared with Example 1, the difference is that in the preparation of the silk fermentation repair liquid in Comparative Example 6, the ratio of live bacteria of Saccharomyces cerevisiae and Rhodotorula rubra was 1.5:1, while the other steps and parameters were the same as in Example 1.

[0098] Comparative Example 7

[0099] Compared with Example 1, the difference is that in the preparation of the silk fermentation repair liquid in Comparative Example 7, the ratio of live bacteria of Saccharomyces cerevisiae and Rhodotorula buergerianum is 2.5:1, while the other steps and parameters are the same as in Example 1.

[0100] Comparative Example 8

[0101] Compared with Example 1, the difference is that the brewing yeast with accession number GDMCC NO.2.121 in Comparative Example 8 was replaced with brewing yeast with accession number CGMCC NO.2.139. All other steps and parameters are the same as in Example 1.

[0102] Comparative Example 9

[0103] Compared with Example 1, the difference is that the Rhodotorula cyclophosphamide with accession number GDMCC NO.2.224 in Comparative Example 9 was replaced with Lactobacillus paracasei with accession number CGMCC No.26528. All other steps and parameters were the same as in Example 1.

[0104] Comparative Example 10

[0105] Compared with Example 1, the difference is that the Saccharomyces cerevisiae with accession number GDMCC NO.2.121 in Comparative Example 10 was replaced with Lactobacillus paracasei with accession number CGMCC No.26528. All other steps and parameters are the same as in Example 1.

[0106] Test Example 1: Performance Testing of the Composition

[0107] Combability reflects the condition of the hair cuticle. Damaged hair has raised cuticles and a rough surface, which means it has a high combing force. In contrast, the cuticles of effectively repaired hair lie flat and have a low combing force, indicating that the hair is smooth. Therefore, combability is a direct indicator of hair smoothness and manageability, and a key parameter for measuring the immediate and long-lasting conditioning performance of hair care products.

[0108] In the dry environment of autumn and winter, static electricity causes hair to become frizzy and difficult to style. Antistatic properties are measured by static voltage, which is related to the degree of frizz and flyaway of hair in a dry environment. This reflects the product's ability to improve the balance of surface charge and smoothness of hair, and directly evaluates the moisturizing and film-forming abilities of hair care ingredients.

[0109] Tensile strength refers to the maximum stress that hair can withstand when it is pulled apart. Damaged hair loses keratin, which reduces its tensile strength and makes it more prone to breakage. The tensile strength test measures the load value that a single hair strand can withstand before it breaks, characterizing the mechanical integrity of the hair fiber and directly reflecting the repair and strengthening effect of hair care ingredients on the hair core structure.

[0110] Forty-five bundles of human hair of similar damage, bleached with hydrogen peroxide (requirements: uniform thickness, no obvious difference on the surface, and a total weight difference of ±0.01g per bundle), were prepared and randomly divided into 15 groups of three bundles each. Before testing, the hair bundles were cleaned with a 3% SDS aqueous solution and air-dried at room temperature. The long-lasting hair repair compositions prepared in Examples 1-5 and Comparative Examples 1-10 were evenly sprayed onto the hair bundles at a rate of 0.1g / g of hair. The hair bundles were rubbed for 1 minute, combed, and the compositions were evenly adhered to the hair bundles. After 10 minutes, the hair bundles were rinsed with 42°C warm water. The hair bundles were then placed on a miniature tensile testing instrument to measure the resistance encountered during combing, thus determining the wet combing performance. After the wet combing performance test, the hair bundles were placed in a constant temperature room at 25°C and 35% humidity for natural drying. After drying, the hair strands were placed on a miniature tensile tester to test their combing performance. Three parallel tests were performed, and the data are presented as the average value. The lower the value, the better the combing performance. After the combing performance test, the potential of the hair strands was measured using a vibration capacitance potentiometer. The lower the value, the better the antistatic performance. For one week, the experimental groups were uniformly sprayed with the compositions corresponding to Examples 1-5 and Comparative Examples 1-10 for long-lasting hair repair. The hair strands were placed on a multifunctional hair testing system (MTT175, Diastron, UK) for tensile testing at a tensile rate of 2 mm / min, and the tensile strength at break was recorded. The experimental results are shown in Table 1.

[0111] Compared with Example 1, Comparative Examples 1-3 did not add silk fermentation repair liquid, silk fibroin peptide, or silk protein surfactant, respectively; Comparative Examples 4-5 did not inoculate Saccharomyces cerevisiae or Rhodotorula cylindrica in the preparation process of silk fermentation repair liquid; Comparative Examples 6-7 had a ratio of viable Saccharomyces cerevisiae to Rhodotorula cylindrica in the preparation process of silk fermentation repair liquid that exceeded the scope of this application; Comparative Example 8 used Saccharomyces cerevisiae with different preservation numbers in the preparation process of silk fermentation repair liquid; and Comparative Examples 9-10 used Lactobacillus paracasei to replace Rhodotorula cylindrica and Saccharomyces cerevisiae in the preparation process of silk fermentation repair liquid, respectively.

[0112] As shown in Table 1, the combing performance, antistatic properties, and tensile strength data of Example 1 and Comparative Examples 1-3 reveal that the composition prepared from the three components—silk fermentation repair liquid, silk fibroin peptides, and silk protein surfactant—significantly improves combing performance, antistatic properties, and tensile strength. This indicates a synergistic effect among the three components. This may be because the silk protein surfactant forms a lubricating hydrophobic film on the hair surface, significantly reducing frictional resistance during wet and dry combing and effectively neutralizing static charges. The silk fermentation repair liquid, rich in small molecule peptides, amino acids, and polysaccharides, can fill the gaps between hair cuticles and smooth the hair surface. Its moisturizing and antioxidant effects can maintain the stability of the hair microenvironment, reduce static electricity accumulation, and prevent hair structural damage. Silk fibroin peptides can replenish structural proteins lost due to damage and repair the keratin network, thereby enhancing the mechanical strength of the hair. From surface lubrication and static inhibition to internal structural strengthening, the three components work together to significantly improve combing performance, antistatic properties, and tensile strength.

[0113] According to the data from Example 1 and Comparative Examples 4-10 in Table 1, the composition of the fermentation strains, the ratio of viable bacteria, and different strains all affect the fermentation effect, thereby affecting the composition's effect on improving combing performance, antistatic properties, and tensile strength. This indicates that the Saccharomyces cerevisiae and Rhodotorula glutinis selected in this application are the optimal combination of strains with synergistic effects obtained by the inventors through previous experimental screening. This may be because Saccharomyces cerevisiae and Rhodotorula glutinis promote each other during the fermentation process, which helps to hydrolyze silk fibroin into small molecule active substances, improves the permeability of active substances, and thus enhances the composition's effect on improving combing performance, antistatic properties, and tensile strength.

[0114] Table 1 Performance test data of the composition

[0115] Group Wet carding property (N) Dry carding property (N) Antistatic property (esu) Tensile strength (N) Example 1 2.1 1.3 1.7 14 2.5 Example 2 2.7 1.9 2.2 13 7.0 Example 3 2.6 1.7 2.1 13 5.4 Example 4 2.3 1.4 1.9 13 8.7 Example 5 2.2 1.5 1.8 14 0.3 Comparative Example 1 5.5 3.7 3.9 9 4.1 Comparative Example 2 5.8 4.04 0.396.4 Comparative Example 35.2 4.6 4.5 99.7 Comparative Example 44.7 3.3 3.6 110.2 Comparative Example 54.4 3.1 3.4 112.8 Comparative Example 63.9 2.8 3.0 124.6 Comparative Example 73.6 2.6 2.9 127.3 Comparative Example 84.0 2.9 3.2 118.9 Comparative Example 94.3 3.0 3.1 120.5 Comparative Example 104.4 3.2 3.2 116.0 surface

[0116] Application example: A conditioner with long-lasting hair repair effects.

[0117] The compositions of Examples 1-5 and Comparative Examples 1-10 were added to hair conditioners to obtain hair conditioners of Application Examples 1-5 and Comparative Application Examples 1-10, respectively.

[0118] The hair conditioner with long-lasting hair repair effect comprises the following ingredients by weight percentage: 1.5% of the long-lasting hair repair composition, 7% sodium hyaluronate, 6% hydroxypropyl methylcellulose, 4% glyceryl stearate, 1% behenamidopropyl dimethylamine, 1% potassium sorbate, and the balance being deionized water.

[0119] The preparation method of the conditioner with long-lasting hair repair effect specifically includes the following steps:

[0120] S1. Sodium hyaluronate, hydroxypropyl methylcellulose, glyceryl stearate and deionized water are mixed and stirred at 200 rpm to obtain a mixture.

[0121] S2. Add behenamidopropyl dimethylamine, potassium sorbate and each component of the long-lasting hair repair composition to the mixture, and stir and mix at 300 rpm to obtain the hair conditioner.

[0122] Compare with application example 11 (blank application example)

[0123] Compared with Application Example 1, the difference is that the conditioner in Application Example 11 does not contain a long-lasting hair repair composition, and uses an equal amount of deionized water instead of the composition. The preparation method is the same as in Application Example 1.

[0124] Test Example 2: Irritation Resistance, Smoothing Effect, and Gloss Effect Test

[0125] Three hundred and twenty volunteers aged 23-60 with similar degrees of hair damage were selected and divided into 16 groups of 20 each, with an equal number of men and women. The test samples used in Examples 1-5 and Control Examples 1-11 were applied. Each participant applied a small amount of the test sample behind their ear and left it for 20 minutes to observe for any discomfort, redness, or rash. The results showed that no adverse symptoms appeared in any participant, allowing for further testing. The sample was used twice a day, 5g each time, for three consecutive months. During the testing period, the sample was not used. He compared this product with similar products, evaluating its anti-irritant properties, smoothing effect, and shine effect; and used shampoo to clean the hair for two days prior to the evaluation, and did not use any products afterward; after scoring, the average score for each group was calculated (rounded to one decimal place). Evaluation criteria: Anti-irritant properties: 9.1-10.0 points: No skin discomfort, erythema, itching, stinging, peeling, etc. during or after use; 8.1-9.0 points: Occasionally slight tightness, but no visible skin reaction; 6.1-8.0 points: Occasionally slight stinging or itching, lasting less than 5 minutes. 3.1-6.0 points: Transient erythema or mild itching occurs, subsiding spontaneously within 24 hours; 0.0-3.0 points: Obvious redness, papules, persistent itching, or stinging occurs, product should be discontinued; Smoothing effect: 9.1-10.0 points: Completely no resistance, hair is silky smooth, without tangles or frizz; 8.1-9.0 points: Slight resistance, mostly smooth, with occasional slight frizz at the ends; 6.1-8.0 points: Medium resistance, requires slightly more effort to comb, with localized tangles or frizz; 3.1-6.0 points: Obvious resistance, multiple tangles. Difficult to comb smoothly; 0.0-3.0 points: Severely tangled, easily breaks when combing, extremely dry and rough; Shine effect: 9.1-10.0 points: Hair has a mirror-like shine; 8.1-9.0 points: Obvious shine, even reflection on the hair surface; 6.1-8.0 points: Medium shine, weak reflection but overall bright; 3.1-6.0 points: Weak shine, only a faint reflection is visible in some areas, overall slightly dry; 0.0-3.0 points: Almost no reflection, dull and dry hair surface, appears dry and lackluster even in bright environments;

[0126] Table 2 Experimental Data

[0127] Group | Anti-irritant effect | Smoothing effect | Application example 1 | 10.0 | 9.7 | 9.3 | Application example 2 | 10.0 | 9.0 | 8.8 | Application example 3 | 10.0 | 9.1 | 8.9 | Application example 4 | 10.0 | 9.4 | 9.1 | Application example 5 | 10.0 | 9.6 | 9.2 | Comparison application example 1 | 10.0 | 5.9 | 5.8 | Comparison application example 2 | 10.0 | 5.4 | 5.3 | Comparison application example 3 | 10.0 | 5.2 | 5.5 | Comparison application example 4 | 10.0 | 6.5 | 6.4 | Comparison application example 5 | 10.0 | 6.7 | 6.6 | Comparison application example 6 | 10.0 | 7.6 | 7.4 | Comparison application example 7 | 10.0 | 7.8 | 7.7 | Comparison application example 8 | 10.0 | 6.9 | 7.1 | Comparison application example 9 | 10.0 | 6.7 | 6.8 | Comparison application example 10 | 10.0 | 6.6 | 6.5 | Comparison application example 11 | 10.0 | 4.3 | 4.0 surface

[0128] Test Example 3: Contact Angle Test

[0129] In Test Example 2, the volunteers had their contact angles measured using a contact angle meter before using the sample and after using the sample five times. The contact angle difference was calculated using the following formula, and the average for each group was recorded, as shown in Table 3.

[0130] ;

[0131] In the formula: A0 is the contact angle before using the sample, and A5 is the contact angle after using the sample 5 times;

[0132] Table 3 Contact Angle Test Data

[0133] Group Contact Angle Difference / ° Example 1: 40.12 Example 2: 34.56 Example 3: 35.71 Example 4: 38.09 Example 5: 37.45 Comparison: Example 1: 25.93 Comparison: Example 2: 27.88 Comparison: Example 3: 24.64 Comparison: Example 4: 28.50 Comparison: Example 5: 29.16 Comparison: Example 6: 35.02 Comparison: Example 7: 36.23 Comparison: Example 8: 32.47 Comparison: Example 9: 29.31 Comparison: Example 10: 28.75 Comparison: Example 11: 1.84 surface

[0134] Compared with Application Example 1, the compositions in Comparative Application Examples 1-3 did not contain silk fermentation repair liquid, silk fibroin peptides, or silk protein surfactants, respectively. The preparation processes of Comparative Application Examples 4-5 for the silk fermentation repair liquid did not include inoculation with Saccharomyces cerevisiae or Rhodotorula glutinis. The ratio of viable Saccharomyces cerevisiae to Rhodotorula glutinis in the preparation processes of Comparative Application Examples 6-7 exceeded the scope of this application. The preparation process of Comparative Application Example 8 used Saccharomyces cerevisiae with different preservation numbers in the preparation process of the silk fermentation repair liquid. The preparation processes of Comparative Examples 9-10 used Lactobacillus paracasei to replace Rhodotorula glutinis and Saccharomyces cerevisiae, respectively.

[0135] Based on the fact that the anti-irritation scores of Application Examples 1-5 and Comparative Application Examples 1-11 in Table 2 are all 10.0, it can be seen that the hair conditioner prepared in this application has the advantage of being mild and non-irritating.

[0136] As can be seen from the difference in smoothness, shine, and contact angle between Application Example 1 and Comparative Application Examples 1-3 in Tables 2-3, the conditioner prepared with the three components of silk fermentation repair liquid, silk peptide, and silk protein surfactant has better smoothness, shine, and repair effects. On the one hand, the silk protein surfactant and silk fermentation repair liquid protect and repair the hair cuticle, and on the other hand, the silk peptide repairs the internal structure of the hair. The three components of silk fermentation repair liquid, silk peptide, and silk protein surfactant work together to achieve long-lasting smoothness, shine, and hair repair effects.

[0137] According to the data from Application Example 1 and Comparative Application Examples 4-10 in Tables 2-3, the composition of the fermentation strains, the ratio of live bacteria, and different strains all affect the fermentation effect, thereby affecting the smoothness, shine, and repair effect of the conditioner. This indicates that the Saccharomyces cerevisiae and Rhodotorula glutinis selected in this application are the optimal combination of strains with synergistic effects obtained by the inventors through previous experimental screening. This may be because Saccharomyces cerevisiae and Rhodotorula glutinis promote each other during the fermentation process, which helps to hydrolyze silk fibroin into small molecule active substances, improves the penetration rate of active substances, and thus enhances the smoothness, shine, and repair effect of the conditioner.

[0138] The above description is merely a preferred composition of the present invention and is not intended to limit the present invention in any way. Although the present invention has been disclosed above with preferred compositions, it is not intended to limit the present invention. Any person skilled in the art can make some modifications or alterations to the above-disclosed technical content to create equivalent compositions without departing from the scope of the present invention. Any simple modifications, equivalent changes and alterations made to the above compositions based on the technical essence of the present invention without departing from the scope of the present invention shall still fall within the scope of the present invention.

Claims

1. A composition containing silk protein peptides for long-lasting hair repair, characterized in that, The composition comprises, by weight, the following components: 5-10 parts of silk fermentation repair solution, 4-9 parts of silk fibroin peptide, and 12-17 parts of silk protein surfactant; the preparation method of the silk fermentation repair solution includes the following steps: A1, inoculating sterilized fermentation medium containing silk fibroin with Saccharomyces cerevisiae and Rhodotorula glutinis for fermentation to obtain fermentation products; wherein, the fermentation medium containing silk fibroin comprises, by weight percentage, the following components: 2%-4% silk fibroin, 0.2%-0.5% lactose, 0.05%-0.1% KH2PO4, 0.05%-0.1% MgSO4·7H2O, with the balance being deionized water; the preservation number of the Saccharomyces cerevisiae is GDMCC NO.2.121, and the preservation number of the Rhodotorula glutinis is GDMCC. NO.2.224; A2. Papain is added to the fermentation product for enzymatic hydrolysis. After complete hydrolysis, the enzyme is inactivated by heating to obtain the hydrolysate. The amount of papain added is 2-3 wt% of the fermentation product, the enzyme activity of the papain is 800-1000 U / mg, the hydrolysis temperature is 50-60℃ and the time is 30-45 min, and the enzyme inactivation temperature is 85-95℃ and the time is 15-25 min. A3. Activated carbon is added to the hydrolysate for adsorption and decolorization treatment. After centrifugation, the supernatant is filtered and sterilized. Butylene glycol and p-hydroxyacetophenone are added to the sterilized filtrate and mixed evenly to obtain the silk fermentation repair solution. The silk fibroin peptide contains the following components by mass percentage: 4%-10% hydrolyzed silk, 0.2%-0.8% phenoxyethanol, and the balance is deionized water. The silk fibroin surfactant is lauroyl hydrolyzed silk sodium.

2. The composition according to claim 1, characterized in that, The composition comprises the following components by weight: 7 parts silk fermentation repair liquid, 6 parts silk fibroin peptide and 14 parts silk protein surfactant.

3. The composition according to claim 1, characterized in that, In step A1, the total inoculation amount of *Saccharomyces cerevisiae* and *Rhodotorula glutinis* is 1-3% v / v of the fermentation medium volume; the total viable count of *Saccharomyces cerevisiae* and *Rhodotorula glutinis* is 7.0 × 10⁻⁶. 8 -3.0×10 9 CFU / mL.

4. The composition according to claim 3, characterized in that, In step A1, the ratio of live Saccharomyces cerevisiae to Rhodotorula glutinis is 1.7-2.2:

1.

5. The composition according to claim 3, characterized in that, In step A1, the ratio of live bacteria of Saccharomyces cerevisiae to Rhodotorula glutinis is 2:

1.

6. The composition according to claim 1, characterized in that, The fermentation temperature in step A1 is 25-30℃ and the time is 36-48h.

7. The composition according to claim 1, characterized in that, In step A3, based on the mass of the sterilized filtrate, the amount of butanediol added is 10-15%, and the amount of p-hydroxyacetophenone added is 0.3-0.5%.

8. The use of the composition according to any one of claims 1-7 in the preparation of a shampoo and conditioner product with long-lasting hair repair effects, characterized in that, The hair care products include shampoo, hair serum, hair oil, hair conditioner, hair mask, and hair treatment.

9. A hair conditioner, characterized in that, The raw materials include the following percentages by weight: 0.1%-2% of the composition according to any one of claims 1-7, 5%-10% of a humectant, 3%-8% of a thickener, 2%-6% of an emulsifier, 0.5%-1.5% of a conditioning agent, 0.1%-1.5% of a preservative, and the balance being deionized water; wherein the humectant is selected from at least one of glycerin, butylene glycol, propylene glycol, betaine, sodium hyaluronate, and panthenol; wherein the thickener is selected from at least one of hydroxypropyl methylcellulose, carbomer, xanthan gum, and cetearyl alcohol; and wherein the emulsifier is selected from glyceryl stearate and oleyl alcohol. The formula includes at least one of polyether-10, PEG-100 stearate, PEG-10 polydimethylsiloxane, cetearyl glucoside, sodium stearoyl lactylate, and sodium stearoyl glutamate; the conditioning agent is selected from at least one of behenyltrimethylammonium chloride, behenyltrimethylammonium methyl sulfate, behenamidopropyl dimethylamine, stearamidopropyl dimethylamine, stearyltrimethylammonium chloride, and stearyltrimethylammonium methyl sulfate; and the preservative is selected from at least one of phenoxyethanol, sodium benzoate, potassium sorbate, and p-hydroxyacetophenone.

10. A method for preparing the hair conditioner as described in claim 9, characterized in that, Includes the following steps: S1. Mix the humectant, thickener, emulsifier and deionized water, and stir at 150-250 rpm to obtain a mixture; S2. Add the conditioner, preservative and the long-lasting hair repair composition as described in any one of claims 1-7 to the mixture, and stir at 250-350 rpm to obtain the hair conditioner.