Cosmetic preservative composition and application thereof

Abstract

The invention relates to a cosmetic preservative composition and application thereof. The cosmetic preservative composition comprises a lactic acid bacteria fermentation product, ethylhexylglycerin, polyhydric alcohol, bisabolol, lactobacillus / soybean milk fermentation product filtrate and kava pepper leaf / root / stem extract. The cosmetic preservative composition provided by the invention can provide strong, broad-spectrum, lasting and stable preservative activity, also has a good anti-allergy soothing effect, and can maintain natural properties, good sense and batch consistency at the same time.

Description

Technical Field

[0001] This invention relates to the field of cosmetic technology, and more particularly to a cosmetic preservative composition and its application. Background Technology

[0002] Cosmetics are rich in various nutrients, making them highly susceptible to microbial growth during production, storage, and use. Once contaminated, products will exhibit signs of spoilage such as turbidity, sedimentation, discoloration, and altered odor. If contaminated with pathogenic bacteria (such as Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Aspergillus niger, Candida albicans, and yeast), these pathogens and their metabolic products may also harm human health. Therefore, to ensure the safety and hygiene of cosmetics, appropriate preservatives must be added to inhibit microbial growth.

[0003] An ideal preservative should have the following characteristics: it can effectively inhibit the growth of microorganisms; it is non-toxic, harmless, and non-irritating to the human body; it is chemically stable and does not easily react with other ingredients in cosmetics, thus maintaining a long-lasting and stable preservative effect.

[0004] Currently, the types of synthetic preservatives permitted for use in cosmetics and their maximum permitted amounts are clearly stipulated by the national regulation, "Cosmetic Hygiene Standards." However, due to the potential safety hazards and biotoxicity of these synthetic preservatives, their usage is strictly limited. In recent years, skin problems caused by the use of preservatives in cosmetics have been increasing, leading to numerous adjustments to cosmetic preservative regulations. These include banning certain parabens, lowering the limit for iodopropynylcarbamate, and restricting the use of methylisothiazolinone. Simultaneously, consumers have gradually recognized the harm caused by synthetic preservatives and have begun to seek preservative-free cosmetics. Natural preservatives are gentler and less allergenic, aligning with the concept of pure skincare and meeting consumers' dual pursuit of safety and naturalness, thus driving market demand for alternatives to traditional synthetic preservatives.

[0005] Therefore, developing novel solutions that can provide potent, broad-spectrum, and long-lasting stable preservative activity in conventional cosmetic systems while maintaining natural properties, good sensory qualities, and batch-to-batch consistency is a core technological challenge that urgently needs to be overcome in the field of natural cosmetics. Summary of the Invention

[0006] To address the aforementioned technical problems, this invention provides a cosmetic preservative composition and its application. The cosmetic preservative composition provided by this invention offers potent, broad-spectrum, and long-lasting stable preservative activity, while also exhibiting good anti-sensitivity and soothing effects, and maintaining natural properties, good sensory qualities, and batch-to-batch consistency.

[0007] To achieve this objective, the present invention adopts the following technical solution:

[0008] In a first aspect, the present invention provides a cosmetic preservative composition comprising lactic acid bacteria fermentation product, ethylhexylglycerin, polyol, bisabolol, lactobacillus / soybean milk fermentation product filtrate, and kava leaf / root / stem extract.

[0009] In this invention, the lactic acid bacteria fermentation product (antimicrobial peptide) carries a positive charge. Under a certain membrane potential, it can be adsorbed onto the cell membrane of pathogenic bacteria, accumulate on the cell membrane surface, and insert into the cell membrane through the C-terminus to form a permeable pore. The cell membrane loses polarization, resulting in the loss of intracellular small molecule solutes (ATP, amino acids, ions, etc.) and the inflow of extracellular water molecules, causing the energy difference between the inside and outside of the cell membrane to disappear, and the cell to autolyze and die.

[0010] In this invention, ethylhexylglycerol can penetrate and disrupt the cell membrane of microorganisms, reducing their barrier function; it helps to destroy or prevent biofilm formation, making microorganisms easier to eliminate.

[0011] In this invention, polyols can penetrate the cell membrane of microorganisms, thereby disrupting the permeability of the cell membrane and causing cell death.

[0012] In this invention, bisabolol is a natural active ingredient extracted from plants such as chamomile and geranium, which has anti-inflammatory, soothing, and repairing effects, and can induce cell cycle arrest and mitochondrial death.

[0013] In this invention, the lactobacillus / soy milk fermentation product filtrate can inhibit the excessive proliferation of harmful bacteria (such as Staphylococcus aureus) and can also improve skin health by regulating the skin microecology, enhancing barrier function, and soothing inflammation.

[0014] In this invention, the extract of kava leaves / roots / stems has a certain inhibitory effect on Propionibacterium acnes and can also soothe and reduce inflammation.

[0015] In this invention, the synergistic anti-corrosion mechanism of the six core substances is as follows: First, polyols such as butanediol, 1,2-pentanediol, and 1,2-hexanediol rapidly adsorb environmental moisture, reducing water activity and inhibiting the basic reproductive capacity of microorganisms; subsequently, these polyols, in order of increasing hydrophobicity, insert their hydrophobic ends into the phospholipid layer of the microbial cell membrane, loosening the membrane structure and causing the outflow of potassium and calcium ions; then, ethylhexylglycerol penetrates and destroys the biofilm matrix formed by the microbial community, disintegrating extracellular polymers, exposing individual cells, and simultaneously reducing... Membrane surface tension, in synergy with polyols, thoroughly disrupts membrane integrity, forming permanent pores. Then, small-molecule organic acids (such as lactic acid) from Lactobacillus fermentation products and Lactobacillus / soybean milk fermentation filtrate enter the cells through these pores, dissociating intracellularly to release hydrogen ions, lowering intracellular pH, and disrupting enzyme activity and proton gradients. Simultaneously, bisabolol interferes with microbial metabolism, ultimately leading to the loss of key cofactors such as intracellular magnesium ions, resulting in microbial metabolic paralysis. Kava pepper leaf / root / stem extracts provide multi-target supplementation and inhibition of residual fungi. At the same time, the system simultaneously initiates a repair mechanism: polyols and ethylhexylglycerin work together to maintain stratum corneum hydration; the prebiotics and acidic environment provided by Lactobacillus fermentation products help stabilize the skin microecology and strengthen the barrier; and bisabolol directly soothes the skin and reduces inflammation, thus achieving active skin repair during highly effective preservation.

[0016] This invention selects a potent, broad-spectrum, long-lasting, stable, non-reactive, and synergistic combination of preservatives, which also has good anti-allergic and soothing effects. It can not only provide potent, broad-spectrum, and long-lasting stable preservative activity, but also maintain natural properties, good sensory properties, and batch consistency.

[0017] Preferably, the cosmetic preservative composition comprises, by weight parts, 0.1-5 parts of lactic acid bacteria fermentation product (e.g., 0.1, 0.3, 0.5, 2, 3, 5, etc.), 0.05-1 part of ethylhexylglycerin (e.g., 0.05, 0.1, 0.3, 0.6, 1, etc.), 0.5-20 parts of polyol (e.g., 0.5, 1, 3, 7, 11, 16, 20, etc.), 0.015-0.03 parts of bisabolol (e.g., 0.015, 0.02, 0.025, 0.03, etc.), 1-5 parts of lactic acid bacteria / soybean milk fermentation product filtrate (e.g., 1, 2, 3, 4, 5, etc.), and 1-5 parts of kava leaf / root / stem extract (e.g., 1, 2, 3, 4, 5, etc.).

[0018] Preferably, the polyol is a C4-C10 polyol (e.g., it can be C4, C5, C6, C8, C10, etc.).

[0019] Preferably, the polyol includes any one or a combination of at least two of butanediol, 1,2-hexanediol, or 1,2-pentanediol.

[0020] Preferably, the polyol comprises a combination of butanediol, 1,2-hexanediol and 1,2-pentanediol.

[0021] In this invention, butanediol is a small-molecule polyol that can penetrate the cell membrane of microorganisms, thereby disrupting the permeability of the cell membrane and interfering with its integrity. It can also cause an imbalance in osmotic pressure inside and outside the cell, allowing a large amount of water to enter the cell, leading to cell swelling and even rupture (bacteriolytic effect).

[0022] The 6-carbon chain structure of 1,2-hexanediol is highly hydrophobic, allowing it to penetrate the phospholipid bilayer of microbial (bacterial, fungal) cell membranes and disrupt the membrane's orderly arrangement. The two hydroxyl groups (-OH) bind to water molecules on the cell membrane surface via hydrogen bonds, leading to localized dehydration and embrittlement. The combined effect of the hydrophobic chain and hydrophilic group dehydration creates pores in the cell membrane, ultimately triggering cell lysis and death.

[0023] The five-carbon chain (C5) of 1,2-pentanediol is highly hydrophobic, allowing it to insert into the phospholipid layer of microbial cell membranes, disrupting the membrane's orderly arrangement and increasing permeability. The two hydroxyl groups (-OH) bind to water molecules on the cell membrane surface, causing localized dehydration and weakening membrane stability. Damage to the membrane structure leads to the leakage of small intracellular molecules, causing osmotic pressure imbalance and ultimately cell death. It can interfere with the activity of key microbial enzymes (such as dehydrogenases and ATPases), blocking energy metabolism pathways, with a more pronounced effect on fungi (such as Candida albicans).

[0024] In this invention, the anti-corrosion effect is further improved when a compound combination of butanediol, 1,2-hexanediol and 1,2-pentanediol is used.

[0025] Preferably, the mass ratio of butanediol, 1,2-hexanediol and 1,2-pentanediol is (1-10):(0.2-5):1.

[0026] The values ​​(1-10) can be, for example, 1, 2, 5, 8, 10, etc., and (0.2-5) can be, for example, 0.2, 1, 2, 3, 5, etc.

[0027] In a second aspect, the present invention provides the application of the cosmetic preservative composition according to the first aspect in cosmetics.

[0028] Preferably, the cosmetic includes creams, serums, masks, lotions, or emulsions.

[0029] Thirdly, the present invention provides a cream comprising a preservative, an antioxidant, a moisturizer, an emulsifier, a skin moisturizer, a thickener, a pH neutralizer, a repairing agent, and water, wherein the preservative comprises the cosmetic preservative composition described in the first aspect.

[0030] Preferably, the cream comprises, by weight percentage, 0.3-25% preservatives (e.g., 0.3%, 1.6%, 3%, 5%, 10%, 15%, 20%, etc.), 0.1-10% antioxidants (e.g., 0.1%, 1%, 2%, 5%, 10%, etc.), 1-15% moisturizers (e.g., 1%, 2%, 5%, 10%, 15%, etc.), 1-10% emulsifiers (e.g., 1%, 2%, 5%, 10%, etc.), and 3-30% skin moisturizers. (e.g., 3%, 5%, 10%, 20%, 30%, etc.), thickener 0.1-2% (e.g., 0.1%, 0.5%, 1%, 2%, etc.), pH neutralizer 0.01-1% (e.g., 0.01%, 0.05%, 0.5%, 1%, etc.), repair agent 0.1-10% (e.g., 0.1%, 1%, 2%, 5%, 10%, etc.), and water 50-70% (e.g., 50%, 55%, 60%, 65%, 70%, etc.).

[0031] Preferably, the antioxidant comprises any one or a combination of at least two of ubiquinone, tocopheryl acetate, or carnosine.

[0032] Preferably, the humectant comprises any one or a combination of at least two of glycerol polyether-26, allantoin, betaine, biosaccharide gum-1, or sugar isomers.

[0033] Preferably, the emulsifier comprises any one or a combination of at least two of oil palm oil, oil palm kernel oil, behenol, polyglycerol-10 pentastearate, or sodium stearoyl lactylate.

[0034] Preferably, the emollient comprises any one or a combination of at least two of isononyl isononanoate, shea butter, triglyceride (ethylhexanoate), squalane, or jojoba seed oil.

[0035] Preferably, the thickener comprises any one or a combination of at least two of the following: sodium polyacrylamide dimethyl taurate, acrylate / C10-30 alkanol acrylate crosspolymer, or ammonium acrylamide dimethyl taurate / VP copolymer.

[0036] Preferably, the pH neutralizing agent comprises any one or a combination of two of aminopropanol or triethanolamine.

[0037] Preferably, the repairing agent comprises any one or a combination of at least two of the following: soft-haired pine algae extract, tetrahydromethylpyrimidine carboxylic acid, wheat flour lipids, or fibronectin.

[0038] Fourthly, the present invention provides a method for preparing the cream according to the third aspect, the method comprising the following steps:

[0039] (1) Mix water, thickener and preservative polyol to obtain an aqueous phase; mix emollient and emulsifier to obtain an oil phase;

[0040] (2) Mix and homogenize the aqueous phase and the oil phase, and then mix with the remaining components in the pH neutralizer, antioxidant, moisturizer, repair agent and preservative to obtain the cream.

[0041] Compared with the prior art, the present invention has at least the following beneficial effects:

[0042] The cosmetic preservative composition provided by this invention has a synergistic effect among the raw materials, namely lactic acid bacteria fermentation product, ethylhexylglycerin, polyol, bisabolol, lactobacillus / soy milk fermentation product filtrate and kava leaf / root / stem extract. It can provide strong, broad-spectrum and long-lasting stable preservative activity, and also has good anti-sensitivity and soothing effects, while maintaining natural properties, good sensory properties and batch consistency. Detailed Implementation

[0043] To facilitate understanding of the present invention, the following embodiments are provided. Those skilled in the art should understand that these embodiments are merely illustrative and should not be construed as limiting the scope of the invention.

[0044] The materials used in the following embodiments and application examples are sourced from the following sources:

[0045] Lactic acid bacteria fermentation products, manufactured by Haoer Biotechnology; Lactic acid bacteria / soy milk fermentation product filtrate, manufactured by Xianting (Guangzhou) Trading Co., Ltd.; Kava pepper (PIPER METHYSTICUM) leaf / root / stem extract, manufactured by AXIALYSINNOVATIONS.

[0046] The weight percentages of the raw materials mentioned above are based on the actual content of active ingredients in commercially available raw materials. Commercially available raw materials may also selectively contain any one or a combination of at least two of the following: solvents, fillers, diluents, stabilizers, pH adjusters, antibacterial agents, antioxidants, or impurities within permissible limits.

[0047] HAMIN-C (oil palm (ELAEIS GUINEENSIS) oil, oil palm (ELAEIS GUINEENSIS) kernel oil), manufacturer: oleo pharma; CODIUM TOMENTOSUM extract, manufacturer: Codif International; Wheat (TRITICUM VULGARE) fine powder lipids, manufacturer: ENNAGRAM; NIKKOMULESE 41 (behenol, polyglycerol-10 pentastearate, sodium stearoyl lactylate), manufacturer: NIPPON SURFACTANT INDUSTRIES CO.,LTD.

[0048] Example 1

[0049] This embodiment provides a cosmetic preservative composition comprising, by weight parts, 2 parts of lactic acid bacteria fermentation product, 0.08 parts of ethylhexylglycerin, 10 parts of polyol (butanediol, 1,2-hexanediol and 1,2-pentanediol in a mass ratio of 5:2:1), 0.02 parts of bisabolol, 2.5 parts of lactic acid bacteria / soybean milk fermentation product filtrate, and 2.5 parts of kava leaf / root / stem extract.

[0050] Example 2

[0051] This embodiment provides a cosmetic preservative composition comprising, by weight parts, 0.1 parts of lactic acid bacteria fermentation product, 1 part of ethylhexylglycerin, 0.5 parts of polyol (butanediol, 1,2-hexanediol and 1,2-pentanediol in a mass ratio of 1:5:1), 0.03 parts of bisabolol, 1 part of lactic acid bacteria / soy milk fermentation product filtrate, and 5 parts of kava leaf / root / stem extract.

[0052] Example 3

[0053] This embodiment provides a cosmetic preservative composition comprising, by weight parts, 5 parts of lactic acid bacteria fermentation product, 0.05 parts of ethylhexylglycerin, 20 parts of polyol (butanediol, 1,2-hexanediol and 1,2-pentanediol in a mass ratio of 10:0.2:1), 0.015 parts of bisabolol, 5 parts of lactic acid bacteria / soy milk fermentation product filtrate, and 1 part of kava leaf / root / stem extract.

[0054] Example 4

[0055] This embodiment provides a cosmetic preservative composition, which differs from Example 1 only in that the polyol is butanediol and 1,2-hexanediol in a mass ratio of 5:2, and the total amount of polyol remains unchanged. Other aspects are the same as in Example 1.

[0056] Example 5

[0057] This embodiment provides a cosmetic preservative composition, which differs from Example 1 only in that the polyol is butanediol and 1,2-pentanediol in a mass ratio of 5:1, and the total amount of polyol remains unchanged. Other aspects are the same as in Example 1.

[0058] Example 6

[0059] This embodiment provides a cosmetic preservative composition, which differs from Example 1 only in that the polyols are 1,2-hexanediol and 1,2-pentanediol in a mass ratio of 2:1, the total amount of polyols remains unchanged, and other aspects are the same as in Example 1.

[0060] Comparative Example 1

[0061] This comparative example provides a cosmetic preservative composition, which differs from Example 1 only in that it lacks lactic acid bacteria fermentation product, and its amount is allocated according to the proportion of the remaining components. Other aspects are the same as in Example 1.

[0062] Comparative Example 2

[0063] This comparative example provides a cosmetic preservative composition, which differs from Example 1 only in that it lacks ethylhexylglycerin, and its amount is allocated according to the proportion of the remaining components; otherwise, it is the same as in Example 1.

[0064] Comparative Example 3

[0065] This comparative example provides a cosmetic preservative composition, which differs from Example 1 only in that it lacks a polyol, and its amount is allocated according to the proportion of the remaining components. Other aspects are the same as in Example 1.

[0066] Comparative Example 4

[0067] This comparative example provides a cosmetic preservative composition, which differs from Example 1 only in that it lacks bisabolol, and its dosage is allocated according to the proportion of the remaining components. Other aspects are the same as in Example 1.

[0068] Comparative Example 5

[0069] This comparative example provides a cosmetic preservative composition, which differs from Example 1 only in that it lacks the lactobacillus / soy milk fermentation product filtrate, and its amount is allocated according to the proportion of the remaining components. Other aspects are the same as in Example 1.

[0070] Comparative Example 6

[0071] This comparative example provides a cosmetic preservative composition, which differs from Example 1 only in that it lacks the kava leaf / root / stem extract, and its amount is allocated according to the proportion of the remaining components; otherwise, it is the same as in Example 1.

[0072] Application Example 1

[0073] This application example provides a cream, the types of its components and their amounts are shown in Table 1.

[0074] Table 1

[0075]

[0076] The preparation method of the cream includes the following steps:

[0077] (1) Water pot process: Add deionized water, then slowly add the polyol in the thickener and preservative, stir until there are no large particles, heat to 80°C, stir until completely dissolved, vacuum the material into the main pot, and keep it at 80°C while stirring slowly.

[0078] (2) Oil pot process: Add moisturizer and emulsifier, heat to 70°C, and after complete dissolution, vacuum the material into the main pot;

[0079] (3) Main pot process: Mix the water pot material and the oil pot material evenly, turn on the homogenizer, and after the homogenization is complete, start cooling down to 50°C. Add pH neutralizer, stir evenly, and then cool down to 40°C. Add the remaining components of the preservative, antioxidant, moisturizer, repair agent and essential oil, and stir evenly to obtain the cream.

[0080] Application Example 2-6

[0081] Application Examples 2-6 each provide a cream, which differs from Application Example 1 only in that the cosmetic preservative composition provided in Example 1 is replaced in equal amounts with the cosmetic preservative compositions provided in Examples 2-6, and the rest is the same as in Application Example 1.

[0082] Comparative Application Examples 1-6

[0083] Comparative Application Examples 1-6 each provide a cream, the only difference from Application Example 1 being that the cosmetic preservative composition provided in Example 1 is replaced in equal amounts with the cosmetic preservative compositions provided in Comparative Examples 1-6, and the rest is the same as in Application Example 1.

[0084] Test Example 1

[0085] Corrosion resistance challenge evaluation experiment

[0086] (1) Experimental strains

[0087] Bacteria: Staphylococcus aureus ATCC6538, Escherichia coli ATCC8739, Pseudomonas aeruginosa ATCC9027;

[0088] Fungi: Candida albicans ATCC10231, Aspergillus brasiliensis ATCC16404.

[0089] (2) Preparation of mixed bacterial culture

[0090] The bacteria to be tested were inoculated onto nutrient agar slants and incubated at 36℃±1℃ for 24 hours to obtain bacterial suspensions for each bacterium. The bacterial suspensions were then divided into 10... 5 10 6 10 7 Three dilutions were cultured on nutrient agar to confirm the viable cell count in each culture. The cultures of each bacterium were then diluted with physiological saline to a final concentration of 1.2 × 10⁻⁶. 8 The concentrations were CFU / mL, and then equal volumes were mixed to obtain a mixed bacterial culture.

[0091] (3) Preparation of mixed fungal culture

[0092] The fungi to be tested were inoculated onto Sabouraud dextrose agar slants. *Candida albicans* was incubated at 28℃±2℃ for 24 hours, and *Aspergillus brasiliensis* was incubated at 28℃±2℃ for 5 days to obtain bacterial suspensions. The bacterial suspensions were then cultured at a ratio of 10... 5 10 6 10 7 Three dilutions were cultured on Sabouraud agar to confirm the viable cell count of each culture. The cultures of each bacterium were then diluted with physiological saline to a final concentration of 2.0 × 10⁻⁶. 7 The concentrations were CFU / mL, and then equal volumes were mixed to obtain a mixed fungal culture.

[0093] (4) Addition of bacteria to the sample and detection

[0094] Under aseptic conditions, weigh 100g of the sample into two sterile reagent bottles. Add 1mL of mixed bacterial suspension and 1mL of mixed fungal suspension to each bottle. Mix the bacterial suspension and sample thoroughly and store at room temperature (20℃-25℃) away from light. Detect the bacterial colony count in the cosmetics on days 7, 14, 21, and 28 after bacterial addition.

[0095] The logarithmic decrease in colony count (R) was used. X () is used as an evaluation indicator. The calculation method is as follows:

[0096] R X =lgN O -lgN X ;

[0097] Where: N O —The initial concentration of the bacterial culture in the sample;

[0098] N X —The number of bacterial colonies in the sample at different testing times.

[0099] The creams provided in Application Examples 1-6 and Comparative Application Examples 1-6 were subjected to a preservative challenge evaluation experiment. The judgment criteria are shown in Table 2. XThe test results are summarized in Table 3.

[0100] Table 2

[0101]

[0102] Note: NI indicates no increase compared to the previous test time.

[0103] Table 3

[0104]

[0105] The test results show that:

[0106] (1) As can be seen from Application Examples 1 to 6, the present invention combines lactic acid bacteria fermentation product, ethylhexylglycerin, polyol, bisabolol, lactobacillus / soy milk fermentation product filtrate and kava leaf / root / stem extract. Each component has a synergistic effect in preservation and has a strong preservative effect.

[0107] (2) By comparing Application Example 1 and Application Examples 4-6, it can be seen that the present invention can achieve better anti-corrosion technical effect by further adopting a polyol system of butanediol, 1,2-hexanediol and 1,2-pentanediol.

[0108] (3) By comparing Application Example 1 and Comparative Application Examples 1-6, it can be seen that when any one of the following is missing from the composition: lactic acid bacteria fermentation product, ethylhexylglycerin, polyol, bisabolol, lactobacillus / soy milk fermentation product filtrate or kava pepper leaf / root / stem extract, the antibacterial effect is weakened and it cannot pass the preservation test.

[0109] Test Example 2

[0110] Thirty female subjects were selected. Each subject used the cream provided in Examples 1-6. A patch test tape containing 0.03 mL of sample was applied to the normal skin of the subject's left forearm. After the patch was removed, the subject was examined according to the CTFA guidelines (1981) within half an hour. The test was performed 6 times. On the 6th day after the patch was applied, an additional test was performed to observe delayed reactions. The identification criteria are shown in Table 4, and the results are shown in Table 5.

[0111] Table 4

[0112]

[0113] Table 5

[0114]

[0115] The above data results show that the cream involved in this invention does not irritate the skin and has no positive reaction.

[0116] Test Example 3

[0117] Stability test

[0118] The cream provided in Case 1 underwent accelerated stability testing for 3 months: accelerated stability test at 40℃, accelerated stability test at -16℃, accelerated stability test at 25℃, and accelerated stability test with daily cycles of 40℃ and -16℃. The results are shown in Table 6.

[0119] Table 6

[0120]

[0121] The results showed that under accelerated testing conditions for 3 months, the product's key indicators, including appearance, odor, and pH value, did not change significantly. Therefore, it can be seen that the system of this invention possesses excellent chemical stability and microbiological safety, meeting the requirements for commercial storage and trial use, and providing a solid foundation for its industrial production and market promotion.

[0122] Test Example 4

[0123] Soothing test

[0124] 120 volunteers, aged 18-50, were randomly divided into 12 groups of 10 each. Each volunteer applied an appropriate amount of the cream evenly to their entire face after cleansing in the morning and evening, continuing the treatment for 28 days. Soothing efficacy tests were conducted on volunteers before product use, after 14 days of use, and after 28 days of use to obtain skin... The value is calculated using the following formula to determine the rate of change after 14 and 28 days of product use:

[0125] In the formula, D0 represents the baseline skin parameters of the test area before product use; D14 represents the skin parameter values ​​of the test area after 14 days of product use; and D28 represents the skin parameter values ​​of the test area after 28 days of product use.

[0126] The soothing efficacy test used a Skin-Colorimeter CL400 (Courage & Khazaka, Germany) to measure the cheek area five times, collecting skin samples. value, The lower the value, the less redness the skin shows.

[0127] The test results are shown in Table 7.

[0128] Table 7

[0129]

[0130] The test results show that:

[0131] (1) As can be seen from Application Examples 1 to 6, the present invention combines lactic acid bacteria fermentation product, ethylhexylglycerin, polyol, bisabolol, lactobacillus / soy milk fermentation product filtrate and kava leaf / root / stem extract, and each component has a synergistic effect in providing soothing effects.

[0132] (2) By comparing Application Example 1 and Application Examples 4-6, it can be seen that the present invention can achieve better anti-inflammatory and soothing effects by further adopting a polyol system of butanediol, 1,2-hexanediol and 1,2-pentanediol.

[0133] (3) By comparing Application Example 1 and Comparative Application Examples 1-6, it can be seen that when any one of the following is missing from the composition: lactic acid bacteria fermentation product, ethylhexylglycerin, polyol, bisabolol, lactobacillus / soy milk fermentation product filtrate or kava pepper leaf / root / stem extract, the soothing effect is reduced.

[0134] The applicant declares that the above description is only a specific embodiment of the present invention, but the protection scope of the present invention is not limited thereto. Those skilled in the art should understand that any changes or substitutions that can be easily conceived by those skilled in the art within the technical scope disclosed in the present invention fall within the protection and disclosure scope of the present invention.

Claims

1. A cosmetic preservative composition, characterized in that, The cosmetic preservative composition includes lactic acid bacteria fermentation products, ethylhexylglycerin, polyols, bisabolol, lactobacillus / soybean milk fermentation product filtrate, and kava leaf / root / stem extract.

2. The cosmetic preservative composition according to claim 1, characterized in that, The cosmetic preservative composition comprises, by weight parts, 0.1-5 parts of lactic acid bacteria fermentation product, 0.05-1 parts of ethylhexylglycerin, 0.5-20 parts of polyol, 0.015-0.03 parts of bisabolol, 1-5 parts of lactic acid bacteria / soy milk fermentation product filtrate, and 1-5 parts of kava leaf / root / stem extract.

3. The cosmetic preservative composition according to claim 1 or 2, characterized in that, The polyol is a C4-C10 polyol.

4. The cosmetic preservative composition according to claim 3, characterized in that, The polyol includes any one or a combination of at least two of butanediol, 1,2-hexanediol, or 1,2-pentanediol.

5. The cosmetic preservative composition according to claim 4, characterized in that, The polyols include a combination of butanediol, 1,2-hexanediol and 1,2-pentanediol; Preferably, the mass ratio of butanediol, 1,2-hexanediol and 1,2-pentanediol is (1-10):(0.2-5):

1.

6. The use of a cosmetic preservative composition according to any one of claims 1-5 in cosmetics.

7. A cream, characterized in that, The cream comprises preservatives, antioxidants, moisturizers, emulsifiers, skin-nourishing agents, thickeners, pH neutralizers, repairing agents, and water, wherein the preservatives comprise the cosmetic preservative compositions according to any one of claims 1-5.

8. The cream according to claim 7, characterized in that, The cream comprises, by weight percentage, 0.3-25% preservatives, 0.1-10% antioxidants, 1-15% moisturizers, 1-10% emulsifiers, 3-30% skin moisturizers, 0.1-2% thickeners, 0.01-1% pH neutralizers, 0.1-10% repairing agents, and 50-70% water.

9. The cream according to claim 7 or 8, characterized in that, The antioxidants include any one or a combination of at least two of ubiquinone, tocopheryl acetate, or carnosine. Preferably, the humectant comprises any one or a combination of at least two of glycerol polyether-26, allantoin, betaine, biosaccharide gum-1, or sugar isomers; Preferably, the emulsifier comprises any one or a combination of at least two of the following: oil palm oil, oil palm kernel oil, behenol, polyglycerol-10 pentastearate, or sodium stearoyl lactylate. Preferably, the emollient comprises any one or a combination of at least two of isononyl isononanoate, shea butter, triglyceride (ethylhexanoate), squalane, or jojoba seed oil. Preferably, the thickener comprises any one or a combination of at least two of the following: sodium polyacrylamide dimethyl taurate, acrylate / C10-30 alkanol acrylate crosspolymer, or ammonium acrylamide dimethyl taurate / VP copolymer; Preferably, the pH neutralizing agent comprises any one or a combination of two of aminopropanol or triethanolamine; Preferably, the repairing agent comprises any one or a combination of at least two of the following: soft-haired pine algae extract, tetrahydromethylpyrimidine carboxylic acid, wheat flour lipids, or fibronectin.

10. A method for preparing a cream according to any one of claims 7-9, characterized in that, The preparation method includes the following steps: (1) Mix water, thickener and preservative polyol to obtain an aqueous phase; mix emollient and emulsifier to obtain an oil phase; (2) Mix and homogenize the aqueous phase and the oil phase, and then mix with the remaining components in the pH neutralizer, antioxidant, moisturizer, repair agent and preservative to obtain the cream.

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