Livestock and poultry antiviral growth-promoting composition based on traditional Chinese medicine extract and probiotics and preparation method of livestock and poultry antiviral growth-promoting composition

Abstract

The invention relates to the technical field of livestock and poultry breeding and veterinary drug preparations, and discloses an antiviral growth-promoting composition for livestock and poultry based on traditional Chinese medicine extracts and probiotics and a preparation method of the antiviral growth-promoting composition. The composition is prepared from astragalus membranaceus extract, isatis root extract, liquorice extract, probiotic freeze-dried powder, a fluidized bed carrier, a pH regulator and a secondary functionalizing agent according to specific parts by weight, and the preparation method comprises the following steps: constructing a traditional Chinese medicine solution, adding probiotics, and carrying out pre-incubation induction by linearly reducing the pH value, the suspension is sprayed into a fluidized bed for low-temperature spray drying granulation, particles are subjected to standing curing, and then surface hydrophobic modification is performed through gas phase adsorption of a secondary functionalizing agent. Through the pH gradient induction and fluidized bed drying technology, the acid resistance and heat resistance of probiotics are improved, and the moisture resistance and storage stability are improved through gas phase surface functionalization; the composition has the effects of resisting viruses and promoting growth, and solves the problem of biocompatibility of compounding of traditional Chinese medicines and probiotics.

Description

Technical Field

[0001] This invention relates to the fields of livestock and poultry breeding and veterinary drug formulation technology, specifically to a livestock and poultry antiviral growth-promoting composition based on traditional Chinese medicine extracts and probiotics, and its preparation method. Background Technology

[0002] With increasingly stringent restrictions on antibiotic use in livestock and poultry farming, the development of green and efficient antibiotic alternatives has become a key focus of the industry. Among current antibiotic alternatives, natural plant extracts (traditional Chinese medicine extracts) and microecological preparations (probiotics) are the two main directions. Traditional Chinese medicine extracts, such as Astragalus membranaceus and Isatis indigotica, have definite antiviral and immunomodulatory functions, but lack the ability to directly regulate intestinal flora and promote feed conversion. Probiotics can maintain intestinal flora balance and promote nutrient absorption, but their role in preventing viral infectious diseases is limited. Therefore, the combined use of the two to achieve the dual effects of antiviral and growth promotion has a clear clinical need.

[0003] However, existing technologies face technical obstacles when combining traditional Chinese medicine extracts with probiotics. Firstly, there is the issue of biocompatibility. Extracts from herbs such as Astragalus membranaceus and Isatis indigotica contain broad-spectrum antibacterial components. In liquid-phase mixing or high-humidity environments, these active ingredients can inhibit or even kill coexisting probiotics, leading to a rapid decline in the number of live bacteria in the product. Secondly, there is the issue of probiotic tolerance. Most Lactobacillus probiotics are sensitive to heat and acid. During the high-temperature pelleting process in feed processing and the highly acidic environment of the animal's stomach, their survival rate decreases due to a lack of effective protective mechanisms, preventing them from reaching the intestines and exerting their effects.

[0004] Furthermore, regarding formulation processes and storage stability, traditional Chinese medicine extracts typically exhibit strong hygroscopicity. If a simple physical mixing process is employed, the product is highly susceptible to moisture absorption during storage, leading to powder clumping. Increased water activity further accelerates the metabolism and death of probiotics, severely shortening the product's shelf life. Although existing technologies employ microencapsulation or granulation techniques to address these issues, conventional wet granulation often involves high-temperature drying, which can easily inactivate heat-sensitive strains. Low-temperature freeze-drying technology is costly and produces powders with poor flowability, making it difficult to meet the demands of large-scale industrial feed additives. Therefore, how to address the antagonistic effects between traditional Chinese medicine components and probiotics through process innovation, and achieve probiotic tolerance protection and moisture-proof stability of the finished product under conventional drying conditions, is a pressing technical challenge in this field. Summary of the Invention

[0005] To address the shortcomings of existing technologies, this invention provides a livestock and poultry antiviral and growth-promoting composition based on traditional Chinese medicine extracts and probiotics, and its preparation method. This solves the problems of poor biocompatibility, low survival rate of probiotics in the gastric acid environment, and significant loss of activity during processing in existing technologies where simply mixing traditional Chinese medicine extracts and probiotics results in a final product that cannot simultaneously achieve both antiviral and growth-promoting effects.

[0006] To address the above problems, the present invention provides the following technical solution: In a first aspect, the present invention provides a livestock and poultry antiviral and growth-promoting composition based on traditional Chinese medicine extracts and probiotics, comprising the following raw materials in parts by weight: 120-180 parts of Astragalus membranaceus extract; 60-90 parts of Isatis indigotica extract; 20-40 parts of Glycyrrhiza uralensis extract; 40-120 parts of freeze-dried probiotic powder; 200-600 parts of fluidized bed carrier; 50-300 parts of pH adjuster; and 0.05-1.0 parts of secondary functionalizing agent.

[0007] By employing the above-mentioned technical solution, Astragalus membranaceus extract, Isatis indigotica extract, and Glycyrrhiza uralensis extract work synergistically to exert antiviral and immunomodulatory effects; the probiotic freeze-dried powder promotes nutrient absorption by regulating the intestinal flora. The components are combined within a specific weight range, and the polysaccharides in the herbal extracts serve as a carbon source and protective matrix for the probiotics, maintaining strain activity and mitigating the potential for antibacterial components in the herbal medicines to inhibit probiotic growth.

[0008] Preferably, the probiotic freeze-dried powder is selected from Lactobacillus casei and Lactobacillus plantarum, and the preferred weight parts of the probiotic freeze-dried powder in the composition are 50-100 parts.

[0009] By adopting the above technical solution, the above-mentioned Lactobacillus strains are tolerant to the traditional Chinese medicine extracts in this formula, and the preferred weight range ensures the colonization quantity of the final product in the intestines of livestock and poultry, so as to achieve the growth promotion effect.

[0010] Preferably, the fluidized bed carrier is selected from one or a combination of two of maltodextrin and lactose, and the preferred weight parts of the fluidized bed carrier in the composition are 300-500 parts.

[0011] By adopting the above technical solution, maltodextrin and lactose, as water-soluble carriers, can assist the fluidized bed process in forming particles with uniform particle size, providing an attachment framework for active ingredients.

[0012] Preferably, the secondary functionalizing agent is selected from one or a combination of two of thymol and carvacrol, and the preferred weight part of the secondary functionalizing agent in the composition is 0.1-0.5 parts.

[0013] By adopting the above technical solution, trace amounts of phenolic substances are adsorbed on the surface of the particles to form a hydrophobic layer, which can block water vapor, prevent moisture absorption during storage, and inhibit the growth of bacteria on the surface of the particles.

[0014] Secondly, the present invention provides a method for preparing a livestock and poultry antiviral and growth-promoting composition based on traditional Chinese medicine extracts and probiotics, comprising the following steps: Prepare a basic solution of traditional Chinese medicine containing Astragalus membranaceus extract, Isatis indigotica extract and Glycyrrhiza uralensis extract; The temperature of the traditional Chinese medicine base solution is controlled, the initial pH value is adjusted, the probiotic freeze-dried powder is added and stirred for incubation, and then the pH adjuster is added dropwise at a constant flow rate while maintaining stirring. The pH value of the system is linearly reduced to the target value within a set time to obtain a pre-incubated composite suspension. The fluidized bed carrier is placed in the fluidized bed as the base material, and the pre-incubated composite suspension is sprayed into the fluidized bed for spray drying, and the particles are collected. The obtained particles were placed in a constant temperature and humidity environment for static maturation. The matured granules are transferred into a mixing device, and a vaporized secondary functionalizing agent is introduced for adsorption treatment, thus obtaining a combination of traditional Chinese medicine extract and probiotics for antiviral and growth-promoting effects in livestock and poultry.

[0015] By adopting the above technical solution, the present invention employs a liquid-phase induced complexation solid-phase adsorption preparation process, the mechanism of which is as follows: First, probiotics are added to the basic solution of traditional Chinese medicine, and then pre-incubated by linearly decreasing the pH value. This process simulates the acidic environment of the animal gastrointestinal tract, inducing the probiotics to initiate an acid tolerance response and increasing the activity of ATPases on the cell membrane and the expression of stress proteins. At the same time, polysaccharides and glycyrrhizic acid from the traditional Chinese medicine extract are adsorbed onto the surface of the probiotic cell wall under stirring, forming an encapsulation layer, which provides adaptive protection for the probiotics before they enter the drying process.

[0016] Next, the pre-incubated suspension is sprayed into a fluidized bed containing a carrier. Upon contact with the carrier, the atomized droplets evaporate, and the herbal extract precipitates out along with the carrier, encapsulating the probiotics within the particles. The low-temperature drying method minimizes thermal damage to the probiotics, ensuring the resolubility of the particles.

[0017] After the final granule maturation and shaping, a vaporized secondary functionalizing agent is introduced. The gaseous functionalizing agent permeates and adsorbs into the pores of the granule surface. This step does not introduce liquid solvents, thus avoiding granule moisture absorption. The phenolic substances adsorbed on the surface form a hydrophobic barrier, isolating external moisture and improving product storage stability. After the product is consumed, the surface antibacterial agent is released first, followed by granule disintegration releasing internal probiotics and traditional Chinese medicine components, achieving a staged release.

[0018] Preferably, the preparation method of the traditional Chinese medicine base solution includes: dissolving the Astragalus membranaceus extract, Isatis indigotica extract and Glycyrrhiza uralensis extract in purified water; stirring at room temperature until completely dissolved, and adjusting the volume to obtain a solution with a total extract solids concentration of 5.0%-5.17% (w / v).

[0019] By adopting the above technical solution, the solid concentration is controlled at 5.0%-5.17%, so that the solution has a suitable viscosity, which is conducive to the adsorption of Chinese medicine ingredients on the surface of probiotics, while avoiding wall adhesion or blockage during fluidized bed spraying.

[0020] Preferably, the process parameters of the pre-incubated composite suspension are: temperature control of 35.0-40.0℃; initial pH of 6.5-7.0; incubation time of 10-20 minutes; pH adjuster addition time of 25-35 minutes; and target pH reduction of 4.5-5.0.

[0021] By employing the above technical solution, the temperature range meets the metabolic conditions of probiotics; the pH value is lowered from neutral to 4.5-5.0 within 25-35 minutes, providing an appropriate induction time. This process induces the strain to produce stress proteins while avoiding bacterial damage caused by excessive time or excessively low pH.

[0022] Preferably, the process parameters for spray drying are: inlet air temperature of 50.0-60.0℃; atomization pressure of 0.15-0.25MPa; material temperature controlled at 35.0-42.0℃; and particle moisture content controlled at the drying endpoint below 5%.

[0023] By adopting the above technical solutions, the material temperature is controlled to not exceed 42℃ to prevent the probiotics from becoming inactive, and the moisture content is controlled to be below 5% to ensure the stability of the finished product during storage.

[0024] Preferably, the conditions for static curing are: temperature of 20.0-25.0℃; relative humidity of 40%-50%; and curing time of 8-12 hours.

[0025] By adopting the above technical solution, the curing process redistributes and balances the residual moisture inside the granules, eliminates internal stress, and prevents clumping or disintegration during subsequent packaging and storage.

[0026] Preferably, the adsorption treatment conditions for the secondary functionalizing agent are as follows: the secondary functionalizing agent is heated and vaporized, and then carried into the mixing device with an inert gas; the rotation speed of the mixing device is 8-12 rpm; and the adsorption time is 5-10 minutes.

[0027] By adopting the above technical solution, low-speed mixing and gas-phase loading are used to avoid particle breakage due to mechanical friction and to ensure uniform adsorption of functionalizing agents on the particle surface.

[0028] This invention provides a livestock and poultry antiviral and growth-promoting composition based on traditional Chinese medicine extracts and probiotics, and its preparation method. It has the following beneficial effects: 1. This invention improves the tolerance of probiotics to gastric acid and heat processing by combining pre-incubation with a linear pH gradient and fluidized bed spray drying. During the pre-incubation stage, the pH value is controlled to decrease linearly from 6.5-7.0 to 4.5-5.0 within a set time, inducing probiotics to initiate an acid tolerance response mechanism. At the same time, the polysaccharide components in the Chinese herbal extract form an embedding layer on the surface of the bacteria, thereby ensuring the number of live bacteria when the probiotics reach the intestine through the digestive tract.

[0029] 2. This invention improves the shelf-life stability of the composition through static curing and secondary surface functionalization in the gas phase. The static curing process allows the internal moisture of the particles to redistribute and reach equilibrium, reducing the internal stress of the particles. Subsequently, hydrophobic functionalizing agents such as thymol or carvacrol are modified on the particle surface using gas phase adsorption technology, which blocks the influence of environmental moisture on the probiotics inside the particles and solves the problem of easy moisture absorption and inactivation of microecological preparations during storage.

[0030] 3. This invention achieves growth promotion while preserving the antiviral activity of traditional Chinese medicine through a short-term stress co-treatment process. This process avoids the hydrolysis and inactivation of glycosides (such as glycyrrhizic acid) that may be caused by traditional long-term fermentation, and fully preserves the pharmacological material basis of the original medicine. In weaned piglet breeding, it improves daily weight gain and reduces diarrhea rate, and achieves synergistic effect between traditional Chinese medicine and probiotics. Attached Figure Description

[0031] Figure 1 This is a flowchart of the preparation process of the present invention. Detailed Implementation

[0032] The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the preparation examples, comparative examples, and test examples. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0033] Preparation Examples 1-3: Preparation Example 1: This preparation example provides a basic solution of traditional Chinese medicine, the preparation of which includes the following steps: (1) Weigh 150.0g of Astragalus membranaceus extract, 75.0g of Isatis indigotica extract and 30.0g of Glycyrrhiza uralensis extract and place them in a 5000mL beaker.

[0034] (2) Add about 3000 mL of purified water to the beaker, turn on the magnetic stirrer, and stir continuously for 60 minutes at room temperature until all the extract powder is completely dissolved, and a clear, brownish-yellow solution with no visible insoluble matter is obtained.

[0035] (3) Transfer the solution to a 5000mL volumetric flask, dilute to the mark with purified water, shake well, and the basic solution of traditional Chinese medicine is obtained. The total extract solids concentration in the solution is 5.1% (w / v), and it is ready for use.

[0036] Preparation Example 2: This preparation example provides a basic solution of traditional Chinese medicine, the preparation of which includes the following steps: (1) Weigh 120.0g of Astragalus membranaceus extract, 60.0g of Isatis indigotica extract and 20.0g of Glycyrrhiza uralensis extract and place them in a 5000mL beaker.

[0037] (2) Add about 3000 mL of purified water to the beaker, turn on the magnetic stirrer, and stir continuously for 60 minutes at room temperature until all the extract powder is completely dissolved, and a clear, brownish-yellow solution with no visible insoluble matter is obtained.

[0038] (3) Transfer the solution to a 4000mL volumetric flask, dilute to the mark with purified water, shake well, and the basic solution of traditional Chinese medicine is obtained. The total extract solids concentration in the solution is 5.0% (w / v), and it is ready for use.

[0039] Preparation Example 3: This preparation example provides a basic solution of traditional Chinese medicine, the preparation of which includes the following steps: (1) Weigh 180.0g of Astragalus membranaceus extract, 90.0g of Isatis indigotica extract and 40.0g of Glycyrrhiza uralensis extract and place them in a 10000mL beaker.

[0040] (2) Add about 5000 mL of purified water to the beaker, turn on the magnetic stirrer, and stir continuously for 60 minutes at room temperature until all the extract powder is completely dissolved, and a clear, brownish-yellow solution with no visible insoluble matter is obtained.

[0041] (3) Transfer the solution to a 6000mL volumetric flask, dilute to the mark with purified water, and shake well to obtain the basic Chinese medicine solution. The total extract solids concentration in this solution is approximately 5.17% (w / v). (Where the weight ratio is 1:1). Examples 1-4: Example 1: This embodiment provides a method for preparing a livestock and poultry antiviral and growth-promoting composition based on traditional Chinese medicine extracts and probiotics, including the following steps: (1) Take the Chinese medicine base solution (containing a total dry weight of 255.0g of Chinese medicine extract) prepared in Preparation Example 1, and separately weigh 75.0g of probiotic freeze-dried powder (the probiotic freeze-dried powder is one of Lactobacillus casei or Lactobacillus plantarum), 400.0g of fluidized bed carrier maltodextrin, 0.3g of secondary functionalizing agent thymol, and prepare a 10% (w / v) food-grade lactic acid solution as a pH adjuster.

[0042] (2) Place the basic Chinese medicine solution in a 10L jacketed incubator, turn on the water circulation heating, and control the solution temperature at 37.0℃. While stirring (40rpm), use 1mol / L NaOH solution to adjust the initial pH of the system to 6.8. Sprinkle 75.0g of probiotic freeze-dried powder evenly into the solution, maintain the above temperature and pH conditions for incubation for 15 minutes. Then, add 10% lactic acid solution dropwise into the tank at a constant flow rate using a peristaltic pump and continue stirring. Within 30 minutes, the pH value of the system is linearly reduced from 6.8 to 4.8. After the dropwise addition is completed, a total of 200.0g of 10% lactic acid solution is consumed, and a pre-incubated composite suspension is obtained. Proceed to the next step immediately.

[0043] (3) 400.0g of maltodextrin was put into the material chamber of the low-temperature fluidized bed dryer as the base material. The air inlet temperature was set to 55.0℃. The frequency of the induced draft fan was adjusted to make the material in a good fluidized state. The peristaltic pump and atomizer were started to spray the obtained composite suspension into the fluidized bed (atomization pressure 0.20MPa). During the spraying process, the material temperature was controlled to be maintained at about 40.0℃. After the liquid was sprayed, the fluidized drying continued for 5 minutes until the moisture content of the particles was less than 5%. The light yellow particles were collected.

[0044] (4) Spread the collected particles evenly in a tray and place them in a constant temperature and humidity chamber. Let them stand and mature for 10 hours at a temperature of 22.5℃ and a relative humidity of 45%. After maturation, transfer the particles into a V-type mixer, heat and vaporize 0.3g of thymol, and let it flow into the mixer with nitrogen. Adsorb for 7 minutes while the mixer is rotating (10rpm). Discharge the material, vacuum package it, and the finished product of the composition is obtained.

[0045] Example 2: This embodiment provides a method for preparing a livestock and poultry antiviral and growth-promoting composition based on traditional Chinese medicine extracts and probiotics, including the following steps: (1) Take the Chinese medicine base solution prepared in Preparation Example 2, and separately weigh 50.0g of probiotic freeze-dried powder, 300.0g of fluidized bed carrier lactose, 0.1g of secondary functionalizing agent carvacrol, and prepare 10% (w / v) food grade citric acid solution as pH adjuster.

[0046] (2) Place the basic Chinese medicine solution in a 10L incubator and control the temperature to 35.0℃. Adjust the initial pH of the system to 6.5, add 50.0g of probiotic freeze-dried powder, stir and incubate for 10 minutes. Then, add 10% citric acid solution dropwise and continue stirring. Within 25 minutes, the pH of the system is linearly reduced from 6.5 to 5.0, consuming a total of 120.0g of 10% citric acid solution, to obtain a pre-incubated composite suspension.

[0047] (3) Add 300.0g of lactose to the fluidized bed as the base material, set the air inlet temperature to 50.0℃ and the atomization pressure to 0.15MPa, spray the composite suspension into the fluidized bed, control the material temperature to be maintained at about 35.0℃, dry until the moisture content meets the standard, and collect the particles.

[0048] (4) Place the granules in a constant temperature and humidity chamber and let them stand for 8 hours at a temperature of 20.0℃ and a relative humidity of 40%. After vaporizing 0.1g of carvacrol, pass it into a V-type mixer and adsorb it for 5 minutes while the mixer is rotating (8rpm). Discharge the material and vacuum package it to obtain the finished product of the composition.

[0049] Example 3: This embodiment provides a method for preparing a livestock and poultry antiviral and growth-promoting composition based on traditional Chinese medicine extracts and probiotics, including the following steps: (1) Take the Chinese medicine base solution prepared in Preparation Example 3, and separately weigh 100.0g of probiotic freeze-dried powder, 500.0g of fluidized bed carrier maltodextrin, 0.5g of secondary functionalizing agent thymol, and prepare a 10% (w / v) food-grade lactic acid solution as a pH adjuster.

[0050] (2) Place the basic Chinese medicine solution in a 10L incubator, control the temperature to 40.0℃, adjust the initial pH of the system to 7.0, add 100.0g of probiotic freeze-dried powder, stir and incubate for 20 minutes, then add 10% lactic acid solution dropwise and continue stirring. Within 35 minutes, the pH value of the system is linearly reduced from 7.0 to 4.5, consuming a total of 280.0g of 10% lactic acid solution, and a pre-incubated composite suspension is obtained.

[0051] (3) Add 500.0g of maltodextrin to the fluidized bed as the base material, set the air inlet temperature to 60.0℃ and the atomization pressure to 0.25MPa, spray the composite suspension into the fluidized bed, control the material temperature to be maintained at about 42.0℃, dry until the moisture content meets the standard, and collect the particles.

[0052] (4) Place the granules in a constant temperature and humidity chamber and let them stand for 12 hours at a temperature of 25.0℃ and a relative humidity of 50%. After vaporizing 0.5g of thymol, pass it into a V-type mixer and adsorb it for 10 minutes while the mixer is rotating (12rpm). Discharge the material and vacuum package it to obtain the finished product of the composition.

[0053] Example 4: This embodiment provides a method for preparing a livestock and poultry antiviral and growth-promoting composition based on traditional Chinese medicine extracts and probiotics, including the following steps: (1) Take the Chinese medicine base solution prepared in Example 1, and separately weigh 80.0g of probiotic freeze-dried powder, fluidized bed carrier (a mixture of 200.0g of maltodextrin and 200.0g of lactose), 0.2g of secondary functionalizing agent carvacrol, and prepare 10% (w / v) food-grade citric acid solution as pH adjuster.

[0054] (2) Place the basic Chinese medicine solution in a 10L incubator, control the temperature to 38.0℃, adjust the initial pH of the system to 6.9, add 80.0g of probiotic freeze-dried powder, stir and incubate for 18 minutes, then add 10% citric acid solution dropwise and continue stirring. Within 28 minutes, the pH value of the system is linearly reduced from 6.9 to 4.6, consuming a total of 180.0g of 10% citric acid solution, and a pre-incubated composite suspension is obtained.

[0055] (3) The mixed carrier of maltodextrin and lactose is put into the fluidized bed, the air inlet temperature is set to 58.0℃ and the atomization pressure is 0.22MPa. The composite suspension is sprayed into the fluidized bed, the material temperature is controlled to be maintained at about 38.0℃, and the material is dried until the moisture content meets the standard and the particles are collected.

[0056] (4) Place the granules in a constant temperature and humidity chamber and let them stand for 11 hours at a temperature of 24.0℃ and a relative humidity of 42%. After vaporizing 0.2g of carvacrol, pass it into a V-type mixer and adsorb it for 8 minutes while the mixer is rotating (10rpm). Discharge the material and vacuum package it to obtain the finished product of the composition.

[0057] Comparative Examples 1-6: Comparative Example 1: This comparative example provides a livestock and poultry antiviral and growth-promoting composition based on traditional Chinese medicine extracts and probiotics. The difference from Example 1 is that wet co-processing and spray drying processes are not used; instead, powder mixing is performed directly. All other aspects are the same.

[0058] Comparative Example 2: This comparative example provides a livestock and poultry antiviral growth-promoting composition based on Chinese herbal extracts and probiotics. The difference between this example and Example 1 is that the only difference is the pre-incubation conditions in step (2), while the rest are the same.

[0059] Comparative Example 3: This comparative example provides a livestock and poultry antiviral growth-promoting composition based on Chinese herbal extracts and probiotics. Compared with Example 1, the difference is that only the pre-incubation process in step (2) is omitted, and the rest are the same.

[0060] Comparative Example 4: This comparative example provides a livestock and poultry antiviral growth-promoting composition based on Chinese herbal extracts and probiotics. Compared with Example 1, the only difference is that the constant humidity ripening process in step (4) is omitted, while the rest are the same.

[0061] Comparative Example 5: This comparative example provides a livestock and poultry antiviral and growth-promoting composition based on Chinese herbal extracts and probiotics. Compared with Example 1, the difference is that only the adsorption operation of thymol in step (4) is omitted, and the rest are the same.

[0062] Comparative Example 6: This comparative example provides a livestock and poultry antiviral growth-promoting composition based on Chinese herbal extracts and probiotics. Compared with Example 1, the difference is that only the processing time and mechanism of step (2) are different, while the rest are the same.

[0063] Test Example 1-3: Test Example 1: This test example verifies the stress resistance and storage stability of the probiotics in the composition. The test samples were the compositions prepared in Examples 1-4 and Comparative Examples 1-6. The total viable count was determined by plate counting according to GB 4789.35-2016 standard.

[0064] Experimental steps: A simulated gastric fluid with pH 2.0 and containing 10 g / L pepsin was prepared and sterilized by passing it through a 0.22 μm filter membrane. 1.0 g of each sample was added to 9.0 mL of the simulated gastric fluid and incubated at 37 °C in a shaker for 2 hours. After treatment, the solution was diluted with phosphate buffer and the number of viable bacteria was determined.

[0065] Each group of samples was sealed and placed in an 80°C water bath for 5 minutes. After being removed and cooled to room temperature, the number of viable bacteria was determined.

[0066] Each group of samples was sealed and stored in an environment of 25°C and 60% relative humidity. Samples were taken in the 6th month to determine the number of viable bacteria and calculate the retention rate.

[0067] Experimental data: Table 1: Tests on the stress resistance and storage stability of probiotics The test results are summarized as follows: Table 1 shows that the survival rate of Examples 1-4 in simulated gastric fluid environment was higher than 80%, while the survival rates of Comparative Examples 2 and 3, which were not subjected to pH stress treatment, were in the range of 40%-46%. Although Comparative Example 6 used long-term fermentation and had a high initial viable cell count, its survival rate under simulated gastric fluid and high-temperature conditions was lower than that of the Example groups. The data indicate that pH gradient stress operation induced an adaptation of the cells to acidic and thermal environments, which was superior to simple logarithmic growth or the untreated state.

[0068] Table 1 shows that the viable cell retention rate in the example group exceeded 90%, which was higher than that of Comparative Example 4 (75.4%) without post-curing and Comparative Example 5 (79.2%) without secondary functionalization. Comparative Example 1 (physical mixing) had the lowest retention rate (68.3%). The data confirms that the post-curing step reduced the water activity inside the particles, and the hydrophobic layer formed by secondary functionalization blocked environmental moisture. The combined effect of these two factors improved the long-term stability of the product.

[0069] Test Example 2: This test case verifies in vitro antiviral activity against porcine transmissible gastroenteritis virus (TGEV). The experiment used ST cells and the TGEVWH-1 strain. Experimental procedures were as follows: Take samples from each group, grind them, and dissolve them in serum-free DMEM medium to prepare... L solution, after 0.22 The membrane filter is used for sterilization, the maximum non-toxic concentration is determined, and experiments are conducted at that concentration.

[0070] ST cells were seeded into 96-well plates to grow into a monolayer. After aspirating the culture medium, 100 TCID45 was added. 50 The TGEV virus liquid was adsorbed for 1 hour.

[0071] Discard the virus solution and add maintenance medium containing the drug. Set up a normal cell control group and a virus control group. After incubation at 37℃ and 5% CO2 for 48 hours, add MTT solution for staining and measure OD. 490 The value was then calculated to determine the virus inhibition rate.

[0072] Table 2: Results of in vitro anti-TGEV virus activity test Note: "-" indicates that no test sample was added.

[0073] The test results are summarized as follows: Table 2 shows that the inhibition rate of the Example Group against TGEV virus was close to 90%, which was higher than that of Comparative Example 1 (62.3%), which was physically mixed. The virus inhibition rate of Comparative Example 6 was 55.7%, which was lower than that of the Example Group and Comparative Example 1. These results suggest that the long-term fermentation process may lead to the hydrolysis of glycosides (such as glycyrrhizic acid and quercetin) in the traditional Chinese medicine extract, reducing the antiviral activity of the original drug; while the short-term stress co-treatment process used in this invention preserves the structural integrity of the traditional Chinese medicine components.

[0074] Test Example 3: This test case validated the growth performance and diarrhea rate of weaned piglets. Two hundred and forty 21-day-old weaned piglets weighing 6.5 ± 0.5 kg were randomly divided into eight groups, with three replicates per group and ten piglets per replicate. The blank control group was fed a basal diet, the positive control group was fed a basal diet supplemented with 20 mg / kg colistin sulfate, and the experimental groups were fed a basal diet supplemented with 1.0 kg / ton of the corresponding compound. The experiment lasted for 28 days, with free access to feed and water. Initial weight, final weight, feed consumption, and the number of piglets with diarrhea per day were recorded. Average daily gain (ADG), feed conversion ratio (F / G), and diarrhea rate were calculated.

[0075] Table 3: Results of tests on growth performance and diarrhea rate in weaned piglets The test results are summarized as follows: As shown in Table 3, the average daily weight gain of the Example groups (1-4) was better than that of the blank control group and the antibiotic positive control group. Among them, Example 3 (high dose / high protection process) showed the best growth-promoting effect (380.35 g / d) and the lowest diarrhea rate (2.25%). The results indicate that the composition prepared by the process of the present invention has application value in improving the growth performance and anti-diarrheal properties of piglets, and its effect is better than that of simple physical compositions.

Claims

1. A composition for antiviral and growth-promoting effects in livestock and poultry based on traditional Chinese medicine extracts and probiotics, characterized in that, Made from the following ingredients in parts by weight: Astragalus extract: 120-180 parts; Isatis root extract: 60-90 parts; Licorice extract: 20-40 parts; Probiotic freeze-dried powder: 40-120 servings; Fluidized bed carrier: 200-600 parts; pH adjuster: 50-300 parts; Secondary functionalizing agent: 0.05-1.0 parts.

2. The antiviral and growth-promoting composition for livestock and poultry based on traditional Chinese medicine extracts and probiotics according to claim 1, characterized in that, The probiotic freeze-dried powder is one of Lactobacillus casei or Lactobacillus plantarum, and the preferred weight part of the probiotic freeze-dried powder in the composition is 50-100 parts.

3. The antiviral and growth-promoting composition for livestock and poultry based on traditional Chinese medicine extracts and probiotics according to claim 1, characterized in that, The fluidized bed carrier is selected from maltodextrin or lactose, and the preferred weight parts of the fluidized bed carrier in the composition are 300-500 parts.

4. The antiviral and growth-promoting composition for livestock and poultry based on traditional Chinese medicine extracts and probiotics according to claim 1, characterized in that, The secondary functionalizing agent is selected from thymol or carvacrol, and the preferred weight part of the secondary functionalizing agent in the composition is 0.1-0.5 parts.

5. A method for preparing a livestock and poultry antiviral and growth-promoting composition based on traditional Chinese medicine extracts and probiotics, characterized in that, The application of a livestock and poultry antiviral and growth-promoting composition based on traditional Chinese medicine extracts and probiotics as described in any one of claims 1-4 includes the following steps: Prepare a basic solution of traditional Chinese medicine containing Astragalus membranaceus extract, Isatis indigotica extract and Glycyrrhiza uralensis extract; The temperature of the traditional Chinese medicine base solution is controlled, the initial pH value is adjusted, the probiotic freeze-dried powder is added and stirred for incubation, and then the pH adjuster is added dropwise at a constant flow rate while maintaining stirring. The pH value of the system is linearly reduced to the target value within a set time to obtain a pre-incubated composite suspension. The fluidized bed carrier is placed in the fluidized bed as the base material, and the pre-incubated composite suspension is sprayed into the fluidized bed for spray drying, and the particles are collected. The obtained granules are placed in a constant temperature and humidity environment for static maturation to obtain matured granules; the matured granules are transferred into a mixing device and a vaporized secondary functionalizing agent is introduced for adsorption treatment to obtain a livestock and poultry antiviral and growth-promoting composition of traditional Chinese medicine extract and probiotics.

6. The method for preparing a livestock and poultry antiviral and growth-promoting composition based on traditional Chinese medicine extracts and probiotics according to claim 5, characterized in that, The preparation method of the basic Chinese medicine solution specifically includes the following steps: The Astragalus extract, Isatis root extract and licorice extract were dissolved in purified water; Stir until completely dissolved at room temperature, and bring to a final volume to obtain a basic Chinese medicine solution with a total extract solids concentration of 5.0%-5.17% (w / v).

7. The method for preparing a livestock and poultry antiviral and growth-promoting composition based on traditional Chinese medicine extracts and probiotics according to claim 5, characterized in that, The process parameters for the pre-incubated composite suspension are as follows: The temperature control range is 35.0-40.0℃; The initial pH value is 6.5-7.0; Incubation time is 10-20 minutes; The pH adjuster should be added over 25-35 minutes. The target value for reducing the pH value is 4.5-5.

0.

8. The method for preparing a livestock and poultry antiviral and growth-promoting composition based on traditional Chinese medicine extracts and probiotics according to claim 5, characterized in that, The process parameters for spray drying are as follows: The inlet air temperature is 50.0-60.0℃; The atomization pressure is 0.15-0.25 MPa; The material temperature is controlled between 35.0-42.0℃; The drying endpoint is controlled so that the moisture content of the particles is below 5%.

9. The method for preparing a livestock and poultry antiviral and growth-promoting composition based on traditional Chinese medicine extracts and probiotics according to claim 5, characterized in that, The conditions for the static ripening process are as follows: The temperature is 20.0-25.0℃; Relative humidity is 40%-50%; The maturation time is 8-12 hours.

10. The method for preparing a livestock and poultry antiviral and growth-promoting composition based on traditional Chinese medicine extracts and probiotics according to claim 5, characterized in that, The adsorption treatment conditions for the secondary functionalizing agent are as follows: The secondary functionalizing agent is heated and vaporized, and then carried into the mixing equipment along with an inert gas; The mixing equipment operates at a speed of 8-12 rpm; The adsorption time is 5-10 minutes.

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