Herbal skin antibacterial gel containing olive leaf extract as well as preparation method and application of herbal skin antibacterial gel

Abstract

The invention discloses herbal skin antibacterial gel containing olive leaf extract as well as a preparation method and application of the herbal skin antibacterial gel, and belongs to the technical field of external antibacterial preparations for skin. The antibacterial gel is prepared from the following raw materials in percentage by mass: 1%-10% of an olive leaf extract, 0.1%-0.2% of menthol, 2.6%-12.3% of a hydrogel matrix, a proper amount of a pH regulator and the balance of water, wherein the hydrogel matrix is prepared from carbomer, sodium hyaluronate and butanediol. The preparation method comprises the following steps: dissolving the olive leaf extract and the menthol to prepare a phase A, mixing the matrix components to prepare a phase B, mixing the two phases, and regulating the pH value of the system to 5.0-7.0 by using the pH regulator. The gel has a remarkable broad-spectrum antibacterial effect on common skin pathogenic bacteria such as staphylococcus aureus, escherichia coli and candida albicans, is non-irritant to skin and good in stability, and is suitable for inhibiting bacteria and fungi on the surface of the skin and relieving skin discomfort caused by mosquito bites and the like.

Description

Technical Field

[0001] This invention belongs to the field of topical antibacterial agents for skin, specifically relating to an herbal antibacterial gel containing olive leaf extract, its preparation method, and its application. Background Technology

[0002] The skin is the body's first line of defense against the external environment. In daily work and life, itching, redness, swelling, and pain are common discomforts caused by insect bites, minor injuries, or environmental irritants. Scratching can damage the skin barrier, leading to secondary infections by bacteria, fungi, and other microorganisms, causing or worsening skin inflammation. Therefore, there is a continuous market demand for safe, effective, and fast-relieving antibacterial products.

[0003] Currently, most common skin antibacterial and disinfectant products on the market are chemical disinfectants (such as alcohols, quaternary ammonium salts, and povidone-iodine). While these products have a clear antibacterial effect, long-term or frequent use may cause dry skin, irritation, or allergic reactions, and there is a potential risk of chemical residues. Furthermore, overuse of chemical disinfectants may disrupt the normal microecological balance of the skin.

[0004] As consumers increasingly favor natural and safe products, herbal antibacterial agents based on plant extracts are gaining attention. For example, olive oil (… Olea europaea L. leaf extract has been shown to be rich in polyphenolic active substances such as oleuropein and hydroxytyrosol, possessing broad-spectrum antibacterial, anti-inflammatory, and antioxidant properties, making it a promising natural antibacterial ingredient. However, developing plant extracts into stable, effective, and user-friendly topical formulations still faces challenges: firstly, the antibacterial spectrum and efficacy of single plant extracts are sometimes insufficient; secondly, the addition of high-concentration active ingredients may affect the stability of the formulation or cause irritation; and thirdly, effectively delivering active ingredients to the skin application site while maintaining a pleasant skin feel (such as cooling, soothing, and non-sticky) is a challenge in formulation design.

[0005] While some patented technologies involve antibacterial compositions containing plant extracts, most still have certain limitations. For example, there is an existing antibacterial composition mainly used for gynecological cavity infections (such as Chinese patent publication number CN119345292A), which uses a complex compound of multiple plants including olive leaf extract, supplemented by a special magnetic microsphere purification process and antimicrobial peptides. Although this technical solution is designed for a specific cavity environment and emphasizes the balance of flora, its ingredients are complex, the process is cumbersome, and the cost is high. Moreover, its dosage form and formulation design mainly focus on the mucosal administration environment, which differs significantly from topical skin care in terms of dosage form requirements, usage scenarios, comfort standards, and core concerns (such as rapid itch relief, cooling sensation, and daily protection). It does not provide a gel formulation that is suitable for daily skin care, has a simplified formula, is safe and convenient to use, and is optimized for common skin problems.

[0006] Therefore, there is an urgent need in this field to develop a new antibacterial product that possesses the following characteristics: 1) a simplified formula based on recognized and effective natural antibacterial ingredients; 2) a dosage form suitable for topical skin use, stable and with a pleasant skin feel; 3) rapid soothing and antipruritic effects and a clear broad-spectrum antibacterial ability; and 4) gentle and non-irritating to the skin, suitable for repeated daily skincare. This invention is based on the aforementioned unmet needs. Summary of the Invention

[0007] To address the shortcomings of existing antibacterial skin products in terms of safety, skin feel, formulation complexity, and compatibility with skincare, this invention aims to provide a simplified, gentle, effective, and user-friendly topical antibacterial gel. Specific objectives include: First, we offer an antibacterial gel with a clearly defined matrix component and a core of highly active olive leaf extract. Its formula is optimized for skin care and eliminates unnecessary complex compounding.

[0008] Secondly, a simple and stable preparation method for this gel is provided.

[0009] Finally, the application of this gel in broad-spectrum antibacterial activity on the skin surface and in relieving discomfort symptoms such as mosquito bites is provided.

[0010] To achieve the above-mentioned objectives, the present invention adopts the following technical solution: In a first aspect, the present invention provides an herbal antibacterial skin gel, made from the following raw materials in weight percentages: Olive leaf extract 1%-10%; Menthol 0.1%-0.2%; Hydrogel matrix 2.6%-12.3%; pH adjuster: appropriate amount; Water: Balance; The hydrogel matrix is ​​composed of carbomer, sodium hyaluronate and butylene glycol, wherein carbomer accounts for 0.5% - 2.0% of the total mass of the gel, sodium hyaluronate accounts for 0.1% - 0.3% and butylene glycol accounts for 2% - 10%.

[0011] Preferably, the pH adjuster is triethanolamine.

[0012] Preferably, the pH of the gel is adjusted to 5.0-7.0, which is closer to the slightly acidic environment of healthy skin, making it gentle and non-irritating.

[0013] Preferably, the olive leaf extract contains no less than 50% oleuropein to ensure core antibacterial activity.

[0014] Preferably, the addition of menthol (CAS: 89-78-1) not only provides an immediate cooling and antipruritic sensation and improves the user experience, but its penetration-enhancing effect can also synergize with olive leaf extract to enhance the overall antibacterial effect.

[0015] Secondly, the present invention provides a method for preparing the above-mentioned herbal antibacterial skin gel, comprising the following steps: (1) Dissolve the prescribed amount of olive leaf extract and menthol in a portion of water, stir until completely dissolved, and prepare phase A; (2) Premix the formulated amounts of butanediol, carbomer, and sodium hyaluronate in the remaining water or add them sequentially, and stir until they are evenly dispersed to form a uniform phase B (the prototype of the hydrogel matrix). (3) Under continuous stirring (preferably vacuum stirring to reduce bubbles), the A phase is slowly added to or dispersed into the B phase to ensure that the active ingredients and the matrix are fully and evenly mixed. (4) Add a pH adjuster (such as triethanolamine) while stirring until the pH of the system is stably adjusted to the target range of 5.0-7.0, and a gel is formed; (5) Fill and seal the obtained gel to obtain the finished product.

[0016] Thirdly, the present invention provides the application of the herbal antibacterial skin gel, specifically its application in the preparation of products for inhibiting pathogenic microorganisms on the skin surface or relieving skin discomfort.

[0017] Furthermore, the pathogenic microorganisms include, but are not limited to, Staphylococcus aureus, Escherichia coli, and Candida albicans.

[0018] Furthermore, the skin discomfort includes itching, redness, or allergies caused by insect bites.

[0019] This invention creatively combines olive leaf extract and peppermint extract in an herbal antibacterial skin gel, producing a significant synergistic antibacterial effect: 1. Complementary Targets: Peppermint extract primarily acts on the cell membrane, rapidly disrupting its barrier function; high-concentration olive leaf extract, on the other hand, goes a step further, disrupting the cell wall / membrane structure, inhibiting biofilm formation, interfering with intracellular enzyme activity and energy metabolism, and even inducing oxidative stress. Together, they form a multi-target, multi-layered antibacterial network, from external to internal action, from physical disruption to biochemical interference.

[0020] 2. Broad-spectrum and highly effective antibacterial: This composition exhibits excellent inhibitory and bactericidal effects against common skin pathogens, including Gram-positive bacteria (such as Staphylococcus aureus, Staphylococcus epidermidis, and Propionibacterium acnes), Gram-negative bacteria (such as Escherichia coli and Pseudomonas aeruginosa), and fungi (such as Candida albicans and Malassezia), effectively preventing and adjuvantly treating skin infections and inflammations caused by these microorganisms.

[0021] 3. Safe and gentle: The main active ingredients are derived from natural plants, which greatly reduces the risk of skin irritation, allergies and drug resistance, making it suitable for sensitive skin and long-term use.

[0022] 4. Additional benefits: The cooling sensation provided by menthol can soothe skin discomfort; the antioxidant and anti-inflammatory properties of olive leaf extract help reduce skin inflammation and promote skin health.

[0023] In summary, the product of this invention can effectively relieve itching and alleviate allergies caused by mosquito bites. Attached Figure Description

[0024] Figure 1 This is a schematic diagram illustrating the antibacterial effects of the example and comparative products against Staphylococcus aureus.

[0025] Figure 2 This is a schematic diagram illustrating the antibacterial effect of the example and comparative products against Candida albicans.

[0026] Figure 3 This is a schematic diagram illustrating the antibacterial effects of the example and comparative products on Escherichia coli.

[0027] Figure 4 The bar chart shows the average inhibition rate of each example and comparative product against the three test bacteria.

[0028] Figure 5 This is a photograph of the actual product of the olive leaf extract herbal antibacterial skin gel described in this invention. Detailed Implementation

[0029] To make the objectives, technical solutions, and advantages of this invention clearer, the invention will be further described in detail below with reference to specific embodiments and experimental data. It should be understood that the specific embodiments described herein are for illustrative purposes only and do not constitute a limitation thereof. All other embodiments obtained by those skilled in the art based on the embodiments of this invention without inventive effort are within the scope of protection of this invention.

[0030] Example 1 The product comprises, by weight percentage, 0.2% olive leaf extract (90% oleuropein content), 0.1%-0.2% menthol, 2.6%-12.3% hydrogel matrix (0.5%-2.0% carbomer, 0.1%-0.3% sodium hyaluronate, 2%-10% butylene glycol), and the remainder being purified water. The olive leaf extract and menthol are dissolved in half the total water volume to prepare aqueous phase A. The butylene glycol, carbomer, and sodium hyaluronate are mixed to form the hydrogel matrix, forming phase B. Phase A is uniformly dispersed into phase B under vacuum stirring. The pH is adjusted to 5.0-7.0 using triethanolamine. The gel is then filled, sealed, and packaged to obtain herbal antibacterial skin gel product A.

[0031] Example 2 This embodiment relates to the antibacterial skin gel described in this application, which is prepared from raw materials comprising the following parts by weight: The following components are present by weight percentage: 1% olive leaf extract (50% oleuropein content), 0.1%-0.2% menthol; 2.6%-12.3% hydrogel matrix (0.5%-2.0% carbomer, 0.1%-0.3% sodium hyaluronate, 2%-10% butylene glycol); and the remainder is purified water. The olive leaf extract and menthol are dissolved in half the total water volume to prepare aqueous phase A. The butylene glycol, carbomer, and sodium hyaluronate are mixed to prepare the hydrogel matrix, forming phase B. Phase A is uniformly dispersed into phase B under vacuum stirring. The pH is adjusted to 5.0-7.0 using triethanolamine. The gel is then filled, sealed, and packaged to obtain herbal antibacterial skin gel product B.

[0032] Example 3 This embodiment relates to the antibacterial skin gel described in this application, which is prepared from raw materials comprising the following parts by weight: The following components are added by weight percentage: 10% olive leaf extract (50% oleuropein content), 0.1%-0.2% menthol; 2.6%-12.3% hydrogel matrix (0.5%-2.0% carbomer, 0.1%-0.3% sodium hyaluronate, 2%-10% butylene glycol); and the remainder is purified water. The olive leaf extract and menthol are dissolved in half the total water volume to prepare aqueous phase A. The butylene glycol, carbomer, and sodium hyaluronate are mixed to prepare the hydrogel matrix, forming phase B. Phase A is uniformly dispersed into phase B under vacuum stirring. The pH is adjusted to 5.0-7.0 using triethanolamine. The gel is then filled, sealed, and packaged to obtain herbal antibacterial skin gel product C.

[0033] Comparative Example 1 Yingxing® Herbal Antibacterial Gel The product being compared is a commercially available product, classified as a Class II disinfectant, used for skin antibacterial (no-rinse), antipruritic, and relief of discomfort from mosquito bites.

[0034] Performance evaluation was conducted on the products of Examples 1-3 and the comparative examples: I. Antibacterial Experiment 1. Staphylococcus aureus antibacterial effect such as Figure 1 As shown (A-Control, B-Example 1, C-Example 2, D-Example 3, E-Comparative Example 1): 10% OELE gel (Example 3) > Yingxing product (Comparative Example 1) > 1% OELE gel (Example 2) > 0.2% OELE gel (Example 1) > Blank matrix.

[0035] 2. The antibacterial effect of Candida albicans is as follows: Figure 2 As shown (A-Control, B-Example 1, C-Example 2, D-Example 3, E-Comparative Example 1): 10% OELE gel (Example 3) ≈ Yingxing product (Comparative Example 1) > 1% OELE gel (Example 2) > 0.2% OELE gel (Example 1) > Blank matrix.

[0036] 3. Escherichia coli antibacterial effect such as Figure 3 As shown (A-Control, B-Example 1, C-Example 2, D-Example 3, E-Comparative Example 1): 10% OELE gel (Example 3) ≈ Yingxing product (Comparative Example 1) > 1% OELE gel (Example 2) > 0.2% OELE gel (Example 1) > Blank matrix.

[0037] Conclusion: The optimal antibacterial gel formulation is the addition of 10% olive leaf extract with a 50% oleuropein content (i.e., Example 3).

[0038] II. Repeated skin irritation tests 1. Materials and Strains (1) Test sample: 50% olive leaf extract (OELE) herbal antibacterial gel of the present invention (2) Experimental animals: 4 Japanese rabbits, half male and half female, weighing 2.0-2.5 kg, license number: SCXK(Shaanxi)2014-003, feed and bedding source as above.

[0039] (3) Animal room environment: temperature: 22-25℃, relative humidity: 45%-55%.

[0040] 2. Method (1) Testing basis: The testing method is based on the 2002 edition of the National Health Commission's "Disinfection Technical Specifications".

[0041] (2) Test substance dosage: 0.5 g / animal.

[0042] (3) Experimental method: 24 hours before the experiment, the fur on both sides of the spine on the back of each rabbit was clipped and the skin was prepared, with a hair removal area of ​​3×6cm on each side. 2 Take 0.5 g of the test substance per rabbit and apply it evenly to one side of the skin of each rabbit once a day. Use the other side as a solvent control. Continue application for 14 consecutive days. Trim the hair before each application, ensuring no skin damage and guaranteeing full contact between the test substance and the skin. Observe the skin reaction and score it daily. At the end of the experiment, collect skin samples from the application site for histopathological examination.

[0043] 3. Results The rabbits in the test group showed no erythema or edema on their skin, as shown in Table 1.

[0044] Table 1. Results of repeated skin irritation experiments on rabbits using "Olive Leaf Extract (OELE) Herbal Antibacterial Skin Gel". 4. Conclusion The average skin irritation score of "Olive Leaf Extract (OELE) Herbal Antibacterial Skin Gel" in rabbits was 0 after multiple skin irritation reactions. According to the skin irritation reaction grading standard in the 2002 edition of the "Disinfection Technical Specifications" issued by the National Health Commission, "Olive Leaf Extract (OELE) Herbal Antibacterial Skin Gel" is considered non-irritating to the skin of rabbits.

[0045] III. Stability Test Test sample: Olive leaf extract (OELE) herbal antibacterial skin gel of the present invention Testing basis: Appendix C of GB15979-2002 "Hygienic Standard for Disposable Sanitary Products".

[0046] Testing environment: temperature 22.1℃-22.9℃, relative humidity 53%-58%.

[0047] Storage conditions: Store in sealed, intact original packaging in a constant temperature and humidity chamber at 37°C and 75% relative humidity for 90 days.

[0048] result 1. Appearance like Figure 5 As shown, after the sample was placed in a constant temperature and humidity chamber at 37°C and 75% relative humidity for 90 days, the sample showed no color change, no suspended matter, and no sediment.

[0049] 2. Staphylococcus aureus stability test The experiment was repeated three times under constant temperature conditions of 20℃±1℃. The average inhibition rates against Staphylococcus aureus after 2 min, 5 min, 10 min, and 20 min were 43.27%, 52.20%, 54.20%, and 60.38%, respectively.

[0050] Table 2. Stability test of olive leaf extract (OELE) herbal antibacterial gel against Staphylococcus aureus. 3. Stability test of Candida albicans The experiment was repeated three times under constant temperature conditions of 20℃±1℃. The average inhibition rates against Candida albicans after 2 min, 5 min, 10 min, and 20 min were 40.50%, 51.25%, 53.44%, and 57.48%, respectively.

[0051] Table 3. Stability test of Olive Leaf Extract (OELE) Herbal Antibacterial Skin Gel against Candida albicans. 4. Stability test of Escherichia coli The experiment was repeated three times under constant temperature conditions of 20℃±1℃. The average inhibition rates against Escherichia coli after 2 min, 5 min, 10 min, and 20 min were 46.21%, 54.41%, 57.46%, and 63.18%, respectively (Table 4).

[0052] Table 4. Stability test of olive leaf extract (OELE) herbal antibacterial gel against Escherichia coli. Conclusion: After being placed in a constant temperature and humidity chamber at 37℃ and 75% relative humidity for 90 days, the sample showed no color change, no precipitation, and no suspended matter. After 5 minutes of action, the average inhibition rate against Candida albicans was 51.25%, against Staphylococcus aureus was 52.20%, and against Escherichia coli was 54.41%, which still meets the antibacterial effect requirements of the product. According to Appendix C6.2 of GB 15979-2002 "Hygienic Standard for Disposable Sanitary Products", the shelf life of this product can be set at 2 years.

[0053] IV. Sensory and physicochemical tests 1. Inspection basis: The inspection shall be conducted in accordance with the methods specified in GB15979-2002 "Hygienic Standard for Disposable Sanitary Products" and the 2002 edition of "Disinfection Technical Specifications" issued by the National Health Commission.

[0054] 2. Conclusion: Tests show that the present invention is uniform and fine, free of impurities and odor, and has a pH value of 6.45, which meets the requirements.

[0055] Although the present invention has been described in detail above with general descriptions, specific embodiments, and experiments, modifications or improvements can be made to it, which will be obvious to those skilled in the art. Therefore, all such modifications or improvements made without departing from the spirit of the present invention fall within the scope of protection claimed by the present invention.

Claims

1. A herbal antibacterial skin gel containing olive leaf extract, characterized in that, The components include the following mass percentages: Olive leaf extract 1%-10%; Menthol 0.1%-0.2%; Hydrogel matrix 2.6%-12.3%; pH adjuster, used to adjust the pH of the gel to 5.0-7.0; The remainder is water; The hydrogel matrix is ​​composed of carbomer, sodium hyaluronate and butylene glycol, wherein carbomer accounts for 0.5%-2.0% of the total mass of the gel, sodium hyaluronate accounts for 0.1%-0.3% of the total mass of the gel, and butylene glycol accounts for 2%-10% of the total mass of the gel.

2. The herbal antibacterial skin gel according to claim 1, characterized in that, The pH adjuster is triethanolamine.

3. The herbal antibacterial skin gel according to claim 1, characterized in that, The content of oleuropein in the olive leaf extract is not less than 50%.

4. A method for preparing the herbal antibacterial skin gel as described in claim 1, characterized in that, Includes the following steps: (1) Dissolve the prescribed amounts of olive leaf extract and menthol in a portion of water to form phase A; (2) Mix the prescribed amounts of butanediol, carbomer, and sodium hyaluronate to form phase B; (3) Under stirring conditions, phase A is added to phase B and mixed evenly; (4) Add a pH adjuster to adjust the pH of the mixture to 5.0-7.0 to obtain the antibacterial gel.

5. The method according to claim 4, characterized in that, Step (3) is carried out under vacuum stirring conditions.

6. The use of the herbal antibacterial skin gel as described in claim 1 in the preparation of products for bacterial or fungal infections of the skin surface.

7. The application according to claim 6, characterized in that, The bacteria or fungi are selected from one or more of Staphylococcus aureus, Escherichia coli, and Candida albicans.

8. The use of the herbal antibacterial skin gel as described in claim 1 in the preparation of products for relieving skin itching or allergies caused by mosquito bites.

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