Intelligent preservation equipment for microbial agent

By designing partition racks and a cold air control system inside the cryopreservation box, combined with insulation blocks and temperature sensors, the delivery of cold air to each preservation test tube can be independently controlled. This solves the problem of cold air consumption caused by frequent opening of the cryopreservation box, and improves the low-temperature preservation effect of the bacterial agent and the sustainability of outdoor work.

CN121553508APending Publication Date: 2026-02-24JIANGXI YIDI BIOTECHNOLOGY CO LTD
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Patent Information

Application Number
CN202511710028.9
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-11-20
Publication Date
2026-02-24

AI Technical Summary

Technical Problem

Frequent opening and closing of the cryopreservation box to retrieve and store test tubes leads to faster consumption of cold air, limiting the effective time for outdoor bacterial collection and preservation of bacterial agents.

Method used

A smart preservation device for microbial agents was designed. The device uses a partition rack to separate the hollow rack slots inside the cryopreservation box. The delivery of cold air to each preservation test tube is independently controlled by a cold air control valve and a smart control module. Combined with the design of head and tail insulation blocks, cold air loss is reduced. Temperature sensors and pressure balance valves on the rack are used to maintain the low temperature of the test tubes.

Benefits of technology

It significantly reduces the consumption of cooling air, improves the low-temperature preservation effect of bacterial agents, extends the time for outdoor work, and ensures the stable preservation of bacterial agents in test tubes under low-temperature conditions.

✦ Generated by Eureka AI based on patent content.

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Abstract

The invention relates to the field of microbial agent preservation boxes, in particular to intelligent preservation equipment for a microbial agent. According to the intelligent preservation equipment for the microbial agent, the interior of a cryopreservation box is divided into a plurality of independent hollowed-out frame grooves through separation frames, a box door is also divided into a plurality of independent hollowed-out door grooves, and a group of head heat preservation blocks, placement frames and tail heat preservation blocks are jointly arranged between each hollowed-out door groove and the corresponding hollowed-out frame groove; only one storage test tube in one placement rack is taken and placed independently each time, the consumption of cold air flow when the storage test tubes are taken and placed is greatly reduced, and after the placement racks are pulled outwards, a small amount of cold air flow is intermittently conveyed to the storage test tubes in the placement racks, so that the storage test tubes are kept in a low-temperature state; the low-temperature preservation effect of the fungicide in the strain collection process is improved; the technical problem that the cold air consumption speed in the cryopreservation box is increased due to the fact that the cryopreservation box is frequently opened for taking and placing the preservation test tubes is solved.
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Description

Technical Field

[0001] This invention relates to the field of microbial agent storage boxes, and more particularly to an intelligent storage device for microbial agents. Background Technology

[0002] Microbial inoculants have wide applications in agriculture, industry, and medicine. Outdoor collection of fresh microbial strains allows access to the most primitive microorganisms naturally occurring in nature. These microorganisms have functions such as helping plants absorb nutrients, resisting pests and diseases, and enhancing plant stress resistance, making them an important resource for the biopesticide industry. After collecting the microbial strains outdoors, it is only necessary to promptly mix the collected strains with culture reagents in preservation test tubes to form an inoculant, and then store the inoculant in a portable cryopreservation box. This allows the inoculant to be stored for a long time in a specified low-temperature environment. However, since the liquid refrigerant inside the portable cryopreservation box is limited, the cryopreservation box needs to be opened every time the inoculant is stored. The preservation test tubes containing the inoculant are placed into the cryopreservation box, and some of the cold air inside the cryopreservation box is injected into the cryopreservation box to cool it down to the specified temperature. Frequent opening and closing of the cryopreservation box will lead to a faster consumption of cold air, reducing the effective time for outdoor cryopreservation and limiting the duration of outdoor microbial strain collection and inoculant storage. Summary of the Invention

[0003] To overcome the drawback that frequent opening and closing of the cryopreservation box for storing test tubes will lead to faster consumption of cold air inside the cryopreservation box, this invention provides an intelligent preservation device for microbial agents.

[0004] Technical Solution: A smart microbial agent preservation device includes a cryopreservation box, a door, a partition rack, a cold air control valve, a smart control module, a placement rack, a tail insulation block, a head insulation block, and a U-shaped insert rod; the cryopreservation box is rotatably connected to the door; the cryopreservation box stores liquid refrigerant; a partition rack is fixedly connected inside the cryopreservation box; the partition rack has several hollowed-out rack slots; each area of ​​the cryopreservation box aligned with a hollowed-out rack slot is equipped with a cold air control valve to control the cold air flow; the cryopreservation box is equipped with a smart control module to control the cold air control valve; each hollowed-out rack slot of the partition rack... Each empty shelf slot contains a placement rack; the placement rack has several insertion slots for inserting insulated test tubes; a tail insulation block is fixed to the rear side of the placement rack; the door has perforated door slots corresponding to the number and position of the perforated shelf slots; each perforated door slot of the door contains a head insulation block; the head insulation block is in close contact with the corresponding placement rack; each head insulation block has a U-shaped insert rod slidably connected to it; the U-shaped insert rod has two buckles; the placement rack has two slot structures that correspond to the buckles; the interior of the freezer, the head insulation blocks, and the door is filled with foam insulation material.

[0005] Furthermore, each of the open slots in the divider is equipped with an internal temperature sensor.

[0006] Furthermore, the placement rack has a U-shaped air supply channel structure; the insertion slot of the placement rack has an air outlet structure that connects to the air supply channel; each cold air control valve is connected to the corresponding air supply channel of the placement rack by a telescopic air pipe, and the telescopic air pipe passes through the corresponding tail insulation block.

[0007] Furthermore, the air vent is designed with a downward-sloping structure.

[0008] Furthermore, a fixing plate is provided on one side of the freezer, and the fixing plate is provided with several insertion rings.

[0009] Furthermore, two C-shaped retaining strips are slidably connected in the insertion ring; each C-shaped retaining strip is fixedly connected to a compression spring between itself and the corresponding insertion ring.

[0010] Furthermore, an external temperature sensor is installed on the cryopreservation box.

[0011] Furthermore, the fixing plate is rotatably connected to the cryopreservation box via a rotating shaft, and the fixing plate is vertically attached to the cryopreservation box; the outer shell of the cryopreservation box is made of steel; and a permanent magnet of the cryopreservation box, made of magnetic steel material, is fixed to the surface of the fixing plate.

[0012] Furthermore, the left side of the cryopreservation box is rotatably connected to two parallel rotating arms; the right side of the cryopreservation box is also rotatably connected to two parallel rotating arms; a sunshade is rotatably connected between the four rotating arms to block sunlight outdoors.

[0013] Furthermore, the dividers are made of insulating foam material.

[0014] Furthermore, the cryopreservation box has a built-in pressure balancing valve that regulates the air pressure inside the perforated rack slots.

[0015] Beneficial Effects: The intelligent microbial agent preservation device of this invention has several independent hollowed-out rack slots separated by partitions inside the cryopreservation box, and several independent hollowed-out door slots on the box door. Each hollowed-out door slot and its corresponding hollowed-out rack slot are equipped with a set of head insulation blocks, a placement rack, and a tail insulation block. Only one preservation tube in the placement rack is taken out and placed at a time, which greatly reduces the amount of cold air consumed when taking out and placing the preservation tube. In addition, after the placement rack is pulled outward, a small amount of cold air can be intermittently delivered to the preservation tube in the placement rack, keeping the preservation tube at a low temperature and improving the low-temperature preservation effect of the microbial agent during the collection of microorganisms. It solves the technical problem that frequent opening of the cryopreservation box to take out and place preservation tubes will lead to a faster consumption of cold air in the cryopreservation box. Attached Figure Description

[0016] Figure 1 This is a structural diagram of an intelligent microbial inoculant preservation device.

[0017] Figure 2 This is a structural diagram of the cryopreservation box of an intelligent microbial agent preservation device.

[0018] Figure 3 This is a structural diagram of the partition rack of an intelligent microbial agent preservation device;

[0019] Figure 4 This is a structural diagram of the placement rack for an intelligent microbial inoculant preservation device.

[0020] Figure 5 This is a cross-sectional view of the placement rack and tail insulation block of an intelligent microbial agent preservation device.

[0021] Figure 6 This is a cross-sectional view of the cryopreservation box of an intelligent microbial agent preservation device.

[0022] Figure 7 This is a structural diagram of the door of an intelligent microbial inoculant preservation device.

[0023] Figure 8 This is a structural diagram of the head insulation block of an intelligent microbial agent preservation device.

[0024] Figure 9 This is a structural diagram of a U-shaped insert rod in an intelligent microbial agent preservation device.

[0025] Figure 10 This is a structural diagram of the fixed plate of an intelligent microbial agent preservation device.

[0026] Figure 11 This is a schematic diagram of the extended position of the placement rack of a smart microbial inoculant preservation device.

[0027] Attached reference numerals: 11-Freezer, 12-Door, 1201-Perforated door slot, 13-Divider rack, 1301-Perforated rack slot, 14-Cold air control valve, 15-Intelligent control module, 16-Internal temperature sensor, 17-External temperature sensor, 21-Placement rack, 2100-Card slot, 2101-Insertion slot, 2102-Gas delivery slot, 2103-Gas outlet, 22-Tail insulation block, 23-Head insulation block, 24-U-shaped insert rod, 2400-Snap fastener, 25-Telescopic air pipe, 31-Fixing plate, 32-Permanent magnet, 33-Insertion ring, 34-C-shaped clip, 35-Compression spring, 41-Rotating arm, 42-Sunshade. Detailed Implementation

[0028] The present invention will be further described below with reference to the accompanying drawings and embodiments.

[0029] Example 1: A smart preservation device for microbial agents, hereinafter referred to as the smart preservation device, such as... Figures 1-9 As shown, the system includes a freezer compartment 11, a door 12, a divider rack 13, a cold air control valve 14, an intelligent control module 15, a placement rack 21, a rear insulation block 22, a head insulation block 23, and a U-shaped insert rod 24. The freezer compartment 11 is rotatably connected to the door 12. A divider rack 13 is fixedly connected inside the freezer compartment 11. When the door 12 is directly flipped open forward, the head insulation block 23 flips forward with the door 12, moving away from the divider rack 13, exposing the entire divider rack 13 inside the freezer compartment 11 to the external environment. The divider rack 13 has several hollowed-out rack slots 1301. Each area of ​​the freezer compartment 11 aligned with each hollowed-out rack slot 1301 is equipped with a cold air control valve 14. The freezer compartment 11 is equipped with an intelligent control module 15 that controls the switching of each cold air control valve 14. A placement rack 21 is inserted into each hollowed-out rack slot 1301 of the divider rack 13. Each placement rack 21 has several insertion slots 2101. Structure: A preservation test tube is inserted into each insertion slot 2101; a tail insulation block 22 is fixed to the rear side of each placement rack 21; the box door 12 has perforated door slots 1201 corresponding to the number and position of the perforated rack slots 1301; a head insulation block 23 is inserted into each perforated door slot 1201 of the box door 12; the rear side of each head insulation block 23 is tightly attached to the corresponding placement rack 21, and the thickness of the head insulation block 23 in the front-to-back direction is the same as that of the box door 11. The thickness is the same in the front and back directions. The interior of the freezer 11, the head insulation block 23 and the door 12 are all filled with thick foam insulation material. Each head insulation block 23 is slidably connected with a U-shaped plug 24. Each U-shaped plug 24 is provided with two buckles 2400. Each shelf 21 has two slots 2100 structures that are adapted to the buckles 2400. The freezer 11 has a built-in air pressure balancing valve to adjust the air pressure inside the hollow shelf slot 1301.

[0030] like Figures 3-6As shown, the divider 13 uses thermal insulation foam material, which is lightweight, reducing the overall weight of the intelligent storage device. The freezer box 11 is 35-55cm high and has a small overall volume, making it easy to transport outdoors. The thermal insulation foam divider 13, together with the thermal insulation material inside the freezer box 11, the head insulation block 23, and the door 12, enhances the insulation effect of the intelligent storage device against external heat. Each open slot 1301 of the divider 13 is equipped with an internal temperature sensor 16, which can detect the temperature in the corresponding slot 1301. Each placement rack 21 has a U-shaped air duct 2102 structure. Each placement rack 21 has several air outlets 2103 on its insertion slot 2101 that connect to the air supply slot 2102; each cold air control valve 14 is connected to the corresponding air supply slot 2102 of the placement rack 21 by a telescopic air pipe 25, and the telescopic air pipe 25 passes through the corresponding tail insulation block 22; when the cold air control valve 14 outputs cold air outward, the cold air flows through the telescopic air pipe 25, the air supply slot 2102 of the placement rack 21 and the air outlets 2103 in sequence, and then is sprayed outward into the corresponding hollow rack slot 1301 in the separator rack 13; since the bacterial agent is accumulated in the area below the preservation test tube, each air outlet 2103 is designed to be inclined downward, so that the cold air is sprayed obliquely downward and preferentially covers the bacterial agent accumulation area below the preservation test tube.

[0031] The method of using the intelligent microbial agent preservation device of the present invention is as follows: Each internal temperature sensor 16 monitors the temperature in the corresponding hollowed-out rack slot 1301 of the partition rack 13, and the corresponding cold air control valve 14 controls the intermittent delivery of cold air to the hollowed-out rack slot 1301 to keep the temperature in the hollowed-out rack slot 1301 at a set low temperature. Outdoors, when the user needs to collect bacteria and preserve the agent, the user first pushes the U-shaped insert 24 on one of the head insulation blocks 23 to move it backward, so that the two buckles 2400 of the U-shaped insert 24 respectively engage in the two slots 2100 of the corresponding placement rack 21 on its rear side. Then, the user can pull the head insulation block 23 to pull the corresponding placement rack 21 out of the freezer 11. At the same time, the placement rack 21 pulls the corresponding tail insulation block 22 on its rear side forward to move into the corresponding hollowed-out door slot 1201 in the door 12. Figure 11As shown, the tail insulation block 22 replaces the head insulation block 23 to form a closed structure with the door 12, preventing the cold air in the cryopreservation box 11 from leaking out through the hollow door groove 1201. After the placement rack 21 and the preservation test tubes inserted inside are pulled out, the user can mix the collected bacterial cultures with the culture reagent to form a bacterial agent, and put the bacterial agent into different preservation test tubes for preservation. Finally, the user pushes the head insulation block 23 to push the placement rack 21 and the tail insulation block 22 back into the cryopreservation box 11 for cryopreservation.

[0032] After the user pulls out the rack 21 and the preservation tube, the corresponding cold air control valve 14 intermittently delivers cold air to the air supply channel 2102 of the rack 21. The cold air is sprayed obliquely downward through the air outlet 2103 into the lower part of the rack 21 and comes into contact with the bottom of the preservation tube. This intermittently sprayed small amount of cold air keeps the pulled-out preservation tube at a low temperature, ensuring that the bacterial agent stored in the preservation tube has a good low-temperature preservation effect during continuous bacterial collection.

[0033] Because the cryopreservation box 11 has a built-in pressure balancing valve to regulate the internal air pressure, each time the placement rack 21 and the tail insulation block 22 are pulled out or pushed back from the cryopreservation box 11, the pressure balancing valve adjusts the air pressure value inside the corresponding hollow rack slot 1301 structure, so that the air pressure value inside the preservation test tube on the placement rack 21 is kept within the normal range, ensuring that the bacterial agent inside the preservation test tube can be stored within the normal air pressure range.

[0034] Example 2, based on Example 1 above, as follows: Figures 1-10As shown, in this embodiment, a fixing plate 31 is rotatably connected to one side of the cryopreservation box 11 via a pivot. The fixing plate 31 is initially in a vertical position, tightly against the outer surface of the cryopreservation box 11, reducing the space occupied by the fixing plate 31 when not in use. During handling, the user's arms will not be obstructed by the fixing plate 31 while the user's arms are pressed against the side wall of the cryopreservation box 11 and both palms against the bottom of the cryopreservation box 11. The outer shell of the cryopreservation box 11 is made of ferromagnetic steel. A permanent magnet 32 ​​is fixedly attached to the surface of the fixing plate 31, and the fixing plate 31 is initially held vertically by the permanent magnet 32 ​​against the outer surface of the steel cryopreservation box 11. The fixing plate 31 has several insertion rings 33. Each insertion ring 33 has two symmetrically arranged C-shaped locking strips 34 slidably connected to it. Each C-shaped locking strip 34 is fixedly connected to a corresponding insertion ring 33 by a compression spring 35. An external temperature sensor 17 is installed on the storage box 11. When the user detects that the external environment is in a suitable temperature environment for bacterial growth through the external temperature sensor 17, the user first pulls the fixing plate 31 downwards and flips it 90 degrees to switch to a horizontal position. Then, the user pulls the head insulation block 23 to pull the placement rack 21 out of the cryopreservation box 11. After that, the preservation test tubes in the placement rack 21 are taken out and inserted between the two C-shaped clips 34 in each insertion ring 33. The inserted preservation test tubes push the C-shaped clips 34 to drive the compression spring 35 to compress. The compressed compression spring 35 pushes the C-shaped clips 34 to firmly clamp the preservation test tubes under the action of the reverse elastic force. Finally, the user pushes the head insulation block 23 to re-insert the placement rack 21 into the cryopreservation box 11 so that the placement rack 21 is no longer exposed to the external environment. At this time, after completing the bacterial collection work, the user can directly use the preservation test tubes on the fixing plate 31 to preserve the bacterial agent.

[0035] Example 3, based on Example 1 above, as follows: Figures 1-9 As shown, in this embodiment, the left side of the cryopreservation box 11 is rotatably connected to two parallel rotating arms 41; the right side of the cryopreservation box 11 is also rotatably connected to two parallel rotating arms 41; a sunshade 42 is rotatably connected between the four rotating arms 41. The sunshade 42 is initially located at the top of the cryopreservation box 11, blocking direct sunlight from the top of the cryopreservation box 11; when the user is outdoors conducting bacterial sampling and bacterial agent preservation work, before pulling the head insulation block 23 to pull the placement rack 21 out of the cryopreservation box 11, the user first pulls the sunshade 42 to flip it forward and upward, and at the same time the sunshade 42 drives the rotating arms 41 to flip forward and upward, as shown. Figure 11As shown, the sunshade 42 is moved to block the front and top of the cryopreservation box 11. After the user pulls the head insulation block 23 to pull the rack 21 out of the cryopreservation box 11, the sunshade 42 can provide a temporary small-scale sunshade effect for the rack 21 that has been pulled out, so as to prevent the sunlight from shining directly on the rack 21 and causing the temperature of the test tubes stored in the rack 21 to rise sharply.

[0036] The above description is merely a specific embodiment of the present invention, but the scope of protection of the present invention is not limited thereto. Any variations or substitutions that can be easily conceived by those skilled in the art within the technical scope disclosed in the present invention should be included within the scope of protection of the present invention. Therefore, the scope of protection of the present invention should be determined by the scope of the claims.

Claims

1. A smart preservation device for microbial agents, comprising a cryopreservation box (11); A door (12) is rotatably connected to the freezer (11); Its characteristics are, It also includes a partition (13), a cold air control valve (14), an intelligent control module (15), a placement rack (21), a tail insulation block (22), a head insulation block (23), and a U-shaped insert (24). The freezer (11) stores liquid refrigerant inside; a partition rack (13) is fixedly connected inside the freezer (11); the partition rack (13) has several hollow rack slots (1301); each area of ​​the freezer (11) aligned with a hollow rack slot (1301) is provided with a cold air control valve (14) for controlling the injection of cold air; the freezer (11) is provided with an intelligent control module (15) for controlling the cold air control valve (14); each hollow rack slot (1301) of the partition rack (13) is provided with a placement rack (21); the placement rack (21) has several insertion slots (2101) for inserting insulated test tubes; the rear side of the placement rack (21) is fixedly connected to... Tail insulation block (22); the door (12) is provided with a hollow door groove (1201) that corresponds to the number and position of the hollow frame groove (1301); a head insulation block (23) is inserted into each hollow door groove (1201) of the door (12); the head insulation block (23) is close to the corresponding placement rack (21); a U-shaped plug (24) is slidably connected to each head insulation block (23); two buckles (2400) are provided on the U-shaped plug (24); two slots (2100) structures that are compatible with the buckles (2400) are provided on the placement rack (21); the interior of the freezer (11), the head insulation block (23) and the door (12) are all filled with foam insulation material.

2. The intelligent preservation device for microbial agents according to claim 1, characterized in that, An internal temperature sensor (16) is installed in each of the slots (1301) of the divider (13).

3. The intelligent preservation device for microbial agents according to claim 1, characterized in that, The placement rack (21) has a U-shaped air supply channel (2102) structure; the insertion slot (2101) of the placement rack (21) has an air outlet (2103) structure that connects to the air supply channel (2102); each cold air control valve (14) and the corresponding air supply channel (2102) of the placement rack (21) are connected by a telescopic air pipe (25), and the telescopic air pipe (25) passes through the corresponding tail insulation block (22).

4. The intelligent preservation device for microbial agents according to claim 3, characterized in that, The vent (2103) is designed to be inclined downwards.

5. The intelligent preservation device for microbial agents according to claim 1, characterized in that, A fixing plate (31) is provided on one side of the freezer (11), and a number of insertion rings (33) are provided on the fixing plate (31).

6. The intelligent preservation device for microbial agents according to claim 5, characterized in that, Two C-shaped retaining strips (34) are slidably connected in the insert ring (33); each C-shaped retaining strip (34) is fixedly connected to a compression spring (35) between itself and the corresponding insert ring (33).

7. The intelligent preservation device for microbial agents according to claim 1, characterized in that, An external temperature sensor (17) is installed on the freezer (11).

8. The intelligent preservation device for microbial agents according to claim 7, characterized in that, The fixing plate (31) is rotatably connected to the cryopreservation box (11) via a rotating shaft, and the fixing plate (31) is in a vertical position and tightly attached to the cryopreservation box (11); the outer shell of the cryopreservation box (11) is made of steel; a permanent magnet (32) of the cryopreservation box (11) made of magnetic steel is fixed to the surface of the fixing plate (31).

9. The intelligent preservation device for microbial agents according to claim 1, characterized in that, The left side of the freezer (11) is rotatably connected to two parallel rotating arms (41); the right side of the freezer (11) is also rotatably connected to two parallel rotating arms (41); the four rotating arms (41) are rotatably connected to a sunshade (42) for blocking sunlight outdoors.

10. A smart preservation device for microbial agents according to any one of claims 1-9, characterized in that, The freezer (11) has a built-in pressure balancing valve that adjusts the internal pressure of the hollow rack slot (1301).