Fat filtering gauze and preparation method thereof
By preparing fat-filtering gauze using modified cotton fibers, the problem of incomplete oil removal during fat purification was solved, achieving efficient and safe fat purification and improving the volume retention rate and surgical efficiency of fat transplantation.
Patent Information
- Application Number
- CN202511972537.9
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-12-25
- Publication Date
- 2026-02-24
AI Technical Summary
In existing fat grafting techniques, fat purification methods cannot effectively remove unnecessary liquid components, resulting in low volume retention rates, the risk of fat particle contamination, low surgical efficiency, and increased complications from multiple surgeries.
A fat-filtering gauze was prepared using modified cotton fibers. Through acid treatment, silane coupling agent modification, polyacrylate layer formation, and fluorosilane treatment, combined with plasma etching, a porous structure was formed, which improved the oil adsorption efficiency and reduced the fat adhesion.
It significantly improves fat purity, reduces the risk of postoperative oil cyst formation, rapidly filters fat, reduces fat loss, ensures safety and sterility, and improves surgical efficiency.
Abstract
Description
Technical Field
[0001] This invention relates to the field of gauze technology, specifically to a fat-filtering gauze and its preparation method. Background Technology
[0002] Autologous fat grafting is widely used in facial rejuvenation and reconstructive surgery. The volume retention rate of transplanted fat has always been a key technical challenge. Currently, in the field of fat grafting, neither the addition of adipose-derived stem cells from a regenerative medicine perspective nor the addition of growth factors from the blood has achieved the ideal volume retention, and widespread clinical application requires extensive data validation. Clinically, overfilling corrections are often used to reserve volume, or multiple fat grafting surgeries are performed in hopes of achieving the desired transplant effect. These methods can lead to significant facial swelling after fat grafting, increased postoperative complications, and high surgical costs. Returning to traditional fat grafting, the purification method of the transplanted fat significantly affects the final volume retention.
[0003] In autologous fat grafting, crucial fat purification methods include static settling, centrifugation, filtration and washing, and the cotton pad method. Centrifugation is currently the most widely used, based on the gold standard proposed by Professor Coleman of New York University. This involves centrifuging the aspirated fat at 1200g for 3 minutes; the resulting product is known as Coleman fat. However, centrifugation cannot completely remove non-essential liquid components from the fat. Simple filtration and washing can be used for large-volume fat filtration, but it often needs to be combined with other methods like Telfa Rolling (cotton pad method) for fat concentration. The cotton pad method is an open fat processing method, increasing the risk of fat contamination; it is also time-consuming and has low surgical efficiency. In the cotton pad method, fat is poured directly onto the cotton pad, causing fat particles to adhere and resulting in additional waste. Ideal fat grafts should have low oil content, low non-essential liquid content, and a more intact tissue structure. This necessitates a simple and convenient approach to fat purification, removing unnecessary free oil droplets and liquid impurities to increase the effective fat content per unit volume. Simultaneously, it requires minimizing fat particle contamination, obtaining highly concentrated fat grafts, and improving postoperative fat volume retention. However, current fat purification and concentration methods do not adequately meet these requirements. Summary of the Invention
[0004] The purpose of this invention is to propose a fat-filtering gauze and its preparation method, which has good oil adsorption efficiency, does not easily adhere to fat, has little loss, reduces the risk of postoperative oil cyst formation, has a fast filtration speed, is free of bacteria and endotoxins, is safe and effective, and has broad application prospects.
[0005] The technical solution of this invention is implemented as follows:
[0006] This invention provides a method for preparing fat-filtering gauze. Cotton fibers are acid-treated, their surface is modified with silane coupling agent KH570, polyacrylate is polymerized on the surface, then fluorosilane surface treatment is performed, plasma etching is carried out, and then the fibers are interwoven to form gauze, thus obtaining fat-filtering gauze.
[0007] As a further improvement to the present invention, the following steps are included:
[0008] S1. The cotton fibers are immersed in an acid solution, filtered, washed, and dried to obtain pretreated cotton fibers;
[0009] S2. Add the pretreated cotton fibers to ethanol, add silane coupling agent KH570, heat and stir to react, filter, wash, and dry to obtain modified cotton fibers.
[0010] S3. Modified cotton fiber, methyl methacrylate, methyl acrylate, and butyl acrylate are added to acetonitrile. Under inert gas protection, an initiator is added, and the mixture is heated and stirred to react. The mixture is then filtered, washed, and dried to obtain polyacrylic acid modified cotton fiber.
[0011] S4. Polyacrylic acid modified cotton fiber is added to fluorosilane, heated, filtered, washed, and dried to obtain polyacrylic acid modified cotton fiber with surface fluorosilane treatment;
[0012] S5. The surface of polyacrylic acid modified cotton fibers treated with fluorosilane is plasma etched, then interwoven to form gauze, sterilized, and fat filter gauze is obtained.
[0013] As a further improvement of the present invention, the acid solution in step S1 is concentrated nitric acid, and the immersion time is 1-2 hours.
[0014] As a further improvement of the present invention, the mass ratio of the pretreated cotton fiber and the silane coupling agent KH570 in step S2 is 100:3-5.
[0015] As a further improvement of the present invention, the heating and stirring reaction in step S2 is carried out at a temperature of 45-55°C for 2-4 hours.
[0016] As a further improvement of the present invention, the mass ratio of the modified cotton fiber, methyl methacrylate, methyl acrylate, butyl acrylate and initiator in step S3 is 10-20:2-3:1-2:0.5-1:0.01-0.015.
[0017] As a further improvement of the present invention, the heating and stirring reaction in step S3 is carried out at a temperature of 50-60°C for 3-5 hours.
[0018] As a further improvement of the present invention, the mass ratio of the polyacrylic acid modified cotton fiber and the fluorosilane in step S4 is 1:5-10, the fluorosilane is perfluorooctyltriethoxysilane, and the temperature of the heat treatment is 60-80°C and the time is 1-2 hours.
[0019] As a further improvement of the present invention, the plasma etching in step S5 is performed using pure argon gas etching with a power of 100-200W, a gas flow rate of 50-150sccm, and a time of 60-90s.
[0020] The present invention further protects a fat-filtering gauze prepared by the above-described preparation method.
[0021] The present invention has the following beneficial effects:
[0022] The fat filtration gauze of this invention, with cotton fibers as its core, can effectively adsorb free lipid droplets (oil released from damaged fat cells), significantly improving the purity of transplanted fat and reducing the risk of postoperative oil cyst formation. Simultaneously, after acid treatment, the surface hydroxyl groups undergo a grafting reaction, reacting with a silane coupling agent containing double bonds to copolymerize with acrylic monomers, forming a lipophilic polyacrylate layer on its surface. This porous structure allows for rapid adsorption of free lipid droplets through capillary action and intermolecular forces. Furthermore, it encapsulates the cotton fibers, preventing lint from shedding during filtration and ensuring it doesn't mix into the transplanted fat.
[0023] Then, the polyacrylic acid modified cotton fiber is treated with fluorosilane, which makes its surface energy much lower than that of the cell membrane of fat cells, reducing the adhesion of intact cell clusters. In contrast to "lipophilicity", it makes fat clusters easy to transfer and collect, and will not stick tightly to the gauze and cause loss.
[0024] The fat-filtering gauze prepared by this invention has good oil adsorption efficiency, does not easily adhere to fat, has little loss, reduces the risk of postoperative oil cyst formation, and has a fast filtration speed. It is free of bacteria and endotoxins, safe and effective, and has broad application prospects. Detailed Implementation
[0025] The technical solutions in the embodiments of the present invention will be clearly and completely described below. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.
[0026] Example 1
[0027] This invention provides a method for preparing a fat-filtering gauze, comprising the following steps:
[0028] S1. The cotton fibers were immersed in concentrated nitric acid for 1 hour, filtered, washed, and dried to obtain pretreated cotton fibers.
[0029] S2. Add 10g of pretreated cotton fiber to 200mL of ethanol, add 0.3g of silane coupling agent KH570, heat to 45℃, stir and react for 2h, filter, wash, and dry to obtain modified cotton fiber.
[0030] S3. Add 10g of modified cotton fiber, 2g of methyl methacrylate, 1g of methyl acrylate, and 0.5g of butyl acrylate to 200mL of acetonitrile. Under nitrogen protection, add 0.01g of potassium persulfate, heat to 50℃, stir and react for 3h, filter, wash, and dry to obtain polyacrylic acid modified cotton fiber.
[0031] S4. Add 10g of polyacrylic acid modified cotton fiber to 50g of perfluorooctyltriethoxysilane, heat to 60℃, treat for 1h, filter, wash, and dry to obtain polyacrylic acid modified cotton fiber with surface fluorosilane treatment.
[0032] S5. The surface of the polyacrylic acid modified cotton fiber treated with fluorosilane is etched by pure argon plasma at a power of 100W, a gas flow rate of 50sccm, and a time of 60s. Then, the fibers are interwoven to form gauze, sterilized, and fat filter gauze is obtained.
[0033] Example 2
[0034] This invention provides a method for preparing a fat-filtering gauze, comprising the following steps:
[0035] S1. The cotton fibers were immersed in concentrated nitric acid for 2 hours, filtered, washed, and dried to obtain pretreated cotton fibers.
[0036] S2. Add 10g of pretreated cotton fiber to 200mL of ethanol, add 0.5g of silane coupling agent KH570, heat to 55℃, stir and react for 4h, filter, wash, and dry to obtain modified cotton fiber.
[0037] S3. Add 20g of modified cotton fiber, 3g of methyl methacrylate, 2g of methyl acrylate and 1g of butyl acrylate to 200mL of acetonitrile. Under nitrogen protection, add 0.015g of potassium persulfate, heat to 60℃, stir and react for 5h, filter, wash and dry to obtain polyacrylic acid modified cotton fiber.
[0038] S4. Add 10g of polyacrylic acid modified cotton fiber to 100g of perfluorooctyltriethoxysilane, heat to 80℃, treat for 2h, filter, wash, and dry to obtain polyacrylic acid modified cotton fiber with surface fluorosilane treatment.
[0039] S5. The surface of the polyacrylic acid modified cotton fiber treated with fluorosilane is etched by pure argon plasma at a power of 200W, a gas flow rate of 150sccm, and a time of 90s. Then, the fibers are interwoven to form gauze, sterilized, and fat filter gauze is obtained.
[0040] Example 3
[0041] This invention provides a method for preparing a fat-filtering gauze, comprising the following steps:
[0042] S1. The cotton fibers were immersed in concentrated nitric acid for 1.5 hours, filtered, washed, and dried to obtain pretreated cotton fibers.
[0043] S2. Add 10g of pretreated cotton fiber to 200mL of ethanol, add 0.4g of silane coupling agent KH570, heat to 50℃, stir and react for 3h, filter, wash, and dry to obtain modified cotton fiber.
[0044] S3. Add 15g of modified cotton fiber, 2.5g of methyl methacrylate, 1.5g of methyl acrylate and 0.7g of butyl acrylate to 200mL of acetonitrile. Under nitrogen protection, add 0.012g of potassium persulfate, heat to 55℃, stir and react for 4h, filter, wash and dry to obtain polyacrylic acid modified cotton fiber.
[0045] S4. Add 10g of polyacrylic acid modified cotton fiber to 75g of perfluorooctyltriethoxysilane, heat to 70℃, treat for 1.5h, filter, wash, and dry to obtain polyacrylic acid modified cotton fiber with surface fluorosilane treatment.
[0046] S5. The surface of the polyacrylic acid modified cotton fiber treated with fluorosilane is etched by pure argon plasma at a power of 150W, a gas flow rate of 100sccm, and a time of 75s. Then, the fibers are interwoven to form gauze, sterilized, and fat filter gauze is obtained.
[0047] Comparative Example 1
[0048] The difference from Example 3 is that steps S2 and S3 were not performed.
[0049] Includes the following steps:
[0050] S1. The cotton fibers were immersed in concentrated nitric acid for 1.5 hours, filtered, washed, and dried to obtain pretreated cotton fibers.
[0051] S2. Add 10g of pretreated cotton fiber to 75g of perfluorooctyltriethoxysilane, heat to 70℃, treat for 1.5h, filter, wash, and dry to obtain cotton fiber with surface fluorosilane treatment.
[0052] S3. The surface of the cotton fibers treated with fluorosilane is etched by pure argon plasma at a power of 150W, a gas flow rate of 100sccm, and a time of 75s. The fibers are then interwoven to form gauze, sterilized, and fat-filtering gauze is obtained.
[0053] Comparative Example 2
[0054] The difference from Example 3 is that step S4 was not performed.
[0055] Includes the following steps:
[0056] S1. The cotton fibers were immersed in concentrated nitric acid for 1.5 hours, filtered, washed, and dried to obtain pretreated cotton fibers.
[0057] S2. Add 10g of pretreated cotton fiber to 200mL of ethanol, add 0.4g of silane coupling agent KH570, heat to 50℃, stir and react for 3h, filter, wash, and dry to obtain modified cotton fiber.
[0058] S3. Add 15g of modified cotton fiber, 2.5g of methyl methacrylate, 1.5g of methyl acrylate and 0.7g of butyl acrylate to 200mL of acetonitrile. Under nitrogen protection, add 0.012g of potassium persulfate, heat to 55℃, stir and react for 4h, filter, wash and dry to obtain polyacrylic acid modified cotton fiber.
[0059] S4. Polyacrylic acid modified cotton fibers are etched by pure argon plasma at a power of 150W, a gas flow rate of 100sccm, and a time of 75s. The fibers are then interwoven to form gauze, sterilized, and fat-filtering gauze is obtained.
[0060] Comparative Example 3
[0061] The difference from Example 3 is that step S5 was not performed.
[0062] Includes the following steps:
[0063] S1. The cotton fibers were immersed in concentrated nitric acid for 1.5 hours, filtered, washed, and dried to obtain pretreated cotton fibers.
[0064] S2. Add 10g of pretreated cotton fiber to 200mL of ethanol, add 0.4g of silane coupling agent KH570, heat to 50℃, stir and react for 3h, filter, wash, and dry to obtain modified cotton fiber.
[0065] S3. Add 15g of modified cotton fiber, 2.5g of methyl methacrylate, 1.5g of methyl acrylate and 0.7g of butyl acrylate to 200mL of acetonitrile. Under nitrogen protection, add 0.012g of potassium persulfate, heat to 55℃, stir and react for 4h, filter, wash and dry to obtain polyacrylic acid modified cotton fiber.
[0066] S4. Add 10g of polyacrylic acid modified cotton fiber to 75g of perfluorooctyltriethoxysilane, heat to 70℃, treat for 1.5h, filter, wash, and dry to obtain polyacrylic acid modified cotton fiber with surface fluorosilane treatment, which is fat filter gauze.
[0067] Test Example 1
[0068] The oil contact angle of the surfaces of the fat-filtering gauze prepared in Examples 1-3 and Comparative Examples 1-3 was tested, and the results are shown in Table 1.
[0069] Table 1
[0070] Group Oil contact angle (°) Example 1 134 Example 2 135 Example 3 137 Comparative Example 1 131 Comparative Example 2 84 Comparative Example 3 122
[0071] As can be seen from the table above, the fat-filtering gauze prepared in Examples 1-3 of the present invention has good oleophobicity.
[0072] Test Example 2
[0073] The fat-filtering gauze prepared in Examples 1-3 and Comparative Examples 1-3 was dried and weighed as m0. It was then soaked in oil, and after saturation adsorption, it was taken out, drained of oil, and weighed as m1. The amount of oil adsorbed was calculated.
[0074] Oil adsorption capacity = (m1 - m0) / m0 × 100%
[0075] The results are shown in Table 2.
[0076] Table 2
[0077] Group Oil adsorption capacity (g / g) Example 1 24.5 Example 2 25.2 Example 3 26.3 Comparative Example 1 13.2 Comparative Example 2 23.1 Comparative Example 3 20.3
[0078] As can be seen from the table above, the fat-filtering gauze prepared in Examples 1-3 of the present invention has good oil absorption properties.
[0079] Test Example 3
[0080] The fat-filtering gauze prepared in Examples 1-3 and Comparative Examples 1-3 was tested for in vitro cytotoxicity according to the ISO 10993-5 standard test method. The results are shown in Table 3.
[0081] Table 3
[0082] Group Cytotoxicity (grade) Example 1 0 Example 2 0 Example 3 0 Comparative Example 1 0 Comparative Example 2 0 Comparative Example 3 0
[0083] As can be seen from the table above, the fat-filtering gauze prepared in Examples 1-3 of the present invention has no cytotoxicity.
[0084] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the protection scope of the present invention.
Claims
1. A method for preparing a fat-filtering gauze, characterized in that, After acid treatment, the surface of cotton fibers is modified with silane coupling agent KH570, and polyacrylate is polymerized on the surface. Then, the fibers are treated with fluorosilane, plasma etched, and then interwoven to form gauze, thus producing fat filter gauze.
2. The preparation method according to claim 1, characterized in that, Includes the following steps: S1. The cotton fibers are immersed in an acid solution, filtered, washed, and dried to obtain pretreated cotton fibers; S2. Add the pretreated cotton fibers to ethanol, add silane coupling agent KH570, heat and stir to react, filter, wash, and dry to obtain modified cotton fibers; S3. Modified cotton fiber, methyl methacrylate, methyl acrylate, and butyl acrylate are added to acetonitrile. Under inert gas protection, an initiator is added, and the mixture is heated and stirred to react. The mixture is then filtered, washed, and dried to obtain polyacrylic acid modified cotton fiber. S4. Polyacrylic acid modified cotton fibers are added to fluorosilane, heated, filtered, washed, and dried to obtain polyacrylic acid modified cotton fibers with fluorosilane surface treatment. S5. The surface of polyacrylic acid modified cotton fibers treated with fluorosilane is plasma etched, then interwoven to form gauze, sterilized, and fat filter gauze is obtained.
3. The preparation method according to claim 2, characterized in that, The acid solution mentioned in step S1 is concentrated nitric acid, and the immersion time is 1-2 hours.
4. The preparation method according to claim 2, characterized in that, The mass ratio of the pretreated cotton fiber and silane coupling agent KH570 in step S2 is 100:3-5.
5. The preparation method according to claim 2, characterized in that, The heating and stirring reaction in step S2 is carried out at a temperature of 45-55°C for 2-4 hours.
6. The preparation method according to claim 2, characterized in that, The mass ratio of the modified cotton fiber, methyl methacrylate, methyl acrylate, butyl acrylate, and initiator in step S3 is 10-20:2-3:1-2:0.5-1:0.01-0.
015.
7. The preparation method according to claim 2, characterized in that, The heating and stirring reaction in step S3 is carried out at a temperature of 50-60°C for 3-5 hours.
8. The preparation method according to claim 2, characterized in that, In step S4, the mass ratio of polyacrylic acid modified cotton fiber to fluorosilane is 1:5-10, the fluorosilane is perfluorooctyltriethoxysilane, and the heat treatment temperature is 60-80℃ for 1-2 hours.
9. The preparation method according to claim 2, characterized in that, The plasma etching described in step S5 uses pure argon gas etching with a power of 100-200W, a gas flow rate of 50-150sccm, and a time of 60-90s.
10. A fat-filtering gauze prepared by the method according to any one of claims 1-9.