Long-term culture system and culture method for abyssal macroorganisms

Abstract

The invention belongs to the technical field of abyssal macroorganism culture, and particularly discloses a abyssal macroorganism long-term culture system and method, and the abyssal macroorganism long-term culture system comprises a magnetic coupling type rodless seawater tank, a reaction kettle and an artificial seawater cabin. A magnetic coupling type rodless seawater cylinder comprises a cylinder body, a piston, an annular sliding block and a pressure sensor. The cylinder body is provided with a built-in cavity, and the piston is arranged in the built-in cavity in a sliding mode and divides the built-in cavity into a water inlet cavity and a water outlet cavity. The annular sliding block and the piston are magnetically coupled in a non-contact mode to form stable magnetic attraction force, the reaction kettle is provided with a water inlet end and a water outlet end, and the water inlet end, the first electromagnetic valve and the water inlet cavity are sequentially connected; the water outlet end, the second electromagnetic valve and the water outlet cavity are sequentially connected; the artificial seawater cabin, the booster pump, the third electromagnetic valve and the water inlet cavity are sequentially connected; the artificial seawater cabin, the booster pump, the fourth electromagnetic valve and the water outlet cavity are connected in sequence; according to the invention, isovolumetric replacement of new seawater and old seawater is realized, culture pressure is accurately stabilized, and biological physiological stress reaction can be effectively avoided.

Description

TECHNICAL FIELD

[0001] The present application belongs to the technical field of abyss macroorganism culture, and particularly relates to an abyss macroorganism long-term culture system and a culture method. BACKGROUND

[0002] Deep-sea scientific research has reached a new frontier, and the survival mechanism, metabolic capacity, etc. of organisms in the deep-sea ecosystem are important research directions. Deep-sea biological scientific research has reached a new frontier, and the deep-sea carbon cycle, extreme environmental adaptation mechanism of organisms, energy flow and biological metabolism are currently hot scientific issues. Due to the characteristics of high hydrostatic pressure, low or high temperature, insufficient light, nutrient deficiency and oxygen sparseness of deep-sea organisms, some organisms cannot survive in the process of returning to the sea surface after being captured due to the sharp changes in environmental factors such as pressure and marine inorganic content, so deep-sea organism culture equipment is needed to restore the extreme environmental conditions of the deep sea to meet the survival of deep-sea organisms.

[0003] The existing deep-sea organism culture equipment mainly uses a booster pump to directly inject high-pressure seawater into the culture kettle, but the local pressure pulse and temperature rise generated by the pump body operation (the booster process is accompanied by mechanical heat, causing the seawater temperature to rise by 10-20℃) destroy the stability of the in-situ low-temperature high-pressure environment. More importantly, the existing deep-sea organism culture equipment cannot achieve "equal volume matching of new seawater injection volume and old seawater discharge volume", either because the injection volume is greater than the discharge volume, causing a sharp rise in pressure, or because the discharge volume is excessive, causing a sharp drop in pressure, resulting in a large pressure fluctuation amplitude in the kettle, far exceeding the tolerance threshold of deep-sea organisms, easily causing physiological stress reactions such as abnormal cell membrane fluidity and enzyme activity disorder of deep-sea organisms, resulting in a generally low survival period of deep-sea organisms, making it difficult to support long-term metabolic mechanism research. SUMMARY

[0004] In view of the above problems, the present application aims to provide a deep-sea macroorganism long-term culture system and culture method.

[0005] The technical solution of the present application is: a deep-sea macroorganism long-term culture system, comprising a magnetic coupling type rodless seawater cylinder, a reaction kettle and an artificial seawater tank.

[0006] The magnetic coupling type rodless seawater cylinder comprises a cylinder body, a piston, an annular slider and a pressure sensor. The cylinder body is made of stainless steel and has an internal cavity in a columnar structure. The piston is made of magnetic material and is arranged in the internal cavity to separate the internal cavity into a water inlet cavity and a water outlet cavity. The annular slider is made of magnetic material and is arranged on the outside of the cylinder body in a sleeved manner. The annular slider is in non-contact magnetic coupling with the piston to form a stable magnetic attraction force. When the annular slider slides on the outside of the cylinder body, the magnetic field of the annular slider penetrates the side wall of the cylinder body to drive the adsorbed piston to slide in the internal cavity, so as to change the effective volume of the water inlet cavity and the water outlet cavity. The pressure sensor has two monitoring ends fixed on the cylinder body and located in the water inlet cavity and the water outlet cavity respectively. The two pressure sensors are used to monitor the pressure in the water inlet cavity and the water outlet cavity respectively.

[0007] The reaction kettle has a water inlet end and a water outlet end. The water inlet end, the first electromagnetic valve and the water inlet cavity are connected in sequence. The water outlet end, the second electromagnetic valve and the water outlet cavity are connected in sequence.

[0008] The artificial seawater tank is filled with artificial seawater. The artificial seawater tank, the booster pump, the third electromagnetic valve and the water inlet cavity are connected in sequence. The artificial seawater tank, the booster pump, the fourth electromagnetic valve and the water outlet cavity are connected in sequence. The artificial seawater in the artificial seawater tank is injected into the water inlet cavity and the water outlet cavity by the booster pump to increase the pressure. The effective volume of the water inlet cavity and the water outlet cavity is changed to realize the stable pressure and equal volume replacement of seawater in the reaction kettle.

[0009] Further, the magnetic coupling type rodless seawater cylinder has a plurality of water inlet cavities and a plurality of water outlet cavities. The water inlet cavities and the water outlet cavities of the plurality of magnetic coupling type rodless seawater cylinders are connected with the water inlet end and the water outlet end of the reaction kettle respectively. The plurality of water inlet cavities and the plurality of water outlet cavities are connected with the artificial seawater tank through the booster pump respectively.

[0010] Further, the cylinder body comprises a barrel and end caps. The barrel has a tubular structure with two open ends. The end caps have recess structures. The recess structures of the two end caps are correspondingly sleeved on the ends of the barrel to close the inside of the barrel and form the internal cavity in the barrel.

[0011] The piston is arranged in the barrel. The annular slider is sleeved on the barrel. The end caps are also used to limit the annular slider. The two pressure sensors are correspondingly fixed on the end caps.

[0012] Further, the barrel is embedded in the recess structure. The ends of the barrel embedded in the recess structure are provided with first through holes. The recess structure of the end cap is provided with second through holes corresponding to the positions of the first through holes and communicating with the first through holes. The first through holes and the second through holes form transmission holes. One of the transmission holes is used as a connection port of the water inlet cavity and is connected with the water inlet end of the reaction kettle and the artificial seawater tank respectively. The other transmission hole is used as a connection port of the water outlet cavity and is connected with the water outlet end of the reaction kettle and the artificial seawater tank respectively.

[0013] Further, the cylinder further comprises a slide rail base and a dovetail slide block. The slide rail base is parallel to the axis of the cylinder body and is fixed between the two end covers; the dovetail slide block is slidingly arranged on the slide rail base, and the annular slide block is fixed on the dovetail slide block.

[0014] Further, a constant temperature water bath is further included, and the magnetic coupling type rodless seawater cylinder and the reaction kettle are arranged in the constant temperature water bath.

[0015] Further, a plurality of sealing grooves are arranged on the side wall of the piston in contact with the built-in cavity, and a sealing strip is arranged in each sealing groove.

[0016] A deep-sea macroorganism long-term culture method, which utilizes the culture system to culture deep-sea macroorganisms, comprises the following steps: The first electromagnetic valve and the second electromagnetic valve are closed, the third electromagnetic valve and the fourth electromagnetic valve are opened, the artificial seawater tank injects seawater into the water inlet cavity and the water outlet cavity through the booster pump, and the water inlet cavity and the water outlet cavity are pressurized; The third electromagnetic valve and the fourth electromagnetic valve are closed, the first electromagnetic valve and the second electromagnetic valve are opened, the annular slide block drives the piston to slide, the effective volume of the water inlet cavity and the water outlet cavity is changed, and the seawater in the reaction kettle is isovolumetrically replaced.

[0017] Further, the ratio of the amount of seawater injected by the artificial seawater tank into the water inlet cavity and the water outlet cavity through the booster pump is (90-95):(5-10).

[0018] Compared with the prior art, the beneficial effects of the present application are that the present application avoids the pressure pulse and temperature rise problems of the traditional booster pump through magnetic coupling rodless transmission and double-cavity pressure monitoring, realizes isovolumetric replacement of new and old seawater, accurately and stably cultures the pressure, avoids biological physiological stress reaction, greatly prolongs the survival period of deep-sea macroorganisms, and provides reliable support for long-term scientific research.

[0019] The magnetic coupling type rodless seawater cylinder adopts a magnetic coupling non-contact transmission mode, and a stable magnetic attraction force is formed between the piston and the annular slider of the magnetic material. When the annular slider slides outside the cylinder body, it can penetrate the side wall of the stainless steel cylinder body to drive the piston to move smoothly, avoiding the local pressure pulse generated by the traditional direct transmission mode of the booster pump, and eliminating the pressure fluctuation that may be caused by mechanical contact transmission, thereby effectively maintaining the pressure stability of the culture environment. Two pressure sensors are arranged to monitor the pressures of the water inlet cavity and the water outlet cavity respectively, and provide data support for accurate regulation and control. The piston is isolated from the built-in cavity, and the effective volume of the water inlet cavity and the water outlet cavity is synchronously and reversely adjusted under the driving of the annular slider, so that the injection amount of new seawater and the discharge amount of old seawater are strictly matched, and the problem of sudden pressure rise and fall caused by the mismatch of the existing equipment is solved. The pressure fluctuation amplitude is controlled within the tolerance threshold of the hadal organism, and physiological stress reactions such as abnormal cell membrane fluidity and enzyme activity disorder are avoided.

[0020] In addition, through the cooperation of the electromagnetic valve and the booster pump, the precise on-off and pressure boosting control of seawater delivery are realized, the artificial seawater tank can stably supply seawater suitable for the deep-sea environment, and the continuous stability of the culture system can be ensured without additional complex regulation and control, which greatly improves the survival period of the deep-sea macroorganism, provides reliable equipment support for long-term scientific research on the survival mechanism and metabolic capacity of the deep-sea organism, and at the same time, the selection of the stainless steel cylinder body and the magnetic components takes into account the pressure resistance and transmission stability of the equipment, prolongs the service life of the equipment, and has good practicality and popularization value. BRIEF DESCRIPTION OF DRAWINGS

[0021] Figure 1 is a structural schematic view of embodiment 1 of the present application; Figure 2 is a structural schematic view of embodiment 1 of the present application; Figure 3 is a front view of the magnetic coupling type rodless seawater cylinder of the present application; Figure 4 is a front view of the magnetic coupling type rodless seawater cylinder of the present application; Figure 5 is a structural schematic view of embodiment 2 of the present application.

[0022] 1-magnetic coupling type rodless seawater cylinder, 10-built-in cavity, 101-water inlet cavity, 102-water outlet cavity, 11-cylinder body, 111-cylinder body, 112-end cover, 113-sliding rail base, 114-dovetail slider, 12-piston, 120-sealing strip, 13-annular slider, 14-pressure sensor, 2-reaction kettle, 3-artificial seawater tank, 30-booster pump, 4-constant temperature water bath box. DETAILED DESCRIPTION

[0023] The following will be described in combination with Figures 1 to 5The specific embodiments of the present invention will be described in detail below. In the description of the present invention, it should be understood that the terms "center," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," and "outer," etc., indicating orientations or positional relationships, are based on the orientations or positional relationships shown in the accompanying drawings and are only for the convenience of describing the present invention and simplifying the description, and are not intended to indicate or imply that the device or element referred to must have a specific orientation or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of the present invention.

[0024] The terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature; in the description of this invention, unless otherwise stated, "a plurality of" means two or more.

[0025] It should be noted that the circuit connections involved in this invention all adopt conventional circuit connection methods and do not involve any innovation.

[0026] Example 1 like Figure 1 The system shown is a long-term culture system for deep-sea macroorganisms, including a magnetically coupled rodless seawater tank 1, a reaction vessel 2, and an artificial seawater chamber 3.

[0027] like Figure 2 As shown, the magnetically coupled rodless seawater tank 1 includes a cylinder body 11, a piston 12, an annular slider 13, and pressure sensors 14. The cylinder body 11 is made of stainless steel and has an internal cavity 10, which is a cylindrical structure. The piston 12 is made of magnetic material and is slidably disposed within the internal cavity 10, dividing the internal cavity 10 into an inlet cavity 101 and an outlet cavity 102. The annular slider 13 is made of magnetic material and is slidably sleeved on the outside of the cylinder body 11. The annular slider 13 and the piston 12 form a stable magnetic attraction force through non-contact magnetic coupling. When the annular slider 13 slides on the outside of the cylinder body 11, the magnetic field of the annular slider 13 penetrates the side wall of the cylinder body 11, causing the adsorption piston 12 to slide within the internal cavity 10, thereby changing the effective volume of the inlet cavity 101 and the outlet cavity 102. There are two pressure sensors 14, which are fixed on the cylinder body 11, with their monitoring ends located inside the inlet cavity 101 and the outlet cavity 102, respectively. The two pressure sensors 14 are used to monitor the pressure inside the inlet cavity 101 and the outlet cavity 102, respectively. It should be noted that both piston 12 and annular slider 13 are made of samarium cobalt permanent magnets.

[0028] The reactor 2 has an inlet end and an outlet end; the inlet end, the first solenoid valve, and the inlet chamber 101 are connected in sequence; the outlet end, the second solenoid valve, and the outlet chamber 102 are connected in sequence.

[0029] The artificial seawater tank 3 is internally placed with artificial seawater, the artificial seawater tank 3, the booster pump 30, the third electromagnetic valve, and the water inlet cavity 101 are sequentially connected; the artificial seawater tank 3, the booster pump 30, the fourth electromagnetic valve, and the water outlet cavity 102 are sequentially connected; the booster pump 30 is used to inject the artificial seawater in the artificial seawater tank 3 into the water inlet cavity 101 and the water outlet cavity 102 and to boost the pressure, and the effective volumes of the water inlet cavity 101 and the water outlet cavity 102 are changed to realize the constant pressure and equal volume replacement of seawater in the reaction kettle 2.

[0030] Preferably, the plurality of magnetic coupling type rodless seawater cylinders 1 are connected with the water inlet end and the water outlet end of the reaction kettle 2 through the water inlet cavity 101 and the water outlet cavity 102 respectively, and the plurality of water inlet cavities 101 and the plurality of water outlet cavities 102 are connected with the artificial seawater tank 3 through the booster pump 30 respectively.

[0031] Preferably, as shown in Figure 2 、 Figure 3 、 Figure 4 , the cylinder body 11 includes a barrel body 111 and end covers 112, the barrel body 111 is a cylindrical structure with both ends being open, and the end covers 112 are two, the two end covers 112 have groove structures, and the groove structures of the two end covers 112 are correspondingly sleeved on the ends of the barrel body 111 to close the inside of the barrel body 111 and form the built-in cavity 10 in the inside of the barrel body 111.

[0032] The piston 12 is slidingly arranged in the barrel body 111, the annular sliding block 13 is sleeved on the barrel body 111, the end covers 112 are further used for limiting the annular sliding block 13, and the two pressure sensors 14 are correspondingly fixed on the end covers 112.

[0033] Preferably, the first through holes are arranged at the ends of the barrel body 111 embedded in the grooves, the second through holes corresponding to the positions of the first through holes and communicating with the first through holes are arranged on the groove structures of the end covers 112, the first through holes and the second through holes form transmission holes, one of the transmission holes is used as the connection port of the water inlet cavity 101 and is connected with the water inlet end of the reaction kettle 2 and the artificial seawater tank 3 respectively, and the other transmission hole is used as the connection port of the water outlet cavity 102 and is connected with the water outlet end of the reaction kettle 2 and the artificial seawater tank 3 respectively.

[0034] Preferably, as shown in Figure 3 、 Figure 4 , the cylinder body 11 further includes a sliding rail base 113 and a dovetail sliding block 114. The sliding rail base 113 is parallel to the axis of the barrel body 111 and is fixed between the two end covers 112, the dovetail sliding block 114 is slidingly arranged on the sliding rail base 113, and the annular sliding block 13 is fixed on the dovetail sliding block 114.

[0035] It should be noted that: swallow tail slider 114 as the power source of the ring slider 13 to drive the ring slider 13 sliding, and the swallow tail slider 114 is realized by the external driving member to slide on the slide rail base 113, the external driving member can adopt the market electric push rod.

[0036] Preferably, as shown in the figure, Figure 2 The piston 12 is provided with a plurality of sealing grooves on the side wall in contact with the built-in cavity 10, and a sealing strip 120 is placed in each sealing groove.

[0037] A deep-sea macroorganism long-term culture method, using the culture system proposed in this embodiment to culture deep-sea macroorganisms, includes the following steps: Close the first electromagnetic valve and the second electromagnetic valve, open the third electromagnetic valve and the fourth electromagnetic valve, and the artificial seawater tank 3 injects seawater into the water inlet cavity and the water outlet cavity through the booster pump 30 to realize the pressure boosting of the water inlet cavity and the water outlet cavity. Specifically, first, use the booster pump 30 to inject 5%~10% of the total volume of the built-in cavity 10 into the water outlet cavity at normal pressure, and then continuously inject seawater into the water inlet cavity until the pressure is increased to 30MPa rated pressure.

[0038] Close the third electromagnetic valve and the fourth electromagnetic valve, open the first electromagnetic valve and the second electromagnetic valve, use the ring slider 13 to drive the piston 12 to slide, change the effective volume of the water inlet cavity 101 and the water outlet cavity 102, and realize the constant volume replacement of the seawater in the reaction kettle 2.

[0039] It should be noted that: as shown in the figure, Figure 1 The embodiment has four, so the four magnetic coupling type rodless seawater cylinders 1 are used in turn, when a magnetic coupling type rodless seawater cylinder 1 injects seawater into the reaction kettle 2, the remaining magnetic coupling type rodless seawater cylinders 1 work to inject seawater into the built-in cavity 10, to ensure that the four magnetic coupling type rodless seawater cylinders 1 and the reaction kettle 2 inside continuously realize the constant volume replacement.

[0040] Example 2 Different from example 1: preferably, as shown in the figure, Figure 5 The culture system further includes a constant temperature water bath box 4, and the magnetic coupling type rodless seawater cylinder 1 and the reaction kettle 2 are arranged in the constant temperature water bath box 4.

[0041] It should be noted that: the constant temperature water bath box 4 of this embodiment adopts a commercially available 380V circulating experimental water bath box, and in actual use, the magnetic coupling type rodless seawater cylinder 1 and the reaction kettle 2 need to be placed in the water bath box for long-term soaking, so that the inner wall of the magnetic coupling type rodless seawater cylinder 1 and the reaction kettle 2 reaches the target temperature condition.

[0042] The specific models of the electronic components are not specially specified, and common products on the market can be selected as long as the use requirements of the present application are met.

[0043] The above-described specific embodiments further illustrate the purpose, technical solutions and beneficial effects of the present application, and it should be understood that the above-described are only specific embodiments of the present application and do not limit the present application. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present application is included in the protection scope of the present application.

Claims

1. A deep-sea macroorganism long-term culture system, characterized by comprising: include: A magnetically coupled rodless seawater tank includes: a tank body made of stainless steel, having an internal cavity, wherein the internal cavity is a cylindrical structure; The piston, made of magnetic material, is slidably positioned within the internal cavity, dividing it into an inlet and an outlet chamber. The annular slider, also made of magnetic material, is slidably fitted onto the outside of the cylinder. The annular slider and piston are magnetically coupled non-contactly, forming a stable magnetic attraction. When the annular slider slides on the outside of the cylinder, its magnetic field penetrates the sidewall of the cylinder, causing the adsorbed piston to slide within the internal cavity, thus changing the effective volume of the inlet and outlet chambers. Two pressure sensors are fixed to the cylinder, with their monitoring ends located inside the inlet and outlet chambers, respectively. These two pressure sensors are used to monitor the pressure inside the inlet and outlet chambers. The reactor has an inlet and an outlet; the inlet, a first solenoid valve, and an inlet chamber are connected in sequence; the outlet, a second solenoid valve, and an outlet chamber are connected in sequence. The artificial seawater chamber contains artificial seawater. The artificial seawater chamber, booster pump, third solenoid valve, and inlet chamber are connected in sequence. The artificial seawater chamber, booster pump, fourth solenoid valve, and outlet chamber are connected in sequence. The booster pump injects the artificial seawater from the artificial seawater chamber into the inlet and outlet chambers and pressurizes it. By changing the effective volume of the inlet and outlet chambers, the pressure of the seawater inside the reactor is stabilized and the volume is replaced.

2. The abyssal macroorganism long-term culture system according to claim 1, wherein There are multiple magnetically coupled rodless seawater tanks, and the inlet and outlet chambers of the multiple magnetically coupled rodless seawater tanks are connected to the inlet and outlet ends of the reaction vessel, respectively; the multiple inlet and outlet chambers are connected to the artificial seawater chamber through a booster pump.

3. The abyssal macroorganism long-term culture system according to claim 1, wherein The cylinder body includes: The cylindrical body is a cylindrical structure open at both ends; There are two end caps, each with a grooved structure. The grooved structures of the two end caps are fitted one-to-one onto the ends of the cylinder to seal the inside of the cylinder and form the internal cavity inside the cylinder. The piston is slidably disposed inside the cylinder, the annular slider is sleeved on the cylinder, the end cap is also used to limit the annular slider, and two pressure sensors are fixed on the end cap one to one.

4. The abyssal macroorganism long-term culture system according to claim 3, wherein The cylindrical body is embedded in the groove structure at both ends, and a first through hole is provided at each end. The groove structure of the end cap is provided with a second through hole that corresponds to and communicates with the first through hole. The first through hole and the second through hole constitute a transmission hole. One of the transmission holes serves as the connection port of the water inlet chamber and is connected to the water inlet end of the reactor and the artificial seawater tank respectively. The other transmission hole serves as the connection port of the water outlet chamber and is connected to the water outlet end of the reactor and the artificial seawater tank respectively.

5. The abyssal macroorganism long-term culture system according to claim 3, wherein The cylinder block also includes: The slide rail base is parallel to the axis of the cylinder and fixed between the two end caps; The dovetail slider is slidably mounted on the slide rail base, and the annular slider is fixed on the dovetail slider.

6. The long-term culture system for deep-sea macroorganisms as described in claim 1, characterized in that, It also includes a constant temperature water bath, in which the magnetically coupled rodless seawater tank and the reaction vessel are arranged.

7. The long-term culture system for deep-sea macroorganisms as described in claim 1, characterized in that, The piston has multiple sealing grooves on the side wall that contacts the internal cavity, and a sealing strip is placed in each sealing groove.

8. A method for long-term cultivation of deep-sea macroorganisms, characterized in that, The cultivation of abyssal macroorganisms using the culture system described in any one of claims 1-7 includes the following steps: When the first and second solenoid valves are closed and the third and fourth solenoid valves are opened, the artificial seawater chamber injects seawater into the inlet and outlet chambers through the booster pump, thereby pressurizing the inlet and outlet chambers. Close the third and fourth solenoid valves, open the first and second solenoid valves, and use the annular slider to drive the piston to slide, changing the effective volume of the inlet and outlet water chambers, thereby achieving pressure stabilization and volume replacement of the seawater inside the reactor.

9. The long-term culture method for deep-sea macroorganisms as described in claim 8, characterized in that, The ratio of the amount of seawater injected into the inlet chamber and the outlet chamber by the booster pump in the artificial seawater chamber is (90~95):(5~10).

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