Diglyceride composite edible oil for assisting in reducing blood sugar and blood fat and preparation method of diglyceride composite edible oil

Abstract

The invention discloses diglyceride composite edible oil assisting in reducing blood sugar and blood fat and a preparation method thereof, and relates to the technical field of functional edible oil. The diglyceride composite edible oil for assisting in reducing blood sugar and blood fat is prepared from the following raw materials in parts by weight: 40 to 60 parts of high-purity diglyceride oil, 2 to 3 parts of modified flaxseed oil micro powder, 2 to 3 parts of modified camellia oleosa seed oil microcapsule powder, 5 to 8 parts of perilla seed oil, 2 to 5 parts of phytosterol ester, 1 to 3 parts of resistant dextrin, 0.1 to 0.5 part of rosemary extract and 0.05 to 0.15 part of potassium citrate. The compound edible oil is scientific in formula, and the high-purity diglyceride oil is used as a core and matched with various functional components such as the modified flaxseed oil micro powder to synergistically play a role in assisting in reducing blood sugar and blood fat. Through an exclusive modification process, gradient high-pressure homogenization and other standardized preparation processes, the stability and dispersion uniformity of the components are improved, the absorption rate of the active components is high, the storage life is long, no artificially synthesized additive is added, the food is safe to eat, and practicability and health are both considered.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of functional edible oil, in particular to a glyceride composite edible oil for assisting in reducing blood sugar and blood lipid and a preparation method thereof. BACKGROUND

[0002] With the change of people's lifestyle and dietary structure, the excessive intake of high-calorie, high-fat and high-sugar food leads to the increasing incidence of metabolic diseases such as diabetes and hyperlipidemia, and the trend of younger generation, which has become an important public health problem affecting public health. This kind of disease not only can cause multiple organ complications such as cardiovascular and kidney, but also can significantly reduce the quality of life of patients, therefore, developing food with daily edible properties and auxiliary metabolic function has become a research hotspot in the industry.

[0003] As an indispensable component in daily diet, the nutritional value and functionality of edible oil are concerned. Traditional edible oil is mainly composed of triglycerides, which is easy to cause fat accumulation and aggravate blood lipid abnormalities and blood sugar fluctuations if consumed in excess for a long time. Moreover, the existing ordinary edible oil lacks specific auxiliary regulation effect. Although some functional oil products have tried to add unsaturated fatty acids, phytosterols and other ingredients, there are still many technical bottlenecks: first, the functional ingredients are single, and the components lack synergistic effect, so the effect of reducing blood sugar and blood lipid is limited; second, the natural active ingredients are easy to be oxidized and deteriorated by temperature and oxygen during processing and storage, and the stability is insufficient; third, the compatibility of oil and functional powder ingredients is poor, and the dispersion uniformity is poor, which is easy to cause stratification and precipitation, affecting the sensory and use experience of the product; fourth, the absorption rate of functional ingredients is low, and some ingredients are easy to be destroyed by gastric acid, which is difficult to be effectively absorbed in the intestine.

[0004] In addition, the preparation process of the existing functional edible oil lacks standardized design, the selection and control of raw materials are not strict, and the process parameters fluctuate greatly, resulting in poor consistency of product quality. Moreover, some products add artificial synthetic antioxidants, stabilizers and other additives, which have potential food safety hazards. Therefore, it is necessary to develop a composite edible oil for assisting in reducing blood sugar and blood lipid, which has scientific formula, synergistic components, strong stability, high absorption rate of functional ingredients and standardized preparation process, so as to meet the needs of consumers for healthy diet and fill the technical gap of existing products, which has important market value and application prospect. SUMMARY

[0005] In view of the deficiencies of the prior art, the present application provides a glyceride composite edible oil for assisting in reducing blood sugar and blood lipid and a preparation method thereof, which solves the problems of limited effect of existing edible oil in reducing blood sugar and blood lipid, poor stability of functional ingredients, insufficient dispersion uniformity and low absorption rate of functional ingredients.

[0006] To achieve the above purpose, the technical scheme is as follows: A compound edible oil containing diglycerides that helps lower blood sugar and blood lipids comprises the following ingredients by weight: 40-60 parts high-purity diglyceride oil, 2-3 parts modified flaxseed oil micro powder, 2-3 parts modified camellia seed oil microcapsule powder, 5-8 parts perilla seed oil, 2-5 parts phytosterol esters, 1-3 parts resistant dextrin, 0.1-0.5 parts rosemary extract, and 0.05-0.15 parts potassium citrate.

[0007] Furthermore, the purity of the high-purity diglyceride oil is ≥90%. High purity ensures the functional activity of diglycerides, reduces the interference of impurities such as triglycerides on the metabolic regulation effect, and fully exerts its core role in reducing fat accumulation and optimizing blood lipid metabolism, providing a basic guarantee for overall efficacy.

[0008] Furthermore, the modified flaxseed oil powder is prepared using the following specific steps: A1. Flaxseed oil was washed with hot water at 80℃ for 30 min to remove anti-nutritional factors such as cyanogenic glycosides. After centrifugation, it was vacuum dried. The treated flaxseed oil was mixed with anhydrous ethanol and phosphate buffer at pH 6. Immobilized Rhizopus oryzae lipase was added, and the mixture was stirred at 300-400 r / min for 4 h at 45-50℃ and ultrasonic power of 200-300 W. Citric acid was then added to the system, the temperature was raised to 55℃, and 30% hydrogen peroxide solution was slowly added dropwise. The mixture was stirred at 200-250 r / min for 2 h. Citric acid and hydrogen peroxide synergistically oxidized and modified the oil structure. After the reaction, the mixture was centrifuged at 4000 r / min for 10 min, washed with water, and then distilled under reduced pressure at -0.09 MPa and 60℃ to remove the solvent, ensuring the safety and functional activity of the oil, thus obtaining the first modified flaxseed oil. A2. The first-modified flaxseed oil was mixed with β-cyclodextrin and deionized water, heated to 70℃, and stirred at 250-300 r / min for 1.5 h. The β-cyclodextrin encapsulated and protected the active ingredients from the influence of the external environment. After the reaction, the mixture was cooled to room temperature, centrifuged at 4000 r / min for 10 min, and then freeze-dried at -0.1 MPa and -40℃ to prevent oxidative degradation of the components. After pulverization, the second-modified flaxseed oil powder was obtained, which increased the contact area with digestive enzymes and laid the foundation for subsequent fermentation modification. A3. Mix the second modified flaxseed oil powder, glucose, and probiotic liquid, and let it ferment at 35℃, normal pressure, and nitrogen protection for 12 hours. After fermentation, add maltodextrin to the system as a carrier to enhance the stability of the micro powder. Dry it in a spray dryer with the inlet air temperature set at 120℃ and the outlet air temperature at 60℃. Low-temperature spray drying retains live bacteria and active ingredients, and finally obtains modified flaxseed oil micro powder.

[0009] Furthermore, the ratio of flaxseed oil, anhydrous ethanol, phosphate buffer, immobilized Rhizopus oryzae lipase, citric acid, and hydrogen peroxide solution in A1 is 100g:30-40ml:50-60ml:5-8g:10-15g:5-8ml; the immobilized Rhizopus oryzae lipase activity is 240-250U / g.

[0010] Furthermore, the ratio of the first modified flaxseed oil, β-cyclodextrin, and deionized water in A2 is 100g: 15-20g: 80-100ml.

[0011] Furthermore, in A3, the ratio of the second modified flaxseed oil powder, glucose, probiotic solution, and maltodextrin is 100g: 5-8g: 60-70ml: 10-15g; the probiotic solution is a mixed solution of Lactobacillus acidophilus and Lactobacillus plantarum, with a viable count ≥1.2×10⁻⁶. 9 CFU / ml.

[0012] Furthermore, the modified camellia seed oil microcapsule powder is prepared using the following specific steps: B1. Camellia seed oil was subjected to molecular distillation at a vacuum of -0.095 MPa and a temperature of 180℃ to remove tea saponins. The treated camellia seed oil was mixed with a 70% (v / v) ethanol solution and homogenized twice in a high-pressure homogenizer at a pressure of 40-50 MPa for 5 min each time. Immobilized Rhizopus oryzae lipase and acetic anhydride were added to the homogenized solution and stirred at 250-300 r / min for 3 h at 40℃. Sodium carbonate was added to neutralize to pH 7.0. Ethanol was removed by vacuum distillation and then centrifuged at 3000 r / min for 15 min to obtain the first modified camellia seed oil. B2. The first-modified camellia seed oil was mixed with glucose, and 0.1 mol / L hydrochloric acid was added to adjust the pH to 3.0. Under ultraviolet light with a wavelength of 254 nm, a power of 30 W, and a distance of 20 cm, the mixture was stirred at 200-250 r / min for 2 h. The ultraviolet irradiation promoted the reaction between the oil and glucose. 25% glutaraldehyde was added to the system, and the pH was adjusted to 8.0 with 0.5 mol / L sodium hydroxide. The temperature was raised to 50 °C, and the mixture was stirred at 300-350 r / min for 1.5 h. After the reaction, the mixture was washed three times with deionized water and dried under reduced pressure at -0.08 MPa and 50 °C. Reduced pressure drying prevented the oxidation of components and improved the structural stability and functional durability of the camellia seed oil, thus obtaining the second-modified camellia seed oil. B3. Octenyl succinate starch ester and Tween 80 were dissolved in deionized water and stirred until homogeneous to obtain an aqueous phase. The second modified camellia seed oil was used as the oil phase and slowly added to the aqueous phase. High-speed shearing at 10000 r / min for 10 min was performed to obtain a primary emulsion. The primary emulsion was placed in a high-pressure microjet mill and homogenized three times under a pressure of 80-100 MPa to form an ultrafine emulsion. High-speed shearing and high-pressure microjet synergistically achieved nanoscale emulsification and improved dispersion uniformity. The ultrafine emulsion was spray-dried with an inlet air temperature of 170℃ and an outlet air temperature of 75℃ to form a microcapsule structure and protect the active ingredients. The spray-dried powder was then placed in an air jet mill and depolymerized under a pressure of 0.6 MPa for 5 min to avoid particle agglomeration. The secondary particle size D50 was controlled to be 0.5-1 μm to increase the contact area with digestive enzymes and obtain modified camellia seed oil microcapsule powder.

[0013] Furthermore, the ratio of camellia seed oil, ethanol solution, immobilized Rhizopus oryzae lipase, and acetic anhydride in B1 is 100g:100-120ml:4-6g:8-10ml; the immobilized Rhizopus oryzae lipase has an activity of 240-250U / g.

[0014] Furthermore, the ratio of the first modified camellia seed oil, glucose, and glutaraldehyde in B2 is 100g: 15-20g: 3-5ml.

[0015] Furthermore, the ratio of octenyl succinate starch ester, Tween 80, deionized water, and second-modified camellia seed oil in B3 is 10-15g: 2-3g: 60-70ml: 100g.

[0016] A method for preparing a diglyceride-based edible oil that helps lower blood sugar and lipids specifically includes the following steps: S1. Place 40-60 parts of high-purity diglyceride oil and 5-8 parts of perilla seed oil in a 60℃ constant temperature stirring pot and preheat for 30 minutes to remove trace amounts of moisture from the oils; mix 2-5 parts of phytosterol esters, 1-3 parts of resistant dextrin, and 0.05-0.15 parts of potassium citrate evenly and grind them into a fine powder with a particle size ≤50μm for later use. S2. Take 2-3 parts of modified flaxseed oil micro powder and 2-3 parts of modified camellia seed oil microcapsule powder, add them to the preheated mixture of diglyceride oil and perilla seed oil, and stir for 30 minutes at a speed of 500 r / min to completely disperse the modified powder. S3. Add the fine powder from S1 that has been mixed evenly to the above mixed oil, heat to 65°C, and continue stirring for 20 minutes; add 0.1-0.5 parts of rosemary extract, stir for 10 minutes, and then place the mixture in a high-pressure homogenizer and homogenize twice under a pressure of 60-70 MPa to ensure that each component is evenly dispersed. S4. Place the homogenized mixed oil in a vacuum degasser and degas it for 15 minutes under a vacuum of -0.09 MPa and a temperature of 50°C. After degassing, allow it to cool naturally to room temperature to obtain the composite edible oil base. S5. The composite edible oil base material is pre-filtered through a 1μm plate and frame filter and then filtered through a 0.45μm polyethersulfone terminal filter element. The filtration conditions are 50℃ and 0.3MPa pressure. The filter element is replaced every 8 hours. The filtered oil is then filled into light-proof bottles under sterile conditions, sealed and stored to obtain the finished product.

[0017] Furthermore, in S3, the high-pressure homogenization employs a gradient pressure operation. The first homogenization pressure is 60 MPa, and the second homogenization pressure is 70 MPa, with each homogenization lasting 3-5 minutes and an interval of 5 minutes between homogenizations. The system temperature is controlled to not exceed 70°C during the homogenization process. Gradient pressure homogenization first initially disperses and then further refines the components, avoiding structural damage caused by a single high pressure. Strict temperature control and time interval design ensure that the activity of the components is not affected during homogenization, while achieving optimal dispersion and guaranteeing product efficacy and stability.

[0018] This invention provides a compound edible oil of diglycerides that helps lower blood sugar and blood lipids, and its preparation method, which has the following beneficial effects: 1. This product uses a scientifically formulated composite raw material system, with high-purity diglyceride oil as the core, combined with modified flaxseed oil micropowder, modified camellia seed oil microcapsule powder, perilla seed oil, and functional ingredients such as phytosterol esters. Each component works synergistically. Diglycerides can reduce fat accumulation and regulate lipid metabolism, while phytosterol esters can inhibit cholesterol absorption. The modified flaxseed oil and camellia seed oil enhance the effects of unsaturated fatty acids and other active ingredients, jointly achieving dual auxiliary regulation of blood sugar and blood lipids. This solves the problem of limited efficacy of single ingredients and meets the dietary needs of people with metabolic diseases.

[0019] 2. Through multiple modification processes, the performance of raw materials is optimized. Flaxseed oil is modified into micro-powder through enzymatic hydrolysis, encapsulation, and fermentation. Camellia seed oil undergoes molecular distillation, UV modification, and microencapsulation, significantly improving the stability of functional components and preventing them from becoming ineffective due to oxidation during processing and storage. Simultaneously, the compatibility of the modified powdered raw materials with the oil system is significantly improved. Combined with high-pressure gradient homogenization and other preparation processes, the problems of uneven component dispersion and easy stratification in traditional products are effectively solved, ensuring consistency in product sensory quality and user experience.

[0020] 3. The modification of raw materials and precise control of the preparation process significantly improve the absorption rate of functional ingredients. The fermentation-encapsulation structure of flaxseed oil micropowder and the ultrafine emulsion microcapsule form of camellia seed oil can protect active ingredients from gastric acid damage and promote their efficient release and absorption in the intestines. Components such as resistant dextrin can also regulate the intestinal environment, further assisting in the absorption and conversion of nutrients. This solves the technical bottleneck of low absorption rate of active ingredients in traditional functional edible oils, making it easier to exert auxiliary regulatory effects.

[0021] 4. The product uses a combination of natural raw materials, with rosemary extract as a natural antioxidant to replace artificial synthetic additives. Potassium citrate is used to regulate the stability of the system. There are no additional harmful additives throughout the entire process, making it safer for consumption. Furthermore, the raw material selection process is rigorous, and the process parameters (such as temperature, pressure, and time) at each stage are clearly standardized. The entire process, from raw material modification to finished product filling, is controllable, effectively avoiding the problem of large quality fluctuations in traditional products and ensuring that the efficacy, stability, and safety of each batch of products meet consistent standards. Detailed Implementation

[0022] The technical solutions in the embodiments of the present invention will be clearly and completely described below. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0023] Example 1: Preparation of a diglyceride complex edible oil that helps lower blood sugar and blood lipids. The specific preparation steps are as follows: S1. Place 40 parts of high-purity diglyceride oil and 5 parts of perilla seed oil in a 60℃ constant temperature stirring pot and preheat for 30 minutes to remove trace amounts of moisture from the oils; mix 2 parts of phytosterol ester, 1 part of resistant dextrin and 0.05 parts of potassium citrate evenly and grind them into fine powder with a particle size ≤50μm for later use. S2. Take 2 parts of modified flaxseed oil micro powder and 2 parts of modified camellia seed oil microcapsule powder, add them to the preheated mixture of diglyceride oil and perilla seed oil, and stir for 30 minutes at a speed of 500 r / min to completely disperse the modified powder. S3. Add the fine powder from S1 that has been mixed evenly to the above mixed oil, heat to 65°C, and continue stirring for 20 minutes; add 0.1 parts of rosemary extract, stir for 10 minutes, and then place the mixture in a high-pressure homogenizer. The first homogenization pressure is 60 MPa, the second homogenization pressure is 70 MPa, the homogenization time is 3 minutes each time, and the interval between the two homogenizations is 5 minutes. During the homogenization process, the system temperature is controlled not to exceed 70°C to ensure that the components are evenly dispersed. S4. Place the homogenized mixed oil in a vacuum degasser and degas it for 15 minutes under a vacuum of -0.09 MPa and a temperature of 50°C. After degassing, allow it to cool naturally to room temperature to obtain the composite edible oil base. S5. The composite edible oil base material is pre-filtered through a 1μm plate and frame filter and then filtered through a 0.45μm polyethersulfone terminal filter element. The filtration conditions are 50℃ and 0.3MPa pressure. The filter element is replaced every 8 hours. The filtered oil is then filled into light-proof bottles under sterile conditions, sealed and stored to obtain the finished product.

[0024] Example 2: Preparation of a diglyceride complex edible oil that helps lower blood sugar and blood lipids. The specific preparation steps are as follows: S1. Place 60 parts of high-purity diglyceride oil and 8 parts of perilla seed oil in a constant temperature stirring pot at 60℃ and preheat for 30 minutes to remove trace amounts of moisture from the oils; mix 5 parts of phytosterol ester, 3 parts of resistant dextrin and 0.15 parts of potassium citrate evenly and grind them into fine powder with a particle size ≤50μm for later use. S2. Take 3 parts of modified flaxseed oil micro powder and 3 parts of modified camellia seed oil microcapsule powder, add them to the preheated mixture of diglyceride oil and perilla seed oil, and stir for 30 minutes at a speed of 500 r / min to completely disperse the modified powder. S3. Add the fine powder from S1 that has been mixed evenly to the above mixed oil, heat to 65°C, and continue stirring for 20 minutes; add 0.5 parts of rosemary extract, stir for 10 minutes, and then place the mixture in a high-pressure homogenizer. The first homogenization pressure is 60 MPa, the second homogenization pressure is 70 MPa, the homogenization time is 5 minutes each time, and the interval between the two homogenizations is 5 minutes. During the homogenization process, the system temperature is controlled not to exceed 70°C to ensure that the components are evenly dispersed. S4. Place the homogenized mixed oil in a vacuum degasser and degas it for 15 minutes under a vacuum of -0.09 MPa and a temperature of 50°C. After degassing, allow it to cool naturally to room temperature to obtain the composite edible oil base. S5. The composite edible oil base material is pre-filtered through a 1μm plate and frame filter and then filtered through a 0.45μm polyethersulfone terminal filter element. The filtration conditions are 50℃ and 0.3MPa pressure. The filter element is replaced every 8 hours. The filtered oil is then filled into light-proof bottles under sterile conditions, sealed and stored to obtain the finished product.

[0025] Example 3: Preparation of a diglyceride complex edible oil that helps lower blood sugar and blood lipids. The specific preparation steps are as follows: S1. Place 50 parts of high-purity diglyceride oil and 6 parts of perilla seed oil in a 60℃ constant temperature stirring pot and preheat for 30 minutes to remove trace amounts of moisture from the oils; mix 3 parts of phytosterol ester, 2 parts of resistant dextrin and 0.1 parts of potassium citrate evenly and grind them into fine powder with a particle size ≤50μm for later use. S2. Take 2.5 parts of modified flaxseed oil micro powder and 2.5 parts of modified camellia seed oil microcapsule powder, add them to the preheated mixture of diglyceride oil and perilla seed oil, and stir for 30 minutes at a speed of 500 r / min to completely disperse the modified powder. S3. Add the fine powder from S1 that has been mixed evenly to the above mixed oil, heat to 65°C, and continue stirring for 20 minutes; add 0.3 parts of rosemary extract, stir for 10 minutes, and then place the mixture in a high-pressure homogenizer. The first homogenization pressure is 60 MPa, the second homogenization pressure is 70 MPa, the homogenization time is 4 minutes each time, and the interval between the two homogenizations is 5 minutes. During the homogenization process, the system temperature is controlled not to exceed 70°C to ensure that the components are evenly dispersed. S4. Place the homogenized mixed oil in a vacuum degasser and degas it for 15 minutes under a vacuum of -0.09 MPa and a temperature of 50°C. After degassing, allow it to cool naturally to room temperature to obtain the composite edible oil base. S5. The composite edible oil base material is pre-filtered through a 1μm plate and frame filter and then filtered through a 0.45μm polyethersulfone terminal filter element. The filtration conditions are 50℃ and 0.3MPa pressure. The filter element is replaced every 8 hours. The filtered oil is then filled into light-proof bottles under sterile conditions, sealed and stored to obtain the finished product.

[0026] Example 4: Preparation of modified flaxseed oil micro powder. The specific preparation steps are as follows: A1. Flaxseed oil was washed with hot water at 80℃ for 30 min, centrifuged, and vacuum dried. 100 g of the treated flaxseed oil, 30 ml of anhydrous ethanol, and 50 ml of phosphate buffer (pH 6) were mixed. 5 g of immobilized Rhizopus oryzae lipase was added, and the mixture was stirred at 300 r / min for 4 h at 45℃ and 200 W ultrasonic power. 10 g of citric acid was added to the system, the temperature was raised to 55℃, and 5 ml of 30% hydrogen peroxide solution was slowly added dropwise. The mixture was stirred at 200 r / min for 2 h. After the reaction was completed, the mixture was centrifuged at 4000 r / min for 10 min, washed with water, and then distilled under reduced pressure at -0.09 MPa and 60℃ to remove the solvent, thus obtaining the first modified flaxseed oil. A2. Mix 100g of first-modified flaxseed oil with 15g of β-cyclodextrin and 80ml of deionized water, heat to 70℃, and stir at 250r / min for 1.5h. After the reaction, cool to room temperature, centrifuge at 4000r / min for 10min, freeze dry under vacuum of -0.1MPa and -40℃, and pulverize to obtain second-modified flaxseed oil powder. A3. Mix 100g of second-modified flaxseed oil powder, 5g of glucose, and 60ml of probiotic liquid, and let it ferment at 35℃, normal pressure, and nitrogen protection for 12 hours. After fermentation, add 10g of maltodextrin to the system and dry it in a spray dryer with the inlet air temperature set at 120℃ and the outlet air temperature at 60℃ to obtain modified flaxseed oil micro powder.

[0027] Example 5: Preparation of modified flaxseed oil micro powder. The specific preparation steps are as follows: A1. Flaxseed oil was washed with hot water at 80℃ for 30 min, centrifuged, and vacuum dried. 100 g of the treated flaxseed oil, 40 ml of anhydrous ethanol, and 60 ml of phosphate buffer (pH 6) were mixed. 8 g of immobilized Rhizopus oryzae lipase was added, and the mixture was stirred at 400 r / min for 4 h at 50℃ and 300 W ultrasonic power. 15 g of citric acid was added to the system, the temperature was raised to 55℃, and 8 ml of 30% hydrogen peroxide solution was slowly added dropwise. The mixture was stirred at 250 r / min for 2 h. After the reaction was completed, the mixture was centrifuged at 4000 r / min for 10 min, washed with water, and then distilled under reduced pressure at -0.09 MPa and 60℃ to remove the solvent, thus obtaining the first modified flaxseed oil. A2. Mix 100g of first-modified flaxseed oil with 20g of β-cyclodextrin and 100ml of deionized water, heat to 70℃, and stir at 300r / min for 1.5h. After the reaction, cool to room temperature, centrifuge at 4000r / min for 10min, freeze dry under vacuum of -0.1MPa and -40℃, and pulverize to obtain second-modified flaxseed oil powder. A3. Mix 100g of second-modified flaxseed oil powder, 8g of glucose, and 70ml of probiotic liquid, and let it ferment at 35℃, normal pressure, and nitrogen protection for 12 hours. After fermentation, add 15g of maltodextrin to the system and dry it in a spray dryer with the inlet air temperature set at 120℃ and the outlet air temperature at 60℃ to obtain modified flaxseed oil micro powder.

[0028] Example 6: Preparation of modified camellia seed oil microcapsule powder. The specific preparation steps are as follows: B1. Camellia seed oil was subjected to molecular distillation at a vacuum of -0.095 MPa and a temperature of 180℃ to remove tea saponins. 100g of the treated camellia seed oil was mixed with 100ml of 70% ethanol solution and homogenized twice at a pressure of 40MPa for 5min each time in a high-pressure homogenizer. 4g of immobilized Rhizopus oryzae lipase and 8ml of acetic anhydride were added to the homogenate and stirred at 250r / min for 3h at 40℃. Sodium carbonate was added to neutralize to pH 7.0. Ethanol was removed by vacuum distillation and then centrifuged at 3000r / min for 15min to obtain the first modified camellia seed oil. B2. Mix 100g of first-modified camellia seed oil with 15g of glucose, add 0.1mol / L hydrochloric acid to adjust the pH to 3.0, and react at 200r / min for 2h under ultraviolet light irradiation at a wavelength of 254nm, power of 30W, and a distance of 20cm. Add 3ml of 25% glutaraldehyde to the system, adjust the pH to 8.0 with 0.5mol / L sodium hydroxide, heat to 50℃, and react at 300r / min for 1.5h. After the reaction, wash three times with deionized water and dry under reduced pressure at -0.08MPa and 50℃ to obtain second-modified camellia seed oil. B3. Dissolve 10g of octenyl succinic starch ester and 2g of Tween 80 in 60ml of deionized water and stir until homogeneous to obtain an aqueous phase. Use 100g of second-modified camellia seed oil as the oil phase and slowly add it to the aqueous phase. Shear at 10000r / min for 10min to obtain a primary emulsion. Place the primary emulsion in a high-pressure microjet mill and homogenize it three times at 80MPa to form an ultrafine emulsion. Spray dry the ultrafine emulsion with an inlet air temperature of 170℃ and an outlet air temperature of 75℃. Then place the spray-dried powder in an air jet mill and depolymerize it at 0.6MPa for 5min, controlling the secondary particle size D50 to be 0.5-1μm to obtain modified camellia seed oil microcapsule powder.

[0029] Example 7: Preparation of modified camellia seed oil microcapsule powder. The specific preparation steps are as follows: B1. Camellia seed oil was subjected to molecular distillation at a vacuum of -0.095 MPa and a temperature of 180℃ to remove tea saponins. 100g of the treated camellia seed oil was mixed with 120ml of 70% ethanol solution and homogenized twice at a pressure of 50MPa for 5min each time in a high-pressure homogenizer. 6g of immobilized Rhizopus oryzae lipase and 10ml of acetic anhydride were added to the homogenate and stirred at 300r / min for 3h at 40℃. Sodium carbonate was added to neutralize to pH 7.0. Ethanol was removed by vacuum distillation and then centrifuged at 3000r / min for 15min to obtain the first modified camellia seed oil. B2. Mix 100g of first-modified camellia seed oil with 20g of glucose, add 0.1mol / L hydrochloric acid to adjust the pH to 3.0, and react with stirring at 250r / min for 2h under ultraviolet light irradiation at a wavelength of 254nm, power of 30W, and a distance of 20cm. Add 5ml of 25% glutaraldehyde to the system, adjust the pH to 8.0 with 0.5mol / L sodium hydroxide, heat to 50℃, and react with stirring at 350r / min for 1.5h. After the reaction, wash three times with deionized water and dry under reduced pressure at -0.08MPa and 50℃ to obtain second-modified camellia seed oil. B3. Dissolve 15g of octenyl succinic starch ester and 3g of Tween 80 in 70ml of deionized water and stir until homogeneous to obtain an aqueous phase. Use 100g of second-modified camellia seed oil as the oil phase and slowly add it to the aqueous phase. Shear at 10000r / min for 10min to obtain a primary emulsion. Place the primary emulsion in a high-pressure microjet mill and homogenize it three times at 100MPa to form an ultrafine emulsion. Spray dry the ultrafine emulsion with an inlet air temperature of 170℃ and an outlet air temperature of 75℃. Then place the spray-dried powder in an air jet mill and depolymerize it at 0.6MPa for 5min, controlling the secondary particle size D50 to be 0.5-1μm to obtain modified camellia seed oil microcapsule powder.

[0030] Comparative Example 1: A compound edible oil containing diglycerides that helps lower blood sugar and blood lipids was prepared. The specific preparation steps are as follows: The remaining steps remain unchanged, except that the modified flaxseed oil powder prepared in Example 4 and used in Example 3 are replaced with unmodified flaxseed oil powder that has only been simply dried and pulverized, in order to prepare a diglyceride complex edible oil that helps lower blood sugar and blood lipids.

[0031] Comparative Example 2: A compound edible oil containing diglycerides that helps lower blood sugar and blood lipids was prepared. The specific preparation steps are as follows: The remaining steps remain unchanged, except that the modified camellia seed oil microcapsule powder prepared in Example 7 and used in Example 3 is replaced with unmodified camellia seed oil that has only been simply dried and pulverized, in order to prepare a triglyceride complex edible oil that helps lower blood sugar and blood lipids.

[0032] Comparative Example 3: A diglyceride complex edible oil was prepared to help lower blood sugar and blood lipids. The specific preparation steps are as follows: The remaining steps remain unchanged, except that the modified flaxseed oil micropowder prepared in Example 4 and used in Example 3 are replaced with unmodified flaxseed oil micropowder that has only been simply dried and pulverized, and the modified camellia seed oil microcapsule powder prepared in Example 7 is replaced with unmodified camellia seed oil that has only been simply dried and pulverized, so as to prepare a triglyceride complex edible oil that helps lower blood sugar and blood lipids.

[0033] Performance testing Animal model testing Modeling: Sixty SPF-grade SD rats were selected and fed a high-fat diet for 4 weeks. Then, they were injected intraperitoneally with 40 mg / kg streptozotocin to construct a type II diabetes mellitus combined with hyperlipidemia model. Forty-eight rats with fasting blood glucose (FBG) ≥11.1 mmol / L and total cholesterol (TC) ≥5.2 mmol / L were selected as model rats.

[0034] Grouping: Animals were randomly divided into a blank control group, Example 1 group, Example 2 group, Example 3 group, Comparative Example 1 group, Comparative Example 2 group, and Comparative Example 3 group, with 8 animals in each group. The blank control group was given a basal diet, while the other groups received 1.5 g / kg of feed daily.-1 ·d -1 The corresponding samples were administered via gavage for 28 consecutive days.

[0035] Testing: After the experiment, the participants fasted for 12 hours, and blood was drawn from the orbital fossa to measure serum total cholesterol (TC), triglycerides (TG), and fasting blood glucose (FBG) (data were statistically analyzed, and the differences between groups were P<0.01); liver tissue was taken to measure liver glycogen content; and the retention rate of core components was tested after 6 months of storage.

[0036] Test index Blank control group Example 1 Example 2 Example 3 Comparative Example 1 Comparative Example 2 Comparative Example 3 TC reduction rate (%) -3.2 13.8 16.1 19.8 9.2 11.2 6.6 TG reduction rate (%) -2.8 15.4 17.9 20.6 10.0 12.4 7.0 FBG reduction rate (%) -4.5 9.5 11.5 14.2 5.6 6.9 3.8 Liver glycogen content (mg / g liver tissue) 14.2±3.1 17.2±3.3 18.9±3.5 21.1±3.8 14.9±3.0 15.8±3.2 14.5±2.9 Functional ingredient retention rate (6 months of storage, %) / 89.2 91.5 94.3 72.6 76.8 68.4 Statistical difference (with blank control group) / P<0.01 P<0.01 P<0.01 P<0.01 P<0.01 P>0.05 In a 28-day performance test on rats with type 2 diabetes and hyperlipidemia, groups 1-3 (using a compound edible oil made from modified flaxseed oil micropowder and modified camellia seed oil microcapsule powder) showed significant effects in assisting in lowering blood lipids, blood sugar, and increasing liver glycogen content. Total cholesterol (TC) decreased by 6.6%-19.8%, triglycerides (TG) by 7.0%-20.6%, fasting blood glucose (FBG) by 3.8%-14.2%, and liver glycogen content reached a maximum of 21.1±3.8 mg / g liver tissue, with improved storage capacity. After 6 months, the retention rate of functional components reached 89.2%-94.3%, and the system stability was good. The difference was extremely significant compared with the blank control group (P<0.01). However, the efficacy indicators and component retention rates of comparative groups 1-3 (replaced with unmodified flaxseed oil micropowder, unmodified camellia seed oil microcapsule powder, or both) were significantly lower than those of the example group. Among them, comparative group 3 had no significant difference from the blank control group (P>0.05). This fully demonstrates that the modification treatment of compound edible oil raw materials helps postprandial lipid metabolism and helps maintain normal blood glucose fluctuation range.

[0037] The above description is merely an example and illustration of the present invention. Those skilled in the art can make various modifications or additions to the specific embodiments described, or use similar methods to replace them, as long as they do not deviate from the invention or exceed the scope defined in the claims, all of which should fall within the protection scope of the present invention.

Claims

1. A compound edible oil containing diglycerides that helps lower blood sugar and blood lipids, characterized in that: It contains the following ingredients by weight: 40-60 parts high-purity diglyceride oil, 2-3 parts modified flaxseed oil micro powder, 2-3 parts modified camellia seed oil microcapsule powder, 5-8 parts perilla seed oil, 2-5 parts phytosterol esters, 1-3 parts resistant dextrin, 0.1-0.5 parts rosemary extract, and 0.05-0.15 parts potassium citrate.

2. The diglyceride compound edible oil for assisting in lowering blood sugar and blood lipids according to claim 1, characterized in that: The purity of the high-purity diglyceride oil is ≥90%.

3. The diglyceride compound edible oil for assisting in lowering blood sugar and blood lipids according to claim 1, characterized in that: The modified flaxseed oil powder is prepared using the following specific steps: A1. Flaxseed oil was washed with hot water at 80℃ for 30 min, centrifuged, and vacuum dried. The treated flaxseed oil, anhydrous ethanol, and phosphate buffer at pH 6 were mixed, and immobilized Rhizopus oryzae lipase was added. The mixture was stirred at 300-400 r / min for 4 h at 45-50℃ and ultrasonic power of 200-300 W. Citric acid was added to the system, the temperature was raised to 55℃, and 30% hydrogen peroxide solution was slowly added dropwise. The mixture was stirred at 200-250 r / min for 2 h. After the reaction was completed, the mixture was centrifuged at 4000 r / min for 10 min, washed with water, and then distilled under reduced pressure at -0.09 MPa and 60℃ to remove the solvent, thus obtaining the first modified flaxseed oil. A2. The first modified flaxseed oil was mixed with β-cyclodextrin and deionized water, heated to 70℃, and stirred at 250-300 r / min for 1.5 h. After the reaction, it was cooled to room temperature, centrifuged at 4000 r / min for 10 min, and then freeze-dried under vacuum of -0.1 MPa and -40℃. After pulverizing, the second modified flaxseed oil powder was obtained. A3. Mix the second modified flaxseed oil powder, glucose, and probiotic liquid, and let it ferment at 35°C, normal pressure, and nitrogen protection for 12 hours. After fermentation, add maltodextrin to the system and dry it in a spray dryer with the inlet air temperature set at 120°C and the outlet air temperature at 60°C to obtain modified flaxseed oil micro powder.

4. The diglyceride compound edible oil for assisting in lowering blood sugar and blood lipids according to claim 3, characterized in that: The ratio of flaxseed oil, anhydrous ethanol, phosphate buffer, immobilized Rhizopus oryzae lipase, citric acid, and hydrogen peroxide solution in A1 is 100g:30-40ml:50-60ml:5-8g:10-15g:5-8ml; the immobilized Rhizopus oryzae lipase activity is 240-250U / g. The ratio of the first modified flaxseed oil, β-cyclodextrin, and deionized water in A2 is 100g: 15-20g: 80-100ml; The ratio of the modified flaxseed oil powder, glucose, probiotic solution, and maltodextrin in A3 is 100g: 5-8g: 60-70ml: 10-15g; the probiotic solution is a mixed solution of Lactobacillus acidophilus and Lactobacillus plantarum, with a viable count ≥1.2×10⁻⁶. 9 CFU / ml.

5. The diglyceride compound edible oil for assisting in lowering blood sugar and blood lipids according to claim 1, characterized in that: The modified camellia seed oil microcapsule powder is prepared using the following specific steps: B1. Camellia seed oil was subjected to molecular distillation at a vacuum of -0.095 MPa and a temperature of 180℃ to remove tea saponins. The treated camellia seed oil was mixed with a 70% (v / v) ethanol solution and homogenized twice in a high-pressure homogenizer at a pressure of 40-50 MPa for 5 min each time. Immobilized Rhizopus oryzae lipase and acetic anhydride were added to the homogenized solution and stirred at 250-300 r / min for 3 h at 40℃. Sodium carbonate was added to neutralize to pH 7.

0. Ethanol was removed by vacuum distillation and then centrifuged at 3000 r / min for 15 min to obtain the first modified camellia seed oil. B2. The first-modified camellia seed oil was mixed with glucose, and 0.1 mol / L hydrochloric acid was added to adjust the pH to 3.

0. Under ultraviolet light with a wavelength of 254 nm, a power of 30 W, and a distance of 20 cm, the mixture was stirred at 200-250 r / min for 2 h. 25% glutaraldehyde was added to the system, and the pH was adjusted to 8.0 with 0.5 mol / L sodium hydroxide. The temperature was raised to 50 °C, and the mixture was stirred at 300-350 r / min for 1.5 h. After the reaction, the mixture was washed three times with deionized water and dried under reduced pressure at -0.08 MPa and 50 °C to obtain the second-modified camellia seed oil. B3. Dissolve octenyl succinic acid starch ester and Tween 80 in deionized water and stir until homogeneous to obtain an aqueous phase. Use the second modified camellia seed oil as the oil phase and slowly add it to the aqueous phase. Shear at 10000 r / min for 10 min to obtain a primary emulsion. Place the primary emulsion in a high-pressure microjet mill and homogenize it three times under a pressure of 80-100 MPa to form an ultrafine emulsion. Spray dry the ultrafine emulsion with an inlet air temperature of 170℃ and an outlet air temperature of 75℃. Then place the spray-dried powder in an air jet mill and depolymerize it under a pressure of 0.6 MPa for 5 min. Control the secondary particle size D50 to be 0.5-1 μm to obtain modified camellia seed oil microcapsule powder.

6. The diglyceride compound edible oil for assisting in lowering blood sugar and blood lipids according to claim 5, characterized in that: The ratio of camellia seed oil, ethanol solution, immobilized Rhizopus oryzae lipase, and acetic anhydride in B1 is 100g: 100-120ml: 4-6g: 8-10ml; the immobilized Rhizopus oryzae lipase has an activity of 240-250U / g. The ratio of the first modified camellia seed oil, glucose, and glutaraldehyde in B2 is 100g: 15-20g: 3-5ml; The ratio of octenyl succinic starch ester, Tween 80, deionized water, and second-modified camellia seed oil in B3 is 10-15g: 2-3g: 60-70ml: 100g.

7. A method for preparing a diglyceride compound edible oil that helps lower blood sugar and blood lipids, characterized in that: Specifically, it includes the following steps: S1. Place 40-60 parts of high-purity diglyceride oil and 5-8 parts of perilla seed oil in a 60℃ constant temperature stirring pot and preheat for 30 minutes to remove trace amounts of moisture from the oils; mix 2-5 parts of phytosterol esters, 1-3 parts of resistant dextrin, and 0.05-0.15 parts of potassium citrate evenly and grind them into a fine powder with a particle size ≤50μm for later use. S2. Take 2-3 parts of modified flaxseed oil micro powder and 2-3 parts of modified camellia seed oil microcapsule powder, add them to the preheated mixture of diglyceride oil and perilla seed oil, and stir for 30 minutes at a speed of 500 r / min to completely disperse the modified powder. S3. Add the fine powder from S1 that has been mixed evenly to the above mixed oil, heat to 65°C, and continue stirring for 20 minutes; add 0.1-0.5 parts of rosemary extract, stir for 10 minutes, and then place the mixture in a high-pressure homogenizer and homogenize twice under a pressure of 60-70 MPa to ensure that each component is evenly dispersed. S4. Place the homogenized mixed oil in a vacuum degasser and degas it for 15 minutes under a vacuum of -0.09 MPa and a temperature of 50°C. After degassing, allow it to cool naturally to room temperature to obtain the composite edible oil base. S5. The composite edible oil base material is pre-filtered through a 1μm plate and frame filter and then filtered through a 0.45μm polyethersulfone terminal filter element. The filtration conditions are 50℃ and 0.3MPa pressure. The filter element is replaced every 8 hours. The filtered oil is then filled into light-proof bottles under sterile conditions, sealed and stored to obtain the finished product.

8. The method for preparing a diglyceride compound edible oil for assisting in lowering blood sugar and lipids according to claim 7, characterized in that: The high-pressure homogenization in S3 adopts a gradient pressure operation. The first homogenization pressure is 60MPa, the second homogenization pressure is 70MPa, the homogenization time is 3-5min, the interval between two homogenizations is 5min, and the system temperature is controlled not to exceed 70℃ during the homogenization process.