Preparation method and application of metagen product containing ergothioneine and achyranthes bidentata polysaccharide and capable of improving immunity

Abstract

The invention discloses a preparation method and application of a metagen product containing ergothioneine and achyranthes bidentata polysaccharides and capable of improving immunity, and relates to the technical field of microbial fermentation engineering and functional food application. The invention discloses a metagen product containing ergothioneine and achyranthes bidentata polysaccharide and capable of improving immunity. The metagen product is prepared from the following components in parts by mass: 80-100 parts of lactobacillus plantarum thallus powder containing ergothioneine, 10-16 parts of achyranthes bidentata polysaccharide, 0.5-1.5 parts of an immunoactivator, 20-40 parts of prebiotics, 3-5 parts of a sulfur element precursor and 1-10 parts of an emulsion stabilizer. And the specific immunoactivator and inactivated thalli form a dual anti-oxidation barrier, so that the oxidative degradation of ergothioneine is reduced, and the stability of the product is improved. The superfine ground thalli are matched with prebiotics and an emulsion stabilizer, so that release of active ingredients and absorption of intestinal tracts are promoted, and the bioavailability is improved. Multiple components are scientifically matched to achieve synergistic interaction, intestinal regulation and system immunity enhancement are both considered, and the efficacy is more comprehensive.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of microorganisms and functional food application, and in particular to a preparation method and application of a postbiotic product containing ergothioneine and radix cyathulae polysaccharide for improving immunity. BACKGROUND

[0002] Immunity is the core guarantee for the body to resist external pathogens and maintain the stability of the internal environment. With the acceleration of life pace, the increase of pressure and the aggravation of aging, the problem of declining immunity in the population is increasingly prominent. Health problems such as fatigue, susceptibility to infection, etc. frequently occur, and the demand for functional foods and health products continues to rise. Postbiotics, as a general term for inactivated microorganisms and their metabolites, have become a research hotspot in the field of improving immunity due to their high safety, strong stability, and wide applicability to people.

[0003] Ergothioneine is a natural antioxidant with the functions of scavenging free radicals, protecting cells from oxidative damage, and regulating immune cell activity. It has good application prospects when combined with postbiotics to develop related products. However, there are many technical bottlenecks in the existing technology of ergothioneine postbiotic products: first, ergothioneine molecules are sensitive to oxidation and are easily degraded during production, storage, and digestion in the human body, resulting in a short shelf life of the product and a serious loss of core functional ingredients; second, the cell wall structure of Lactobacillus plantarum is dense, making it difficult for ergothioneine to be released, and the complex gastrointestinal environment further reduces the bioavailability of active ingredients and their absorption and utilization; third, the formula design lacks scientific compatibility and relies mainly on single ingredients to exert their effects, failing to achieve the synergistic effect of intestinal regulation and systemic immune enhancement, resulting in single efficacy and insufficient targeting.

[0004] At the same time, the compatibility of traditional antioxidants with ergothioneine is poor, and some chemical antioxidants are controversial in terms of safety. The dosage and ratio of auxiliary ingredients such as prebiotics and sulfur element precursors are not optimized, further limiting the overall performance of the product. Therefore, developing an ergothioneine postbiotic product with strong stability, high bioavailability, and multi-target synergistic effect has become a key direction to solve the defects of existing technology and meet market demand. SUMMARY

[0005] In view of the problems of poor stability (ergothioneine is easily oxidized and degraded), low bioavailability (the dense cell wall of the bacteria makes it difficult for active ingredients to be released), single efficacy (lack of multi-ingredient synergistic effect), and safety concerns of some antioxidants in existing ergothioneine postbiotic products, a kind of ergothioneine postbiotic product with strong stability, high bioavailability, and multi-target synergistic effect for improving immunity and relieving physical fatigue is provided, as well as its preparation method and application.

[0006] To achieve the above-mentioned purposes, the technical solution adopted by the present application is as follows: A postbiotic product containing ergothioneine and astragalus polysaccharide for improving immunity is prepared from the following components: Lactobacillus plantarum powder containing ergothioneine 80-100 parts, astragalus polysaccharide 10-16 parts, immune active agent 0.5-1.5 parts, prebiotic 20-40 parts, sulfur element precursor 3-5 parts, and emulsion stabilizer 1-10 parts. The immune active agent is a compound shown in formula 1. The structure of formula 1 is: ; R1 in formula 1 is selected from any one of hydroxyl, alkyl with carbon atoms in the range of 1-5, alkoxy with carbon atoms in the range of 1-5, or aryl with carbon atoms in the range of 6-15.

[0007] Further, the Lactobacillus plantarum powder containing ergothioneine is a mixture of Lactobacillus plantarum TWK-10 and ergothioneine, and the mass ratio of the two is 20:1. The Lactobacillus plantarum TWK-10 powder is an inactivated bacterial body at 121℃ for 15 minutes.

[0008] Further, the immune active agent is any one of the compounds shown in the following structural formula: ; ; .

[0009] Further, the prebiotic is at least one of fructooligosaccharide, inulin, and resistant dextrin.

[0010] Further, the sulfur element precursor is any one of magnesium sulfate, glutathione, or thiamine.

[0011] Further, the emulsion stabilizer is at least one of gum arabic, pectin, and carrageenan.

[0012] Further, a preparation method of a postbiotic product containing ergothioneine and astragalus polysaccharide for improving immunity comprises the following steps: S1. Mixing the Lactobacillus plantarum TWK-10 and the ergothioneine, and grinding to a powder with a particle size not less than 200 mesh, and sieving to obtain the Lactobacillus plantarum powder containing ergothioneine; S2. Placing the Lactobacillus plantarum powder containing ergothioneine, astragalus polysaccharide, immune active agent, prebiotic, sulfur element precursor, and optional emulsion stabilizer in a mixing device to mix uniformly, and then drying to obtain the postbiotic product containing ergothioneine and astragalus polysaccharide for improving immunity.

[0013] Further, the S1 is performed under a nitrogen atmosphere.

[0014] Further, the drying treatment in S2 is vacuum freeze drying or spray drying.

[0015] Further, the application of the ergothioneine and polysaccharide-containing probiotic product of Achyranthes bidentata in improving immunity and relieving physical fatigue in the preparation of food, health care products or dietary supplements for improving immunity and relieving physical fatigue.

[0016] The present application forms a synergistic effect through scientific compounding of specific mass fractions of components, and precisely overcomes the bottleneck of the prior art: the core component contains Lactobacillus plantarum cell powder containing ergothioneine, which is matched with an immune active agent with a specific structure, and the two form a double antioxidant barrier and improve immunity; the prebiotics, emulsifying stabilizers and cell powder synergize, the prebiotics regulate the intestinal microecological environment, the emulsifying stabilizers optimize the dispersion of the system, the ultrafine grinding destroys the dense cell wall of the bacteria, and the three promote the release of active ingredients such as ergothioneine together, and at the same time improve the tolerance and absorption efficiency of the active ingredients in the gastrointestinal tract, solving the problem of low bioavailability; the sulfur element precursor further assists ergothioneine in playing an immune regulatory role, and forms a multi-target synergistic effect with the core component, the immune active agent and the prebiotics, which regulates the intestinal immunity by means of the probiotic properties of Lactobacillus plantarum, and activates the immune cell activity through ergothioneine and the immune active agent, realizing the dual functions of intestinal regulation and systemic immune enhancement, and making up for the single function defect of traditional products.

[0017] Compared with the prior art, the present application has the following beneficial effects: the specific immune active agent and the inactivated bacteria form a double antioxidant barrier, reduce the oxidative degradation of ergothioneine, and improve the stability of the product. The ultrafine grinding of the bacteria is matched with the prebiotics and emulsifying stabilizers to promote the release and intestinal absorption of active ingredients and improve the bioavailability. Scientific compounding of multiple components realizes synergistic effect, and takes into account intestinal regulation and systemic immune enhancement, and the function is more comprehensive. BRIEF DESCRIPTION OF DRAWINGS Figure 1 It is a preparation method of the immune active agent of the present application.

Claims

1. A postbiotic product containing ergothioneine and polysaccharides from Cyathula officinalis for improving immunity, characterized in that, is prepared from the following components: Lactobacillus plantarum cell powder containing ergothioneine 80-100 parts, Achyranthes bidentata polysaccharide 10-16 parts, an immunologically active agent 0.5-1.5 parts, a prebiotic 20-40 parts, a sulfur element precursor 3-5 parts, and an emulsion stabilizer 1-10 parts; the immunologically active agent is a compound represented by Formula 1; The structure of Formula 1 is: ; R1 in Formula 1 is selected from any one of a hydroxyl group, an alkyl group having 1-5 carbon atoms, an alkoxy group having 1-5 carbon atoms, or an aryl group having 6-15 carbon atoms.

2. The ergogenic product containing ergothioneine and polysaccharides from Achyranthes bidentata according to claim 1, characterized in that, the Lactobacillus plantarum cell powder containing ergothioneine is a mixture of Lactobacillus plantarum TWK-10 and ergothioneine, with a mass ratio of 20:1; the Lactobacillus plantarum TWK-10 cell powder is an inactivated cell at 121°C for 15 min. 3.The ergogenic pruduct containing ergothioneine and pachymaran for improving immunity according to claim 1, characterized in that, the immunologically active agent is any one of the compounds represented by the following structural formulae: ； ； 。 4. The ergogenic product containing ergothioneine and polysaccharides from Achyranthes bidentata according to claim 1, characterized in that, the prebiotic is at least one of fructo-oligosaccharide, inulin, and resistant dextrin.

5. The ergogenic product containing ergothioneine and polysaccharides from Achyranthes bidentata according to claim 1, characterized in that, the sulfur element precursor is any one of magnesium sulfate, glutathione, or thiamine.

6. The ergogenic product containing ergothioneine and polysaccharides from Achyranthes bidentata according to claim 1, characterized in that, the emulsion stabilizer is at least one of gum arabic, pectin, and carrageenan.

7. A method for preparing a postbiotic product containing ergothioneine and polysaccharides from Achyranthes bidentata Blume for improving immunity according to any one of claims 1 to 6, characterized in that, comprising the following steps: S1. mixing the Lactobacillus plantarum TWK-10 and the ergothioneine, grinding to a powder with a particle size no less than 200 mesh, and sieving to obtain the Lactobacillus plantarum cell powder containing ergothioneine; S2. placing the Lactobacillus plantarum cell powder containing ergothioneine, the Achyranthes bidentata polysaccharide, the immunologically active agent, the prebiotic, the sulfur element precursor, and optionally the emulsion stabilizer in a mixing device, mixing uniformly, and then drying to obtain the postbiotic product containing ergothioneine and Achyranthes bidentata polysaccharide for enhancing immunity.

8. The method according to claim 7, wherein the ergothioneine and the polysaccharides from Achyranthes bidentata are used to prepare the postbiotic product for improving immunity. the S1 is performed under a nitrogen atmosphere.

9. The method according to claim 7, wherein the ergothioneine and the polysaccharides from Achyranthes bidentata are used to prepare the postbiotic product for improving immunity. the drying in S2 is vacuum freeze-drying or spray-drying.

10. Use of the postbiotic product containing ergothioneine and Achyranthes bidentata polysaccharide for enhancing immunity according to any one of claims 1-6 in the preparation of a food, health product, or dietary supplement for enhancing immunity and relieving physical fatigue.

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