Anti-oxidation and anti-aging composition containing dendrobium officinale polysaccharide and application of anti-oxidation and anti-aging composition in beauty and health care products

Abstract

The invention relates to the technical field of health care product preparation, in particular to an anti-oxidation and anti-aging composition containing dendrobium officinale polysaccharide and application of the anti-oxidation and anti-aging composition in beauty and health care products. Comprising the following steps: mixing dendrobium officinale with water, extracting, filtering to obtain a filtrate, concentrating to obtain a concentrated solution, and carrying out alcohol precipitation to obtain a precipitate; extracting the precipitate with an extracting agent, concentrating under reduced pressure, and freeze-drying to obtain crude polysaccharide; and dissolving the crude polysaccharide with water, and sequentially loading on an ion exchange column and a gel column for purification to obtain the dendrobium officinale polysaccharide. According to the anti-oxidation and anti-aging composition containing the dendrobium officinale polysaccharide, provided by the invention, an efficient comprehensive anti-aging function is realized through a multi-target and multi-channel synergistic effect. The composition not only can strongly remove various free radicals such as DPPH free radicals, hydroxyl free radicals and superoxide anions and relieve oxidative stress injury, but also can effectively inhibit generation of melanin, has definite whitening and spot-fading effects, and is wide in application prospect.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of health care products, and particularly relates to an antioxidant anti-aging composition containing Dendrobium officinale polysaccharide and application thereof in beauty care products. BACKGROUND

[0002] With the acceleration of modern social life rhythm and the increase of environmental pressure, the body aging and skin problems caused by oxidative stress are increasingly concerned. Scientific research shows that excessive free radicals will attack cell biological macromolecules, leading to protein denaturation, DNA damage and lipid peroxidation, which not only accelerates the internal aging process of the body, but also is a key factor for causing external aging characteristics such as skin relaxation, wrinkles and color spots. Therefore, by supplementing exogenous antioxidant substances to scavenge free radicals and reduce oxidative damage, it has become one of the effective strategies to delay aging, maintain health and young skin.

[0003] At present, there are various antioxidants for beauty care field, including vitamin C, vitamin E, anthocyanin and the like. However, some synthetic or natural antioxidants have problems such as poor stability, low bioavailability or single function, which limits their application effect. The market urgently needs an active ingredient derived from nature, with clear efficacy, mild effect and multiple anti-aging mechanisms, to develop more efficient and safe beauty care products.

[0004] As a traditional precious Chinese medicinal material, the active ingredient Dendrobium officinale polysaccharide has been confirmed by modern pharmacological research to have excellent antioxidant, immunomodulatory and anti-aging properties. It can significantly improve the activity of antioxidant enzymes such as superoxide dismutase in vivo, directly scavenge various free radicals, and possibly prolong cell lifespan by regulating the signal pathways related to aging. However, the current application research on the composition in the field of beauty care, especially as a core functional factor for scientific compounding and systematic development of high-value antioxidant anti-aging composition, is still insufficient, and its great application potential needs to be further explored and transformed. SUMMARY

[0005] The present application aims to provide an antioxidant anti-aging composition containing Dendrobium officinale polysaccharide and application thereof in beauty care products.

[0006] In order to achieve the above-mentioned application purposes, the present application provides the following technical solutions: The present application provides a preparation method of Dendrobium officinale polysaccharide, comprising the following steps: (1) mixing Dendrobium officinale with water, extracting at 101-104 ℃ for 2-3 h, filtering to obtain a filtrate, and concentrating to obtain a concentrated solution; (2) adding anhydrous ethanol to the concentrated solution to make the ethanol content in the concentrated solution reach 80-85%, and alcohol-sedimenting to obtain a precipitate; (3) Dissolve the precipitate in water to form a precipitate solution, extract it 4 to 6 times with an extractant, and finally take the uppermost liquid to concentrate under reduced pressure and freeze dry to obtain crude polysaccharide; the extractant is chloroform-isoamyl alcohol with a volume ratio of 6 to 8:1. (4) Dissolve the crude polysaccharide in water, load it onto an ion exchange column, elute with ultrapure water, collect the eluent, monitor the sugar content using the phenol-sulfuric acid method, combine fractions with the same absorption peak, concentrate under reduced pressure, dialyze, and freeze dry to obtain the primary polysaccharide product; (5) Dissolve the polysaccharide in water, load it onto a gel column, elute with ultrapure water, collect the eluent, monitor the sugar content using the phenol-sulfuric acid method, combine fractions with the same absorption peak, concentrate under reduced pressure, dialyze, freeze dry, and obtain Dendrobium officinale polysaccharide.

[0007] Preferably, the mass ratio of Dendrobium officinale to water in step (1) is 1:20~25.

[0008] Preferably, the mass ratio of the precipitate to water in step (3) is 1:8~12.

[0009] Preferably, the mass ratio of crude polysaccharide to water in step (4) is 1:8~12.

[0010] Preferably, the ion exchange column in step (4) is a DEAE-Sepharose Fast Flow column.

[0011] Preferably, the gel column in step (5) is a Sephadex G-200 column.

[0012] This invention provides an antioxidant and anti-aging composition containing Dendrobium officinale polysaccharide, comprising the following components in parts by weight: 20-50 parts of Dendrobium officinale polysaccharide, 5-15 parts of grape seed proanthocyanidins, 1-3 parts of Pterocarya stenoptera, 5-15 parts of pine bark extract, 2-5 parts of spermidine, 10-20 parts of Camu camu extract, and 1-3 parts of Piper longum extract.

[0013] This invention provides a method for preparing the aforementioned composition, which involves mixing Dendrobium officinale polysaccharide, grape seed proanthocyanidins (Hangzhou Linran Biotechnology Co., Ltd.), Pterocarya stenoptera (Xi'an Weizhen Biotechnology Co., Ltd.), pine bark extract (Shaanxi Wanyuan Biotechnology Co., Ltd.), spermidine (Shandong Pingju Biotechnology Co., Ltd.), camu camu extract (Shaanxi Wanyuan Biotechnology Co., Ltd.), and Piper longum extract (Shaanxi Wanyuan Biotechnology Co., Ltd.).

[0014] This invention provides the application of the composition in the preparation of beauty and health products with antioxidant and anti-aging effects.

[0015] Compared with the prior art, the present invention has the following beneficial effects: In the antioxidant and anti-aging composition provided by this invention, Dendrobium officinale polysaccharide serves as the core active ingredient, exhibiting a significant synergistic effect with various natural antioxidants such as grape seed proanthocyanidins, pterostilbene, pine bark extract, spermidine, camu camu extract, and Piper longum extract. Dendrobium officinale polysaccharide not only possesses excellent free radical scavenging ability and cell protection function, but also enhances the stability and bioavailability of other components, forming a multi-target, multi-level antioxidant network. For example, grape seed proanthocyanidins and pine bark extract are rich in polyphenols, which can synergistically enhance the scavenging ability against DPPH free radicals, hydroxyl free radicals, and superoxide anions; pterostilbene and spermidine play a synergistic anti-aging role in autophagy and mitochondrial function regulation, further delaying the skin aging process.

[0016] Furthermore, the addition of camu camu extract and long pepper extract further broadens the antioxidant spectrum of the composition, particularly demonstrating a synergistic effect in inhibiting melanin production and reducing photoaging. Experimental data show that this composition significantly outperforms combinations of single ingredients or those lacking certain components in both whitening and antioxidant performance tests, fully validating the synergistic mechanism of the components after compounding. This scientific formulation not only enhances the overall antioxidant capacity but also provides reliable technical support for the development of highly effective, safe, and multi-functional beauty and health products.

[0017] The antioxidant and anti-aging composition containing Dendrobium officinale polysaccharides provided by this invention exhibits multiple beneficial effects through the scientific formulation of its components. Its core beneficial effect lies in achieving highly efficient comprehensive anti-aging function through the synergistic action of multiple targets and pathways. This composition not only powerfully scavenges various free radicals such as DPPH free radicals, hydroxyl free radicals, and superoxide anions, reducing oxidative stress damage, but also effectively inhibits melanin production, demonstrating a clear whitening and spot-fading effect. Experimental data confirms that the complete formula reduces melanin by 7.6%, and performs excellently in all three free radical scavenging tests, especially achieving a scavenging rate of up to 87.5% for hydroxyl free radicals, significantly better than the comparative ratios, reflecting the synergistic advantages of the complete formula.

[0018] This invention, through the scientific compounding of multiple natural active ingredients, not only significantly improves the overall bioavailability of the composition but also effectively ensures the safety and gentleness of the product. In the two-week human skin test in Experiment 1, none of the subjects experienced adverse skin reactions such as allergies, redness, or stinging, fully verifying the high safety of the formula in practical use. This scientific formulation method overcomes the problems of limited efficacy, poor stability, or potential irritation associated with single-ingredient formulations. Attached Figure Description

[0019] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on the provided drawings without creative effort.

[0020] Figure 1 These are the results of the whitening performance test.

[0021] Figure 2 These are the results of the antioxidant test. Detailed Implementation

[0022] The technical solutions provided by the present invention will be described in detail below with reference to the embodiments, but they should not be construed as limiting the scope of protection of the present invention.

[0023] Example 1

[0024] A method for preparing Dendrobium officinale polysaccharide, comprising the following steps: (1) Mix Dendrobium officinale powder and water at a mass ratio of 1:25, extract at 101℃ for 2 hours, filter to obtain filtrate, and concentrate to obtain concentrated solution; (2) Add anhydrous ethanol to the concentrate to make the ethanol content in the concentrate reach 80%, and precipitate by alcohol precipitation. (3) After dissolving the precipitate in water (the mass ratio of precipitate to water is 1:8), a precipitate solution is formed. The solution is extracted four times with an extractant (the volume ratio of precipitate solution to extractant is 5:1). Finally, the uppermost liquid is concentrated under reduced pressure and freeze-dried to obtain crude polysaccharide. The extractant is chloroform-isoamyl alcohol with a volume ratio of 6:1. (4) Dissolve the crude polysaccharide in water (the mass ratio of crude polysaccharide to water is 1:8), load the sample onto the DEAE-Sepharose Fast Flow ion exchange column, elute with ultrapure water, collect the eluent, monitor the sugar content by phenol-sulfuric acid method, combine fractions with the same absorption peak, concentrate under reduced pressure, dialyze with flowing distilled water (dialysis membrane molecular weight cutoff 6000 Da) for 24 h, freeze dry to obtain the primary polysaccharide product; (5) Dissolve the polysaccharide in water, load it onto a Sephadex G-200 gel column, elute with ultrapure water, collect the eluent, monitor the sugar content using the phenol-sulfuric acid method, combine fractions with the same absorption peak, concentrate under reduced pressure, dialyze with flowing distilled water (dialysis membrane molecular weight cutoff 6000 Da) for 24 h, freeze dry, and obtain Dendrobium officinale polysaccharide.

[0025] Example 2

[0026] A method for preparing Dendrobium officinale polysaccharide, comprising the following steps: (1) Mix Dendrobium officinale powder and water at a mass ratio of 1:20, extract at 104℃ for 3 hours, filter to obtain filtrate, and concentrate to obtain concentrated solution; (2) Add anhydrous ethanol to the concentrate to make the ethanol content in the concentrate reach 85%, and precipitate by alcohol precipitation. (3) After dissolving the precipitate in water (the mass ratio of precipitate to water is 1:12), a precipitate solution is formed. The solution is extracted 6 times with an extractant (the volume ratio of precipitate solution to extractant is 7:1). Finally, the uppermost liquid is concentrated under reduced pressure and freeze-dried to obtain crude polysaccharide. The extractant is chloroform-isoamyl alcohol with a volume ratio of 8:1. (4) Dissolve the crude polysaccharide in water (the mass ratio of crude polysaccharide to water is 1:12), load the sample onto the DEAE-Sepharose Fast Flow ion exchange column, elute with ultrapure water, collect the eluent, monitor the sugar content by phenol-sulfuric acid method, combine fractions with the same absorption peak, concentrate under reduced pressure, dialyze with flowing distilled water (dialysis membrane molecular weight cutoff 6000 Da) for 24 h, freeze dry to obtain the primary polysaccharide product; (5) Dissolve the polysaccharide in water, load it onto a Sephadex G-200 gel column, elute with ultrapure water, collect the eluent, monitor the sugar content using the phenol-sulfuric acid method, combine fractions with the same absorption peak, concentrate under reduced pressure, dialyze with flowing distilled water (dialysis membrane molecular weight cutoff 6000 Da) for 24 h, freeze dry, and obtain Dendrobium officinale polysaccharide.

[0027] Example 3

[0028] A method for preparing Dendrobium officinale polysaccharide, comprising the following steps: (1) Mix Dendrobium officinale powder and water at a mass ratio of 1:21, extract at 103℃ for 3 hours, filter to obtain filtrate, and concentrate to obtain concentrated solution; (2) Add anhydrous ethanol to the concentrate to make the ethanol content in the concentrate reach 83%, and precipitate by alcohol precipitation. (3) After dissolving the precipitate in water (the mass ratio of precipitate to water is 1:10), a precipitate solution is formed. The solution is extracted 5 times with an extractant (the volume ratio of precipitate solution to extractant is 6:1). Finally, the uppermost liquid is concentrated under reduced pressure and freeze-dried to obtain crude polysaccharide. The extractant is chloroform-isoamyl alcohol with a volume ratio of 7:1. (4) Dissolve the crude polysaccharide in water (the mass ratio of crude polysaccharide to water is 1:10), load the sample onto the DEAE-Sepharose Fast Flow ion exchange column, elute with ultrapure water, collect the eluent, monitor the sugar content by phenol-sulfuric acid method, combine fractions with the same absorption peak, concentrate under reduced pressure, dialyze with flowing distilled water (dialysis membrane molecular weight cutoff 6000 Da) for 24 h, freeze dry to obtain the primary polysaccharide product; (5) Dissolve the polysaccharide in water, load it onto a Sephadex G-200 gel column, elute with ultrapure water, collect the eluent, monitor the sugar content using the phenol-sulfuric acid method, combine fractions with the same absorption peak, concentrate under reduced pressure, dialyze with flowing distilled water (dialysis membrane molecular weight cutoff 6000 Da) for 24 h, freeze dry, and obtain Dendrobium officinale polysaccharide.

[0029] Example 4

[0030] A method for preparing an antioxidant and anti-aging composition containing Dendrobium officinale polysaccharide, comprising the following steps: by mass ratio, 20 parts of Dendrobium officinale polysaccharide prepared by the method of Example 1, 5 parts of grape seed proanthocyanidins, 1 part of pterostilbene, 5 parts of pine bark extract, 2 parts of spermidine, 10 parts of camu camu extract, and 1 part of Piper longum extract are mixed.

[0031] Example 5

[0032] A method for preparing an antioxidant and anti-aging composition containing Dendrobium officinale polysaccharide, comprising the following steps: 50 parts of Dendrobium officinale polysaccharide prepared in Example 2, 15 parts of grape seed proanthocyanidins, 3 parts of Pterocarya stenoptera, 15 parts of pine bark extract, 5 parts of spermidine, 20 parts of Camu camu extract, and 3 parts of Piper longum extract are mixed by mass ratio.

[0033] Example 6

[0034] A method for preparing an antioxidant and anti-aging composition containing Dendrobium officinale polysaccharide, comprising the following steps: by mass ratio, 35 parts of Dendrobium officinale polysaccharide prepared in Example 3, 10 parts of grape seed proanthocyanidins, 2 parts of pterostilbene, 10 parts of pine bark extract, 3 parts of spermidine, 15 parts of camu camu extract, and 2 parts of Piper longum extract are mixed together.

[0035] Comparative Example 1

[0036] The other methods are the same as in Example 6, except that the extractant in Example 3 is chloroform-n-butanol.

[0037] Comparative Example 2

[0038] The other methods are the same as in Example 6, except that in Example 3, Dendrobium officinale is replaced with an equal amount of Dendrobium nobile.

[0039] Comparative Example 3

[0040] The other methods are the same as in Example 6, except that in Example 6, pterostilbene is replaced with an equal amount of resveratrol.

[0041] Comparative Example 4

[0042] The other methods are the same as in Example 6, except that the addition of pine bark extract and camu camu extract was omitted in Example 6.

[0043] Experiment Example 1: Whitening Performance Test

[0044] 1. Experimental materials: Antioxidant and anti-aging whitening essences prepared in Example 6 and Comparative Examples 1-4.

[0045] Example 6: The preparation method of the essence is as follows: Take a sterilized beaker, add 3.5g of Dendrobium officinale polysaccharide, 1g of grape seed proanthocyanidins, 1g of pine bark extract, 0.3g of spermidine, 1.5g of camu camu extract, and 0.2g of Piper longum extract to 60ml of deionized water, and stir until completely dissolved. The key step is to first dissolve 0.2g of Pterostilbene in 5ml of butylene glycol (or a small amount of alcohol) by preheating, and then slowly add it to the aforementioned aqueous solution while stirring vigorously. Then add 3ml of glycerin and 0.5g of xanthan gum (pre-dispersed in a small amount of water) to thicken, and finally add 1ml of mild cosmetic preservative, and add deionized water to make the total amount 100g. After thorough mixing, pour into a light-proof bottle to prepare an essence with antioxidant and anti-aging effects. The preparation methods of essences in other experimental groups are based on this method.

[0046] 2. Subjects: Fifty healthy women with an average age of 35.3 years were randomly selected. All subjects had no history of skin diseases, the test sites were normal, and they did not apply any cosmetics or drugs unrelated to the experiment in the 15 days before and during the experiment.

[0047] 3. Experimental Methods: The experiment lasted for 2 weeks. All subjects used the designated antioxidant and anti-aging whitening essence once a day. The method of use was as follows: after cleansing and drying the face, apply 2.5g of essence to the entire face. During the test, subjects were prohibited from using any other whitening products. The melanin content of their facial skin was measured using a professional instrument (MEXAMETER MX18 skin pigmentation analyzer). The higher the test value, the higher the melanin content in the skin. All data were finally averaged. The results are shown in Table 1.

[0048] Table 1. Whitening Performance Test Results

[0049] Table 1 shows the melanin reduction rate of Example 6 and each comparative example in the whitening performance test. Example 6 showed the highest melanin reduction rate, reaching 7.6%, indicating its best whitening effect. The melanin reduction rates of Comparative Example 1 (with chloroform-n-butanol as the extractant) and Comparative Example 2 (with Dendrobium officinale replaced by Dendrobium nobile) were 4.4% and 3.7%, respectively, significantly lower than Example 6, indicating that the specific extraction process and raw material selection of Dendrobium officinale polysaccharides have a key impact on the whitening effect. The melanin reduction rate of Comparative Example 3 (with Pterostilbene replaced by resveratrol) was 7.3%, slightly lower than Example 6, but still at a high level, suggesting that Pterostilbene has certain advantages in whitening, but resveratrol can also be used as a substitute ingredient. The whitening effect of Comparative Example 4 (lacking pine bark and camu camu extract) decreased significantly (4.5%), indicating that these two ingredients are indispensable in synergistically enhancing the whitening effect.

[0050] Experiment Example 2 Antioxidant Performance Test

[0051] This experiment used the whitening serums prepared in Example 6 and Comparative Examples 1-4 as test samples to systematically evaluate their in vitro antioxidant properties. The tests included three indicators: DPPH free radicals, hydroxyl free radicals, and superoxide anion free radicals.

[0052] Take the above whitening essence sample solution and the solution with a concentration of 2×10⁻⁶. -4 Add 3 mL of each mol / L DPPH anhydrous ethanol solution to the same stoppered test tube, shake well, and let stand in the dark for 30 min. Measure the absorbance Ai1 of the mixed solution at 517 nm using a UV-Vis spectrophotometer. Simultaneously, measure the absorbance A01 of 3 mL of each extractant mixed with 3 mL of DPPH solution and the absorbance Aj1 of 3 mL of sample solution mixed with 3 mL of anhydrous ethanol. Calculate the clearance rate P1 according to the formula: P1=[1-(Ai1-Aj1) / A01]×100% Take 2 mL of solution with a concentration of 1×10 -2 Add mol / L salicylic acid solution, FeSO4 solution, and diluted sample solution to the same stoppered test tube, then add 2 mL of 8.80 × 10⁻⁶ mol / L salicylic acid solution. -2 A mol / L H₂O₂ solution was shaken well and reacted at 37℃ for 30 min. The absorbance Ai₂ was measured at 510 nm. The absorbance Aj₂ was measured using 2 mL of distilled water instead of the salicylic acid solution. The absorbance A₀₂ was measured using 2 mL of distilled water instead of the sample solution. The clearance rate P₂ was calculated using the formula: P2=[1-(Ai2-Aj2) / A02]×100% Take 5 mL of Tris-HCl buffer solution with pH 8.2 into a stoppered test tube, preheat in a 25°C water bath for 20 min, then add 2 mL of sample solution and 0.5 mL of 5×10⁻⁶ solution sequentially. -3 A mol / L pyrogallol hydrochloric acid solution was prepared, shaken well, and reacted in a 25℃ water bath for 4 min. The absorbance Ai3 was measured at 320 nm. (The last part, "1×10," appears to be a separate, unrelated statement and is left untranslated.) -2 0.5 mL of mol / L HCl solution was used instead of pyrogallol hydrochloric acid solution, and the absorbance Aj3 was measured; 2 mL of distilled water was used instead of the above different sample solutions, and the absorbance A03 was measured. The clearance rate P3 was calculated according to the formula. P3=[1-(Ai3-Aj3) / A03]×100% The experimental results are all average values, as shown in Table 2.

[0053] Table 2 Antioxidant Test Results

[0054] Table 2 shows the antioxidant performance of each experimental group in the three free radical scavenging experiments. Example 6 showed the best performance in DPPH, hydroxyl radical, and superoxide anion radical scavenging rates, at 66.2%, 87.5%, and 76.8%, respectively, demonstrating comprehensive and efficient antioxidant capacity. The antioxidant capacity of Comparative Examples 1 and 2 decreased significantly, especially in hydroxyl radical scavenging, further confirming the importance of the extraction process and raw materials of Dendrobium officinale polysaccharide for its antioxidant activity. Although the antioxidant performance of Comparative Example 3 was slightly lower than that of Example 6, it was still better than the other comparative examples, indicating that Pterostilbene has a positive role in antioxidant activity, but resveratrol also has certain substitution potential. The antioxidant capacity of Comparative Example 4 decreased significantly, especially in the superoxide anion scavenging rate, which was only 54.3%, significantly lower than the other groups, highlighting the key role of pine bark extract and camu camu extract in improving the overall antioxidant performance of the composition.

[0055] The data from Tables 1 and 2 show that the composition represented by Example 6 exhibits optimal effects in both whitening and anti-oxidation. The key to its success lies in the highly efficient extraction process of Dendrobium officinale polysaccharides, the synergistic effect of Pterocarya stenoptera, and the addition of pine bark extract and Camu camu extract, which together construct a multi-target, multi-mechanism anti-aging system. The decreased efficacy in each comparative example also indirectly verifies the necessity and synergy of these ingredients and processes.

[0056] The above description is only a preferred embodiment of the present invention. It should be noted that for those skilled in the art, several improvements and modifications can be made without departing from the principle of the present invention, and these improvements and modifications should also be considered within the scope of protection of the present invention.

Claims

1. A method for preparing Dendrobium officinale polysaccharide, characterized in that, Includes the following steps: (1) Mix Dendrobium officinale with water, extract at 101~104℃ for 2~3h, filter to obtain filtrate, concentrate to obtain concentrated solution; (2) Add anhydrous ethanol to the concentrate to make the ethanol content in the concentrate reach 80-85%, and precipitate the ethanol to obtain a precipitate; (3) Dissolve the precipitate in water to form a precipitate solution, extract it 4 to 6 times with an extractant, and finally take the uppermost liquid to concentrate under reduced pressure and freeze dry to obtain crude polysaccharide; the extractant is chloroform-isoamyl alcohol with a volume ratio of 6 to 8:

1. (4) Dissolve the crude polysaccharide in water, load it onto an ion exchange column, elute with ultrapure water, collect the eluent, monitor the sugar content using the phenol-sulfuric acid method, combine fractions with the same absorption peak, concentrate under reduced pressure, dialyze, and freeze dry to obtain the primary polysaccharide product; (5) Dissolve the polysaccharide in water, load it onto a gel column, elute with ultrapure water, collect the eluent, monitor the sugar content using the phenol-sulfuric acid method, combine fractions with the same absorption peak, concentrate under reduced pressure, dialyze, freeze dry, and obtain Dendrobium officinale polysaccharide.

2. The method for preparing Dendrobium officinale polysaccharide according to claim 1, characterized in that, The mass ratio of Dendrobium officinale to water in step (1) is 1:20~25.

3. The method for preparing Dendrobium officinale polysaccharide according to claim 1, characterized in that, The mass ratio of the precipitate to water in step (3) is 1:8~12.

4. The method for preparing Dendrobium officinale polysaccharide according to claim 1, characterized in that, The mass ratio of crude polysaccharide to water in step (4) is 1:8~12.

5. The method for preparing Dendrobium officinale polysaccharide according to claim 1, characterized in that, The ion exchange column in step (4) is a DEAE-Sepharose Fast Flow column.

6. The method for preparing Dendrobium officinale polysaccharide according to claim 1, characterized in that, The gel column in step (5) is a Sephadex G-200 column.

7. An antioxidant and anti-aging composition containing Dendrobium officinale polysaccharides, characterized in that, The product comprises the following components in parts by weight: 20-50 parts of Dendrobium officinale polysaccharide prepared by the method according to any one of claims 1 to 6, 5-15 parts of grape seed proanthocyanidins, 1-3 parts of Pterocarya stenoptera, 5-15 parts of pine bark extract, 2-5 parts of spermidine, 10-20 parts of Camu camu extract, and 1-3 parts of Piper longum extract.

8. The method for preparing the composition according to claim 7, characterized in that, Simply mix Dendrobium officinale polysaccharide, grape seed proanthocyanidins, Pterospermum erythrorhizon, pine bark extract, spermidine, camu camu extract, and Piper longum extract.

9. The use of the composition according to claim 7 in the preparation of beauty and health products with antioxidant and anti-aging effects.

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