Moraxella roselle MT01 and application thereof in CO2 fixation and / or sediment carbon emission reduction

Abstract

The invention discloses Moraxella roselle MT01 and application thereof in CO2 fixation and / or sediment carbon emission reduction, and belongs to the technical field of microorganisms. According to the invention, a strain of Moraxella roselle MT01 is obtained through screening and identification, and the preservation number is CCTCC (China Center For Type Culture Collection) NO: M 20252028. Through verification, the strain MT01 contains two key carbon sequestration genes, namely fhs and cbbM; whether the strain MT01 has the carbon sequestration capability or not is detected by using isotope, and the result shows that delta 13C exceeds 60 per thousand; the carbon sequestration capability of the strain MT01 is detected by adopting sediment carbon emission reduction, the result shows that the strain MT01 can obviously reduce the emission of CO2 in mangrove forest sediment, and the emission reduction efficiency is about 16.9%. The invention provides new strain selection and technical basis for microbial carbon sequestration.

Description

Technical Field

[0001] This invention relates to the field of microbial technology, and in particular to a strain of Moraxella roselliae MT01 and its application in CO2 fixation and / or sediment carbon reduction. Background Technology

[0002] Human activities have profoundly impacted the Earth's carbon cycle, disrupting the balance of global carbon sinks and leading to climate change, extreme weather events, and ecological imbalances. A stable carbon cycle is crucial for ensuring a healthy biosphere. To rapidly restore this balance, emission reduction and increasing carbon sinks are two fundamental strategies. Microorganisms are an indispensable and vital component of life on Earth, widely distributed in various ecological environments, from land and sea to extreme conditions, demonstrating remarkable vitality and adaptability. Microorganisms make significant contributions to the global carbon cycle. It is estimated that approximately 50-60% of global organic carbon fixation annually is driven by microorganisms. Through a series of complex biochemical reactions, these microorganisms convert atmospheric carbon dioxide into organic matter, providing energy and precursors for their own growth and effectively fixing large amounts of greenhouse gases within their bodies or in environments such as soil and sediments, thus slowing the rate of global warming. Therefore, isolating and purifying microorganisms with unique and efficient carbon fixation capabilities is of great significance for future exploration of marine carbon sink potential.

[0003] Carbon-fixing microorganisms are widely distributed and diverse in nature. Autotrophic microorganisms primarily utilize CO2 for photosynthesis or chemosynthesis to fix CO2 from the environment. Based on their energy source, they are further divided into photoautotrophic and chemoautotrophic microorganisms. Carbon-fixing microorganisms can also be classified according to whether they require light into photocarbon-fixing microorganisms and dark carbon-fixing microorganisms. Photocarbon-fixing microorganisms, also known as photoautotrophic microorganisms, mainly include microalgae and photosynthetic bacteria. Dark carbon-fixing microorganisms encompass chemoautotrophic and heterotrophic microorganisms, such as nitrifying bacteria, hydrogen bacteria, sulfur bacteria, and iron bacteria.

[0004] Microbial carbon fixation strategies are diverse, primarily involving: the Calvin cycle, the reductive TCA cycle, the anaerobic acetyl-CoA pathway, the 3-hydroxypropionate cycle, the 3-hydroxypropionate / 4-hydroxybutyrate cycle, the dicarboxylate / 4-hydroxybutyrate cycle, and the reverse glycinecleavage pathways. These pathways exhibit unique characteristics in different ecological environments. The Calvin cycle is widely found on the Earth's surface where oxygen is abundant and sunlight is plentiful. In contrast, the 3-hydroxypropionate bicycle and the 3-hydroxypropionate / 4-hydroxybutyrate pathways mainly occur under anaerobic or hypoxic conditions. The reduced tricarboxylic acid cycle primarily operates in anaerobic environments, such as freshwater lakes and the euphotic zone in the deep sea, mainly due to the environmental adaptability of its functional enzymes. The reduced acetyl-CoA pathway and the dicarboxylic acid / 4-hydroxybutyrate cycle pathway are primarily found in anaerobic environments, currently discovered in deep-sea sediments or deep-sea hydrothermal vents. Previous studies have often focused on single carbon fixation pathways in microorganisms, with limited research on microorganisms with multiple carbon fixation pathways.

[0005] Marine sediments are a vital global carbon sink, with organic carbon storage far exceeding that of terrestrial soils. Current technologies for enhancing marine carbon sequestration primarily fall into two categories: physicochemical methods, such as seabed carbonate mineral sequestration and artificial upwelling pumping of nutrients. These technologies are energy-intensive, costly, and may disrupt the marine ecological balance. Bioaugmentation methods utilize phytoplankton (such as diatoms) or cyanobacteria for photosynthetic carbon sequestration. However, these microorganisms rely on sunlight and cannot function effectively in light-deprived environments like deep-sea sediments, and their carbon sequestration pathway is singular (primarily the Calvin cycle).

[0006] Existing research indicates the presence of naturally occurring carbon-fixing microorganisms (such as sulfate-reducing bacteria and methanogens) in sediments, but their carbon fixation capabilities suffer from significant drawbacks: Limited pathways: Most strains rely on only one or two carbon fixation pathways (such as the reductive tricarboxylic acid cycle or the Wood-Ljungdahl pathway), resulting in poor environmental adaptability. Low efficiency: Carbon fixation rates in deep-sea sediments are generally below 10 μmol C / g / day and are easily limited by electron donors (such as H2 and organic acids). Insufficient carbon stability: Carbon assimilated by microorganisms is often released again due to mineralization by heterotrophic bacteria, resulting in a long-term sediment sequestration rate of less than 40%. Therefore, there is an urgent need to develop a carbon-fixing strain to provide a technological foundation for addressing these problems. Summary of the Invention

[0007] The purpose of this invention is to provide a strain of *Moraxella rosenbergii* MT01 and its application in CO2 fixation and / or sediment carbon reduction, in order to solve the problems existing in the prior art. Strain MT01 can significantly reduce CO2 emissions from mangrove sediments, with a reduction efficiency of approximately 16.9%. This invention provides a new strain selection and technical basis for microbial carbon fixation.

[0008] To achieve the above objectives, the present invention provides the following solution:

[0009] This invention provides a strain of Rossellomorea sp. MT01, which was deposited on September 15, 2025 at the China Center for Type Culture Collection (CCTCC) with accession number CCTCC NO: M20252028, and the deposit address is Wuhan University, Wuhan, China.

[0010] The present invention also provides the application of the aforementioned Rosellium MT01 in the preparation of microbial inoculants.

[0011] The present invention also provides a microbial agent comprising the aforementioned *Roseola molaris* MT01.

[0012] Optionally, the bacterial concentration of *Moraxella rosenbergii* MT01 is 1.3 × 10⁻⁶. 6 cells / mL.

[0013] The present invention also provides the application of the aforementioned *Roseolomewia MT01* or the aforementioned microbial agent in CO2 fixation and / or sediment carbon reduction.

[0014] The present invention also provides the application of the aforementioned *Roseolomewia MT01* or the aforementioned microbial agent in the preparation of products for CO2 fixation and / or sediment carbon reduction.

[0015] Optionally, the bacterial concentration of *Moraxella rosenbergii* MT01 is 1.3 × 10⁻⁶. 6 cells / mL.

[0016] The present invention also provides a product for fixing CO2 and / or reducing carbon emissions from sediments, the product comprising the aforementioned *Roseola molluscum MT01*.

[0017] Optionally, the bacterial concentration of *Moraxella rosenbergii* MT01 is 1.3 × 10⁻⁶. 6 cells / mL.

[0018] The present invention discloses the following technical effects:

[0019] This invention screened and identified a carbon-fixing strain, specifically *Rosellomoreasp.*, named MT01. This strain was deposited on September 15, 2025, at the China Center for Type Culture Collection (CCTCC), accession number CCTCC NO: M 20252028, located at Wuhan University, Wuhan, China. Verification showed that strain MT01 contains two key carbon-fixing genes, fhs and cbbM. Isotope analysis was used to determine whether strain MT01 possesses carbon-fixing ability, and the results showed that… 13 The carbon content (C) exceeded 600‰. Subsequent sediment carbon reduction assays were used to assess the carbon sequestration capacity of strain MT01. The results showed that strain MT01 significantly reduced CO2 emissions from mangrove sediments, with a reduction efficiency of approximately 16.9%. This invention provides a new strain selection and technological basis for microbial carbon sequestration. Attached Figure Description

[0020] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the drawings used in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0021] Figure 1 This is a colony diagram of strain MT01;

[0022] Figure 2 Gram staining image of strain MT01;

[0023] Figure 3 Electron micrograph of strain MT01;

[0024] Figure 4 Phylogenetic tree of MT01 strain;

[0025] Figure 5 The image shows the PCR amplification diagrams of the fhs(a) and cbbM(b) genes of strain MT01.

[0026] Figure 6 Identification of carbon fixation in strain MT01 13 (C isotope labeling);

[0027] Figure 7 Photographs of a laboratory simulation of a sedimentary environment;

[0028] Figure 8 The changes in CH4 (a), CO2 (b), and N2O (c) emissions after the MT01 strain was sealed with mangrove sediments for 24 h. Detailed Implementation

[0029] Various exemplary embodiments of the present invention will now be described in detail. This detailed description should not be considered as a limitation of the present invention, but rather as a more detailed description of certain aspects, features, and embodiments of the present invention.

[0030] It should be understood that the terminology used in this invention is merely for describing particular embodiments and is not intended to limit the invention. Furthermore, with respect to numerical ranges in this invention, it should be understood that each intermediate value between the upper and lower limits of the range is also specifically disclosed. Any stated value or intermediate value within a stated range, as well as each smaller range between any other stated value or intermediate value within said range, is also included within the scope of this invention. The upper and lower limits of these smaller ranges may be independently included or excluded from the range.

[0031] Unless otherwise stated, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art. While only preferred methods and materials have been described herein, any methods and materials similar or equivalent to those described herein may be used in the implementation or testing of this invention. All references to this specification are incorporated by way of citation to disclose and describe methods and / or materials associated with those references. In the event of any conflict with any incorporated reference, the content of this specification shall prevail.

[0032] Various modifications and variations can be made to the specific embodiments described in this specification without departing from the scope or spirit of the invention, as will be apparent to those skilled in the art. Other embodiments derived from this specification will also be obvious to those skilled in the art. This specification and embodiments are merely exemplary.

[0033] The terms “include,” “including,” “have,” “contain,” etc., used in this article are all open-ended terms, meaning that they include but are not limited to.

[0034] Carbon-free solid culture medium: Artificial seawater is prepared by adding 100 g NaCl, 2.5 g MgSO4·H2O, 1.5 g NH4Cl, 1.5 g KCl, 1.5 g K2HPO4, and 0.19 g CaCl2 to every 5 L of sterile water. Carbon-free solid culture medium is prepared by adding 1.7 g agar to every 100 mL of artificial seawater. The carbon-free liquid culture medium is the aforementioned artificial seawater.

[0035] Example 1 Isolation and Identification of Strains

[0036] 1. Sediment sample collection

[0037] Based on an ecological survey of the thick-shelled mussel farming area in Shengsi, Zhejiang (30°42′N, 122°46′E), in August 2021, the research group used a cable grab bucket (QNC type, 0.025 m) to...2 Sediment samples were collected (at a water depth of approximately 15 m). The top 5 cm of sediment was placed in a sterile sampling bottle, and after labeling the relevant information, the samples were returned to the laboratory for the isolation and purification of carbon-fixing strains.

[0038] 2. Bacterial Isolation Methods

[0039] Take 3 g of sediment sample and place it in 27 mL of sterilized artificial seawater. Shake the sample on a constant-temperature shaker for 20 h (150 r / min). Then, under sterile conditions, transfer the turbid liquid to a container at a concentration of 10... -3 10 -4 10 -5 10 -6 Perform isochronous dilutions, and spread 0.2 mL of the sample suspension onto sterile carbon-free culture plates. Incubate at 25°C inverted position. Isolate the colonies that grow on the plates, and repeatedly streak them for purification until a single strain is obtained (using the same sterile carbon-free culture plates as described above). Gram stain the single strain.

[0040] The MT01 strain was isolated from carbon-free culture plates by streak plating. This strain grows relatively slowly, with flocculent edges at the colony margins, a convex center, and an orange-pink surface. Figure 1 Gram staining was negative. Figure 2 The strain is short rod-shaped ( Figure 3 ).

[0041] 3. Bacterial DNA extraction

[0042] This embodiment uses a bacterial genomic DNA extraction kit (TIANGEN, bp302) to extract, isolate, and purify DNA from strain MT01. The specific method is as follows: Take 5 mL of the cultured bacterial sample and centrifuge at 10000 rpm for 1 min, removing as much supernatant as possible. Add 200 μL of buffer GA to the precipitated bacterial cells, ensuring complete resuscitation, then add 20 μL of proteinase K and mix thoroughly. Next, add 220 μL of buffer GB, gently shake for 15 s, and heat in a 70°C water bath for 10 min until the solution becomes clear. Then, briefly centrifuge to remove water droplets from the tube opening. Add 220 μL of anhydrous ethanol, mix again for 15 s, and then briefly centrifuge again to remove water droplets. Pour the treated solution and precipitate together into a DNA adsorption column, centrifuge at 12000 rpm for 30 s, discard the supernatant, and then place the adsorption column into a collection tube. Add 500 μL of GD buffer to the adsorption column, repeat the centrifugation steps described above, discard the liquid, and then place the adsorption column into the collection tube. Add 600 μL of PW wash buffer to the adsorption column, centrifuge at 12000 rpm for 30 s, discard the supernatant, and place the adsorption column into the collection tube. Repeat the previous step. Place the adsorption column back into the collection tube, centrifuge at 12000 rpm for 2 min, discard the supernatant, and allow the column to air dry at room temperature to remove any residual wash buffer. Finally, transfer the adsorption column to a clean centrifuge tube, add 50 to 200 μL of TE elution buffer to the central region, incubate at room temperature for 2-5 min, then centrifuge at 12000 rpm for 2 min. Collect the solution and store it at -20°C for subsequent analysis.

[0043] 4. Molecular identification of strains

[0044] The 16S rRNA gene of strain MT01 was amplified.

[0045] The PCR reaction was performed in a 25 µL system (Shanghai Sangon Biotech): 1 µL DNA template, 1 µL primer 27F (10 mM), 1 µL primer 1492R (10 mM), 12.5 µL 2×PCR mix, and 9.5 µL ddH2O. Primer 27F: 5′-AGTTTGATCCTGGCTCA-3′ (SEQ ID NO.1), primer 1492R: 5′-TACCTTGTTACGACTTCA-3′ (SEQ ID NO.2).

[0046] PCR reaction procedure: pre-denaturation at 94℃ for 3 min; followed by 35 cycles, including 94℃ for 1 min, 50℃ for 1 min, and 72℃ for 3 min; and finally, a final extension at 72℃ for 10 min.

[0047] PCR products were detected by 1.0% agarose gel electrophoresis (120 V for 30 min), followed by observation and photography using a gel imaging system. The PCR products were sent to Sangon Biotech (Shanghai) Co., Ltd. for sequencing. The obtained sequences were submitted to NCBI for BLASTn alignment (http: / / www.ncbi.nlm.nih.gov / blast / ) to search for similar sequences, and homology analysis results were obtained after alignment. Highly homologous genes and outgroup genes were searched in the NCBI database, downloaded, and combined with the sequenced genes for ClustalW alignment in MEGA11. The Neighbor-Joining algorithm was used, and a phylogenetic tree was obtained through 1000 Bootstrap iterations.

[0048] After identification by 16S rRNA sequence, such as Figure 4 As shown, this strain has a very high similarity (99.89%) to Rossellomorea aquimaris. Therefore, strain MT01 was identified as Rossellomorea sp. and named MT01. This strain was deposited at the China Center for Type Culture Collection on September 15, 2025, with accession number CCTCC NO: M 20252028, and the deposit address is Wuhan University, Wuhan, China.

[0049] Example 2 Identification of carbon fixation function of strain MT01

[0050] 1. Identification of carbon fixation genes in strain MT01

[0051] The total DNA extracted from the MT01 strain was used as a template to amplify the carbon fixation functional genes cbbM and fhs.

[0052] cbbM upstream primer: 5′-CCATGGTGCCTACGTGAATACC-3′ (SEQ ID NO.3), downstream primer: 5′-GTGGTGACTTCATCAAGAATGATGA-3′ (SEQ ID NO.4); fhs upstream primer: 5′-CGTAGGTGCCCATCGTAATACC-3′ (SEQ ID NO.5), downstream primer: 5′-ATGCGTGACTTCATCAAGAATGATGA-3′ (SEQ ID NO.6).

[0053] The PCR reaction used a 20 μL system: 2 μL DNA template, 0.5 μL upstream primer, 0.5 μL downstream primer, 10 μL 2×Taq PCR mix, and 8 μL ddH2O. The PCR reaction program was as follows: 94℃ for 3 min, followed by 35 cycles, including 94℃ denaturation for 30 s, 56℃ annealing for 30 s, 72℃ extension for 45 s, and a final extension at 72℃ for 5 min. The PCR products were confirmed by 1% agarose gel electrophoresis, and the target band PCR product was excised and recovered. The recovered PCR products were cloned and ligated. The positive recombinant plasmid was sent for assaying. The sequenced sequences were submitted to NCBI for BLASTn alignment, searching for similar sequences. After downloading the relevant sequences, a phylogenetic tree was constructed and analyzed.

[0054] Results: The MT01 strain contained two key carbon fixation genes, fhs and cbbM. Figure 5 ).

[0055] fhs is the functional gene for formyltetrahydrofolate synthase (FTFHS), a key enzyme in the WL pathway (reduced acetyl-CoA pathway). cbbM is the functional gene for ribulose-1,2-bisphosphate carboxylase, a key enzyme in the Calvin cycle pathway. The Calvin cycle carbon fixation pathway mainly exists in aerobic environments with light conditions and is the main carbon fixation pathway for plants, photosynthetic microorganisms, and some dark carbon-fixing microorganisms, playing a significant role in regulating carbon dioxide concentration and even the global carbon cycle.

[0056] 2. Identification of carbon fixation capacity of strain MT01 (isotope detection)

[0057] LB liquid culture medium formula (1 L): 10 g tryptone, 5 g yeast extract and 10 g sodium chloride.

[0058] Formula for oligotrophic artificial seawater culture medium (1 L) containing 0.05 g peptone: 20 g sodium chloride, 0.434 g magnesium sulfate, 0.3 g dipotassium hydrogen phosphate, 0.04 g calcium chloride, 0.3 g potassium chloride and 0.5 g peptone.

[0059] Formula for artificial seaweed jelly liquid culture medium (1 L) containing 0.2 g / 100 mL agar: 20 g sodium chloride, 0.434 g magnesium sulfate, 0.3 g dipotassium hydrogen phosphate, 0.04 g calcium chloride, 0.3 g potassium chloride and 2 g agar powder.

[0060] After autoclaving, 0.04 g / 100 mL of filtered and sterilized stable isotope was added. 13 C(NaHCO3). A negative control was prepared using a culture medium without isotope labeling.12 C-labeled medium). Strain MT01 was cultured in the above three culture media (25℃ shaker culture), with *Pseudomonas aeruginosa* as the control group. Periodically, 200 mL of bacterial suspension was taken, centrifuged at 10000 rpm for 10 min, the supernatant was removed, and the bacterial cells were retained. The cells were then freeze-dried and sent for analysis.

[0061] The results are as follows Figure 6 As shown, compared with the non-carbon-fixing bacterium *Pseudomonas aeruginosa*, MT01 exhibits a certain carbon fixation capacity, δ 13 C exceeds 600‰.

[0062] Example 3 Identification of carbon fixation ability of strain MT01

[0063] The carbon fixation capacity of strain MT01 was tested using sediment carbon reduction assay.

[0064] Mangrove sediment pretreatment: After the sediment is air-dried, the tree roots are removed and it is stored in a refrigerator at 4°C for later use.

[0065] Liquid culture of strain MT01 and Pseudomonas aeruginosa: Strain MT01 was cultured in carbon-free liquid medium supplemented with 1 g peptone per liter of medium. Pseudomonas aeruginosa was cultured in LB medium.

[0066] Before the experiment, the OD value of the bacterial culture was measured to be 0.4. The bacterial count was determined using a cell counter, with strain MT01 having a count of 1.3 × 10⁻⁶. 6 The number of cells / mL was 3.4 × 10⁻⁶ for Pseudomonas aeruginosa. 7 cells / mL. Take 2.7 L of MT01 bacterial culture and 2.7 L of Pseudomonas aeruginosa bacterial culture, respectively, and centrifuge at 12000 rpm for 5 min to separate the bacterial cells. After centrifugation, remove the supernatant and retain the bacterial cells in each centrifuge tube (each centrifuge tube contains 450 mL of bacterial culture, with six centrifuge tubes for MT01 and six for Pseudomonas aeruginosa), and process them as follows.

[0067] Treatment 1: 250 g of sediment was thoroughly mixed with 225 mL of sterilized artificial seawater (salinity 28‰), and the mixture was added to three blue-capped bottles, each containing 90 g of sediment. Figure 7 ).

[0068] Treatment 2: All centrifuge tubes containing MT01 bacteria were resuspended using 225 mL of sterilized artificial seawater (salinity 28‰), and then mixed thoroughly with 250 g of sediment before being added to three blue-capped bottles, 90 g in each bottle.

[0069] Treatment 3: All centrifuge tubes containing Pseudomonas aeruginosa were resuspended using 225 mL of sterilized artificial seawater (salinity 28‰), and then mixed thoroughly with 250 g of sediment before being added to three blue-capped bottles, 90 g in each bottle.

[0070] Gas collection: 24 hours after the experimental setup was completed, the bottle was capped. A vacuum process was performed 12 hours later, with two bags (50 mL) of gas removed from each bottle. After standing for 12 hours, the bottle was capped again. Before each capping, a fan was used to blow air through the bottle opening to ensure the air inside and outside the bottle was consistent. This cycle was repeated for seven days, with a total of three vacuum processes.

[0071] The results are as follows Figure 8 As shown, MT01 can significantly reduce CO2 emissions from mangrove sediments with an efficiency of approximately 16.9%, but has no significant effect on greenhouse gases such as CH4 and N2O.

[0072] In summary, the experimental results indicate that strain MT01 possesses multiple carbon fixation pathways and the potential to fix carbon under varying oxygen concentrations, demonstrating a significant role in reducing sediment carbon emissions. Therefore, future research will further explore the ecological mechanisms of this strain in complex environments, conduct molecular modifications to unlock its carbon fixation potential, and investigate the synergistic relationships between this carbon-fixing microorganism and environmental microorganisms. By precisely regulating the expression levels of key carbon-fixing enzyme genes and optimizing each step in the carbon fixation pathway, a highly efficient carbon-fixing microorganism can be constructed, potentially creating a novel carbon fixation system. Furthermore, there is significant room for research into the interaction networks of multi-pathway carbon-fixing bacteria and their roles at the ecosystem level.

[0073] The embodiments described above are merely preferred embodiments of the present invention and are not intended to limit the scope of the present invention. Various modifications and improvements made by those skilled in the art to the technical solutions of the present invention without departing from the spirit of the present invention should fall within the protection scope defined by the claims of the present invention.

Claims

1. A strain of Rossell morea sp. MT01, characterized in that, The Moraxella rossica MT01 has been deposited with the China Center for Type Culture Collection on September 15, 2025, and the deposit number is CCTCC NO: M20252028, and the deposit address is Wuhan, China, Wuhan University.

2. Use of the Moraxella rossica MT01 of claim 1 in the preparation of a microbial inoculant.

3. A microbial inoculant, characterized in that, The microbial inoculant comprises the Moraxella rossica MT01 of claim 1.

4. The microbial inoculant of claim 3, wherein, The bacterial concentration of the Moraxella rossica MT01 was 1.3 x 10 6 cells / mL.

5. Use of the Moraxella rossica MT01 of claim 1 or the microbial inoculant of claim 3 in the fixation of CO2 and / or sediment carbon emission reduction.

6. Use of the Moraxella rossica MT01 of claim 1 or the microbial inoculant of claim 3 in the preparation of a product for the fixation of CO2 and / or sediment carbon emission reduction.

7. Use according to claim 5 or 6, wherein the compound is ###0002### The bacterial concentration of the Moraxella rossica MT01 was 1.3 x 10 6 cells / mL.

8. A product for sequestering CO2 and / or sediment carbon emission reduction, characterized in that, The product comprises the Moraxella rossica MT01 of claim 1.

9. The product of claim 8, wherein, The bacterial concentration of the Moraxella rossica MT01 was 1.3 x 10 6 cells / mL.

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