Pesticide composition and application thereof

Abstract

The invention belongs to the field of pesticides, and relates to a pesticide composition and application thereof. The pesticide composition comprises an active component A and an active component B, the active component A is a compound represented by a formula (I), the active component B is any one of flupyroxystrobin and bentioflumin, and the mass ratio of the active component A to the active component B is 1: 27-35: 1. The composition disclosed by the invention has the advantages of synergism, resistance reduction, safety to crops and the like, and can be used for preventing and controlling agricultural and forestry pests and sanitary pests.

Description

Technical Field

[0001] This invention belongs to the field of pesticide technology, specifically relating to a pesticide composition and its application. Background Technology

[0002] Bentioflumin is a methoxyacrylate insecticide with CAS number 2566451-67-8 and chemical name methyl 2-({4-chloro-2-fluoro-5-[(2,2,2-trifluoroethyl)thioalkyl]phenylamino}methyl)benzoate. Its chemical structural formula is as follows:

[0003]

[0004] Flupyroxystrobin is a methoxyacrylate insecticide with CAS number 114077-75-7. Its chemical structural formula is as follows:

[0005]

[0006] In agricultural production, the control of various harmful insects mainly relies on the application of large quantities of pesticides. The excessive and frequent irrational use of pesticides leads to increasing resistance in harmful insects, causing more severe damage to crops and making control increasingly difficult. Combining two or more pesticides with different mechanisms of action can achieve simultaneous control of different pests, improve pesticide efficacy, and reduce the development of pesticide resistance. The applicant, through indoor toxicity experiments and field efficacy trials, surprisingly discovered that the combination of compound (I) with flupyroxystrobin and bentioflumin has a significant synergistic effect. Furthermore, the acaricide composition and application of compound (I) combined with flupyroxystrobin and bentioflumin have not been reported before. Summary of the Invention

[0007] The purpose of this invention is to provide a pesticide composition that has the effects of synergistic effect, reducing resistance, and is safe for crops.

[0008] To achieve the above objectives, the technical solution adopted by the present invention is as follows: a pesticide composition, wherein the pesticide composition comprises active ingredient A and active ingredient B, wherein active ingredient A is a compound of formula (I), and its chemical structural formula is:

[0009] Its chemical structural formula is: The active ingredient B is either flupyroxystrobin or bentioflumin;

[0010] Furthermore, the mass ratio between active ingredient A and active ingredient B is 1:27 to 35:1;

[0011] Furthermore, the mass ratio of the compound of formula (I) to flupyroxystrobin is 1:20 to 25:1 or any value between the above values;

[0012] Furthermore, the mass ratio of the compound of formula (I) to flupyroxystrobin is 1:20, 1:7, 1:4, 1:2, 3:2, 12:1, or 25:1;

[0013] Furthermore, the mass ratio of the compound of formula (I) to bentioflumin is 1:27 to 35:1;

[0014] Furthermore, the mass ratio of the compound of formula (I) to bentioflumin is 1:20 to 23:1 or any value between the above values;

[0015] Furthermore, the mass ratio of the compound of formula (I) to bentioflumin is 1:20, 1:5, 9:1, 12:1, 16:1, or 23:1;

[0016] Furthermore, based on a total mass of 100 wt% of the pesticide composition, the sum of the contents of active ingredient A and active ingredient B in the pesticide composition is 0.5% to 90%.

[0017] Furthermore, based on a total mass of 100 wt% of the pesticide composition, the sum of the contents of active ingredient A and active ingredient B in the pesticide composition is 10% to 60%.

[0018] Furthermore, based on a total mass of 100 wt% of the pesticide composition, the sum of the contents of active ingredient A and active ingredient B in the pesticide composition is 20%, 25%, 30%, 35%, 40%, 45%, 52%, and 60%, respectively.

[0019] Furthermore, the pesticide composition further includes an adjuvant, which includes a carrier and an adjuvant;

[0020] Furthermore, the carrier is one or more of water, solvent, or filler;

[0021] Furthermore, the carrier is deionized water;

[0022] Further, the solvent is selected from one or more of the following: N,N-dimethylformamide, cyclohexanone, toluene, xylene, dimethyl sulfoxide, methanol, ethanol, trimethylcyclohexanone, N-octylpyrrolidone, ethanolamine, triethanolamine, isopropylamine, N-methylpyrrolidone, propanol, butanol, ethylene glycol, diethylene glycol, ethylene glycol methyl ether, butyl ether, ethanolamine, isopropylamine, ethyl acetate, or acetonitrile.

[0023] Furthermore, the filler is selected from one or more of the following: kaolin, diatomaceous earth, bentonite, attapulgite, silica, starch, or light calcium carbonate.

[0024] The additives include at least one surfactant, and may also include other functional additives such as antifreeze, thickener, stabilizer, disintegrant, defoamer, slow-release agent, and binder, depending on the application and requirements.

[0025] The surfactant is selected from one or more of emulsifiers, dispersants, wetting agents, dispersion media, or penetrants;

[0026] The surfactants mentioned above are common nonionic or anionic surfactants, either as single agents or in combination; the other functional additives are selected from one or more of antifreeze agents, thickeners, stabilizers, disintegrants, or defoamers.

[0027] Furthermore, the emulsifier is selected from one or a mixture of multiple of the following: agricultural emulsion 500# (calcium alkylbenzene sulfonate), OP series phosphate esters (surfactant phosphate esters), 600# phosphate esters (phenylphenol polyoxyethylene ether phosphate esters), styrene polyoxyethylene ether wetting salts, alkyl biphenyl ether magnesium disulfonate salts, triethanolamine salts, agricultural emulsion 400# (benzyl dimethylphenol polyoxyethylene ether), agricultural emulsion 700# (alkylphenol formaldehyde resin polyoxyethylene ether), Ningxia emulsion 36# (phenylethylphenol formaldehyde resin polyoxyethylene ether), agricultural emulsion 1600# (phenylethylphenol polyoxyethylene polypropylene ether), ethylene oxide-propylene oxide block copolymers, OP series (surfactants), BY series (castor oil polyoxyethylene ether), agricultural emulsion 33# (alkyl aryl polyoxyethylene polyoxypropylene ether), Span series (sorbitan monostearate), Tween series (dehydrated sorbitan fatty acid ester polyoxyethylene ether), or AEO series (fatty alcohol polyoxyethylene ether).

[0028] Furthermore, the dispersant is selected from one or more of the following: polycarboxylate, lignin sulfonate, alkylphenol polyoxyethylene ether formaldehyde condensate sulfate, calcium alkylbenzene sulfonate, sodium naphthalene sulfonate formaldehyde condensate, alkylphenol polyoxyethylene ether, fatty amine polyoxyethylene ether, fatty acid polyoxyethylene ether, or glycerol fatty acid ester polyoxyethylene ether.

[0029] Furthermore, the wetting agent is selected from one or more of the following: sodium dodecyl sulfate, sodium dodecylbenzene sulfonate, pull-opening powder BX, wetting and penetrating agent F, soapberry powder, silkworm excrement, or soapberry powder;

[0030] Furthermore, the penetrant is selected from one or more of the following: penetrant JFC (fatty alcohol polyoxyethylene ether), penetrant T (diisooctyl maleate sulfonate), azone, or organosilicon;

[0031] Furthermore, the dispersion medium may be one or more of the following: soybean oil, rapeseed oil, wheat oil, methyl oleate, diesel oil, engine oil, and mineral oil.

[0032] Furthermore, the antifreeze is selected from one or more of ethylene glycol, propylene glycol, glycerol, or urea, or a mixture thereof;

[0033] Furthermore, the thickener is selected from one or more of xanthan gum, disintegrant, bentonite, carboxymethyl cellulose, or magnesium aluminum silicate;

[0034] Furthermore, the stabilizer is selected from one or more of the following: epoxidized soybean oil, epichlorohydrin, BHT, ethyl acetate, and triphenyl phosphate;

[0035] Furthermore, the disintegrant is selected from one or more of the following: bentonite, urea, aluminum chloride, low-substituted hydroxypropyl cellulose, lactose, citric acid, succinic acid, or sodium bicarbonate;

[0036] Furthermore, the defoamer is selected from silicone oil, silicone compounds, and C. 10 ~C 20 Saturated fatty acid compounds or C8-C 10 A mixture of one or more fatty alcohol compounds.

[0037] Furthermore, the pesticide composition can be formulated into pesticide-acceptable formulations, including solid formulations, liquid formulations, seed treatment agents, and other formulations;

[0038] Furthermore, the dosage form of the preparation is any one of emulsifiable concentrate, suspension concentrate, water-dispersible granules, water emulsion, microemulsion, granules, wettable powder, and dispersible oil suspension.

[0039] Furthermore, the formulation is any one of emulsifiable concentrate, suspension concentrate, water-dispersible granules, water emulsion, or dispersible oil suspension;

[0040] Application of a pesticide composition in the control of agricultural and forestry pests and sanitary pests;

[0041] Furthermore, the aforementioned agricultural and forestry pests and sanitary pests are Thysanoptera pests;

[0042] Furthermore, the aforementioned Thysanoptera pests are orchid thrips, western flower thrips, tobacco brown thrips, alfalfa flower thrips, oriental flower thrips, bellflower thrips, rice thrips, palm thrips, and tobacco thrips.

[0043] Furthermore, the aforementioned agricultural and forestry pests and sanitary pests are phytophagous mites;

[0044] Furthermore, the phytophagous mites are Tetranychus carmineus, Tetranychus truncatus, Tetranychus two-spotted, and Tetranychus citrus red spider mite;

[0045] Furthermore, the pesticide composition is applied in an effective dose to the pest that needs to be controlled or to the medium in which it grows.

[0046] The pesticide composition of the present invention has the following advantages:

[0047] 1) The composition of the present invention exhibits a synergistic effect within a certain ratio range, thereby improving the control effect of the composition on pests;

[0048] 2) The two active ingredients in the composition of the present invention have different mechanisms of action, which effectively delays the development of pesticide resistance in pests and extends the service life of the product;

[0049] 3) It is highly safe and suitable for all stages of crop growth, promoting crop growth and resulting in higher quality agricultural products. Detailed Implementation

[0050] The present invention will be further described below with reference to the embodiments. The percentages in the embodiments are all weight percentages, but the present invention is not limited thereto.

[0051] The compositions of the present invention can be provided in formulation form. They can be formulated as suspensions, water-dispersible granules, etc., as needed. The content of the active ingredient in the compositions of the present invention depends on the dosage when used alone, as well as on the mixing ratio and the degree of synergistic effect. The optimal range of active ingredient content varies depending on the type of formulation of the composition.

[0052] Example 1: 30% Formula (I)·flupyroxystrobin suspension (18:12)

[0053] Formula composition: 18% compound of formula (I), 12% flupyroxystrobin, 2% phenethylphenol polyether phosphate salt, 2% guerbert alcohol polyoxyethylene ether, 2% sodium alkyl polyoxyethylene ether sulfonate, 1.5% naphthalene sulfonate, 1% magnesium aluminum silicate, 0.15% xanthan gum, 0.01% carboxyethyl cellulose, 0.05% sodium sorbate, 5% ethylene glycol, 0.4% silicone defoamer, deionized water to make up the balance;

[0054] Preparation method: According to the formula ratio, the active ingredients, surfactants and other functional additives are placed in the reaction vessel in sequence, water is added and mixed evenly, and then subjected to high-speed shearing, wet sand milling and finally homogenization filtration to obtain the suspension product.

[0055] Example 2: 48% Formula (I)·flupyroxystrobin water-dispersible granules (24:24)

[0056] Formula composition: 24% of compound (I), 24% of flupyroxystrobin, 12% sodium lignosulfonate, 2.5% naphthalenesulfonate formaldehyde condensate, 2% sodium dodecyl sulfate, 4% sodium sulfate, and kaolin as a supplement;

[0057] Preparation method: According to the formula ratio, the active ingredients are added to the carrier, and surfactants and other functional additives are added to it. After mixing, the mixture is pulverized by air jet and 10-25% water is added. Then, the mixture is kneaded, granulated, dried and sieved to obtain the water-dispersible granule product.

[0058] Example 3: 16% Formula (I)·flupyroxystrobin dispersible oil suspension (7.5+8.5)

[0059] Formula composition: 7.5% compound of formula (I), 8.5% flupyroxystrobin, 1% naphthalene sulfonate condensate, 12% castor oil polyoxyethylene ether, 3% fatty alcohol polyoxyethylene ether, 1% calcium dodecylbenzene sulfonate, 1% sodium lignosulfonate, 0.5% silica, 0.5% organic bentonite, and soybean oil to make up the balance;

[0060] Preparation method: According to the formula ratio, the active ingredients, surfactants and other functional additives are placed in the reaction vessel in sequence, oil is added and mixed evenly, and then subjected to high-speed shearing, wet sand milling and finally homogenization filtration to obtain the dispersible oil suspension product.

[0061] Example 4: 25% Formula (I)·bentioflumin suspension (22:3)

[0062] Formula composition: 22% compound of formula (I), 3% bentioflumin, 2.5% sodium dodecyl sulfate, 1% naphthalene sulfonate formaldehyde condensate, 3% styrene phenol polyoxyethylene ether phosphate, 1.5% fatty alcohol polyoxyethylene ether, 1% carboxymethyl cellulose, 1% magnesium aluminum silicate, 0.2% xanthan gum, 5% propylene glycol, 0.02% potassium benzoate, 0.4% silicone defoamer, deionized water to make up the balance;

[0063] Preparation method: Same as in Example 1.

[0064] Example 5: 60% Formula (I)·bentioflumin water-dispersible granules (54:6)

[0065] Formula composition: 54% of compound (I), 6% bentioflumin, 8% naphthalene sulfonate formaldehyde condensate, 3% sodium lignosulfonate, 2% sodium polycarboxylate, 3% BX (a type of styrene), 2% ammonium sulfate, 0.5% urea, and kaolin (to make up the difference).

[0066] Preparation method: Same as in Example 3.

[0067] Example 6: 14% Formula (I)·bentioflumin emulsifiable concentrate (12+2)

[0068] Formulation composition: 12% compound of formula (I), 2% bentioflumin, 12% DMF, 10% styrene-phenol polyoxyethylene ether, 2% calcium dodecylbenzenesulfonate, 20% cyclohexanone, xylene to make up the balance;

[0069] Preparation method: Add the metered active ingredients, solvent, and co-solvent to a mixing tank and stir to dissolve them. Then add the emulsifier, and use the remaining solvent to make up the balance. Stir evenly in a mixing tank, and filter to obtain the emulsifiable oil product.

[0070] Example 7: 4% Formula (I)·bentioflumin water emulsion (3.6+0.4)

[0071] Formula composition: 3.6% compound of formula (I), 0.4% bentioflumin, 15% cyclohexanone, 5% EO / PO block polyether, 1% fatty alcohol polyoxyethylene ether phosphate, 5% ethylene glycol, 0.1% silicone defoamer, deionized water to make up the balance;

[0072] Preparation method: According to the formula ratio, the active ingredients are completely dissolved in the solvent, and then an emulsifier is added to form an oil phase; the dispersant, antifreeze, deionized water, etc. are stirred evenly to form an aqueous phase; the oil phase is added to the aqueous phase and stirred evenly, and the particles are sheared at high speed until the particle size is qualified. After adding the defoamer, the mixture is stirred evenly to obtain the water emulsion product.

[0073] Indoor bioactivity assay

[0074] Indoor Example 1: Co-toxicity of Compound (I) in combination with flupyroxystrobin on thrips

[0075] Test method: Drug film rolling tube method;

[0076] Test insect source: western flower thrips;

[0077] Test reagents: Compound of formula (Ⅰ) and flupyroxystrobin technical were provided by the Group's R&D Center;

[0078] Preparation of the drug: The original drug was prepared into a stock solution using an organic solvent (acetone), and then five series of mass concentrations were prepared using a 0.1% Tween 80 aqueous solution in equal proportions.

[0079] Experimental operation steps:

[0080] Immersion tubes: Glass tubes 7 cm high and 1.5 cm in diameter. The test pesticide was diluted with acetone to five concentrations. 250 μL of each concentration was added to the glass tube using a pipette. The tube was then held horizontally, and the palm of the hand was used to slowly roll the tube to accelerate the evaporation of the insecticide, ensuring even adhesion to the inner wall of the tube. Each tube constituted one replicate, with four replicates for each concentration. Acetone-immersed tubes served as a control.

[0081] Leaf dipping: Fresh cabbage leaves were punched into small round pieces with a diameter of 1 cm using a puncher. Each piece was dipped in a different concentration of the pesticide for 10 seconds, with a 0.1% Tween 80 solution used as a control. The treated leaves were then placed on filter paper to dry and then inserted into glass tubes containing the corresponding pesticide concentrations using tweezers.

[0082] Inoculation: Use a suction device to transfer 25 identical second-instar thrips nymphs into a glass tube, and seal the tube opening with a 200-mesh gauze.

[0083] The treated test insects were placed in a rearing room with a temperature of 25±0.1℃, a humidity of 60%~80%, and a light-dark ratio of 16h:8h for rearing and observation.

[0084] Investigation time: Investigate the mortality of test insects 36 hours after treatment, and record the total number of insects and the number of dead insects.

[0085] Criteria for determining death: If there is no reaction when the insect is lightly touched with the tip of a brush, it is considered dead.

[0086] Calculate the mortality rate:

[0087]

[0088] In the formula:

[0089] P – Mortality rate, expressed as a percentage (%);

[0090] K represents the number of dead insects, in heads;

[0091] N represents the total number of insects treated, in units of heads.

[0092]

[0093] In the formula:

[0094] P1 – Corrected mortality rate, in percentage (%);

[0095] Pt —The mortality rate is expressed as a percentage (%).

[0096] P0 – Mortality rate in the blank control group, expressed as a percentage (%).

[0097] If the control mortality rate is <5%, no correction is needed; if the control mortality rate is between 5% and 20%, correction should be performed according to the above formula; if the control mortality rate is >20%, the experiment needs to be repeated.

[0098] Linear regression analysis (DPS) between the probability value of mortality and the logarithm of a series of concentrations was used to determine the regression equation, r-value, and LC for each drug. 50 The values ​​and their 95% confidence intervals were used to calculate the co-toxicity coefficient (CTC) of the mixture using the Sun Yunpei method, which was used to evaluate the activity of the test agent on the test insects.

[0099] The specific calculation formula is as follows:

[0100]

[0101] In the formula:

[0102] ATI – Actual Measured Toxicity Index of Mixtures;

[0103] S – LC50 of standard insecticides 50 The unit is milligrams per liter (mg / L);

[0104] M – LC of the mixture 50 The unit is milligrams per liter (mg / L).

[0105] TTI = TI A *P A +TI B *P B

[0106] In the formula:

[0107] TTI – Theoretical Toxicity Index of Mixtures;

[0108] TI A —A. Toxicity index of drug A;

[0109] P A —Percentage content of drug A in the mixture, expressed as percentage (%);

[0110] TI B —Toxicity index of drug B;

[0111] P B —Percentage content of agent B in the mixture, expressed as percentage (%).

[0112]

[0113] In the formula:

[0114] CTC – Cotoxicity Coefficient;

[0115] ATI – Actual Measured Toxicity Index of Mixtures;

[0116] TTI – Theoretical Toxicity Index of Mixtures.

[0117] According to the criteria for classifying combined effects: a co-toxicity coefficient (CTC) ≥ 120 indicates an synergistic effect, a co-toxicity coefficient (CTC) ≤ 80 indicates an antagonistic effect, and 80 < CTC < 120 indicates an additive effect.

[0118] Experimental results:

[0119] Table 1 shows that the combination of compound (I) and flupyroxystrobin has a good control effect on second-instar nymphs of western flower thrips. The co-toxicity coefficient is greater than 130 when the weight ratio of compound (I) to flupyroxystrobin is 1:20–25:1, exhibiting a good synergistic effect; among them, the co-toxicity coefficient is the highest at a weight ratio of 3:2, which is 216.323.

[0120] Table 1 shows the results of indoor toxicity tests on compound (I) in combination with flupyroxystrobin against western flower thrips nymphs.

[0121]

[0122] Indoor Example 2

[0123] Synvirulence of compound (I) in combination with bentioflumin on Tetranychus cinnabarinus

[0124] Test reference: NY / T 1154.7-2006 "Indoor Biochemical Testing Guidelines for Pesticides - Part 7: Determination of Combined Effects of Mixtures".

[0125] Test insect source: Tetranychus carmineus (raised at the R&D center);

[0126] Test reagents: Compound of formula (Ⅰ) and bentioflumin technical were provided by the Group's R&D Center;

[0127] Test material preparation: Select mites (adult female mites) that are kept indoors and have the same physiological condition. Cut double-sided tape into 2cm lengths and stick them to one end of a glass slide. Then select healthy mites and stick their backs onto the double-sided tape. Place 30 mites per slide and put them in a container lined with a damp sponge. Cover the container and place it at (25±1)℃. After 2 hours, examine the slide under a microscope, remove any dead or injured individuals, and replenish the slide to 30 mites.

[0128] Reagent preparation: The original drug was prepared into a stock solution with acetone, and the stock solution was prepared into test reagents in different proportions with a 0.1% Tween-80 aqueous solution.

[0129] Drug treatment: Immerse the glass slide in the drug solution and gently shake for 5 seconds, then remove it, absorb the excess drug solution with absorbent paper, place it in a white porcelain dish with a damp sponge, cover it with a transparent plastic film, and keep it at (25±1)℃ for observation.

[0130] Each treatment was repeated four times, with a blank control group consisting of a treatment without the drug.

[0131] Experimental investigation: The mortality of test insects was checked 48 hours after treatment, and the total number of insects and the number of dead insects were recorded.

[0132] Calculate the mortality rate:

[0133]

[0134] In the formula:

[0135] P – Mortality rate, expressed as a percentage (%);

[0136] K represents the number of dead insects, in heads;

[0137] N represents the total number of insects treated, in units of heads.

[0138]

[0139] In the formula:

[0140] P1 – Corrected mortality rate, in percentage (%);

[0141] P t —The mortality rate is expressed as a percentage (%).

[0142] P0 – Mortality rate in the blank control group, expressed as a percentage (%).

[0143] If the control mortality rate is <5%, no correction is needed; if the control mortality rate is between 5% and 20%, correction should be performed according to the above formula; if the control mortality rate is >20%, the experiment needs to be repeated.

[0144] Linear regression analysis (DPS) between the probability value of mortality and the logarithm of a series of concentrations was used to determine the regression equation, r-value, and LC for each drug. 50 The values ​​and their 95% confidence intervals were used to calculate the co-toxicity coefficient (CTC) of the mixture using the Sun Yunpei method, which was used to evaluate the activity of the test agent on the test insects.

[0145] The specific calculation formula is as follows:

[0146]

[0147] In the formula:

[0148] ATI – Actual Measured Toxicity Index of Mixtures;

[0149] S – LC50 of standard acaricides 50 The unit is milligrams per liter (mg / L);

[0150] M – LC of the mixture 50 The unit is milligrams per liter (mg / L).

[0151] TTI = TI A *P A +TI B *P B

[0152] In the formula:

[0153] TTI – Theoretical Toxicity Index of Mixtures;

[0154] TI A —A. Toxicity index of drug A;

[0155] P A —Percentage content of drug A in the mixture, expressed as percentage (%);

[0156] TI B —Toxicity index of drug B;

[0157] P B —Percentage content of agent B in the mixture, expressed as percentage (%).

[0158]

[0159] In the formula:

[0160] CTC – Cotoxicity Coefficient;

[0161] ATI – Actual Measured Toxicity Index of Mixtures;

[0162] TTI – Theoretical Toxicity Index of Mixtures.

[0163] According to the criteria for classifying combined effects: a co-toxicity coefficient (CTC) ≥ 120 indicates an synergistic effect, a co-toxicity coefficient (CTC) ≤ 80 indicates an antagonistic effect, and 80 < CTC < 120 indicates an additive effect.

[0164] Experimental results:

[0165] The combined toxicity assays of compound (I) with bentioflumin at different ratios against *Tetranychus cinnabarinus* (adult female mites) are shown in Table 1. The results indicate that compound (I) exhibits LC50-like activity against *Tetranychus cinnabarinus*. 50 The value was 17.343 mg / L, and the LC50 value of bentioflumin against Tetranychus cinnabarinus was [missing value]. 50 The value was 0.366 mg / L.

[0166] Compound (I) did not show antagonistic effects against Tetranychus carmine when mixed with bentioflumin in a ratio of 1:27–35:1. However, compound (I) showed a synergistic effect with bentioflumin in a ratio of 1:20–23:1. The highest co-toxicity coefficient (197.293) was observed when the ratio of compound (I) to bentioflumin was 9:1. 50 The value was 1.559 mg / L.

[0167] Table 2 shows the results of indoor activity assays of compound (I) and bentioflumin in Tetranychus cinnabarinus (female adult mite).

[0168]

[0169] Field efficacy examples

[0170] Field control efficacy of compound (I) mixed with flupyroxystrobin against thrips

[0171] Experimental crops and targets: cucumber, thrips;

[0172] Experiment location: Cucumber greenhouse in Qingzhou City, Shandong Province;

[0173] Test date: July 22, 2022;

[0174] Experimental environment: The cultivation conditions in all experimental plots were uniform and the same level of fertilizer and water management was used;

[0175] Test reagents:

[0176] Table 3 Experimental Design

[0177] deal with test reagents <![CDATA[Active ingredient application rate g a i / hm 2 > 1 30% Formula (I)·flupyroxystrobin suspension (18:12) 20 2 5% Formula (I) compound emulsion 200 3 10% flupyroxystrobin suspension 30 4 Water comparison /

[0178] Experimental method: Apply the pesticide once during the peak period of thrips infestation.

[0179] Cell size and duplication: randomized block arrangement, 25m per cell 2 Each treatment was repeated 4 times.

[0180] Survey method: Five random sampling points were taken in each community. Ten cucumber leaves were collected at each point and tagged. Thrips on the cucumber leaves were knocked into a rice bucket lined with filter paper using a knocking method. The number was counted.

[0181] Before applying the pesticide, the initial insect population was investigated. One day and seven days after application, the number of thrips surviving on the leaves of each treatment was investigated, and the control effect was calculated.

[0182] Calculation formulas and data analysis:

[0183]

[0184] Results of field efficacy trials:

[0185] Experimental results showed that 30% (I) flupyroxystrobin suspension (18:12) had a good control effect on thrips, with control efficacy exceeding 93% at 1 day and 7 days after application, demonstrating significant control efficacy. The pesticide composition combining the aforementioned (I) compound and flupyroxystrobin also showed good control effect on thrips pests.

[0186] Table 4 shows the field efficacy of compound (I) in combination with flupyroxystrobin for controlling thrips.

[0187]

[0188] Note: The data in the table are the average of four replicates, with values ​​rounded to two decimal places. Letters indicate significance (0.05 / DMRT method).

[0189] Field Trial 2: Field Efficacy Trial for Controlling Red Spider Mites in Cotton

[0190] Test crop: cotton

[0191] Test subject: Cotton red spider mite

[0192] Test time and location: The test will be conducted in late May 2023 in Jining City, Shandong Province;

[0193] Experimental field conditions: The experimental site had moderate soil fertility, flat terrain, uniform fertility, and good irrigation conditions. All experimental plots had uniform cultivation conditions (soil type, fertilization, tillage, row spacing, etc.) and were consistent with local Good Agricultural Practices (GAP).

[0194] Experimental design: The formulation treatment, control treatment, and blank control were arranged in a randomized block design with a plot size of 20m². 2 Repeat 4 times.

[0195] Table 5. Treatments and dosages of different test reagents

[0196]

[0197] Application equipment: WS-16D Guardian electric sprayer, with a single fan-shaped mist nozzle, working pressure 0.15-0.4Mpa. Accurately measure the amount of pesticide according to the dosage requirements and the area of ​​the plot.

[0198] The weather was good during the experiment. On the day of application, the highest temperature was 32℃, the lowest temperature was 24℃, and the relative humidity was 72%.

[0199] Investigation methods: Before applying the pesticide, investigate the initial number of mites. For each plot, 50 leaves are labeled on each plant for investigation. The number of mites before treatment should not be less than 500. The number of live mites is investigated 3 days and 7 days after application.

[0200] Methods for calculating drug efficacy:

[0201]

[0202] During the experiment, cotton growth was observed to be good in all treatment plots, and no herbicide damage was observed in any treatment.

[0203] Results of experiments on the control of cotton spider mites with various pesticide treatments:

[0204] Table 6. Control efficacy of different tested pesticides against cotton red spider mite.

[0205]

[0206]

[0207] Note: The data in the table are the average of four replicates, with values ​​rounded to two decimal places. Letters indicate significance (0.05 / DMRT method).

[0208] As can be seen from the field test results of different tested agents against cotton red spider mites in Table 6, the pesticide composition obtained by compounding formula (I) with bentioflumin has good rapid and sustained effects against cotton red spider mites.

[0209] Three days after application, the efficacy of 14% Formula (I)·bentioflumin EC (12+2) at active ingredient dosages of 30 and 20 mg / kg was 84.80% and 80.28%, respectively. Seven days after application, the efficacy of 14% Formula (I)·bentioflumin EC (12+2) at active ingredient dosages of 30 and 20 mg / kg was 91.83% and 89.77%, respectively. Compared with the control single-dose formulation, there were significant differences at the 0.05 level, and the efficacy was higher than that of the single-dose formulation.

[0210] Although the present invention has been described in detail above with general description and specific embodiments, some modifications or improvements can be made to it, which will be obvious to those skilled in the art. Therefore, all such modifications or improvements made without departing from the spirit of the present invention are within the scope of protection claimed by the present invention.

Claims

1. A pesticide composition, characterized in that: The pesticide composition comprises active ingredient A and active ingredient B, wherein active ingredient A is a compound of formula (I) with the following chemical structural formula: (I) The active ingredient B is either flupyroxystrobin or bentioflumin.

2. The pesticide composition according to claim 1, characterized in that: The mass ratio of active ingredient A to active ingredient B is 1:27 to 35:

1.

3. The pesticide composition according to claim 1, characterized in that: The mass ratio of the compound of formula (I) to flupyroxystrobin is 1:20 to 25:1, and the mass ratio of the compound of formula (I) to bentioflumin is 1:20 to 23:

1.

4. The pesticide composition according to claim 1, characterized in that: Based on a total mass of 100 wt% of the pesticide composition, the sum of the contents of active ingredient A and active ingredient B in the pesticide composition is 0.5% to 90%.

5. The pesticide composition according to claim 1, characterized in that: The pesticide composition further includes adjuvants, which include carriers and adjuvants.

6. The pesticide composition according to claim 1, characterized in that: The pesticide composition can be formulated into pesticide-acceptable formulations, including solid formulations and liquid formulations.

7. The pesticide composition according to claim 6, characterized in that: The dosage form of the formulation is any one of emulsifiable concentrate, suspension concentrate, water-dispersible granules, water emulsion, microemulsion, granules, wettable powder, and dispersible oil suspension. The preferred formulation is any one of emulsifiable concentrate, suspension concentrate, water-dispersible granules, water emulsion, or dispersible oil suspension.

8. The application of the pesticide composition according to any one of claims 1-7 in the control of agricultural and forestry pests and sanitary pests.

9. The application according to claim 8, characterized in that: The pesticide composition described above targets thrips and Tetranychus cinnabarinus.

10. The application according to claim 8, characterized in that: The pesticide composition is applied in an effective dose to the pest that needs to be controlled or to the medium in which it grows.

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