Fermented feed for improving immunity and preventing and treating bluetongue of sheep and preparation method of fermented feed

Abstract

The invention belongs to the technical field of fermentation. The invention provides a fermented feed for improving immunity and preventing and treating bluetongue of sheep and a preparation method of the fermented feed. The preparation method comprises the following steps: (1) taking stalks of fresh commelina communis and Phaseolus calcaratus, squeezing the stalks of the fresh commelina communis and the stalks of the Phaseolus calcaratus respectively, removing residues, taking juice, and freeze-drying the obtained juice into powder which is named as freeze-dried commelina communis stalk juice powder and freeze-dried Phaseolus calcaratus stalk juice powder respectively; (2) mixing the freeze-dried powder of the commelina communis stem juice and the freeze-dried powder of the Phaseolus calcaratus stem juice, mixing the mixed freeze-dried powder and soybean meal to serve as a fermentation substrate, and inoculating bacillus subtilis for fermentation to obtain a fermented product; and (3) mixing the obtained fermented product, bran and rice bran, and then mixing with a premix to obtain the feed. The traditional Chinese medicine composition can improve the immunity of lambs, has a direct inactivation effect on bluetongue viruses, and remarkably reduces the fatality rate of the bluetongue viruses on the lambs.

Description

Technical Field

[0001] This invention belongs to the field of fermentation technology, specifically feed technology, and relates to a fermented feed that enhances immunity and prevents bluetongue disease in sheep, as well as its preparation method. Background Technology

[0002] Bluetongue disease is a serious infectious disease of ruminants caused by the bluetongue virus. It can infect sheep of all ages. Maternal animals can be infected through semen, and infected mothers can also transmit the virus vertically to their fetuses. Lambs, with their weak immune systems, are highly susceptible to infection and death.

[0003] Currently, the prevention and control of bluetongue disease mainly relies on conventional methods such as quarantine management, early culling of infected animals, and injection of hyperimmune serum. While these methods are relatively effective, they are time-consuming, labor-intensive, and have high labor costs. Furthermore, sheep farms in the mountainous areas of Southwest China are scattered, and there is a relative shortage of personnel, making it difficult to carry out related management work by relying primarily on these methods. Therefore, developing feed that can improve lamb immunity and significantly reduce the mortality rate of bluetongue disease would help reduce feeding and management costs and promote the development of the sheep farming industry. Summary of the Invention

[0004] In view of the shortcomings of the existing technology, the purpose of this invention is to provide a feed with readily available raw materials that can effectively prevent and treat bluetongue disease in lambs and improve their immunity, as well as a method for preparing the feed.

[0005] To achieve the above objectives, the present invention provides the following technical solution: A method for preparing fermented feed that enhances immunity and prevents bluetongue disease in sheep, the method comprising the following steps: (1) Take fresh dayflower and red adzuki bean stems, press them separately and discard the residue to extract the juice. Freeze-dry the obtained juice into powder and name them dayflower stem juice freeze-dried powder and red adzuki bean stem juice freeze-dried powder respectively. (2) Mix the freeze-dried powder of Dayflower stem juice and the freeze-dried powder of Weining red bean stem juice at a weight ratio of 3:1. Mix the freeze-dried powder and soybean meal at a weight ratio of 1:40~50 as a fermentation substrate, inoculate with Bacillus subtilis for fermentation, and obtain fermented product. (3) Mix 75-80 parts of the fermented product obtained in step (2), 5 parts of wheat bran, and 5-8 parts of rice bran according to the weight ratio, and then mix with the premix composed of trace elements and multivitamins to obtain feed.

[0006] Preferably, the weight ratio of the mixed freeze-dried powder to soybean meal is 1:40.

[0007] Preferably, during the fermentation process, the water content of the fermentation substrate is controlled to be 20% before fermentation.

[0008] Preferably, the Bacillus subtilis is preserved under the accession number CGMCC No. 5686 during fermentation.

[0009] Preferably, the inoculum amount of Bacillus subtilis during fermentation is 5%.

[0010] Preferably, the composition and addition amount of the premix composed of trace elements and multidimensional components in the resulting feed are as follows: ferrous sulfate 50mg / kg, copper sulfate 8mg / kg, zinc sulfate 100mg / kg, manganese sulfate 50mg / kg, cobalt chloride 1mg / kg, sodium selenite 0.5mg / kg, potassium iodide (calculated as iodine) 1mg / kg, vitamin A 12000IU / kg, vitamin D3 2000IU / kg, and vitamin E 3mg / kg.

[0011] Preferably, in step (3), 80 parts by weight of the fermented product obtained in step (2), 5 parts of wheat bran, and 8 parts of rice bran are mixed, and then mixed with a premix composed of trace elements and multivitamins to obtain feed.

[0012] Preferably, in step (1), fresh dayflower and red adzuki bean stems are taken, cut into small sections of 1-2 cm in length, pressed and discarded to extract juice. For the juice sticking to the wall, it is rinsed with 20°C water and mixed with the extracted juice.

[0013] A fermented feed for enhancing immunity and preventing bluetongue disease in sheep, said fermented feed being prepared by the aforementioned preparation method.

[0014] The aforementioned fermented feed was used in the preparation of a formulation for treating bluetongue virus infection in lambs.

[0015] The beneficial effects of this invention are: This invention can significantly increase the levels of IgA, IgM, IgG, and IL-6 in lamb serum, thereby enhancing lamb immunity. As the main component of the feed obtained by this invention, the fermented product has a certain direct inactivation effect on bluetongue virus. In challenge experiments, the feed of this invention can significantly reduce the mortality rate of lambs caused by bluetongue virus. Furthermore, the raw materials of this invention are readily available and inexpensive, all being common succulent weeds found in Southwest China. In summary, this invention has good industrial application value. Detailed Implementation

[0016] The present invention will be specifically described below through embodiments. It should be noted that the following embodiments are only used to further illustrate the present invention and should not be construed as limiting the scope of protection of the present invention. Some non-essential improvements and adjustments made by those skilled in the art based on the above-described invention are still within the scope of protection of the present invention.

[0017] Example 1

[0018] 1. Raw materials: Bacillus subtilis: accession number CGMCC No. 5686, donated to Guizhou Provincial Institute of Animal Husbandry and Veterinary Medicine, which purchased it from China General Microbiological Culture Collection Center; Dayflower and Weining red beans: donated to local farmers and collected from their contracted land; Soybean meal, wheat bran, and rice bran: purchased from the local market; Ferrous sulfate, copper sulfate, zinc sulfate, manganese sulfate, cobalt chloride, sodium selenite, potassium iodide, vitamin A, vitamin B1, vitamin C2: all are feed grade and are available in the laboratory.

[0019] 2. Feed preparation: (1) Take fresh dayflower stems and red adzuki bean stems, cut them into small sections (1-2cm), press them separately and discard the residue to extract the juice (for the juice sticking to the wall, rinse it with 20℃ water and mix it with the extracted juice); freeze-dry the obtained juice into powder, and name them dayflower stem juice freeze-dried powder and red adzuki bean stem juice freeze-dried powder respectively, for later use.

[0020] (2) Mix the freeze-dried powder of dayflower stem juice and the freeze-dried powder of red bean stem juice in Weining at a weight ratio of 3:1; mix the freeze-dried powder and soybean meal in a weight ratio of 1:40 as the fermentation substrate, add sterile water to control the water content of the fermentation substrate to 20%, and inoculate Bacillus subtilis at an inoculation rate of 5% to carry out fermentation to obtain the fermented product; (3) According to the weight proportions, 80 parts of the fermented product obtained in step (2), 5 parts of wheat bran, and 8 parts of rice bran are mixed and then mixed with the premix to obtain feed; in the obtained feed, the components and addition amounts of the premix are: ferrous sulfate 50mg / kg, copper sulfate 8mg / kg, zinc sulfate 100mg / kg, manganese sulfate 50mg / kg, cobalt chloride 1mg / kg, sodium selenite 0.5mg / kg, potassium iodide (calculated as iodine) 1mg / kg, vitamin A 12000IU / kg, vitamin D3 2000IU / kg, and vitamin E 3mg / kg.

[0021] Example 2

[0022] The raw materials are the same as in Example 1, and the feed preparation method is as follows: (1) Take fresh dayflower stems and red adzuki bean stems, cut them into small sections (1-2cm), press them separately and discard the residue to extract the juice (for the juice sticking to the wall, rinse it with 20℃ water and mix it with the extracted juice); freeze-dry the obtained juice into powder, and name them dayflower stem juice freeze-dried powder and red adzuki bean stem juice freeze-dried powder respectively, for later use.

[0023] (2) Mix the freeze-dried powder of Dayflower stem juice and the freeze-dried powder of Weining red bean stem juice at a weight ratio of 3:1; mix the freeze-dried powder and soybean meal at a weight ratio of 1:45 as the fermentation substrate, add sterile water to control the water content of the fermentation substrate to 20%, and inoculate Bacillus subtilis at an inoculation rate of 5% to carry out fermentation to obtain the fermented product; (3) According to the weight proportions, 75 parts of the fermented product obtained in step (2), 5 parts of wheat bran, and 8 parts of rice bran are mixed and then mixed with the premix to obtain feed; in the obtained feed, the components and addition amounts of the premix are: ferrous sulfate 50mg / kg, copper sulfate 8mg / kg, zinc sulfate 100mg / kg, manganese sulfate 50mg / kg, cobalt chloride 1mg / kg, sodium selenite 0.5mg / kg, potassium iodide (calculated as iodine) 1mg / kg, vitamin A 12000IU / kg, vitamin D3 2000IU / kg, and vitamin E 3mg / kg.

[0024] Example 3

[0025] The raw materials are the same as in Example 1, and the feed preparation method is as follows: (1) Take fresh dayflower stems and red adzuki bean stems, cut them into small sections (1-2cm), press them separately and discard the residue to extract the juice (for the juice sticking to the wall, rinse it with 20℃ water and mix it with the extracted juice); freeze-dry the obtained juice into powder, and name them dayflower stem juice freeze-dried powder and red adzuki bean stem juice freeze-dried powder respectively, for later use.

[0026] (2) Mix the freeze-dried powder of dayflower stem juice and the freeze-dried powder of red bean stem juice in Weining at a weight ratio of 3:1; mix the freeze-dried powder and soybean meal in a weight ratio of 1:50 as the fermentation substrate, add sterile water to control the water content of the fermentation substrate to 20%, and inoculate Bacillus subtilis at an inoculation rate of 5% to carry out fermentation to obtain the fermented product; (3) According to the weight proportions, 80 parts of the fermented product obtained in step (2), 5 parts of wheat bran, and 5 parts of rice bran are mixed together, and then mixed with the premix to obtain feed; in the obtained feed, the components and addition amounts of the premix are: ferrous sulfate 50mg / kg, copper sulfate 8mg / kg, zinc sulfate 100mg / kg, manganese sulfate 50mg / kg, cobalt chloride 1mg / kg, sodium selenite 0.5mg / kg, potassium iodide (calculated as iodine) 1mg / kg, vitamin A 12000IU / kg, vitamin D3 2000IU / kg, and vitamin E 3mg / kg.

[0027] Comparative Example 1 Compared to Example 1, the freeze-dried powder of dayflower stem juice was not used as a fermentation substrate when preparing the feed; otherwise, it was the same as Example 1.

[0028] The raw materials used in this comparative example 1 are the same as those used in example 1.

[0029] Feed preparation: (1) Take fresh Weining red bean stems, cut them into small sections (1-2cm), press them and discard the residue to extract the juice (for the juice sticking to the wall, rinse it with 20℃ water and mix it with the extracted juice); freeze-dry the obtained juice into powder and name it Weining red bean stem juice freeze-dried powder for later use.

[0030] (2) Mix the freeze-dried powder of Weining red bean stem juice and soybean meal at a weight ratio of 1:160 as the fermentation substrate, add sterile water to control the water content of the fermentation substrate to 20%, and inoculate Bacillus subtilis at an inoculation rate of 5% to obtain the fermented product. (3) According to the weight proportions, 80 parts of the fermented product obtained in step (2), 5 parts of wheat bran, and 8 parts of rice bran are mixed and then mixed with the premix to obtain feed; in the obtained feed, the components and addition amounts of the premix are: ferrous sulfate 50mg / kg, copper sulfate 8mg / kg, zinc sulfate 100mg / kg, manganese sulfate 50mg / kg, cobalt chloride 1mg / kg, sodium selenite 0.5mg / kg, potassium iodide (calculated as iodine) 1mg / kg, vitamin A 12000IU / kg, vitamin D3 2000IU / kg, and vitamin E 3mg / kg.

[0031] Comparative Example 2 Compared to Example 1, the freeze-dried powder of Weining red bean stem juice was not used as a fermentation substrate when preparing the feed; otherwise, it was the same as Example 1.

[0032] The raw materials used in this comparative example 2 are the same as those used in example 1.

[0033] Feed preparation: (1) Take fresh dayflower stems, cut them into small sections (1-2cm), press them and discard the residue to extract the juice (for the juice sticking to the wall, rinse it with 20℃ water and mix it with the extracted juice); freeze-dry the obtained juice into powder and name it dayflower stem juice freeze-dried powder for later use.

[0034] (2) Mix the freeze-dried powder of dayflower stem juice and soybean meal at a weight ratio of 3:160 as the fermentation substrate, add sterile water to control the water content of the fermentation substrate to 20%, and inoculate with Bacillus subtilis at an inoculation rate of 5% to obtain the fermented product. (3) According to the weight proportions, 80 parts of the fermented product obtained in step (2), 5 parts of wheat bran, and 8 parts of rice bran are mixed and then mixed with the premix to obtain feed; in the obtained feed, the components and addition amounts of the premix are: ferrous sulfate 50mg / kg, copper sulfate 8mg / kg, zinc sulfate 100mg / kg, manganese sulfate 50mg / kg, cobalt chloride 1mg / kg, sodium selenite 0.5mg / kg, potassium iodide (calculated as iodine) 1mg / kg, vitamin A 12000IU / kg, vitamin D3 2000IU / kg, and vitamin E 3mg / kg.

[0035] Comparative Example 3 Compared to Example 1, in preparing the feed, the freeze-dried powder of dayflower stem juice and the freeze-dried powder of red bean stem juice from Weining were not used as fermentation substrates; otherwise, they were the same as in Example 1.

[0036] The raw materials used in this comparative example 2 are the same as those used in example 1.

[0037] Feed preparation: (1) Using soybean meal as the fermentation substrate, add sterile water to control the water content of the fermentation substrate to 20%, and inoculate with Bacillus subtilis at an inoculation rate of 5% to carry out fermentation and obtain fermented product; (2) According to the weight proportions, 80 parts of the fermented product obtained in step (2), 5 parts of wheat bran, and 8 parts of rice bran are mixed and then mixed with the premix to obtain feed; in the obtained feed, the components and addition amounts of the premix are: ferrous sulfate 50mg / kg, copper sulfate 8mg / kg, zinc sulfate 100mg / kg, manganese sulfate 50mg / kg, cobalt chloride 1mg / kg, sodium selenite 0.5mg / kg, potassium iodide (calculated as iodine) 1mg / kg, vitamin A 12000IU / kg, vitamin D3 2000IU / kg, and vitamin E 3mg / kg.

[0038] Comparative Example 4 Compared to Example 1, this comparative example uses *Commelina communis* instead of *Commelina communis*. The *Commelina communis* used is also collected from local farmers' contracted land, and the rest is the same as in Example 1.

[0039] Feed preparation: (1) Take fresh stems of yellow quail and red adzuki bean from Weining, cut them into small sections (1-2cm), press them separately and discard the residue to extract the juice (for the juice sticking to the wall, rinse it with 20℃ water and mix it with the extracted juice); freeze-dry the obtained juice into powder, and name them freeze-dried powder of yellow quail stem juice and freeze-dried powder of red adzuki bean stem juice respectively, for later use.

[0040] (2) Mix the freeze-dried powder of the stem juice of *Gnaphalium affine* and the freeze-dried powder of the stem juice of *Vigna angularis* in Weining at a weight ratio of 3:1; mix the freeze-dried powder and soybean meal at a weight ratio of 1:40 as the fermentation substrate, add sterile water to control the water content of the fermentation substrate to 20%, and inoculate with Bacillus subtilis at an inoculation rate of 5% to ferment and obtain the fermented product. (3) According to the weight proportions, 80 parts of the fermented product obtained in step (2), 5 parts of wheat bran, and 8 parts of rice bran are mixed and then mixed with the premix to obtain feed; in the obtained feed, the components and addition amounts of the premix are: ferrous sulfate 50mg / kg, copper sulfate 8mg / kg, zinc sulfate 100mg / kg, manganese sulfate 50mg / kg, cobalt chloride 1mg / kg, sodium selenite 0.5mg / kg, potassium iodide (calculated as iodine) 1mg / kg, vitamin A 12000IU / kg, vitamin D3 2000IU / kg, and vitamin E 3mg / kg.

[0041] Experimental Example 1 240 lambs (3 months old) were prepared, divided into groups of 30 each, with half males and half females per group. All groups had free access to food and water. They were fed different feeds (from Examples 1, 2, 3, Comparative Examples 1, 2, 3, and 4) for 30 consecutive days. After feeding, jugular vein blood was collected from all lambs, and serum was collected to determine serum immune factors (IgA, IgM, IgG, and IL-6). The control group feed was purchased from Linqu Liyuan Shengnong Feed Co., Ltd., under the brand name "Liyuan Shengnong," with the following guaranteed values: crude protein ≥19%, crude fiber ≥8%, crude ash ≥8%, calcium 0.4-0.8%, total phosphorus ≥0.5%, sodium chloride 0.4-0.8%, lysine ≥0.6%, and moisture ≤14%. The results are shown in Table 1.

[0042] Table 1

[0043] Table 1 shows that the present invention can improve the immunity of lambs. Compared with the control group, the contents of IgA, IgM, IgG, and IL-6 in Examples 1-3 are significantly increased. Furthermore, when the feed preparation method is adjusted, especially after omitting the use of freeze-dried powders of Commelina communis stem juice and Weining red bean stem juice as fermentation raw materials, there is no significant difference in various indicators compared with the control group. In addition, combined with the results of Comparative Example 4, it can be seen that using freeze-dried powders of Commelina communis stem juice and Weining red bean stem juice as fermentation raw materials, and employing Bacillus subtilis for fermentation, the fermentation products have a synergistic effect on improving the immunity of lambs.

[0044] Experimental Example 2 Prepare 240 lambs (3 months old), divided into groups of 30 each, with half males and half females per group. All groups had free access to food and water. Each group was fed a different feed derived from Examples 1, 2, 3, Comparative Examples 1, 2, 3, and 4, respectively. On the 7th day of feeding, each lamb in each group was injected with 1 mL of bluetongue virus culture solution (1×10⁻⁶). 5 (TCID50 / mL), and then continued feeding for 23 days. The sheep's body temperature was measured daily, and clinical symptoms were observed and mortality was recorded. The experimental results are shown in Table 2.

[0045] Table 2

[0046] As shown in Table 2, feeding lambs with the feed of this invention can have an effect of preventing death from bluetongue virus. Based on Table 1, it can be inferred that this effect is partly due to the enhanced immunity of the lambs.

[0047] Experimental Example 3 Antiviral (bluetongue virus) experiments were conducted on the fermentation products.

[0048] The fermentation products obtained in Examples 1 to 3 and Comparative Examples 1 to 4 were dried, pulverized, and sieved. The drying process was as follows: the fermentation products were drained of water, freeze-dried, then crushed, dispersed, and ground, and passed through a 200-mesh sieve to obtain fine powder.

[0049] The aforementioned fine powders were added to PBS solution to prepare a 1 mg / mL solution. The solution was then magnetically stirred at 25°C (2000 rpm) for 10 minutes, and all powders were found to dissolve completely. After complete dissolution, the solution was sterilized by passing it through a 0.22 μm filter membrane to obtain the stock solution.

[0050] The following protocol was used to conduct the antiviral experiment: Cell line selection: Vero (African green monkey kidney cells); Cell culture conditions: DMEM medium (high glucose) was used, with 10% fetal bovine serum and 1% penicillin-streptomycin solution added; cells were cultured in a constant temperature cell culture incubator at 37℃ and 5% CO2.

[0051] Passaging and seeding protocol: When the cell confluence reaches 80%-90%, the cells are digested and passaged using 0.25% trypsin. Before the experiment, healthy Vero cells are seeded at an appropriate density in 96-well cell culture plates and allowed to adhere and grow overnight to form a dense monolayer.

[0052] TCID 50 Determination: The original virus solution was serially diluted 10-fold, and each dilution was inoculated into multiple Vero cell wells. After 7 days of culture, the significant pathogenic effect (CPE) was observed. The Reed-Muench method was used to calculate the virus dilution that caused 50% of cells to develop pathogenicity, i.e., TCID. 50 .

[0053] TCID 100 50 The aforementioned stock solutions were added to the virus solution to prepare virus solutions with powder concentrations of 0.01 mg / mL and 0.1 mg / mL, respectively. These solutions were incubated at 37°C and 5% CO2 for 1 hour to infect cells. A virus control group was prepared by adding virus solution but not the stock solutions, while a cell control group was prepared by adding neither virus solution nor the stock solutions. Cytopathic effects were observed using an inverted optical microscope. Cell viability was recorded (using MTT staining) when cytopathic effects reached 90% in the virus control group and the cells in the cell control group showed normal activity. The experimental results are shown in Table 3.

[0054] Table 3

[0055] As shown in Table 3, the fermented product in the feed obtained by this invention has a certain inactivation effect on bluetongue virus and can improve the survival rate of the tested cells to a certain extent. Combining the results of Tables 1, 2 and 3, the inventors speculate that the feed of this invention can significantly reduce lamb mortality, not only because the fermented product has a certain virus inactivation effect, but also because the feed can improve the lamb's immunity.

Claims

1. A method for preparing fermented feed that enhances immunity and prevents bluetongue disease in sheep, characterized in that, The preparation method includes the following steps: (1) Take fresh dayflower and red adzuki bean stems, press them separately and discard the residue to extract the juice. Freeze-dry the obtained juice into powder and name them dayflower stem juice freeze-dried powder and red adzuki bean stem juice freeze-dried powder respectively. (2) Mix the freeze-dried powder of Dayflower stem juice and the freeze-dried powder of Weining red bean stem juice at a weight ratio of 3:

1. Mix the freeze-dried powder and soybean meal at a weight ratio of 1:40~50 as a fermentation substrate, inoculate with Bacillus subtilis for fermentation, and obtain fermented product. (3) Mix 75-80 parts of the fermented product obtained in step (2), 5 parts of wheat bran, and 5-8 parts of rice bran according to the weight ratio, and then mix with the premix composed of trace elements and multivitamins to obtain feed.

2. The method for preparing a fermented feed to enhance immunity and prevent bluetongue disease in sheep according to claim 1, characterized in that, The weight ratio of the mixed freeze-dried powder to soybean meal is 1:

40.

3. A method for preparing a fermented feed for enhancing immunity and preventing bluetongue disease in sheep according to claim 1 or 2, characterized in that, During the fermentation process, the water content of the fermentation substrate is controlled to be 20% before fermentation.

4. The method for preparing a fermented feed to enhance immunity and prevent bluetongue disease in sheep according to claim 3, characterized in that, During fermentation, the Bacillus subtilis was preserved under the accession number CGMCC No. 5686.

5. The method for preparing a fermented feed to enhance immunity and prevent bluetongue disease in sheep according to claim 4, characterized in that, During fermentation, the inoculum size of Bacillus subtilis is 5%.

6. A method for preparing a fermented feed for enhancing immunity and preventing bluetongue disease in sheep according to claim 1 or 5, characterized in that, The composition and addition amount of the premixed feed composed of trace elements and multidimensional components in the resulting feed are as follows: ferrous sulfate 50mg / kg, copper sulfate 8mg / kg, zinc sulfate 100mg / kg, manganese sulfate 50mg / kg, cobalt chloride 1mg / kg, sodium selenite 0.5mg / kg, potassium iodide (calculated as iodine) 1mg / kg, vitamin A 12000IU / kg, vitamin D3 2000IU / kg, and vitamin E 3mg / kg.

7. The method for preparing a fermented feed to enhance immunity and prevent bluetongue disease in sheep according to claim 6, characterized in that, In step (3), 80 parts of the fermented product obtained in step (2), 5 parts of wheat bran, and 8 parts of rice bran are mixed according to the weight ratio, and then mixed with a premix composed of trace elements and multivitamins to obtain feed.

8. The method for preparing a fermented feed to enhance immunity and prevent bluetongue disease in sheep according to claim 7, characterized in that, In step (1), fresh dayflower and red adzuki bean stems are taken, cut into 1-2 cm long sections, pressed and discarded to extract the juice. The juice that sticks to the wall is rinsed with 20°C water and mixed with the extracted juice.

9. A fermented feed for enhancing immunity and preventing bluetongue disease in sheep, characterized in that, The fermented feed is prepared by the preparation method according to any one of claims 1 to 8.

10. The use of the fermented feed as described in claim 9 in the preparation of an agent for treating bluetongue virus infection in lambs.

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