Feed additive for improving animal production performance and preparation method thereof

Abstract

The invention provides a feed additive for improving animal production performance and a preparation method of the feed additive. The feed additive comprises the following components: 20-40 parts of astragalus membranaceus, 10-30 parts of angelica sinensis, 10-30 parts of tetrapanax papyriferus, 10-30 parts of liquidambar formosana hance, 10-20 parts of saccharomyces cerevisiae and 10-20 parts of bacillus subtilis. Compared with other similar products, the preparation prepared by the method has the advantages that the effect is obviously improved, and the cost is lower.

Description

Technical Field

[0001] This invention belongs to the field of feed in the biomedical field, and relates to a method for preparing a feed additive for improving animal production performance. Background Technology

[0002] With the continuous growth of the global population and the improvement of people's living standards, the demand for animal-based food is increasing. Protecting animal safety is very important. However, many livestock farmers now choose to add antibiotics to improve animal production performance and thus increase their profits.

[0003] However, long-term use of antibiotics can lead to many side effects. For example, long-term or excessive use of antibiotics can cause bacteria to develop drug resistance, rendering antibiotics ineffective and posing a threat to animal health. Antibiotics can also damage beneficial bacteria in the gut, leading to gut microbiota imbalance and affecting digestive function and the immune system. Antibiotics may remain in the animal's body and be passed to humans through the food chain, posing a potential risk to human health. The use of antibiotics may suppress the animal's immune system, increasing the risk of contracting other diseases.

[0004] To meet this demand, improving animal production performance has become a key task for the current livestock industry.

[0005] The Bacillus subtilis in this invention grows rapidly, has low nutritional requirements, can efficiently secrete many proteins and metabolites, and does not produce toxins, making it a safe, non-pathogenic microorganism.

[0006] Brewing yeast, a feed additive, has a beneficial effect on the digestive system of livestock and poultry, helping to improve the balance of intestinal microbiota, increase microbial activity, and enhance immunity. It also promotes the absorption of nutrients and improves feed utilization. Furthermore, brewing yeast is rich in protein, vitamins, and trace elements, which promote the growth and development of the livestock and poultry's immune system. As a highly efficient feed additive, brewing yeast can improve the production performance of livestock and poultry, increasing meat quality and protein content, accelerating weight gain, and improving the profitability of poultry farming.

[0007] The unique feature of this invention lies in the addition of traditional Chinese medicinal herbs, thereby improving animal production performance while reducing harm to the animals themselves. The characteristics of each herb in the formula are as follows: Astragalus is the dried root of a plant in the genus Astragalus of the legume family. It is sweet and warm in nature, and enters the lung and spleen meridians. It tonifies qi and raises yang, consolidates the exterior and stops sweating, promotes diuresis and reduces swelling, promotes pus drainage and detoxification, and astringes sores and promotes tissue regeneration. Its main indications are: deficiency of lung and spleen qi, sinking of middle qi, spontaneous sweating due to exterior deficiency, edema due to qi deficiency, and carbuncles that are difficult to heal and have been ulcerated for a long time. Astragalus can enhance the immune function of animals and improve their resistance to disease. At the same time, astragalus has a certain promoting effect on the growth and development of animals. Angelica is the dried root of a plant in the genus Angelica of the family Apiaceae. It is sweet and pungent in taste, and warm in nature. It enters the liver, heart, and spleen meridians. It nourishes blood, invigorates blood circulation, relieves pain, moistens dryness, and promotes bowel movements; its main indications are: blood deficiency and fatigue, blood stasis and pain, traumatic injuries, carbuncles and sores, constipation due to intestinal dryness, and various postpartum diseases; Angelica sinensis can improve blood circulation in animals, increase blood oxygen supply, and has a certain regulatory effect on the animal reproductive system, while also helping to enhance the animal's immune function; Tetrapanax papyriferus is the stem pith of a plant in the Araliaceae family, Tetrapanax papyriferus genus, with a sweet and bland taste and slightly cold nature. It enters the lung and stomach meridians. It clears heat and promotes diuresis, regulates qi and promotes lactation; its main indications are: damp-heat urinary tract infection, scanty and dark urine, edema, and insufficient lactation. Tetrapanax papyriferus can promote milk secretion in animals. Liquidambar formosana, also known as Liquidambar formosana fruit, is the dried mature fruit cluster of Liquidambar formosana, a plant in the Hamamelidaceae family, with a bitter and neutral taste. It enters the liver and kidney meridians. It dispels wind and invigorates collaterals, promotes diuresis and regulates menstruation; its main indications are: joint pain, limb contractures, insufficient lactation, and edema.

[0008] Astragalus and angelica are common natural plants that are both food and medicine. They are widely used in the pharmaceutical, health product, and animal feed industries.

[0009] Therefore, we are considering combining Astragalus membranaceus, Angelica sinensis, Tetrapanax papyriferus, Liquidambar formosana, Bacillus subtilis, and Saccharomyces cerevisiae to improve animal production performance and increase the economic benefits for farmers. Summary of the Invention

[0010] The present invention adopts the following technical solution:

[0011] This invention provides a feed additive for animal production performance, comprising the following components by weight: 20-40 parts Astragalus membranaceus, 10-30 parts Angelica sinensis, 10-30 parts Tetrapanax papyriferus, 10-30 parts Liquidambar formosana, 10-20 parts Bacillus subtilis, and 10-20 parts Saccharomyces cerevisiae.

[0012] The present invention has the following advantages:

[0013] (1) Products prepared using the method of the present invention can better improve the production performance of animals.

[0014] (2) The products prepared by the method of the present invention generate nearly zero solid waste.

[0015] A feed additive for improving animal production performance, comprising the following steps:

[0016] Step 1: Fermentation culture of Bacillus subtilis and Saccharomyces cerevisiae in liquid culture medium at a temperature of 32-37 degrees Celsius for 24-72 hours. The bioactivity was measured to be ≥1×10⁻⁶ per gram. 10 CFU culture can be stopped immediately.

[0017] Step 2: Spray dry the above culture medium. Set the parameters of the spray tower to an outlet temperature of 60 degrees and an inlet temperature of 120 degrees. The prepared material is ready for use.

[0018] Step 3: Take 20-40 parts of Astragalus membranaceus, 10-30 parts of Angelica sinensis, 10-30 parts of Tetrapanax papyriferus, and 10-30 parts of Liquidambar formosana. Soak them in 10 times the amount of water and extract them by reflux at 60 degrees Celsius for two hours. Filter the extract. Add 8 times the amount of water to the obtained Chinese herbal residue and extract it by reflux at 60 degrees Celsius for two hours. Filter the extract. Combine the two filtrates and concentrate them at 60 degrees Celsius. Spray dry the filtrate and use the prepared material for later use.

[0019] Step 4: Dry the extracted residue, crush it, and pass it through a 40-mesh sieve for later use.

[0020] Step 5: Take 20 portions of the mixture obtained in Step 1 and the evenly mixed aluminum foil bags obtained in Steps 2, 3 and 4, and package them separately. Detailed Implementation

[0021] Example 1

[0022] Step 1: Bacillus subtilis and Saccharomyces cerevisiae were fermented in liquid culture medium at a temperature of 32-37 degrees Celsius for 24-72 hours. The bioactivity was measured to be 1.1 × 10⁻⁶. 10 cfu / g, filter, and keep the filtrate for later use.

[0023] Step 2: Spray dry the above culture medium. Set the parameters of the spray tower to an outlet temperature of 60 degrees and an inlet temperature of 120 degrees. The prepared material is ready for use.

[0024] Step 3: Take 20 parts of Astragalus membranaceus, 20 parts of Angelica sinensis, 25 parts of Tetrapanax papyriferus, and 10 parts of Liquidambar formosana. Add 10 times the amount of water and soak. Reflux at 60 degrees Celsius for two hours. Filter the extract. Add 8 times the amount of water to the obtained Chinese herbal residue and reflux at 60 degrees Celsius for two hours. Filter the extract. Combine the two filtrates and concentrate the filtrate at 60 degrees Celsius. Spray dry the filtrate and use the prepared material for later use.

[0025] Step 4: Dry the extracted residue, crush it, and pass it through a 40-mesh sieve for later use.

[0026] Step 5: Take 20 portions of the mixture obtained in Step 1 and the evenly mixed aluminum foil bags obtained in Steps 2, 3 and 4, and package them separately.

[0027] Example 2

[0028] Step 1: Bacillus subtilis and Saccharomyces cerevisiae were fermented in liquid culture medium at a temperature of 32-37 degrees Celsius for 24-72 hours. The bioactivity was measured to be 1.07 × 10⁻⁶. 10 cfu / g, filter, and keep the filtrate for later use.

[0029] Step 2: Spray dry the above culture medium. Set the parameters of the spray tower to an outlet temperature of 60 degrees and an inlet temperature of 120 degrees. The prepared material is ready for use.

[0030] Step 3: Take 10 parts of Astragalus membranaceus, 20 parts of Angelica sinensis, 10 parts of Tetrapanax papyriferus, and 10 parts of Liquidambar formosana. Soak them in 10 times the amount of water and reflux at 60 degrees Celsius for two hours. Filter the extract. Soak the resulting Chinese herbal residue in 8 times the amount of water and reflux at 60 degrees Celsius for two hours. Filter the extract. Combine the two filtrates and concentrate them at 60 degrees Celsius. Spray dry the filtrate and use the prepared material for later use.

[0031] Step 4: Dry the extracted residue, crush it, and pass it through a 40-mesh sieve for later use.

[0032] Step 5: Take 20 portions of the mixture obtained in Step 1 and the evenly mixed aluminum foil bags obtained in Steps 2, 3 and 4, and package them separately.

[0033] Example 3

[0034] Take 40 parts of Astragalus membranaceus, 30 parts of Angelica sinensis, 30 parts of Tetrapanax papyriferus, and 30 parts of Liquidambar formosana. Soak them in 10 times the amount of water and reflux at 60 degrees Celsius for two hours. Filter the extract. Soak the resulting Chinese herbal residue in 8 times the amount of water and reflux at 60 degrees Celsius for two hours. Filter the extract. Combine the two filtrates and concentrate them at 60 degrees Celsius. Spray dry the filtrate. The prepared material can be packaged in aluminum foil bags.

[0035] Example 4

[0036] Step 1: Bacillus subtilis and Saccharomyces cerevisiae were fermented in liquid culture medium at a temperature of 32-37 degrees Celsius for 24-72 hours. The bioactivity was measured to be 1.1 × 10⁻⁶. 10 cfu / g, filter, and keep the filtrate for later use.

[0037] Step 2: Spray dry the above culture medium. Set the parameters of the spray tower to an outlet temperature of 60 degrees and an inlet temperature of 120 degrees. The resulting powder is then packaged into aluminum foil bags.

[0038] Example 5

[0039] Step 1: Bacillus subtilis and Saccharomyces cerevisiae were fermented in liquid culture medium at a temperature of 32-37 degrees Celsius for 24-72 hours. The bioactivity was measured to be 1.08 × 10⁻⁶. 10 cfu / g, filter, and keep the filtrate for later use.

[0040] Step 2: Spray dry the above culture medium. Set the parameters of the spray tower to an outlet temperature of 60 degrees and an inlet temperature of 120 degrees. The prepared material is ready for use.

[0041] Step 3: Take 40 parts of Astragalus membranaceus, 30 parts of Angelica sinensis, 30 parts of Tetrapanax papyriferus, and 30 parts of Liquidambar formosana. Soak them in 10 times the amount of water and reflux at 60 degrees Celsius for two hours. Filter the extract. Soak the resulting Chinese herbal residue in 8 times the amount of water and reflux at 60 degrees Celsius for two hours. Filter the extract. Combine the two filtrates and concentrate them at 60 degrees Celsius. Spray dry the filtrate and use the prepared material for later use.

[0042] Step 4: Dry the extracted residue, crush it, and pass it through a 40-mesh sieve for later use.

[0043] Step 5: Take 20 portions of the mixture obtained in Step 1 and the evenly mixed aluminum foil bags obtained in Steps 2, 3 and 4, and package them separately.

[0044] The following experiments will verify the effectiveness.

[0045] Experiment 1: Comparison of Milk Yields in Dairy Cows

[0046] Grouping: 60 healthy Holstein cows were selected from the dairy farm in Luoyang City and divided into 6 groups of 10 each.

[0047] Control group: A commercially available feed additive used to improve animal production performance.

[0048] Experimental group: Examples 1-5 of the present invention.

[0049] For the first 10 days of the experiment, the cows were fed according to the farmers' usual feeding habits, and the average daily milk production of each group was recorded as the baseline data. Starting from the 10th day, the control group was fed a commercially available feed additive for improving animal production performance, while the experimental group was fed the sample obtained in Examples 1-5, 200g each time. The milk production of the cows was recorded and compared after 10-15 days, 25-30 days, 40-45 days, and 55-60 days of feeding.

[0050] Table 1

[0051]

[0052]

[0053] The comparison in Table 1 proves that:

[0054] Example 1: Long-term feeding increased milk production by 0.69 kg / day compared to the control group.

[0055] Example 2: Long-term feeding increased milk production by 0.57 / day compared to the control group.

[0056] Example 3: Long-term feeding increased milk production by 0.51 / day compared to the control group.

[0057] Example 4: Long-term feeding increased milk production by 0.39g / day compared to the control group.

[0058] Example 5: Milk production increased significantly after 15 days of feeding, and steadily increased by 1.05 kg / head / day after 30 days of feeding. After long-term feeding, milk production increased by 1.69 kg / head / day compared with the control group.

[0059] The results showed that the feed additive prepared according to Example 5 was significantly more effective in increasing milk production in dairy cows than the control group and feed additives prepared in other proportions.

[0060] Experiment 2: Comparative Experiment on Piglet Weight Gain

[0061] Grouping: Sixty pregnant sows were selected from pig farms in Luoyang City and divided into 6 groups of 10 sows each.

[0062] Control group: A commercially available feed additive used to improve animal production performance.

[0063] Experimental group: Examples 1-5 of the present invention.

[0064] Piglets were weaned at 28 days old. Average weight gain and mortality rates of piglets were recorded and analyzed.

[0065] Table 2

[0066]

[0067]

[0068] Table 2 shows that the number of piglets that died when fed a commercially available feed additive for improving animal production performance was 5, while the number of piglets that died when fed the sample obtained in Example 5 was 1. The results indicate that the sample obtained in Example 5 has a better ability to improve animal production performance than the control group and the samples obtained in other proportions.

[0069] Conclusion: As shown in the table above, the feed additive prepared using Example 5 of the present invention can significantly improve the production performance of animals and improve the survival rate of animal offspring.

Claims

1. A feed additive for improving animal production performance, characterized in that, It consists of the following units of weight: Astragalus membranaceus 20-40 parts, Angelica sinensis 10-30 parts, Tetrapanax papyriferus 10-30 parts, Liquidambar formosana 10-30 parts, Bacillus subtilis powder 10-20 parts, Saccharomyces cerevisiae powder 10-20 parts.

2. A method for preparing a feed additive for animal production performance as described in claim 1, characterized in that, Includes the following steps: Step 1: Cultivate Bacillus subtilis and Saccharomyces cerevisiae; Step 2: The above culture medium is spray-dried into powder; Step 3: Take 20-40 parts of Astragalus membranaceus, 10-30 parts of Angelica sinensis, 10-30 parts of Tetrapanax papyriferus, and 10-30 parts of Liquidambar formosana for extraction. Concentrate the extract and dry the concentrate to obtain the extract. Step 4: Grind and mix.

3. The preparation method according to claim 2, characterized in that, The ratio of Bacillus subtilis to Saccharomyces cerevisiae is 1:

1.

4. The preparation method according to claim 2, characterized in that, The culture temperature of Bacillus subtilis and Saccharomyces cerevisiae is 32-37 degrees Celsius, and the culture time is 24-72 hours.

5. The preparation method according to claim 2, characterized in that, The bioactivity of Bacillus subtilis and Saccharomyces cerevisiae after culture was ≥1×10⁻⁶ per g. 10cfu .

6. The preparation method according to claim 2, characterized in that, The extraction method for the traditional Chinese medicine is reflux extraction at 60 degrees Celsius, and the drying method is spray drying.

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