Bearing chamber for cell co-incubation and application thereof

Abstract

The invention relates to the technical field of cell culture inserts, and particularly discloses a small bearing chamber for cell co-incubation and application of the small bearing chamber. The small bearing chamber comprises a cylindrical main body, a flange part and a percolation bottom plate; the flange part is fixedly assembled on the outer wall of the first end of the cylindrical main body, and the percolation bottom plate is fixedly assembled at a port of the second end of the cylindrical main body; when the small bearing chamber is used, the cylindrical main body is embedded in the hole of the pore plate, and the flange part is in lap joint with the edge of the hole to form axial limiting, so that the cylindrical main body and the percolation bottom plate are jointly suspended in the hole, and based on the structural design, the small bearing chamber can support a three-dimensional culture mode of cells. The small bearing chamber provided by the invention can be prepared by adopting a 3D printing process or a numerical control engraving process, the process is simple and convenient, parameters can be flexibly adjusted according to requirements so as to adapt to diversified experiment scenes, and the technical bottlenecks that a traditional cell culture plug-in is narrow in application scene, complex in preparation process and only capable of adopting a two-dimensional culture mode are solved.

Description

[0001] This application claims priority to Chinese Patent Application No. 202610073093.3, filed on January 20, 2026, entitled "A carrier chamber for cell co-incubation and its application thereto", the entire contents of which are incorporated herein by reference. Technical Field

[0002] This application relates to the field of cell culture plug-in technology, and in particular to a carrier chamber for cell co-incubation and its application. Background Technology

[0003] Cell culture inserts are core tools for in vitro cell experiments, and a commercial product system represented by CorningTranswell and Millicell has been formed in the market. The manufacturing technology of these traditional cell culture inserts is relatively mature. They are usually made of polymer materials such as polycarbonate, polyethylene terephthalate, or polytetrafluoroethylene, and products with fixed membrane pore size and fixed geometric dimensions are prepared through standardized processes. They are widely used in basic cell biology research.

[0004] However, as cell experiment technology develops towards precision, personalization, and three-dimensionality, traditional cell culture plug-in technology has gradually revealed several limitations, making it difficult to meet current experimental needs. Specific problems include: First, traditional cell culture plug-ins have fixed size specifications, primarily suitable for verifying cell migration behavior, and cannot be customized to adjust parameters according to experimental objectives. Second, these plug-ins are mostly single-use, and the cost per unit is high, significantly increasing overall usage costs. Third, the materials used in these plug-ins are concentrated in a few polymers such as polycarbonate, polyethylene terephthalate, and polytetrafluoroethylene, making it difficult to meet special experimental conditions. Fourth, the manufacturing process of traditional plug-ins is complex, requiring high-precision molds and specialized polymer processing equipment for production. Furthermore, existing technologies in the field of biocompatibility testing suffer from insufficient standardization, particularly lacking dedicated experimental platforms for novel three-dimensional biomaterials such as hydrogels and electrospun scaffolds. This makes it impossible to achieve stable fixation of such soft materials, leading to material leakage. At the same time, traditional plug-in systems are essentially based on two-dimensional culture modes, making it difficult to reproduce the three-dimensional growth microenvironment and intercellular interactions of cells in vivo. Consequently, the evaluation results of the biocompatibility and cell function regulation of novel biomaterials deviate significantly from the actual physiological state in vivo, making it difficult to accurately support subsequent research and application transformation.

[0005] Traditional cell culture plug-in technology is insufficient to meet current experimental needs, and there is an urgent need for a cell culture plug-in that is widely applicable, has a simple preparation process, and can realize three-dimensional culture mode. Summary of the Invention

[0006] In view of this, the purpose of this application is to provide a carrier chamber for cell co-incubation and its application, in order to solve the technical bottlenecks of traditional cell culture plug-ins, such as narrow applicable scenarios, complex preparation process, and the inability to realize only two-dimensional culture mode.

[0007] To achieve the above-mentioned technical objectives, this application provides a carrier chamber for cell co-incubation, comprising a cylindrical body, a flange, and a percolation bottom plate;

[0008] The outer wall of the first end of the cylindrical body is provided with a flange; the second end of the cylindrical body is provided with a percolation bottom plate. The cylindrical body and the percolation bottom plate enclose an internal chamber, which is used to hold the material.

[0009] The flange is used to overlap the edge of the perforation plate to suspend the internal chamber; the percolation bottom plate allows for material exchange between the internal chamber and the external space.

[0010] Furthermore, the cylindrical body, flange, and percolation base plate are integrally formed; the flange is formed by extending along the outer wall of the first end of the cylindrical body in a direction away from the axis; the percolation base plate is formed by extending along the second end port of the cylindrical body in a direction towards the axis.

[0011] Furthermore, the percolation substrate is provided with through holes, the diameter of which is 0.5~2.0mm; the porosity of the percolation substrate is 15~40%.

[0012] Furthermore, the cylindrical body is provided with through holes, the diameter of which is 0.5~2.0mm; the opening ratio of the cylindrical body is 15~40%.

[0013] Furthermore, the through holes are arranged in a matrix or honeycomb pattern.

[0014] Furthermore, the material supporting the chamber is one of PTFE, PAEK, PLA, TPU, or PEEK.

[0015] This application provides a method for preparing a carrier chamber for cell co-incubation, wherein the carrier chamber is prepared by 3D printing or CNC engraving.

[0016] This application provides a cell culture device, including a well plate and a support chamber for cell co-incubation;

[0017] One or more holes are provided on the perforated plate; the outer wall diameter of the cylindrical body supporting the chamber is smaller than the inner wall diameter of the hole; the maximum radial dimension of the flange portion supporting the chamber is larger than the inner wall diameter of the hole.

[0018] When the perforated plate is used in conjunction with the support chamber, the cylindrical body of the support chamber is embedded in the hole, and a gap is formed between the outer wall of the cylindrical body and the inner wall of the hole; the flange of the support chamber overlaps the edge of the hole, so that the internal cavity of the support chamber is suspended in the air, and the percolation bottom plate of the support chamber allows for material exchange between the internal cavity and the external space.

[0019] Furthermore, the dimensional parameters of the holes on the perforated plate are as follows: the inner wall diameter of the hole is 3~40mm, the side wall thickness of the hole is 0.5~1mm, and the depth of the hole is 3~12mm; the dimensional parameters of the supporting chamber are as follows: the outer wall diameter of the cylindrical body is 2~35mm, the inner wall diameter of the cylindrical body is 1~34mm, the height of the cylindrical body is 2~9mm, and the thickness of the percolation bottom plate is 0.3~1.0mm; when the cylindrical body is embedded in the holes, the gap distance is 1~3mm.

[0020] This application provides a carrier chamber for cell co-incubation or the carrier chamber for cell co-incubation obtained by the above preparation method and the application of the cell culture device, wherein the carrier chamber or cell culture device is used for co-incubation of cells with hydrogel, fibrous membrane or nanocomposite material.

[0021] In summary, this application provides a carrier chamber for cell co-incubation, the structure of which includes a cylindrical body, a flange, and a percolation base plate; the flange is fixedly assembled to the outer wall of the first end of the cylindrical body, and the percolation base plate is fixedly assembled to the second end port of the cylindrical body; when the carrier chamber is in use, the cylindrical body is embedded in the holes of the external perforated plate, and the flange overlaps the edge of the holes to form an axial limit, so that the cylindrical body and the percolation base plate are suspended in the holes; with the percolation function of the percolation base plate, the exchange of substances between the internal space of the cylindrical body and the external space of the holes can be realized, and based on this structural design, the carrier chamber can support the three-dimensional culture mode of cells.

[0022] This application also provides a method for preparing the above-mentioned carrier chamber for cell co-incubation. The method can be completed by 3D printing or CNC engraving. The preparation process is simple and has a low operating threshold. The parameters can be flexibly adjusted according to experimental needs, and carrier chambers of different specifications (such as cylinder height and flange size) can be produced at any time, effectively adapting to diverse cell co-incubation experimental scenarios.

[0023] Compared with existing technologies, the carrier chamber provided in this application has the following advantages: (i) Better compatibility with the perforated plate: It adopts an integrated design of "cylindrical body embedding and flange limiting", which can directly realize the suspended assembly of the cylindrical body in the perforated plate without the need for additional fixing components. (ii) Support for three-dimensional cell culture: The equipped percolation base plate can effectively ensure the exchange of substances between the inner and outer spaces of the carrier chamber, and can work together with the cylindrical body to build a stable three-dimensional physical environment for cell growth. (iii) Simple and convenient preparation method: The carrier chamber can be quickly produced by 3D printing or CNC engraving process according to the specific specifications of the experiment, without the need for complex molds. Attached Figure Description

[0024] To more clearly illustrate the technical solutions in the embodiments of this application or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this application. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0025] Figure 1 This is a schematic diagram of the structure of a cell co-incubation support chamber provided in an embodiment of this application; wherein the through holes on the permeation substrate are arranged in a honeycomb pattern.

[0026] Figure 2 This is a schematic diagram of the structure of a carrier chamber for cell co-incubation provided in an embodiment of this application; wherein the through holes on the cylindrical body are arranged in a matrix;

[0027] Figure 3 A schematic diagram of the structure of multiple carrier chambers embedded in a 24-well plate provided in the embodiments of this application;

[0028] Figure 4 This is a physical image of the carrier chamber for cell co-incubation provided in Example 3 of this application;

[0029] Figure 5 A flowchart of the cell culture apparatus provided for application examples of this application;

[0030] Reference numerals: 1. Cylindrical main body; 2. Flange; 3. Percolation base plate. Detailed Implementation

[0031] The technical solutions of the embodiments of this application will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some, not all, of the embodiments of this application. Based on the embodiments in this application specification, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection claimed in this application.

[0032] In the description of this application, it should be noted that the terms "center," "upper," "lower," "left," "right," "vertical," "horizontal," "inner," and "outer," etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are used only for the convenience of describing this application and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this application. Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and should not be construed as indicating or implying relative importance.

[0033] Unless otherwise expressly specified and limited, the terms "installation," "connection," and "linking" should be interpreted broadly. For example, they can refer to fixed connections, detachable connections, or integral connections; they can refer to mechanical connections or electrical connections; they can refer to direct connections or indirect connections through an intermediate medium; and they can refer to the internal connection between two components. Those skilled in the art can understand the specific meaning of the above terms in this application based on the specific circumstances.

[0034] The raw materials used in this invention are not particularly restricted in their source; they can be purchased on the market or prepared using conventional methods known to those skilled in the art.

[0035] This application provides a carrier chamber for cell co-incubation, comprising a cylindrical body, a flange, and a percolation bottom plate;

[0036] The outer wall of the first end of the cylindrical body is provided with a flange; the second end of the cylindrical body is provided with a percolation bottom plate. The cylindrical body and the percolation bottom plate enclose an internal chamber, which is used to hold the material.

[0037] The flange is used to overlap the edge of the perforation plate to suspend the internal chamber; the percolation bottom plate allows for material exchange between the internal chamber and the external space.

[0038] In some embodiments, see Figure 1 The cylindrical body 1, flange 2 and percolation base plate 3 are integrally formed; the flange 2 is formed by extending along the outer wall of the first end of the cylindrical body 1 in a direction away from the axis; the percolation base plate 3 is formed by extending along the second end port of the cylindrical body 1 in a direction towards the axis.

[0039] It should be noted that the carrier chamber used for cell co-incubation is prepared using 3D printing or CNC engraving. During the preparation process, the cylindrical body, flange, and percolation base plate of the carrier chamber are integrally formed using the above technology, without the need for subsequent assembly processes.

[0040] In some embodiments, the percolation substrate is provided with through holes, the diameter of which is 0.5 to 2.0 mm; the porosity of the percolation substrate is 15 to 40%.

[0041] In some embodiments, see Figure 2 The cylindrical body is provided with through holes, the diameter of which is 0.5~2.0mm; the opening ratio of the cylindrical body is 15~40%.

[0042] In some preferred embodiments, the diameter of the through hole is 0.5 mm, 1.0 mm, 1.5 mm or 2.0 mm; the opening ratio of the cylindrical body is 15%, 20%, 25%, 30%, 35% or 40%.

[0043] In some preferred embodiments, the through holes are arranged in a matrix or honeycomb pattern.

[0044] It should be noted that by reasonably setting the through-hole size, porosity and through-hole arrangement of the percolation base plate and / or the cylindrical body, the carrying capacity of both can be significantly improved, thereby effectively enhancing the stability of the material inside the carrying chamber; in addition, while ensuring that the overall support performance is not affected, the through-hole diameter and porosity can be further optimized to further increase the material exchange rate between the inner and outer spaces of the cylindrical body.

[0045] In some embodiments, the material of the supporting chamber is one of PTFE, PAEK, PLA, TPU, and PEEK.

[0046] It should be noted that the carrier chambers prepared using the above-mentioned materials, while meeting biocompatibility requirements, also possess excellent sterilization stability and are suitable for various mainstream sterilization methods. For example, PLA can withstand gamma-ray sterilization and ethylene oxide sterilization; PTFE and PAEK can withstand high-pressure steam sterilization, gamma-ray sterilization, and ethylene oxide sterilization. After treatment with the above sterilization methods, the carrier chambers can still maintain their initial structural integrity (no deformation, cracking, or sealing failure) and performance stability (such as no decrease in biocompatibility and filtration efficiency), enabling multiple reuses and effectively reducing experimental costs.

[0047] In some embodiments, the flange is selected from one of a ring-shaped member, a cross-shaped member, and a straight member, and the flange is provided with a snap fastener for connection.

[0048] This application provides a method for preparing a carrier chamber for cell co-incubation, wherein the carrier chamber is prepared by 3D printing or CNC engraving.

[0049] It should be noted that 3D printing or CNC engraving processes are not only simple to manufacture and have low operating thresholds, but also allow for flexible adjustment of parameters according to experimental needs, enabling the production of bearing chambers of different specifications (such as aperture, cylinder height, and flange size) and dimensions, effectively adapting to perforated plates of different specifications.

[0050] This application provides a cell culture device, including a well plate and a support chamber for cell co-incubation;

[0051] One or more holes are provided on the perforated plate; the outer wall diameter of the cylindrical body supporting the chamber is smaller than the inner wall diameter of the hole; the maximum radial dimension of the flange portion supporting the chamber is larger than the inner wall diameter of the hole.

[0052] When the perforated plate is used in conjunction with the support chamber, the cylindrical body of the support chamber is embedded in the hole, and a gap is formed between the outer wall of the cylindrical body and the inner wall of the hole; the flange of the support chamber overlaps the edge of the hole, so that the internal cavity of the support chamber is suspended in the air, and the percolation bottom plate of the support chamber allows for material exchange between the internal cavity and the external space.

[0053] In some preferred embodiments, the dimensional parameters of the perforations on the perforated plate are as follows: the inner wall diameter of the perforation is 3-40 mm, the sidewall thickness is 0.5-1 mm, and the depth is 3-12 mm. This dimensional range covers conventional commercial perforated plate specifications in the art, including but not limited to 6-well, 12-well, 24-well, 48-well, 96-well, and 384-well plates. The dimensional parameters of the supporting chamber are as follows: the outer wall diameter of the cylindrical body is 2-35 mm, the inner wall diameter is 1-34 mm, the height is 2-9 mm, and the thickness of the percolation substrate is 0.3-1.0 mm; when the cylindrical body is embedded in the perforations, the gap distance is 1-3 mm.

[0054] In some embodiments, the cell culture apparatus includes a carrier chamber. When the well plate is used in conjunction with the carrier chamber, the cylindrical body of the carrier chamber is embedded in the hole, and the flange overlaps the edge of the hole so that the internal cavity of the carrier chamber is suspended.

[0055] In some embodiments, see Figure 3 The cell culture device includes multiple carrier chambers. When the well plate is used in conjunction with the carrier chambers, the cylindrical bodies of the multiple carrier chambers are respectively embedded in the holes, and the flanges overlap the edges of the holes to suspend the internal chambers of the carrier chambers.

[0056] In some embodiments, the cell culture apparatus further includes multiple carrier chambers and a well plate with multiple holes. The flanges of the carrier chambers are provided with snap fasteners for connection. Adjacent carrier chambers are connected to each other by snap fasteners on the flanges to form an integral structure. When the well plate is used in conjunction with multiple carrier chambers, the multiple carrier chambers connected to form an integral structure can be picked up and removed simultaneously, effectively improving the convenience of operation.

[0057] This application provides an application of a carrier chamber for cell co-incubation or a carrier chamber for cell co-incubation obtained by the above preparation method and the cell culture device, wherein the carrier chamber or cell culture device is used for co-incubation of cells with hydrogels, fibrous membranes or nanocomposite materials.

[0058] The applicant further provides the following specific embodiments to describe the present invention. It should be noted that these embodiments are merely descriptive and do not limit the present invention in any way.

[0059] Example 1

[0060] This embodiment provides a method for preparing a carrier chamber for cell co-incubation, using PLA as the printing material and a 3D printer to print the carrier chamber. The specific steps are as follows:

[0061] Step S1: In SolidWorks software, complete the model design of the bearing chamber with the following parameters: outer diameter of the cylinder is 32 mm, main body height is 13 mm, and inner cavity diameter of the main body is 30 mm; the flange is an annular part with an outer diameter of 40 mm and an inner diameter of 30 mm; the percolation bottom plate is 1 mm thick, and a through hole with a diameter of 2 mm is opened on the percolation bottom plate, with an opening rate of approximately 30%.

[0062] Step S2, parameter settings: Set the nozzle diameter of the 3D printer to 0.4mm, the layer thickness to 0.10mm (the layer thickness parameter can be reasonably selected within the range of 0.1mm~0.16mm according to actual needs), the infill rate to 100%, the shell wall thickness to no less than 4 line widths, and the printing speed to the range of 40~60mm / s; the specific printing parameters are: nozzle temperature 200℃, heated bed temperature 60℃, layer thickness 0.12mm, infill rate 100%, shell thickness 0.8mm;

[0063] Step S3, Printing Environment and Execution: Printing is performed in a room temperature environment without the need for a sealed hot chamber; the nozzle temperature is controlled between 195 and 210°C, the heated bed is covered with PEI film and maintained at a temperature of 55 to 60°C; the cooling fan is turned off when printing the first layer, and full-speed cooling is started from the second layer onwards. The 3D product is obtained after printing is completed.

[0064] Step S4, Post-processing of the product: After the 3D product has cooled naturally to below 35°C, it is removed; then the support material of the 3D product is removed, the 3D product is chamfered and polished, and finally ultrasonic water washing is performed to remove residual debris to obtain the bearing chamber;

[0065] Step S5, Sterilization and Performance Verification: The carrier chamber was sterilized by a single irradiation with 25 kGy gamma rays, and the size of the carrier chamber did not change significantly. A combined sterilization procedure of "immersion in 75% ethanol for 30 minutes combined with irradiation with 254 nm ultraviolet light for 30 minutes" was performed, and the combined sterilization procedure was repeated 100 times, with the size change rate of the carrier chamber not exceeding 1%.

[0066] Application Example 1

[0067] This application example provides an application of a cell culture device, with the specific steps as follows:

[0068] See Figure 5 Add 2 mL of cell suspension to each well of a 6-well plate; load a 20 mm diameter, 2 mm thick hydrogel into the carrier chamber prepared in Example 1; embed the carrier chamber containing the hydrogel into the 6-well plate containing the cell suspension, and then place it in a cell culture incubator for co-culture.

[0069] Example 2

[0070] This embodiment provides a method for preparing a carrier chamber for cell co-incubation, using PEEK as the printing material and a 3D printer to print the carrier chamber. The specific steps are as follows:

[0071] Step S1, Printing Preparation and Parameter Setting: Place the PEEK printing filament at 120℃ for vacuum drying for no less than 4 hours; then set the 3D printing parameters, including: nozzle diameter of 0.4mm, printing layer thickness of 0.10mm, infill rate of 100%, shell wall thickness of no less than 4 line widths, and printing speed of 20~30mm / s.

[0072] Step S2, High-temperature printing: The printing operation is performed in an industrial-grade 3D printing equipment equipped with a high-temperature nozzle and a closed hot chamber. The nozzle temperature is controlled at 380~400℃, the internal temperature of the printing cavity is 120~150℃, and the hot bed temperature is maintained at 120~160℃. After printing, a 3D product is obtained.

[0073] Step S3, Stress Relief and Post-processing: After the 3D product is slowly cooled to below 80°C in a closed hot chamber, it is taken out; then the support material of the 3D product is manually removed, the 3D product is chamfered and polished, and finally the 3D product is annealed at 200°C for 2 hours to obtain the bearing chamber; in this step, the annealing treatment can improve the crystallinity and dimensional stability of the bearing chamber; after annealing, the bearing chamber can be ultrasonically washed to remove surface dust.

[0074] Step S4, Sterilization and Performance Verification: The carrier chamber is sterilized under high-pressure steam circulation sterilization conditions of 121°C for 15 minutes or 134°C for 3 minutes. The carrier chamber is sterilized repeatedly for no less than 100 times, and its structure and size do not show significant changes.

[0075] In this embodiment, as an alternative sterilization method, gamma-ray irradiation can be used, with the total dose controlled within the range of 25 kGy to 35 kGy. After irradiation, the device must be left to stand for 24 hours before use. Alternatively, ethylene oxide sterilization can be selected. This method must follow the standard cycle parameters of conventional medical device sterilization procedures and ensure sufficient resolution of residual ethylene oxide. For rapid processing requirements in laboratory settings, a combined ethanol-UV sterilization method can be used: the chamber is completely immersed in a 75% ethanol solution for 30 minutes, followed by irradiation with 254 nm UV light for 30 minutes. Experimental verification shows that none of the above three sterilization methods have adverse effects on the structural integrity and functional stability of the device.

[0076] Example 3

[0077] This embodiment provides a method for preparing a carrier chamber for cell co-incubation. The method uses CNC engraving technology to precisely machine PTFE preforms to prepare the carrier chamber, and specifically includes the following steps:

[0078] Step S1, Integrated Structure Machining: A 20 mm diameter USP Class VI pure polytetrafluoroethylene (PTFE) round bar is selected as the base material. On a CNC vertical machining center, milling and precision turning processes are used to complete the precision machining of the outer diameter of the load-bearing chamber, the flange diameter, and the height of the cylinder in one operation. The internal cavity is then drilled on the same machine tool, resulting in an integrated structure of flange, cylindrical body, and base plate. Specific structural parameters are as follows: outer diameter of the cylinder body is 16.3 mm, inner diameter is 14 mm, and height is 16 mm; outer diameter of the flange is 19 mm, and inner diameter is 14 mm. During machining, the coaxiality of all components must be ≤0.02 mm.

[0079] Step S2, Micro-hole fabrication of the base plate: A micro-drill with a diameter of 0.6~1.0 mm or an ultraviolet laser with a wavelength of 355 nm is used to drill holes in the base plate, forming a uniformly distributed array of micro-holes. The total open area ratio of the base plate is controlled between 15~40%, resulting in a bearing chamber. Specific processing parameters are as follows: A 0.8 mm diameter micro-drill is used to process a circumferential array of holes in a 0.6 mm thick permeation base plate, controlling the open area ratio to approximately 25%.

[0080] Step S3, Cleaning and Final Sterilization: The carrier chamber is deburred, then ultrasonically cleaned with pure water for 10 minutes, rinsed with 75% ethanol, and finally autoclaved at 121°C for 15 minutes. After sterilization, it is aseptically dried and packaged for later use.

[0081] In this embodiment, as an alternative sterilization method, gamma-ray irradiation can also be used, with the total dose controlled within the range of 25 kGy to 35 kGy. After irradiation, the device must be left to stand for 24 hours before use. Alternatively, ethylene oxide sterilization can be selected. This method must follow the standard cycle parameters of conventional medical device sterilization procedures and ensure sufficient resolution of residual ethylene oxide. For rapid processing requirements in laboratory settings, a combined ethanol-UV sterilization method can be used: the chamber is completely immersed in a 75% ethanol solution for 30 minutes, followed by irradiation with 254 nm UV light for 30 minutes. Experimental verification shows that none of the above three sterilization methods have adverse effects on the structural integrity and functional stability of the device.

[0082] This embodiment provides a carrier chamber for cell co-incubation obtained using the above-described CNC engraving process. A physical image of this carrier chamber can be found in [reference needed]. Figure 4 . Figure 4 In the middle, the cylindrical body, flange and percolation bottom plate are integrally formed; the flange is formed by extending along the outer wall of the first end of the cylindrical body in a direction away from the axis; the percolation bottom plate is formed by extending along the second end of the cylindrical body in a direction towards the axis, and the percolation bottom plate and the cylindrical body enclose the internal cavity.

[0083] Application Example 2

[0084] This application example provides an application of a cell culture device, with the specific steps as follows:

[0085] Add 2 mL of cell suspension to each well of a 6-well plate; load a 20 mm diameter, 2 mm thick hydrogel into the carrier chamber prepared in Example 3; embed the carrier chamber containing the hydrogel into the 6-well plate containing the cell suspension, and then place it in a cell culture incubator for co-culture.

[0086] The above are merely preferred embodiments of this application and are not intended to limit the present invention. Although this application has been described in detail with reference to examples, those skilled in the art can still modify the technical solutions described in the foregoing examples or make equivalent substitutions for some of the technical features. However, any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of this application should be included within the protection scope of this application.

Claims

1. A carrier chamber for cell co-incubation, characterized in that, Includes a cylindrical main body, a flange section, and a percolation base plate; The first end of the cylindrical body is provided with the flange portion on its outer wall; the second end of the cylindrical body is provided with the percolation bottom plate, and the cylindrical body and the percolation bottom plate enclose an internal cavity, which is used to hold materials. The flange is used to overlap the edge of the perforation plate to suspend the internal chamber; the permeation base plate allows for material exchange between the internal chamber and the external space.

2. The carrier chamber for cell co-incubation according to claim 1, characterized in that, The cylindrical body, the flange, and the percolation base plate are integrally formed; The flange is formed by extending away from the axis along the outer wall of the first end of the cylindrical body; the percolation base plate is formed by extending towards the axis along the second end port of the cylindrical body.

3. The carrier chamber for cell co-incubation according to claim 1, characterized in that: The percolation substrate is provided with through holes, the diameter of which is 0.5~2.0mm; the porosity of the percolation substrate is 15~40%.

4. The carrier chamber for cell co-incubation according to claim 1, characterized in that: The cylindrical body is provided with a through hole, the diameter of which is 0.5~2.0mm; the opening ratio of the cylindrical body is 15~40%.

5. The carrier chamber for cell co-incubation according to any one of claims 2 to 4, characterized in that: The through holes are arranged in a matrix or honeycomb pattern.

6. The carrier chamber for cell co-incubation according to claim 1, characterized in that: The material of the support chamber is one of PTFE, PAEK, PLA, TPU, and PEEK.

7. A method for preparing a carrier chamber for cell co-incubation as described in any one of claims 1 to 6, characterized in that, The supporting chamber is prepared using 3D printing or CNC engraving technology.

8. A cell culture device, characterized in that, Includes well plates and the cell-supporting chambers for co-incubation as described in any one of claims 1 to 6; The perforated plate has one or more holes; the outer wall diameter of the cylindrical body of the supporting chamber is smaller than the inner wall diameter of the hole; the maximum radial dimension of the flange of the supporting chamber is larger than the inner wall diameter of the hole. When the perforated plate is used in conjunction with the bearing chamber, the cylindrical body of the bearing chamber is embedded in the hole, and a gap is formed between the outer wall of the cylindrical body and the inner wall of the hole; the flange of the bearing chamber overlaps the edge of the hole, so that the internal cavity of the bearing chamber is suspended in the air, and the percolation bottom plate of the bearing chamber allows for material exchange between the internal cavity and the external space.

9. The cell culture apparatus according to claim 8, characterized in that: The dimensions of the holes on the perforated plate are as follows: the inner diameter of the hole is 3~40mm, the side wall thickness of the hole is 0.5~1mm, and the depth of the hole is 3~12mm. The dimensions of the carrying chamber are as follows: the outer diameter of the cylindrical body is 2~35mm, the inner diameter of the cylindrical body is 1~34mm, the height of the cylindrical body is 2~9mm, and the thickness of the percolation bottom plate is 0.3~1.0mm. When the cylindrical body is embedded in the hole, the distance of the gap is 1~3mm.

10. The application of a cell co-incubation chamber according to any one of claims 1 to 6, or a cell co-incubation chamber obtained by the preparation method according to claim 7, or a cell culture device according to any one of claims 8 to 9, characterized in that, The carrier chamber or the cell culture device is used for co-incubation of cells with hydrogels, fibrous membranes or nanocomposite materials.

Images