Centrifugal immunoassay device for multiplex diagnosis

Abstract

A centrifugal immunoassay device for multiplex diagnosis comprises: a centrifugal cartridge provided with a plurality of wells for accommodating samples; a rotation driving unit for carrying the centrifugal cartridge and rotating the centrifugal cartridge; an absorbance optical system coupled to the centrifugal cartridge for measuring the absorbance of the object to be detected; a syringe which is located in the centrifugal cartridge and sucks the object to be detected and discharges the object to the detection well; and a housing that encloses the centrifugal cartridge, the rotation drive unit, the absorbance optical system, and the syringe, the absorbance optical system including: a light emission unit that emits detection light of a specific wavelength range toward an object to be detected in the detection well; and a light detection unit for measuring the detection light passing through the object to be detected in the detection well and calculating a detection value, the light detection unit being provided with a plurality of light emission units to emit a plurality of detection light having different wavelength ranges, the light detection unit including a light selection unit, the light selection unit selects detection light having a certain wavelength range from among the plurality of light emission units and emits the detection light to an object to be detected in the detection well.

Description

Technical Field

[0001] This invention relates to a device, specifically a centrifugal immunoassay device for multiple diagnostic purposes. Background Technology

[0002] In existing technologies, the material box is formed as a rotary drive structure for automated measurement and is designed to process samples in various chambers. However, this method relies on rotary rotation, thus requiring a delicate flow path structure and complex engagement of the upper and lower plates. This makes mass production and management difficult, and requires a large number of parts, resulting in high material box costs and sample injection difficulties. Furthermore, the use of an absorbance optical system with a single wavelength limits the ability to perform only one diagnostic procedure.

[0003] Furthermore, in existing immunoassay devices, the centrifuge and transilluminator are set up separately, requiring a step of moving the sample from the centrifuge to the transilluminator, which increases the detection cost and time.

[0004] Therefore, the aim is to use a simple cartridge with multiple wells for centrifugation and transmission detection, and an immunoassay device capable of performing centrifugation and transmission detection in a single unit, for user convenience. Furthermore, the aim is to enable multiple diagnostics using only a single system through a compact, multi-color optical structure.

[0005] Prior art literature

[0006] Patent document: Patent Publication No. 10-2024-0018037 (published on February 13, 2024) Summary of the Invention

[0007] Technical issues

[0008] The technical problem of this invention is to provide a centrifugal immunoassay device having multiple wells for centrifugation and transmission detection in a single cartridge, thereby enabling centrifugation and transmission detection to be performed in a single device. Additionally, it aims to provide a detection system including an optical structure capable of measuring multiple wavelengths for multiplex diagnostics.

[0009] The technical problems of this invention are not limited to those described above, and those skilled in the art can clearly understand from the following description several other technical problems not mentioned.

[0010] Technical solution

[0011] To address the aforementioned technical problems, an embodiment of the present invention provides a centrifugal immunoassay apparatus that detects analytes from samples via centrifugation. The apparatus includes: a centrifugal cartridge with multiple wells for receiving samples; a rotation drive unit for mounting the centrifugal cartridge and rotating it; an absorbance optical system integrated with the centrifugal cartridge for measuring the absorbance of the analytes within the multiple wells; a syringe located within the centrifugal cartridge for drawing in the analytes and discharging them into the wells; and a housing for enclosing the centrifugal cartridge, the rotation drive unit, the absorbance optical system, and the syringe. The absorbance optical system includes: a light emitting unit that emits detection light with a specific wavelength range towards the analytes within the wells; and a light detection unit for measuring the detection light passing through the analytes within the wells and calculating the detection value. Multiple light emitting units are provided to emit multiple detection lights with different wavelength ranges. The absorbance optical system includes a light selection unit capable of selecting detection light with a specific wavelength range from the multiple light emitting units and emitting it towards the analytes within the wells.

[0012] In this embodiment of the invention, the sample may be blood, and the object to be tested may be plasma.

[0013] In this embodiment of the invention, the detection well can be formed as a cuvette structure capable of transmission detection.

[0014] In an embodiment of the present invention, the centrifugal material box may include: a sample well for receiving a sample; a centrifugal separation well, wherein the sample is moved from the sample well by rotation and received in the centrifugal separation well; and a sample movement channel connecting the sample well and the centrifugal separation well to form a flow path.

[0015] In this embodiment of the invention, the syringe may have a suction head component at its lower end for sucking up the test object. The suction head component can penetrate the upper surface of the centrifugal container to suck up the test object stored in the centrifugal separation well and discharge the sucked-up test object into the test well.

[0016] In this embodiment of the invention, a step may be formed at the lower part of the centrifugal separation well so that only the test object separated from the sample by centrifugation can be sucked in. The suction head component can be inserted into the upper part of the step to suck in the test object.

[0017] In embodiments of the present invention, a plurality of light emitting units may be configured in a circular shape, and a light selection unit may include: a drive motor for rotating the plurality of light emitting units configured in a circular shape; and a position sensor for detecting the position of the light emitting units.

[0018] In this embodiment of the invention, the light emitting unit may include: a colored LED for emitting detection light of a specified wavelength; an LED lens for converging the detection light emitted from the colored LED; and a bandpass filter for allowing only light with a specified wavelength range to pass through.

[0019] In this embodiment of the invention, the housing may include at least one of a printer, a touch screen, a progress display, a power switch, and a door.

[0020] In this embodiment of the invention, the touchscreen can be used to receive commands from the user to activate the multiplex diagnostic centrifugal immunoassay device.

[0021] Beneficial effects

[0022] The centrifugal immunoassay apparatus of various embodiments of the present invention utilizes a single centrifugal cartridge for centrifugation and transmission detection, thereby completing the detection process in a single device. Since centrifugation and detection, as well as the confirmation of test results, can be performed using a single centrifugal immunoassay apparatus and a single centrifugal cartridge, it can significantly reduce detection time and costs.

[0023] Furthermore, according to various embodiments of the present invention, the centrifugal immunoassay device can measure multiple wavelengths for multiple diagnostics, thereby reducing testing time and cost.

[0024] The effects of the present invention are not limited to those described above, but should be understood to include all effects that can be derived from the features of the present invention as set forth in the specification and claims. Attached Figure Description

[0025] Figure 1 This is a perspective view of a centrifugal immunoassay apparatus according to an embodiment of the present invention, excluding the outer casing.

[0026] Figure 2 This is a perspective view of a centrifugal feed box according to an embodiment of the present invention.

[0027] Figure 3 This is a cross-sectional view of a centrifugal feed box according to an embodiment of the present invention.

[0028] Figure 4 This is a perspective view illustrating an absorbance optical system according to an embodiment of the present invention.

[0029] Figure 5 This is a conceptual diagram illustrating an absorbance optical system according to an embodiment of the present invention.

[0030] Figure 6 and Figure 7 This is a perspective view illustrating a centrifugal immunoassay apparatus according to an embodiment of the present invention.

[0031] Figure Labels

[0032] 10: Centrifugal Immunoassay Device

[0033] 100: Centrifugal feed box

[0034] 200: Absorbance optical system

[0035] 300: Material box loading section

[0036] 400: Rotary drive unit

[0037] 500: Horizontal drive unit

[0038] 600: Syringe

[0039] 700: Syringe drive unit

[0040] 800: Outer casing Detailed Implementation

[0041] The present invention will now be described with reference to the accompanying drawings. However, the present invention can be implemented in many different forms, and is therefore not limited to the embodiments described herein. Furthermore, in the accompanying drawings, parts unrelated to the description have been omitted for clarity, and similar reference numerals have been used for similar parts throughout the specification.

[0042] Throughout the specification, when a part is "connected (joined, contacted, combined)" with another part, it includes not only "direct connection" but also "indirect connection" with other components in between. Furthermore, when a part "includes" a constituent element, unless explicitly stated otherwise, it does not exclude other constituent elements, but rather allows for the inclusion of other constituent elements.

[0043] The terminology used in this invention is for illustrative purposes only and is not intended to limit the invention. Unless otherwise expressly stated herein, singular expressions include plural expressions. In this invention, terms such as "comprising" or "having" are used to specify the presence of features, numbers, steps, operations, constituent elements, parts, or combinations thereof described in the specification, rather than precluding the possibility of the presence or addition of one or more other features or numbers, steps, operations, constituent elements, parts, or combinations thereof.

[0044] Hereinafter, embodiments of the present invention will be described in detail with reference to the accompanying drawings.

[0045] Figure 1 This is a perspective view of a centrifugal immunoassay apparatus according to an embodiment of the present invention, excluding the outer casing. Figure 2 This is a perspective view of a centrifugal feed box according to an embodiment of the present invention. Figure 3This is a cross-sectional view of a centrifugal feed box according to an embodiment of the present invention. Figure 4 This is a perspective view illustrating an absorbance optical system according to an embodiment of the present invention. Figure 5 This is a conceptual diagram illustrating an absorbance optical system according to an embodiment of the present invention. Figure 6 and Figure 7 This is a perspective view illustrating a centrifugal immunoassay apparatus according to an embodiment of the present invention.

[0046] Reference Figures 1 to 7 In one embodiment of the present invention, the centrifugal immunoassay apparatus 10 can be a device for separating a target analyte from a sample and detecting the separated target analyte. In this case, the centrifugal immunoassay apparatus 10 may include a centrifugal cartridge 100, an absorbance optical system 200, a cartridge loading unit 300, a rotation drive unit 400, a horizontal drive unit 500, a syringe 600, a syringe drive unit 700, a housing 800, and a control unit.

[0047] The centrifugal cartridge 100 can be an all-in-one cartridge mounted on the centrifugal immunoassay apparatus 10 to separate the analyte from the collected sample by rotation and to perform detection on the separated analyte. In this case, the centrifugal cartridge 100 may include a sample well 110, a centrifugal separation well 120, a sample movement channel 130, a detection well 140, a cover 150, and a cap 160.

[0048] Before centrifugation, the user injects a sample, and the sample well 110 can be a space for receiving the injected sample. The sample can be whole blood (B). As another example, the sample can be a urine sample.

[0049] The sample housed in the sample well 110 is moved by a rotational operation, and the centrifugal separation well 120 can be a space for housing the sample in a separated state. More specifically, the centrifugal separation well 120 can be configured separately from the sample well 110, and the centrifugal separation well 120 can be a space where the sample housed in the sample well 110 is moved and housed by the centrifugal force generated by the rotational operation. Additionally, the centrifugal separation well 120 can be a space for housing a sample in a centrifugally separated state, wherein the sample has been centrifugally separated according to the specific gravity of the substances constituting the sample.

[0050] At this time, the sample stored in the sample well 110 can move to the centrifugal separation well 120 through the sample moving channel 130. The sample moving channel 130 is a flow path formed by connecting the sample well 110 and the centrifugal separation well 120, and can be a channel that allows the sample to move only from the sample well 110 to the centrifugal separation well 120. At this time, the sample moving channel 130 can be formed at a position above a preset height to prevent the centrifuged sample from moving back to the sample well 110. The preset height can be the height of the sample stored in the sample well 110 before the centrifugal separation operation.

[0051] In a centrifuged sample, the heavier substances may settle at the bottom of the centrifuge well 120, while the lighter substances may lie above the heavier substances, thus separating them into layers. As one embodiment, the lighter substance in the centrifuged sample may be the target analyte, specifically plasma (P). As another embodiment, the heavier substance in the centrifuged sample may be a non-target analyte sample, such as erythrocytes (R).

[0052] The centrifuge well 120 may have a step 121 formed at its lower part so as to draw in only the analyte. More specifically, the centrifuge well 120 may have a step 121 formed at its lower part so as to draw in only the analyte from the sample in a centrifuged state.

[0053] Step 121 can be a space for drawing the sample to be tested, contained in the centrifugal separation well 120, into the suction head component 610 (described later). Step 121 can be a space where the suction head component is inserted at the top to extract the sample to be tested. Step 121 can be formed at a predetermined position at the top, with only the sample containing the lower specific gravity of the centrifuged sample being placed there. In this case, the predetermined position can be a position higher than the height of the precipitated sample with the higher specific gravity.

[0054] The centrifugal separation well 120, by forming a step 121, ensures that the suction head component 610 only draws in the object to be measured, thereby ensuring reproducibility and improving the accuracy of the test. Reproducibility refers to the degree to which the measurement results are consistent when the same object is measured using the same method under any different conditions of the measurer, device, measurement location, or measurement time.

[0055] The detection well 140 is a space for receiving and storing the test object in the centrifugal separation well 120 for testing. More specifically, the suction head component 610 that sucks in the test object stored in the centrifugal separation well 120 ejects the test object, and the detection well 140 is used to collect the ejected test object.

[0056] The detection well 140 can be formed as a cuvette structure to enable transmission detection. The cuvette can be a small tubular container designed for storing a sample in spectrophotometric measurements. The detection well 140 can be formed of a transparent material to allow light generated by the absorbance optical system 200 (described later) to pass through the object being measured contained within the detection well 140 to measure absorbance.

[0057] As one embodiment, the detection well 140 can be a plastic cuvette. Plastic cuvettes can be used for high-speed spectrophotometric analysis where speed is more important than accuracy, and can be discarded after use to prevent contamination during reuse. Furthermore, plastic cuvettes can be made of polymethyl methacrylate (PMMA) or polystyrene (PS), thus being manufactured at a lower cost compared to existing containers. However, the invention is not limited to the above embodiments. As another embodiment, the detection well 140 can be a glass cuvette. As yet another embodiment, the detection well 140 can be a quartz cuvette. In this case, quartz cuvettes have better durability than plastic or glass cuvettes, and can transmit a wider range of wavelengths of light compared to plastic or glass cuvettes.

[0058] The cover 150 can be configured to seal the upper surface of the centrifugal container 100. The cover 150 seals the upper surface of the centrifugal container 100 to prevent the sample contained in the centrifugal container 100 from detaching during centrifugation. In this case, the cover 150 can seal the upper surface of the centrifugal container 100 except for the sample well 110 where the sample is injected before centrifugation.

[0059] The cover 150 may be formed of a material through which the suction head component 610 penetrates. As one embodiment, the cover 150 may be an aluminum cover formed by hot melting. However, the invention is not limited thereto, and the cover 150 may be formed of a material that can be penetrated by the suction head component 610.

[0060] The cap 160 is capable of opening and closing the sample well 110. Specifically, the cap 160 can be disposed on the upper surface of the centrifugal collection box 100 to open and close the sample well 110, thereby controlling the movement of substances entering and leaving the sample well 110. As one embodiment, the cap 160 can be separated from the sample well 110 before centrifugation to inject the sample into the sample well 110. As another embodiment, the cap 160 can be engaged with the sample well 110 during centrifugation to prevent the sample from detaching from the sample well 110.

[0061] The absorbance optical system 200 can be combined with the detection well 140 into which the object to be detected is injected to perform transmission detection of the injected object. The absorbance optical system 200 allows light to pass through the detection well 140 of the cuvette structure to measure the absorbance of the object to be detected. As one embodiment, the absorbance optical system 200 may include multiple LED lenses 210 with different wavelengths, a colored LED 220, and a bandpass filter 230. The detection light emitted from the colored LED 220 passes sequentially through the LED lens 210 and the bandpass filter 230, thereby having a predetermined wavelength. The LED lens 210, the colored LED 220, and the bandpass filter 230 are grouped together to form a light emitting section for emitting detection light with a single wavelength. As one embodiment, the multiple light emitting sections for emitting detection light with different wavelengths may be configured in a circular shape. Detection can be performed using a light selection section including a drive motor 240 and a position sensor 250, and using the detection light emitted from one of the multiple light emitting sections.

[0062] In this way, by using detection light of a predetermined wavelength emitted from any one of the multiple light emitting units, one component can be diagnosed, and when using a light selection unit to use detection light of other wavelengths, other components can be diagnosed.

[0063] The dichroic filter 260 allows only the wavelength of light most absorbed by the object being detected to pass through. More specifically, the dichroic filter 260 may be a special filter designed to reflect light with excitation wavelengths and allow light with emission wavelengths to pass through. Furthermore, the absorbance optical system 200 may include a monitoring photodiode 270 to monitor the wavelength of the light reflected by the dichroic filter 260, thereby controlling the detection light emitted from the light emitting unit to a predetermined intensity.

[0064] Light passing through the dichroic filter 260 can be reflected by the mirror 280 and then pass through the detection well containing the object to be detected. The light passing through the detection well can be used by a photodetector, including a photodiode 290, to calculate the detection value.

[0065] The cartridge loading section 300 can be a device for housing the centrifugal cartridge 100 and the absorbance optical system 200. More specifically, the cartridge loading section 300 may have a cartridge opening larger than that of the centrifugal cartridge 100 to allow the centrifugal cartridge 100 to rotate. Additionally, the cartridge loading section 300 can be a space for combining the centrifugal cartridge 100 (after centrifugation) with the absorbance optical system 200 to measure the absorbance of the object being tested.

[0066] The rotary drive unit 400 is a rotating device disposed inside the material box loading unit 300, capable of carrying the centrifugal material box 100 and performing centrifugal separation. In one embodiment, the rotary drive unit 400 can rotate at a high speed of 4000 rpm. The rotary drive unit 400 can rotate at high speed to move the sample housed in the sample well 110 through the sample moving channel 130 to the centrifugal separation well 120, and can separate the sample moved to the centrifugal separation well 120 into test sample and non-test sample.

[0067] In another embodiment, the rotary drive unit 400 can perform step-by-step rotation. More specifically, the rotary drive unit 400 can perform step-by-step rotation such that the center of the suction head component 610 is aligned with the center of the centrifugal separation well 120 when the suction head component 610 is inserted into the centrifugal separation well 120 to suck up the object to be tested. Furthermore, when the suction head component 610 sucks up the object to be tested and discharges it into the detection well 140, the rotary drive unit 400 can perform step-by-step rotation to align the center of the suction head component 610 with the center of the detection well 140.

[0068] The horizontal drive unit 500 may be located at the lower part of the cartridge loading unit 300 to move the cartridge loading unit 300 in a horizontal direction. More specifically, when the cartridge loading unit 300 is viewed from the front (e.g., a direction parallel to the ZY plane), the horizontal drive unit 500 may be disposed on either side of the two side surfaces of the cartridge loading unit 300 to move the cartridge loading unit 300 in a horizontal direction (e.g., the X-axis direction or the -X-axis direction). In this case, the horizontal drive unit 500 may have a guide rail for moving the cartridge loading unit 300 in a horizontal direction.

[0069] The guide rail can be formed to extend parallel to the moving direction (X-axis direction) of the horizontal drive unit 500. In this case, a guide protrusion protruding in the lateral direction (Y-axis direction) can be formed at the lower side end of the material box loading unit 300. As one embodiment, the guide protrusion can extend along the circumferential direction of the lower side end of the material box loading unit 300 to connect with the guide rail.

[0070] In another embodiment, the guide protrusion extends along the circumferential direction of the lower side end of the cartridge loading portion 300, or it may only be formed in a portion of the circumference of the lower side end of the cartridge loading portion 300. In this case, the guide rail may extend parallel to the guide protrusion and be configured such that one surface of the guide rail (hereinafter, the guide surface) faces one surface of the guide protrusion. Therefore, when the cartridge loading portion 300 moves in the horizontal direction (X-axis direction or -X-axis direction), the guide protrusion can slide along the guide surface of the guide rail, thereby preventing the cartridge loading portion 300 from tilting.

[0071] The syringe 600 is disposed on the upper part of the material loading section 300 and is capable of drawing in the test object and injecting it into the detection well 140. More specifically, the syringe 600 can be inserted into the upper part of the step 121 formed in the lower part of the centrifugal separation well 120 and draw in the test object, and then discharge the drawn-in test object into the detection well 140. In this case, the syringe 600 may include a suction head component 610.

[0072] The suction tip 610 is disposed at the lower end of the syringe 600 and is used for drawing in and discharging the test object. The suction tip 610 can penetrate the cover 150 on the upper surface of the centrifugal cartridge 100 to draw in the test object contained in the centrifugal separation well 120. In this case, the suction tip 610 can be formed with a smaller diameter towards the end to penetrate the cover 150. Afterwards, the suction tip 610 can discharge the drawn-in test object into the detection well 140, which serves as a space for absorbance measurement.

[0073] The syringe drive unit 700 enables the syringe 600 to move. More specifically, the syringe drive unit 700 can be driven in a vertical direction (e.g., the Z-axis direction or the -Z-axis direction) to cause the syringe 600 to draw in and expel the test object. As one embodiment, the syringe drive unit 700 can be driven downward (in the -Z-axis direction) to cause the syringe 600 to draw in the test object housed in the centrifugal separation well 120. As another embodiment, the syringe drive unit 700 can be driven upward (in the Z-axis direction) to cause the syringe 600 to expel the drawn-in test object into the detection well 140.

[0074] The housing 800 can cover the centrifugal cartridge 100, the absorbance optical system 200, the cartridge loading unit 300, the rotary drive unit 400, the horizontal drive unit 500, the syringe 600, and the syringe drive unit 700. At this time, the housing 800 may include a printer 810, a touch screen 820, a progress display 830, a power switch 840, and a door 850.

[0075] Printer 810 may be a thermal printer that applies heat to thermal paper to display text or images. More specifically, printer 810 is a thermal printer that functions as a non-impact printing device, and a thermal printer may be a printer that heats the thermal paper by a printhead to print text or images in a dot-matrix manner. However, the invention is not limited thereto. In this case, when the housing 800 is viewed from the front (e.g., a direction parallel to the ZY plane), printer 810 may be positioned at the upper end of the housing 800.

[0076] The touchscreen 820 can be used to receive commands from the user to operate the centrifugal immunoassay device 10. The touchscreen 820 can be a contact display device that allows commands to be input via a part of the user's body, such as a finger or palm, and a touch-sensitive unit.

[0077] The touchscreen 820 may have command input touch keys to enable the centrifugal immunoassay apparatus 10 to perform multiple actions. As one embodiment, the touchscreen 820 may have a start key and a pause key. However, the invention is not limited thereto. As another embodiment, the touchscreen 820 may have a rotation operation key for centrifugation via the rotation drive unit 400, and a detection operation key for moving the centrifuged test object to the detection well 140 and performing the detection operation.

[0078] The progress display 830 may be configured on the surface of the housing 800 as viewed from the front (e.g., in a direction parallel to the ZY plane) and displayed outwards so that the user can confirm the extent of operation of the centrifugal immunoassay device 10.

[0079] The power switch 840 can be a switch for turning the power supply of the centrifugal immunoassay apparatus 10 on or off. The power switch 840 can be disposed on a surface of the housing 800 as viewed from the front (e.g., in a direction parallel to the ZY plane).

[0080] The door 850 can be opened and closed in a manner that allows the user to mount the centrifugal cartridge 100 onto the centrifugal immunoassay apparatus 10. At this time, the door 850 can be configured on the surface of the housing 800 as viewed from the front direction (e.g., in a direction parallel to the ZY plane).

[0081] The control unit can control the operation of the centrifugal immunoassay apparatus 10 based on commands input by the user via the touchscreen 820. More specifically, the control unit can control the absorbance optical system 200, the rotation drive unit 400, the horizontal drive unit 500, the syringe 600, and the syringe drive unit 700 based on commands input by the user via the touchscreen 820.

[0082] As one embodiment, when the start button on the touch screen 820 is pressed, the absorbance optical system 200, the rotation drive unit 400, the horizontal drive unit 500, the syringe 600, and the syringe drive unit 700 can be controlled to cause the centrifugal immunoassay device 10 to perform the following operations: centrifuging the sample by rotating the rotation drive unit 400; moving the syringe 600 up or down by moving the syringe drive unit 700; sucking in and dispensing the test object by the suction tip component 610 of the syringe 600; and combining the centrifugal cartridge 100 with the absorbance optical system 200 to detect the test object.

[0083] In addition, if a problem occurs during the operation of the centrifugal immunoassay apparatus 10, the pause button on the touch screen 820 can be pressed to pause multiple operations being performed by the centrifugal immunoassay apparatus 10.

[0084] The centrifugal immunoassay apparatus 10 of the present invention, as described above, enables centrifugation and transmission detection using a single centrifugal cartridge 100, thereby completing the detection process in a single device. Since centrifugation and detection, and confirmation of test results, can be performed using only one centrifugal immunoassay apparatus 10 and one centrifugal cartridge 100, detection time and costs can be significantly reduced. Furthermore, by selectively using multiple wavelengths of detection light, multiple diagnostics can be performed within a single detection system.

[0085] The foregoing description of the present invention is for illustrative purposes only, and those skilled in the art can easily modify it into other specific forms without changing the technical concept or essential features of the invention. Therefore, it should be understood that the various embodiments described above are exemplary and not limiting in all respects. For example, the individual constituent elements described as integral can be implemented in a discrete manner, and similarly, the multiple constituent elements described as discrete can be implemented in a combined manner.

[0086] The scope of this invention is defined by the appended claims, and all modifications or variations derived from the meaning, scope and equivalent concepts of the claims are within the scope of this invention.

Claims

1. A centrifugal immunoassay device for multiplex diagnostics, which detects the analyte from a sample by centrifugation, comprising: Centrifugal sample box with multiple wells for storing samples; A rotary drive unit is used to mount a centrifugal material box and perform a rotational motion. An absorbance optical system, combined with a centrifugal cartridge, is used to measure the absorbance of the object being tested in multiple test wells; The syringe, located in the centrifugal cartridge, is used to draw in the object to be tested and eject it into the detection well; the outer shell is used to enclose the centrifugal cartridge, the rotary drive unit, the absorbance optical system, and the syringe. Its features are, The absorbance optical system includes: a light emitting unit that emits detection light with a specific wavelength range toward the object to be detected inside the detection well; and a light detection unit that measures the detection light passing through the object to be detected inside the detection well and calculates the detection value. It is equipped with multiple light emitting units to emit multiple detection lights with different wavelength ranges. The absorbance optical system also includes a light selection unit, which can select detection light with a certain wavelength range from multiple light emitting units and emit it to the detection object in the detection well.

2. The centrifugal immunoassay device for multiplex diagnosis according to claim 1, characterized in that, The sample is blood, and the object of the test is plasma.

3. The centrifugal immunoassay device for multiplex diagnosis according to claim 1, characterized in that, The detection well is formed into a cuvette structure capable of transmission detection.

4. The centrifugal immunoassay device for multiplex diagnosis according to claim 1, characterized in that, Centrifugal feed box features: Sample well, used to store samples; A centrifugal separation well, in which the sample is moved from and contained within the sample well by rotation; and The sample movement channel connects the sample well to the centrifugal separation well to form a flow path.

5. The centrifugal immunoassay device for multiplex diagnosis according to claim 4, characterized in that, The syringe has a suction tip at its lower end for drawing in the object to be tested. The suction head component penetrates the upper surface of the centrifugal material box to suck in the test object stored in the centrifugal separation well, and then discharges the sucked-in test object into the test well.

6. The centrifugal immunoassay device for multiplex diagnosis according to claim 5, characterized in that, A step is formed at the bottom of the centrifuge well to ensure that only the analyte separated from the sample by centrifugation is drawn in. The suction head is inserted into the upper part of the step to suck up the object to be tested.

7. The centrifugal immunoassay device for multiplex diagnosis according to claim 1, characterized in that, Multiple light emitting units are arranged in a circular pattern. The light selection unit includes: a drive motor for rotating a plurality of light emitting units configured in a circular shape; and a position sensor for detecting the position of the light emitting units.

8. The centrifugal immunoassay device for multiplex diagnosis according to claim 1, characterized in that, The light emitting unit includes: a colored LED for emitting detection light of a specified wavelength; an LED lens for focusing the detection light emitted from the colored LED; and a bandpass filter for allowing only light within a specified wavelength range to pass through.

9. The centrifugal immunoassay device for multiplex diagnosis according to claim 1, characterized in that, The housing includes at least one of a printer, a touch screen, a progress display, a power switch, and a door.

10. The centrifugal immunoassay device for multiplex diagnosis according to claim 9, characterized in that, The touchscreen is used to receive commands from the user to operate the multiplex diagnostic centrifugal immunoassay device.

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